Digital Commons Network^™

The Influence Of Dna Methylation On Both H2a.Z Nucleosome Dynamics And The Zinc Finger And Homeoboxes Transcription Factor Family, Rochelle M. Shih

Student Theses and Dissertations

DNA methylation represents a widespread and heritable modification that exerts substantial influence on chromatin organization and function in many eukaryotic genomes. The presence of DNA methylation has been strongly linked to transcriptional regulation, in both activating as well as repressive ways, though exactly how a small chemical mark can enact such drastic and seemingly opposing effects on gene expression remains an open question. In my thesis work, I describe the influence DNA methylation has on two classes of chromatin proteins, the histone variant H2A.Z and the Zinc Finger and Homeoboxes (ZHX) family of transcription factors. DNA methylation and H2A.Z preferentially …

The Reinnervation Of Sensory Hair Cells Following Lesion Of The Lateral Line Cranial Nerve, Rohan S. Roy

Student Theses and Dissertations

Loss of hearing is a prevalent sensory pathology in the United States and worldwide. A significant proportion of deafness is attributed to sensorineural hearing loss, which often involves damage and retraction of the nerve fibers that relay auditory information between the mechanosensitive hair cells of the inner ear and the brain. The restoration of physiologic hearing would require the regeneration of nerve fibers back into the sensory epithelium of the cochlea, followed by the reinnervation of appropriate hair cell targets. Nerve regeneration studies in humans and other mammalian models are lacking due to the limited accessibility of the inner ear. …

Structural And Functional Studies Of The Sars-Cov-2 Niran Domain, Gabriel I. Small

Student Theses and Dissertations

The COVID-19 pandemic was a dramatically disruptive event, wreaking devastation across the globe and radically impacting nearly every facet of human life. For the first time, a significant amount of global money and attention was directed at coronavirus (CoV) research, propelling the field to the forefront of biomedical research. This allowed for the rapid development of a suite of vaccines and antivirals targeting SARS-CoV-2 that aided in ameliorating the effects of the pandemic. SARS-CoV-2 was the third CoV zoonosis of the 21st century and is now endemic. Global understanding of the threats posed by CoVs circulating among animals in the …

Dining In The Dark Atp Sensing In The Female Aedes Aegypti Mosquito, Katherine Adriana Rosas Villegas

Student Theses and Dissertations

Blood feeding is fundamental for female Aedes aegypti mosquitoes, which require this protein-rich meal for egg development. After being attracted to a human host by sensory cues such as body odor, body heat, and carbon dioxide exhaled in human breath, the mosquito lands and probes under the skin to feed on blood. Probing uses the stylet, a sharp appendage ensheathed in the mosquito labium that punctures the skin and is highly specialized for blood ingestion. Previous work from the lab with GCaMP calcium imaging of stylet neurons showed that approximately 50% of stylet neurons respond to blood, and that these …

Rewilding The Coral Reefs, Magali Rodriguez, Luciana Alvillar

Posters - 2026

Coral reefs are among the most valuable ecosystems globally, contributing an estimated $10 trillion annually and supporting hundreds of millions of people through food, livelihoods, and coastal protection. (NOAA 2026) They also provide critical habitat for diverse marine species. However, reefs are rapidly declining due to rising ocean temperatures, acidification, pollution, invasive species, and physical disturbances. Globally, 30–50% of coral reefs have already been lost(NOAA 2026), and without intervention, many may face extinction by the end of the century. Rewilding and restoration efforts have become essential strategies to address this crisis. Initiatives such as NOAA’s Coral Reef Conservation Program highlight …

Impacts Of Native And Nonnative Plant Species On Avian Community Diversity, Matty John Mackay, Steven Van Dang, Brandon Lee Corn

SPARK Symposium Presentations

Avian communities play an essential role in maintaining a healthy ecosystem dynamic by regulating insect populations, assisting in plant pollination, and seed dispersal. These contribute to the overall health of natural ecosystems and mitigate ecological disruptions that would arise in their absence. However, the introduction of non-native invasive plant species can interrupt the balance avian communities bring to ecosystems by reducing availability of food sources, altering habitat structures, increasing competition among individuals, and disrupting ecological relationships through rapid invasion of ecological spaces. Point count observations of birds at Shelby Bottoms Park, Nashville, Tennessee in areas dominated by non-native privet and …

Chronic Alcohol Consumption Reprograms Osteoclast Lineage Communications To Promote Osteoclastogenesis, Hami Hemati, Brianna M. Doratt, Ilhem Messaoudi

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Chronic alcohol consumption increases the risk of osteoporosis and fracture by disrupting bone remodeling, in part by enhancing osteoclastogenesis. However, the cellular mechanisms underlying this process remain incompletely defined. We analyzed scRNA-seq data from osteoclasts differentiated in vitro from bone marrow mononuclear cells obtained from macaques following 12 months of chronic ethanol or isocaloric control solution consumption. Module scoring, trajectory inference with generalized additive modeling (tradeSeq), and CellChat-based analyses of intercellular communication were applied to uncover ethanol-induced changes in metabolic reprogramming, lineage progression, and signaling network dynamics. Module scoring indicated metabolic reprogramming toward oxidative phosphorylation, with reduced glycolytic, migratory, and …

Assessing Radio Transmitter Weight Effect And Evaluation Of Northern Bobwhite Chick Survival In Coastal North Carolina, Autumn S. Randall, Theron M. Terhune Ii, Matthew T. Springer, Darin J. Mcneil

Forestry and Natural Resources Faculty Publications

Studies on the earliest life stages are essential to our ecological understanding of avian demography; however, monitoring technologies that allow tracking of small birds are still limited in a variety of ways. One critical limitation, until recently, has been the development of methods for attaching transmitters to young birds with precocial development (e.g., Galliformes, Charadriiformes, etc.). The modified suture technique offers a means to attach transmitters to precocial chicks while accommodating rapid growth characterized by birds with precocial young. Although the sutured transmitter method was developed for northern bobwhite (Colinus virginianus) chicks, there remain key unknowns regarding best …

Hyena Den Distribution, Human-Hyena Interactions And Mitigation Strategies In The Greater Amboseli Ecosystem, Katherine Elizabeth Davis Matczak

Honors Theses and Capstones

The Spotted Hyena (Crocuta crocuta) is a prevalent predator across Africa, recognized for their noticeable gait and powerful jaws which enable them to maul and scavenge on prey. Due to human population growth and encroachment onto wildlife territory, the overlap between human settlements and hyena den sites has become a growing concern, particularly regarding livestock predation. Livestock is intrinsic to livelihoods across Africa, with most individuals deriving their livelihoods from pastoralism. This paper provides an evaluation of den proximities to communities, hyena diet preference, human-hyena conflict/attitudes, and mitigation strategies used to protect hyenas, livestock, and the people. Results …

Current And Historical Occurrences Of Rare Coastal Plants In The Hampton Falls Salt Marshes, Liliana T. Chirichella

Honors Theses and Capstones

A botanical survey of two sites in the Hampton Falls saltmarshes was conducted to document the occurrences of a list of eleven endangered and threatened coastal plants listed in the Natural Heritage Bureau’s NH Rare Plant List. This survey was conducted in fulfillment of the NH Native Plant Protection Act to highlight and identify target species in an area where restorative runneling is to be implemented in order to restore proper hydrology and prevent further expansion of mega-pools in the Little River and Depot Road salt marsh sites, which have been subject to historical ditching and are a part of …

Exploring The Association Between Internal Body Temperature And Metabolic Status In Early Lactation Jersey Cattle, Alexandra Ferreri, Claira Seely

Honors Theses and Capstones

No abstract provided.

Generating Concentrated Ring Polymers Through Supercoiled And Open Circle Pdna Isoform Separation, Kelsey E. Hodsdon

Honors Theses and Capstones

DNA can serve as a model polymer due to its high uniformity compared to synthetic polymers. Plasmid DNA (pDNA) in particular offers a well-defined, low-dispersity model for studying topologically uniform systems of a cyclic nature. pDNA has unique isoforms known as open circle (OC) and supercoiled (SC). Using fast protein liquid chromatography (FPLC), specifically anion exchange chromatography (AEX), these OC and SC isoforms can be separated in order to access their unique material properties. Our lab was able to yield the pure SC isoform. However, OC isoform separation has been more challenging. Various difficulties arose during this process, including imaging …

Fecal Microbiota Transplantation Modulates Gut Microbiota And Lipid Metabolism To Reduce Diarrhea In Low-Birth-Weight Neonatal Calves, Zhanhe Zhang, Duo You, Donglin Wu, Xintong Li, Yang Jia, David L. Harmon, Ming Xu

Animal and Food Sciences Faculty Publications

This study aimed to elucidate the mechanism by which colonic fecal microbiota transplantation (FMT) improves lipid metabolism in low-birth-weight neonatal calves via modulation of the gut microbial ecosystem. In a completely randomized design, seventy newborn Holstein calves (1 day old, 32.63 ± 3.13 kg BW) were assigned to two groups (35 calves/treatment; 30 females and 5 males per group). A healthy calf was used as a fecal donor. A 10% fecal bacterial suspension was prepared and administered colonically (50 mL, twice daily for 5 days). All calves were fed the same milk replacer and weaned using a step-down protocol at …

Hidden In The Mist: Three New Species Of Salamanders Of The Genus Pseudoeurycea (Amphibia: Plethodontidae) From Oaxaca, Mexico, Eduardo A. Aguilar-Herrera, Salomón Sanabria-Urbán, Dulce L. Flores-Martínez, José David Gómez-Tapia, Misael Seba-Chacha, Leopoldo D. Vázquez-Reyes, Francisco A. Rivera-Ortíz, Patricia Ramírez-Bastida, Bruno Cortés-Ortiz, R. Alejandro Calzada-Arciniega, Etienne U. Avila-Ortega, Dónovan I. Víquez-Vega, Omar Hernández-Ordoñez, Christopher Blair, Víctor H. Jiménez-Arcos

Publications and Research

We describe three new species of lungless salamanders (Plethodontidae) in the genus Pseudoeurycea from Cerro Rabon, located on the eastern slopes of the Sierra Mazateca, Oaxaca, Mexico. This mid-elevation mountain, largely surrounded by warmer lowlands, remains relatively understudied. We present molecular and morphological evidence distinguishing the three new species from their congeners. According to our phylogenetic analysis, one of the new species belongs to the P. leprosa group, while the other two are members of the P. juarezi group. Notably, one of the new species represents the fourth worm salamander known from west of the Isthmus of Tehuantepec, and another …

The Role Of Body Composition In Predicting Fitness Performance Of Law Enforcement Officers, Taylor J. Parmentier

Theses and Dissertations

Law enforcement officers (LEOs) perform physically demanding tasks such as foot pursuits, lifting, carrying, dragging, and physical restraint. Effectively performing these duties requires significant muscular strength, endurance, and cardiorespiratory fitness. Lower fitness levels pose risks for reduced health, performance, slower recovery, increased fatigue, and potentially affecting decision-making under strenuous conditions. The purpose of this study was to identify the strongest predictor of physical fitness performance among active-duty LEOs. Body composition analysis and a comprehensive fitness evaluation of twenty-nine male LEOs (age: 36.2 ± 9.3 years, body mass (BM): 95 ± 13.7 kg, height: 1.8 ± 0.1 m) was completed. The …

Bpa: Effects Of The "Plastic Era", Bianca G. Pietrangeli

Student Work

Infographic review of sources of BPA and its impacts on human health with a focus on osteoporosis.

Trustworthy Multimodal Ai For Medical Imaging: Enhancing Diagnosis, Reasoning, And Human-Agent Interaction In Extended Reality, Jai Prakash Veerla

Computer Science and Engineering Dissertations

The transition from traditional microscopy to digital pathology has digitized diagnostic data, yet clinical workflows remain constrained by two-dimensional screens and passive, opaque analysis tools that fail to capture the spatial complexity of biological systems. While Foundation Models now promise to reason across histology and genomics, a critical disconnect persists between the richness of this data and the limited cognitive bandwidth of clinicians, who currently lack the immersive interfaces and trustworthy agents necessary to utilize it effectively. This dissertation presents a unified framework for "Embodied Agentic AI," establishing a pipeline that augments physician capabilities through immersive visualization, robust security, and …

Toxic Torts Are In Your Blood: An Examination Of Pfas And Tort Liability, Aiden M. Holden

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Abstract

Per- and polyfluoroalkyl substances (PFAS), are a group of chemicals widely manufactured for industrial and commercial applications in the past decade due to their remarkable stability. Despite the “useful” nature of PFAS in various applications, they represent one of the most profound environmental and public health crises of the modern era. The chemical stability of PFAS has made them almost completely resistant to natural degradation, earning them the moniker “forever chemicals.” PFAS forever haunt our plant, contaminating the soil, water, and the blood of approximately 95% of the United States population. As the scale of corporate malfeasance by chemical …

Know Fear: The Culture Of Fear In Community Conversations, Penelope Meredyk, Lotus Porte-Moyel, Fiona Carson, Cosley Bruno, Barrett Clement, Sophia Sproull, Morgan Davis

Undergraduate Theses, Professional Papers, and Capstone Artifacts

“K(no)w Fear” is an award winning piece of verbatim theater based on word-for-word interviews and collected research that's goal was to cultivate a connective and thoughtful community open to exploring what shapes us all: fear. Our GLI Capstone team gathered the resources for this theater piece by conducting interviews with a mixed demographic of people we know as well as strangers on the street, asking questions like: “How do you feel about death?” and “If fear were a place for you, where would it be?” With consent, their answers were used to build an immersive, verbatim theatre piece where actors …

Corals, Creation, And Conservation: Evaluating Human-Marine Relations Through Coral Reef Biology And Artistic Practice, Emma Gray Garman

Scripps Senior Theses

This thesis investigates the relationship between humans and marine ecosystems through two complementary lenses: scientific research and artistic practice. The biology chapter proposes a five-year coral reef monitoring study across four Queensland, Australia islands — two heavily visited and inhabited (Fitzroy and Green Islands) and two minimally visited and uninhabited (High and Woody Islands) — to assess whether human activity correlates with bleaching severity over time. Grounded in preliminary survey data from Green Island in 2024, which revealed approximately 54% of coral bleached or dead, the study hypothesizes that reefs adjacent to inhabited islands will exhibit greater bleaching severity, consistent …

Combinatorial Immunotherapy Drives Exhaustion In Tumor Antigen–Specific Cd8+ T Cells Within The Mouse Renal Tumor Microenvironment, Holly R. Stephens, Elizabeth Elkins, Jing Li, Zoey N. Swalley, Henry Nnaemeka Ogbonna, Pia Muri, Zachary Roberts, Francesca R. Dempsey, Md Akkas Ali, Md Hasanul Banna Siam, Richard Kirkman, Jianqing Zhang, Sunil Sudarshan, Daniel J. Tyrrell, Hubert M. Tse, Daniel L. Smith Jr, Lyse A. Norian

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Immunotherapies have greatly improved outcomes for patients with renal cell carcinoma (RCC), yet response rates remain suboptimal and the factors promoting therapy resistance versus sensitivity are incompletely understood. Currently, no preclinical model of orthotopic renal cancer exists that permits evaluation of tumor antigen–specific (TAS) CD8⁺ tumor-infiltrating lymphocytes (TILs). To address this deficiency, we developed a mouse renal cancer model that permits tracking of adoptively transferred TAS CD8⁺ T cells. Renca-LUC tumor cells were transduced to express tumor ERK (tERK), a model antigen expressing a one-amino-acid change from wild-type ERK, resulting in recognition by tERK/H-2K^d-specific DUC Thy1.1 …

Evaluating Sampling Strategies To Monitor Southern Fox Squirrel (Sciurus Niger Niger) Populations, Casey Robin Hitchens

Theses, Dissertations and Capstones

Reliable population monitoring is essential for evaluating wildlife translocations and informing conservation management, yet traditional monitoring approaches are often labor intensive and require prolonged sampling. This study evaluated marking techniques, survey methods, and population estimation approaches for monitoring a translocated population of southern fox squirrels (Sciurus niger niger) at Marine Corps Recruit Depot Parris Island, South Carolina, USA (MCRDPI). From January-June 2023, I conducted six live trapping and camera trapping sessions across five survey sites. I compared the performance of color-coded collars and tail shaves for individual identification and the recapture probability between live traps and camera traps. Logistic mixed-effects …

Nanobubble-Enabled Foam Fractionation To Remove Freshwater Microalgae And Microcystin, Yihan Zhang, Lili Li, Shan Xue, Tsung-Ta David Hsu, Anne C. Hurley, Meiyin S Wu, Xuezhi Zhang, Wen Zhang

Department of Biology Faculty Scholarship and Creative Works

Climate change and water pollution intensify algal blooms in natural lakes and reservoirs, leading to significant water quality challenges. Effectively removing excessive microalgae and algal toxins or micropollutants is essential for improving water quality, preserving aesthetic value, and protecting human health. This study evaluated the use of nanobubble-enabled foam fractionation for the removal of Microcystis aeruginosa and three microcystin congeners (MC-LR, MC-RR, and MC-YR). Foam was generated using air nanobubbles combined with surfactants—cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), and chitosan—to enhance foamability and stability. Among the tested surfactants, CTAB exhibited the highest foamability, yielding improved removal performance. When CTAB …

Znf16 Is A Nucleolar-Associated Protein That Regulates Expression Of Rdna And Cancer-Associated Genes, Chelsea L. George, Laura A. Espinoza Quevedo, Jason Paratore, Matthew J. Alcaraz, Arlene P. Levario, Yasir Rahmatallah, Galina V. Glazko, Nathan S. Reyna, Laura Diaz-Martinez

Biology Faculty Scholarship

ZNF16 (also known as HZF1 and KOX9) is a multi-C2H2 zinc finger protein first identified via its expression in human T-cells and shown to have a role in blood cell differentiation. ZNF16 was later shown to be ubiquitously expressed in a variety of fetal and adult tissues, suggesting a broader function. In this study, we confirm the ubiquitous expression of ZNF16 in a variety of cancer and non-cancer cell lines and show that ZNF16 depletion reduces cell viability in all cell lines tested. Furthermore, we show that ZNF16 localizes to the nucleolus in a transcription-dependent manner, interacts with the intergenic …

Cordgrass (Spartina Alterniflora) In Bio-Restoration Integrated Coastal Substrate (Brics): Restoring The New England Coastline., Kaitlyn Frances Shelley

UNH URC Open (2026 and after)

The Engineering for Agri-Environment Laboratory (EAE) is dedicated to developing nature-based solutions that enhance the resilience of agricultural and coastal ecosystems. The study involves current work (trial 2) underway in Spring 2026 and Summer 2026, integrating principles of sustainable engineering and plant science. BRICS, our Bio-Restoration Integrated Coastal Substrate, proposes a new way to reduce erosion, stabilize root structures, provide a critical habitat for wildlife, and promote vegetation throughout the coast.

Interactions Between Red Foxes And Coyotes Across Urban And Wild Habitats, Caroline E. Savage, Taylor S. Readyhough, Remington J. Moll, Jonathon D. Cepek

UNH URC Open (2026 and after)

Dens are critical to the life history of many mammal species, particularly when raising young. Excavating a den is energetically costly, so several species can use existing dens, and multiple species may inhabit the same den complex simultaneously. However, the extent of den sharing among urban mammals is understudied. Between March 2021, and June 2023, we used camera traps to monitor a multi-species den site in Cleveland, Ohio, USA. We documented den visitation and use by at least 11 mammal species, including red fox, coyote, raccoon, woodchuck, and Virginia opossum. Coyotes and red foxes both exhibited scent-marking at the den …

Soil Organic Carbon And Soil Health Property Responses To Land Management Across Topographic Positions, Mia D. Makovsky

All Graduate Theses, Dissertations, and Other Capstone Projects

uring the last ice age, ice sheets advanced and retreated across the upper Midwest, leaving behind a landscape characterized by poorly drained hummocky topography. Well-aerated soils have formed on uplands and lowlands are dominated by hydric soils. These wetlands and hydric soils have potential to store large quantities of carbon, particularly compared to the well-aerated uplands, but many have been drained and cultivated for decades. Conservation agriculture methods have emerged to protect and conserve soil health from negative effects due to cultivation.The purpose of this study is to determine how agricultural management practices and landscape properties (e.g, topographic position) affect …

Analyzing The Impact Of Sunscreen Pollutants On The Development Of Lytechinus Variegatus, Christina M. Lillenas, Chesney J. Anderson, Ann M. Buchmann

Nebraska Academy of Sciences: Programs and Proceedings

Lytechinus variegatus or the variegated sea urchin is found in shallow, tropical waters. Lytechinus variegatus can be found in sandy reef beds, and sea grass, covered in algae and debris to protect itself from the UV rays and predators. Sea urchins play a crucial role in coral reef ecosystems. Sea urchins follow an omnivore diet, feeding on algae and phytoplankton. In coral reef ecosystems, sea urchins eat unwanted algae, allowing coral reefs to continue growing and thriving. Without the presence of sea urchins in coral reef communities, algae can negatively affect the growth and success of coral reefs. Sunscreen serves …

Inferring Genes And Biological Systems Under Selective Pressure In The Secondarily Temperate Cryonotothenioids, Esteban Cabrera

Theses, Dissertations and Culminating Projects

Notothenioid fishes represent one of the most compelling examples of adaptive radiation driven by extreme environmental change, having diversified in the chronically cold waters of the Southern Ocean. While most cryonotothenioids evolved highly specialized cold-adapted characteristics, several secondarily temperate species later re-invaded warmer environments. These lineages provide a rare opportunity to examine evolutionary reversibility and the genomic mechanisms enabling escape from extreme specialization. In this study, we investigate two secondarily temperate species, Dissostichus eleginoides and Champsocephalus esox, comparing their protein-coding orthogroups to background sets from Antarctic cryonotothenioids and basal temperate teleosts. Using phylogeny based evolutionary models (BUSTED, aBSREL, and RELAX) …

A Trajectory Of Zostera Marina (Eelgrass) Ecosystem Recovery: Pre- And Post-Hurricane Sandy Degradation In Barnegat Bay, New Jersey, Edgar A. Medina

Theses, Dissertations and Culminating Projects

Hurricane Sandy struck Barnegat Bay, New Jersey in 2012 and obliterated the benthic marine ecosystem with extensive damage to Zostera marina meadows. Previous genetic assessments of Barnegat Bay Z. marina in 2008 revealed low heterozygosity and low connectivity between populations. The great disruption caused by Hurricane Sandy motivated us to investigate the long-term effects of the storm on the genetic condition of the eelgrass populations in the year 2021. The investigation revealed improvements in heterozygosity, inbreeding, and connectivity. These findings drove us to investigate the genetics of Barnegat Bay eelgrass populations in the intervening years of 2013 and 2017 with …