Digital Commons Network^™

Determination Of Eight Fungicide Residues In Eggs By Solid-Phase Extraction Coupled With Liquid Chromatography-Tandem Mass Spectrometry, Liang Jun, Chen Xiong, Wang Yu, Qu Zihao, Zhang Wei, Li Qing

Food and Machinery

［Objective］ To establish an analytical method for the determination of residues of eight fungicides ametoctradin, triadimefon,triadimenol, flutriafol, mandipropamid, penthiopyrad, isopyrazam, and prochloraz ) in eggs by solid -phase extraction (SPE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS ).［Methods］ The samples are ultrasonically extracted with acetonitrile. The extracts are purified using ProElut DPC solid -phase extraction cartridges. The purified eluate is concentrated under reduced pressure and reconstituted with acetonitrile.Chromatographic separation is performed on a Kinetex C18 column (2.6 μm,2.1 mm ×100 mm ) with gradient elution using 0.1% formic acid in water and acetonitrile as the mobile phase. Detection is carried …

Detection Of Early Browning Inside Fragrant Pears Based On Time-Frequency Images Of Acoustic And Vibration Signals, Zhang Feng, Yu Junyang, Xiao Ping, Zhang Hui, Wang Kai

Food and Machinery

［Objective］ To explore the detection efficacy of models constructed by combining time-frequency image features of the fragrant pears in acoustic and vibration with machine learning algorithms.［Methods］ Acoustic and vibration signals are collected from fragrant pears exhibiting varying degrees of internal browning using a custom-built non-destructive detection device. Time-frequency images are transformed using the short-time Fourier transform (STFT),continuous wavelet transform (CWT), and the S-transform. Then, texture feature parameters in 15 time-frequency domains are extracted, respectively, from 3 types of time-frequency images using the Gray-Gradient Co-occurrence Matrix (GGCM). Additionally, correlation analysis is performed between these feature parameters and the fragrant pear browning …

Effects Of Thermal Processing Methods On Flavor Quality Of Fish Meat And Differential Formation Mechanisms : A Review, Zou Wanghua, Hou Xiaoyi, Liu Miao, Deng Na, Ni Panxianzhi, Li Hui, Fu Haohua, Wang Jianhui

Food and Machinery

Thermal processing is the most commonly used technique for fish products. Different thermal processing techniques vary in the degree of lipid oxidation, protein degradation, and Maillard reaction, as well as the synergy among these reactions, thereby differentially affecting flavor. Focusing on four typical thermal processing methods, steaming, boiling, frying, and roasting, this paper reviews the flavor change patterns in various types of fish products under different thermal processing conditions. Moreover, from the perspective of the synergy among lipid oxidation and decomposition, protein degradation, and the Maillard reaction, this paper systematically elucidates the formation mechanisms underlying the flavor substance enrichment and …

Advances In Extraction And Detection Technologies For Veterinary Drug Residues In Food, Luo Qiaoqiao, Yuan Yufeng, Jia Yunzhu, Chen Qingya, Tan Fang, Zhao Xin

Food and Machinery

Veterinary drug residues constitute a global challenge that poses significant threats to food safety and public health. This review systematically summarizes recent advances in detection technologies for veterinary drug residues in food, with a particular focus on the performance characteristics and application status of sample extraction techniques (including liquid-liquid extraction, solid phase extraction, accelerated solvent extraction, QuEChERS, and magnetic solid phase microextraction ),as well as analytical methods (including chromatographic-mass spectrometric techniques, immunoassays, capillary electrophoresis, biosensors, and quantum dots -based approaches ). Comparative analysis reveals that accelerated solvent extraction (ASE ) and QuEChERS have emerged as mainstream pretreatment techniques owing to …

Children With Cerebellar Malformations And Co-Occurring Autism: A Phenomenological Study Exploring Challenges Faced With The Dual Diagnosis Process, Service Acquisition, And Psychosocial Impacts, Ladeana W. Gambill

Doctoral Dissertations and Projects

Parents of children who have been diagnosed with cerebellar malformations, such as Dandy-Walker Malformation, often face confusion and frustration as they are given uncertain prognoses about their child’s future. The uncertainty is further compounded when there are concerns for autism spectrum disorder (ASD), and testing is sought, with conflicting answers on whether their child has autism or not. Acquisition of autism-specialty services may be delayed if a diagnosis of autism is not received in a timely manner. The purpose of this interpretive phenomenological study was to understand the lived experiences and challenges faced by parents of children with cerebellar malformations …

Diversifying Clinical Trial Participation: Leveraging Digital Health Innovations And Community Outreach For Recruitment And Retention Improvements, Blake Aaron Lambert

Doctoral Dissertations and Projects

The purpose of this qualitative study was to explore how digital tools and community outreach can improve recruitment and retention in clinical trials, particularly among underserved populations. This study examined key barriers to participation, the potential benefits and limitations of digital tools, and strategies to engage underrepresented groups. Surveys were conducted with healthcare professionals (HCPs) and community members to gather perspectives on participation in clinical research. NVivo was used to code and analyze data. The analysis identified themes related to cultural barriers and trust, awareness, logistical barriers and accessibility, structural barriers and relevance, digital tools, and engagement. Participants were expected …

The Effect Of Project-Based Learning Model On Entrepreneurship (Pjbl-E) With A Deep Learning Approach On Students' Problem-Solving And Cognitive Skills, Ricce Oktasari, Irwandi Irwandi, Siti Darwa Suryani

Jurnal Pendidikan Sains

This study aims to examine the effect of the Entrepreneurship-based Project Based Learning (PjBL-E) model integrated with a pedagogical Deep Learning approach on students’ problem-solving skills and cognitive learning outcomes in biology learning. The novelty of this study lies in the integration of entrepreneurship values and pedagogical deep learning principles into Project Based Learning to create contextual and meaningful learning experiences. Entrepreneurship integration encourages students to develop innovative products, identify real-world problems, and apply biological concepts in practical and socio-economic contexts. Meanwhile, the pedagogical Deep Learning approach promotes reflective thinking, conceptual understanding, critical analysis, and the ability to connect theory …

Student Worksheets Based On The Local Wisdom Of Besilek Serawai For Sixth-Grade Elementary Science Learning, Mice Agustin, Tomi Hidayat, Irwandi Irwandi

Jurnal Pendidikan Sains

This study aims to develop a Student Worksheet (Lembar Kerja Peserta Didik [LKPD]) based on the local wisdom of Besilek Serawai for sixth-grade elementary science education and to evaluate its validity. The study employed the ADDIE development model, consisting of five stages: Analyze, Design, Develop, Implement, and Evaluate. The developed LKPD integrates Besilek Serawai, a traditional martial art of the Seluma community, with the concept of the human locomotor system in science instruction. The worksheet was validated by two subject-matter experts and two media experts. Material validation yielded an average score of 4.26, equivalent to a validity percentage of 85.2%, …

Preoperative Low-Intensity Blood Flow Restriction Training Does Not Improve Quadriceps Strength Or Hypertrophy After Anterior Cruciate Ligament Reconstruction, Daniel Mcgurren, Kaycee Glattke, Molly Klanderman, Jedediah Lee, Anikar Chhabra, Arthur De Luigi

Journal for Sports Neuroscience

Objectives: To investigate the efficacy of a preoperative low-intensity blood flow restriction (LI-BFR) training intervention for patients undergoing anterior cruciate ligament reconstruction (ACLR).

Design: Pilot study

Methods: Twenty-eight patients undergoing primary ACLR participated in either a LI-BFR training or sham intervention at a rate of 2 sessions per week for 2 weeks before surgery. Quadriceps strength and cross-sectional area were measured via handheld dynamometry and leg press test before and after the intervention before surgery and at 5 different time points after surgery, through 24 weeks after surgery.

Results: Adjusting for sex, time, and strength/hypertrophy at baseline, there were no …

Dual-Action Antibacterial Agents Targeting Biofilm Reduction And Membrane Disruption In Staphylococcus Aureus, Rahmat Folashade Zakariyah, Waliyullahi Akorede Husain, Elohor Precious Samuel, Wonder Olanipekun Oloruntoba, Fatmah Opeyemi Saka, Khalid Ayinde Oyelami, Mariam Temitope Baker

Al-Bahir

Antimicrobial resistance in Staphylococcus aureus is a serious global health problem as the organism rapidly develops resistance against widely used antibiotics. This study sought to investigate the antibacterial and antibiofilm properties of γ-Tocopherol, a natural compound from Ocimum gratissimum, as well as its synergism with 5-hydroxymethylfurfural (5-HMF) against S. aureus. The antibacterial activities and minimum inhibitory concentrations of the compounds were determined using agar diffusion and microbroth dilution methods, respectively. Biofilm reduction was evaluated using 2,3,5-triphenyltetrazolium chloride (TTC) assay and membrane integrity was evaluated using the crystal violet microtiter plate assay and the ion leakage assay. The results …

A Rho5-Cnc1-Dnm1 Signalling Module Coordinates Multi-Organelle Execution Of Regulated Cell Death In Yeast, Justin Bauer

Theses and Dissertations

Regulated cell death is a conserved process with major implications for health and disease, yet how yeast cells integrate stress signals to determine survival versus death remains unclear. This thesis investigates Cyclin C (Cnc1), a conserved Mediator kinase module component with both transcriptional and mitochondrial roles. Together with Cdk8 Cnc1 primarily represses stress response genes, while upon stress it translocates to the mitochondria to induce mitochondrial fragmentation with the dynamin-related GTPase Dnm1. Using structure-guided separation-of-function mutants, I demonstrate that Cnc1-dependent death requires its mitochondrial function rather than its transcriptional role, and that its effector Dnm1 acts as a signaling node …

Cardiovascular Disease Subtypes And Alzheimer's Disease: Phenotypic And Genetic Associations In The Uk Biobank And All Of Us Research Program, Aili Toyli, Chen Zhao, Kuan Jui Su, Hui Shen, Hong Wen Deng, Qing Hui Chen, Qiuying Sha, Weihua Zhou

Michigan Tech Publications

BACKGROUND: Cardiovascular disease (CVD) and Alzheimer's disease (AD) are major public health concerns that share overlapping risk factors and potential mechanistic pathways. Although vascular contributions to cognitive decline are well documented, the specific relationships between AD and different CVD subtypes remain poorly understood. METHODS: In this cross-sectional study, we examined associations between AD and 11 CVD subtypes using logistic regression models in 2 large biobanks: the UK Biobank (n=502 133) and the All of Us Research Program (n=287 011). Models were adjusted for demographic, lifestyle, and clinical covariates. We also explored genetic overlap between AD and CVD traits through proximity-based …

Quantitative Determination Of Nano Delivered Kras Sirna, Axel S. Deraspe

USF Tampa Graduate Theses and Dissertations

This study focuses on the development and validation of analytical methods for the detection of therapeutic KRAS-targeting siRNA in biological systems. Physicochemical characterization showed that p5RHH-formulated siRNA nanoparticles exhibit a controlled nanoscale size distribution, moderate polydispersity, and a negative zeta potential, suggesting colloidal stability and potential suitability for cellular uptake. Scanning electron microscopy confirmed a predominantly spherical morphology with a relatively uniform size distribution, consistent with dynamic light scattering measurements.

To enable accurate siRNA quantification, stem–loop reverse transcription (SL-RT) coupled with SYBR Green–based quantitative Polymerase Chain Reaction (qPCR) was optimized. Key parameters, including a primer concentration of 100 nM and …

Antioxidant Potential Of A Non-Heme Iron Complex: Mitigating H2O2-Induced Cellular Damage, Magy A. Mekhail, David M. Freire, Cameron Bowers, Grant Elam, Nora Del Bosque, Hannah K. Pyle, Sarah K. Dunn, Spencer Lanyon, Timothy J. Hubin, Giridhar Akkaraju, Kayla N. Green

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Iron complexes with antioxidant activity have garnered significant interest for both general and medical applications, yet few studies have yielded functional nonheme iron-based mimics for H₂O₂ mitigation in aqueous environments. Here, we investigate water-soluble nonheme iron complexes derived from 12-membered pyridinophanes (^CF₃PyN₃, PyN₃, ^NMe₂PyN₃, and Py₂N₂) to evaluate the impact of ligand scaffolds on H₂O₂ decomposition activity both in vitro and in a cellular model. Speciation and kinetic analyses reveal that both electronic and geometric modulation of the macrocyclic …

Meningioma Detection From Mri: A Comparative Study Of Deep Learning Models, A. H. Doha, Sandi Jennifer, Fatima Alabdaly

AUIQ Complementary Biological System

This study compares several deep learning models including DenseNet20, CNN, ResNet50, VGG19, EfficientNet-B3, MobileNetV2, NASNetMobile, Xception to automatically classify brain meningiomas from MRI scans. A public dataset was used to evaluate the proposed model. All models were trained, validated, and tested on this dataset. Because meningiomas are common and manual reads, the goal is to speed and standardize diagnosis. Our main contribution is pairing a high-accuracy classifier with deep learning models. Among all models, DenseNet201 performed best, reaching 0.991% accuracy and reliably separating tumorous from non-tumorous MRIs. Future work will expand the dataset and incorporate stronger segmentation to further boost …

Impact On Energy Budgets In Grassland Production And Conservation Of Possible Production Of Energy From Biomass Crops, R J. Wilkins

IGC Proceedings (1977-2023)

There is commonly a conflict between output/unit of area of land and output/unit of support-energy (SE) input. The possibil­ity of using a proportion of the total land area for biomass energy production suggests an approach for calculating grassland out­put/ha unconfounded with differences in SE input. Output can be calculated for an area of land that is devoted to grassland and biomass energy crops in proportions that result in a net demand for SE of zero. Outputs of utilized metabolizable energy (UME) are related to inputs of SE for (1) grazed grass with high application of nitrogen (N) fertilizer and (2) …

Efficiency Of Dairying Under Contrasting Feeding And Management Systems In North America, Israel, Europe, And New Zealand, J.D. J. Scott

IGC Proceedings (1977-2023)

Four different systems of feeding and managing dairy cattle are seen in major milk-producing countries. These are (1) feeding purchased roughages and concentrates to zero-grazed, intensively penned cows; (2) home growing of roughages and most con­centrates for zero-grazed animals; (3) feeding concentrates with conserved pasture and other roughages in winter and reduced concentrates to grazed cattle in spring-summer; and (4) feeding cattle on grazed pasture and conserved pasture throughout the year. The following data on the four systems were obtained from examples in southern California, Wisconsin, England/Wales, and New Zealand. Average fat-corrected milk (FCM)/cow is approximately 7,140, 5,300, 4,670, and …

Potential Contribution Of White Clover To The Economics And Support-Energy Inputs Of Grass-Based Beef-Production Systems, T A. Stewart, R E. Haycock, A S. Laidlaw

IGC Proceedings (1977-2023)

Biological output, gross margins, and efficiency of support-energy usage in an 18-month beef-production system on grass-white clover swards receiving either 50 kg N/ha stocked at 3.3 head/ha (low N) or 300 kg N/ha stocked 4.5 head/ha (high N) were compared. To date, two production cycles (1977-1979 and 1978-1980) at two sites have been completed. Mean live-weight and carcass gains/ha were, respectively, 17.5% and 14% higher from the high-N than from the low-N system. Gross margin/head was higher from the high-N system in the first production cycle but not in the second. In the low-N system as com· pared with the …

An Evaluation Of The Black Walnut- Tall Fescue Pasture-Management System, H E. Garrett, W B. Kurtz

IGC Proceedings (1977-2023)

The economic and biological feasibility. of a black walnut-livestock-grazing multicropping-management regime bas been evaluated for comparison with a similar livestock-management regime conducted in the open. Pure tall fescue plots were established under open and black walnut-shade conditions. Forage yields on a dry-weight basis were estimated for a 3-year period. A concurrent grazing trial was co􀀌ducted on similar sites using 1.2-ha plots. Black walnut seedlings were planted within the plots on a 3 x 12-m spacing and protected from grazing animals by electric fencing. The economic analyses of the alternate grazing systems were based on their respective present net worth and …

Economics Of Renovating Mountain Hay Meadows, J J. Jacobs

IGC Proceedings (1977-2023)

There are approximately 0.5 million hectares (ha) of irrigated native grass hay in the mountain and intermountain regions of California, Oregon, Idaho, Utah, Nevada, Montana, Wyoming, and Colorado. Often these lands are not producing at their full physical or economic potential. The objectives of this study were to identify alternative improvement procedures to increase hay production and to determine the economic feasibility of those alternatives. The principal means of identifying alternative mountain hay-meadow improvement practices and their costs was personal in­terviews with ranchers. The ranchers interviewed were owners and/or operators of mountain ranches in Wyoming who were us­ing or had …

Pasture Demonstration Program For Beef: Economic Considerations, P R. Hasbargen, R H. Craven, N P. Martin

IGC Proceedings (1977-2023)

This study made an economic evaluation of several alternative pasture improvement programs on beef farms in northern Minnesota. Three questions were addressed relative to several pasture improvement systems. These were (1) Will it pay? (2) Is there a cheaper way? and (3) Is there a more profitable system for a particular farm resource situation? Partial budgeting and computerized linear programming techniques were used to analyze pasture cost and return data obtained from several ranches in northern Minnesota. Study results show that rented pastures, when available, provided the cheapest source of additional summer feed, and that legume pastures provide cheaper sources …

Pennsylvania's Alfalfa Growers Program: Four Years Of Success, J E. Baylor, L E. Lanyon, W K. Waters

IGC Proceedings (1977-2023)

Alfalfa (Medicago sativa L.) is the most important perennial forage legume grown in Pennsylvania. However, yields currently reported by farmers are considerably below what might be expected when all production inputs are utilized. Therefore, a pro­gram was developed (1) to measure how much alfalfa hay, protein, and energy can be pro􀀗uced on 1 ha of Pennsylvania land under nonirrigated conditions, (2) to determine the mineral content and uptake by high-yielding alfalfa crops, and (3) to obtain more realistic on-farm estimates of alfalfa production costs. To achieve the program goals, hay production for each harvest from 2.02-ha farm fields was …

Estimation Of Digestibility Of Legume Forages By Means Of Morphological Parameters, W Kuhbauch

IGC Proceedings (1977-2023)

Forage quality is normally evaluated by time-consuming and costly laboratory analyses. It appears promising to use mor­phological parameters to evaluate forage quality in alfalfa and red clover. During progressive growth of white clover, red clover, and alfalfa, the leaf and stem portion was separated to determine the fiber fraction according to the Van Soest method and to

estimate digestibility by the procedure of Tilley and Terry. Stem : leaf ratio and stand height as a measure of stem length were used as morphological parameters. Leaves of the three legumes vary only slightly in concentration of cell-wall material and in digestibility …

Income, Costs, And Profits Associated With Feeding Selected Forages To Dairy Cows, D D. Dornfeld, D A. Rohweder, W T. Howard

IGC Proceedings (1977-2023)

Over the last i0 years, dairy-farm enterprises have g:ro-wn in size, with an apparent increase in use of improved scientific feeding and management techniques. There in growing emphasis on automated feeding and milking and on management stcategies in an attempt to reduce labor needs and allow more cows/worker. The average herd size in the sample population was 76.8 cows. There was difficulty finding herds over 100 and under 40 head. It appears that the trend toward increasing growth will continue, as much as sufficient capital, management ability, and debt service capacity eJi:ist to permit the growth to occur. Forage management …

Economic Analysis Of The Impact Of Management Decisions On Production And Use Of Silage For A Dairy Enterprise, A J. Corrall, H D. St. C Neal, I M. Wilkinson

IGC Proceedings (1977-2023)

The aim of this work was to examine the influence of variations in grass management and methods of ensiling on the economics of milk production for autumn-calving cows. A simulation model was developed to combine knowledge of plant and animal responses and their interactions in order to calculate the effects of management options on economic balances. Input data were compiled for herbage production from cut­ting strategies that gave herbage at four digestibilities, at two levels of applied nitrogen, and in three climates. Experimental evidence was also collated to construct matrices of loss of dry matter and changes in digestibility and …

Role And Effectiveness Of U.S. Universities In Training Professionals For Work In International Agriculture, D F. Fienup

IGC Proceedings (1977-2023)

Problems of hunger, malnutrition, and world food security became a major world concern in the 1970s. Two U.S. presiden­tial reports have emphasized the seriousness of the situation and the need to expand food production and distribution, par­ticularly in the less-developed countries (LDCs) where hunger is most severe. The LDCs need to strengthen agricultural teaching, research, and extension in order to develop and disseminate the ap­propriate technology farmers need to increase food production. The most limiting factor is often the lack of trained scientists to provide local leadership and expertise. Here U.S. and other developed-country universities are needed to train LDC …

Innovations In Academic Curricula For International Education, D D. Dwyer

IGC Proceedings (1977-2023)

A major contribution of U.S. AID to developing countries has been to support the education of host-country nationals. This form of assistance has not been without its problems, as both AID and the universities themselves have been less than totally ef­fective in meeting the challenge of providing an education that best serves the student and the home country. In most developing countries, the B.S. degree serves as an adequate scientific foundation for staffing agricultural programs. This is not to argue against higher levels of education but to state that faster progress could be made by spreading the educational efforts among …

Effective Training For International Students, G W. Thomas

IGC Proceedings (1977-2023)

The observations made in this paper are based upon contacts with international students, both in the U.S. and in many other countries. Two of the most serious problems in increasing world food production in the developing world are (1) lack of trained people, and (2) technology transfer. A third constraint, inadequate research, is also critical but not of the magnitude of the other two, since there is a great amount of research information available that is not being adequately ut:ilized. Training needs fall into two general categories in which deficiencies are obvious: (1) graduate-level programs, particularly in the applied fields …

West Virginia University Allegheny Highlands Project: A Ten-Year Experiment In Technology Transfer, B S. Baker, P E. Lewis, E K. Inskeep, R H. Maxwell

IGC Proceedings (1977-2023)

West Virginia's livestock industry is characterized by relatively small units located predominantly on rough terrain not suited to highly mechanized or intensive agriculture. As a result of changes in West Virginia agriculture during the last 40 years, there has been a reduction in support from agribusiness, marketing alternatives, and profitability. Some agriculturalists contend that if present technology were adopted, beef and sheep farms in mountainous regions could be profitable and could contribute to the local economy. The objective of the Allegheny Highlands Project (AHP) was to test the effectiveness of a method for delivering technological information to farmers to promote …

Legume Renovation Of Grass-Dominant Swards: A Data-Based Educational Program That Led To Adoption Of Practices By Kentucky Farmers, J K. Evans, G D. Lacefield, W C. Thompson

IGC Proceedings (1977-2023)

Kentucky has about 2.83 million ha of forage crops, most of which are on land with moderate to severe erosion hazards. By the early 1950s, one cultivar (Kentucky 31) of tall fescue (Festuca arundinacea Schreb.) had been seeded on a relatively large area. Legumes grown with grasses had been showri to improve quality of forage markedly. Legumes were lost from many Kentucky grass fields during the relatively dry summers of 1953 and 1954. Research on legume establishment was expanded at the University of Kentucky in 1955. By 1959 sufficient data had been accumulated to formulate recommendations and begin educational programs …