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Impacts Of Artificial Habitat Structures On Overwinter Fish Survival And Fish Presence In South-Central Nebraska, Logan Dietrich

Biology Theses, Dissertations, and Student Creative Activity

Artificial lentic systems, such as borrow pits, often lack structural complexity due to construction practices and steep bathymetry, limiting features like vegetation and woody debris essential for fish communities. To address this, artificial structures made of materials such as polyvinyl chloride are increasingly used by state and federal agencies to supplement habitat. However, little information is known about the impact these structures have on fish communities. Thus, the objectives of this study were to examine the influence of Georgia cubes in borrow pit impoundments on overwinter survival of sub-stock and ≥ stock Bluegill (Lepomis macrochirus) and Largemouth Bass …

Human Development, Inequality, And Their Associations With Brain Structure Across 29 Countries, Vicente Medel, Luz M. Alliende, Richard Bethlehem, Jakob Seidlitz, Grace Ringlein, Celso Arango, Aurina Arnatkevičiūtė, Laila Asmal, Mark Bellgrove, Anderson M. Winkler

Human Genetics Publications

Background: The macro-social and environmental conditions in which people live, such as the level of a country’s development or inequality, are associated with brain-related disorders. However, the relationship between these systemic environmental factors and the brain remains unclear. We here aimed to determine the association between the level of development and inequality of a country and the brain structure of healthy adults.

Methods: We conducted a cross-sectional study pooling brain imaging (T1-based) data from 145 magnetic resonance imaging (MRI) studies in 7,962 healthy adults (4,110 women) in 29 different countries. We used a meta-regression approach to relate the brain structure …

Role And Electrophysiological Properties Of Sexually Dimorphic Oxytocin Receptor Expressing Neurons In The Anteroventral Periventricular Nucleus Of The Female Mice, Armita Abdollahi Govar

LSU Doctoral Dissertations

Oxytocin is a neurohypophyseal hormone that plays a crucial role in regulating mother-infant bonding and maternal behavior. Oxytocin modulates sex-specific parental behaviors by binding to the oxytocin receptor (OXTR)-expressing neurons in the brain. This study investigates the sexually dimorphic OXTR neurons in the anteroventral periventricular nucleus (AVPV) to understand how oxytocin modulates behavior in females. Specifically, we explored the behavioral roles, neural projections, and intrinsic electrophysiological characteristics of these neurons in the female mice. Using immunohistochemistry revealed that the number of OXTR-expressing neurons was enhanced in postpartum females compared with virgin females, suggesting that these neurons are involved in female-specific …

Evaluation Of Diy Pest Management Strategies For The German Cockroach (Blattella Germanica) In The Presence Of Vitamin K Precursors And Synthetic Amorphous Silica, Mohamed S. Shaik

LSU Master's Theses

The German cockroach, Blattella germanica (Linnaeus) is a globally distributed indoor dwelling pest of medical concern. Insecticides are the preferred method of cockroach management due to their low cost, quick action, and availability. However, after continuous use, the development of insecticide resistance has emerged as a challenge for the pest management industry to effectively eliminate German cockroaches. Integrated pest management (IPM) is a sustainable framework which includes the use of glue board traps, vacuuming, in combination with different insecticide formulations such as sprays, gel or dust baits, and insecticide rotation has been successful for the management of German cockroaches. This …

Maternal Hyperleptinemia Alters Placental Transcriptome And Progesterone Regulation Without Affecting Early Equine Fetal Growth, Lucinda L. Thorburn

LSU Master's Theses

While consequences of equine obesity are well defined, the extent to which persistent hyperleptinemia and accompanying leptin (Lep) resistance affect the fetal-maternal environment warrants further exploration. This study compared endocrine, fetal, placental, and uterine parameters during early pregnancy (through day 70) in mares with elevated leptin versus normal leptin (NL) concentrations. Fourteen mares were classified as either NL (n = 6; Lep 0.9 ± 0.3 ng/mL), moderate leptin (ML; n = 4; Lep 3.0 ± 0.3 ng/mL), or high leptin (HL; n = 4; Lep 5.8 ± 0.7 ng/mL) based on annual plasma leptin. Mares were artificially inseminated and induced …

Aeroponic System Optimization For Butterhead Lettuce Growth And Future Sustainability Using Flow Blurring Atomization: An Overview, Taylor J. Johnson

Beyond: Undergraduate Research Journal

The global population has grown by 6 billion people over the last century and is trending toward 9.7 billion people by the year 2050. Agriculture accounts for 70% of global fresh water usage. Technology must be developed to accommodate the increase of food production demanded by the growing global population and the subsequent increase in water usage. Aeroponic technology is a water-efficient vertical farming technology that can reduce water usage by 90% by suspending plant roots in air within a controlled chamber and supplying atomized droplets of a water-nutrient solution directly to the roots.

This study simultaneously tests six droplet …

Examining Weight Bias Attitude Changes After Exposure To A Brief Photovoice Assignment, Gretchen L. George, Hillary L. Roberts, Kristin Rose, Madison Clemens, Kate G. Burt

Journal of Dietetic Education

Weight bias is present among pre-health professional students (e.g., nutrition, dietetics, nursing, physical therapy, medicine). Educators need support and guidelines about how to enhance learning through engaging assignments while also meeting accreditation requirements. This pilot study examined the impact of an innovative weight bias assignment using photovoice and reflection within existing nutrition education courses, measured through pre-post assessments from the Fat Attitudes Assessment Toolkit. While not all subscales showed significant change, improvements in the Fat Acceptance and Attribution Complexity Composites suggest these methods may be effective in shifting attitudes about fatness. Further research is needed to assess the long-term impact …

Development Of Dietetics Students’ Skills And Self-Efficacy In The Nutrition Care Process, Kristen Heitman, Marcia Nahikian-Nelms, Holly Estes-Doetsch, Kristen M. Roberts, Jennifer A. Garner, Julie Kennel, Christopher A. Taylor

Journal of Dietetic Education

The Nutrition Care Process (NCP) is a systematic framework for providing nutrition care and a cornerstone of dietetics practice. Dietetics education relies on development of skills to achieve competence and demonstrate readiness for practice. The relationship between development of fundamental skills and self-efficacy among dietetics students preparing for clinical practice remains unclear. The purpose of this study was to examine the development of dietetics students’ skills in NCP documentation and their self-efficacy in performing the skills over one academic year. Student nutrition notes (n = 120 notes, n = 30 students) from standardized patient encounters were evaluated for quality using …

Burnout And Turnover Among Program Directors In Acend-Accredited Registered Dietitian Nutritionist Programs, Heather Frazier, Patricia Grace-Farfaglia

Journal of Dietetic Education

Background: Knowledge about program director burnout in ACEND-accredited registered dietitian nutritionist programs is severely limited. Objective: To measure the changes in program director burnout and turnover over a two-year period. Methods: An online questionnaire was distributed to program directors via e-mail in 2021 and 2023. The validated Maslach Burnout Inventory-Educators Survey (MBI-ES) was used to measure burnout, which had three scales: emotional exhaustion, depersonalization, and personal accomplishment. Demographic and program information was also obtained. Results: Over the two-year period, program directors scores for emotional exhaustion (p < 0.001) and depersonalization (p < 0.001) significantly worsened. Interestingly, personal accomplishment scores significantly improved (p < 0.001) during the same time frame. Sixty-six percent of program directors in 2023 reported consideration for leaving their position in the next 5 years, which increased from 58%. Since 2021, 29.9% Didactic Program in Dietetics directors, 28.8% Dietetic Internship directors, and 37.5% Coordinated Program in Dietetics directors have resigned. Conclusion: Program directors show increased signs of burnout. Efforts are needed to investigate ways to support program directors to reduce burnout and increase retention of program directors.

Integrated Advanced Education Supports Future Registered Dietitian Nutritionists’ Breastfeeding Self-Efficacy In Practice, Crystal C. Douglas, Simone Camel

Journal of Dietetic Education

Objective: Evaluate the effectiveness of graduate-level breastfeeding and lactation management education that includes didactive and active learning components on future RDNs’ knowledge, attitudes, and self-efficacy. Design: A pre-and post-test design, utilizing a 41-item pre-intervention survey, learning modules, and a 35-item post-intervention survey. Setting: Three U.S. public universities located in Texas, Louisiana, and Arkansas. Participants: Graduate-level dietetic internship students. Intervention: Four advanced education modules (2 didactive; 2 active learning) developed by Registered Dietitian Nutritionists. Main outcome measures: Gains in breastfeeding knowledge, attitudes, and self-efficacy to assist with breastfeeding education and problem-solving using validated and newly developed survey items. Analysis: Descriptive statistics, …

Sense Of Belonging In The Profession And Program Of Study Among Nutrition And Dietetics Students, Jessica Torro, Melissa Masters, Rachel C. Sinley, Jesse Lunsford, Heidi Masching, Miranda Chen Musgrove

Journal of Dietetic Education

Sense of belonging may positively impact student performance, especially in marginalized groups. This study aimed to examine sense of belonging among a diverse group of nutrition and dietetics students. An online survey investigated students’ feelings of belonging, isolation, and connection to the field of nutrition and dietetics and department of study. The survey was distributed through email to the nutrition and dietetics student body in the Department of Nutrition at Metropolitan State University of Denver. Quantitative data were analyzed as count and percentage. Overall sense of belonging scores were calculated as an average on a 5-point scale (1-strongly disagree to …

Cover, Table Of Contents, About The Journal, Diana Cuy Castellanos, M Elizabeth (Beth) Miller

Journal of Dietetic Education

No abstract provided.

Editors' Message, Diana Cuy Castellanos, M Elizabeth (Beth) Miller

Journal of Dietetic Education

No abstract provided.

Preparation Of Human Asprosin Nanobodies Based On A Natural Nanobody Phage Display Library, Yun Liang, Yi Huang, Qinghua He

Journal of Food Science and Biotechnology

[Objective ] Asprosin (ASP ),an adipokine produced from fibrillin- 1 (FBN 1) cleavage,is closely linked to type 2 diabetes mellitus and obesity when its level is abnormal,demonstrating significant effect on human health.Therefore,monitoring the ASP level is crucial.The author aims to screen the nanobodies specifically binding to ASP via phage display technology,offering a new antibody component for the research on ASP.[Method ] The antigenic epitopes of ASP were predicted by the online tools ABCpred and NovoPro,and then coupled with bovine serum albumin (BSA ) to form the coating antigen (ASP-Epitope-BSA ).After five rounds of bio-panning,the nanobodies specifically binding to ASP were …

Modulatory Effects Of Flavonoids On Il- 4/Il-13 In Atopic Dermatitis And Their Structure-Activity Relationships, Yueyuan Wang, Jing Liu, Chen Li, Fengfeng Chen, Cheng Yang, Bingtian Zhao

Journal of Food Science and Biotechnology

[Objective ] This study aims to explore the ameliorating effects of flavonoids on atopic dermatitis (AD) and their structure-activity relationships.[Method ] Flavonoids were selected through network pharmacology with interleukin- 4 (IL-4) and interleukin- 13 (IL-13) as key targets.The immunoglobulin E (IgE)-induced RBL- 2H3 cell model was employed to evaluate the effects of flavonoids on inflammatory factors,degranulation activities,and calcium ion influx,and the structure-activity relationships were analyzed.Finally,molecular docking was utilized to assist in exploring the action mechanism.[Result ] All of the six flavonoids (luteolin,apigenin,chrysin,quercetin,kaempferol,and fisetin ) screened by network pharmacology had significant inhibitory effects on the expression of IL- 4,IL- 13,tumor necrosis …

Structural And Solution Property Characterization Of Oat Β-Glucans, Hongli Zhang, Yaning Shi, Ming Miao

Journal of Food Science and Biotechnology

[Objective ] This study aims to characterize the structure and investigate the solution properties of oat β-glucan (OBG ).[Method ] Two soluble OBGs with different weigh-average molecular weights (Mw),number-average molar weights (Mn),and peak molecular weights (Mp) were prepared by hot water extraction and ethanol classification.The structures and solution properties were characterized by Fourier-transform infrared spectroscopy (FT-IR ),nuclear magnetic resonance (NMR ),X-ray diffraction (XRD ),scanning electron microscopy (SEM ),high performance size exclusion chromatography coupled with multi-angle laser light scattering (HPSEC-MALLS ),and rheology.[Result ] OBG- 1 and OBG- 2 showed the Mw of 80.73×104 g/mol and 8.05×104 g/mol,the yields of (4.86±0.55)% and …

Research And Preliminary Application Of A Rapid Immunoassay Method For Eugenol Based On Quantum Dots, Junjie Sun, Xiaoxia Liu, Ziyi Chen, Yinlai Li, Lu Zhai, Qinzhou Chen, Renyao Jin

Journal of Food Science and Biotechnology

[Objective ] This study aims to establish a rapid immunoassay method for the quantitative detection of eugenol.[Method ] The eugenol monoclonal antibody was conjugated to the surface of quantum dots by the 1-ethyl- 3- (3-dimethylaminopropyl ) carbodiimide (EDC ) and N-hydroxy-succinamide (NHS ) (EDC-NHS ) coupling method,with quantum dots as fluorescent markers.Bovine serum albumin (BSA ) was used for blocking,and fluorescence microscopy,agarose gel electrophoresis,and a microplate reader were used for identification.Furthermore,the established method was compared with high performance liquid chromatography (HPLC ).[Result ] The optimal synthesis conditions were as follows:quantum dot activation time of 30 min,conjugation pH with 8.0,antibody addition …

Effects Of Tea Polysaccharides On The Properties Of Chestnut Starch, Lei Lei, Wenjun Fu, Lingling Yang, Haibo Liu, Daxi Sang, Qing Wang

Journal of Food Science and Biotechnology

[Objective ] This study aims to improve the properties of chestnut starch.[Method ] Tea polysaccharides were added to chestnut starch in different proportions,and the effects of tea polysaccharides on the water-holding capacity,digestive properties,textural properties,and other properties of chestnut starch were investigated.[Result ] The water-holding and oil-holding properties of chestnut starch were the largest when tea polysaccharides were added at 1% and then gradually decreased with the increase in the proportion of tea polysaccharides added.The transparency of chestnut starch gradually increased when tea polysaccharides were added from 1% to 8% and decreased significantly when tea polysaccharides were added at 16%.Adding tea …

Research Progress In Thermo-Reversible Polysaccharide Gels, Dongxin Zhou, Ziao Zhang, Su Chen, Weimin Yang, Jiajun Sun, Shengzu Han, Anning Xie, Xiaoxiao Li, Yuxiang Bai

Journal of Food Science and Biotechnology

Thermo-reversible gels are widely applied in the processing of baking,meat,and snack products including cakes,candies,jelly,aspic,and steamed custard buns.Currently,the most effective thermo-reversible gel is gelatin,which can combine with water to form a transparent gel that melts when it is close to the temperature of the human oral cavity.However,gelatin is expensive.Therefore,researchers have turned their attention to polysaccharide gels,hoping to obtain more thermo-reversible food raw materials with different properties.The author reviews thermo-reversible polysaccharide gels from natural sources such as plants,algae,and microorganisms and those modified by physical,chemical,and enzymatic methods and summarizes the sources,structures,gelatinization mechanisms,and product applications of polysaccharide gels.It is expected to provide a reference …

Mechanisms Of Antioxidant Activity Of Lactic Acid Bacteria And Its Application In Oxidative Stress-Related Diseases, Yixiao Shao, Chuang Huang, Chenjian Liu, Xiaoran Li

Journal of Food Science and Biotechnology

The antioxidant properties of lactic acid bacteria are related to various probiotic activities,which can prevent and alleviate a variety of chronic diseases caused by oxidation in the host.The antioxidant mechanisms of lactic acid bacteria,including the action mechanisms of antioxidant enzymes and non-enzymatic antioxidants and regulation of host signaling pathways,were expounded.Furthermore,the application status of antioxidant properties of lactic acid bacteria in diseases of the nervous system,digestive system,and skin system were summarized.The aim is to explore how to enhance the antioxidant activity of lactic acid bacteria in oxidative stress-related diseases and give new insights into the development of related treatment strategies.

Reconstruction And Analysis Of Genome-Scale Metabolic Model Of Aspergillus Oryzae Fmme-S- 38, Cong Gao, Weijia Yuan, Chao Ye, Jia Liu, Liming Liu

Journal of Food Science and Biotechnology

[Objective ] A genome-scale metabolic model (GSMM ) of the high L-malic acid-producing strain Aspergillus oryzae (A.oryzae ) FMME-S- 38 was constructed and analyzed.[Method ] The new GSMM iJW1333 was established for A.oryzae FMME-S- 38 through a two-step process of draft reconstruction and model refinement.[Result ] The model integrated 1 723 biochemical reactions,1 723 metabolites,and 1 333 functional genes,achieving the prediction accuracy of 94.6% for substrate metabolism and 92.0% for cell growth.Comparative genomic analysis revealed that mutations in the genes involved in the hexose monophosphate pathway (HMP ) and oxidative phosphorylation underpinned the enhanced L-malic acid production of A.oryzae FMME-S- …

Effects Of Extraction Methods On The Composition And Immunological Activities Of Yeast Mannoproteins, Haibo Zhang, Yongjun Xia, Guangqiang Wang, Ning Peng, Zhixian Chen, Lianzhong Ai

Journal of Food Science and Biotechnology

[Objective ] The extraction efficiency of different methods and the effects of these methods on the composition of mannoproteins extracted from yeast cell walls were compared.Furthermore,the anti-inflammatory activities of the extracted mannoprotein were evaluated.[Method ] Three yeast mannoproteins (MP-W,MP-B,and MP-E ) were extracted via high-temperature water,alkali,and enzyme-assisted extraction methods and purified via ethanol precipitation.The identification of monosaccharide composition and amino acid composition,distribution of relative molecular mass,and Fourier transform infrared spectroscopy were employed to analyze the composition and chemical structures of yeast mannoproteins.A cell experiment was carried out to examine the anti-inflammatory activity.[Result ] The yields of yeast mannoproteins extracted by …

In Vitro Antioxidant And Anti-Inflammatory Activities Of Tea Flower Extract And Its Inhibitory Effect On Cell Aging, Shishuai Yu, Yining Hao, Bo Peng, Youyun Liu, Liping Qu

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the in vitro antioxidant and anti-inflammatory activities of Yunnan tea flower extract (TFE ) and its Inhibitory effect on cell aging.[Method ] The main active components in TFE were determined by ultraviolet (UV)-visible spectrophotometry and high performance liquid chromatography (HPLC ).The antioxidant and anti-inflammatory activities of TFE were determined by the free radical scavenging assay and UV-induced photoaging model of human keratinocytes (HaCaT ),respectively.After the aging model of fibroblasts was established,the aging level of cells was assessed by the β-galactosidase (β-gal) staining assay,and the expression of matrix metalloproteinase- 1 (MMP- 1),matrix metalloproteinase- 3 …

Synthesis Of N-Acetyl- Trans -4-Hydroxyproline Catalyzed By Acyltransferase, Chuanxiang Wei, Keru Chen, Yun Hu, Chao Sun, Rukang Xue, Fubao Sun, Jingfang Cheng

Journal of Food Science and Biotechnology

[Objective ] This study aims to establish the biosynthesis process of N-acetyl- trans -4-hydroxyproline (N-ATHOP ).[Method ] Escherichia coli (E.coli ) BL21 (DE3) was selected as the host to heterologously express the acyltransferase (MsAcT ) derived from Mycobacterium smegmatis.A semi-rational design was employed to construct the mutant MsAcT (S11C) capable of catalyzing the synthesis of N-ATHOP.[Result ] The catalytic reaction conditions of the mutant MsAcT (S11C) were optimized as pH 6.9,reaction temperature 49 ℃,initial trans -4-hydroxyproline (THOP ) concentration of 315.5 mmol/L,and vinyl acetate volume fraction of 10%.Under the optimized conditions,the yield of N-ATHOP synthesized under the catalysis of MsAcT …

Zeaxanthin Encapsulated With High Internal Phase Emulsions And Its Stability, Ya Li, Ying Ou, Jie Lu, Shuo Meng, Shoushuai Feng, Hailin Yang

Journal of Food Science and Biotechnology

[Objective ] This study prepared highly stable high internal phase emulsions (HIPEs ) to encapsulate zeaxanthin,thereby improving the stability and expanding the food and pharmaceutical applications of zeaxanthin.[Method ] HIPEs were prepared with the gelatin-chitosan suspension for encapsulation of zeaxanthin extracted from goji berries.Ultra-performance convergence chromatography (UPCC ) was employed to separate zeaxanthin.Confocal laser scanning microscopy (CLSM ) was employed to observe the emulsion structure.The rheological analysis was performed to characterize the emulsion,which was combined with the hydrogen peroxide and storage stability tests to evaluate the performance of encapsulation with HIPEs in improving the stability of zeaxanthin.[Result ] The separation …

Health Risk Assessment Of Multiple Mycotoxins In Rice , Maize , Soybean , And Wheat Flour Marketed In Shanghai, Zuoyin Zhu, Jie Wang, Junmei Ma, Hanke Zhao, Haisheng Cheng, Chengbao Huang, Junhua Yang

Journal of Food Science and Biotechnology

[Objective ] This study aims to evaluate the potential health risks to consumers from exposure to multiple mycotoxins in grains.[Method ] Point and probabilistic assessment models were adopted to analyze the dietary exposure risks of adult residents in Shanghai to aflatoxins (AFs),ochratoxin A (OTA ),zearalenone (ZEN ),deoxynivalenols (DONs ),and fumonisins (FBs) through consumption of rice,maize,soybeans,and wheat flour.[Result ] The margin of exposure values (MoE ) for AFs in maize and aflatoxin G 1 (AFG 1) in soybeans exceeded 10 000,indicating no dietary exposure risk.In contrast,AFs in rice and wheat flour,as well as aflatoxin B 1 (AFB 1),aflatoxin B 2 (AFB …

Identification And Spoilage Capacity Analysis Of Main Spoilage Bacteria In Pickles, Li Zhu, Huimin Li, Liejiang Yuan, Cancan Peng, Bo Zhou, Sheng Song, Haizhi Wu, Mingyang Zhu, Fangmei Wang, Yu Liu, Yuqian Hu, Ge Li, Yiqing Sun, Yi Zhou

Journal of Food Science and Biotechnology

[Objective ] This study aims to identify the main spoilage bacteria and evaluate their spoilage capacity in the spoilage process of pickles.[Method ] High-throughput sequencing was employed to analyze the microbial community dynamics in the samples of pickles at the early,middle,and late stages of spoilage.The key spoilage bacteria were isolated,purified,and then identified through a combination of colony morphology observation,biochemical analysis,and 16S rDNA/ITS sequencing.Re-inoculation experiments were carried out for further validation of the primary spoilage bacteria in pickles.[Result ] Acinetobacter and Bacillus were the dominant spoilage bacterial genera across the three spoilage stages.Notably,Bacillus exhibited rapid growth over time,with the relative abundance …

Establishment And Application Of An Immunomagnetic Bead-Based Melisa Method For Olaquindox, Ziyi Chen, Yanling Song, Junjie Sun, Yinlai Li, Lu Zhai, Yaofeng Zhang, Junli Feng, Renyao Jin

Journal of Food Science and Biotechnology

[Objective ] This study aims to establish a magnetic bead enzyme-linked immunosorbent assay (MELISA ) method for olaquindox (OLA ) based on immunomagnetic beads.[Method ] First,olaquindox-hapten conjugated with horseradish peroxidase (OLA-HS-HRP ) and immunomagnetic beads were synthesized.Then,the conjugated immunomagnetic beads and OLA-HS-HRP were used to screen and optimize the reaction conditions of MELISA.[Result ] The optimal working dilution factors for the immunomagnetic beads and OLA-HS-HRP were 100 times and 8 000 times,respectively,with the antibody dilution buffer being PBS solution (0.010 mol/L,pH 7.0) containing methanol at a volume fraction of 5%.Under the optimized conditions,the established standard equation was y=13.593ln x+17.173 (R2=0.994 …

Establishment And Application Of A Uplc-Ms/Ms Method For Simultaneous Determination Of 39 Veterinary Drugs And Their Metabolites In Black Bone Chicken, Jiawei Du, Yongqin Li, Shuangyuan Jin, Xueyan Wang, Hongwu Wen, Xuelian Guo, Hao Li, Lihua Xu

Journal of Food Science and Biotechnology

[Objective ] This study aims to comprehensively investigate the risk of veterinary drug residues in black bone chicken.[Method ] A method for rapid and simultaneous analysis of 39 veterinary drugs and their metabolites in three categories was established based on low-temperature ultrasonic extraction,solid-phase extraction pretreatment,and ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS ).The samples were extracted by Mcllvaine-Na 2EDTA and phosphate buffer via ultrasonic treatment at low temperatures,purified by Oasis HLB solid-phase extraction column,separated by T 3 chromatographic column,and quantified by the matrix matching external standard method.[Result ] The 39 veterinary drugs and their metabolites showed good linear relationships in …

Serum Metabolomic Profiling Of Incident Type 2 Diabetes Mellitus In The Multi-Ethnic Study Of Atherosclerosis And Rotterdam Study, Xuanwei Jiang, Fang Zhu, Gonçalo Graça, Xihao Du, Jinjun Ran, Fariba Ahmadizar, Alexis C Wood, Yanqiu Zhou, Denise M Scholtens, Ali Farzaneh, M Arfan Ikram, Alan Kuang, Carel W Le Roux, Meghana D Gadgil, Marilyn C Cornelis, Kent D Taylor, Xiuqing Guo, Mohsen Ghanbari, Laura J Rasmussen-Torvik, Russell P Tracy, Alain G Bertoni, Jerome I Rotter, David M Herrington, Philip Greenland, Maryam Kavousi, Victor W Zhong

Children’s Nutrition Research Center Staff Publications

Objective: This study aimed to investigate serum metabolomic biomarkers associated with incident type 2 diabetes mellitus (T2DM) and evaluate their performance in improving T2DM risk prediction.

Methods: Untargeted proton nuclear magnetic resonance (1H NMR) spectroscopy-based metabolomics analyses were conducted in the Multi-Ethnic Study of Atherosclerosis (MESA; n = 3460; discovery cohort) and The Rotterdam Study (RS; n = 1556; replication cohort). Multivariable cause-specific hazards models were used to analyze the associations between 23 571 serum metabolomic spectral variables and incident T2DM. Replicated metabolites required an false discovery rate-adjusted P < .01 in MESA, P < .05 in RS, and consistent direction of association. Pathway and network analyses were conducted to elucidate biological mechanisms underlying T2DM development. The utility of the replicated metabolites in improving T2DM risk prediction was assessed based on the Framingham Diabetes Risk Score. A 2-sample Mendelian randomization was conducted to assess causal associations.

Results: Nineteen metabolites were significantly associated with incident T2DM. Pathway analyses …