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Full-Text Articles in Entire DC Network
The Effects Of Heat Stress On White Blood Cell Profiles And Antibody Concentrations In Broilers Genetically Selected For Low Water Conversion Ratio Compared To The Modern Random Bred Broiler, Luke B. Janski
Poultry Science Undergraduate Honors Theses
Heat stress (HS) is one of the most significant environmental stressors impacting poultry production worldwide, reducing growth, feed efficiency, welfare, and immune competence. With global temperatures rising, understanding physiological and immunological adaptations to HS is essential for sustainable production. Broiler chickens selectively bred for low water conversion ratio (LWCR) have shown potential for reduced water consumption under environmental challenges; however, their immune resilience under HS conditions compared with modern random-bred (MRB) broilers remains unclear. This study investigated the effects of cyclic HS on red- (RBC) and white- (WBC) blood cells and humoral immunity in LWCR and MRB broilers. Birds were …
Sequence Determinants Of Rna G-Quadruplex Unfolding By Arg-Rich Regions, Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui-Ting Lee, Jun Zhang
Sequence Determinants Of Rna G-Quadruplex Unfolding By Arg-Rich Regions, Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui-Ting Lee, Jun Zhang
Faculty Publications
RNA sequences with the potential to form G-quadruplexes (rGQs) are widespread but largely unfolded in cells by unknown mechanisms. rGQ folding status is a critical regulator of RNA splicing and translation. We show that rGQs can be unfolded by SR proteins, SR-related proteins, and other Arg-rich proteins, including SRSF1, SRSF3, SRSF9, U1-70K, and U2AF1. The length and composition of Arg-rich regions are key determinants of this activity: Arg residues are the primary drivers, while acidic residues attenuate the unfolding activity. To unfold ARPC2 rGQ, at least 13 Arg residues are required. Our findings identify Arg-rich proteins as previously unrecognized, helicase-independent …
A Series Of Investigations On The L. Cornutus Host System And Bivesiculid Sp. Cercariae, Briana K. Zaffiro
A Series Of Investigations On The L. Cornutus Host System And Bivesiculid Sp. Cercariae, Briana K. Zaffiro
All HCAS Student Capstones, Theses, and Dissertations
Rising global temperatures are likely modulating parasite-host interactions, yet the direction and magnitude of these changes remain uncertain. Rarely are these interactions viewed from both a host and parasite perspective. Using both CTmax, or Critical Thermal Maximum, and Clark-type polarographic oxygen microelectrodes, the physiological metabolic effects are investigated. CTmax is examined in Common Shiners, Luxilis cornutus, and is shown to be modulated by parasitism: uninfected fish had the highest CTmax average of 37.0°C, whereas heavily infected fish had decreased CTmax averaging 35°C. Prior to the parasite metabolic investigation, a suitable medium was determined, as …
Environmental Enrichment Preferences And Play Behavior In Commercial Turkey Hens, Paul Dang
Environmental Enrichment Preferences And Play Behavior In Commercial Turkey Hens, Paul Dang
Poultry Science Undergraduate Honors Theses
Commercial turkey production contributes significantly to the U.S. agricultural industry, yet studies on positive welfare indicators, especially play behavior, are limited. This study evaluated turkey hen interactions with two environmental enrichments (straw bales and pecking stones hung from the ceiling) and the expression of play behavior in turkey barns. Three commercial turkey hen barns were equipped with environmental enrichments and time-lapse video cameras. Play behaviors were recorded during transect walks in two barns using 30-second scans of disturbed and undisturbed patches. Regional use of space around enrichments was quantified through region-of-interest (ROI) analysis of time-lapse images collected from days 16-31 …
Transcriptomic Responses Of Teleost Fishes To Thermal And Environmental Stress: A Synthesis Of Rna-Seq Studies, Aleen Ammar
Transcriptomic Responses Of Teleost Fishes To Thermal And Environmental Stress: A Synthesis Of Rna-Seq Studies, Aleen Ammar
Theses, Dissertations and Culminating Projects
Climate change is driving rapid shifts in aquatic ecosystems, increasing the frequency and intensity of environmental stressors that affect survival, species distributions, and productivity. Alongside increases in temperature, stressors, such as hypoxia and acidification, are also intensifying with climate change. Teleost fishes exhibit substantial variation in tolerance to such environmental stressors. These range from specialists with narrow limits to generalists capable of persisting across fluctuating conditions, complicating predictions of vulnerability under ongoing environmental change. Transcriptomic approaches, particularly RNA sequencing (RNA-seq), provide a powerful approach for linking environmental stressors to physiological responses by quantifying genome-wide changes in gene expression. To assess …
Phenological Shifts In Reproductive Timing Of Bay Scallops (Argopecten Irradians) In New Jersey Following Subspecies Replacement, Ross John Choate Jr.
Phenological Shifts In Reproductive Timing Of Bay Scallops (Argopecten Irradians) In New Jersey Following Subspecies Replacement, Ross John Choate Jr.
Theses, Dissertations and Culminating Projects
Bay scallops (Argopecten irradians) are commercially, ecologically, and recreationally important bivalves that have undergone major shifts in New Jersey over the past 25 years, including the extirpation of the southern subspecies from New Jersey in 2001 and its replacement by the northern subspecies from Long Island by 2004. This transition altered reproductive timing, reflected in changes to the Gonadal–Somatic Index (GSI) as well as the timing of visual gonadal conditions exhibited during specific months. Early post-reestablishment data showed peak GSI in June and July, consistent with Long Island populations, but recent observations indicate a shift toward August and September peaks, …
Assessing Decadal Changes In Eelgrass Communities From Barnegat Bay, Nj, Janelle Marie Delcastillo
Assessing Decadal Changes In Eelgrass Communities From Barnegat Bay, Nj, Janelle Marie Delcastillo
Theses, Dissertations and Culminating Projects
Zostera marina (eelgrass) is a widely distributed marine angiosperm across northern hemisphere coastlines, yet many regions have experienced substantial declines due to disease, coastal development, and other anthropogenic stressors. Barnegat Bay is the only estuary in New Jersey that still supports extensive eelgrass beds, although it has undergone notable losses over the past five decades. To evaluate decadal-scale ecological change, we collected sediment cores in 2025 from a site with historical data spanning 2001–2010. Overall faunal densities remained relatively consistent, but several taxa exhibited clear shifts in abundance. Amphipods in the Phoxocephalidae and the isopod Erichsonella increased, whereas the cold-affinity …
Historic Patterns Of Growth And Destruction In Nj Seagrass Meadows, Masen Eck
Historic Patterns Of Growth And Destruction In Nj Seagrass Meadows, Masen Eck
Theses, Dissertations and Culminating Projects
Seagrass meadows are one of the many habitats worldwide under threat from human disturbance and worsening climate conditions. This habitat provides many critical ecosystem services to coastal environments, which is why conserving and restoring seagrass meadows is critical. This study investigates the long-term success and failures from several eelgrass restoration sites in Barnegat Bay, New Jersey. Aerial imagery from multiple years were digitized in ArcGIS to assess restoration trajectories over time. Three of the sites were assessed over a period of twelve years (2012-2023), and one site was assessed twice (2021 and 2023). Results from these time series analyses showed …
Shape Variation In The Mandible Of The Borophagine Canids And Implications For Predatory Behavior, Benjamin Neavear
Shape Variation In The Mandible Of The Borophagine Canids And Implications For Predatory Behavior, Benjamin Neavear
Theses, Dissertations and Culminating Projects
The borophagines are an extinct lineage of canids that inhabited North America for more than 20 million years. This group included genera such as Aelurodon, Borophagus, and Epicyon. Borophagines are commonly referred to as “bone-crushing dogs” because their skull morphology converged with that of extant hyenas, predators capable of consuming bone. The purpose of this study was to determine whether convergence was present in the mandible, an anatomical structure strongly correlated with feeding behavior, particularly in the genera Aelurodon and Borophagus. It was hypothesized that most borophagine taxa possessed mandibular shapes similar to those of hyenas. Numerous mandibular characters were …
Evaluation Of Flt3 Inhibition With Quizartinib During Busulfan Conditioning For Hematopoietic Stem Cell Transplantation, Catherine Jankovic
Evaluation Of Flt3 Inhibition With Quizartinib During Busulfan Conditioning For Hematopoietic Stem Cell Transplantation, Catherine Jankovic
Biochemistry and Molecular Biology
Hematopoietic stem cell transplantation (HSCT) is one of the only curative therapy options for many hematologic malignancies. HSCT relies on effective conditioning regimens to deplete host hematopoietic cells and enable donor engraftment. The alkylating agent Busulfan is widely used in myeloablative conditioning strategies due to its potent cytotoxic effects on hematopoietic cells and ability to clear the bone marrow. However, optimization of conditioning strategies remains an important area of investigation to improve transplant outcomes and reduce toxicity. Preliminary studies have shown that the FLT3 inhibitor Quizartinib synergizes with some chemotherapeutic agents, suggesting that targeted inhibitors may enhance conventional conditioning regimens. …
Structural Evidence For Innervation Of A Single Epitheliomuscular Cell By Multiple Sensory Neurons In The Sea Anemone Nematostella Vectensis, Aparajita Das
Graduate Theses and Dissertations
Abstract Here, I report new ultrastructural evidence for multineuronal innervation (many-one) of a single epithelia-muscular cell in sea-anemone cnidarian Nematostella vectensis. Using Serial Block-Face Scanning Electron Microscopy (SBF-SEM) and ImageJ, I found that EMCs are located at the base of ectoderm, with an elongated cell body that includes basal processes, a bilobed nucleus, and myofibrils aligned parallel to the basal surface along with their structural quantification. Notably, I found multiple mechanosensory neurons form synapses with a single EMC. These findings enhance our understanding of cnidarian neuromuscular organization and reveal that coordination between sensory and contractile systems in N. vectensis is …
Beat Behind The Bark: Investigating The Effects Of Music Tempo On Canine Physiological And Psychological States, Marley Lanier
Beat Behind The Bark: Investigating The Effects Of Music Tempo On Canine Physiological And Psychological States, Marley Lanier
Honors Theses
This study explores how the tempo of music, measured in beats per minute (BPM), affects dogs' heart rate and oxytocin levels, adding to past research that shows that music affects physiological and behavioral states in dogs. Being able to understand these effects holds practical implications for improving overall well-being in canines in environments such as homes, shelters, and clinical environments. Using a within-subjects design, the dogs were exposed to slow and fast versions of one instrumental track, along with a white noise baseline condition before each randomized condition. Heart rate was recorded continuously throughout the session, and oxytocin saliva samples …
Bottles, Butterflies, And Biomass: Recycled Glass Sand, Microbial Additions, And Herbivory Effects On Milkweed Growth, Kealey Skinner
Bottles, Butterflies, And Biomass: Recycled Glass Sand, Microbial Additions, And Herbivory Effects On Milkweed Growth, Kealey Skinner
Honors Theses
Plant-soil-microbe interactions are integral components of ecological networks and drive ecosystem function from the bottom-up. Whereas herbivores regulate ecosystem function from the top-down, having opposing and potentially complementary effects on ecosystem function. My thesis sought to understand how below-ground microbial communities contribute to herbivore preference and plant biomass across varied soil substrates. My thesis asked: how do substrate and soil microbial communities influence herbivore preference and plant biomass accumulation on swamp milkweed (Asclepias incarnata)? To address this question, seedlings of A. incarnata were sown in 2.5 cm wide × 10 cm deep pots filled with either natural beach sand or …
Distribution Mapping And Modeling Of The Invasive Japanese Knotweed, Jossalyn Rogalski
Distribution Mapping And Modeling Of The Invasive Japanese Knotweed, Jossalyn Rogalski
Masters Theses
This thesis investigates the distribution of invasive Japanese knotweed across Michigan. This plant poses significant challenges to land management due to its rapid growth, environmental impacts, and difficulty of removal once established. Using geographic information systems (GIS), remotely sensed data, and secondary environmental datasets, this research maps current infestations and analyzes key factors influencing the species’ growth and dispersal, such as soil composition, hydrology, land use, and a range of environmental variables, including climatic (annual mean temperature, temperature seasonality, annual precipitation, precipitation seasonality), elevation, vegetation and hydrological indices (NDVI, EVI, NDWI), percent tree cover, and distance to roads. Spatial analysis …
Evaluating The Impact Of Intercropping Vegetables And Cut Flowers On Yield And Conservation Biological Control, Alliance Igiraneza
Evaluating The Impact Of Intercropping Vegetables And Cut Flowers On Yield And Conservation Biological Control, Alliance Igiraneza
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
Specialty crops such as vegetables are some of the most susceptible to yield losses and increased management difficulty over time due to pests. One of the potential solutions to this problem, which aims at ensuring the sustainability of the sector, is using conservation biological control practices such as intercropping to increase diversity, while maintaining the economic feasibility and profitability of the system. This study was conducted to evaluate the impact of this management practice using Bell pepper (Capsicum annuum var. King Arthur) as the main crop, intercropped with flowers including zinnia (Zinnia elegans var. Benary’s Giant), sunflower ( …
Herbivory Of Phragmites Australis Along A Salinity Gradient In Connecticut Wetlands, Sydney H. Kolz
Herbivory Of Phragmites Australis Along A Salinity Gradient In Connecticut Wetlands, Sydney H. Kolz
Honors Scholar Theses
Invasive Phragmites australis (common reed) is widely distributed in New England wetlands, where it often outcompetes native plants and decreases biodiversity throughout the food web. Controlling its invasion is a priority, especially in salt marsh habitat, but current management techniques are inefficient. Leveraging natural processes may improve control strategies, but in order to do so, we must first better understand how abiotic and biotic stressors interact and affect Phragmites. This study focuses on herbivory and salinity, two factors limiting Phragmites’ fitness. I measured soil salinity and quantified the damage of three herbivore guilds (i.e. gall-formers and stem-borers, leaf-chewers, and …
Influence Of Increased Supplementation Of Organic-Complexed Zinc To Beef Cows During Gestation: Effects On Preweaning Health Of Offspring, Naija N. Sharpley
Influence Of Increased Supplementation Of Organic-Complexed Zinc To Beef Cows During Gestation: Effects On Preweaning Health Of Offspring, Naija N. Sharpley
Animal Science Undergraduate Honors Theses
The objective of this study was to determine the effect of increasing zinc supplementation from organic-complexed sources to beef cows during gestation on calf performance. In May 2025, during gestation, Angus crossbred cows were confirmed pregnant with female sexed semen (n = 83) and enrolled in the current study. Cows (4.7 ± 2 years of age and 531 ± 84.4 kg body weight, mean ± standard deviation) were allotted to 4 groups (20 to 21 cows/group) based on, sire of heifer calf, cow age, and cow body weights taken at weaning. Assigned groups received 1 of 2 treatments (2 groups/treatment): …
Identifying The Role Of Human Dna Polymerase Delta During Human Cytomegalovirus Genome Synthesis, Rachel Laree Dansereau
Identifying The Role Of Human Dna Polymerase Delta During Human Cytomegalovirus Genome Synthesis, Rachel Laree Dansereau
Dartmouth College Master’s Theses
Human cytomegalovirus (HCMV) is a large double stranded DNA virus and member of the Herpesviridae family. Like all herpesviruses, HCMV presents with characteristics of persistent, lifelong infections that transition between lytic and latent states. The replication of the viral genome is traditionally thought to occur by a rolling circle mechanism, but this model is supported by little evidence. It is known that HCMV actively recruits several host proteins to viral replication compartments (RCs), including proteins involved in the DNA damage response (DDR). The exact mechanisms of how these proteins are used and in what ways they help the virus remain …
Using The Flexon Approach To Rnai For Analysis Of Gene Function In Gonadal Development In C. Elegans, Andrew D. Spalding
Using The Flexon Approach To Rnai For Analysis Of Gene Function In Gonadal Development In C. Elegans, Andrew D. Spalding
Undergraduate Theses
C. elegans, a nematode worm, was used as a model organism to analyze the developmental effects of knocking down the expression of genes that are both essential to worm viability and highly enriched in reproductive tissues. In earlier research, approximately 70 C. elegans genes were identified that are both gonad-enriched and essential. To prioritize which genes should be the focus of future studies, a new strain of C. elegans was produced with three specific genetic variations that: 1) silence essential, somatic gonad-enriched gene expression, 2) limit the effects of this gene silencing to the gonad, and 3) yield a …
Using A Toxic Aging Coin To Assess Hexavalent Chromium Neurotoxicity: Impact Of Cr(Vi) On The Blood-Brain Barrier, Liam Buchanan
Using A Toxic Aging Coin To Assess Hexavalent Chromium Neurotoxicity: Impact Of Cr(Vi) On The Blood-Brain Barrier, Liam Buchanan
College of Arts & Sciences Senior Theses
The US population faces simultaneous public health threats from both aging and environmental pollutants. Chief among these is hexavalent chromium [Cr(VI)], whose toxic effects in peripheral tissues are well-established but its effects on the brain are still understudied. Following previous results indicating hippocampal metals dyshomeostasis after Cr(VI) exposure, we aim to assess Cr(VI)’s impact on the blood-brain barrier (BBB), a key structure for brain protection. We exposed young, middle-aged, and geriatric rats of both sexes to low levels of Cr(VI) in their drinking water for 90 days, after which we used immunofluorescence and confocal microscopy to analyze tissues for evidence …
Cholesterol Effect On The P-Glycoprotein Conformational Dynamics, Zane Bunn
Cholesterol Effect On The P-Glycoprotein Conformational Dynamics, Zane Bunn
Biological Sciences Undergraduate Honors Theses
P-Glycoprotein (Pgp) is a human efflux pump that belongs to the ABC transporter family. This protein consists of two transmembrane domains (TMD) that convert between inward to outward facing configurations to remove a wide variety of structurally different compounds from the intracellular membrane into the extracellular space using ATP. This non-specific efflux of compounds has brought Pgp to scientific light as having a major role in multi-drug resistance of many diseases, and researchers are focused on developing a deeper understanding of the protein. To further this understanding, this study analyzed how the structure of Pgp is affected by a 10% …
Hormonal, Cytokinetic, And Skeletal Response To A Single Weighted Vest Exercise Treatment: A Pilot Study To Determine Weighted Vest “Rucking” Treatment For Osteoporosis In Post-Menopausal Women, Eleanor Pell
Master's Theses
Osteoporosis is characterized by a deteriorating of bone tissue leading to brittleness and increased fracture risk that affects both men and women as they age. Postmenopausal women are of particular interest due to high risk of declining bone density, a characteristic of this population. Treatments for osteoporosis range from menopause hormone therapy, bisphosphonates, biologics, nutritional changes, and exercise. Load bearing exercise interventions have shown effectiveness for stimulating bone growth, but many factors such as lack of access, motivation, self-confidence or knowledge, prevent long term compliance. “Weighted vest rucking” refers to a variation of rucking, which is a form of exercise …
Companion Parrot Vocal Learning And Reproduction Of Human Speech Sounds, Karla Vilches Castaño
Companion Parrot Vocal Learning And Reproduction Of Human Speech Sounds, Karla Vilches Castaño
Master's Theses
Vocal learning is rare among animals but is well developed in parrots, which can imitate novel sounds and, in captive settings, reproduce human speech. This study helps address a larger evolutionary question about vocal learning. Specifically, whether vocal learning ability is best understood as a single generalized trait or as a multi-dimensional system in which different acoustic features can be learned and reproduced with varying degrees of fidelity. This thesis quantifies how closely companion parrots match their human tutors across three acoustic dimensions (spectrographic structure, intensity contour, and pitch contour) and tests whether mimicry accuracy varies among individuals, differs between …
Investigating The Taxonomic Uncertainties Of Potentilla Rupincola, Natalie Elizabeth Hieber
Investigating The Taxonomic Uncertainties Of Potentilla Rupincola, Natalie Elizabeth Hieber
Master's Theses
Landscape stewardship is an integral role for land managers that becomes more important when rare or endangered species occur within managed areas. Conservation across the landscape is a primary concern for land managers, but resources allocated for conservation can be limited due to many competing goals. Taxonomic uncertainty surrounding rare and endangered taxa, particularly plant taxa, that have historically been classified solely using morphological features further complicates conservation planning. One such taxon is Potentilla rupincola, a rare plant endemic to the eastern Rocky Mountains in Colorado. For over a century, botanists have debated whether P. rupincola is a distinct species …
The Effects Of Impervious Land Cover And Red Tide (Karenia Brevis) On The Florida Manatee (Trichechus Manatus Latirostris) In The Gulf Coast Of Florida, Anna Compton
Honors College Theses
Red tide, caused by the dinoflagellate Karenia brevis, is an algal bloom event that occurs annually in the West Florida Continental Shelf. The algae releases brevetoxin, a toxin that can be fatal to wildlife in high concentrations. These algal bloom events are thought to be exacerbated by warming ocean temperatures and increased nutrient runoff from urbanization. Red tide is responsible for the mass mortality of various marine organisms like the Florida manatee (Trichechus manatus latirostris). This study analyzes the changes in nutrient runoff and sea surface temperatures, and how these factors affected red tide cell concentrations and Florida manatee mortalities …
2026 Workshop On Bridging The Gaps: Tackling Microplastics And Nanoplastics Challenges In Coastal Ecosystems, Shenghua Wu, Kaushik Venkiteshwaran, Melike Dizbay-Onat, Alexandra Stenson, Jinhui Wang, Tina Miller-Way, Ebenezer Nyadjro, Bhuvnesh Bharti, John Weinstein, Yi Bao, Shihui Shen, Valerie Longa, Shenghua Zha, John Cleary, Marty Lind
2026 Workshop On Bridging The Gaps: Tackling Microplastics And Nanoplastics Challenges In Coastal Ecosystems, Shenghua Wu, Kaushik Venkiteshwaran, Melike Dizbay-Onat, Alexandra Stenson, Jinhui Wang, Tina Miller-Way, Ebenezer Nyadjro, Bhuvnesh Bharti, John Weinstein, Yi Bao, Shihui Shen, Valerie Longa, Shenghua Zha, John Cleary, Marty Lind
Workshops
This report presents the findings from the 2026 Workshop on Tackling Microplastics and Nanoplastics (MNPs) in Coastal Ecosystems, which took place in Mobile, Alabama. More than 140 experts from universities, government, industry, and community organizations gathered to address the increasing issue of MNPs in coastal regions.
Sorghum Grain Secondary Metabolites And Underlying Genetics To Reduce Foodborne Pathogens In Poultry, Maria A. Conti
Sorghum Grain Secondary Metabolites And Underlying Genetics To Reduce Foodborne Pathogens In Poultry, Maria A. Conti
All Dissertations
The rise in antibiotic-resistant pathogens poses a threat to the poultry industry's ability to maintain animal health, prompting growing interest in alternatives to synthetic antibiotics. The use of non-tannin sorghum [Sorghum bicolor (L.) Moench] in poultry rations offers a valuable alternative, as this cereal grain has a high bioactive profile that can provide health benefits, including antimicrobial (AM) activity. The scope of this study was to examine the AM potential of non-tannin sorghum grain against Clostridium perfringens, a major foodborne pathogen that negatively impacts poultry health and production. To that end, the inhibitory effects of a non-tannin sorghum …
Developing Warning Signage For Harmful Algal Blooms At Lake Fayetteville, Abigail R. Evans
Developing Warning Signage For Harmful Algal Blooms At Lake Fayetteville, Abigail R. Evans
Biological Sciences Undergraduate Honors Theses
The presence of harmful algal blooms (HABs) poses many risk factors for human health, and once microcystin concentration has reached a certain level, that water source will be closed for the duration of the bloom. Agencies tend to use static signage with limited information to inform the public about the current status of the water, but a sign similar to the one used by the National Fire Danger Rating System can allow for constant updates detailing specific risk levels for the public. Using previously reported data on Lake Fayetteville, thresholds for risk factors that are easily measurable were determined. The …
The Genetic Basis Of High Transformation Efficiency In The Model Yeast Saccharomyces Cerevisiae, Mary Kathryn E. Cheek
The Genetic Basis Of High Transformation Efficiency In The Model Yeast Saccharomyces Cerevisiae, Mary Kathryn E. Cheek
Biological Sciences Undergraduate Honors Theses
Transformation, a type of horizontal gene transfer, describes the uptake, maintenance, and integration of environmental DNA by cells. Despite the known effects of transformation on evolutionary processes, little is known about the genetic basis of this phenomenon. Saccharomyces cerevisiae is a eukaryote able to transform at quantifiable rates when made chemically competent and treated with a heat shock. S. cerevisiae also has variation in transformation efficiencies between various strains, and high recombination rates allow for sufficient allele “shuffling” in intercross populations. This project utilizes a bulk segregant analysis approach to compare sequencing data between transformant pools and non-transformant pools of …
Analysis Of Developmental Cardiogenic Processes Mediated By Fox And Zinc-Finger Transcription Factors, Rajnandani Katariya
Analysis Of Developmental Cardiogenic Processes Mediated By Fox And Zinc-Finger Transcription Factors, Rajnandani Katariya
All-Inclusive List of Electronic Theses and Dissertations
Cardiogenesis is a highly choreographed biological process requiring the precise integration of gene regulatory networks to govern cell division, fate determination, and spatial positioning. Disruptions in these mechanisms lead to congenital heart defects (CHDs), which affect approximately 1% of live births globally. Due to the evolutionary conservation of cardiac signaling pathways and the structural homologies between the Drosophila dorsal vessel and the early vertebrate heart tube, Drosophila melanogaster serves as a powerful model for dissecting the fundamental mechanisms of heart development. This dissertation investigates the molecular orchestration of cardiac development, specifically focusing on the roles of the transcription factors Jumu …