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Hybrid Machine And Deep Learning Framework For Secure Fog-Based Vanets, Hadeer Kamal, Amira Y. Haikal, Mahmoud M. Saafan Dec 2025

Hybrid Machine And Deep Learning Framework For Secure Fog-Based Vanets, Hadeer Kamal, Amira Y. Haikal, Mahmoud M. Saafan

Mansoura Engineering Journal

As a conventional implementation of intelligent transportation systems (ITS), a Vehicular Ad-hoc Network (VANET) facilitates smart communication between vehicle nodes and network infrastructures. Nevertheless, the distributed and dynamic characteristics of VANETs make them vulnerable to numerous cyberattacks like DOS and spoofing. Rapid detection of these threats is crucial. It is essential to respond promptly to mitigate threats before they impact vehicle coordination or infrastructure functionality. In this context, fog computing presents an effective solution. Transferring computational tasks from centralized cloud servers to fog nodes allows for quicker detection within the VANET environment. This research utilizes computational resources for swift and …


Integration Of Algae In Buildings: Challenges And Opportunities, Marwa Said Saad, Mostafa Refaat, Ashraf Nessim Dec 2025

Integration Of Algae In Buildings: Challenges And Opportunities, Marwa Said Saad, Mostafa Refaat, Ashraf Nessim

Mansoura Engineering Journal

Buildings produce 40% of energy-associated CO2 emissions and 35% of worldwide energy use which makes it one of the main factors of global warming. The use of bioactive materials on building façades is a creative way to address the aforementioned problems. The biotechnical potential of microalgae architecture to achieve net-zero energy architecture while simultaneously advancing ecological sustainability and occupant well-being has drawn attention. This paper aims to analyzing and investigating techniques of integration of algae in architecture showing the challenges and opportunities of this implementation. Analytical and Comparative analytical method were used in this paper, Initially, a thorough literature review …


Face Value: A Computational Approach To Subjective Impressions Of Faces, Kevin Kpankou Dec 2025

Face Value: A Computational Approach To Subjective Impressions Of Faces, Kevin Kpankou

Undergraduate Research Symposium

Various computational models of first impressions have been developed to uncover the mechanisms driving these judgments. However, the implicit notion of a singular ``human'' often overlooks meaningful individual differences in beliefs, attitudes, and associations, as well as culturally grounded group-level constructs. In this paper, we extend Cultural Consensus Theory (CCT) to estimate culturally shared beliefs about faces by incorporating latent constructs structured around interpretable facial features extracted via computer vision algorithms. We apply our model to a large-scale dataset of people’s first impressions of faces. Our approach reveals a robust mapping between facial features and culturally constructed impressions, allowing us …


Targeting Pancreatic Cancer With Rapamycin-Loaded Gold Nanoparticles Conjugated To Egf Peptide: Studies On Cytotoxicity And Uptake Mechanisms Aided By Proteomic Analysis, Adeolu Oluremi Dec 2025

Targeting Pancreatic Cancer With Rapamycin-Loaded Gold Nanoparticles Conjugated To Egf Peptide: Studies On Cytotoxicity And Uptake Mechanisms Aided By Proteomic Analysis, Adeolu Oluremi

Theses and Dissertations

Pancreatic cancer remains one of the most lethal malignancies, with a five-year survival rate below 5%. While rapamycin (Rap) has demonstrated therapeutic potential, its clinical utility is hampered by systemic toxicity. To address this limitation, this study developed a targeted nanocarrier system using gold nanospheres (AuNPs) functionalized with the Epidermal Growth Factor Receptor (EGFR) peptide GE11 for the specific delivery of Rap to pancreatic cancer cells. It is to note that pancreatic cancer overexpresses EGFR. The AuNP–GE11–Rap conjugates were successfully synthesized and characterized using physico-chemical techniques, which confirmed peptide conjugation and drug loading. In vitro studies using PANC-1 cells demonstrated …


Indigenizing Aquaculture: In Pursuit Of An Integrated Multi-Trophic Aquaculture System For Enhanced Self-Sufficiency In Akaroa Harbour, Aotearoa New Zealand, Cali R. Trainor Dec 2025

Indigenizing Aquaculture: In Pursuit Of An Integrated Multi-Trophic Aquaculture System For Enhanced Self-Sufficiency In Akaroa Harbour, Aotearoa New Zealand, Cali R. Trainor

Capstone Collection

This study assessed the feasibility of integrating two pre-existing aquaculture activities in Akaroa Harbour, Aotearoa New Zealand into an integrated multi-trophic aquaculture (IMTA) system. The cultivation of giant kelp (Macrocystis pyrifera) presents an opportunity to improve the sustainability of Chinook salmon (Oncorhynchus tshawytscha) farming by introducing alternative food sources for salmon, which can be used to produce fish feed locally, enhancing the self-sufficiency of the community and boosting the holistic efficiency of the system. Macroinvertebrates residing in giant kelp wrack were collected, identified, and quantified to determine if any of the Macrocystis-associated specimens could serve as …


The Role Of Attention And Design In The Effectiveness Of Eco-Feedback Systems, Lyndsey Bentzoni Dec 2025

The Role Of Attention And Design In The Effectiveness Of Eco-Feedback Systems, Lyndsey Bentzoni

Best Integrated Writing

This paper examines the role of eco-feedback systems in promoting pro-environmental behaviors by providing users with easily accessible information about their resource consumption. While these systems have shown potential to reduce consumption, they can face difficulties in maintaining user engagement and achieving long-term behavior change. There are several key factors related to the design of feedback interfaces that influence the effectiveness of eco-feedback systems. Gamification has been proposed as an effective way to enhance user engagement but requires careful design to connect behaviors from the digital to the real space for learning transfer. Research on the long-term usage of these …


Best Integrated Writing Fall 2025 - Complete Edition, Wright State University School Of Humanities And Cultural Studies, Tracy Smith, Kristie Mckiernan Dec 2025

Best Integrated Writing Fall 2025 - Complete Edition, Wright State University School Of Humanities And Cultural Studies, Tracy Smith, Kristie Mckiernan

Best Integrated Writing

Best Integrated Writing includes excellent student writing from Integrated Writing courses taught at Wright State University.


Synteny To Snps: Comparative And Population Genomics Of Endemic Gila River Fishes, Alexander C. Cameron Dec 2025

Synteny To Snps: Comparative And Population Genomics Of Endemic Gila River Fishes, Alexander C. Cameron

Biology ETDs

Population genomic data are becoming an essential component of the best available science used by conservation professionals to make management decisions to preserve biodiversity in a rapidly changing world. The proliferation of high-throughput sequencing technologies has made generating genome scale data feasible for non-model organisms that are of conservation concern. In addition to applicability in conservation science, genomic data enable the exploration of basic questions related to species evolution. To that end, this body of research exemplifies the applied and basic utility of genomic data and is focused on two federally-protected minnows endemic to the Gila River basin, Loach Minnow …


Energy Input–Output Meta-Analysis Reveals Algal Diesel Struggles To Break Even, Michelle M. Arnold, David J. R. Murphy, Christopher L. Lant Dec 2025

Energy Input–Output Meta-Analysis Reveals Algal Diesel Struggles To Break Even, Michelle M. Arnold, David J. R. Murphy, Christopher L. Lant

Environment and Society Student Research

Algal biofuels have been investigated as an alternative to fossil fuels and first-generation biofuels for transportation in the United States since the 1970s. Yet after five decades of development, scalability and implementation remain limited—largely due to persistent barriers such as low biomass productivity, modest lipid yields, and energy-intensive processing methods. These technical challenges significantly constrain the feasibility of large-scale commercialization despite substantial research and investment. To evaluate progress toward commercial viability, this study harmonized energy inputs and outputs across 508 observations on the production of algal biofuel energy return on energy investment (EROEI) in the United States. While bioethanol achieves …


Methods For The Discovery Of Antibodies Toward Junin Virus Utilizing Yeast Surface Display, Jonathon E. Corral Dec 2025

Methods For The Discovery Of Antibodies Toward Junin Virus Utilizing Yeast Surface Display, Jonathon E. Corral

Doctoral Dissertations and Projects

The importance of this study is to perform antibody discovery as a means to derive novel preventative and therapeutic antibody countermeasures against the Junín virus, to alleviate the potential risk of a global pandemic. The purpose of this methodological study was to develop a protocol that utilizes yeast surface display to discover antibodies, from naïve human antibody libraries, that can bind the Junín Virus antigen. The methodology of yeast surface display employs the galactose-inducible GAL1 promoter to synthesize and express antibody proteins bound to the Aga2p and Aga1p and anchored to the cell wall by glycosyl phosphatidylinositol motif (GPI). The …


Dna Tetrahedron-Au Nps-Go For Enhanced Fluorescence Detection Of Serotonin, Zhang Yun, Wu Qiong, Gao Hui, Zhang Hua, Miao Xiang-Min Dec 2025

Dna Tetrahedron-Au Nps-Go For Enhanced Fluorescence Detection Of Serotonin, Zhang Yun, Wu Qiong, Gao Hui, Zhang Hua, Miao Xiang-Min

Journal of Food and Drug Analysis

Evidence had accumulated that serotonin (5-HT), a hormone and neurotransmitter, was not only involved in a variety of different physiological and central nervous system functions but also in the development and manifestation of psychiatric diseases. Here, DNA tetrahedron-Au NPs-GO for enhanced fluorescence sensor was designed for detecting serotonin. Au NPs-GO was used as a fluorescence quencher, while fluorophore-labeled DNA tetrahedron was used as a donor, resulting in fluorescence resonance energy transfer (FRET) from the fluorophore to Au NPs-GO (signal off). With the strong binding ability of aptamer to serotonin, S5 was released from Aptamer: S5 duplex, then reacted with the …


Targeting Chemical Carcinogenesis: Α-Pinene And D-Limonene As Chemopreventive Agents Against Nnk/ Tcdd- And Urethane-Induced Lung Tumorigenesis, Kuo-Ching Huang, Vui-Hyen Chew, Yu-Ying Chen, Huey-Jen Su, Hao-Ting Chang, Rong‑Jane Chen, Ying‑Jan Wang Dec 2025

Targeting Chemical Carcinogenesis: Α-Pinene And D-Limonene As Chemopreventive Agents Against Nnk/ Tcdd- And Urethane-Induced Lung Tumorigenesis, Kuo-Ching Huang, Vui-Hyen Chew, Yu-Ying Chen, Huey-Jen Su, Hao-Ting Chang, Rong‑Jane Chen, Ying‑Jan Wang

Journal of Food and Drug Analysis

Lung cancer is the leading cause of cancer-related deaths worldwide, driven by carcinogens such as tobacco-derived nitrosamines, dioxins, and urethane. This study evaluated the chemopreventive effects of two monoterpenes, α-pinene and D-limonene, using NNK/TCDD- and urethane-induced lung tumor models and A549 lung cancer cells. Both compounds significantly reduce tumor number and size, with enhanced effects when combined. Mechanistic studies revealed suppression of PRC1, β-catenin, and c-Myc, alongside activation of p53, Bax, and caspase-3, indicating inhibition of the PRC1—Wnt/β-catenin pathway and induction of apoptosis. These findings suggest α-pinene and D-limonene as safe, promising agents for lung cancer chemoprevention.


Cordycepin In Cancer Therapy: A Bibliometric Analysis And Review Of Mechanisms, Zhiwei Ouyang, Yufei Zhang, Jianghan Ning, Yayi Tu, Bin He Dec 2025

Cordycepin In Cancer Therapy: A Bibliometric Analysis And Review Of Mechanisms, Zhiwei Ouyang, Yufei Zhang, Jianghan Ning, Yayi Tu, Bin He

Journal of Food and Drug Analysis

Cordycepin (3’-deoxyadenosine), a major bioactive component derived from fungi of the genus Cordyceps, has garnered significant attention in recent years for its potent antitumor properties. Drawing on literature indexed in the Web of Science Core Collection from 2004 to 2025, this study employs bibliometric tools―specifically CiteSpace and VOSviewer―to systematically examine developmental trends, research hotspots, and emerging frontiers in the field of cordycepin-related cancer research. The analysis maps a shift in focus from early-stage pharmacological validation to more advanced investigations into molecular mechanisms, with particular emphasis on cell cycle regulation. Keyword burst analysis highlights bursts in terms such as “apoptosis,” “cell …


Cv: Sarah Powers (Biology), Sarah Powers Dec 2025

Cv: Sarah Powers (Biology), Sarah Powers

Biology Department Faculty Curricula Vitae

No abstract provided.


Clerodendrum Kelli (Lamiaceae), A New Species From Carranglan, Nueva Ecija, Philippines, And Lectotypification Of Clerodendrum Mindorense, David Justin R. Ples, Jacek Patykowski, John Charles A. Altomonte, Adriene B. Tobias, Rene Alfred Anton Bustamante Dec 2025

Clerodendrum Kelli (Lamiaceae), A New Species From Carranglan, Nueva Ecija, Philippines, And Lectotypification Of Clerodendrum Mindorense, David Justin R. Ples, Jacek Patykowski, John Charles A. Altomonte, Adriene B. Tobias, Rene Alfred Anton Bustamante

Biology Faculty Publications

Clerodendrum kelli (Lamiaceae), a new species from the montane forests of Carranglan, Nueva Ecija, Philippines, is described and illustrated. The species shares morphological features with Clerodendrum quadriloculare and C. mindorense, but differs in inflorescence structure, corolla tube length, and calyx shape and color. A lectotype for C. mindorense is also designated. Preliminary conservation assessment places C. kelli in the Critically Endangered category (CR B1ab(iii)+2ab(iii)). This study contributes to Philippine plant biodiversity knowledge and informs conservation strategies.


Effects Of Compound Protease Fermentation On The Industrial-Scale Production Of Very High Gravity Brewing Beer, Zongming Chang, Guangfeng Liu, Li Fang, Jiajie Zhang, Hua Yin Dec 2025

Effects Of Compound Protease Fermentation On The Industrial-Scale Production Of Very High Gravity Brewing Beer, Zongming Chang, Guangfeng Liu, Li Fang, Jiajie Zhang, Hua Yin

Journal of Food Science and Biotechnology

[Objective ] This study aims to address the technical problems of insufficient nitrogen content and an imbalanced amino acid composition in very high gravity wort produced with high-level syrup adjuncts.[Method ] This study investigated the effect of protease-assisted saccharification on wort protein composition.Then,a comprehensive evaluation was conducted on the brewing performance during 22 ° P very high gravity beer production.[Result ] During the proteolysis stage of saccharification,a synergistic addition of neutral protease and flavourzyme protease at a mass ratio of 6∶4 enabled efficient directed hydrolysis of malt proteins.In the wort,the α-amino nitrogen mass concentration was increased by 30%,while the mass …


Rapid Detection Of Aflatoxin B 1 Contamination Levels In Peanuts Based On Dual-Band Hyperspectral Imaging And Machine Learning, Lu Ren, Shuyue Shen, Wanrong Shi, Chuang Rui, Maofeng Dong, Tingting Li Dec 2025

Rapid Detection Of Aflatoxin B 1 Contamination Levels In Peanuts Based On Dual-Band Hyperspectral Imaging And Machine Learning, Lu Ren, Shuyue Shen, Wanrong Shi, Chuang Rui, Maofeng Dong, Tingting Li

Journal of Food Science and Biotechnology

[Objective ] This study aims to achieve rapid detection of AFB 1 contamination levels in peanuts through dual-band hyperspectral imaging and machine learning.[Method ] A peanut AFB 1 contamination level warning model was constructed via a dual-band hyperspectral imaging system consisting of visible near-infrared spectroscopy at 400~1 000 nm and short-wave near-infrared spectroscopy at 900~1 700 nm,combined with machine learning.Peanut samples with four contamination levels were collected,in which the mass fraction of AFB 1 was determined by ultra-high performance liquid chromatography-tandem mass spectrometry.The effects of four pretreatment methods [first derivative,second derivative,multiplicative scatter correction,and standard normal variate (SNV )],two characteristic wavelength …


Protective Effects Of Ovalbumin-Sodium Carboxymethylcellulose Colloidal Particles On Fish Oil Emulsions, Weichun Pan, Ya Wen, Huabin Liao, Feina Gu, Qiuping Zhang, Fuge Niu Dec 2025

Protective Effects Of Ovalbumin-Sodium Carboxymethylcellulose Colloidal Particles On Fish Oil Emulsions, Weichun Pan, Ya Wen, Huabin Liao, Feina Gu, Qiuping Zhang, Fuge Niu

Journal of Food Science and Biotechnology

[Objective ] This study aimed to investigate the impact of curcumin-loaded ovalbumin (OVA )-carboxymethylcellulose (CMC ) (OVA -CMC-Cur ) colloidal particles on the stability of fish oil emulsions.[Method ] The OVA -CMC-Cur colloidal particles were prepared with OVA and CMC at varying mass ratios and pH.The colloidal particles were then used as the stabilizer to prepare fish oil emulsions.The structural characteristics,stability,and digestive behaviors of the emulsions were investigated.[Result ] At the OVA -CMC mass ratio of 2∶1,the OVA -CMC-Cur particle-stabilized fish oil emulsions exhibited smaller particle sizes.These particles formed a dense,compact interfacial film at the oil-water interface,resulting in more uniform …


Therapeutic Mechanisms Of A Lilium Brownii -Plantago Asiatica L.Compound Solid Beverage Against Hyperuricemia, Feng Li, Jiawei Huang, Xiaowen Guo, Lulu Jiang, Hongyan Zhao, Qiang Li, Chenxiang Sun, Shuying Feng, Qianqian Wang Dec 2025

Therapeutic Mechanisms Of A Lilium Brownii -Plantago Asiatica L.Compound Solid Beverage Against Hyperuricemia, Feng Li, Jiawei Huang, Xiaowen Guo, Lulu Jiang, Hongyan Zhao, Qiang Li, Chenxiang Sun, Shuying Feng, Qianqian Wang

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the ameliorative effects of a Lilium brownii -Plantago asiatica L.compound solid beverage on hyperuricemia and to elucidate its potential mechanisms.[Method ] Network pharmacology was employed to screen the active constituents,potential targets,and related pathways.Key targets were identified based on a protein-protein interaction (PPI) network.Molecular docking was conducted between the key proteins and active constituents.An animal experiment was performed to validate the effects of the compound solid beverage on serum uric acid levels,renal function indices,activities of uric acid-producing enzymes,and the levels of renal urate transporters.[Result ] Network pharmacology analysis indicated that Lilium brownii and Plantago …


Research Progress In Enrichment Of Trace Elements Zinc And Selenium In Pleurotus Spp., Jing Zhang, Mengmeng Xu, Liting Zhao, Cui Li, Lei Chen, Song Ming, Zhongyang Ding Dec 2025

Research Progress In Enrichment Of Trace Elements Zinc And Selenium In Pleurotus Spp., Jing Zhang, Mengmeng Xu, Liting Zhao, Cui Li, Lei Chen, Song Ming, Zhongyang Ding

Journal of Food Science and Biotechnology

Zinc and selenium are essential trace elements for maintaining human normal physiological function.Deficiency of zinc or selenium can result in immune dysfunction,chronic diseases such as cardiovascular diseases and hyperlipidemia,and even increase the risk of cancer.The human body cannot independently synthesize these trace elements,which can only be obtained from the daily diet.However,natural food sources have low content of zinc and selenium,which is insufficient to meet the bod y’s needs.Therefore,developing fortified foods with trace nutrients is an important approach to meeting residents ’ requirements.Edible fungi serve as natural carriers for enriching trace elements and ideal dietary supplements.Pleurotus spp.,as a typical example,not only …


Construction And Optimization Of An Efficient Tyrosol Biosynthesis System In Escherichia Coli, Yizhe Xiong, Shougang Wang, Zhiming Rao, Meijuan Xu Dec 2025

Construction And Optimization Of An Efficient Tyrosol Biosynthesis System In Escherichia Coli, Yizhe Xiong, Shougang Wang, Zhiming Rao, Meijuan Xu

Journal of Food Science and Biotechnology

[Objective ] An efficient biosynthetic system was constructed in Escherichia coli to achieve high-yield production of tyramine and its key derivative tyrosol.[Method ] Through the analysis of protein expression,enzymatic properties,and whole-cell catalysis results,tyrosine decarboxylase (TDC ) derived from Enterococcus faecalis was selected as the key enzyme for tyramine synthesis.Subsequently,tyramine oxidase (TYO ) was introduced to construct the TDC-TYO pathway for tyrosol production.Meanwhile,the LAAD-PDC pathway for tyrosol synthesis was established.A dual-enzyme co-expression system was established,and the effects of tandem enzyme order,production pathways,and key enzyme sources on tyrosol yield were analyzed.The recombinant strain B 1,carrying the plasmid pET 28a-EfTDC-TYO,was obtained through whole-cell …


Promoting The Synthesis Of Salicylic Acid In Escherichia Coli Based On Metabolic Engineering And Multi-Enzyme Cascade Strategy, Kai Wang, Xuewei Pan, Taowei Yang, Zhiming Rao Dec 2025

Promoting The Synthesis Of Salicylic Acid In Escherichia Coli Based On Metabolic Engineering And Multi-Enzyme Cascade Strategy, Kai Wang, Xuewei Pan, Taowei Yang, Zhiming Rao

Journal of Food Science and Biotechnology

[Objective ] The production of the aromatic compound salicylic acid (SA) is limited by its inhibitory effects on enzymes related to cellular energy metabolism.This study aims to develop a strategy for avoiding the impact of SA on enzymes related to cellular energy metabolism and increase the yield of SA.[Method ] A highly efficient Escherichia coli strain was designed to improve SA production by optimizing metabolic pathways and enzyme systems.[Result ] The genome-scale metabolic model GEMS 1515 was employed to predict the key bottle necks in the phosphoenolpyruvic acid (PEP) pathway and SA biosynthesis pathways.The SA yield was successfully increased from …


Fermentation Technology Of L-Lysine Production By Immobilized Corynebacterium Glutamicum, Qingguo Liu, Hanjie Ying, Wenjun Sun Dec 2025

Fermentation Technology Of L-Lysine Production By Immobilized Corynebacterium Glutamicum, Qingguo Liu, Hanjie Ying, Wenjun Sun

Journal of Food Science and Biotechnology

[Objective ] This study aimed to investigate the feasibility of the fermentation process for L-lysine production by immobilized Corynebacterium glutamicum.[Method ] Firstly,the effects of different volume fractions of seed medium on the fermentation stability of immobilized Corynebacterium glutamicum were examined.Subsequently,the impacts of immobilization carriers with different specifications on the fermentation performance were analyzed.Finally,a comparative analysis was conducted on the differences between the fermentation processes with free and immobilized Corynebacterium glutamicum.[Result ] The addition of 5% volume fraction of seed medium adequately sustained the growth and metabolic activities of Corynebacterium glutamicum.Higher volume fractions diverted carbon sources towards bacterial growth,thereby limiting product …


Reduction Of Methanol Content By Protoplast Fusion In Fruit Wine Yeast, Huixiang Zhang, Nan Lin, Jiaru Wu, Xin Cheng, Weiwu Liang, Shijun Yang Dec 2025

Reduction Of Methanol Content By Protoplast Fusion In Fruit Wine Yeast, Huixiang Zhang, Nan Lin, Jiaru Wu, Xin Cheng, Weiwu Liang, Shijun Yang

Journal of Food Science and Biotechnology

[Objective ] This study aims to breed a fruit wine yeast strain with low methanol production by protoplast fusion.[Method ] With yeast strains LAB and XR as parents,protoplast fusion was employed to breed the yeast strains for fruit wine fermentation with a low mass concentration methanol and a high volume fraction ethanol.The process of yeast protoplast preparation was optimized by single factor and orthogonal experiments with the formation rate,regeneration rate,and preparation rate of protoplasts as indicators.The parental protoplasts were inactivated by ultraviolet radiation and heating,and 35 g/dL PEG- 6000 was used for chemical fusion after inactivation.[Result ] The optimum conditions …


Effects Of Metschnikowia Pulcherrima And 24-Epibrassinolide On Postharvest Preservation Of Grapes, Jiayan Qu, Huiping Niu, Bowen Li, Xiao Wang, Lujun Wang, Zhumei Xi, Xuefei Wang Dec 2025

Effects Of Metschnikowia Pulcherrima And 24-Epibrassinolide On Postharvest Preservation Of Grapes, Jiayan Qu, Huiping Niu, Bowen Li, Xiao Wang, Lujun Wang, Zhumei Xi, Xuefei Wang

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the effects of different treatments on the postharvest preservation of grapes.[Method ] The impacts of 0.4 mg/L 24-epibrassinolide (EBR ),1×109 CFU/mL Metschnikowia pulcherrima,and their combination (YBR ) on postharvest storage of ‘Shine Muscat ’ grapes were evaluated.[Result ] Compared with the control (CK) group,EBR,Metschnikowia pulcherrima,and YBR treatments effectively reduced the water loss rate,maintained the firmness,and delayed the declines in protopectin and stem chlorophyll content of grape.In the later storage stage,YBR treatment delayed the decrease in flavonoid mass fraction,which was 12.95% higher than that of CK group.At the same time,YBR treatment reduced the mass …


Effects Of Single And Mixed Fermentation On Flavor , Composition , And Antioxidant Activity Of Soybean Whey, Taotao Wei, Qian Chen, Xiangjie Tao, Bin Tang, Fusheng Ren, Ping Hu Dec 2025

Effects Of Single And Mixed Fermentation On Flavor , Composition , And Antioxidant Activity Of Soybean Whey, Taotao Wei, Qian Chen, Xiangjie Tao, Bin Tang, Fusheng Ren, Ping Hu

Journal of Food Science and Biotechnology

[Objective ] This study aims to explore the impacts of single strain fermentation and mixed fermentation on the flavor,composition,and antioxidant activity of soybean whey.[Method ] Gas chromatography-ion mobility spectrometry (GC-IMS ) was coupled with E-nose and high-performance liquid chromatography (HPLC ) to a nalyze the flavor and function of soybean whey fermented by the single and mixed strains.[Result ] Compared with single-strain fermentation by Limosilactobacillus fermentum HJS- 1 or Kluyveromyces marxianus HJS- 2,mixed fermentation (Limosilactobacillus fermentum HJS- 1 + Kluyveromyces marxianus HJS- 2) effectively improved the levels of functional substances and antioxidant activity of soybean whey.Compared with non-fermented soybean whey,mixed …


Efficient Catalytic Synthesis Of 5-Methylpyrazine- 2-Carboxylic Acid By Recombinant Escherichia Coli, Luxi Gan, Heng Gong Dec 2025

Efficient Catalytic Synthesis Of 5-Methylpyrazine- 2-Carboxylic Acid By Recombinant Escherichia Coli, Luxi Gan, Heng Gong

Journal of Food Science and Biotechnology

[Objective ] This study aimed to improve the yield and conversion rate of 5-methylpyrazine- 2-carboxylic acid (MPCA ) through biocatalysis.[Method ] Escherichia coli (E.coli)was used as the model organism to construct an MPCA synthesis system,and the whole-cell catalytic reaction conditions of recombinant E.coli were optimized.In addition,the parental host strain was subjected to ultraviolet mutagenesis for the screening of host strains tolerant to high mass concentrations of 2,5-dimethylpyrazine (DMP ).[Result ] The recombinant mutagenized strain could completely convert 10 g/L DMP to MPCA within 12 h in a 10 mL reaction system,achieving a yield of 12.9 g/L and a corresponding molar …


Goat Milk Formula Powder Ameliorates Lipopolysaccharide-Induced Intestinal Inflammation, Yi Shan, Xiaoling Liu, Shumei Wang Dec 2025

Goat Milk Formula Powder Ameliorates Lipopolysaccharide-Induced Intestinal Inflammation, Yi Shan, Xiaoling Liu, Shumei Wang

Journal of Food Science and Biotechnology

[Objective ] This study aimed to investigate the effects of goat milk formula powder on lipopolysaccharide (LPS)-induced intestinal inflammation and barrier impairment in mice.[Method ] Histopathological observation,real-time quantitative PCR,and enzyme-linked immunosorbent assay were used to assess changes in intestinal inflammation levels and barrier function-related indicators in mice following intervention with goat milk formula powder.Additionally,the composition of the gut microbiota was analyzed to elucidate its potential mechanisms of action.[Result ] Goat milk formula powder alleviated intestinal histopathology and increased the number of goblet cells.Compared with the model group,the goat milk formula powder intervention reduced the mass fractions of pro-inflammatory cytokines TNF- …


Rab32c Mediates Osmoregulation And Calcium Homeoastasis In Dictyostelium Discoideum, Ashley Esparza Dec 2025

Rab32c Mediates Osmoregulation And Calcium Homeoastasis In Dictyostelium Discoideum, Ashley Esparza

Theses and Dissertations

Vesicular trafficking is a fundamental process essential to cell viability and dictates roles in both cellular and tissue function in multicellular organisms. To further understand various vesicular trafficking processes, our lab focuses on the moderators of these processes, Rab GTPases, using the model organism Dictyostelium discoideum. This dissertation focuses explicitly on the uncharacterized protein Rab32C, a homolog of human Rab32. While several Rab family members have been well defined, the functionality of Rab32C remains poorly understood. This project investigated both the subcellular localization and functional role of Rab32C with biochemical, imaging, and molecular assays. Results from this study provide evidence …


Fabrication Of Three-Dimensional Seaweed-Based Scaffolds And Evaluation Of Cell Attachment And Viability For Cultured Meat Applications, Srishti Tandon Dec 2025

Fabrication Of Three-Dimensional Seaweed-Based Scaffolds And Evaluation Of Cell Attachment And Viability For Cultured Meat Applications, Srishti Tandon

Honors Scholar Theses

Global demand for animal protein is increasing rapidly, underscoring the need for sustainable meat production. Cultured meat is a promising alternative to traditional meat production techniques, but it requires optimization before it can be implemented on a large scale. Scaffolds are an important component for making cultured meat, as they provide structural support for cell attachment and mimic the texture of meat. Scaffolds need to be biocompatible, mechanically supportive, and biochemically active to promote proper cell adhesion, proliferation, and differentiation. This project investigated brown seaweed extract (SWE) hydrogels as a scaffold for cultivated meat applications and compared their mechanical and …