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Partial Purification & Kinetic Studies Of Nicotinamide Adenine Dinucleotide Phosphate-Specific Isocitrate Dehydrogenase Of Phycomyces Blakesleeanus, Michael Meredith

Masters Theses & Specialist Projects

The nicotinamide adenine dinucleotide phosphate -specific isocitrate dehydrogenase (threo-Ds(+) isocitrate: NADP⁺: oxidoreductase [decarboxylating]; E.C. 1.1.1.42.) of Phycomyces blakesleeanus was partially purified. The method used to purify the enzyme was a combination of protamine sulphate precipitation, ammonium sulphate fractionation, and ion exchange and gel filtration chromatography. The NADP⁺ -specific IDH was purified from an initial specific activity of 0.05 units/mg of protein to a final specific activity of 1.5 units/mg protein in solution.

Molecular and reaction characteristics were explored. Employing gel filtration the molecular weight of the enzyme was determined to be 88,000. The forward reaction was found …

The Fresh-Water Mussel Industry Of The Lower Tennessee River: Ecology & Future, Randall Grace

Masters Theses & Specialist Projects

The fresh-water mussel industry of the Tennessee River is nearing an end. Overharvesting, habitat alterations, and pollution are the major contributors to the depletion of the mussel resource, upon which the shell industry is based. A history of unconcern by shell harvesters and weak conservation enforcement by governmental agencies, has left the major waterways of the United States nearly void of commercial clams. The lower Tennessee River presently supplies the mussel industry with nearly all the important species of mollusks. If this industry is to be maintained in the United States, ways to preserve and propagate the mussel population must …

Kentucky Warbler (Vol. 50, No. 2), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Seed Reserves In Desert Soils, David W. Goodall, Suzanne J. Morgan

Memorandum

Soil and litter samples were collected from the playa, playa fringe and bajada zones of the Jornada Validation Site. These samples were analyzed for their seed content, special attention being paid to spatial distribution. At the time of sampling, there was little litter on the soil surface, and the litter contained only a small proportion of the total seeds present.

The Effects Of Temperature & Photoperiod On Molting In Seasonal Populations Of The Crayfish Orconectes Rusticus Rusticus (Girard), Steven Sadewasser

Masters Theses & Specialist Projects

Immature Orconectes rusticus rusticus (Girard) were collected from Doe Run, Meade Co., Ky. on January 16, June 1, and October 21, 1972, for the purpose of examining the effects of three different temperatures (14C, 18C, and 22C) and three different photo, eriods (6L:18D, 15L:9D, and 24LL) on seasonal molting patterns. For each seasonal experiment, 144 crayfish were placed in 3 x 3 experimental units and maintained for a 90-day period.

In Experiment 1 (January 18-April 18), 98 molts were attempted with 98% of the molts being successfully completed. Arrangement of these molts within the separate treatments of this experiment indicated …

Lesion Formation In The Liver Of Mice Caused By Metabolic By-Products Of Hymenolepsis Microstoma, Gary Simpson

Masters Theses & Specialist Projects

The presence of lesions in the liver of mice is associated with the infection of these mice with the common bile duct -dwelling cestode, Hymenolepis microstoma. Lesions develop even though there is no direct contact between the cestodes and the liver. It was found in this investigation that administration to mice of Tyrode's solution, in which cestodes had been incubated for 24 hr, resulted in liver lesions comparable to those observed in normal infections. The administration of the causative substance by either per os intubation or intraperitoneal injection produced similar lesions. Further studies revealed that the active lesion - …

Canalization Of Scutellar Bristles In Pyd And Pyd H Populations Of Drosophila Melanogaster, Rita Kathleen Bryson

Biology ETDs

No abstract provided.

The Effects Of The Toxins Of Lophopodella Carteri (Ectoprocta) On The Blood Gas Properties Of The Bluegill Lepomis Macrochirus, Robert Allen May

Master's Theses

Lepomis macrochirus (bluegill) were exposed, in small containers, to homogenates of Lophopodella carteri, an ectoproct that contains substances toxic to gilled vertebrates. Blood pH, pco₂, and po₂ were determined with a blood gas analyzer. Blood carbonic anhydrase activity was measured manometrically.

The ectoproct toxins caused significant decreases in blood pH and po₂ and a significant increase in blood pco₂ and carbonic anhydrase activity. It is proposed that these changes in blood properties were initiated by a film of mucus covering the gill epithelium. The secretion of mucus is thought to be a response to irritation …

Histologic Comparison Of Apical Tissue Response To Endodontic Overfills With Gutta Percha And Grossman's Sealer Vs. Sargenti Method (N₂), Donald L. Brewer

Loma Linda University Electronic Theses, Dissertations & Projects

This study compared endodontic overfills of N₂ and gutta percha-Grossman's Sealer from a histopathological basis. The study employed four male rhesus monkeys and a sample of 31 teeth.

The most obvious difference between the samples filled with N₂ and the gutta percha-Grossman's Sealer samples was the recurring presence of ankylosis in the N₂-treated teeth. Results also included bone necrosis, inflammation and root and bone resorption.

The N₂ samples were considered to have evidenced greater violence of reaction apically.

Seasonal Periodicity Of Dominant, Benthic, Marine Macroalgae Of The North Jetty Of Brazos Santiago Pass, Texas, As Related To Environmental Factors, Gary J. Penn

Theses and Dissertations - UTB/UTPA

The seasonality of marine algae may be influenced by a large number of factors. These factors Include water clarity, water temperature, dissolved oxygen, conductivity, salinity, light Intensity, precipitation, air temperature, water turbulence, and daylength. The purpose of this study was to determine seasonal patterns and the effect of these variables on the algae.

The results Indicate that 24 dominant, benthic, genera of algae have two seasonal populations. A winter-spring population Includes algae in abundance between January and May. A fall population includes algae in abundance during September, October and November, Results of environmental studies indicate day-length dominant factors in algal …

The Effects Of Herbicidal Spraying Upon A North Central Utah Blue Grouse Population, T. Barry Barnes

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effects of herbicidal spraying upon a North Central Utah blue grouse population were studied. Baseline data were obtained in 1970 and 1971 prior to the spraying on June 2, 1972. The spraying was done to control wyethia (Wyethia amplexicaulis) and black sage (Artimesia nova) which covered 48 percent and 20 percent of the study area respectively.

No differences in blue grouse numbers occurred following spraying with 17 to 20 broods using the area in 1972 compared to 18 to 20 broods in 1971. Total population of blue grouse each year was between 90 and 104 …

Rates Of Food Digestion By Brown Trout (Salmo Trutta) In The Blacksmith Fork River, Utah, John M. Salevurakis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Digestion rates have been measured for brown trout (Salmo trutta Linn.) during summer, fall, winter and spring seasons on the Blacksmith Fork River, Utah. Exponential rates of digestion varied from a gradual rate of -0.2372 to an accelerated rate of -0.6808. Factors found to affect digestion rate most were water temperature and the amount of food in the stomach. The affect of temperature was not clearly isolated. However, the amount of food in the stomach, at the beginning of the digestion study, appeared to have the most pronounced effect. Four of the five digestion rate measurements, with high coefficients …

Vocalizations In A Population Of Green-Tailed Towhees (Chlorura Chlorura), Timothy Andrus Burr

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Studies of vocalizations in a montane population of Green-Tailed Towhees were conducted during the summer months of 1971 and 1972 in Cache County, Utah. The song and call repertoiries of 10 breeding pairs were recorded and analyzed on a sound spectograph.

Males averaged 8.7 different song and 18.2 different not types. Song variation on the population level is high (58 different songs) but the sharing of these songs among the males is low (22.4). Populational note structure is equally diverse but reveals a greater degree of sharing (75%).

Both males and females used three calls, the meow, tick, …

Life History And Habitat Utilization Of Merriam's Turkey In Southwestern Utah, Fred C. Bryant

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A study of the Merriam's wild turkey was conducted March 1972 to August 1973 in southwestern Utah to determine the life history and habitat utilization. Using transects, bio-telemetry, and observational techniques, information on flocking, hen:poult and sex ratios, and approximate dates for spring dispersal, nesting, and hatching was obtained. Turkey observations in relation to habitat variables were used to determine habitat utilization.

Except during the spring mating season, male flocks remained apart from other turkeys. Hen flocks in fall and winter consisted of adult hens and juveniles of both sexes. Males dispersed from winter flocks in March, and those that …

Characterization Of Selected Bacteria From The North Arm Of The Great Salt Lake, John L. Crane Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Thirteen bacterial cultures were isolated from the North arm of Great Salt Lake during the months of January and February of 1973. Eleven isolates were gram-negative pleomorphic rods which lysed in hypotonic solution. The remaining two were gram-positive cocci. All isolates and one known strain of Halobacterium salinarium were subjected to examination of morphological, cultural, physiological, and biochemical characteristics. A numerical taxonomic analysis was applied to the compiled characters to compute a coefficient of similarity for each individual isolate as compared to all other isolates. A comparative analysis was included in the similarity computation using characters assembled from those reported …

Biochemical And Physiological Studies On Phytotoxicity Of Selected Pesticides And Allergens During Seed Germination Of Some Food Crops, R. R. Dalvi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Germination of mung bean, Phaseolus mungo L. , and wheat, Triticum aestivum L., seeds was used for bioassay to demonstrate the toxic effects of selected pesticides--menazon, disulfoton, and GS-14254-- and allergens-- alantolactone and usnic acid. The ability of gibberellic acid to counteract the toxic effects of these chemicals on germination and seedling growth was studied. Chemical composition of the treated and untreated seeds was made with special attention to starch and protein degradation. Effect of these toxicants on the synthesis of amylase, ATPase, and protease enzymes during germination was studied since these enzymes are synthesized de novo during germination. To …

Endemic Whitefishes Of Bear Lake, Utah-Idaho: A Problem In Systematics, Robert G. White

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The systematic status of whitefishes endemic to Bear lake, Utah-Idaho, has remained tenuous since their original description. Clarification of this problem was the major objective of the present study. The general approach was an integrated one, including examination of morphological, biochemical and ecological parameters; artificial hybrids were produced and compared with questionable groups from the natural population.

Morphological analysis revealed five forms of Bear Lake whitefishes Prosopium gemmiferum (Bonneville cisco) and P. abyssicola (Bear lake whitefish) were well differentiated from other forms and were treated as originally described. The P. spilonotus (Bonneville whitefish) group, however, was found to be made …

Influence Of Soil Water Potential And Environment On The Internal Water Status Of Grasses, Ray W. Brown

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The water relations of two grass species, smooth brome (Bromus inermis Leyss.) and intermediate wheatgrass (Agropyron intermedium (Host) Beauv.), were studied under a range of environmental conditions in a growth chamber. The environmental conditions included three temperature regimes (day/night temperatures of 15/10, 20/15, and 25/20 degrees centigrade), and three vapor pressure deficits within each temperature regime (6.2, 8.8, and 11.4 millimeters of mercury). Short wave radiation was maintained at 0.5 calories per square centimeter per minute with a 14 hour photoperiod, and wind speed was constant at 45 centimeters per second.

Six plants of each species were studied …

A Study Of The Radiation Quality Under Plant Canopies In The Wave Range 0.4 To 2.5 Microns, Nolasco G. Baldazo

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The spectral distribution of the global radiation from 0.4 to 2.5 microns penetrating deciduous and coniferous canopies were measured during clear days between 10 a.m. and 2 p.m. using a double-quartz monochromator.

In the visible region (0.4 to 0.7 micron) the average relative spectral transmissions under both canopies are about one percent beginning at 0.4 micron and decreasing to about half a percent at 0.67 micron. There is only a small peak in the green (0.55 micron) transmission under deciduous stands while there is none under coniferous canopies. The slightly higher transmission in the blue (0.4 micron) is attributed to …

Nutrition Of Sheep Grazing Foothill Big Game Range In Spring, Kurt J. Kotter

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Sheep with esophageal fistulas were used to determine the daily intake, nutritive content and digestibility of forage at three periods and two stocking intensities during the spring of 1972 on a typical foothill range in northern Utah.

Heavy grazing under a season-long regime did not influence the concentrations of dietary chemical components when compared to moderate grazing; however, it did depress the digestibility of cellulose and organic matter. There was a significant decline in the dietary chemical components due to forage maturation. Digestibility of organic matter and cellulose were significantly higher in the early spring as compared to late spring. …

An Evaluation Of In-Stream Structures Designed To Provide Fish Habitat, Charles H. Call Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In-stream concrete structures were studied through model tests and river tests. The model studies indicated that four designs provided good habitat in the model stream. These structures were the inverted weir, the "V" structure, the slab with legs and the cylinder. Through the river studies it was determined that these structures did not influence enough of the total river area to be effective in providing good fish habitat. Also an appreciable amount of yearly maintenance would be required to free the structures from silting in, debris, and vandalism. The slab with legs was the only promising structure.

The Dynamics Of Root Growth And The Partitioning Of Photosynthates In Cool Desert Shrubs, Osvaldo Alberto Fernandez

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study addresses the nature of physiological and phenological evolutionary strategies of root growth dynamics and energy allocation followed by Atriplex confertifolia, Ceratoides lanata, and Artemisia tridentata growing in their natural cool desert environment.

Root observation chambers with inclined Plexiglass windows were installed in monospecific desert communities of Atriplex confertifolia, Ceratoides lanata and Artemisia tridentata. Soil temperature and water potential measurements taken immediately adjacent to the observation windows indicated a minimal disturbance was caused by the presence of these chambers. For the three species initiation of root growth was observed before initiation of shoot activity, furthermore, active root growth extended …

Economic Feasibility Of Controlling Big Sagebrush (Artemisia Tridentata) On State And Private Rangelands In Utah, Stan D. Hinckley

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Spraying with the chemical herbicide 2,4-D is the most widely used method of controlling big sagebrush. Spraying is very effective in increasing forage production and generally is not poisonous to either man or animals.

Two procedures can be used to calculate the internal rate of return to big sagebrush control: standard and modified discounting. Standard discounting assumes all nonuse costs are incurred in the year of treatment, and the annual income stream is constant throughout the effective life of treatment. Modified discounting correctly assumes the nonuse cost is incurred in the period of deferment, and the income stream does not …

Anatomical Effects Of Dicamba On Pea Root Tissues, Brent George Ovard

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Peas (Pisum sativum L. Var Alaska) were allowed to absorb calcium and magnesium chloride for 8 hours and then were germinated in a potassium phosphate buffer pH 6.5 for 40 hours. Peas were then treated with 0, 0.1, 0.3, or 0.5 ppm dicamba (3,6-dichloro-o-anisic acid) and harvested at 24, 48, and 72 hour intervals. The following determinations were recorded: root elongation, nuclear volume, and anatomical modifications.

Root elongation recordings showed that dicamba prevented normal root elongation. Treatments induced very short thick primary roots.

Measurements of nuclear volume indicated that all herbicide concentrations were able to reduce the …

A Study Concerning Sources Of Information And Knowledge About Fluoride In Logan, Milford, And Helper, Utah, Marilyn Lorraine Geddes

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A considerable amount of research has been conducted concerning the characteristics of people who are in favor of fluoridation. However, there is little information available as to what the electorate really knows about fluoride when they are forced to vote on this scientific issue.

Thus, the objectives of this study were to determine the general public's level of knowledge about fluoride, to discover what sources of information have been the greatest influence on their opinion and knowledge about fluoride, and to estimate the level of participation of dentists, general practitioners, pediatricians, and gynecologists in the education of Utahns about the …

The Phenology And Control Of Dyers Woad (Isatis Tinctoria) In Northern Utah, William A. Varga

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this thesis is to describe dyers woad and to establish v means for control of the weed in northern Utah. Logan was chosen for studying germination of the weed, and interaction of dyers woad and alfalfa stands. Collinston was selected for screening trials of herbicides for the control of dyers woad. The thesis contains information on the phenology of dyers woad and suggestions for control of the weed.

Seasonal Changes Of Peach Flower Buds At The Ultrastructural Level, Yeh Feng

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was conducted to gain a better understanding of the seasonal changes of Gleason Elberta peach flower buds at the cellular level. Peach buds were obtained from the Howell Experiment Station in Ogden, Utah. Samples were collected at weekly intervals from October 25th, 1971 to March 29th, 1972. When a killing frost precluded further sampling, the buds were preserved by standard electron microscopic techniques. Thin sections (500 to 700 A) of the cells around the ovarious cavity were examined with a Zeiss EM-9S-2A electron microscope.

From October 25th, 1971 to January 3rd, 1972, more heterochromatin than euchromatin was in …

Meiotic And Mitotic Chromosomes Of Fritillaria Atropurpurea Nutt., John Keith Archibald

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this paper is to provide information concerning the number, structure, and behavior of chromosomes in the plant species Fritillaria atropurpurea. The material used for this investigation included both floral buds and root-tips from actively growing naturally occurring plants collected from populations in both Utah and Nevada, and root-tips from seed germinated in the greenhouse. The results obtained include a karyotype analysis of mitotic chromosomes, a discussion of meiosis, a discussion of B-chromosomes, and a series of photographs depicting the stages of mitosis and meiosis in this species.

Fritillaria atropurpurea has a diploid chromosome number of 24, …

Effects Of Land-Use Practices Upon Surface Water Quality, Carleton S. White

Biology ETDs

Surface water and precipitation samples were collected from Sept. 1972 through Aug. 1973 on the northern slopes of Mount Taylor in west-central New Mexico. The samples were analyzed for pH, conductivity, silica, calcium, magnesium, sodium, potassium, bicarbonate, alkalinity as CaCO₃, sulfate, chloride, nitrate, phosphate, and total solids. The natural baseline quality was primarily determined by the contact with the different geologic formations. The water originating on or from the basaltic terrain was of better quality and lower in dissolved solids than the water on or from the sedimentary terrain. The effects of grazing, irrigation, water impoundment, vegetation clearing, …

Perturbation Analysis Of Competition And Overlap In Habitat Utilization Between Dipodomys Ordii And Dipodomys Merriami, Gene D. Schroder

Biology ETDs

This study measures the magnitude of the competition between two nocturnal, desert rodents, Dipodomys ordii and D. merriami, on 10 unenclosed, 16 hectare plots along a narrow band of sympatry in south-central New Mexico. Population perturbations of one or the other species were periodically performed on eight of the plots, while natural population changes were monitored on two controls. The relative numerical responses of the kangaroo rats to these perturbations are thought to reflect the true magnitude of their competitive interaction.