Digital Commons Network^™

Insect Farming Innovations: A Pathway To Self-Reliance In Agrifood Systems In Africa, Zewdu Abro, Menale Kassie, Dennis Beesigamukama, Shaphan Yong Chia, Ermias Engida Legesse, Subramanian Sevgan, Julius Ecuru, Chrysantus M. Tanga

All Peer-Reviewed Publications

Insect farming as an innovative approach to transforming agrifood systems

Insect farming has emerged as a transformative and sustainable approach to addressing the interconnected challenges of food insecurity, environmental degradation, and economic vulnerability in Africa’s agrifood systems. By converting organic waste into high-value products (protein-rich feed, frass fertilizers, and bioactive compounds), insect farming offers an efficient circular economy solution that bridges nutrition, livelihoods, and ecological resilience (Lalander et al. 2025; Mei et al. 2024; Barragán-Fonseca et al. 2022; Xia et al. 2021; Van Huis 2020; Barragán-Fonseca et al. 2017; Van Huis and Oonincx 2017; Yi et al. 2014). Almost all …

Ecological Intensification Of Maize-Kale Systems In Kenya By Biofertilization And Push-Pull Management Shifts Nematode Communities, Agnes Kiriga, Johnnie Van Den Berg, Frank Chidawanyika, Solveig Haukeland, Bianca Greyvenstein, Dennis Beesigamukama, Chrysantus M. Tanga, Sevgan Subramanian, Daniel M. Mutyambai

All Peer-Reviewed Publications

Plant-parasitic nematodes (PPNs) pose a persistent threat to vegetable production in Kenya, contributing to reduced yields and soil degradation. The management of PPNs in vegetables in Kenya has largely remained unaddressed. The integration of ecological intensification strategies such as the vegetable integrated push-pull (VIPP) system and the use of black soldier fly frass fertilizer (BSFF) is gaining traction in sustainable agriculture. The aim of this study was to determine the effects of integrating VIPP and BSFF on nematode abundance and diversity in maize-kale cropping systems. Field trials were conducted in two different agroecological zones in Kenya. Field treatments involved plots …

Brief Therapy Dog Interactions May Increase Self-Efficacy And Lower Perceived Stress And Cortisol In High School Students: A Preliminary Study, Dawn Melzer, Deirdre Yeater, Madison Derubis, Christiana Rose Kohlroser, Barbara J. Pierce

Psychology Faculty Publications

The present study examined changes in high schoolers’ perceived stress, general self-efficacy and cortisol levels after a brief interaction with a certified therapy dog. High school students completed self-report measures of perceived stress and general self-efficacy and provided salivary cortisol samples both before and after a 10-min interaction with a certified therapy dog. Following the interaction, students reported reductions in perceived stress scores, exhibited lower salivary cortisol concentrations and demonstrated increases in self-efficacy scores compared to before the interaction. Although higher self-efficacy scores were observed, general self-efficacy is generally regarded as a stable construct; therefore, these findings may reflect a …

Integrative Multi-Omics Profiling Identifies Infiltrative Hepatocellular Carcinoma As An Immunotherapy-Resistant Subtype With Distinct Molecular Features, Won Suk Lee, Seonjeong Woo, Sung Hwan Lee, Gae Hoon Jo, Ilhwan Kim, Hyeyeong Kim, Chansik An, Sanghoon Jung, Gwangil Kim, Haeyoun Kang, Beodeul Kang, Jung Sun Kim, Ho Yeong Lim, Incheon Kang, Hannah Yang, So Jung Kong, Dahyeon Son, Dong Jun Shin, Woo Young Kwon, Da-Yeon Lee, Ju-Seog Lee, Junho Park, Youngsoo Kim, Sohyun Hwang, Chan Kim, Hong Jae Chon

Faculty, Staff and Student Publications

Background/aims: Hepatocellular carcinoma (HCC) exhibits substantial morphological and biological heterogeneity. Clinical and molecular relevance of the infiltrative subtype remains poorly defined in the context of cancer immunotherapy. We aimed to evaluate the prognostic impact and molecular features of infiltrative HCC in patients treated with first-line atezolizumab plus bevacizumab (Ate/Bev).

Methods: We included 307 patients with advanced HCC treated with Ate/Bev and classified them into four gross morphological types based on imaging. Multi-omics profiling was conducted on tumor samples. Type IV infiltrative signature was derived and externally validated using five independent HCC cohorts, including IMbrave150.

Results: Infiltrative morphology, encompassing pure and …

Engineering Mutation Clones In Mammalian Cells With Crispr/Cas9, Zijun Huo, Jian Tu, Rachel Shoemaker, Dung-Fang Lee, Ruiying Zhao

Faculty, Staff and Student Publications

CRISPR, Clustered Regularly Interspaced Short Palindromic Repeat, as a powerful genome engineering system, has been widely accepted and employed in gene editing of a vast range of cell types. Compared to zinc finger nucleases (ZFNs) or transcription activator-like effector nucleases (TALENs), CRISPR shows a less complicated process and higher efficiency. With the development of different CRISPR systems, it can be used not only to knock out a gene but also to make precise modifications, activate or repress target genes with epigenetic modifications, and even for genome wide screening. Here we will describe the procedure of generating a stable cell line …

Poly(A) Probe Hcr Rna-Fish Specifically Marks Pyriform Nurse Cells In The Brown Anole Lizard Ovary, Zoe B Griffin, Bonnie K Kircher, Richard R Behringer

Faculty, Staff and Student Publications

Hybridization chain reaction RNA-fluorescent in situ hybridization (HCR RNA-FISH) is a powerful and increasingly used method for visualizing gene expression in cells and tissues. A probe set against polyadenylated RNA (poly(A)) is often used as a positive control for RNA integrity and staining quality. While optimizing this technique in the ovary of the brown anole lizard (Anolis sagrei), we found that the poly(A) probe produced a strikingly specific and intense signal in pyriform cells, a specialized lizard-specific nurse cell type. This staining pattern was found in both whole-mount samples and paraffin sections, suggesting that poly(A) signal intensity can serve as …

Integrating Multi-Method Monitoring To Characterize Seasonal Habitat Use Of Green Sturgeon (Acipenser Medirostris) In Humboldt County, California, Olivia S. Boeberitz

Cal Poly Humboldt theses and projects

Green sturgeon (Acipenser medirostris) are a long-lived, anadromous, iteroparous species that spawns in large West Coast North American rivers but ranges widely in the coastal ocean. For coastal California Native American Tribes, green sturgeon are a vital part of their cultural heritage and traditions, and central to their food security. Two genetically distinct population segments (DPSs) of green sturgeon are recognized in the United States, Canada, and Mexico; the southern DPS is listed as “threatened” under the Endangered Species Act and the northern DPS is considered of “conservation concern.” Although green sturgeon are known to use large estuaries …

Factors Influencing Tree Vigor And Structural Failure Along Powerline Corridors In Northern California: A Multi-Species Analysis, David Justin Dorval

Cal Poly Humboldt theses and projects

Tree failures (e.g., stem breakage, uprooting, or branch drop) can pose significant risks to public safety, particularly along powerline corridors for the risk of power outages and wildfire ignitions. In California, tree-related powerline failures have led to severe ecological and human costs in recent years. Despite extensive research on tree failure in urban environments and post-disturbance contexts, comparatively little empirical work has examined where powerlines intersect largely unmanaged stands. This study evaluates tree failure trends across multiple forest types in Northern California by examining how tree-level characteristics influence recent growth and failure occurrence in regionally dominant tree species. Specifically, the …

Baker Cypress (Hesperocyparis Bakeri) In A Changing Fire Landscape: Characterizing Fire Refugia And Guiding Post-Fire Reforestation, Olivia Lorraine Moskowitz

Cal Poly Humboldt theses and projects

Increasing wildfire frequency threatens serotinous, fire-obligate species that depend on sufficient fire-free intervals to develop seed banks for reproduction. These species require fire intense enough to trigger seed release and create favorable substrate and light levels for regeneration, yet consecutive fires that reburn populations before they reach maturity can cause local population contraction or extirpation. Understanding the environmental drivers of natural regeneration and mature tree survival, as well as when and how active reforestation should supplement natural recovery, is critical for managing fire-adapted species under altered fire regimes.

This thesis uses Baker cypress (Hesperocyparis bakeri [Jeps.] Bartel), a rare …

Aquatic Habitat Use By Western Toads And American Bullfrogs In California’S Central Valley: Effects Of Local Habitat Characteristics And Landscape Composition, Kaitlyn Breana Hernandez

Cal Poly Humboldt theses and projects

Drastic losses of historic wetland habitat in the California Central Valley have caused widespread population declines and extirpations of aquatic breeding amphibians. Efforts to restore wetland habitat have included private land, which represents 70% of land tenure in the Central Valley. Management of these restored wetlands is focused on waterfowl with the assumption that they will provide habitat for native herpetofauna. This study used combined surveys to test the assumption that restored wetlands on private land provide habitat for native amphibians with the western toad (Anaxyrus boreas) as a case study. The objectives were to use environmental DNA …

Early Successional Response To Stream Daylighting In Redwood National And State Parks, Orick, California, Rylee Rawson

Cal Poly Humboldt theses and projects

In-channel stream restoration projects can act as both disturbance and recovery mechanisms, disrupting existing conditions to reestablish the hydrologic and ecological processes that sustain stream ecosystems. These interventions initiate successional trajectories in which channel form and biological communities reorganize over time. In Redwood National and State Parks (California, USA), the Redwoods Rising initiative restores buried headwater channels by recontouring legacy logging roads and daylighting streams buried by Humboldt crossings. Using the National Park Service Klamath Network wadable stream monitoring protocol, I evaluated nine restored reaches spanning one to five years post-restoration, alongside old-growth reference reaches and available pre-treatment data. I …

Coho Salmon (Oncorhynchus Kisutch) Response To Habitat Restoration Through Space And Time: Monitoring Growth, Survival, And Movement In A Restored Habitat Network, Carter Perez Adamson

Cal Poly Humboldt theses and projects

Despite the decline of coho salmon (Oncorhynchus kisutch), Prairie Creek, a tributary of Redwood Creek in northern California, remains a coho salmon stronghold. The restoration of juvenile coho rearing habitat was identified as a recovery objective in this watershed by the National Marine Fisheries Service. Among the restoration projects addressing this objective are an ongoing project on Prairie Creek and its tributary Skunk Cabbage Creek, and a prior effort on Strawberry Creek (a tributary of Redwood Creek’s estuary). Standard monitoring methods for assessing project success have limitations and may be improved by measuring salmon use of restored habitats …

Relational Approaches To Fire Resilience: Designing A Living Shaded Fuel Break With Indigenous Knowledge Systems, Natalee Nicole Lopes

Cal Poly Humboldt theses and projects

Escalating wildfire severity across California reflects not only changing climatic conditions but also the cumulative impacts of colonial land management systems that have disrupted Indigenous fire stewardship and altered forest structure and function. This project examines how wildfire mitigation infrastructure, specifically linear fuel treatments, such as fuel breaks, can be reimagined through Indigenous relational frameworks of care-based land stewardship. Grounded in Happy Camp (Athithúfvuunupma), California, within the ancestral homelands of the Karuk Tribe along the mid-Klamath River, this research is carried out in collaboration with Karuk knowledge holders, centering Indigenous knowledge systems and priorities.

Using a mixed-methods approach that integrates …

Succession And Alpine Plant Habitat Of A Recently Deglaciated Foreland In The Trinity Alps, California, Sebastian Evans

Cal Poly Humboldt theses and projects

The recent extinction of the Salmon Glacier in the Trinity Alps, California, provides a rare opportunity to examine post-glacial succession in a region of high endemism experiencing climatic stress. Using a chronosequence spanning ~150 years since deglaciation, I investigated (1) vegetation successional trajectories in the foreland, (2) whether the foreland functions as a climate refugium for alpine specialists, and (3) whether facilitative interactions between the dominant vascular plant (DVP) and bryophytes occur and persist through succession. I surveyed 100 plots across 11 transects within the foreland and 40 randomized plots in adjacent ecosystems. Successional patterns and refugial potential were analyzed …

From Theory To Ecosystems: Exploring Biodiversity And Ecosystem-Function In Pitcher Plant (Darlingtonia Californica) Microbial Communities, Gavin Sutter

Cal Poly Humboldt theses and projects

The relationship between biodiversity and ecosystem-function (BEF) has been a central focus of ecological research for the last three decades, yet the role of space in shaping BEF relationships remains relatively unknown. Research has shown that community diversity and composition can strongly influence productivity, nutrient cycling, and decomposition, but much of this work treats communities as spatially isolated, leaving open how dispersal and other spatial processes shape BEF at broader scales. To begin addressing this gap, I used microbial communities inhabiting Darlingtonia californica pitcher plant leaves to evaluate how spatial organization and successional change shape biodiversity and ecosystem-functioning. I quantified …

Urban Trees In The Urban Hydrology Cycle, Jorge R. Vera

Theses, Dissertations and Capstones

In urban forestry, trees provide numerous economic, ecological, and aesthetic benefits to the surrounding community. One of their key roles is intercepting stormwater runoff, reducing flooding risk, and improving water quality. Chapter 1 of this thesis characterized the ecological and economic contributions of urban trees on the Marshall University campus and provides guidance on their management and conservation in the future. Chapter 2 examined how urban tree species modify the urban hydrology cycle by altering the volume and timing of precipitation delivery. In Chapter 1, we inventoried 114 acres of Marshall University's main campus in Huntington, WV, from June 28 …

Are There Enough Bees? Assessing Pollen Limitation In Native Eastern Washington Prairie Forbs, Alejandro Torres

EWU Masters Thesis Collection

Prairie ecosystems in eastern Washington have been severely degraded due to agricultural intensification, urbanization, and habitat fragmentation, leaving less than 0.1% of native prairie intact. As a result, these ecosystems have become some of the most endangered in the United States. One group of essential pollinators is the bees, which facilitate plant reproduction and contribute to the long-term persistence of restored plant communities. However, despite the presence of at least 257 native bee species in the region, the impacts of habitat fragmentation and restoration on pollinator activity and plant-pollinator networks remain poorly understood. For this study, we worked across Eastern …

Assessing Forest Health And Small Mammals After A Limited Suppression Wildfire In Colville National Forest, Samantha J. Kennel

EWU Masters Thesis Collection

In the face of a wildfire crisis, there is a growing need to expand the pace and scale of forest treatments, including the strategic use of wildfire as a management tool. However, ecological outcomes remain uncertain in forests shaped by more than a century of fire suppression. The 2022 Thor Fire, a managed wildfire in the Colville National Forest, Washington, provides an opportunity to evaluate forest health in a relatively understudied system. Using a control–impact design, I established 100 monitoring plots in burned and adjacent unburned forests to assess how wildfire and fire severity influence forest structure, fuels, and tree …

Dietary Ecology Of Waterfowl Nest Predators In The Prairie Pothole Region: Evaluating Resource Use From Species To Individual Using Stable Isotope Analysis, Grace Barthelmess

EWU Masters Thesis Collection

Nest predation is a primary factor influencing waterfowl productivity in the Prairie Pothole Region (PPR) of North America. Although this system is intensely managed and studied, research has largely focused on how predators affect waterfowl populations, with comparatively little attention to how predators actually rely on waterfowl as a resource. Understanding predator feeding ecology is therefore critical for informing effective management strategies and interpreting the complexity of predator-prey interactions on this landscape. My objective overall was to elucidate the feeding ecology of nine focal predator species commonly associated with waterfowl nest predation in the Aspen Parkland Ecoregion of the PPR: …

Mapping The Distribution Of The Common House Mosquito (Culex Pipiens) In The City Of Spokane: Mosquito Breeding Sites And Blood Meal Analysis, Oluwapelumi Hannah Sonoiki

EWU Masters Thesis Collection

Mosquitoes in the genus Culex are the primary vectors of West Nile virus (WNv) in the United States, with Culex pipiens and Culex tarsalis serving as the dominant species in Spokane. In urban environments, catch basins and other artificial water bodies that collect stagnant and polluted water provide favorable breeding habitats for Culex pipiens. This study aimed to assess the spatial distribution and seasonal dynamics of Culex larvae in Spokane. We examined 303 randomly selected catch basins in the summer of 2025, and at each site we recorded the presence or absence of water, water depth, and mosquito larval …

Relationships Between Benthic Macroinvertebrate Communities And Environmental Conditions In Headwater Tributaries Of The Pend Oreille Lake Watershed, Idaho, Devlin Mee

EWU Masters Thesis Collection

Benthic macroinvertebrates are widely used indicators of stream condition, and their communities respond to thermal and land-use conditions in headwater streams. This study characterized macroinvertebrate biomass, community composition, and diversity across forested headwater tributaries of the Pend Oreille lake watershed in northern Idaho, and examined their relationships with summer temperature, land development, and road proximity. Samples from thirteen streams collected in 2024 were identified to genus, measured, and converted to estimated biomass using published length–mass regressions; ten streams had recovered temperature loggers. Because environmental variables were characterized once per stream, all analyses were conducted at the stream level.

Estimated total …

Analysis Of Alternative Oxidase In Non-Angiosperm Plants, Anissa M. Francis

Theses and Dissertations (Comprehensive)

Alternative oxidase (AOX) is a mitochondrial terminal oxidase that provides an alternative pathway for electron transport, allowing plants to maintain respiratory flexibility under environmental stress conditions. While AOX has been extensively studied in angiosperms (flowering plants), little is known about its distribution and conservation in non-angiosperms. Based on gaps identified in the literature, this thesis investigated the distribution of AOX across non-angiosperm plant lineages. Although AOX is present in many green algae and is therefore considered to be an ancient protein that originated before the existence of non-angiosperm plants, it remains unclear whether the protein is uniformly distributed across these …

Detecting Habitat Use Of Three Endemic Freshwater Fish Of Far Southwestern Australia, Paige Rhiannon Wilson

Theses: Doctorates and Masters

The wetland ecosystems of far southwestern Australia face multiple threats including land clearing, historical cattle grazing, changed hydrology, extensive groundwater extraction, and changed fire regimes. Each is exacerbated by a drying climate, and together they provide a significant challenge to the on-going survival of the freshwater fish in this region particularly fish that are endemic, range restricted, and therefore, more likely to be more vulnerable. This study focuses on three such species: Galaxiella munda, Galaxiella nigrostriata and Lepidogalaxias salamandroides, the latter two of which are known to aestivate.

In order to make conservation decisions regarding management of wetlands for …

Moth Assemblages Of Fort Leonard Wood, Missouri, Kimberly Anne Steese

Masters Theses

"Moths (Lepidoptera) are ecologically important insects that function as herbivores, pollinators, prey resources, and indicators of ecosystem integrity. Despite their ecological significance, moth communities in the Missouri Ozarks remain poorly documented, particularly within military-managed landscapes. This thesis examined patterns of moth diversity, assemblage structure, and environmental drivers at Fort Leonard Wood (FLW), Missouri, while also assessing the distribution and genetic diversity of species within the genus Papaipema.

Field surveys were conducted from 2019 through 2021 across prairie, savanna, forest, and restoration habitats at FLW using UV bucket traps, sheet trapping, and targeted larval surveys. Across all sampling efforts, 29,116 individual …

Assessing The Effects Of Theta Transcranial Alternating Current Stimulation During Working Memory Performance On Theta-Gamma Phase Amplitude Coupling In Healthy Adults, Samantha M. Zimmer

UNF Graduate Theses and Dissertations

Transcranial alternating current stimulation (tACS) is an emerging form of noninvasive brain stimulation with substantial effects on behavior and brain function due to its ability to entrain endogenous neural oscillations. Working memory is a cognitive process that involves holding onto information that is no longer present in the environment and manipulating or reorganizing information for immediate use. Theta oscillations are critical for the functionality of the frontoparietal network and are involved in strengthening communication and coordination between frontal and parietal brain regions, especially during working memory processing. TACS applied at the theta frequency, targets theta oscillations, strengthens network communications, and …

The Importance Of The Proline Residue In The Ldiapa Motif Of Cholera Toxin, Akash Bharathan

Honors Undergraduate Theses

Cholera is currently on its seventh global pandemic, disproportionately affecting developing countries. Vibrio cholerae, the bacterial etiologic agent, is able to cause infection by adhering to intestinal epithelial cells and secreting cholera toxin (CT), an AB-type exotoxin that dysregulates signaling pathways. Previous research by the Teter lab has shown that Hsp90, a host cytosolic chaperone, is required for CT to enter the cytosol where it produces toxic effects. Hsp90 binds to CT at two specific motifs, RPPDEI and LDIAPA. The prolines in the RPPDEI motif are essential for binding, but the proline in the LDIAPA has yet to be …

Aquatic Invasive Species Survey And Treatment On Lake Umatilla And Lake Celilo 2024-2025 Report, Jacob Rose, Gabriel E. Campbell

Center for Lakes and Reservoirs Publications and Presentations

Flowering Rush (Butomus umbellatus) is an invasive aquatic plant in the Pacific Northwest that threatens salmon habitat. The Center for Lakes and Reservoirs staff surveyed for this and other aquatic species from 2024 and 2025 in the Columbia River in Lake Umatilla and Lake Celilo. This document summarizes their survey efforts including their protocols, data, and small-scale removal efforts.

A Note On The Novel Passive Sampling In Environmental Dna (Edna) Collection Technique To Detect Yellow Mud Turtle (Kinosternon Flavescens): Field And Laboratory Approaches In Turtle Edna, Sakib Tahmid Rishan, Richard J. Kline, Md. Saydur Rahman

School of Integrative Biological & Chemical Sciences Faculty Publications

Environmental DNA (eDNA) is a powerful and sensitive method for monitoring aquatic and terrestrial organisms, offering insights into their distribution. Usually, eDNA is collected using a filter membrane through water filtration. However, the passive eDNA method can simplify sampling and broaden its scope, offering new insights for ecological surveillance. This study examined the efficacy of six different membrane filters in detecting the yellow mud turtle (Kinosternon flavescens) through a novel passive eDNA method. After being immersed in water for 12 h, only the mixed cellulose ester (MCE) membrane filter consistently demonstrated specific polymerase chain reaction (PCR) amplification using …

Single-Cell Transciptomic Analysis Reveals Age-Related Remodeling Of Brain Endothelial Cells, Hai Duc Nguyen, Summer Siddiqui, Diana G. Bohannon, Robert V. Blair, Hong-Wen Deng, Alexandre Prat, Woong-Ki Kim

Microbiology & Molecular Cell Biology Faculty Publications

Blood–brain barrier (BBB) integrity naturally declines with age. Brain endothelial cells (ECs) and pericytes (PCs) form the BBB, and aging impairs tight junctions, likely via altered PC-to-EC signaling. However, the molecular mechanisms underlying this impairment remain unclear. Using single-cell RNA sequencing, we profiled 68,316 brain ECs expressing 15,564 genes from young and old mice. Unsupervised clustering and annotation revealed five distinct EC subtypes—Capillary EC1, Capillary EC2, Arterial EC, Venous EC1, and Venous EC2—defined by marker genes Mfsd2a, Plvap, Bmx, Nr2f2, and Vcam1, respectively. Aging shifted EC subtype distribution, with reduced Capillary EC1 (45% vs. 57%) and increased Arterial …

Reinterpreting Stochastic Optimal Control Under Ecological Uncertainty: Inferring Decision Urgency From Vegetation Biomass Dynamics, Komi Mensah Agboka, Tobias Landmann, Elfatih M. Abdel-Rahman

All Peer-Reviewed Publications

Stochastic optimal control provides a rigorous framework for systems subject to uncertainty, yet its operational use in ecological crisis contexts remains limited by interpretability. We reinterpreted a stochastic control formulation in which selected parameters emerged as indicators of decision urgency rather than normative preferences. Vegetation biomass was modeled as a stochastic stock subject to nonlinear loss driven by feeding pressure (e.g., desert locust activity) and multiplicative noise, with uncertainty represented by a time-varying volatility term that integrated extreme rainfall anomalies and conflict-related disruption. Rather than prescribing an optimal policy, we inverted the closed-form solution of the control problem to infer …