Digital Commons Network^™

Relationship Between Silage Quality And 15 N-Nitrate Reduction During Ensilage, K Ataku, M Horiguchi, T Matsumoto

IGC Proceedings (1977-2023)

Nitrate in forage has been proven to occasionally poison ruminants. Our recent studies, however, have shown that a high con­centration of nitrate inhibits butyric acid fermentation to the advantage of the silage quality. This work was undertaken to see the relationship between silage quality and the pattern of nitrate reduction during ensilage.

Oregon State Rank Assessment For Willamette Navarretia (Navarretia Willamettensis), Nora Dunkirk

Institute for Natural Resources Publications

Oregon state conservation status assessment for Willamette Navarretia (Navarretia willamettensis) using NatureServe methodology, 2026.

Lesson 3. Sky Explorers: The Rhythm Of The Moon And My Garden, Rachel Pastrana, Madhav P. Nepal

iLEARN Teaching Resources

In this kindergarten lesson, students become "Sky Explorers" as they investigate the patterns and rhythms of the Sun, Moon, and Earth. Through storytelling, movement activities, Oreo Moon modeling, outdoor observations, and hands-on exploration, students learn how Earth's rotation creates day and night and how the Moon changes appearance through predictable phases. Students explore the Lakota concepts of Wí (Sun), Haŋwí (Moon), and Mitákuye Oyás'iŋ (We Are All Related) while making connections between natural cycles, food systems, and plant growth. Students discover how people have long observed the Moon as a natural calendar and how farmers and gardeners use seasonal patterns …

Examining The Relationship Between Civic Agriculture Participation And Civic Engagement In South Texas, Allison Kaika, Dongkyu Kim, William Donner, Alexis Racelis, Owen Temby

School of Earth, Environmental, & Marine Sciences Faculty Publications

Civic agriculture is often credited with strengthening democratic life by encouraging civic and political engagement, yet this claim has scarcely been tested in socioeconomically marginalized regions. Using original survey data collected through a combination of random and purposive sampling, this study assesses that relationship in the Lower Rio Grande Valley of Texas, a majority-Hispanic agricultural region marked by high poverty and low civic participation. Phone and online surveys were administered in English and Spanish to reach both the general population and individuals involved in civic agriculture initiatives, yielding over 400 responses. We examine whether civic agriculture participation predicts greater community …

Lesson 2. The Soil Doctors: Healthy Vs. Thirsty Soil Detectives, Rachel Pastrana, Madhav P. Nepal

iLEARN Teaching Resources

In this lesson, Kindergarten students in Norris, South Dakota, become "Soil Detectives" to investigate the health of Maka (the Earth). By using their hands to touch, squeeze, and compare local schoolyard soil, students learn to identify Maka Wasté (Healthy Soil) and Maka Puza (Thirsty Soil). This lesson utilizes communal storytelling and Lakota terminology to ground scientific inquiry in local Indigenous knowledge and South Dakota land history. Students apply Regenerative Agriculture principles by providing a "cure" protecting the soil with a natural blanket—and conclude with a Wóglaka (Reflection Circle) to share their experiences as young stewards of the land.

Proteomic Evidence Indicates Ultrasound-Assisted Extraction Enhances Recovery Of Membrane-Associated Proteins In Hericium Erinaceus, Tanvir Ahmed, Angéla Juhász, Utpal Bose, Netsanet Shiferaw Terefe, Michelle L. Colgrave

Research outputs 2022 to 2026

Membrane-associated fungal proteins mediate essential functions and contain bioactive proteins with therapeutic potential but are underrepresented in aqueous extracts due to hydrophobicity and lipid associations. Ultrasound-assisted aqueous extraction was applied to Lion’s mane (Hericium erinaceus), and the proteins were fractionated by molecular weight (≥30 kDa, 10–30 kDa, 3–10 kDa). Proteomic profiling using data-dependent (DDA) and data-independent (DIA) mass spectrometry identified 1500 and 1702 proteins, respectively (1% global FDR). Ultrasonication induced surface irregularity and a less compact, heterogeneous microstructure. Topology predictions showed higher proportions of transmembrane and secretory proteins in ultrasonicated fractions. Extraction preserved protein integrity (82% molecular-weight retention, 97% tryptic …

Agrivoltaic Shading Reduces Soil Greenhouse Gas Fluxes In A Semi-Arid Vineyard, Marcelo Gomes Da Silva, Rafael M. Almeida, Carine Cocco, Engil Pereira

School of Earth, Environmental, & Marine Sciences Faculty Publications

Agrivoltaic systems are expanding in semi-arid regions, raising questions about how panel driven shading alters soil greenhouse gas exchange. We used automated chambers to measure high frequency soil CO2, N2O, and CH4 fluxes in a newly established vineyard agrivoltaic system in Texas across three shading intensities (0%, 35%, 88%) that represent the light environment beneath panels as shaped by panel tilt and solar trajectory. Shading primarily cooled the soil, reducing mean soil temperature from 35.9 °C (0%) to 32.5 °C (35%) and 30.5 °C (88%), while mean soil water content shifted modestly (0.125, 0.106, and 0.130 m3 m−3, respectively). Over …

Impact Of Dolutegravir-Based Regimens On Patient Satisfaction And Quality Of Life Among People Living With Hiv: A Systematic Review And Meta-Analysis, Mustapha Muhammed Abubakar, Christabel Ogechukwu Okoye, Ruth Jeminiwa, Ifeanyi Kanayo Ifeorah, Lotanna Ezeja, Yusuf Dauran, Sahabi Musa, Silas Usen Joel, Abdurrahman Murtala Bello, Amauche Pearl Ngige, Gabriel Ezenri, Abdulmuminu Isah, Ifunanya Ikhile, Blessing Onyinye Ukoha-Kalu

College of Pharmacy Faculty Papers

Background: Despite the global scale up of dolutegravir (DTG)-based regimens as the preferred first-line therapy for persons living with HIV (PLHIV), no comprehensive evidence exists on its effect on patient-reported outcomes such as Quality of Life (QoL) and patient satisfaction (PS). We conducted a systematic review and meta-analysis to provide the first comprehensive quantitative synthesis of the effect of DTG-based regimens on QoL and PS among PLHIV.

Method: We conducted literature search on PubMed, Scopus, Embase and Cochrane Library databases for peer-reviewed, primary studies published from January 2015 to January 2025. Studies using validated tools to evaluate QoL and PS …

Intramuscular Nad⁺ Therapy And Neuroenergetic Symptom Modulation: An Exploratory Retrospective Human Pilot Study, Zachary J. Dickey Do, Kirtan Patel Bs, Randy Aldret Edd, Cscs, Atc, Navneet Sharma Md, Glenn Goodwin Do

Journal for Sports Neuroscience

Abstract

Background: Nicotinamide adenine dinucleotide (NAD⁺) is a central regulator of neuronal energy metabolism, mitochondrial function, and axonal integrity. Following mild traumatic brain injury (mTBI) and repetitive subconcussive exposure, neurons undergo a neurometabolic crisis characterized by impaired oxidative phosphorylation, increased ATP demand, and depletion of intracellular NAD⁺ pools. Pre clinical models demonstrate that NAD⁺ depletion contributes to central energy dysregulation, cognitive dysfunction, and axonal and neuronal degeneration through disruption of NAD⁺-dependent pathways including SIRT1-mediated mitochondrial resilience, PARP1-driven NAD⁺ consumption, and SARM1-associated axonal breakdown. Despite growing preclinical evidence supporting NAD⁺based neuroprotection, human clinical data remains absent.

Methods: A real-world clinical pilot …

Rape Pregnancies And Consequent Ptsd Resulting From The Overturn Of Roe V. Wade, Emma Lindenfelser, Yulia Garaeva, Nenette Nti-Agyemang, Ellen Davis, Anna Kabwa, Alexandra M Calaman

Binghamton University Undergraduate Journal

Prior to the 1973 Roe v. Wade ruling, access to safe legal abortions was limited and often restricted along racial, economic, and geographic lines. Nearly fifty years later, women’s health policy evolved again following the 2022 Dobbs v. Jackson Women’s Health Organization decision overturning Roe v. Wade. This court ruling decimated federal protections of abortion. This paper presents the research that the 2022 Dobbs judicial decision may contribute to and exacerbate rape-related PTSD on 1 out of 20 American who are unable to access a safe abortion after a rape-related pregnancy. Pregnant women living in conservative states that have outlawed …

Chromatin Insulators Homie And Nhomie Can Interact With Distant Copies Either Together Or Separately, With Distinct Outcomes For Enhancer-Promoter Interactions, Miki Fujioka, Wenfan Ke, Paul Schedl, James B. Jaynes

Department of Biochemistry and Molecular Biology Faculty Papers

Chromatin insulators, a.k.a. boundary elements, separate regions of the chromosome with distinct chromatin characteristics, including distinct histone modifications. This activity affects gene expression by allowing chromatin domains to be stably regulated and maintained. Insulators also block enhancer-promoter interactions and, somewhat paradoxically, facilitate other interactions, particularly when they stitch together distant regions of the chromosome by pairing with specific partners. Here we explore how long-range interactions facilitated by insulator pairing are affected by the presence of two potentially competing partners. Our results show that when two partners are present, they can reduce each other's effects on distant gene expression, suggesting that …

Disease-Causing Mfn2 Mutants Impair Mitochondrial Fission Dynamics By Distinct Drp1 Dysregulation, Daniel Lagos, Pamela R. De Santiago, Nicolás Pérez-Bravo, Benjamín Cartes-Saavedra, Josefa Vial-Brizzi, Diego Troncoso-Chandía, Oliver Podmanicky, Rita Horvath, Verónica Eisner

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Mitochondria undergo fusion and fission. While DRP1 regulates fission, fusion is controlled by OPA1, MFN1, and MFN2. The balance between these processes and the crosstalk between machineries remains poorly understood. MFN2 mutations cause Charcot-Marie-Tooth disease type 2 A (CMT2A), affecting mitochondrial fusion and morphology. However, their role in fission is unclear. Using skin fibroblasts from CMT2A patients (L248H and M376V MFN2 mutations) and wild-type mouse embryonic fibroblasts expressing these variants, we studied how MFN2 mutations impact mitochondrial dynamics beyond fusion. We analyzed mitochondrial morphology and dynamics by live-cell confocal microscopy and tested fusion/fission protein levels, oxygen consumption rate (OCR), extracellular …

Discovery Of Bmi-Associated Gut Microbiome Signatures In The Awi-Gen 2 Cohort, Meriem Yagoubi

Theses and Dissertations

Obesity is increasing across sub-Saharan Africa amid rapid dietary, environmental, and urbanization transitions, yet the gut microbiome features associated with adiposity in African populations remain under-characterized. This study investigated whether species-level gut microbiome composition is associated with body mass index (BMI) in the AWI-Gen 2 cohort, and whether BMI-associated microbial signals remain detectable after accounting for the dominant geographic structure of the microbiome. Gut microbiome community structure and diversity were characterized across 1,665 participants, and differential abundance analysis was used to identify taxa associated with BMI after controlling for study site. Machine learning models were then trained to assess how …

Synthesis And Characterization Of Synergistic Bioactive– Organometallic Condensation Polymers, Francesca Isabel Mosca

Electronic Theses and Dissertations 2020 - Present

This research focuses on the synthesis and structural characterization of polymers with anticipated anticancer and antiviral properties by combining metal-containing Lewis acids, organotin dichlorides (R2SnCl2), Group 4 metallocene dichlorides (Cp2TiCl2, Cp2ZrCl2 and Cp2HfCl2), and triphenyl Group 15 metal dichlorides (Ph3AsCl2, Ph3SbCl2 and Ph3BiCl2), with biologically active Lewis bases, lamivudine, a synthetic nucleoside, and camphoric acid, a dicarboxylic acid, to achieve synergistic effect.

Five classes of polymers were synthesized using interfacial polymerization: lamivudine and organotin dichlorides, lamivudine and Group 4 metallocene dichlorides, camphoric acid and triphenyl Group 15 metal dichlorides, camphoric acid and organotin dichlorides, and camphoric acid and Group 4 …

Synthesis And Characterization Of Organometallic Polymers And Their Cytotoxicity Towards Human Cancer Cell Lines, Paul Peter Slawek

Electronic Theses and Dissertations 2020 - Present

This research aimed to synthesize organometallic polymers from dialkyl (methyl, ethyl, butyl, cyclohexyl, and octyl) and diaryl (phenyl) tin dichlorides and three organic monomers, dipicolinic acid (DPA), chloramphenicol (CH), and isomannide (IS), using interfacial polymerization. Structure-activity relationships were investigated through physicochemical characterization (light-scattering photometry, proton NMR, and IR spectroscopy) and cytotoxicity was evaluated against six human cancer cell lines (AsPC-1, PANC-1, MCF-7, MDA-MB-231, HT-29, PC-3), with cisplatin serving as the reference standard.

Five DPA/tin polymers were synthesized, with IC₅₀ values in the range of 0.60–0.71 μg/mL. Their cytotoxicity was significantly lower than that of cisplatin (0.0023-0.044 μg/mL), suggesting that …

Comparison Of Fish Biomass And Fish Carbon Content Associated With Reef Sites At The Rio Grande Valley Artificial Reef In The Gulf Of Mexico, Allison K. White, Md Saydur Rahman, Richard J. Kline

School of Earth, Environmental, & Marine Sciences Faculty Publications

As global fisheries stocks have decreased due to overfishing and climate change, artificial reefs have gained significant attention. In addition to providing or restoring habitat, artificial reefs may serve as carbon capture sinks and potential climate mitigation strategies. The Rio Grande Valley (RGV) Reef is an artificial reef located in the northwestern Gulf of Mexico off the coast of Texas. The RGV Reef area spans 1,650 acres and is comprised of hundreds of groupings of recycled and pre-formed materials ranging from low relief (1–2 ft) to high relief (8–30 ft) reef structures. This reef provides a variety of novel habitat …

Growing Pains: The Loss Of Plasticity To Extreme Anoxia In Developing Embryos Of Austrofundulus Limnaeus, Patrick Ryan Clouser

Dissertations and Theses

Oxygen plays a pivotal role for most animal life on Earth, so much so that even short exposures to no oxygen, anoxia, can have severe effects on the animal's wellbeing. Conversely, aerobic recovery after anoxic insult can be just as detrimental. Some animals are the exception to this rule and demonstrate survival through anoxia and recovery. There has been a great deal of research around anoxia tolerant animals, but what remains unclear are the genetic underpinnings that determine the anoxia tolerant phenotype. RNA sequencing allows for the quantification of RNA transcripts within a sample, which can indicate if a gene …

Biologically Informed Negative Samplingfor Antibody Chain Pairing Classification, Ishita Singh

Computer Science Senior Theses

Antibody heavy and light chain (H/L) pairing is fundamental to antigen recognition and stability. While single-cell sequencing preserves native pairing information, widely used bulk repertoire and spatial transcriptomics platforms do not, motivating the need for efficient ML methods to infer H/L pairing. Training a binary classifier for this task faces the methodological challenge of a lack of true biological negatives, since natural selection eliminates B cells with incompatible H/L pairs.

In this thesis, I introduce a biologically informed negative sampling strategy for H/L pairing classification, drawing on known V-gene biases in heavy and light chain pairing. Pseudo-negatives are constructed by …

Wrack Lines Spring-Summer 2026 Volume Number 1, Margaret Cozens, Johann Heupel, Owen Placido, Paul Salopek, Judy Benson

Wrack Lines

“Building community around the places we love,” is the theme of the Spring-Summer 2026 issue of Wrack Lines magazine. Learn about the work of the Six Lakes Park coalition in Hamden, Riverfront Recapture in Hartford, MyCoast in southeastern Connecticut and the statewide land conservation conference. Additional stories tell about the artistry and mission of photographer-videographer Tomas Koeck, and National Geographic explorer Paul Salopek, who is traveling the world on foot.

National Agricultural Library (Nal) Search., Leah Everitt, Sonya Lemke

Publications

SEARCH is the USDA National Agricultural Library (NAL)'s main discovery platform that provides integrated access to the NAL Digital Collections, Special Collections, PubAg and AGRICOLA. NAL SEARCH is a valuable tool for health science librarians, students, and professionals seeking veterinary science, nutrition, or animal experiment alternatives. This article aims to give users a comprehensive overview of this resource.

Diversity, Systematics, And Phylogenomics Of The Neotropical Glass And Longtail Knifefishes (Gymnotiformes: Sternopygidae), Kevin Thomas Torgersen

Doctoral Dissertations

This dissertation provides a comprehensive systematic and phylogenetic study of the electric knifefish family Sternopygidae, integrating morphological, molecular, osteological, and phylogenomic data to clarify species diversity, evolutionary relationships, and biogeographic patterns in this Neotropical group. Across six studies, the work reveals that Sternopygidae diversity has been significantly underestimated due to morphological conservatism that obscures species boundaries. Chapter 1 revises the genus Sternopygus in the Guiana Shield, identifying nine species (five new) using osteology, morphometrics, and µCT imaging. Distinct anatomical traits and distribution patterns show strong regional structuring, highlighting the Guiana Shield as a diversification center. Chapter 2 describes a new …

Effects Of L-Baiba Supplementation With And Without Grains Of Paradise On Changes In Resting Metabolic Rate, Body Composition, And Cardiometabolic Risk Factors, Leah E. Allen, Paige J. Sutton, Alex Schrautemeier, Kevin Holley, Connor J. Gaige, Joesi M. Krieger, Anthony M. Hagele, Karenina Antequera Gutierrez, Petey Mumford, Chad Kerksick

Faculty Symposium

To evaluate the impact of 56 days of supplementing with L-BAIBA or L-BAIBA + GOP (BGOP) on resting metabolic rate, body composition, perceptual indicators of mood and affect, and cardiometabolic markers in overweight and obese men and women.

METHODS: Healthy men and women (43.2±11.4 years, 169.4±7.6 cm, 90.3±15.6 kg, 31.4±4.4 kg∙m-2) completed a weekly exercise program and followed a calorierestrictive diet and were randomly assigned in a double-blind and blocked manner according to sex and DXA lean mass to consume daily either a placebo (PLA) (n=12 [11F, 1M]), 500 mg L-BAIBA (L-BAIBA: MitoBurn™, NNB Nutrition) (n=13 [9F, 4M]), or …

First Course Portfolio For Plpt 803 Fundamentals Of Scientific Discovery, Hernan Garcia-Ruiz

UNL Faculty Course Portfolios

PLPT 803 Fundamentals of Scientific discovery is a new curse in the Department of Plant Pathology at the University of Nebraska-Lincoln. I designed and developed this course from the ground up. Initially, it was offered as a special topic (PLPT 892-014) for four years. PLPT 803 was approved by the College of Agricultural Sciences and Natural Resources curriculum committee in September of 2025. PLPT 803 is unique within the University of Nebraska system and will be offered for the first time in the fall of 2027. The course is designed for students to learn fundamental scientific skills such as scrutinize, …

Life-Stage Specific Predatory Efficiency Of 2 Generalist Predators Of Bemisia Tabaci (Hemiptera: Aleyrodidae), Itohan Aigbedion-Atalor, Abdelmutalab G. Azrag, Alvin M. Simmons, Jason M. Schmidt

All Peer-Reviewed Publications

Bemisia tabaci (Gennadius) is an economically damaging invasive pest to global vegetable production. Current management strategies rely heavily on insecticides, but increasing levels of resistance raise sustainability concerns. Therefore, strengthening integrated pest management (IPM) programs requires enhancing biocontrol through improved predator-pest interactions. Despite the well-established relevance of functional response modulation by multiple environmental factors, limited research has explored how plant traits and prey life stages interact to influence the efficiency of generalist predators in vegetable systems. To address this gap, we evaluated the functional responses of 2 adult generalist predators, Hippodamia convergens (Guérin-Méneville) and Geocoris punctipes (Say), against 3 nymphal …

[2026 Winner] Seed Sovereignty Through The Lens Of Decolonization, Lily A. Marx

Ethnic Studies Research Paper Award

This paper explores the movement of seed sovereignty, a contemporary movement stemming from the history of theft, appropriation, and forced assimilation endured by indigenous peoples. This movement recognizes that consumers alone cannot mend the damage being done to our food systems, but instead encourages communities to take political action by saving seeds and rebuilding connections with the land.

Independent And Combined Effects Of Early-Life Iron Deficiency And Antibiotic Exposure On Growth And Hematological Outcomes In A Piglet Model, Ginger M. Fitzpatrick

Master's Theses

Iron deficiency (ID) affects over 2 billion individuals globally. Children under 5 are at increased risk of ID due to rapid growth and are especially vulnerable to its negative consequences because of iron’s essential role in cognitive development and immune maturation. ID is the leading cause of iron deficiency anemia (IDA), with IDA affecting approximately 40% of children in this age group. Antibiotic (abx) exposure is also prevalent during early life and may disrupt growth and through gut dysbiosis, with increasing use posing additional risk in younger populations. Geographically, there is overlap in regions with high anemia prevalence and those …

Exploring Regenerative Vegetable Systems On The Central Coast Of California, Una S. O'Connell

Master's Theses

As climate change and current agricultural practices place pressure on the long term sustainability and resilience of food production systems, there is growing interest in whether existing agricultural systems can adapt to maintain productivity while improving environmental outcomes.

This study evaluated the effects of regenerative versus standard organic vegetable production systems on yield, insect communities, and weed suppression across three field trials conducted on California's Central Coast. In addition, I collected baseline soil health data for all trials. Trials 1 and 2 were carried out at the Cal Poly San Luis Obispo Organic Farm using cabbage and broccoli as cash …

Sustainable Management Of Soilborne Pathogens In Strawberry Production Through Host Resistance And Sanitation Practices, Maria G. Alvarez Arredondo

Master's Theses

Strawberry production in California is significantly impacted by soilborne pathogens that reduce plant productivity and increase management costs. This research addressed management of these pathogens through two independent approaches: evaluation of host resistance in strawberry cultivars and assessment of sanitation practices for reducing pathogen movement between fields.

Macrophomina root rot, caused by Macrophomina phaseolina, was evaluated over two growing seasons (2024–2025 and 2025–2026) using field trials at California Polytechnic State University, San Luis Obispo. In Year 1, disease incidence varied widely among genotypes, with mortality ranging from 1.3% to 88.8% and an average mortality of 26.4%. Area under the …

Detection And Evaluation Of Hazardous Mercury Contamination In Various Biological Samples, Krish Italiya

Biochemistry and Molecular Biology

Mercury is a toxic environmental pollutant that threatens ecosystems and human health. This study investigates mercury contamination in Lactuca sativa (lettuce) and selected fish species, including tuna and salmon, focusing on plant responses and the limitations of X-ray fluorescence (XRF) analysis. In a controlled experiment, lettuce plants were exposed to mercury to evaluate uptake and toxicity, and XRF spectroscopy was used for quick, non-destructive elemental analysis. Results confirmed mercury accumulation in lettuce, along with visible stress symptoms such as leaf yellowing, purple pigmentation, and tissue damage. Mercury was not detected in tuna and salmon samples, likely due to concentrations below …

Updates To The Caniform Fauna (Carnivora, Caniformia) Of The John Day Formation, Oregon, Usa, John D. Orcutt, Kevin C. Talbott, Michael J. Monroe, Nicholas A. Famoso

Biology Faculty Scholarship

The John Day Formation of central Oregon preserves a diversity of Oligo-Miocene caniform carnivorans, many of which were first described from the formation. However, many historically important specimens lack detailed locality or stratigraphic information, complicating any analysis of faunal change through time. Here, we describe 16 new specimens of caniforms from the John Day Formation, most with precise stratigraphic information. We report multiple specimens of the amphicyonids Daphoenus Leidy, 1853, Paradaphoenus cuspigerus (Cope, 1878), and Temnocyon altigenis (Cope, 1878), all previously known from the formation. We also report the first definitive occurrence of the hesperocyonine canid Osbornodon Wang, 1994 from …