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Long Term Changes In Elk Group Size And Composition (2002-2023) In Relation To Wolf Hunting Sucess, Kennedy Price

Undergraduate Honors Capstone Projects

Elk (Cervus elaphus) in Yellowstone National Park exist within a predator-prey system shaped by long-term interactions with wolves (Canis lupus). This predator-prey system represents an important model for understanding how predation influences ungulate behavior and social organization. Predators affect prey behavior through direct and indirect interactions and may cause long-term changes. What this study strives to answer is if predator success can have long term effects on prey grouping behavior and age-sex structure. In a previous study, wolves were found to be most successful hunting medium sized group of elk (20-49 individuals) in Yellowstone National Park. …

Developing Training And Streamlining Training Management Process For Smithfield Fire Department Employees In Aha, Naemt, And Aap Certifications, Michael Christopher Popa Ii

Undergraduate Honors Capstone Projects

The rapid operational expansion of the Smithfield Fire Department and EMS (SFD)—marked by a 300% increase in call volume and a transition to a fully professional, independent paramedic service—exposed critical systemic inefficiencies in clinical preparedness and personnel credentialing. This project addresses the "HALO Paradox," wherein high-acuity, low-occurrence emergencies require expert-level proficiency that cannot be maintained through call volume alone. Historically, the agency relied on fragmented, external continuing education (CE) that incurred high financial costs, lacked equipment standardization, and created administrative bottlenecks during biannual recertification cycles. The central objective of this capstone was to engineer a sustainable, in-house training infrastructure that …

The Hitchhiker's Guide To The Host: Fleas And The History Of Pikas In North America, Josh Pletcher

All NMU Master's Theses

Parasite phylogenetics can offer windows into the cryptic evolutionary history of hosts, often highlighting events that are not recorded by the phylogenetics of the host. Pikas (small lagomorphs) host a diverse parasite community comprising endoparasites (e.g., coccidians, nematodes, and cestodes) and ectoparasites (e.g., fleas and mites). Phylogenetic analysis of pika endoparasites has revealed pikas evolved in Asia and colonized North America in two waves, and that contact between these two pika lineages allowed parasites to be transferred from one lineage to another and to survive the extinction of their original host. To uncover these cryptic histories, understanding the biogeography of …

Behind The Smile: A Qualitative Study On Dentist Burnout And Workplace Satisfaction In Cache Valley, Luke Thomas Hatch

Undergraduate Honors Capstone Projects

This qualitative research study examined satisfaction, stressors, and burnout among dental professionals in Cache Valley, Utah. Eight practitioners at various points in their careers were interviewed with a range of specialties including general dentistry (n = 3), pedodontics (n = 3), oral and facial surgery (n = 1), and orthodontics (n = 1). Analysis of the interviews showed that level of clinical autonomy was a primary factor influencing burnout levels. Stress levels in more experienced practitioners and owners tended to be lower than those who worked in multiple practices (multi-doctor) or with corporations. Individuals in these environments reported higher levels …

Population Structure And Connectivity Of Atlantic Nurse Sharks (Ginglymostoma Cirratum) In The Bahamas And Outlying Areas, Baylie A. Fadool

School of Natural Resources: Dissertations, Theses, and Student Research

Understanding animal ecosystem dynamics and behavior at the individual and population levels is essential for identifying life-history patterns and important areas for management and conservation. Marine fish species often face minimal geographic barriers to movement, but environmental (temperature, salinity, current) or intrinsic barriers (philopatry, site fidelity) can dictate gene flow. Atlantic nurse sharks (Ginglymostoma cirratum) are an International Union for Conservation of Nature (IUCN) listed ‘vulnerable’ species and present a unique opportunity to investigate patterns of population structure and connectivity in large-bodied sharks. They are benthic and display reproductive philopatry, with recent work suggesting they have the capacity …

Exploring Student Sensemaking During A Collaborative Biology Lab, Rebecca Kollipara

School of Natural Resources: Dissertations, Theses, and Student Research

Collaborative learning environments offer valuable opportunities for students to construct scientific understanding through dialogue, yet the conditions that support sustained conceptual reasoning in group settings remain incompletely understood. While prior research has identified discourse moves associated with productive collaboration, less attention has been paid to how those moves function together across turns and conceptual boundaries, or how individual student characteristics shape their emergence. This thesis examines collaborative sensemaking in an undergraduate General Biology II course, drawing on two complementary analyses of student discourse during a bryophyte life-cycle modeling activity.

Chapter 1 investigates how student groups participated in sensemaking and how …

Gaining Mussel: Evaluating Reintroduction Success Of Native Freshwater Mussels In Nebraska And Eastern Wyoming, Travis A. Moore

School of Natural Resources: Dissertations, Theses, and Student Research

Freshwater mussels are one of the most imperiled groups of organisms in North America, with over two-thirds of species considered threatened, endangered, or of special concern. These long-lived bivalves are essential to stream ecosystems, acting as filter feeders that improve water quality and as indicators of aquatic health. In Nebraska and Wyoming, native mussel populations have declined sharply due to habitat modification, pollution, and reduced connectivity. Two species of particular concern are the Plain Pocketbook Lampsilis cardium, listed as Tier I at-risk in Nebraska, and the Fatmucket Lampsilis siliquoidea, a Tier II at-risk species. In response, state agencies …

The Impact Of Glucose Exposure On Organic Cation Transporters (Oct) Function In A Corneal Epithelial Cell Line, David A. Garza

Theses & Dissertations

Organic cation transporters (OCT) facilitate the movement of neurochemicals and medications, contribute to the removal of cellular waste, and facilitate neuronal communication. Three subtypes of these high-capacity, low-affinity transporters (OCT1, OCT2, and OCT3) are differently expressed in peripheral tissues such as the liver, kidneys, as well as the central nervous system. OCTs are also expressed in ocular tissues. Chronic hyperglycemia is a complication commonly associated with diabetes that can lead to ocular diseases such as diabetic keratopathy, which affects the cornea. In this project, we expose immortalized human corneal epithelial cells (HCE-S) to various glucose concentrations to study their impact …

Honeybee Colony Response To Probiotic Supplementation And Grazing Practices, Katya M. Busenitz

Department of Entomology: Dissertations, Theses, and Student Research

Honeybee health is a complicated issue. Studies were conducted to scale honeybee (Apis mellifera) health from the microbiome to the landscape. Colony health parameters were measured in response to probiotic administration. Colony capped brood production, colony summer weight gain, and Varroa mite incidence were measured. I found that applications of over-the-counter probiotics did not improve the measured parameters of honeybee health compared to a control group. Results suggest that probiotic application was not beneficial but positive benefits may depend on application method, brand, or strain of probiotics used. I also examined how colony health metrics responded to different …

Bio 23 Human Anatomy & Physiology I Syllabus, Rujin Tian

Open Educational Resources

The syllabus for BIO 23 contains a course description, course objectives, course readings divided by week and a link to an OER textbook.

Bio 24 Human Anatomy And Physiology Ii, Rujin Tian

Open Educational Resources

The syllabus for BIO 24 contains a course description, course objectives, course readings divided by week and a link to an OER textbook.

Inosine Accelerates Cancer Cell Killing By The Experimental Antifolate Ucp1162: Implications For Novel Combination Treatments, Reshma Ramesh, Charles Giardina, Alan Kuo

Honors Scholar Theses

Antifolates disrupt one-carbon metabolism (OCM), which stops nucleotide synthesis and therefore slows down cell proliferation. Our experimental antifolate, UCP1162, stops de novo purine synthesis and thymidine synthesis by targeting the OCM pathway. We investigated whether the downstream metabolites of OCM pathway: methionine, thymidine, and inosine, affect the cell death caused by UCP1162 using MV4-11 leukemia cells. Thymidine was found to partially rescue MV4-11 cells from UCP1162, in contrast, inosine was found to accelerate the cell death caused by UCP1162. These cytotoxic effects of UCP1162 alone and in combination with inosine were irreversible, as removal of treatment did not restore cell …

Validation Of A Real-Time Pcr Laboratory Developed Test For The Detection Of Pneumocystis Jirovecii In Bronchoalveolar Lavage Samples, Caitlyn Moon

Honors Scholar Theses

Pneumocystis jirovecii is an opportunistic, host-obligate fungal pathogen responsible for causing P. jirovecii pneumonia (PJP) in humans. PJP is treated with trimethoprim-sulfamethoxazole (TMP-SMX), a strong broad-spectrum antibiotic that carries risk of renal and hepatotoxicity. Therefore, rapid diagnosis or exclusion of PJP is crucial to ensure PJP-positive patients receive effective treatment and PJP-negative patients avoid unnecessary antibiotics. Unlike most microorganisms, P. jirovecii cannot be cultured, presenting complications for the typical methods of diagnosing fungal pathogens. Historically, the gold standard for diagnosis was direct microscopic examination with stains such as methenamine silver and calcofluor white, but this diagnostic technique requires highly skilled …

The Development Of A Crispr-Based Calibrated Functional Assay For Classifying Pathogenicity Of Msh2 And Msh6 Variants In Lynch Syndrome, Olivia N. Amodeo

Honors Scholar Theses

Lynch syndrome is a hereditary disease caused by the inheritance of a mismatch repair gene variant. Individuals with this condition are predisposed to cancer development, most commonly colorectal cancer. Current guidelines for variant classification are based on numerous evidence categories, including functional evidence. However, many novel clinical variants are not well characterized, and evidence is difficult to obtain if functional assays are not calibrated.

To address this, our lab created a calibrated functional assay that calculates an odds of pathogenicity score for MSH2 and MSH6 gene variants that can be used as evidence for classifying variants of uncertain significance. This …

Reversible, Light-Controlled Inhibition Of Stim1-Mediated Calcium Signaling Using Photoswitchable Ml-9 Derivatives In Jurkat-Lucia Nfat Reporter T Cells, José L. Matute Gálvez

Honors Scholar Theses

Store-operated calcium entry (SOCE) through calcium release-activated

calcium (CRAC) channels is the dominant pathway for sustained Ca2+ influx in

non-excitable cells and is essential for T cell activation, driving downstream

signaling through the calcineurin-NFAT transcriptional axis. Dysregulation of

SOCE produces multi-systemic disease phenotypes, establishing that precise,

spatiotemporally controlled modulation of this pathway is a fundamental

therapeutic requirement. ML-9 is one of the only characterized small molecules

capable of targeting SOCE upstream at the level of STIM1 localization, but lacks

the selectivity, pharmacokinetic properties, and spatiotemporal control necessary

for therapeutic development.

This work describes the design, synthesis, and biological evaluation of …

Examining Pathogenic Vibrio Abundance And Environmental Controls Across Macroalgae, Sediment, And Oysters, Alexandra H. Geisser, Abigail K. Scro, Roxanna Smolowitz, Robinson W. Fulweiler

Arts & Sciences Faculty Publications

Climate change is accelerating the geographic spread and abundance of pathogenic Vibrio species in coastal waters, posing growing threats to ecosystems and human health. We investigated the abundance and pathogenic gene presence of V. vulnificus and V. parahaemolyticus across three common estuarine substrates (macroalgae, sediment, and oysters) in a temperate estuary. Macroalgae consistently harbored the highest Vibrio counts in conjunction with elevated pathogenic gene frequencies, while oysters contained the lowest. Environmental conditions related to Vibrio abundance were substrate and Vibrio species specific. Temperature and salinity were most closely related to pathogenic Vibrio abundance in oysters. In contrast, pathogenic Vibrio abundance …

Biohybrid Robotic Jellyfish For Swimming-Enhanced Vertical Ocean Profiling, Kelsi M. Rutledge, Sean Colin, John H. Costello, Noa Yoder, Simon R. Anuszczyk, Kelly R. Sutherland, Brad L. Gemmell, John O. Dabiri

Arts & Sciences Faculty Publications

Ocean monitoring is essential for understanding climate change and marine ecosystem dynamics, yet achieving comprehensive global coverage remains a challenge in oceanography. Current technologies face limitations in cost, power, hardware, and depth capacity that restrict widespread monitoring capabilities. Here we show that biohybrid robotic jellyfish (Aurelia aurita) can serve as autonomous vertical ocean profilers by integrating microcontrollers with positively buoyant sensor payloads, achieving controlled vertical-profiling capabilities. Laboratory experiments demonstrated repeatable up–down trajectories, quantified force balance limits, and identified predictable, size-dependent descent swimming speeds. Field deployments in Massachusetts coastal waters and the open ocean off the Florida Keys demonstrated field operation …

Optimizing Citriculture In South Texas With Endemic Hlb, Ruth Lilia Galan

Theses and Dissertations

The Texas citrus industry has a long history of resilience, rebounding from environmental and economic disasters. Over the last decade it has endured several hurricanes, freezes, pests and diseases. Huanglongbing (HLB), a graft-transmissible disease caused by Gram-negative bacterium Candidatus Liberibacter asiaticus which is vectored by the Asian citrus psyllid (ACP) (Diaphorina citri Kuwayama), is an endemic citrus disease posing serious threat to the long-term viability of the industry. Rapid production of high quality nursery trees and effective protection of young citrus transplants from HLB are critical for long term sustainability of citriculture in this region. Studies were performed to improve …

Phenotypic Contribution And Regulation Of Vibrio Parahaemolyticus Cold-Shock Protein Homologue Vp1889, Oleg Borisovich Ermolinsky

Theses and Dissertations

Vibrio parahaemolyticus is the primary causative agent of a form of gastroenteritis called vibriosis commonly acquired from ingestion of raw and undercooked seafood. The primary mitigation of seafood-borne illnesses is immediate storage on ice following catch, which inhibits bacterial metabolism and replication. Mesophilic bacteria such as V. parahaemolyticus induce expression of cold-shock proteins in a process named the cold shock response, which facilitates survival at low temperatures. V. parahaemolyticus bears 3 homologues of the major Escherichia coli cold-shock protein CspA, an RNA chaperone. Induction of E. coli CspA at low temperatures depends on autogenous modulation by its mRNA structure, which …

The Effect Of Drought On Avian Migratory Patterns And Stopover Through Texas And Louisiana, Ian Becker

Theses and Dissertations

Extreme weather events pose a massive threat for native ecosystems. As precipitation and temperature patterns are altered due to climate change, drought may become an incredibly severe event on the landscape. Drought directly impacts resources that migratory birds need during stopover en route to breeding and non-breeding grounds. Here, I quantify the impacts of drought on migration through Texas and Louisiana using 26 years of weather surveillance radar data. To understand how drought interacts with the local environment to impact migration, I tested 4 hypotheses displaying drought’s ability to impact migrants in conjunction with climatic and habitat level characteristics. Fall …

Ethanol State-Dependent Learning In The Honey Bee (Apis Mellifera), Ethan Neil Govea

Theses and Dissertations

Ethanol disrupts neural circuits involved in addiction and withdrawal, yet the molecular mechanisms underlying these effects remain poorly understood. One possibility is that ethanol alters an animal’s perceptual or internal state, causing a stimulus learned while intoxicated to be processed differently when the animal is sober. This study examined how ethanol exposure affects odor learning, recall, and discrimination in honey bees, a useful model because of their robust olfactory learning and experimentally accessible neural systems. Bees were differentially conditioned with appetitive reward (sucrose) during hexanol exposure and aversive stimulus (quinine) during decanol exposure. To assess olfactory processing and state dependency, …

Developing A Qpcr Method For Evaluating Parasitoid Wasps As A Potential Means Of Control Of The Southern Cattle Fever Tick Rhipicephalus (Boophilus) Microplus In South Texas, Abbey Abalos

Theses and Dissertations

Ixodiphagus spp. wasps are natural enemies of ticks that have been reported to cause mortality in multiple tick species. Parasitoid wasps may represent a potential biocontrol alternative to traditional chemical tick control methods, which are challenged by non-target toxicity and the development of pesticide resistance in species of concern such as Rhipicephalus microplus, a pest of livestock that causes billions of dollars in loss to the livestock industry worldwide. Rhipicephalus sanguineus s.l. is a congeneric species of R. microplus, and parasitism of R. sanguineus by Ixodiphagus sp. in areas of co-occurrence has been observed. It is unknown if R. microplus …

The Impact Of Nutrition On Insecticide Resistance And Gene Expression In Aedes Aegypti, Esmeralda Guadalupe Rosas

Theses and Dissertations

The development of insecticide resistance in Aedes aegypti, a major vector of dengue and Zika viruses, threatens vector control efforts, particularly in endemic regions such as South Texas. Insecticides remain widely used; however, their overuse has accelerated metabolic resistance mediated by cytochrome P450 enzymes. Environmental factors such as larval nutrition may influence mosquito physiology and resistance-associated gene expression, yet this relationship remains poorly understood. This study examines how larval nutritional conditions affect the expression of cytochrome P450 genes linked to pyrethroid resistance in Aedes aegypti. Larvae were reared under five dietary treatments differing in lipid and protein ratios, and adults …

Investigating The Ability Of Foxo1 To Maintain Stem Cells, Stella Aime Rios

Theses and Dissertations

Forkhead box O (Foxo) transcription factors are key regulators of stem cell identity and tumor aggressiveness. Prior work from our group and others demonstrates that Foxo factors maintain stem-like states across diverse biological systems; however, the mechanisms by which Foxo1 suppresses differentiation remain poorly defined.

Using C2C12 myoblasts as a model, we investigated whether Foxo1 preserves stem-like programs through regulation of the nuclear pore protein Nup210. RNA interference was used to generate Foxo1 knockdown cells, followed by qRT-PCR, RNA-sequencing, and fluorescence microscopy. Transcriptomic analysis revealed widespread gene expression changes involving metabolic, mitochondrial, and transcriptional pathways. Notably, Nup210 expression was significantly …

Identification Of Peptides For A Muc13 Cancer Vaccine, Abigail Olivia Gomez

Theses and Dissertations

Mucin 13 (MUC13) is a transmembrane glycoprotein that is frequently overexpressed in epithelial cancers. Its extracellular domains and cytoplasmic signaling potential contribute to disrupted cell adhesion, increased invasion, and cancer-promoting pathways, making MUC13 a promising target for immunotherapy. This project used an in-silico approach to identify MUC13-derived peptides predicted to bind HLA class I molecules and support CD8+ T cell-mediated immune responses. Bioinformatics tools were used to screen candidate epitopes for HLA binding affinity, antigenicity, toxicity, IFN-γ-inducing potential, and population coverage, allowing rational prioritization of peptide candidates for experimentation. Selected peptides were evaluated in THP-1-derived macrophages using RT-PCR, Western blotting, …

Linking North American Demographic Trends To Mexican Non-Breeding Grounds In A Migratory Warbler, Majuwana Gamage Dulanthi Dileka Kariyawasam

Theses and Dissertations

Migration enables species to exploit seasonal resources despite high energetic costs and mortality risks, making it a key focus for conservation under environmental change. I investigated migratory connectivity in Orange-crowned Warblers using stable hydrogen isotopes (δ²H) from feathers collected at nine non-breeding sites across Mexico. Results indicated generally weak connectivity (MC = 0.18), with most sites hosting individuals from six breeding clusters across Canada and the United States. However, one northwestern breeding group showed strong connectivity, suggesting crosswise migration to eastern Mexico. By integrating these findings with long-term Breeding Bird Survey data, I detected contrasting population trends, with Pacific Coast …

Resource Allocation Trade-Offs Between Longevity And Reproduction In Daphnia Magna, Amelia Pearson

Electronic Theses and Dissertations

Although trade-offs between longevity and reproduction have been described for a long time, the genetic mechanisms by which organisms modulate allocation of resources between these two essential processes remain poorly understood, due to their complex nature and their modification by existing nutritional states and environmental cues. Daphnia magna represents a valuable model for investigation in this area due to their moderate lifespan and cyclic parthenogenetic mode of reproduction, allowing for the easy study of gene by environment interactions. This dissertation first investigates the change in mortality, reproduction, activity, and gene expression when fully fed Daphnia and calorically restricted Daphnia are …

Searching Harder For The Darter: Evaluating Endangered Yellowcheek Darter (Nothonotus Moorei) Status And Drought Responses, Kearstin Findley

Graduate Theses and Dissertations

Freshwater ecosystems are one of the most imperiled habitats across the world with biodiversity declining rapidly. There are many stressors contributing to freshwater species declines including habitat loss and degradation, land-use changes, anthropogenic water demands, changes in precipitation and weather extremes, and pollution. Current environmental projections predict that these stressors will continue to impact freshwater ecosystems to greater extents moving forward. Stream drying from drought conditions increasing in intensity and frequency is especially concerning in northern Arkansas where seasonal drought is typical in the summer and fall months. With increased drying conditions, the elimination of shallow-water riffle habitats in streams …

Adventitial Macrophage Accumulation Impairs Perivascular Nerve Function In Mesenteric Arteries With Inflammatory Bowel Disease, Elizabeth A. Grunz, Benjamin W. Jones, Olubodun Michael Lateef, Sidharth Sen, Katie Wilkinson, Trupti Joshi, Erika M. Boerman

Biomedical Sciences

Introduction: Inflammatory bowel disease involves aberrant immune responses and is associated with both cardiovascular disease risk and altered intestinal blood flow. However, little is known about how inflammatory bowel disease affects regulation of perivascular nerves that mediate blood flow. Previous work found perivascular nerve function is impaired in mesenteric arteries with Inflammatory bowel disease. The purpose of this study was to determine the mechanism of impaired perivascular nerve function.

Methods: RNA sequencing was performed on mesenteric arteries from IL10−/− mice treated with H. hepaticus to induce disease (inflammatory bowel disease) or left non-gavaged (Control). For all other studies, Control and …

Tips For Health Care Practitioners: A Guide For Adolescent Obesity, Regina Santana

Electronic Theses, Projects, and Dissertations

Adolescent obesity continues to be a significant public health concern that is associated with long term physical consequences. The purpose of this project was to develop an evidence based, user friendly guidebook that supports healthcare professionals in the education of adolescents with obesity and their families. A structured literature review development process was used to inform the current development of the guidebook. The guidebook includes components of obesity management such as nutrition and physical activity recommendations. The validity of the content was established through the evaluation of five healthcare professionals that include registered dietitians and licensed therapists. The guidebook was …