Temporal Assessment Of Fish Communities In A South Central Plains Ecoregion Watershed, Tara L. Schnelting

ATU Theses and Dissertations 2021 - Present

Among South Central Plains ecoregion fish communities, there is limited knowledge of the importance of headwaters yet this information is needed to understand their role in fish communities. I addressed this gap by assessing seasonal changes in fish communities in headwater streams of the Moro Creek watershed, Arkansas. I conducted two studies pertaining to temporal fish community assessments: (i) utilize multi-season, community occupancy models to estimate μ (species probability), Ω (community occupancy mean), and p (detection probability) and (ii) utilize beta diversity (β) analyses to address the diversity across different scales in the headwaters of the Moro Creek …

Coxiella Burnetii Manipulation Of Membrane Contact Sites In The Intracellular Niche, Margaret F. Sladek

Theses & Dissertations

Coxiella burnetii is a highly infectious and environmentally persistent bacterium that causes the disease Q Fever. Its intracellular home, the Coxiella-Containing Vacuole (CVV), is formed from fusion of host-derived vesicles and is rich in sterols - yet cholesterol accumulation in the CCV is bacteriolytic. Our lab’s goal is to understand how Coxiella manages intracellular cholesterol levels and survives within the host. One way Coxiella may modulate CCV cholesterol is through membrane contact sites (MCS), areas where molecular complexes bridge two organelles and exchange lipids. This thesis explores how Coxiella uses both a mammalian lipid transfer protein and bacterially-encoded effector …

Schistosoma Haematobium Infection In Ethiopian Children: Evaluating Praziquantel Efficacy, Nutritional Effects, And Diagnosis Using Flukecatcher, Louis Fok

Theses & Dissertations

Urogenital schistosomiasis continues to impact the wellbeing of hundreds of millions of people worldwide and remains a significant public health challenge across Ethiopia. Despite existing knowledge on the disease, there remain significant unanswered questions with regard to its treatment and diagnosis. This dissertation evaluated the efficacy of praziquantel at treating urogenital schistosomiasis, improving nutritional parameters among children and the performance of FlukeCatcher at detecting and determining the intensity of infection. In effecting this, a total of 977 children aged 5 through 15 from the Afar and Gambella regions of Ethiopia were screened for S. haematobium infection using urine filtration microscopy …

Cholestasis And Fluid Flow Are Biomechanic Regulators Of Cholangiocarcinoma Progression, Andrew Oleksijew

Theses & Dissertations

Cholestasis, altered or absent bile flow, is associated with poor survival in patients with cholangiocarcinoma, an aggressive cancer of the biliary epithelium. Changes in bile flow often result in stricture or obstruction. Experimental cholestasis promoted tumor growth and progression. However, studies are needed that directly assess the mechanical interaction between bile flow, or bile shear stress, and cholangiocarcinoma signaling. We hypothesized that cholestasis and absent bile shear stress activated cancer signaling and increased aggressive cellular behaviors such as proliferation and migration. Fluid shear stress approximating bile flow was applied by orbital culture plate method and compared to identical static culture …

Validating The Ability Of Iag933 To Block The Interaction Of Yap1 And Tead In Ovarian Cancer Cells, Alex Sage

Theses & Dissertations

Ovarian cancer is the most common and deadliest gynecologic malignancy. Approximately 40% of ovarian cancer patients exhibit chemoresistance after receiving initial treatment in the form of neoadjuvant chemotherapy or interval debulking surgery, of which YAP1 (Yes-associated protein 1) has been implicated in enhancing the resistance of ovarian cancer to Cisplatin and Taxol.^1,2. This highlights the need to develop new therapeutic strategies that target the Hippo pathway to overcome resistance to traditional therapies.

YAP1 is a protein that acts as a transcription coregulator, promoting the transcription of genes involved in cellular proliferation and suppressing apoptotic genes. It is a …

Apolipoprotein E4, Tauopathy, And Brain Injury: Molecular Insights Into Alzheimer's Disease Pathology, Nashanthea J. Roland

Theses & Dissertations

Alzheimer’s disease (AD) is the world’s leading cause of dementia, characterized by progressive atrophy of the hippocampal and cortical brain regions, synapse damage and loss, and a buildup of senile plaques and neurofibrillary tangles composed of β-amyloid peptides and hyperphosphorylated tau, respectively. While age is the most significant risk factor for developing AD, apolipoprotein E4 (APOE4) is the strongest genetic risk factor for AD. Although it is known that APOE4 influences tau pathology, the molecular mechanisms by which APOE4 drives AD pathogenesis and progression remain unclear. In addition to intrinsic contributions to AD, there are many extrinsic factors that can …

Drivers And Mechanisms Of Golgi Fragmentation As A Modulator Of Disease, Amanda Macke

Theses & Dissertations

The Golgi apparatus plays a pivotal role in post-translational modification, trafficking, and secretion of proteins, ensuring proper cellular function. As a result, its fragmentation has been implicated in multiple pathological conditions, including alcohol-induced liver injury and prostate cancer (PCa). Despite its known impact on cellular homeostasis, the molecular mechanisms governing Golgi fragmentation remain to be clarified. Additionally, we have only just begun to understand the vast consequences of Golgi disorganization on disease progression.

This dissertation investigates the drivers and mechanisms of Golgi fragmentation, with a focus on its role as a modulator of disease. Using both in vitro and in …

Intrinsic And Extrinsic Drivers Of Bioaerosol Decay: Investigating Pathogen Stability Of Bacteriophage Ms2, Sin Nombre Virus, And Sars-Cov-2 In Simulated Environments, Elizabeth A. Klug

Theses & Dissertations

The threat posed by a virus as a bioaerosol depends largely on its stability under various environmental conditions (relative humidity (RH), sunlight, ozone, etc.). This stability is typically studied through aerosol decay—how the virus' viability changes over time when exposed environmental conditions. The Goldberg rotating drum, a widely used tool for studying bioaerosols, has been the basis of such research (Goldberg et al., 1958). However, it has significant limitations, including an inability to measure initial pathogen infectivity loss and the use of small sample volumes. Additionally, it does not answer the question of what intrinsic factors (lipids, proteins, nucleic acids) …

Investigating The Effects Of Transcription Factor Binding And Genetic Variants In The Striatum Of Post-Mortem Cohorts With Opioid Use Disorder, Rajashree Chakraborty

Theses & Dissertations

The opioid crisis has emerged as one of the most pressing public health challenges of our time, with Opioid Use Disorder (OUD) affecting millions of lives across the globe. Studying OUD is not merely an academic pursuit but a critical necessity in addressing this multifaceted epidemic. The urgency of this research is underscored by the staggering prevalence of OUD, with an estimated 3.7% of U.S. adults requiring treatment in 2022 alone. Despite the availability of effective medications for OUD, a significant treatment gap persists, with only a quarter of those in need receiving these life-saving interventions. The far-reaching consequences of …

Claiming More To Cope: Does Ostracism Increase Overclaiming?, Katy Q. Chen

Honors Theses

In a pre-registered online study, we investigated the relationship between ostracism and overclaiming. Ostracism is the act of deliberately or unintentionally excluding and ignoring others. Overclaiming refers to the behavior of exaggerating one’s knowledge or familiarity with specific topics, often as a way of attempting to present oneself as more knowledgeable or competent than one actually is. Drawing from the temporal need-threat model, we hypothesized that ostracized individuals, as a compensatory response, would likely overclaim more compared to included individuals. This online study simulated a work experience where participants were asked to answer questions about their work-related experiences and opinions. …

The Impact Of The Mediterranean Diet On Depression: A Narrative Review, Emma Kate Schaaf

Honors Theses

Background. The Western diet has extensive negative ramifications on physical health and emotional well-being. The Mediterranean diet, widely regarded as one of the healthiest eating patterns in the world, may have the ability to improve psychological conditions. This study aims to examine existing research from randomized control trials (RCTs) on the association between depression prevalence and the Mediterranean diet.

Methods. The following review was conducted using PRISMA guidelines and the Google Scholar and PsycInfo databases. The criteria for inclusion were that studies: 1) were randomized control trials (RCTs) examining the Mediterranean diet; 2) included samples of adults (i.e., > 18 years …

The Impact Of Antimicrobial Peptide Depletion And Fungal Infection On Thermal Preference In Green Frogs, Rana Clamitans, Kim M. Salvador

Honors Theses

Climate change and the disease chytridiomycosis, caused by the fungal pathogen Batrachochytrium dendrobatidis (Bd), are the two main drivers of amphibian decline worldwide, but it is difficult to predict the outcomes of these interacting stressors. Amphibians are ectotherms meaning they are dependent on environmental temperature for the maintenance of physiological processes, and they rely on behavioral thermoregulation to maintain body temperatures within their thermal limits. As the climate becomes more variable, amphibians are also potentially more susceptible to diseases such as Bd, as their immune defenses, such as antimicrobial peptide (AMP) defenses of the skin, take longer to …

Impacts Of Polyploidization On Endocrine Regulation And Vocalization In The Gray Treefrog Complex, Avery Thigpen

Honors Theses

Comparative genomics data suggest that vertebrates are descendants of ancestral polyploids and that historical polyploidization events fueled evolutionary innovations. For example, polyploidization can modify gene expression patterns and/or increase gene mutation rates to alter the phenotype. While considerable emphasis has been placed on the evolutionary impact of ancient polyploidization events, less is known regarding phenotypic modifications in extant polyploids. I examined circulating levels of adrenal glucocorticoids and gonadal steroids in the diploid-tetraploid gray treefrog complex to understand how polyploidization has impacted hormonal regulation. Furthermore, I examined the relationships between levels of these hormones and acoustic signals to examine if and …

The Endocannabinoid System's Influence And Modulation Of Nociception, Doreen M. Flannery

Honors Thesis

The anatomy and physiology of pain is a well-defined pathway where noxious stimuli is processed by different systems in the brain. The central research question of this review is how can we better understand the neurobiological effects pain has, and assess if there is therapeutic potential in using endocannabinoids to modulate these effects. Endocannabinoids are signaling molecules that play a key role at both the cellular and molecular level in modulating both acute and chronic pain by influencing different processes within each level that affect how the body responds to both internal and external stimuli. The endocannabinoid system is a …

Cholesterol Mass Fingerprinting To Probe Cholesterol-Protein Interactions, Warren Lowther

Electronic Theses and Dissertations

Cryomicroscopy, crystallography and NMR have been considered the “gold standard” of structural biophysics for some time. Recent successes in using these techniques has provided us with a plethora of insight into the architecture of different macromolecules like channels, transporters, and other molecular machines. However, information is often limited to a single allosteric state with the macromolecule in a non-native environment. Additionally, these traditional methods tend to be expensive and labor-intensive, requiring samples that are concentrated and highly purified. Chemical crosslinking coupled with mass spectrometry (CX-MS) has emerged as the ideal accessory for the aforementioned structural tools. In order to address …

Neurodegenerative Disease Mechanisms: Mitochondrial Impairment, Tau Aggregation, And Neuroinflammation As Central Drivers Of Disease Progression, Luis D. Estrella

Theses & Dissertations

The study of the intricate molecular mechanisms behind the pathology of neurodegenerative diseases has become a primary research area in the past decades. The major branches of these studies involve both disease progression and disease pathogenesis, in which neuroscientists have studied, and continue to study, the role that mitochondrial dysfunction, aberrant protein aggregation (like Tau), and immune cell reactions have on these. However, more recent studies have shined light to the fact that these three pathological features are not independent from one another and that their synchronous contributions may be the ultimate driver of neurodegeneration. This dissertation encompasses four different …

The Role Of Presynaptic Proteins In Alcohol Sensitivity And Tolerance, Madhumitha Gundluru

Honors Theses

Mammalian Unc13 (Munc13) and Drosophila Unc13 (Dunc13) are presynaptic active zone proteins essential for synaptic vesicle docking and priming in a neuron. Alcohol binds to the C1 domain of Munc13, which inhibits DAG binding. The reduction in DAG binding by Munc13 decreases the probability of vesicle fusion with the membrane, decreasing neurotransmitter release. Heterozygotes for a Dunc13 loss-of-function allele are behaviorally resistant to alcohol, physiologically resistant to alcohol, molecularly resistant to alcohol, and self-administer significantly more alcohol than control flies. Therefore, genetically reducing Dunc13 mimics a state of alcohol tolerance in the fly. The two main Dunc13 isoforms, Dunc13A and …

Menthalactone From Mentha Piperita L., A Monocot-Selective Bioherbicide, Adam Soltani

Honors Theses

The challenge of managing invasive weed species continues to affect the agricultural industry, presenting ecological, economic, and agronomic hurdles that lead to over 100 billion USD in annual crop losses globally. One such concern is the management of Agrostis stolonifera L., commonly known as creeping bentgrass, particularly due to its ability to form hybrids. This scenario underscores the urgent need for innovative, effective, and environmentally sustainable herbicides, steering the focus toward natural substances as potential candidates. We report here a promising natural lactone, commonly known as menthalactone, which is derived from Mentha piperita L. Its phytotoxic activity was assessed against …

Fine-Scale Spatial Patterns In Microbial Activity And Community Composition In Shoreline Marine Sediments, Elizabeth Basha

Honors Theses

Coastal environments of the Gulf of Mexico host diverse and dynamic microbial communities essential for biogeochemical cycling, yet the fine-scale environmental drivers of microbial function and community structure in these systems remain underexplored. This study investigated the relationships between sand moisture content, microbial extracellular enzyme activity, and bacterial community composition along beach transects on Biloxi East Beach, Mississippi. Sand was sampled at five points along four transects spanning a gradient from submerged intertidal zones to dry upper beach sands. Extracellular enzyme activities linked to carbon, nitrogen, and phosphorus cycling (β-glucosidase, N-acetylglucosaminidase, phosphatase, phenol oxidase, and peroxidase) were measured, and bacterial …

Academic Burnout In Human Anatomy & Physiology I Students, Shivani Shukla

Honors Theses

Human Anatomy and Physiology I is the study of the function and structure of the human body. It is a challenging class that many students have to take for their undergraduate degree. Due to the vast information that is given in this complex class, students can struggle with understanding the material and knowing how to study thus leading to burnout in the course. Understanding why students undergo burnout in their courses could uncover ways that professors and institutions could help the students to prevent burnout. The goal of this study was to determine the level of burnout in Human Anatomy …

Assisted Reproductive Technologies For Southern Cricket Frogs (Acris Gryllus), Audrey Grace Koltz

Honors Theses

Amphibians are facing the most drastic biodiversity declines of all vertebrates, with an estimated 41% of amphibians threatened with extinction. Captive breeding programs employ a variety of assisted reproductive technologies including environmental manipulation, hormonal therapies, artificial fertilization, and gamete preservation to augment vulnerable populations and optimize genetic diversity. Amphibians require a species-specific combination of environmental, social, and physiological cues to reproduce, which makes universal and successful captive breeding programs especially elusive. Here, I investigate assisted reproductive technologies for a small frog species, the southern cricket frog (Acris gryllus). Establishing successful methods for southern cricket frogs may yield promising results for …

Allogeneic Chimeric Antigen Receptor T-Cell Products Cemacabtagene Ansegedleucel/Allo-501 In Relapsed/Refractory Large B-Cell Lymphoma: Phase I Experience From The Alpha2/Alpha Clinical Studies, Frederick L Locke, Javier L Munoz, Michael T Tees, Lazaros J Lekakis, Sven De Vos, Rajneesh Nath, Don A Stevens, Shahbaz A Malik, Geoffrey P Shouse, Mehdi Hamadani, Olalekan O Oluwole, Miguel-Angel Perales, David B Miklos, Paul W Fisher, Amy Feng, Lynn Navale, John B Le Gall, Sattva S Neelapu

Faculty, Staff and Student Publications

Purpose: Off-the-shelf, allogeneic CD19 chimeric antigen receptor (CAR) T-cell products may improve access to treatment versus autologous ones. We report the phase I experience of the allogeneic CD19 CAR T-cell product cemacabtagene ansegedleucel (cema-cel) and its predecessor, ALLO-501, in CD19 CAR T-naïve patients with relapsed/refractory large B-cell lymphoma (R/R LBCL).

Methods: In the ALPHA2/ALPHA studies, the safety and efficacy of allogeneic CD19 CAR T cells were evaluated in CD19 CAR T treatment-naïve patients with R/R LBCL. Patients received healthy donor-derived, human leukocyte antigen-unmatched cema-cel/ALLO-501 following a 3-day lymphodepletion regimen of fludarabine (30 mg/m2 once daily), cyclophosphamide (300 or 500 mg/m2 …

Moths Of The Douglas Lake Region (Emmet And Cheboygan Counties), Michigan: Vii. Tortricidae (Lepidoptera), Brian G. Scholtens, Michael Sabourin

The Great Lakes Entomologist

Two hundred thiry-four species of Tortricidae are documented from Emmet and Cheboygan counties in Michigan, including 32 state records. This is the second major taxon of microlepidoptera inventoried from the area, and provides the first significant listing of a regional tortricid fauna from the state. Several additions are expected for this list as additional habitats and seasons are sampled more effectively.

The Caddisflies (Trichoptera) Of An Undisturbed Lower Michigan Habitat, David C. Houghton

The Great Lakes Entomologist

The caddisfly assemblages of small streams, medium floodplain and forested rivers, and lakes within the Black River Ranch, a relatively undisturbed habitat in northern Lower Michigan, were sampled during 2014–2015 using ultraviolet light traps to establish a reference assemblage for the caddisflies of the region. A total of 117 species were collected including three—Hydroptila amoena Ross, H. novicola Blickle and Morse, and H. quinola Ross—not previously reported from Michigan. All species and their habitat affinities are reported herein. Individual sampling sites all had high levels of species richness compared to similar habitat types throughout Michigan, Minnesota, and Wisconsin, and included …

Consumption Of Insect Pests By The Evening Bat (Nycticeius Humeralis) In Southeastern Michigan, Olivia M. Münzer, Brian Schaetz, Allen Kurta

The Great Lakes Entomologist

Diet of a colony of evening bats (Nycticeius humeralis Rafinesque) in Lenawee County, Michigan, was studied during 2006–2007, using standard fecal analysis. Of the 594 fecal pellets examined, the spotted cucumber beetle (Diabrotica undecimpunctata howardi Barber: Coleoptera), green stink bug (Chinavia hilaris Say: Hemiptera), and emerald ash borer (Agrilus planipennis Fairmaire: Coleoptera) were identified and occurred at a frequency of 15, 12, and 0.8%; respectively, over the two years combined. In some weeks, up to 64% of the pellets analyzed contained fragments of spotted cucumber beetles, and up to 43% contained green stink bugs—two of the most injurious pests to …

Spatiotemporal Patterns Of Population Decline In Oarisma Poweshiek (Hesperiidae) In Michigan And Minnesota Between 1990 And 2013, Elise C. Rosengren, David A. Andow

The Great Lakes Entomologist

The recent contraction of the geographic range of Oarisma poweshiek (Poweshiek skipperling) (Lepidoptera: Hesperiidae) resulted in its listing as an endangered species in 2014 (USFWS 2014). However, changes in population density within sites associated with this contraction are still not understood. This range-wide contraction of Poweshiek skipperling appears to indicate a regional cause. Using population data from multiple sites in Michigan and Minnesota, we investigated the temporal and spatial patterns of population decline to determine if regional or site-specific factors likely contributed to the decline. We hypothesized that if regional factors caused the density declines, then the declines would be …

Associations Among Serropalpus Substriatus (Coleoptera: Melandryidae) And Sirex (Hymenoptera: Siricidae) Communities, Tonya D. Bittner, Ann E. Hajek, James K. Liebherr

The Great Lakes Entomologist

Serropalpus substriatus Haldeman (Coleoptera: Melandryidae) develops within the same trees as Sirex noctilio F. and Sirex cyaneus F. (Hymenoptera: Siricidae). This species is now reported emerging from red pine (Pinus resinosa Sol. ex Aiton) and balsam fir (Abies balsamea (L.) Mill.) (Pinaceae) in eastern North America. Numbers of Se. substriatus emerging from pines were always much lower than numbers of Sirex in trees, and 18% of Se. substriatus required 2 years before emergence. During first years of emergence, Se. substriatus was found in only 12 of 41 sampled red pines infested by Si. noctilio. Trees from which Se. substriatus emerged …

The Biogeography Of A Disjunct Plant-Insect Relationship: Thimbleberry And Diastrophis Kincaidii (Hymenoptera: Cynipidae) In The Great Lakes Region, Michael C. Rotter, Mac Strand

The Great Lakes Entomologist

The clonal shrub thimbleberry (Rubus parviflorus) ranges throughout western North America and is disjunct to the northern Great Lakes region. Diastrophis kincaidii (Hymenoptera: Cynipidae) is an obligate gall wasp of thimbleberry that has long been known from western North America but has only been reported from the Great Lakes region since the early 1990s. It has been suggested that Diastrophis kincaidii was only recently introduced into the Great Lakes region sometime in the 1980s. We conducted a survey to determine the distribution of D. kincaidii and its parasitoids within the thimbleberry range in the Great Lakes region. We found that …

A New Species Of Erioptera Meigen Crane Fly (Diptera: Limoniidae) From Michigan With Notes On Closely Related Species, Stephen W. Taber

The Great Lakes Entomologist

A new Nearctic species of Erioptera Meigen crane fly was discovered in Michigan, USA. It belongs to the green chlorophylla species group of the nominate subgenus. Erioptera michigana Taber sp. n. is most closely related to Erioptera subfurcifer Alexander but their reproductive structures are different. Their close relative Erioptera georgei Alexander is illustrated for the first time.

Understanding Temporal Changes In Leaf Traits Of Native Smilax Rotundifolia And Invasive Lonicera Japonica Using Long-Term Herbarium Records From Kentucky, Claudia Ebbecke, Claudia Ebbecke

Honors College Theses

Herbarium specimens are the most valuable, verifiable, and sustainable records to study the impacts of climate change on natural ecosystems. Temporal herbarium data can provide critical information on how species respond to changes in habitat conditions by tracking changes in species’ morphological and phenological traits. This study aims to use Japanese honeysuckle (Lonicera japonica) and common greenbrier (Smilax rotundifolia) to assess the impact of climate change on invasive and native plants. Leaf traits, such as leaf size and stomatal density are good indicators of plant’s adaptation to the changing environment. By examining changes in leaf traits …