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Agricultural Experiment Station News October 1984

Agricultural Research Division: News and Annual Reports

CONTENTS:
OUTSTANDING RESEARCH AWARDS
SUPPLEMENTAL BUDGET REQUEST
IANR FACULTY SALARIES
HOME ECONOMICS REVIEW
IANR CAPITAL CONSTRUCTION REQUEST
DISTRICT STATIONS BECOME CENTERS
RESEARCH SPONSOR NEGOTIATIONS
REPORTING RESEARCH ACCOMPLISHMENTS
FEDERAL BUDGET UPDATE
FIELD LAB SECURITY
FIELD LABORATORY NAME CHANGE?
PROGRESS ACCOMPLISHMENT REPORT
COMMODITY CHECK-OFF BOARD PROPOSALS
NEW OR REVISED PROJECTS
NEW FACULTY ON BOARD
RESEARCH GRANTS AND CONTRACTS RECEIVED SEPTEMBER 1984
USDA FY '85 COMPETITIVE RESEARCH GRANTS
NSF FELLOWSHIPS AVAILABLE
PROJECT ACCOMPLISHMENTS
CANCER AND SMOKING DISEASE RESEARCH

The Effects Of Seed Size And Maternal Origin On The Distribution Of Individual Plant Size In Ludwigia Leptocarpa (Onagraceae), Rebecca W. Dolan

Scholarship and Professional Work - LAS

Seed size is normally distributed for many annual species, while mature plant size is frequently positively skewed. A study was conducted to determine the influence of seed size and the role of genetic differences in determining relative seedling size for Ludwigia leptocarpa. Seed size had a significant effect on percentage germination and time of seed germination but no effect on dry weight or leaf area of seedlings. Seed size and spacing had a significant effect on seedling dry weight for plants grown under competition, while relative day of emergence had no effect. Familial (genetic) differences were found in average seed …

Wood Anatomy And Relationships Of Neowawraea (Euphorbiaceae), W. John Hayden, Dorthe S. Brandt

Biology Faculty Publications

Wood anatomy of three specimens of Neowawraea phyllanthoides Rock, a rare and endangered member of Euphorbiaceae endemic to the Hawaiian Islands, is described and compared with woods of other genera of subfamily Phyllanthoideae. Neowawraea has often been associated or synonymized with Drypetes Vahl. Wood of Neowawraea is diffuse porous, perforation plates are simple, imperforate tracheary elements are thin-walled septate fiber-tracheids, rays are heterocellular and crystalliferous, and axial xylem parenchyma is restricted to a few scanty paratracheal and terminal cells. In several respects these results differ from earlier published descriptions of the wood of this taxon; these earlier descriptions are shown …

Louisiana Agriculture Magazine, Fall 1984, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Expression Of Epstein-Barr Virus (Ebv) Dna And Cloned Dna Fragments In Human Lymphocytes Following Sendai Virus Envelope-Mediated Gene Transfer, David J. Volsky, Thomas Gross, Faruk Sinangil, Charles Kuszynski, Ronald Bartzatt, Timothy Dambaugh, Elliott Kieff

Chemistry Faculty Proceedings & Presentations

Purified EBV DNA and cloned DNA fragments were trapped in Sendai virus (SV) envelopes during envelope reconstitution. The DNA-loaded reconstituted envelopes (RSVE/DNA) served as gene-transfer vehicles using the capability of RSVE to fuse with normal and tumor cells. The efficiency of RSVE-mediated EBV DNA transfer into lymphoid tumor cells and fresh human lymphocytes was 5-10% of the enveloped 3H-labeled EcoRI fragment B of EBV DNA. Purified intracellular EBV (B95-8 strain) DNA induced EBV nuclear antigen (EBNA) in 0.2-1% of human lymphocytes, transiently stimulated cellular DNA synthesis, but did not fully transform cells. Cloned Sal I F1 fragment [approximately equal to …

Evaluation Of Crop Rotation For Control Of Colorado Potato Beetles (Coleoptera: Chrysomelidae) In Commercial Potato Fields On Long Island, Robert J. Wright

Department of Entomology: Faculty Publications

Leptinotarsa decemlineata (Say) densities, potato defoliation levels, and grower insecticide use were monitored in commercial potato fields with differing rotational histories on Long Island, New York. Rotation for 1 year to a nonhost grain crop (rye or wheat) significantly reduced the pest status of L. decemlineata in the following year's potato crop. Early season (late May) adult densities were reduced 95.8% in three of four comparisons in 1982 and 69.5% in two of three comparisons in 1983. In one comparison each year, early season L. decemlineata densities were low in both the rotated and nonrotated fields due to past grower …

Molecular Genetic Characterization Of The Rd-114 Gene Family Of Endogenous Feline Retroviral Sequences, Roger H. Reeves, Stephen J. O'Brien

Biology Faculty Articles

RD-114 is a replication-competent, xenotropic retrovirus which is homologous to a family of moderately repetitive DNA sequences present at ca. 20 copies in the normal cellular genome of domestic cats. To examine the extent and character of genomic divergence of the RD-114 gene family as well as to assess their positional association within the cat genome, we have prepared a series of molecular clones of endogenous RD-114 DNA segments from a genomic library of cat cellular DNA. Their restriction endonuclease maps were compared with each other as well as to that of the prototype-inducible RD-114 which was molecularly cloned from …

Body Size And Food Size In Freshwater Zooplankton, Kenneth Bogdan, John Gilbert

Dartmouth Scholarship

We used double-label liquid scintilation techniques to measure the efficiencies with which eight different sized zooplankton species ingested four celtypes relative to a standard celtype(Chlamydomonas). Efficiency ratios (ERs:clearancerateonceltypeX . clearancerateonChlam- ydomonas) on the three ultraplankton (<5 Iam in diameter) cels (a coccoid bacterium and the algae Synechococcus and Nannochloris) varied greatly among zooplankton species but were not correlated with zooplankton bodylength. Variation in ERs on a muchlarger(17x 14 um) algalcel(Cryptomonas)was only partly explained by zooplankton body length.

The Effects Of X-Irradiation On The Metamorphosis And Budding Of Aurelia Aurita, Michael John Prokopchak

OES Theses and Dissertations

With the aid of the Aurelia Metamorphosis Test System, the acute or subtle morphological and behavioral effects of irradiation on the Norfolk Aurelia aurita were described and the sensitivity to X-irradiation of those organisms receiving thyroxine and those not receiving thyroxine was compared. Levels of radiation were 0 (control), 5,000, 10,000, 15,000, 20,000 and 40,000 rads. Morphology of the ephyrae, statolith and rhopalia numbers were recorded using the light microscope, Developmental abnormalities of the scyphistomae and ephyrae were recorded with the scanning electron microscope and light microscope. Two major findings from this investigation were the absence of rhopalia and statoliths …

Correlation Studies Between Human Seminal Plasma Trace Metal Concentrations And Semen Quality, De-Shen Mei

Chemistry & Biochemistry Theses & Dissertations

Methodologies for the determination of cadmium, copper, selenium and zinc concentration in human seminal plasma have been developed using polarized Zeeman-effect atomic absorption spectroscopy. Within-run coefficients of variation (CY's) for cadmium, copper, selenium and zinc from pooled seminal plasma were found to be 8%, 5% , 4% and 0.5% at a mean (SD) concentration of 0.48 (0.04), 147 (7 .6), 53 (2. 1) and 122,900 (700) μg/ℓ, (n = 10), respectively.

Between-run CV's were determined to be 4% , 7%, 5% and 7% at a mean (SD) concentration of 2.4 (0.1), 147 (10. 1), 67.7 (3.4) and 115,800 (8300) …

Demographic Analysis And Microhabitat Relationships Of A Small Mammal Community In Clearings Of The Great Dismal Swamp, Jean Ferguson Stankavich

Biological Sciences Theses & Dissertations

Small mammals were trapped for 14 months on two grids located within a powerline right-of-way in the Great Dismal Swamp National Wildlife Refuge. During 12,354 trap nights, individuals of 10 different species were captured. They included, from highest to lowest density, Reithrodontomys humulis, Ochrotomys nuttalli, Peromyscus leucopus, Blarina brevicauda, Synaptomys cooperi, Microtus pennsylvanicus, Microtus pinetorum, Sigmodon hispidus, Mus musculus and Oryzomys palustris. The demography (densities, reproduction, home range and dispersal) of each species was analyzed when possible. Most of the species were present in relatively low densities, with a number being captured only during the latter months of the study. …

Intellectual Traditions In The Life Sciences: Ii. Stereocomplementarity, Scott F. Gilbert

Biology Faculty Works

No abstract provided.

Negative Cooperatively In The Binding Of 3-Aminopyridine Adenine Dinucleotide To L-Α-Glycero-3-Phosphate Dehydrogenase, David Wheeler

Chemistry & Biochemistry Theses & Dissertations

Glycerol-3-phosphate dehydrogenase catalyzes the reversible reaction:

NAD⁺ + G-3-P ⇌ NADH + DHAP + H⁺

The enzyme is found in organisms from E. coli to man. The primary function of the enzyme varies from organism to organism. Depending on the metabolism of the organism, the enzyme may function primarily to generate G-3-P for lipid (in organisms with a highly aerobic biosynthesis metabolism), or it may function primarily to produce NAD⁺ to support glycolysis. Complex multicellular organisms possess tissue specific isozymes of GPDH whose kinetic parameters reflect the relative importance of G-3-P and NAD⁺ production in various …

In Memoriam: Konstantin Minaevich Ryzhikov, 1912-1983, Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Obituary of Konstantin Minaevich Ryzhikov, 1912-1983.

Microbial Characteristics Of An Activated Sludge And Three Commercial Preparations After Exposure To Phenolics, Laurie Gneiding

Theses

The biological characteristics of four microbial mixtures were examined after successive exposure to phenol and 2-chlorophenol at room temperature. One of the microbial mixtures was an activated sludge from the Livingston, NJ municipal wastewater treatment plant. The other three mixtures were commercial preparations sold by Polybac Corp.(Hydrobac), Sybron Corp. (BI-CHEM DC 1006/1007), and General Environmental Sciences(LLMO).

The characteristics examined were relative counts and species of gram negative/gram positive bacteria, yeast and fungi, protozoa, and higher organisms. For the gram negative bacteria and yeasts, culturing techniques and diagnostic tubes were used to determine the predominant species. Biochemical tests were done on …

Parameters Of Growth In The Embryonic And Neonatal Chick Basilar Papilla, Douglas A. Cotanche, Kathleen K. Sulik

Scanning Electron Microscopy

The growth of the basilar papilla in the chick cochlear duct was studied utilizing light, scanning, and transmission electron microscopy. The ages of the cochleae investigated ranged from embryonic day 6 to post-hatching day 7. The changes in the length and width of the basilar papilla as well as the establishment of its spatula-like shape were correlated with the maturation of the hair cells' apical surfaces and the changes in the cellular organization of the sensory epithelium. The histological reorganization of the distal hair cell nuclei was concomitant with the broadening of the distal region of the basilar papilla and …

Catalog Of The Recent Scandentia And Primates In The Carnegie Museum Of Natural History, Suzanne B. Mclaren, Duane A. Schlitter, Hugh H. Genoways

University of Nebraska State Museum: Mammalogy Papers

The Section of Mammals, Carnegie Museum of Natural History, houses six specimens of Recent tree shrews and 611 specimens of Recent primates. The only family in the Order Scandentia is Tupaiidae. Primate families represented are Lemuridae, Lorisidae, Cebidae, Callitrichidae, Cercopithecidae, Pongidae, and Hominidae. For each specimen the following data are recorded: locality; date collected; catalog number; sex; age; nature of specimen; condition of specimen and comments. The latter category gives information on the condition of the skin and skeletal material, and gives information on the availability of the specimen.

Energy Dispersive X-Ray Analysis Of Ocean Ferromanganese Crusts Using Conventional Zaf Corrections, R. F. Commeau, J. A. Commeau, F. W. Brown, F. T. Manheim

Scanning Electron Microscopy

Ocean ferromanganese crusts are composed of interlayered phases of manganese oxide, iron oxide and oxyhydroxide, calcium carbonate/phosphate, silica, and alumino-silicates. These interlayers are so thin and fine-grained that each phase could not be isolated under the beam of a scanning electron microscope for quantitative x-ray microanalysis. A test was conducted to determine if the grain size of the phases was small enough to allow conventional ZAF techniques to be used without serious errors in the results. A "synthetic" ferromanganese crust was prepared by pelletization of a 1:1 mix of two fine-grained (<5 >micrometer) components. The mean of the energy dispersive …

An Ultrastructural Analysis Of The Physical Organization Of Collagenous (Type I) Matrices: One Determinant Of Urothelium Maintenance In Vitro, G. M. Hodges, C. Rowlatt, A. R. Howlett, L. K. Trejdosiewicz

Scanning Electron Microscopy

Collagenous matrices, used as cell culture substrata, can be prepared from different collagen types in a variety of forms using a range of polymerization procedures. Type I collagen has been most frequently used either as dried collagen films or hydrated collagen gels. Sheets of isolated bladder urothelium, when plated onto such matrices prepared from type I collagen by different polymerization methods (eg. air-drying; NaOH; NaCl; NH₃; or NH₃ followed by glutaraldehyde crosslinking) demonstrate the capability of urothelial cells to attach to a variety of differently prepared matrices irrespective of polymerization procedure. In contrast, both cell proliferation and …

The Influence Of Different Cryopreparations On The Distribution Of Ions In Bullfrog Myocard Cells, R. Meyer, M. Schmitz, K. Zierold

Scanning Electron Microscopy

Bullfrog heart muscle trabecula are shock-frozen in liquid propane cooled by liquid nitrogen and then processed for X-ray microanalysis in two different ways: 1. Freeze-drying followed by vacuum embedding. 2. Cryoultramicrotomy and freeze-drying.

Stained sections of freeze-dried, embedded tissue exhibit detailed ultrastructure, but are useless for X-ray microanalysis. Unstained, dry cut plastic-sections are suitable for X-ray microanalysis, but the ultrastructure appears faint. Higher electron optical contrast and peak-to-background ratio of X-ray spectra are generally obtained in freeze-dried cryosections. Both preparation methods show that the X-ray spectra are influenced by the quality of cryofixation. The phosphorus/potassium ratio in nuclei increases with …

Advances In Electron-Beam-Induced-Current Analysis Of Integrated Circuits, J. D. Schick

Scanning Electron Microscopy

Electron-beam-induced-current (EBIC) analysis of integrated circuits and of individual semiconductor devices has become an essential technique for semiconductor device characterization as well as for failure analysis and diagnostics. This tutorial is an update of the paper presented in SEM/1981/I on the same topic and discusses advances and new trends in the applications of EBIC in recent months. Examples of EBIC measurements on high density chips, ion-implanted junctions, and shallow diffusions in thin epitaxial layers are given. Time resolved EBIC (TREBIC) is also explained and new digital readout techniques, including computer-aided data analysis, are described as they refer to detailed analyses …

Computer-Assisted Methods For Design Optimization Of Cardiac Bioprosthetic Valves, Mohamed S. Hamid, Hani N. Sabbah, Paul D. Stein

Henry Ford Hospital Medical Journal

Excessive mechanical stresses on valvular leaflets are considered an important factor in the degeneration of cardiac porcine bioprosthetic valves. This study describes a computer-assisted numerical model of a bioprosthetic valve which estimates the magnitude and distribution of leaflet stresses. The study also describes our initial attempts to determine the influence of stent height upon leaflet stresses. Whereas lowering stent heights has been suggested to improve hemodynamics in the bioprosthetic valve region, results indicate that an increase in leaflet stresses can accompany reductions of stent height.

Automating The Expertise Of The Neuropsychologist, Kenneth M. Adams, Gregory G. Brown

Henry Ford Hospital Medical Journal

No abstract provided.

Ua66/6 Brigadoon Nature Preserve Survey Phase I, Wayne Mason, Jeff Jenkins, Kenneth Nicely, Herbert Shadowen

WKU Administration Documents

A preliminary report of an ongoing survey on the mammals, birds, vascular plants, and fungi that have been recorded at the Brigadoon Nature Preserve from November, 1983 - September, 1984.

Agricultural Experiment Station News September 1984

Agricultural Research Division: News and Annual Reports

CONTENTS:
FACULTY SALARY COMPARISON
RESEARCH EQUIPMENT ALLOCATION
FIELD LABORATORY OVERSIGHT COMMITTEE
PUBLICATION REVIEW PROCESS
ENTOMOLOGY SEARCH COMMITTEE
NEW RESEARCH FACULTY ON BOARD
NEW OR REVISED PROJECTS
NEW FACULTY APPOINTMENTS
ADDITIONAL SOYBEAN BOARD PROJECTS
AGRICULTURAL RESEARCH BROCHURE
UNL RESEARCH COUNCIL
FACULTY RETIREMENTS
FACULTY RESIGNATIONS
FORESTRY FIELD DAYS - HORNING FARM
COMPREHENSIVE PROGRAM REVIEWS
SPLINTER RECEPTION AND SEMINAR
CHANGE IN FRINGE BENEFIT RATE FOR FIGURING GRANT PROPOSALS
RESEARCH GRANTS AND CONTRACTS RECEIVED AUGUST, 1984
GILLEY'S FACULTY DEVELOPMENT LEAVE

Computer Applications In The Vascular Registry Of Henry Ford Hospital: Use Of A Simplified Access Method, Joseph P. Elliott Jr., Kunwar Kapoor, John G. Hageman, D. Emerick Szilagyi, Roger F. Smith, Michalene Mcpharlin

Henry Ford Hospital Medical Journal

The Vascular Registry of Henry Ford Hospital was initiated by one of the authors in 1957 to provide a consistent and accurate method of documenting and correlating the clinical results of the treatment of occlusive and aneurysmal arterial diseases. Data collection for each patient includes demographic information, treatment of clinical problems, and followup observations. Initially, the system consisted of keypunch cards and eventually magnetic tape processing. By 1980, it was necessary to further update the system because of the increase in patient volume, the need to minimize manual manipulations, and the demand for research data. The present system uses institutional …

Back Matter

Henry Ford Hospital Medical Journal

No abstract provided.

The Prairie Naturalist Volume 16, No. 3. September 1984

The Prairie Naturalist

Paul B. Kannowski, Editor

Nikki R. Seabloom, Assistant Editor

Douglas H. Johnson, Book Review Editor

TABLE OF CONTENTS

GRASSLAND HABITAT MANAGEMENT USING PRESCRIBED BURNING IN WIND CAVE NATIONAL PARK, SOUTH DAKOTA ▪ J. D. Forde, N. F. Sloan and D. A. Shown

BODY TEMPERATURES AND BEHAVIORAL ACTIVITIES OF HIBERNATING

PRAIRIE RATTLESNAKES, Crotalus viridis, IN ARTIFICIAL DENS ▪ K. R. Marion and O. J. Sexton

NEW VASCULAR PLANT RECORDS FOR NORTH DAKOTA ▪ D. T. Disrud, W. T. Barker and R. H. Warner

CHRISTMAS BIRD COUNTS FOR NORTH DAKOTA - 1983 ▪ R. N. Randall

COMPARISON OF PLANKTON POPULATIONS IN THREE …

Endometrium Cell Surface Abnormalities In The Syrian Hamster As A Result Of In Utero Exposure To Diethylstilbestrol, J. Gilloteaux, A. W. Steggles

Scanning Electron Microscopy

Scanning electron microscopy (SEM) was used to observe changes in the hamster endometrium cell surface following in utero pre- and/or postnatal exposure to diethylstilbestrol (DES). Some of the changes in cell surfaces are associated with alterations in cell sizes and shapes (from columnar to cuboidal and/or squamous) and in microvilli and mucous secretion. In all cases, DES treated uteri show mucosal cell surface pleomorphism, apocrine secretion and cystic accumulation of secretory material. Microvillous pleomorphism and peculiar linkages attaching one microvillus to others were investigated. Although the function and nature of such linkages is unclear, their presence seems to be more …

The Role Of The Vessels In The Growth Plate: Morphological Examination, K. Draenert, Y. Draenert

Scanning Electron Microscopy

Combined methods of light microscopy to present ossification zones by means of fluorochrome dyes make it possible to explain the contradicting presentations of the vascular system of the growth plate in the literature. The vascular system was casted with methacrylates which can be presented in the scanning electron microscope 3-dimensionally together with the trabeculae as a result of their resistance in the electron beam.

The 3-dimensional presentation in the electron microscope allows a clear distinction between the various vascular sections in the arterial flow system. In the microcorrosion casts the vessels of the epiphyseal side of the growth plate can …