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Solvent-Dependent Cytotoxicity Of Peperomia Pellucida Leaf Extracts Against Mcf-7 Breast Cancer Cells, Ade Arsianti, Rafael Erlangga Bagas Pratama, Steven Gill, Diyah Kristanty

Indonesian Journal of Medical Chemistry and Bioinformatics

Background: Breast cancer remains a major global health burden. Particularly in Indonesia, limitations of conventional chemotherapy, such as severe side effect, toxicity, and high cost, indicating the necessitate the development of alternative therapeutic agents. Peperomia pellucida, a medicinal plant rich in bioactive phytochemicals, has shown promising anticancer potential; however, its cytotoxic activity against MCF-7 breast cancer cells and the influence of solvent polarity remains underexplored. This study aimed to evaluate the cytotoxic effects of P. pellucida leaf extracts which are prepared using different polarity of solvents (ethanol, ethyl acetate, and n-hexane).

Method: Dried leaf powder of P. pellucida was …

Investigating Tumor-Stromal Interactions That Drive Breast Cancer Angiogenesis And Metastasis, Alessandra Dimauro

Arts & Sciences Graduate Student Theses and Dissertations

Metastatic breast cancer remains one of the most difficult cancers to treat, with a five-year survival rate that drops from 99% for localized disease to just 33% once distant metastases are present. This disparity reflects a fundamental gap in our understanding of how breast tumors spread and highlights the urgent need to improve our understanding of breast cancer metastasis to aid in development of novel therapeutics. Emerging evidence establishes that metastasis requires interactions between tumor cells and non-tumor stromal cells within the tumor microenvironment. The first aim of this dissertation investigates the role of Discoidin Domain Receptor 2 (DDR2), a …

Scorpiops Walteri Sp. N. (Scorpiones: Scorpiopidae) From Myanmar, František Kovařík

Euscorpius

A new species Scorpiops walteri sp. n. (Scorpiopidae) is described from Myanmar, fully illustrated with color photos showing its morphology and habitus. Scorpiops walteri sp. n. is the only species of the genus with a combination of the following five characters: chelal trichobothrium Eb₃ located in middle of manus between trichobothria Dt and Est; patella of pedipalp with 11–12 ventral and 19 external trichobothria; fingers of pedipalps strongly undulate in male; pedipalp movable finger with ca. 47 IAD; and chela length to width ratio 2.8 in male.

Petrographic Analysis Of Pottery From A Middle Bronze Age Ii Site West Of Tell Qasille (Pp. 129–131), Anat Cohen-Weinberger

'Atiqot

In this study, 32 vessels were examined from the Middle Bronze Age II settlement, tombs and kilns to determine the nature of the raw materials. Most of the samples were related to the same petrographic group, the nature of which points to a coastal origin. The results of this study suggest a homogenous raw material for the examined assemblage.

Chalcolithic Burial Sites At Ma‘Abarot And Tel Ifshar (Pp. 45–63), Yosef Porath

'Atiqot

The two burial sites are located in the Sharon plain, south and north of Naḥal Alexander. The kidney-shaped cave at Ma‘abarot was hewn in the kurkar sandstone. All the burials in the cave were secondary. Of the 63 burials identified, 42 were placed in three types of ceramic vessels—house-shaped ossuaries, chest-shaped ossuaries and large domestic kraters—and 21, in ‘bone heaps’. The finds consisted of pottery vessels typical of the Chalcolithic period, e.g., V-shaped bowls and fenestrated bowls and bottles, and a flake from a flint digging tool. At Tel Ifshar, two burial places were discovered, both damaged by infrastructure work. …

Mapping And Modeling Threat-Evoked Brain States After Early Life Adversity, Taylor W. Uselman

Biomedical Sciences ETDs

Early life adversity (ELA) increases lifelong neuropsychiatric vulnerability. Yet how ELA reorganizes brain-wide activity and circuit coordination across later experience remains unclear. This dissertation tests the hypothesis that ELA alters adult brain-wide activity and responses to threat through disrupted coordination among neural systems that regulate emotional experience, including prefrontal-limbic and monoaminergic systems. Longitudinal manganese-enhanced MRI of adult mice exposed to standard or fragmented early care, combined with computational processing and statistical modeling, quantified brain states before, during, and long after innate predator threat. These studies established that acute threat evokes large-scale, distributed brain activity that evolves over time. ELA potentiates …

From Chromosomes To Precision Therapy: Clinical Cytogenetics And Cytogenomics In The Era Of Genomic Medicine, Jinglan Liu

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

No abstract provided.

The Evolution Of Regulatory Architectures Underlying Phenotypic Plasticity, Shweta Agarwal

Biology ETDs

Phenotypic plasticity refers to a mechanism where a single genotype results in distinct phenotypes in response to distinct environments, making it a key mechanism for persistence under environmental change. Phenotypic plasticity can arise from two distinct regulatory architectures: epistatically regulated plasticity or environmentally sensitive loci. The evolutionary forces shaping these architectures remain unclear. I use the recombination modifier framework to explore how the regulatory architecture of plasticity evolves across a range of selection regimes, including the rate of environmental change and the costs and benefits of plasticity. I find that the evolution of the regulatory architecture of phenotypic plasticity requires …

Autotrophic And Heterotrophic Contributions To Soil Co2 Fluxes And Variability In A Chihuahuan Desert Grassland, Savannah B. Sanchez

Biology ETDs

Drylands are major drivers of interannual variability in the global terrestrial carbon sink, and their influence is expected to increase as climate change promotes the expansion of hotter and drier regions. Predicting the consequences of these shifts remains challenging because ecosystem models are largely developed for mesic systems, which differ from drylands in water and nutrient availability, decomposition rates, respiration dynamics, and microbial communities. Improving our understanding of carbon cycling in drylands is therefore critical. This research focuses on soil CO₂ efflux, a major pathway of terrestrial carbon release driven by plant root respiration and microbial decomposition. I investigated the …

Behavioral Analysis Of Cryptoprocta Ferox At The Central Florida Zoo And Botanical Gardens, Rita R. Albert

The Pegasus Review: UCF Undergraduate Research Journal

The fossa, Cryptoprocta ferox, is the largest carnivorous mammal endemic to the island of Madagascar. C. ferox is currently listed as a vulnerable species by the IUCN Red List (2015) due to deforestation. Most fossas are now located in zoos for breeding and repopulation purposes, but research on captive mating has been limited due to the low numbers of successful breeding attempts in captivity. The Central Florida Zoo and Botanical Gardens is home to two fossas, one male and one newly introduced female. This study notes behavioral changes in captive mating fossas, with a focus on the male fossa …

Artificial Intelligence-Driven Framework For Enhancing Risk Management In The Architectural Design Process In Egypt, Ayman Ahmed Ezzat Othman, Nadia Mohamed Algheetany, Fatma Othman Alamoudy

Mansoura Engineering Journal

The construction industry is crucial for achieving the United Nations Sustainable Development Goals (SDGs). However, it faces significant risks during the Architectural Design Process (ADP) that affect project quality, cost, schedule, and sustainability. Due to its limitations, traditional risk management (RM) is often inadequate for addressing these challenges. This study aims to develop a novel Artificial Intelligence (AI) driven framework to enhance RM practices in Egyptian Architectural Design Firms (ADFs) through proactive RM and improved data-driven decision making. A mixed methods research approach was adopted to achieve the research objectives. A comprehensive literature review examined RM, ADP, and AI applications …

Toxic Relationships: Characterization Of A Putative Virally Encoded Toxin In The Thermophilic Archaeal Fusellovirus Ssv1, Jonathan C. Abshier, Patrizia L. Alpapara, Guasåli Tomokane, Kenneth M. Stedman

Center for Life in Extreme Environments Publications

Mechanisms for the maintenance of chronic viruses are poorly understood, particularly for archaeal viruses. Here, we identify the product of Sulfolobus spindle-shaped virus 1 (SSV1) ORF a291 as a putative virally encoded toxin required for growth inhibition but dispensable for viral replication and virion production. Viruses lacking ORF a291 replicated their genomes and formed morphologically normal spindle-shaped particles, yet failed to inhibit the growth of uninfected Saccharolobus solfataricus. Substitution of residues at a predicted N-terminal signal peptide cleavage site abolished growth suppression without affecting replication, suggesting that secretion is essential for toxin function. Despite primary sequence divergence among fusellovirus …

Genetic Associations With Pulmonary Embolism Among Those With A Deep Vein Thrombosis: The International Network Against Venous Thrombosis Consortium, Susana Lozano-Esparza, Gabrielle E Shakt, Jennifer A Brody, Angel Martinez-Perez, Matthew P Conomos, Marine Germain, Katharine R Clapham, Maris Teder-Laving, Astrid Van Hylckama Vlieg, Anni Kauko, Florian Thibord, Ohanna C L Bezerra, Girish N Nadkarni, Gaëlle Munsch, Therese Haugdahl Nøst, Ellen L Goode, Yuekai Ji, Daniel I Chasman, Alexander P Reiner, Constance Turman, Kerri L Wiggins, Colleen M Sitlani, Juan Carlos Souto, Pamela L Lutsey, Tiffany Bellomo, Estonian Biobank, Million Veteran Program, Ruifang Li-Gao, Aleksi Kristian Winstén, Ming-Huei Chen, Ron Do, Lénaick Gourhant, Kristian Hveem, Sebastian M Armasu, Noémie Saut, Nathan Pankratz, Franco Giulianini, Jeffrey Haessler, Mingyang Song, Robert Olaso, Eric Boerwinkle, Daniel Dochtermann, Jose Manuel Soria, Stephen S Rich, David Smadja, Satoshi Koyama, Frits R Rosendaal, Teemu Niiranen, France Gagnon, Ha My T Vy, Neil Zakai, Catherine Lemarie, Anne Heidi Skogholt, Jean-François Deleuze, James S Pankow, Paul M Ridker, Yuxi Liu, Kenneth M Rice, Saiju Pyarajan, Mary Cushman, Jospeh Emmerich, Bruce M Psaty, Pradeep Natarajan, Andrew D Johnson, Marc A Rodger, Pierre Suchon, Francis Couturaud, Pierre-Emmanuel Morange, Weihong Tang, Charles Kooperberg, Christopher Kabrhel, Maria Sabater-Lleal, David-Alexandre Trégouët, Alisa S Wolberg, Scott M Damrauer, Nicholas L Smith

Children’s Nutrition Research Center Staff Publications

Background: Venous thromboembolism (VTE) includes deep vein thrombosis (DVT) and pulmonary embolism (PE), the latter of which often originates from DVT and can be fatal.

Objectives: The aim of this study was to identify causal genetic factors that alter risk of PE among those with a presumed DVT.

Methods: Using a case-only design of VTE, we conducted meta-analyses of genome-wide association studies (GWASs) in the International Network Against Venous Thrombosis Consortium. Among participants diagnosed with VTE, each study identified those who had a clinically reported PE, with or without a clinically reported DVT; those remaining had isolated DVT. Logistic regression …

Effects Of Variable Light On Sexual Competition In Kelp Species, Jaycee Lanza

Master's Theses

As foundation species, kelps significantly influence the structure and composition of their surrounding habitat by modifying environmental conditions, including light levels, currents, nutrient availability, sedimentation, and seawater chemistry. Light, in particular, is a critical resource that varies greatly within kelp forests, affecting kelp reproductive processes such as gametogenesis, fertilization, and recruitment, which are vital to population persistence. Biological factors, such as density-dependent competition for resources and sexual competition with other kelps, can also affect such processes. To understand how environmental variables influence kelp populations, it is crucial to recognize their combined effects. This study investigated the impacts of light levels …

Using Fecal Diagnostic Tests To Assess The Prevalence Of Fasciola Hepatica In The United States Sheep Population, Brooke A. Delcambre

LSU Master's Theses

Fasciola hepatica, the common liver fluke, is endemic in the Pacific Northwest and Gulf Coast of the United States. To provide updated information on F. hepatica prevalence, as part of the USDA National Animal Health Monitoring System Sheep 2024 study, sheep operations in the top 30 sheep-producing states were identified, and producers were invited to participate. For each operation, feces were collected from at least eight sheep and pooled to form a single composite sample. Two methods were used to identify sheep infected with F. hepatica: fecal sedimentation and indirect sandwich ELISA. At the national level, across 318 operations, 7.1 …

In Silico Analysis Of Vaginal Microbiota In Female Reproductive Health Using Ncbi Database And Blast, Arleni Bustami, Ariyani Kiranasari, Engla Merizka

Indonesian Journal of Medical Chemistry and Bioinformatics

Background: The vaginal microbiota plays a critical role in maintaining female reproductive health. Beneficial bacteria, particularly Lactobacillus species, contribute to vaginal homeostasis, whereas dysbiosis-associated bacteria such as Gardnerella vaginalis and Prevotella bivia have been linked to bacterial vaginosis and reproductive tract disorders. Publicly available nucleotide databases and bioinformatic tools provide accessible resources for studying microbial species associated with vaginal health. Objective: This study aimed to perform a simple in silico analysis of representative vaginal microbiota using publicly available 16S rRNA gene sequences retrieved from the NCBI database and analyzed using BLAST and phylogenetic approaches. Methods: Representative 16S rRNA gene sequences …

Analysis Of Inorganic Element Content And Health Risk Assessment Of Ranunculi Ternati Radix From Different Origins, Li Juan, Geng Xiaotong, Yao Weijie, Zhou Tao, Bi Jingjing

Food and Machinery

[Objective] The variations in inorganic element content and the safety of heavy metals and harmful elements in Ranunculi ternati radix from different origins were investigated. [Methods] The quantitative analysis of 24 elements in the samples was performed by inductively coupled plasma mass spectrometry (ICP-MS). Health risk assessment was conducted using estimated daily intake (EDI), target hazard quotient (THQ), and carcinogenic risk (CR). Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used for origin identification analysis and differential element screening. [Results] All 25 Ranunculi ternati radix samples from different origins contain abundant macronutrients (K, Na, Ca, and Mg) …

Effects Of Various Extraction Methods On The Performance Of Corn Fiber Gum-Pea Protein Composite Gels, Wu Chenglong, Jiang Zhili, Huang Lilin, Tang Leiming, Sun Xiaohong

Food and Machinery

[Objective] This study aims to investigate the effects of three extraction methods - alkaline extraction, enzymatic extraction, and acid extraction - on the properties of corn fiber gum (CFG)-pea protein composite gels. [Methods] CFG was extracted from corn bran using the three methods. The gel strength, water-holding capacity, rheological properties, and microstructure of the CFG-pea protein composite gels are evaluated. [Results] The CFG extracted by enzymatic extraction increases the gel strength of pea protein gels from (27.20±2.45) g to (65.80±2.67) g, and the water-holding capacity from (40.60±3.40)% to (75.80±5.10)%. Additionally, it forms a more compact and ordered network structure with …

Effects Of Extraction Methods On The Composition And Physicochemical Properties Of Flavonoids From Lycium Barbarum Leaves, Li Juanxia, Li Jianxin, Gao Jianying, Mai Wenting, Fan Yanli, Ma Jinchao, Meng Xuemei

Food and Machinery

[Objective] To investigate the effects of different extraction methods on the efficacy, chemical composition, and functional group characteristics of flavonoids in Lycium barbarum leaves. [Methods] This study comparatively analyzes the effects of three methods - ultrasound-assisted ethanol extraction (UAE), microwave-assisted ethanol extraction (MAE), and aqueous two-phase system (ATPS) - on the yield, composition, and in vitro antioxidant activity of flavonoids from L. barbarum leaves. [Results] UAE and MAE achieve the highest yield of flavonoids from dried L. barbarum leaves. The main components are rutin, quercetin, chlorogenic acid, and kaempferol. The antioxidant capacity of the flavonoid extract from dried L. barbarum …

Heat Transfer Analysis And Packaging Optimization Design Of Self-Heating Rice, Sun Haowen, Li Huan, Wu Xiaomeng, Mao Like

Food and Machinery

[Objective] To further understand the influence of the heat transfer process on the quality of self-heating foods and improve the heating rate of existing self-heating rice products. [Methods] A combination of virtual simulation and experimental methods was used to analyze the actual temperature variations during the heating process of self-heating rice and the effects of heat transfer on rice texture. A three-dimensional heat transfer model of self-heating rice was established using COMSOL software to investigate the heat transfer process, and the packaging design of self-heating rice was optimized with the objective of maximizing the heating rate. [Results] After reheating and …

Dynamic Evolution Law Of Volatile Flavor Components During The Aging Process Of Sauce-Flavored Baijiu, Dai Jiahui, Wang Yanan, Kang Xiaoning, Dai Wenting, Wang Shiping

Food and Machinery

[Objective] To investigate the aroma changes of sauce-flavored Baijiu during different aging periods, and to provide a theoretical basis for Baijiu aroma analysis and modern processing technology research. [Methods] Electronic nose, gas chromatography -mass spectrometry (GC-MS), and gas chromatography-ion mobility spectrometry (GC-IMS) were employed. Sauce-flavored Baijiu with different aging times (original liquor, 1-year aged, and 5-year aged) was used as the research objects. Combined with multivariate statistical methods, including principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS -DA), the differences in volatile flavor compounds of Baijiu with different aging times were analyzed. [Results] A total of 1 …

Effect Of Cultivation Methods On Volatile Components In Morchella Esculenta, Liu Ni, Jiang Bo, Li Lang

Food and Machinery

[Objective] To comprehensively reveal the flavor and quality characteristics, and differences of Morchella esculenta under different cultivation methods, and systematically evaluate their volatile aroma components. [Methods] Qualitative and quantitative analyses are conducted on volatile aroma components in M. esculenta cultivated in greenhouses, under forests, and in caves in the vicinity of FAST by headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) techniques. The contribution of different components to the overall flavor is assessed by the relative odor activity value (ROAV) method. Based on this, principal component analysis and cluster analysis are conducted, achieving a comprehensive evaluation of the flavor …

Microwave-Assisted Enzymatic Hydrolysis Of Tibetan Sheep Blood Protein And Analysis Of Product Characteristics, Yu Xilu, Gu Xuedong, Chen Yujin, Zhang Hongxin, Ciren Danzhu

Food and Machinery

[Objective] This study aims to utilize fresh Tibetan sheep blood as raw material, investigate the enhancing effect of microwave-assisted technology on enzymatic hydrolysis efficiency, and achieve high-value utilization of Tibetan sheep blood. [Methods] A microwave-assisted trypsin hydrolysis method is employed to enzymatically hydrolyze the proteins. The optimal hydrolysis conditions are established through single-factor experiments and response surface methodology. The resulting hydrolysates are characterized using scanning electron microscopy (SEM) and a fully automated amino acid analyzer. [Results] The optimal hydrolysis conditions are determined as follows: microwave temperature of 40 ℃, microwave time of 50 min, enzymatic hydrolysis time of 240 min, …

Rapid Detection Of Viable Salmonella Based On Specific Phage Infection Combined With Fluorescent Rpa, Liu Daofeng, Zhai Yujie, Tan Yucheng, Shan Shan, Lu Jun, Zhong Ziqing

Food and Machinery

[Objective] To establish a rapid detection method for viable Salmonella based on specific phage infection and recombinase polymerase amplification (RPA) in response to the key challenge that traditional molecular biological detection methods have difficulty in conveniently distinguishing between viable and non-viable foodborne pathogens. [Methods] Taking advantage of the characteristic that O-1 phage only proliferates in viable bacteria, the phage is added to the enriched sample. After further incubation, total DNA is extracted. Fluorescent RPA is used to determine whether the phage has significantly proliferated, so as to judge the presence of viable Salmonella in the original sample. [Results] After optimization, …

Establishment Of An Indirect Competitive Elisa For Sulfadimethoxine, Han Lu, Huang Mingyue, Yu Xiang, Li Sijia, Cao Jinbo, Wang Yao, Kang Huaibin

Food and Machinery

[Objective] To establish an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of sulfadimethoxine (SDM) in animal-derived foods. [Methods] An artificial antigen is synthesized by conjugating SDM with carrier proteins by the diazotization method. A highly sensitive antibody against SDM is prepared by immunizing animals. Based on this, an ic-ELISA is established and the detection system is optimized. Its detection performance is characterized, and spiked recovery tests are carried out. [Results] When the concentration of the coating antigen is 0.500 μg/mL, the dilution ratio of the horseradish peroxidase-labeled goat anti-rabbit secondary antibody is 1:5000, the reaction time of the …

Authenticity Identification Of Camellia Seed Oil Based On Gas Chromatography And Chemometrics, Li Jingyan, Li Qin, Han Bo, Zhang Ruixue

Food and Machinery

[Objective] To establish a method for authenticity identification of camellia seed oil. [Methods] The fatty acid composition of camellia seed oil and six common vegetable oils are determined by gas chromatography. Principal component analysis (PCA) is employed to explore the distribution patterns of fatty acids among the vegetable oils and to screen for potentially adulterated oils. Partial least squares discriminant analysis (PLS-DA) is applied to establish a model between fatty acid profiles and actual adulterated oils. Finally, multiple stepwise linear regression analysis is used to establish quantitative analysis models for adulteration in camellia seed oil. [Results] Among the six common …

Migration Volume Determination And Migration Patterns Of 11 Antioxidants From Degradable Polylactic Acid Straws, Luo Minting, Wu Zhihang, Hu Tengxin, Xu Qingpeng

Food and Machinery

[Objective] To establish a method for authenticity identification of camellia seed oil. [Methods] The fatty acid composition of camellia seed oil and six common vegetable oils are determined by gas chromatography. Principal component analysis (PCA) is employed to explore the distribution patterns of fatty acids among the vegetable oils and to screen for potentially adulterated oils. Partial least squares discriminant analysis (PLS-DA) is applied to establish a model between fatty acid profiles and actual adulterated oils. Finally, multiple stepwise linear regression analysis is used to establish quantitative analysis models for adulteration in camellia seed oil. [Results] Among the six common …

Determination Of Five Fluorescent Whitening Agents In Prepared Dishes By Gel Permeation Chromatography-High Performance Liquid Chromatography, Li Bingbing, Wang Chunlei, Huang Zhenzhen, Dai Li, Wang Kun

Food and Machinery

[Objective] To establish a method for the determination of five fluorescent whitening agents (FWAs) in prepared dishes using gel permeation chromatography combined with high-performance liquid chromatography (GPC-HPLC). [Methods] The prepared dish samples were homogenized, ultrasonically extracted with acetonitrile, purified and reconstituted by GPC, then filtered before being analyzed by HPLC. The separation was performed on a Waters Symnetry Shield PR-C18 column (4.6 mm ×250 mm, 5.0 µm) using acetonitrile and water as the mobile phase with gradient elution. [Results] The five FWAs in the prepared dishes showed good linearity within the range of 2~500 µg/L, with correlation coefficients greater than …

Determination Of Migration Level Of 4-Methyl- 1-Pentene And 1-Decene From Food Contact Materials Using Headspace-Gas Chromatography, Liu Afang, Chen Ranbing, Li Zheng, Kang Feng, Zhao Jinyao

Food and Machinery

[Objective] This study aims to establish an analytical method for detecting the migration levels of 4-methyl-1-pentene and 1-decene monomers from food contact materials into four food simulants, including 4% acetic acid, 10% ethanol, 20% ethanol, and 50% ethanol. [Methods] Chromatographic columns of different polarities and specifications are compared to select the optimal column. The headspace equilibrium temperature and equilibrium time are optimized using peak area as the evaluation index. Methodological validation is performed by conducting recovery experiments at low, medium, and high spiking levels using the soaking solutions of the negative sample of the four food simulants as the matrix, …

A Surface Defect Detection Model For Fused Images Of Fruits Based On Improved Resnet 18, Si Haiping, Zhao Wenrui, Zhao Yuyang, Li Tingting, Fernado Bacao, Li Yanling

Food and Machinery

[Objective] To enhance the accuracy of detecting fruit surface defects caused by minor mechanical friction during transportation. [Methods] A surface defect detection model for fused images of fruits based on improved ResNet 18 is proposed. To address the challenge of multi-scale feature extraction, the model incorporates a MELA module designed to capture image details more comprehensively. Additionally, an energy function-based regularization loss function is introduced to collaboratively optimize the training process alongside the cross-entropy loss function. Furthermore, a transfer learning strategy is employed to mitigate overfitting. [Results] Experimental results demonstrated that the improved model exhibits exceptional performance in fruit surface …