Digital Commons Network^™

Mutations In The Pms1 Gene Associating With Ovarian Cancer, Elaine Vanterpool, Erin Hough

Student Posters

Ovarian cancer occurs when abnormal cells in the ovaries grow and divide uncontrollably, destroying healthy body tissue. Common symptoms of ovarian cancer include weight loss, fatigue, discomfort in the pelvic area, or changes in bowel habits. This study focuses on how ovarian cancer is related to the PMS1 gene. The PMS1 gene encodes MLH1, a protein involved in DNA mismatch repair (MMR) system. MMR helps connect errors that occur during DNA replication, preventing mutations and potential cancer development. When this gene is mutated, there is an increased risk of developing ovarian and other endometrial cancer. Bioinformaic softwares allow us to …

Differential Gene Coexpression In 16p11.2 Autism Spectrum Disorder, Joseph Patacsil

Senior Theses

Autism spectrum disorder (ASD) is a neurodevelopmental disorder that has been associated with several genetic factors. One of these factors is a mutation of the 16p11.2 region on chromosome 16, in which both deletions and duplications have been strongly associated with ASD. KCTD13 is a gene in the 16p11.2 gene locus that has recently been shown to influence brain development and is also associated with ASD. This study analyzes the differential gene expression and gene pathways of these different phenotypes. KCTD13 deletion had a significant up-regulation effect on genes and shares similar pathways to the 16p11.2 duplication mutation. Mutations in …

Impact Of Msx1 And Irf6 Gene Variants On Orofacial Cleft And Facial Development, Elaine Vanterpool, Lisa Banks

Student Posters

A cleft lip or palate is a congenital anomaly that occurs when a baby's mouth doesn’t form properly during pregnancy. It happens when the tissues that shape the upper lip or roof of the mouth fail to join during development. It is among the most common birth defects associated with genetic conditions or syndromes. Orofacial cleft can be caused by a combination of genes and other factors like the mother's exposure to environmental things, diet, and medications during pregnancy. Examining the genes involved and making connections that provide insight into the improper formations of the maxillary tissues during fetal stages …

Effects Of Cell-Free Supernatants Of Commercial Yogurt Products On Salmonella Survival, Juliet Durant, Akayla King

Student Posters

Salmonellosis remains a global public health challenge and Salmonella enterica serovar Enteritidis and Typhimurium are the primary causes. Salmonella virulence mechanisms involve attachment and invasion of intestinal epithelial cells. Gut microbiota and probiotics that contain Lactobacillus species have been shown to prevent Salmonella infection. Yogurt is a commonly consumed probiotic product containing Lactobacillus species. Yogurts made under laboratory conditions and the cell-free supernatants have been shown to inhibit Salmonella growth. The many commercial yogurt products on the market may vary in antimicrobial components based on production methods. Therefore, the present study aimed to evaluate the survival of 5 log CFU/mL …

Title: An Analysis Of Hmbs Variants Associated With Acute Intermittent Porphyria, Elaine Vanterpool, Jason Kahari

Student Posters

HMBS, also known as hydroxymethylbilane synthase, is a gene that is responsible for providing instructions for making the enzyme named hydroxymethylbilane synthase. This enzyme is involved in the creation of a molecule called heme. Heme plays an important part in all of the body’s vital organs. It is important to all organs however, it is most prevalent in the blood, bone marrow, and liver. Heme is essential in making iron-containing proteins called hemeproteins. To produce heme, an eight-step process must occur that requires 8 different enzymes. “Hydroxymethylbilane synthase is responsible for the third step in this process, which combines four …

The Association Of Abca3 Variants In Hypertension Pathogenesis, Elaine Vanterpool, Jea Joseph

Student Posters

Hypertension affects around 48.1% of people living in the United States. This number of adults with hypertension between the ages of 30-70 has increased from 650 million to 1.28 billion in the last 30 years. Hypertension is characterized as a condition that affects the arteries of the blood. High blood pressure and high cholesterol have a huge correlation to each other. When the body cannot rid cholesterol from the blood stream, it can deposit along artery walls and clog them, making blood flow harder and increases blood pressure. ABCA3 is a member of the ATP binding cassette family of proteins …

An Analysis Of Oas1 Variants Associated In Diabetes Mellitus, Elaine Vanterpool, Ashley Tannis

Student Posters

Type 1 diabetes is caused by a combination of multiple genetic and environmental factors that precipitate autoimmune destruction of the insulin-producing β-cells of the pancreas. Individuals with type 1 diabetes have higher frequencies of OAS1 GG and GA genotypes than nondiabetic's.OAS1, an interferon-induced gene, also known by its official name, 2'-5'-oligoadenylate synthetase 1, encodes proteins that play a crucial role in innate cellular antiviral response which prevents viral replication by activating the adaptive immune system to erase the virus trying to spread. OAS1 is also involved in additional cellular processes such as cell growth and cell death. This gene is …

Age-Associated Proteins Explain The Role Of Medial Temporal Lobe Networks In Alzheimer’S Disease, Adam Turnbull, Yejin Kim, Kai Zhang, Xiaoqian Jiang, Zihuai He, Victor W Henderson, F Vankee Lin

Faculty, Staff and Student Publications

The structural connectivity (SC) of the medial temporal lobe and its associated cortical anterior temporal and posterior medial networks (MTL-AT-PM) is linked to pathologies and memory decline in Alzheimer's disease (AD). However, neuroimaging analyses cannot tell us how SC changes occur in AD at the molecular level and do not provide a means of intervening to slow/prevent pathology-related changes in MTL-AT-PM SC. The current study aimed to understand how and where AD-related changes occur within MTL-AT-PM using proteomics. We used a 4-step approach in 101 older adults from a local sample, aiming to understand how proteins and SC in combination …

Investigation Of The Hdl Molecular Architecture Before And After Exercise Training., Prasun Kumar Dev

Theses and Dissertations

The functional characteristics of high-density lipoprotein (HDL) particles are influenced by their structure, including their molecular composition (e.g. HDL lipidome and proteome). However, the effects of exercise training on HDL molecular composition and its relationship with HDL-related traits are largely unknown. In this ancillary study, we investigated the HDL lipidome and proteome of 154 adults before and after 20 weeks of exercise training in the HERITAGE Family Study. The HDL-sized plasma fraction was isolated utilizing FPLC-SEC, followed by untargeted lipidomic and proteomic analysis using LC/MS. We identified 341 known lipid species while 409 HDL associated proteins. Clinical and functional related …

Drought And Herbivory Drive Physiological And Phytohormonal Changes In Soybean (Glycine Max Merril): Insights From A Meta-Analysis, Manish Gautam, Rupesh Kariyat

Entomology and Plant Pathology Faculty Publications and Presentations

With climate change, abiotic and biotic stresses such as drought and herbivory are predicted to further diminish agricultural productivity. Soybean (Glycine max [L.] Merrill), a crop of global economic importance, is vulnerable to both. However, the interactive effects of drought and herbivory on soybeans haven't been explored, especially through the lens of physiological and phytohormonal changes. To address this, we conducted a meta-analysis from 114 published studies, further reduced to 31 after removing redundancy, with data on physiological (photosynthesis and stomatal conductance) and phytohormonal traits (jasmonic acid [JA] and salicylic acid [SA]) of soybeans under drought, herbivory, and their combination. …

Investigating Sk-3 Based Spore Killing In Neurospora Crassa Through Deletion Analysis Of Dna Intervals I383 And I394, Paulina Paulikas

Senior Theses – Biological Sciences

Neurospora fungi are found around the world. The species N. crassa is a popular model for use in genetics research. N. crassa produces sexual spores, called ascospores, during mating between strains of opposite mating types. N. crassa also produces spore sacs called asci, and each ascus typically contains eight viable ascospores. However, some Neurospora fungi carry selfish genetic elements called Spore killers, and when a strain carrying a Spore killer mates with a spore killing-susceptible strain, asci contain four black viable ascospores and four white inviable ascospores. In this project, I investigated a Spore killer called Sk-3. To act as …

Examining The Mechanism Of Spore Sacs Undergoing Sk-3-Based Spore Killing After Deletion Of Neurospora Crassa Dna Intervals I382 And I400, Makenna Klann

Senior Theses – Biological Sciences

Neurospora crassa is a well-known model organism for studying eukaryotic genetics, particularly non-Mendelian inheritance mechanisms such as meiotic drive. In N. crassa, meiotic drive can be observed in fungal spore killing, where Spore killer-3 (Sk-3) is a selfish genetic element transmitted to offspring through spore killing. Sk-3 is thought to contain two principal components: a killer (poison) gene and a resistance (antidote) gene. While the resistance gene (rsk) has been identified, the killer gene remains unknown. Building on previous research that identified a 1.3 kb DNA interval (i350) essential for Sk-3-based spore killing, I …

Determining The Effects That Deletion Of I386 And I408 Have On Sk-3-Type Spore Killing, Kole Damkoehler

Senior Theses – Biological Sciences

Neurospora crassa is a genus of fungus that exhibits a phenomenon called Sk-3 spore killing. Sk-3 spore killing occurs when an Sk-3 killer strain mates with an Sk-3 sensitive strain, and it results in the death of half of the offspring. A DNA interval called i350, located on N. crassa Chromosome III, has previously been identified as critical for spore killing. Here, to obtain a more detailed understanding of this DNA interval, the effects of the deletion of related DNA intervals i386 and i408 on spore killing has been studied. Deletion of i386 resulted in no disruption of spore …

Three Reference Genomes For Freshwater Diatom Ecology And Evolution, Wade R. Roberts, Andrew J. Alverson

Biological Sciences Faculty Publications and Presentations

Diatoms are an important component of marine and freshwater ecosystems. Although the majority of described diatom species live in freshwater systems, genome sequencing efforts have focused primarily on marine species. Genomic resources for freshwater species have the potential to improve our understanding of diatom ecology and evolution, particularly in the context of major environmental shifts. We used long- and short-read sequencing platforms to assemble reference genomes for three freshwater diatom species, all in the order Thalalassiosirales, which are abundant in the plankton of oceans, lakes, reservoirs, and rivers worldwide. We targeted three species that cover the breadth of phylogenetic diversity …

Warm-Ischemia And Cold Storage Induced Modulation Of Ferroptosis Observed In Human Hearts Donated After Circulatory Death And Brain Death, Shiyi Li, Katherine V Nordick, Abdussalam E Elsenousi, Rishav Bhattacharya, Randall P Kirby, Adel M Hassan, Camila Hochman-Mendez, Todd K Rosengart, Kenneth K Liao, Nandan K Mondal

Faculty, Staff and Students Publications

We investigated ferroptosis, a type of programmed cell death mechanism, in human hearts donated after brain death (DBD) and those donated after circulatory death (DCD), focusing on warm ischemia time (WIT) and cold storage. A total of 24 hearts were procured, with six from the DBD group and 18 from the DCD group. The DCD group was divided into three subgroups, each containing six hearts, based on different WITs of 20, 40, and 60 min. All procured hearts were placed in cold storage for up to 6 h. Left ventricular biopsies were performed at 0, 2, 4, and 6 h. …

Unveiling Racial Disparities In Prostate Cancer Using An Integrative Genomic And Transcriptomic Analysis, Abdalla Elbialy, Akshay Sood, Shang-Jui Wang, Peng Wang, Ahmed Fadiel, Anil V Parwani, Steven Huang, Gennady Shvets, Nagireddy Putluri, Jenny Li, Xuefeng Liu

Faculty, Staff and Students Publications

Prostate cancer exhibits significant racial disparities, with African American (AA) individuals showing ∼64% higher incidence and 2.3 times greater mortality rates compared to their Caucasian (CA) counterparts. Understanding the complex interplay of genetic, environmental, lifestyle, socioeconomic, and healthcare access factors is crucial for developing effective interventions to reduce this disproportionate burden.

This study aims to uncover the genetic and transcriptomic differences driving these disparities through a comprehensive analysis using RNA sequencing (RNA-seq) and exome sequencing of prostate cancer tissues from both Black and White patients.

Our transcriptomics analysis revealed enhanced activity in pathways linked to immune response and cellular interactions …

Culex Quinquefasciatus Mosquitoes In Abu Dhabi Emirate: Assessing Deltamethrin Insecticide Resistance And Associated Microbial Communities, Amgdsayed Ismat Ali

Theses

Culex quinquefasciatus is a major vector of West Nile virus, lymphatic filariasis, and arboviruses, which represent public health risk. Widespread application of pyrethroid insecticides has increased resistance in mosquito populations threatening the effectiveness of control strategies. This study aims to (1) assess deltamethrin resistance in Culex quinquefasciatus populations across the Abu Dhabi Emirate, (2) characterize the microbial communities associated with these mosquitoes.

Mosquitoes from sites around the Abu Dhabi Emirate were collected and genomic DNA extracted for molecular analysis. PCR and allele-specific primers were used to detect knockdown resistance (kdr) mutations in the sodium channel gene, classifying mosquitoes as susceptible …

Know Where You Go: Infestation Dynamics And Potential Distribution Of Two Bed Bug Species (Hemiptera: Cimicidae) In Africa, Dennis M. Mbuta, Bonoukpoè M. Sokame, Fathiya M. Khamis, Komivi S. Akutse

All Peer-Reviewed Publications

Bed bugs (Hemiptera: Cimicidae) are blood-feeding insects closely linked with humans and animals, causing discomfort, and posing potential threats as disease vectors. This study focuses on Cimex lectularius (common bed bug) and Cimex hemipterus (tropical bed bug), the two key species identified in Africa. Cimex lectularius infests human habitats, while C. hemipterus, more common in Africa, infests diverse habitats, including bat colonies. In our study, we investigated the infestation dynamics and distribution of bed bugs in Africa, when considering climate, habitat, and host availability using system dynamics and ecological niche modelling techniques. System dynamics modelling analyses in Kenya revealed varied …

The Cellular Trafficking And Targeting Of Angiotensin-Converting-Enzyme-2 (Ace2) And Neutral-Amino-Acid-Transporter (B0at1) Variants: Implications For The Pathogenesis Of Associated Diseases And Therapy, Nesreen Fayyad Alkhofash

Dissertations

Angiotensin-converting enzyme 2 (ACE2) and the neutral amino acid transporter B0AT1, encoded by SLC6A19, are membrane proteins with pivotal roles in human physiology. ACE2 is involved in regulating blood pressure and serves as the cellular entry receptor for SARS-CoV-2, while B0AT1 facilitates amino acid absorption in the intestine. The interplay between ACE2 and B0AT1, particularly their physical interaction and co-expression in the intestine, underscores their relevance in both normal physiology and disease. Dysregulation of these proteins has been implicated in conditions such as hypertension, and Hartnup disease and they have been usurped by SARS-CoV-2 to cause COVID-19. Despite their …

Exploring Date Pomace As A Functional Ingredient In Bread: Nutritional And Functional Properties, Alaa Almoumen

Dissertations

This study investigated the incorporation of desugared date fruit pomace into wheat bread rolls and evaluated its effects on dough handling, bread quality, staling behavior, sensory acceptability, and nutritional composition. The research was conducted in three experimental phases: (i) development and characterization of a high-fiber dietary ingredient (HFDI) from date pomace, including its physicochemical and functional properties; (ii) bread fortification and quality assessment, where wheat flour was partially replaced with 0–20% HFDI to evaluate its effects on dough rheology, bread texture, color, moisture retention, shelf life, and consumer acceptance; and (iii) nutritional and physiological evaluation of the fortified breads, including …

From Microplastic Impact To Biodegradation Solutions: Investigating Microplastic Pollution, Microbial Responses, And Efficacy Of Synthetic Microbial Consortia In Plastic Degradation, Rener Sahot De Jesus

Dissertations

Plastics, including microplastics, pose significant environmental threats due to their widespread presence and harmful ecological effects. This dissertation present three distinct, yet interconnected studies. First, a study on microplastic contamination in urban recreational parks was conducted in Al Ain, United Arab Emirates. Results showed that low-density microplastics were present in 87% of soil samples, with spectroscopic analysis identifying synthetic polymers such as polyethylene and polypropylene. Additionally, these microplastics were found to negatively affect soil pH and moisture levels, emphasizing the need for enhanced monitoring and management strategies. Second, the effects of ground microplastics on Photobacterium leiognathi strains, a marine luminous …

Expanding The Lexa Bacterial Two-Hybrid System For Human Protein-Protein Interaction Screening: Application To Tumor Suppressor Par-4, Rebecca M. Richardson

Chemistry & Biochemistry Theses & Dissertations

Protein-protein interactions (PPI) are fundamental to cellular function, influencing processes such as signaling, structural organization, and disease progression. Disruptions in PPIs are implicated in cancer and neurodegenerative diseases, highlighting their importance as therapeutic targets. However, traditional methods for studying human PPIs, such as yeast two-hybrid and co-immunoprecipitation mass spectrometry, are costly and technically demanding. This dissertation explores the LexA-based bacterial two-hybrid (LexA-B2H) system as a cost effective and accessible alternative for investigating human PPIs.

This study focuses on the interaction between glucose-regulated protein 78 (GRP78) and cleaved prostate apoptosis response-4 (cl-Par-4), a tumor suppressor protein involved in apoptosis and neurodegenerative …

Anthropogenic Influences On Parasitoid Wasps’ Biocontrol Of Invasive Insect Pest Species In Africa, Fathiya M. Khamis, Inusa J. Ajene

All Peer-Reviewed Publications

Sustainable agriculture is crucial to ensuring food and nutritional security and improving livelihoods of millions across Africa. However, agricultural production is constrained by native and invasive pests. Indiscriminate insecticide use is often the first line of action to manage these pests, harming human and animal health, and impacting biodiversity. Thus, shifting to more ecologically sustainable alternatives is key to biodiversity conservation and sustainable agriculture. Biological control is a core component of integrated pest management strategies, and natural enemies, like parasitoid wasps, are considered one of the most environmentally sustainable means of managing pests. However, the Anthropocene plays a significant role …

4-Ethylacetophenone From Potato Plants Repels Phthorimaea Operculella And Inhibits Oviposition: A Sustainable Management Strategy, Xinyu Ma, Junjie Yan, Guangyuan Su, Fathiya M. Khamis, Athanase Hategekiman, Yulin Gao

All Peer-Reviewed Publications

The potato tuber moth, Phthorimaea operculella (Zeller), is an important pest of potato crops capable of boring into and consuming the potato tubers. Although chemical insecticides are employed to control P. operculella populations, their efficacy is frequently limited, raising environmental concerns. Host plant volatiles are important allelochemicals that can be exploited for the management of pests. In our previous studies, we identified five volatile organic compounds (3-ethylacetophenone, 4-ethylacetophenone, geranylacetone, ethylbenzene, and 4-hydroxy-4-methyl-2-pentanone) as allelochemicals potentially influencing the oviposition behavior of P. operculella. Using electroantennography (EAG) and behavioral assays, we found that 4-ethylacetophenone significantly repelled P. operculella at all tested concentrations …

Nanosilica Supplementation In Tomato Increases Oviposition On Stems And Caterpillar Mortality In The Tomato Pinworm, Kokou R. Fiaboe, Fathiya M. Khamis, Xavier Cheseto, Abdullahi A. Yusuf, Baldwyn Torto

All Peer-Reviewed Publications

Silicon-induced responses play a key role in plant defense against herbivory, though the underlying mechanisms remain underexplored. In this study, we examined how mesoporous silica nanoparticles (MSNs) affect tomato (Solanum lycopersicum) defense against an invasive and highly destructive lepidopteran herbivore, the tomato pinworm (TPW), Phthorimaea absoluta. In tomato plants supplemented with MSN, prior exposure to TPW oviposition shifted subsequent egg-laying from a preference for leaves to an even distribution between stems and leaves. This shift was not observed in nonsilicon-supplemented plants. Prolonged oviposition triggered pigmentation in the basal cells of type I glandular trichomes on the stems of silicon-supplemented plants. …

Development Of A Noninvasive Genotyping-In-Thousands (Gtseq) Panel For Long-Term Conservation Of Western Great Lakes Gray Wolves (Canis Lupus), Samuel D. Hervey, Bridgett M. Vonholdt, Mark C. Romanski, Tyler J. Wheeldon, Brent R. Patterson, Kristin Brzeski

Michigan Tech Publications

The application of noninvasive genetic methods toward the field of conservation has increased our understanding of many wildlife populations that are difficult to sample, allowing for better management. In molecular ecology, the use of noninvasive sampling became widely feasible with the advent of microsatellites, a highly polymorphic, short-length marker that could be genotyped from low-quality DNA sources. Despite decades of use, many microsatellite panels continue to suffer from high genotyping error rates, allelic dropout, and limited reproducibility across laboratories. To address these issues, single nucleotide polymorphisms (SNPs) offer advantages such as lower genotyping error rates, avoidance of allelic dropout due …

Low Dissolved Oxygen Operation Enhances Biological Phosphorus Removal In Wastewater Treatment, Haley Morgan

Civil & Environmental Engineering Theses & Dissertations

Biological phosphorus (bio-P) removal occurs when bacteria are exposed to anaerobic/aerobic conditions that stimulate polyphosphate-accumulating organisms (PAOs) to uptake phosphorus. In most treatment plants, dissolved oxygen (DO) concentrations in the aeration tanks are commonly at or above 2.0 mg O₂/L, which is often referred to as the most optimal aeration condition for bio-P. If bio-P can happen efficiently at lower DO (< 0.3 mg O₂/L), then the energy demand of aeration can be decreased appreciably. This research was conducted in a biological nutrient removal (BNR) pilot plant to assess the performance of bio-P at progressively lower DO operation. This …

Development Of Flexible Lactate-Specific Molecularly Imprinted Polymers Based On Laser-Induced Graphene Electrochemical Biosensors For Disease Diagnosis, Christopher Animashaun

Biomedical Engineering Theses & Dissertations

Lactate is a critical biomarker linked to conditions such as sepsis, ischemia, cancer, and heart failure. Current lactate detection methods remain invasive, costly, and unsuitable for real-time, continuous monitoring. This study presents the development of a flexible, non-enzymatic electrochemical biosensor based on laser-induced graphene (LIG) electrodes modified with gold nanoparticles (AuNPs) and molecularly imprinted polymers (MIPs) for highly selective and sensitive lactate measurement. The integration of AuNPs enhanced surface conductivity and provided nanoscale heterogeneity, facilitating superior signal amplification. The optimized LIG/AuNP/MIP sensor achieved an extensive dynamic detection range from 0.1 μM to 1000 μM, with an outstanding detection limit of …

When Can We Detect Lianas From Space? Toward A Mechanistic Understanding Of Liana-Infested Forest Optics, Stefan A. Schnitzer

Biological Sciences Faculty Research and Publications

Lianas, woody vines acting as structural parasites of trees, have profound effects on the composition and structure of tropical forests, impacting tree growth, mortality, and forest succession. Remote sensing could offer a powerful tool for quantifying the scale of liana infestation, provided the availability of robust detection methods. We analyze the consistency and global geographic specificity of spectral signals—reflectance across wavelengths—from liana-infested tree crowns and forest stands, examining the underlying mechanisms of these signals. We compiled a uniquely comprehensive database, including leaf reflectance spectra from 5424 leaves, fine-scale airborne reflectance data from 999 liana-infested canopies, and coarse-scale satellite reflectance data …

Single-Nucleus Transcriptional Profiling Of The Placenta Reveals The Syncytiotrophoblast Stress Response To Covid-19, Rachel A Keuls, Scott A Ochsner, Mary B O'Neill, Diana R O'Day, Akihiko Miyauchi, Kadeshia M Campbell, Natalie Lanners, Jeffery A Goldstein, Connor Yee, Neil J Mckenna, Ronald J Parchem, Jacqueline G Parchem

Faculty, Staff and Students Publications

Background: COVID-19 in pregnancy is associated with placental immune activation, inflammation, and vascular malperfusion, but its impact on syncytiotrophoblast biology and function is unclear.

Objective: This study aimed to determine the effects of maternal COVID-19 on placental syncytiotrophoblasts using single-nucleus transcriptional profiling and to compare placental stress responses in COVID-19 and preeclampsia.

Study design: For transcriptional characterization of syncytiotrophoblasts, we used the single-nucleus RNA sequencing platform, single-cell combinatorial indexing RNA sequencing (sci-RNA-seq3), to profile placental villi and fetal membranes from unvaccinated patients with symptomatic COVID-19 at birth (n = 4), gestational age-matched controls (n = 4), and a case of …