Digital Commons Network^™

Distinct Adipose Progenitor Cells Emerging With Age Drive Active Adipogenesis, Guan Wang, Gaoyan Li, Anying Song, Yutian Zhao, Jiayu Yu, Yifan Wang, Wenting Dai, Martha Salas, Hanjun Qin, Leonard Medrano, Joan Dow, Aimin Li, Brian Armstrong, Patrick T Fueger, Hua Yu, Yi Zhu, Mengle Shao, Xiwei Wu, Lei Jiang, Judith Campisi, Xia Yang, Qiong A Wang

Children’s Nutrition Research Center Staff Publications

Starting at middle age, adults often suffer from visceral adiposity and associated adverse metabolic disorders. Lineage tracing in mice revealed that adipose progenitor cells (APCs) in visceral fat undergo extensive adipogenesis during middle age. Thus, despite the low turnover rate of adipocytes in young adults, adipogenesis is unlocked during middle age. Transplantations quantitatively showed that APCs in middle-aged mice exhibited high adipogenic capacity cell-autonomously. Single-cell RNA sequencing identified a distinct APC population, the committed preadipocyte, age-enriched (CP-A), emerging at this age. CP-As demonstrated elevated proliferation and adipogenesis activity. Pharmacological and genetic manipulations indicated that leukemia inhibitory factor receptor signaling was …

Effects Of Soluble Electron Shuttles On Microbial Iron Reduction And Methanogenesis, Bhim Sen Thapa, Theodore M. Flynn, Zena D. Jensvold, Kenneth M. Kemner, Margaret F. Sladek, Edward J. O'Loughlin, Christopher W. Marshall

Biological Sciences Faculty Research and Publications

In many aquatic and terrestrial ecosystems, iron (Fe) reduction by microorganisms is a key part of biogeochemical cycling and energy flux. The presence of redox-active electron shuttles in the environment potentially enables a phylogenetically diverse group of microbes to use insoluble iron as a terminal electron acceptor. We investigated the impact that different electron shuttles had on respiration, microbial physiology, and microbial ecology. We tested eight different electron shuttles, seven quinones and riboflavin, with redox potentials between 0.217 and −0.340 V. Fe(III) reduction coupled with acetate oxidation was observed with all shuttles. Once Fe(III) reduction began to plateau, a rapid …

The Dynamic Immune Behavior Of Primary And Metastatic Ovarian Carcinoma, Elaine Stur, Fuduan Peng, Pang-Ning Teng, Emine Bayraktar, Min Hu, Sara Corvigno, David J Brown, Sanghoon Lee, Kathleen N Moore, Nicholas W Bateman, Kathleen M Darcy, George L Maxwell, Thomas P Conrads, Nidhi Sahni, Ignacio Vázquez-García, Sohrab P Shah, Joseph Celestino, Nicole D Fleming, Nicholas E Navin, Linghua Wang, Anil K Sood

Faculty, Staff and Student Publications

Patients with high-grade serous ovarian carcinoma (HGSC) are usually diagnosed with advanced-stage disease, and the tumors often have immunosuppressive characteristics. Together, these factors are important for disease progression, drug resistance, and mortality. In this study, we used a combination of single-cell sequencing and spatial transcriptomics to identify the molecular mechanisms that lead to immunosuppression in HGSC. Primary tumors consistently showed a more active immune microenvironment than did omental tumors. In addition, we found that untreated primary tumors were mostly populated by dysfunctional CD4 and CD8 T cells in later stages of differentiation; this, in turn, was correlated with expression changes …

Embryonic Stem Cell Research, Huda Abu-Suwa, Lisa K. Lashley, Charles J. Golden

Essays in Developmental Psychology

Stem cell research is a controversial issue that has received considerable attention over the last three decades. Stem cell research began in the early 1980’s, when stem cells were obtained from mice embryos and utilized to understand the functioning of stem cells (Grivennikov, 2008). In 1998, researchers discovered techniques for obtaining embryonic stem cells from human and growing them in laboratories. Since them, substantial research has been conducted on stem cells and their potential uses, which includes increasing our understanding of diseases and how they occur, treating various diseases, and testing new medications for safety and efficacy (Taylor, 2005).

Acute Brcaness Induction And Ar Pathway Blockage Through Cdk12/7/9 Degradation Enhances Parp Inhibitor Sensitivity In Prostate Cancer, Fu Gui, Baishan Jiang, Jie Jiang, Zhixiang He, Takuya Tsujino, Tomoaki Takai, Seiji Arai, Celine Pana, Jens Köllermann, Gary Andrew Bradshaw, Robyn Eisert, Marian Kalocsay, Anne Fassl, Steven P Balk, Adam S Kibel, Li Jia

Faculty, Staff and Student Publications

Current treatments for advanced prostate cancer (PCa) primarily target the androgen receptor (AR) pathway. However, the emergence of castration-resistant prostate cancer (CRPC) and resistance to AR pathway inhibitors (APPIs) remains ongoing challenges. Here, we present BSJ-5-63, a proteolysis-targeting chimera (PROTAC) targeting cyclin-dependent kinases (CDKs) CDK12, CDK7, and CDK9, offering a multipronged approach to CRPC therapy. BSJ-5-63 degrades CDK12, diminishing BRCA1 and BRCA2 expression and inducing a sustained "BRCAness" state. This sensitizes cancer cells to PARP inhibitors (PARPis) regardless of their homologous recombination repair (HRR) status. Furthermore, CDK7 and CDK9 degradation attenuates AR signaling, enhancing its therapeutic efficacy. Preclinical studies, including …

A Pioneering Artificial Intelligence Tool To Predict Treatment Outcomes In Ovarian Cancer Via Diagnostic Laparoscopy, Xiaotian Ma, Yu-Chun Hsu, Amma Asare, Kai Zhang, Deanna Glassman, Katelyn F Handley, Katherine Foster, Khwahish Sharma, Shannon Westin, Amir Jazaeri, Nicole D Fleming, Pratip K Bhattacharya, Xiaoqian Jiang, Anil K Sood, Shayan Shams

Faculty, Staff and Student Publications

Ovarian cancer is associated with high rates of patient mortality and morbidity. Laparoscopic assessment of tumor localization can be used for treatment planning in newly diagnosed high-grade serous ovarian carcinoma (HGSOC). While spread to multiple intra-abdominal areas is correlated with worse outcomes, whether other morphological tumor differences are also associated with patient outcomes is unknown. Given the large volume of visual information in laparoscopic videos, we investigated whether deep-learning models can capture implicit features and predict treatment outcomes. We developed a novel deep-learning framework using pre-treatment laparoscopic images to assess clinical outcomes following upfront standard treatment, defined as short progression-free …

Frequent Failure Of Nutrients To Increase Plant Biomass Supports The Need For Precision Fertilization In Agriculture, Oliver H. Carroll, Eric W. Seabloom, Elizabeth T. Borer, W. Stanley Harpole, Peter Wilfahrt, Carlos A. Arnillas, Erika Hersh-Green, Et. Al.

Michigan Tech Publications

Implementing precision fertilization to maximize crop yield while minimizing economic and environmental impacts has become critical for agriculture. Variability in biomass response to fertilization within fields, among regions, and over time creates simultaneous risks of under-yielding and overfertilization. We quantify factors determining fertilization responsiveness (i.e., biomass increases with fertilization) up to 15 years in 61 unfertilized rangelands on six continents. We demonstrate widespread multi-year variability in responsiveness, with fertilization increasing average yield by 43% but failing to improve biomass 26% of the time. All sites were responsive at least once, but only four of 61 responded in all plots and …

Diapause And Anoxia-Induced Quiescence Are Unique States In Embryos Of The Annual Killifish, Austrofundulus Limnaeus, Patrick R. Clouser, Claire L. Riggs, Amie L. T. Romney, Jason E. Podrabsky

Biology Faculty Publications and Presentations

Diapause is a state of developmental and metabolic dormancy that precedes exposure to environmental stresses. Yet, diapausing embryos are typically stress-tolerant. Evidence suggests that diapausing embryos “prepare” for stress as part of a gene expression program as they enter dormancy. Here, we investigate if diapause II embryos of the annual killifish Austrofundulus limnaeus, which can survive for hundreds of days of anoxia, can mount a transcriptomic response to anoxic insult. Bulk RNAseq was used to characterize the transcriptomes of diapause II embryos exposed to normoxia, 4 h and 24 h anoxia, and 2 h and 24 h normoxic recovery …

Comparison Of Morphological Characteristics And Dna Sequences Of Platyhelminthes Within Dabbling And Diving Ducks Species, Elise A. Betcher, Karolyn M. Kolstad, Ashley L. Marks, Marguerite C. Reitano, Alexander J. Train

Research & Creative Achievement Day

Platyhelminthes, also known as flatworms, are among the most common parasites found in North American duck species. This phylum of parasites includes tapeworms and flukes. This study focused on identifying various Platyhelminthes found in Minnesota and Wisconsin wild ducks using both morphological characteristics and genetic sequencing. Both diving and dabbling species of ducks were used for this study, including green winged-teal (Anas carolinensis) and ring-necked ducks (Aythya collaris). Platyhelminthes were removed from legally harvested ducks and stained before examining and imaging under the microscope to identify key morphologic features for a presumptive identification. Subsequently, DNA was extracted …

Development Of A Qpcr For Detecting Borrelia Burgdorferi And Ixodes Tick Dna Simultaneously, Jenna Junker, Anthony J. Appicelli

Research & Creative Achievement Day

Black-legged ticks (Ixodes scapularis) and Lyme disease, caused by the bacterium Borrelia burgdorferi, are prevalent in the upper Midwest. Accurate determination of the percentage of Ixodes ticks that carry Lyme disease could help physicians and health experts develop treatment and prevention plans to curb the prevalence of disease. The purpose of this study was to see if accurate results for both Ixodes DNA and Borrelia DNA could be analyzed in the same qPCR assay for faster Lyme disease testing. Current testing in humans is achieved by running two separate diagnostic assays, an ELISA and Western Blot, both …

Female Acceptance Of Mates Is Consistent Across Different Sex Ratios And Male Proximities In Wood Frog (Rana Sylvatica) Breeding Aggregations, Alia Kabbaj, Laura Bousaid, Gabriella Lowney, Lindsey Swierk

Binghamton University Undergraduate Journal

Sexual selection plays a crucial role in shaping reproductive strategies. We investigated the influence of sex ratio and male proximity on female acceptance of mates in wood frogs (Rana sylvatica). We hypothesized that females at a more even sex ratio would exhibit higher acceptance of males due to reduced male competition when compared to females at a more male-skewed sex ratio. Using digital video analysis of mating trials (n = 39), we quantified male proximity to females and female acceptance of males at two sex ratios – 1:4 (F:M) and 1:7. Males at a 1:4 sex ratio maintained …

A Comparison Of The Bacterial Communities Of The Yamuna River (India) And Mississippi River (Usa), Jacob Gareis, Osvaldo Martinez, Silas Bergen

Research & Creative Achievement Day

This study compared the bacteria in the Yamuna River in India and the Mississippi River in the USA. Water samples were taken from 11 sites (2 in the Mississippi River and 9 in the Yamuna River). The bacteria were identified using 16S rRNA gene sequencing. Some phyla such as Proteobacteria and Bacteroidetes were seen in abundance regardless of country. However, principal-component analysis showed three distinct groupings: Mississippi/Ganga/Tons and other Yamuna River locations (7 sites), Yamuna River at Delhi (2 sites), Yamuna River below Glacier (2 sites) Additionally, the Yamuna River below Glacier had the highest bacterial diversity, with a Shannon …

The Economic Impact Of The Emerald Ash Borer In Inver Grove Heights Mn, Nicholas Ruch, Noah Anderson

Research & Creative Achievement Day

Emerald Ash Borer (EAB) is an insect that bores under the ash tree's bark. This effectively kills the tree by girdling its cambium. In this analysis of EAB, we looked at the financial effect it had on Inver Grove Heights, Minnesota. Expert Tree Service removed most of their ash trees and provided us with data to help calculate how much it cost. Brian Swoboda, the head forester for Inver Grove Heights, provided us with all of the information about their plan to reforest.

In total, for the years 2022 and 2023, the removals done by Expert Tree Service cost $83,690.68 …

Classification Of Trematode Species Of Wisconsin Ducks Using Morphological And Molecular Methods, Athena B. Lueken, Isabella T. Xiong, William J. Harvey

Research & Creative Achievement Day

Trematoda is a class of flatworms and it encompasses approximately 20,000 species. Trematoda has two subclasses, Digenea and Aspedogastria. The families observed in this study were Echinostomatidae and Strigeidae from the subclass Digenea. Trematoda can be found in most species of animals, usually ingested from their food source. The Trematodes in this study were collected from three different duck species including Aythya affinis (Lesser Scaup,) Anas acuta (Pintail,) and Aix sponsa (Wood Duck) donated by hunters between 2023 and 2024. All ducks were harvested in the state of Wisconsin and the dissections were performed at Winona State University, Winona Minnesota. …

Investigating Genetic Regulators Of Crystal Cell Development In Drosophila Melanogaster, Andrea Feria, Nyla Walbrook, Anisa Turaeva, Rebecca Spokony

Publications and Research

Crystal cells, a subset of Drosophila melanogaster hemocytes, are important for melanization, a crucial immune response. While the genetic regulation of crystal cell develop remains incompletely understood, this study investigates the functions of five candidate genes: ACXB, ACXA, Gp210, CG4390, and Cyp4s3; identidied through previous genome-wide association studies. Using the Gal4/UAS system along with RNA interference (RNAi), we exclusively knocked down each gene in third instar larvae and quantified crystal cell populations following heat shock-induced melanization. Our results imply that the knockdown of ACXB and ACXA significantly increased crystal cell counts in both sexes, suggesting these genes either act as …

Effects Of Gibberellin On Mutant Dwarf Setaria Viridis Height, Myles Petty, Breslin Clark, Caden Post, Shai Steineger, Hailey Moore, Andrew Thompson, Callie Demarest, Peyton Willeford

2025 Student Academic Showcase

Over the course of 16 weeks, three genotypes of Setaria viridis, two of which display dwarfism and one wildtype, were subjected to 100 micromole solution of gibberellic acid. This was done in order to identify how the height of dwarf mutant millets would change in reaction to gibberellic acid. A total of 216 plants were looked at. 72 of each genotype were used and split between two different treatments, one with gibberellic acid and one without.

You Are What You Eat: Bioaccumulation Of Microplastics In The Food Chain, Audrianna Torrey

2025 Student Academic Showcase

Over the last century, the use and production of plastic has surged as demand for cost-effective alternatives to traditional materials has grown. While plastic has been extremely beneficial in reducing the cost of production while expanding the market, it has also created a global pollution crisis. By 2015, an estimated 6,300 million tons of plastic waste had been generated with nearly 80% accumulating in landfills and the environment. Over the last couple decades, researchers have begun to study the effects of plastic waste and its breakdown into microplastic on aquatic environments. Although not all organisms reside in water, every living-being, …

Efficiency Versus Quality: A Reflection On Making A Children’S Book, Elizabeth Diehl

Honors Projects

When first looking at a children’s book, it appears to be a simple creative project. However, when one finds out the components that go into making one, it becomes clear that simplicity is deceiving. Research, ideation, rough drafts, and scripts are just a few of the necessary components for the finished product, and each one of these components can take multiple days or weeks to complete. This essay is a reflection on my experience of creating a picture book in one semester as I attempt to answer the questions “What if the most efficient process for creating a children's picture …

Trace Element Contamination In The Coastal Gray Wolves Of Southeast Alaska, Miranda Brohman

All HCAS Student Capstones, Theses, and Dissertations

Gray wolves (Canis lupus), northern sea otters (Enhydra lutris) and salmon (Oncorhynchus spp.) play vital roles in southeast Alaska, yet all are exposed to trace element (TE) bioaccumulation. This study examines dietary patterns and TE accumulation in four northern Lynn Canal wolf populations (Douglas Island, Juneau, Gustavus, and Pleasant Island) using stable isotope analysis (SIA) (δ¹³C and δ¹⁵N) and TE assessments. SIA revealed that wolves in Juneau and Douglas Island primarily consumed terrestrial prey, whereas wolves in Gustavus and Pleasant Island exhibited enriched isotopic signatures indicative of a marine-based diet, particularly …

A Population Study Of The Freshwater Turtles Of The Recently Expanded Lake Hydaway In Campbell County, Virginia, Joshua Sorenson

Senior Honors Theses

Freshwater turtles are an important part of the ecological systems in central Virginia. At the recently expanded Lake Hydaway, there are several turtle species for which their population characteristics were unknown, especially the prolific Eastern Painted Turtle (Chrysemys picta picta) and Red-bellied Cooter (Pseudemys rubriventris). For five weeks, the lake was surveyed for turtles using hoop nets, and the turtles were marked and measured. Population characteristics such as carapace length and sex ratio for the Eastern Painted Turtle and Red-bellied Cooter were calculated, as well as population estimates for those two species. The Red-eared Slider ( …

The Application Of Gibberellin On Dwarf Mutant Millet Plant (Setaria Viridis), Catherine Philipps, Desirae Snyder, Elizabeth Dill, Joselyn Wood, Kaneez Moughal, Katherine Kellner, Rachel Wilson

2025 Student Academic Showcase

Gibberellin is a hormone found in many plants whose purpose is to promote the growth of stems and leaves. The application of gibberellin in differing concentrations was applied to different strains of dwarf mutant millet plants and was compared to a control of nonmutant wild-type millet plants. After the initial sprouting of each seed, 4 different concentrations of gibberellin: 0g gibberellin/1L water, 0.02g/L, 0.05g/L, and 0.15g/L, were applied to the stem of each plant. A regression analysis was performed on the heights of each plant to determine the effectiveness of the varying gibberellin concentrations on the growth of millet plants. …

Dietary Preferences And Food-Seeking Behavior Of Discoid Cockroaches, Blaberus Discoidalis, Claire O'Brien, Darcci Freie, Robert Larson

2025 Student Academic Showcase

Blaberus discoidalis, commonly known as discoid cockroaches, are native to South American tropics where they consume leaf litter and decaying organic matter on forest floors. This experiment expands upon previous research concerning the diet preference of discoid cockroaches based on their loitering time when presented with different food types. Utilizing their preferred foods, peanuts, leaf litter, and banana, this experiment aims to determine how much of each food is being consumed to further support their diet preferences.

Gibberlin In Millet Physiology, Torrie Ens, Bella Reaka, Claire Albrecht, Hannah Smallwood, Will Graham, Adam Staley, Garrett Steger, Erin Strong

2025 Student Academic Showcase

Gibberellin is a hormone in plants that affects growth. Gibberellin works to begin germination in grass seeds by stimulating amylase production. It may be possible to use gibberellin to understand why different millet variants exhibit dwarf growth. We grew and treated 4 different dwarf variants with a gibberellic acid solution to identify the ability of each plant to utilize gibberellin. We compared the growth in millet grown both with and without the solution to see the effects of gibberellin on each variant. Dwarf millets may not produce their own gibberellin, or they may lack the receptors to utilize gibberellin. Our …

Analysis Of Differentially Expressed Genes (Degs) Related To Interleukin-17 Signaling For Biomarker Identification And Therapeutic Targets In Atopic Eczema, Siva Fauziah, Weri Veranita, Siti Nurbaya, Puji Sari

Indonesian Journal of Medical Chemistry and Bioinformatics

Atopic eczema, also known as atopic dermatitis, is a chronic inflammatory skin condition characterized by itchy, red, and swollen skin. It is often associated with other atopic diseases such as asthma and hay fever. Interleukin-17 (IL-17), a pro-inflammatory cytokine, plays a crucial role in various inflammatory and autoimmune conditions, including atopic eczema. This study aims to identify potential therapeutic targets for managing atopic eczema based on the analysis of differentially expressed genes (DEGs). The expression of these gene targets was subsequently validated for their potential as biomarkers. Additionally, upstream regulator protein (URP) searches for the resulting DEGs were conducted. DEG …

High-Grade Astrocytoma With Piloid Features: A Single-Institution Case Series And Literature Review, Eric A Goethe, Subhiksha Srinivasan, Swaminathan Kumar, Sujit S Prabhu, Maria A Gubbiotti, Sherise D Ferguson

Faculty, Staff and Student Publications

High-grade astrocytoma with piloid features (HGAP) is a recently described primary brain tumor and the first requiring a specific methylation pattern for diagnosis, as its histologic features are often compatible with other tumors such as glioblastoma (GBM). Characterized by molecular alterations in CDKN2A/B, NF1, BRAF, FGFR1, and ATRX, they may be located anywhere in the CNS but show a predilection for the posterior fossa. Reports are limited to retrospective case series, and the standard of care is not yet established. We performed a retrospective review of electronic medical records of all patients with HGAP at our institution. Records were queried …

Idh Status Dictates Ohsv Mediated Metabolic Reprogramming Affecting Anti-Tumor Immunity, Upasana Sahu, Matthew P Mullarkey, Sara A Murphy, Joshua C Anderson, Vasanta Putluri, Abu Hena Mostafa Kamal, Jun Hyoung Park, Tae Jin Lee, Alexander L Ling, Benny A Kaipparettu, Ashok Sharma, Nagireddy Putluri, Pamela L Wenzel, Christopher D Willey, E Antonio Chiocca, James M Markert, Balveen Kaur

Faculty, Staff and Student Publications

Identification of isocitrate dehydrogenase (IDH) mutations has uncovered the crucial role of metabolism in gliomagenesis. Oncolytic herpes virus (oHSV) initiates direct tumor debulking by tumor lysis and activates anti-tumor immunity, however, little is known about the role of glioma metabolism in determining oHSV efficacy. Here we identify that oHSV rewires central carbon metabolism increasing glucose utilization towards oxidative phosphorylation and shuttling glutamine towards reductive carboxylation in IDH wildtype glioma. The switch in metabolism results in increased lipid synthesis and cellular ROS. PKC induces ACSL4 in oHSV treated cells leading to lipid peroxidation and ferroptosis. Ferroptosis is critical to launch an …

Meta-Transcriptomes Of The Microbial Communities Of The Different Developmental Life Stages Of Anastrepha Ludens (Diptera: Tephritidae), Erin L. Schuenzel

School of Integrative Biological & Chemical Sciences Faculty Publications

The Mexican fruit fly, Anastrepha ludens, is a polyphagous pest that is a constant threat to US agriculture because of its proximity to citrus production in border region of the United States and Mexico. Sterile Insect Technique has been used to manage and eradicate of the Mexican Fruit Fly. SIT, however, requires millions of sterile male flies to be released each week from mass-rearing facilities. The mass-reared colonies must deal with microbial infections that reduce survivorship, fecundity and male-male competition. Meta-transcriptomics joins culturing and next generation DNA sequencing techniques to understand these changing microbial communities. This study uses advances …

2025 Acssc Program, Acssc Planning Committee

Annual Celebration for Student Scholarship and Creativity

No abstract provided.

Args & Chronic Disease: How Antibiotic Resistance Genes Impact The Progression Of Type Ii Diabetes, Paige Imperato

Thinking Matters Symposium

Antibiotics are typically offered to patients with no warning of long-term consequences, though increasing evidence suggests this may not be entirely true. When antibiotics enter the body, they kill both good and bad bacterial populations in the gut - collectively known as the human microbiome. Repeated use of these drugs encourages mutations in this bacterial population [2]. This leads to the creation of antibiotic-resistant genes (ARGs) in antibiotic resistant bacteria (ARB). The causal relationship between antibiotic use and bacterial resistance is well established. However, recent research suggests a newly discovered relationship between the presence of specific ARGs in the microbiome …

A Redescription Of Leiurus Libycus (Birula, 1908), Stat. N. (Scorpiones: Buthidae), Ersen A. Yağmur, František Kovařík, Victor Fet

Euscorpius

Buthus quinquestriatus libycus was described by Birula (1908) from the Libyan Desert, based on a subadult male specimen. This taxon was later synonymized with Leiurus quinquestriatus (Ehrenberg, 1828) by Vachon (1949). In this study, we re-evaluate this taxon and recognize it as Leiurus libycus (Birula, 1908), stat. n., providing a redescription based on the holotype. The type locality of this species is clarified as western Egypt (between Alexandria and Fayum). We synonymize Leiurus aegyptiacus Lourenço & El-Hennawy, 2021 with L. libycus, stat. n., syn. n.