Digital Commons Network^™

Analysis On Thermo-Viscous Steady Fluid Motion Over An Impermeable Infinitely Stretched Horizontal Moving Rectangular Surface, P. Krishna, N. Pothanna, K. Spandana

Mansoura Engineering Journal

This study presents analysis on thermo-viscous steady fluid motion over an impermeable infinitely stretched horizontal moving rectangular surface. The numerical results have been found employing R-K method of order 6 shooting techniques developed in Mathematica software ND solve for the flow adaptable equations comprising temperature and velocity. The flow behavior and the impacts of material constraints on the flow region have been analyzed and deliberated taking the help from the generated graphs. The variations of these flow fields have been studied for wide spectrum of physical characteristics which influences the nature of thermo-viscous fluid. The impact of viscosity, constant pressure …

Enhancer Rna Transcription Near Segmentation Gene Enhancers Can Be Analyzed In Situ Using Fish, Christine Mau, Benjamin Schmid, Ezzat El-Sherif

School of Integrative Biological & Chemical Sciences Faculty Publications

Enhancer RNAs (eRNAs) are non-coding transcripts produced at enhancer regions, which appear to be involved in transcriptional regulation. Up to date, these have been primarily investigated using labor- and cost-intensive genomic techniques. However, the precise mechanisms by which eRNA transcription or eRNA transcripts themselves mediate transcriptional regulation remain unclear. Here, we present a novel experimental approach that allows us to analyze characteristics of eRNA transcription in fixed and live whole Drosophila melanogaster embryos. We employ the anterior–posterior patterning genes as a model system to investigate the dynamics of eRNA expression, utilizing an imaging-based approach. We combined high-sensitivity fluorescence in situ …

Contribution Of Animals To Biomedical Advancements In The Current Era: A Narrative Review, Leena Rajathy Port Louis, Mani Rajarathinam, Prithiviraj Nagarajan, Divya Sekar, Nisha Perumal

The Thai Journal of Pharmaceutical Sciences

Animal research has played a major role in the development of vaccinations and therapies for illnesses such as tetanus, polio, meningitis, hepatitis, and Severe Acute Respiratory Syndrome Coronavirus 2. For every prescription drug available today, animal testing and research have contributed immensely. This narrative review aims to explore the contribution of animals to biomedical advancements in the current era, which involved a search of peer-reviewed literature using electronic databases such as Scopus, Web of Science, and PubMed. In current biomedical research, animals help develop new treatments for long-term illnesses such as cancer, HIV, and Alzheimer’s. Animals also aid in the …

Adaptative And Structured Optimization Stockwell Transform For Robust Power-Quality Disturbances Detection, Moudjibatou Afoda, Séna Apeke, Filippo Gatti, Alessio Iovine, Léonce W. Tokam, Guy M. Toche Tchio, Ouro-Djobo S. Sanoussi

Mansoura Engineering Journal

The monitoring of electrical power quality requires advanced analysis techniques capable of accurately detecting and localizing non-stationary disturbances. This work proposes a structured and adaptive optimization of the Stockwell Transform (ST), referred to as Two-Power Optimization (TPO). A multiparametric Gaussian window governed by four parameters (m, p, k, r), is introduced, and a genetic algorithm is applied independently to each signal to optimize these parameters. The optimization process adapts the analysis scale to the local characteristics of the signal while preserving the physical consistency of the window function. Simulation results on synthetic power quality signals demonstrate that the proposed method …

Finite Element Modelling On A Non-Linear Radiative Hybrid Nanofluid Flowing Through A Rotating Disk, N. Janaki Phani Madhuri, Md. Shamshuddin

Mansoura Engineering Journal

This study develops a finite element model to investigate the MHD flow and heat transfer phenomena of Cu–H₂O and Cu–Fe₃O₄–H₂O hybrid nanofluids flow across a spinning disk modelling by the Tiwari–Das model. The governing non-dimensional momentum and energy equations account for the effects of buoyancy, nonlinear radiation, and ohmic heating. The nonlinear coupled system is solved with the element method (FEM) to find the velocity and temperature fields. Parametric studies will investigate the effects of the magnetic field, buoyancy, volume fraction of nanoparticles, and radiation. Skin friction and Nusselt number are also reported. The results show that with the hybrid …

Real-Time Iot-Enabled Dissolved Oxygen Monitoring And Automated Wastewater Recirculation In Constructed Wetlands, Sivasankar Pandiarajan, Vanitha Sankararajan, Devarinti Chandu

Mansoura Engineering Journal

Wastewater treatment is necessary for environmental conservation and combating water pollution. This study demonstrates real-time dissolved oxygen (DO) monitoring in constructed wetland treatment. The system would ensure an adequate supply of oxygen through automated water circulation whenever the level is below 4 mg/L, and shut down when it reaches the normal level. From experiments conducted, it was observed that within three automated circulation processes, it is possible to increase DO concentrations from 0.5 mg/L to 5.0 mg/L. Additionally, Total Dissolved Solids reduced from 1,380 ppm to 920 ppm. pH also stabilized from 8.38 to 7.9. In this system, data is …

Bridging Machine Learning And Climate Futures: A Framework For Explainable, Self-Directed (Agentic) Ai Models In Monitoring, Mitigation, And Governance Of Anthropogenic Climate Impacts, Vijayanandh Rajamanickam, Ketaki Kulkarni, Vishwanadham Mandala, Ozgur Kisi

Mansoura Engineering Journal

Environmental and societal damage due to anthropogenic climate change demands detection and mitigation strategies that are both effective and accountable. Machine Learning (ML) appears to offer a powerful set of tools but remains unexploited in these areas. A key barrier remains its poor ability to draw causal inferences about external systems, an essential requirement for meaningful continued monitoring, acting on, and shaping of the climate. Explainable, self-directed learned models operate by attributing environmental changes and deciding how best to model the resulting dynamics—they offer a natural solution. Recent developments in computational ecology, meteorology, and ML are combined to propose a …

Ameliorating Chromium-Induced Stress In Maize Through Phosphorus And Melatonin Application, Zunaira Zafar, Shoaib Ahmad, Muhammad Akhtar, Shafaqat Ali, Sarah Owdah Alomrani, Muhammad Ali Alshehri

Turkish Journal of Agriculture and Forestry

Chromium (Cr+6)  toxicity is a significant environmental stress factor that severely hampers plant growth and productivity. This study aimed to evaluate whether the combined application of phosphorus fertilizer and melatonin could alleviate chromium induced stress in maize  (Zea mays L.).  A foliar spray of melatonin (50 µM) was applied alongside two phosphorus sources—phosphoric acid (PA) and diammonium phosphate (DAP), each at 60% of the recommended rate. Two maize cultivars (FH-1046 and YH-1898) were grown under chromium stress (0, 15, and 30 mg kg–1) to assess treatment effects. The results demonstrated that FH-1046 plants treated with PA + melatonin under noncontaminated …

Derived Smoke Solution Mitigates Drought Stress In Forage Pea By Enhancing Antioxidant Defense Mechanisms, Medi̇ne Çopur Doğrusöz

Turkish Journal of Agriculture and Forestry

This study was conducted to enhance plant resilience against drought stress, a major threat to agricultural production, by utilizing an eco-friendly application derived from agricultural waste. We investigated the protective effect of a smoke solution, derived from poppy harvest residues, on the biometric and biochemical parameters of two forage pea (Pisum sativum spp. arvense L.) varieties (GAP Pembesi and Özkaynak). Seeds were primed with 1% and 10% smoke solutions and grown under three distinct conditions: normal irrigation (control), moderate drought, and severe drought. Parameters including biometric traits, chlorophyll pigments, secondary metabolites, and antioxidant enzyme activities were analyzed. The smoke solution …

Prediction Of Furniture Exports In Türkiye Using Machine Learning Methods, İlker Akyüz, Nadi̇r Ersen, Ti̇muçi̇n Bardak, Selahatti̇n Bardak

Turkish Journal of Agriculture and Forestry

In this study, it was aimed to predict Türkiye’s furniture exports for the period from January 2010 to May 2025 using machine learning methods based on macroeconomic indicators. The dataset consisted of 10 economic variables, including the furniture industry production index, capacity utilization rate, import volume, total exports, real effective exchange rate, producer price index, money supply, and oil prices, with 185 observations per variable and a total of 2035 data points. During the data preprocessing phase, no missing or outlier values were detected, and the data were divided into 70% training and 30% testing subsets. Decision tree, random forest, …

Ozonated Water Delays The Microbial Growth And Sensory Quality Loss Of Capia Pepper Fruit During Cold Storage, Yasi̇n Şi̇mşek, Mehmet Ali̇ Koyuncu, Derya Erbaş

Turkish Journal of Agriculture and Forestry

The aim of the present research was to evaluate, for the first time, the effect of ozonated water treatments on the postharvest quality of capia pepper (Capsicum annuum L. ‘Kaptan’). The peppers were harvested at the optimum time and transported to the laboratory immediately. Uniform and quality fruit was selected and precooled. After precooling, the fruit was divided into four groups. The first three groups were immersed in ozonated water at three different concentrations (1.0, 2.0 and 3.0 ppm) for 15 min. The last group (control) was immersed in distilled water for 15 min. The peppers were kept at room …

Jnk Signaling Impacts Car-T Cell Effector Function, Charles Kuhlmann

ETDs from 2020-2029

The potent cytotoxic activity of T cells serves as a natural defense against cancers. Chimeric antigen receptor (CAR) T cell therapy represents a strategy for harnessing T cells against advanced tumors. CAR-T cell therapy has seen striking success against hematologic malignancies, prompting its application to new diseases. Ovarian cancer is a highly lethal disease characterized by frequent recurrence and aggressive nature, prompting the investigation of CAR-T cell therapy as a salvage regimen. However, like in other solid tumors, the early performance of CAR-T cell therapy in ovarian cancer is poor. While many strategies exist for improving the longevity of CAR-T …

A Pilot Study Of The Acute Effects Of Isometric Exercise And Aerobic Exercise For Improving Blood Pressure And Vascular Hemodynamics In Hypertensive Adults, Ayih Cedric Edoh

ETDs from 2020-2029

Hypertension (HTN) is one of the most prevalent and modifiable risk factors for developing cardiovascular disease (CVD). Aerobic exercise is a well-known method to lower blood pressure (BP), but recently, isometric exercise has been purported to lower BP to a similar or greater extent. There is little known about the effects of isometric exercise on acute BP and 24-hour ambulatory blood pressure (ABP). Therefore, the purpose of this pilot study was to compare the effects of isometric and aerobic exercise on improving vascular hemodynamics and 24-hour ABP in hypertensive adults. METHODS: Seven participants (age 25-65 years) with a diagnosis of …

Retinoic Acid-Loaded Nanoparticles Enhance Macrophage-Mediated Control Of Leishmania Infantum, Zeynep İşlek, Merve Gündoğdu, Zeynep Di̇la Yüksel

Turkish Journal of Biology

Background/aim: Visceral leishmaniasis, primarily caused by Leishmania infantum, remains a major global health challenge due to limitations in current chemotherapeutic options, including toxicity and emerging drug resistance. Host-directed therapeutic approaches are increasingly recognized as promising alternatives. All-trans retinoic acid (ATRA) is an immunomodulatory molecule with host-dependent effects on macrophage function; however, its therapeutic use is hindered by instability and poor solubility. Solid lipid nanoparticles (SLNs) offer a controlled and biocompatible delivery platform capable of enhancing intracellular drug accumulation.

Materials and methods: ATRA-loaded SLNs were prepared and characterized for size, polydispersity index, zeta potential, and morphology. Their antileishmanial activity was evaluated …

Characterization Of Poly-Β-Hydroxybutyrate And Its Biosynthesis In Priestia Endophytica Ucm B-5715, Alina Kharchuk, Maksym Kharchuk, Maksym Kharkhota, Sergiy Rogalsky, Oksana Tarasyuk, Oleksandr Kisten, Anastasiia Hubina, Illya Kapats, Liliia Avdieieva

Turkish Journal of Biology

Background/aim: Polyhydroxyalkanoates (PHAs) are biodegradable biopolymers with promising applications in biotechnology and medicine; however, the diversity of their biosynthesis within the genus Priestia remains poorly characterized. Although Priestia species have been recognized as potential PHA producers, the detailed genomic architecture and physicochemical properties of the polymers synthesized by these bacteria, particularly in the type strain, remain poorly understood. This study aimed to characterize the poly-β-hydroxybutyrate (PHB) produced by Priestia endophytica UCM B-5715 and to investigate the PHB biosynthetic genes across related strains.

Materials and methods: PHB granules were visualized and measured using fluorescence microscopy, transmission electron microscopy, and scanning electron …

Nutrient Uptake Of Two Microalgae From Culture Medium Under Nickel Treatment, Di̇lara Erdaği, Büşranur Şeker, Celal Caner, Gi̇zem Çeti̇n, Hüseyi̇n Altundağ, Hati̇ce Tunca

Turkish Journal of Biology

Background/aim: Phycoremediation is essential for purifying wastewater of heavy metals to ensure a sustainable environment, as these components can be detrimental to organisms upon entering the food chain. Among heavy metals, nickel is vital at low concentrations due to its presence in the active sites of many metalloenzymes in algae. However, at high concentrations, it disrupts the functioning of many metabolic reactions. This study examines the effects of nickel treatment on the uptake of macro- and micronutrients in medium by algae via ion exchange pathways or in competition with other metals. The study aims to determine the effects of nickel …

Plant Extract-Enriched Mineral Beverages: Effects Of Temperature And Ultrasound Processing On Bioactive Content, Eli̇f Feyza Topdaş, İsa Arslan Karakütük, Memnune Şengül

Turkish Journal of Agriculture and Forestry

Functional beverages derived from herbal extracts have potential health benefits. There is a growing interest in these beverages because they are a sustainable and health-promoting alternative to conventional flavored drinks. This study aimed to produce phenolic rich mineralized herbal beverages using lyophilized purple basil, lavender, and thyme extracts at two different concentrations (2% and 4%). Changes in the physicochemical properties (pH, electrical conductivity, color, and viscosity), total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-Azino-bis(3-ethylbenzothiazoline 6-sulfonic acid (ABTS) radical scavenging assays), and individual phenolic compounds were determined for beverages stored at 4, 25, and …

Ecological Risk Assessment Of Heavy Metal-Microplastic Interactions In Agricultural Soils Irrigated With Secondary Treated Wastewater, Senar Aydin, Osman Mücevher, Arzu Ulvi̇, Fatma Bedük, Özen Merken, Ci̇han Uzun, Mehmet Emi̇n Aydin

Turkish Journal of Agriculture and Forestry

The increased reuse of treated wastewater (TWW) for irrigation in arid and semiarid regions raises environmental concerns regarding microplastics (MPs), specifically their capacity to adsorb and transport heavy metals. This study assesses the quantity of MPs and associated metals (Cd, Cr, Cu, Pb, Ni, and Co) in agricultural soils irrigated with TWW in the Konya region of Türkiye. To this end, a total of 202 soil samples were collected from TWW-irrigated (180) and nonirrigated control fields (22) at two depths (0–10 and 10–20 cm). MPs were extracted from the samples using a density separation technique, identified microscopically, and analyzed for …

Influence Of Crop Diversification On Climate Change Mitigation And Business Profitability: A Study Of The Southern Marmara Region, Türkiye, Engi̇n Tolga Önder, Nuray Demi̇r

Turkish Journal of Agriculture and Forestry

Crop diversification strategies are being put forward to mitigate the negative effects of climate change associated with greenhouse gas emissions, and thus to ensure sustainable food production that is resilient to climate change and to reduce the risks faced by producers. By diversifying their agricultural products producers can insure themselves against the risks associated with climate variations, disease, and pests, contributing to both continuous food production and income flow. The present study investigates the effect of climate change on enterprise income in the Yenişehir district of Bursa province – a region noted for its intensive production of vegetables and other …

“He’S Got The Whole World In His Hands”: Seed Sovereignty And Agricultural Decision Making In Southeastern Kenya, Caroline Stahley

USF Tampa Graduate Theses and Dissertations

Increasing corporate control of the global seed supply and restrictions to local exchange threaten the autonomy of smallholder farmers and Indigenous communities. This project contributes to agricultural sovereignty movements by examining the decisions farmers make to address climate uncertainty, market volatility, and development agendas. Specifically, how is seed saving practiced and understood in relation to sovereignty? It reimagines what global food systems might look like if investment were directed to producers rather than intermediaries, arguing that cultivators lack support, not knowledge or capacity. Across these domains, seeds act as a motif to describe the concerns, innovations, and visions of farmers.

Amending Planting Media With Typha-Derived Biochar For Sustainable Horticulture, Elissa Touma

USF Tampa Graduate Theses and Dissertations

Constructed wetlands are widely used to improve water quality by removing excess nutrients from wastewater and stormwater. Nutrients are stored within wetland vegetation, particularly in fast-growing plants such as Typha. Harvesting vegetation provides an opportunity to permanently remove these nutrients while generating biomass suitable for beneficial reuse. Converting harvested biomass into value-added products such as compost and biochar can support nutrient recovery and sustainable biomass management. In addition, increasing concerns regarding the environmental impacts of peat extraction have created interest in renewable alternatives for horticultural growing media. Therefore, the objective of this study was to evaluate the potential of harvested …

Processing Mechanism And Efficacy Maintenance Strategy Of Ginseng Active Ingredients Based On Spectroscopy Technology, Yang Hantong, Kong Linghui, Jiang Mengmeng, Wang Yingxiu, Li Xiangguo

Food and Machinery

Ginseng, as a medicinal and edible plant, has ginsenosides as its core active components, which are widely applied due to their physiological functions such as antioxidant and anti-inflammatory activities; however, their structures and activities are easily affected by food processing. During processing steps such as cleaning, steaming/boiling, and fermentation, temperature, pH, and microbial enzyme systems alter the types, contents, and stability of active components through reactions such as hydrolysis and oxidation. The intrinsic linkage mechanism of "component transformation-activity-bioavailability" constitutes a core focus and challenge in current research. This study, based on spectroscopic techniques including high-performance liquid chromatography (HPLC), nuclear magnetic …

Design And Test Analysis Of Automatic Disemboweling Device For Chilled Fresh Duck Carcasses, Wang Yuhao, Bai Zongchun, Zhao Shida, Liu Yunping, Zheng Hao

Food and Machinery

［Objective］ To solve the problems of low automation level and high labor intensity in the disemboweling process of the current meat duck slaughtering and processing technology.［Methods］ With chilled fresh duck carcasses as the research object, an automatic disemboweling device for chilled fresh duck carcasses was created by simulating the existing manual disemboweling method. A special disemboweling tool was designed. Mathematical analysis was used to conduct motion analysis and spatial curve fitting of the disemboweling action of the tool, so as to obtain the characteristic equation and analyze the rationality of the design of the duck carcass disemboweling tool. In addition, …

Effects Of Gardenia Extract On Body Weight And Intestinal Mucosal Microbiota In Obese Mice, Liu Qin, Deng Na, Zhou Mengsi, Tan Zhoujin

Food and Machinery

［Objective］ To explore the effects of gardenia extract on intestinal mucosal microbiota and body weight in mice fed a high -fat diet, and to explore the microecological mechanism of gardenia extract in obese mice.［Methods］ The potential mechanism of gardenia extract in treating obesity is predicted by network pharmacology and molecular docking technology. Additionally, 24 SPF C 57BL/6 male mice are randomly assigned into a blank group (8 mice ) and a model group (16 mice ). The model group receives a high-fat diet for 12 weeks, while the blank group receives an ordinary diet. After modeling, 12 successfully modeled mice …

The Effect Of Project-Based Learning Model On Entrepreneurship (Pjbl-E) With A Deep Learning Approach On Students' Problem-Solving And Cognitive Skills, Ricce Oktasari, Irwandi Irwandi, Siti Darwa Suryani

Jurnal Pendidikan Sains

This study aims to examine the effect of the Entrepreneurship-based Project Based Learning (PjBL-E) model integrated with a pedagogical Deep Learning approach on students’ problem-solving skills and cognitive learning outcomes in biology learning. The novelty of this study lies in the integration of entrepreneurship values and pedagogical deep learning principles into Project Based Learning to create contextual and meaningful learning experiences. Entrepreneurship integration encourages students to develop innovative products, identify real-world problems, and apply biological concepts in practical and socio-economic contexts. Meanwhile, the pedagogical Deep Learning approach promotes reflective thinking, conceptual understanding, critical analysis, and the ability to connect theory …

The Effect Of Discovery Learning Model On Students’ Critical Thinking Skills In Biology Learning At Sman 1 Dampelas, Umiyatul Rahma, Mursito S. Bialangi, Amalia Buntu, Fatma Dhafir, Raya Agni, Gamar B. N Shamdas

Jurnal Pendidikan Sains

Critical thinking skills are essential in biology learning; however, many classroom practices remain teacher-centered, limiting students’ opportunities to engage in higher-order thinking processes. Although previous studies have reported the potential of Discovery Learning to enhance critical thinking, evidence regarding its effectiveness in secondary biology education remains limited, particularly in the context of Indonesian high schools. Therefore, this study aimed to examine the effect of the Discovery Learning model on students’ critical thinking skills in biology learning at SMAN 1 Dampelas. A quasi-experimental study employing a nonequivalent control group design was conducted with 48 Grade XI science students, consisting of 24 …

Assessment Of Artificial Oyster Reef Health Based On Water Quality, Oyster Recruitment, And Perkinsosis Infection In Tampa Bay, Christian J. Goldman

USF Tampa Graduate Theses and Dissertations

Providing nursey habitat, regulating nutrients, stabilizing shorelines, increasing biological diversity, improving water quality, and creating three-dimensional environments are some of the ecosystem services of oyster reefs. Oyster reefs are threatened by overharvesting, changes in water quality, pollution, and disease. The Eastern oyster, Crassostrea virginica, is the primary oyster species along Florida’s Gulf coast. This study aimed to evaluate the relationship between multiple parameters on artificial reefs and how they influence the health of these reefs. Here, young (less than five years old) artificial oyster reefs were monitored for one year across three sites in Tampa Bay, FL: Lassing Park (LAS), …

Effects Of Yeast Extract And Reheating Methods On Gel Properties And Warmed-Over Flavor Formation Of Hairtail Surimi, Huang Qianyi, Li Xianghong, Li Shuang, Ma Xiayin, Liu Dongmin, Wang Faxiang, Liu Yongle, Wu Jinhong

Food and Machinery

［Objective］ To control the gel property and warmed -over flavor formation of hairtail surimi products.［Methods］ Surimi gel is prepared using hairtail as the raw material. Then, by examining changes in sensory evaluation, microstructure, gel property,moisture content, and volatile flavor substances, this study investigates the effects of yeast extract addition and reheating methods on gel property and warmed-over flavor formation in hairtail surimi products.［Results］ Yeast extract addition significantly promotes internal structural cross-linking and reduces the number of pores in the product, thereby enhancing the gel properties, including gel strength, hardness, and elasticity. Specifically, an optimal gel property is observed at a …

Preparation , Characterization , And Stability Of Pickering Emulsions Stabilized By Theaflavins-Bovine Serum Albumin Complexes, Fu Zhixuan, Cao Qian, Wu Yuanjie, Wu Haoren, Shi Meng

Food and Machinery

［Objective］ To construct theaflavins (TFs)-bovine serum albumin (BSA ) complex-based Pickering emulsions to enhance the multiple stability of Pickering emulsions.［Methods］ TFs at different mass concentrations (0~20 mg/mL ) were used to prepare complexes with BSA, which were then employed to prepare Pickering emulsions with rapeseed oil. The microstructure, particle size, Zeta potential, and rheological properties of the emulsions were characterized. The effects of TFs mass concentration on the stability of the emulsions under centrifugation, storage, and sodium ion stress were further analyzed.［Results］ TFs formed stable complexes with BSA, and the resulting Pickering emulsions exhibited spherical droplets. As the TFs mass …

Preparation And Fermentation Characteristics Of Fuqu Fortified By Yeast Community Based On Jiangxiang Daqu, Wei Chunhui, Xu Ziheng, Ying Chao, Zhao Huan, Ren Zhiqiang

Food and Machinery

［Objective］ To solve the problems of single strain, limited fermentation capability, and weak environmental tolerance of yeast Fuqu.［Methods］ The yeast community of Jiangxiang Daqu is utilized as the strain source, and bran is used as the raw material to prepare a multi-yeast mixed Fuqu with fortified function. The preparation conditions of yeast Fuqu are optimized by single factor tests combined with orthogonal tests. Then, the acid, ethanol, and high-temperature resistance are systematically evaluated, while the microbial community structure is analyzed via internal transcribed spacer (ITS) rDNA high-throughput sequencing.［Results］ The optimal process parameters are a moisture content of 45%,an incubation time …