Digital Commons Network^™

Cross-Reactivity Of 15 Jev Antigen-Directed Rabbit Antisera With Three Clinically Important Jev Serogroup Members: Wnv, Mvev, And Slev, Sang-Im Yun, Young-Min Lee

Animal, Dairy, and Veterinary Science Faculty Publications

Japanese encephalitis virus (JEV), the prototype member of the JEV serogroup within the genus Orthoflavivirus (family Flaviviridae), is closely related to West Nile virus (WNV), Murray Valley encephalitis virus (MVEV), and St. Louis encephalitis virus (SLEV). To characterize antigenic cross-reactivity within this group, we evaluated 15 region-specific rabbit antisera, previously generated against nearly the entire JEV protein-coding region, by immunoblotting whole-cell lysates from BHK-21 cells infected with WNV, MVEV, or SLEV, with JEV included as a reference. Six antisera (α-E^N-term, α-NS2B, α-NS3^N-term, α-NS3^C-term, α-NS5^N-term, and α-NS5^C-term) robustly recognized homologous …

Malt1 Protease Inhibition Restrains Glioblastoma Progression By Reversing Tumor-Associated Macrophage-Dependent Immunosuppression In Mice, Juliana Hofstätter Azambuja, Saigopalakrishna Yerneni, Lisa Maurer, Hannah Crentsil, Gabriela Debom, Linda Klei, Mei Smyers, Chaim Sneiderman, Kristina Schwab, Rajesh Acharya, Aivi Nguyen, Josie Emery, John Little, Jeffrey Meridew, Yijen Lin Wu, Prasanna Ekambaram, Dong Hu, Pete Gough, John Bertin, Ari Melnick, Gary Kohanbash, Riyue Bao, Peter Lucas, Linda Mcallister-Lucas

College of Life Sciences Faculty Papers

MALT1 protease is an intracellular signaling molecule that promotes tumor progression via cancer cell-intrinsic and cancer cell-extrinsic mechanisms. MALT1 has been mostly studied in lymphocytes, and little is known about its role in tumor-associated macrophages. We show that MALT1 is expressed in glioblastoma (GBM)-associated macrophages. Mechanistically, GBM tumor cells induce a MALT1-NF-κB signaling axis in macrophages, leading to enhanced macrophage migration and polarization toward an immunosuppressive ('M2-like') phenotype. Inactivation of MALT1 protease promotes transcriptional reprogramming that reduces migration and restores a macrophage anti-tumor 'M1-like' phenotype. Preclinical in vivo analysis shows that MALT1 inhibitor treatment results in immuno-reactivity of GBM-associated macrophages …

First Insular Record And Dna Barcoding Of Euscorpius Vailatii Tropea & Fet, 2015 From Trizonia Island (Fokida, Greece), Konstantinos Kalaentzis, Francesco Frigioni, Alexios Panagiotopoulos, Elias Tzoras, Styliani Minoudi, Alexandros Triantafyllidis

Euscorpius

Euscorpius vailatii Tropea & Fet, 2015 (Scorpiones: Euscorpiidae), originally described from Aitoloakarnania and Fokida (Greece), is herein reported from Trizonia Island in the Gulf of Corinth, representing the first insular record for the species. The examined specimens are consistent with the diagnostic morphology of E. vailatii. The first molecular data for the species are provided, based on both mainland and insular populations. Comparative analyses further highlight the distinctiveness of E. vailatii from geographically close congeners, while phylogenetic results indicate a sister-taxon relationship with E. erymanthius, supporting a Quaternary biogeographic history shaped by the evolution of the Gulf of …

Escherichia Coli Concentrations In Sand And Water At Tributary-Influenced Lake Michigan Beaches, Josie Elaine Kuhlman

Masters Theses

Contamination of public recreational water and sand is a human health concern, creating a management challenge for public health officials. Past research has shown that Escherichia coli can persist in sediment and can be washed into recreational water. Currently, beach monitoring practices only utilize E. coli concentrations in water samples to determine beach safety and do not consider potential exposure to sediment/sand concentrations. In this study, I investigated the relationship between E. coli concentrations in water and sand samples, which will aid public health officials in making more informed decisions about beach closures. I utilized culture-based measurements of E. coli …

Adaptive Phishing Url Detection Using Hybrid Fuzzy C-Means Clustering And Xgboost, Muntadher Mohammed Kareem, Rawaa I. Farhan

Karbala International Journal of Modern Science

Phishing attacks continue to evolve in sophistication, rendering static detection methods increasingly ineffective. Existing URL-based approaches suffer from limited adaptability to emerging phishing patterns, mislabeled training data, and insufficient validation protocols. This paper proposes a hybrid phishing URL detection system that integrates Fuzzy C-Means (FCM) clustering with XGBoost classification, enhanced by a novel Micro Adaptive Feature Extractor (MAFE). The system employs a multi-stage pipeline: feature engineering generating 36 statistical and interaction features, MAFE producing 15 adaptive features through class-aware dynamic weighting, micro-pattern detection, and entropy analysis, and FCM with K=2 clusters providing soft membership features to XGBoost. A two-pass confidence-based …

Ai-Enhanced Fluorescein Angiography Detection Of Diabetes-Induced Silent Retinal Capillary Dropout And Rna-Seq Identification Of Pre-Symptomatic Biomarkers, Yiyan Peng, Huishi Toh, Dennis Clegg, Peng Jiiang

Biological, Geological, and Environmental Faculty Publications

Objective: Retinal capillary dropout, characterized by acellular capillaries or "ghost vessels," is an early pathological sign of diabetic retinopathy (DR) that remains undetectable through standard clinical imaging techniques until visible morphological changes, such as microaneurysms or hemorrhages, occur. This study aims to develop a non-destructive artificial intelligence (AI)-based method using fluorescein angiography (FA) images to detect early-stage, silent retinal capillary dropout.

Methods: We utilized 94 FA images and corresponding destructive retinal capillary density measurements obtained through retinal trypsin digestion from 51 Nile rats. Early capillary dropout was defined as having an acellular capillary density of >= 18 counts per mm2. …

Enzymatic Synthesis Of Salicylate In Yersina Pestis Proceeds Via Intramolecular Hydroxyl Group Transfer., Clarke Miller

Georgia Journal of Science

The salicylate synthase (YbtS) of Yersinia pestis catalyzes the initial step in the biosynthesis of yersiniabactin, an iron-scavenging siderophore and critical virulence factor. While YbtS is proposed to share a  reaction mechanism with other anthranilate synthase homologs, the exact catalytic pathway has remained unresolved.  In this work, stable isotope (¹⁸O) labeling and mass spectrometry are utilized to clarify the catalytic mechanism of YbtS.  The results of the following set of experiments demonstrate that the salicylate hydroxyl group does not originate from solvent water, directly challenging previously proposed models. Instead, the lack of solvent-labeled oxygen incorporation and the absence …

Snails In The Diet Of The Cutlip Minnow, Exoglossum Maxillingua, Tess A. Taloumis, Steven L. Powers

Southeastern Fishes Council Proceedings

The Cutlip Minnow, Exoglossum maxillingua, is an important stream fish native to the eastern United States. It has an unusual lower jaw morphology. Early hypotheses suggested the jaw morphology is an adaptation for feeding on snails. Despite the publication of numerous diet studies on this species, there is no documentation of snails as prey items. During recent snorkel observations in the Roanoke River, E. maxillingua was spotted carrying snails within their mouths, prompting further investigation. An in-depth temporal examination of the diet from 145 specimens of E. maxillingua across multiple sites was conducted to further investigate the diet of …

Comparative Analysis Of Artificial Intelligence (Ai) Implications For Active Learning And Assessment At Various Curricular Levels Of Stem And Health Sciences In Developed Versus Underprivileged Countries, Vinay Munlapudi, Santanu De

FDLA Journal

Today’s all-pervasive emergence of artificial intelligence (AI) necessitates a comprehensive evaluation of its role in education, especially for occupational disciplines in health sciences and STEM. AI has reshaped student learning and instructors’ navigation of the evolving, post coronavirus infectious disease (COVID-19) global academic landscape. This study aimed at encapsulating evidence-based AI implications for student-centered, active learning and assessment at various curricular levels of science, technology, engineering, mathematics (STEM) and health sciences education in developed versus underprivileged or less developed countries. An extensive, scoping review of 65 recent, scholarly publications was conducted over six months through diverse search engines (PubMed, Google …

In Vitro Digestion Modifies Lepidium Meyenii Extract, Altering Its Major Glucosinolates, Lepidilines, Β-Carbolines, And Gut Bacterial Growth, Dominik Tarabasz, Ewa Baranowska-Wójcik, Paweł Szczeblewski, Tomasz Laskowski, Klaudia Gustaw, Marzena Furtak-Niczyporuk, Dominik Szwajgier, Rita Cristina Orihuela-Campos, Henry O. Meissner, Julia Borzyszkowska-Bukowska, Wirginia Kukula-Koch

Journal of Food and Drug Analysis

The tubers of Lepidium meyenii (commonly known as maca) have been used in the Andean cuisine for ages. The study aims to determine the behavior of the extract from the red phenotype of maca in the in vitro gastrointestinal tract model and also to define the overall impact of the extract on the microbiota. Glucosinolates, lepidilines, and a derivative of β-carboline alkaloid, i.e. 1-methyltetrahydro-β-5,6-hydride-carboline-3-carboxylic acid, as representatives of one of the main groups of metabolites were selected for the quantitative analysis after in vitro digestion at every stage of the digestion model. The results showed that lepidilines …

Using Environmental Dna (Edna) As A Tool To Detect Sharks And Rays Along The Coast Of Quintana Roo, Mexico, Sara T. Norment

All HCAS Student Capstones, Theses, and Dissertations

Elasmobranchs play a critical ecological role in marine environments, yet many populations worldwide remain poorly known and monitored. This is particularly true in Mexico despite its high biodiversity and ongoing fishing pressures. Traditional monitoring methods, such as commercial fisheries and fishery-independent surveys using longlines or nets, are often limited, leaving important knowledge gaps. Previous studies have shown that environmental DNA (eDNA), small traces of DNA left behind in the water column from feces, skin, or mucus, is a transformative, non-invasive tool for detecting elasmobranch presence and diversity across marine ecosystems.

This project used eDNA metabarcoding to detect and identify elasmobranch …

Descriptive Anatomy Of The Female Reproductive Organs In Thamin Eld’S Deer (Rucervus Eldii Thamin) And Fea’S Muntjac (Muntiacus Feae) With Utilizing Of The Glycerin Impregnation Technique For Specimen Preservation, Paisan Tienthai, Pawana Chuesiri, Itti Boonorrana, Piyaporn Kongmakee, Ampika Thongphakdee

The Thai Journal of Veterinary Medicine

The reproductive anatomy of rare cervid species remains insufficiently documented, limiting comparative studies and preservation efforts. This study describes the anatomical details in the female reproductive organs of Thamin Eld’s deer (Rucervus eldii thamin) and Fea’s muntjac (Muntiacus feae), while the application of a modified glycerin impregnation technique was achieved for specimen preservation. Dissections of female reproductive tracts revealed morphological variations that correlate with the known female reproductive characteristics of domestic and closely related species. In both species, the ovaries were characterized as small, ovoid structures, exhibiting prominent follicles or corpora lutea consistent with the stage …

Enhancing Nutrition Communication Skills Through Community-Based Learning To Create Cooperative Extension Resources, Elizabeth Lucas Combs, Anna Cason, Courtney Luecking, Heather Norman-Burgdolf

Journal of Dietetic Education

Strong communication and transferable skills are essential for future dietitians. This Best Evidence Teaching Strategy describes a community-based approach within an upper-level community nutrition course that partnered with the Cooperative Extension Service. Over six weeks, scaffolded assignments emphasized written, oral, and digital media skills, aligning with current trends in scientific communication. Students created information releases, podcasts, and short videos to translate evidence-based nutrition content into accessible formats for public audiences. Collaboration with Extension provided authentic context and motivation, as high-quality projects could be disseminated statewide.

Measuring The Empathy Of Nutrition And Dietetics Students With The Jefferson Scale Of Empathy – Health Professions Student Version: A Pilot Study, Sara Tamsukhin, Youn Seon Lim

Journal of Dietetic Education

The purpose of this study was to learn about undergraduate nutrition and dietetics students’ empathy scores. The study was a descriptive quantitative design with a web-based, cross-sectional survey. Participants were undergraduate nutrition and dietetics students from two universities who completed a survey with demographic questions, clinical experience questions, and the Jefferson Scale of Empathy – Health Professions Student version (JSE-HPS©). Seventy-one nutrition and dietetics students completed the survey. Most respondents were female, white, and non-Hispanic. The mean total empathy score was 114.69 (SD=11.09) with a range of 81 to 137. Welch’s t-test did not show significant differences by gender, ethnicity, …

Disability-Related Educational Content In Nutrition And Dietetic Programs: An Exploratory Study, Wendy Medunick, Allison Gomes, Joachim Sackey, Laura Byham-Gray, Jane Ziegler

Journal of Dietetic Education

Background: Health disparities experienced by people with disabilities (PWD) are partly attributed to inadequate knowledge, training, confidence and competence regarding disabilities among health professionals. While registered dietitian nutritionists (RDNs) are key members of the healthcare team, it is unclear how pre-professional nutrition and dietetics (ND) programs prepare future RDNs to care for PWD. This study examined the status of disability-related education within ND programs in the United States. Methods: A descriptive cross-sectional electronic survey was distributed to 570 directors of accredited ND programs. The survey, which adapted questions from a previously validated instrument and published studies, included questions assessing disability-related …

Assessing And Addressing Cultural Competence And Cultural Humility In Dietetic Education, Zoe Duran, Nicholas Slagel, Lisa Caldwell, Katie Kage

Journal of Dietetic Education

Limited theory-informed, dietetic-specific resources are available to guide the development, evaluation, and refinement of culturally competent (CC) curricula. This pilot study used a mixed-methods, theory-informed approach to evaluate CC integration within an undergraduate dietetics curriculum and examine the applicability of existing CC self-assessment tools for dietetic students. A senior-level undergraduate community nutrition curriculum was evaluated using the Tool for Assessing Cultural Competence Training (TACCT), guided by the Campinha-Bacote Model of Cultural Competence in Healthcare Delivery. James Banks’ Model of Multicultural Curriculum Reform was then applied to refine curricular content and learning activities. In addition, 74 students enrolled in a didactic …

Virtual Reality: The Future Of Didactic And Experiential Education For Nutrition And Dietetics, Michael Holik, Dexter Richardson

Journal of Dietetic Education

The integration of virtual reality (VR) into nutrition and dietetics education provides an innovative answer to challenges in accessing experiential learning. This study examined student perceptions of immersive VR experiences designed to simulate hospital foodservice operations and nutrition counseling sessions, guided by the Diffusion of Innovation Theory (DOI). A quasi-experimental design involved 55 participants who engaged with 360° VR modules featuring interactive elements, avatars representing key healthcare roles, and embedded critical-thinking questions. Results demonstrated strong support for VR’s educational value across DOI’s five key attributes: relative advantage, compatibility, complexity, trialability, and observability. Participants reported enhanced understanding of registered dietitian roles, …

Cover, Table Of Contents, About The Journal

Journal of Dietetic Education

No abstract provided.

Letter From The Professor

Scientific Kenyon: The Neuroscience Edition

No abstract provided.

Fraction-Seq: An Integrated Experimental And Computational Workflow For Determining The Localization And Abundance Of Small Non-Coding Rnas In Subcellular Compartments, Siddhartha Shah, Tess Cherlin, Yi Jing, Stepan Nersisyan, Benjamin E. Leiby, Isidore Rigoutsos

Computational Medicine Center Faculty Papers

Small non-coding RNAs (sncRNAs) have garnered considerable attention in recent years, following accumulating evidence of their critical roles in many cellular processes. Among sncRNAs, microRNAs (miRNAs) and their isoforms (isomiRs), tRNA-derived fragments (tRFs), rRNA-derived fragments (rRFs), and Y RNA-derived fragments (yRFs) account for more than 95% of all sncRNAs found in cells. Despite their critical regulatory roles, most sncRNAs remain uncharacterized because their functionalization is a lengthy and challenging undertaking. Knowing an sncRNA's abundance helps prioritize among the various choices, while knowing where it localizes in the cell greatly limits the number and identity of its potential targets and helps …

A Novel Role For The Infralimbic Cortex In Conditioned Fear Responding, Julia R. Mitchell, Samantha Tuberman, Rylin Lubash, Leticia C. N. Takasumi, Emmett Bergeron, Roberto Calitri, Mikaela A. Laine, Maryclare Pikus, Victoria Vance, Leena Ziane, Rebecca M. Shansky

Neuroscience: Faculty Publications

Freezing during Pavlovian fear conditioning is the most commonly used indicator of learned fear in rodents. Although freezing and its neural underpinnings have been widely studied, the field has largely ignored other potential indicators of learned fear. Darting, an escape-like conditioned fear response, occurs more frequently in females and is reliably predicted by a distinct behavioral phenotype: heightened shock response and decreased freezing compared to rodents that do not engage in darting. The experiments in this study sought to expand the field’s understanding of conditioned fear by investigating the neural correlates of conditioned darting, focusing on the infralimbic cortex (IL) …

Staphylococcus Aureus Urease Is Controlled By A Complex Regulatory Network To Promote Dissemination During Cauti, Jana Gomez, Jesus Duran-Ramirez, Maristhela Alvarez, Shane Cristy, Jennifer N Walker

Faculty, Staff and Student Publications

Catheter-associated urinary tract infections (CAUTIs) are one of the most common hospital-associated infections in the United States, accounting for >1 million cases annually. One CAUTI pathogen, Staphylococcus aureus, is commonly found persisting asymptomatically in the bladder of catheterized individuals, increasing these individuals' risk of developing infection. Importantly, S. aureus is not only associated with severe symptoms during CAUTI, including bacteremia and septic shock, but it also produces a common uropathogen-associated virulence factor, urease. Despite its importance, urease has only been well-studied in another uropathogen, Proteus mirabilis. While previous studies identified three S. aureus urease regulators, including CodY, …

Unphazed And Phogo: Complete Genome Sequences Of Two Ek1 Cluster Podoviridae Microbacterium Phages Containing Putative Purple Acid Phosphatase Genes, Brett M. Condon, Jacob D. Ariel, Saundra L. Godshall, Drithi Patel, Matthew T. Mcdonald, Susan M. R. Gurney

Student Papers, Posters & Projects

We report two EK1 cluster bacteriophages isolated from soil samples using the host Microbacterium foliorum. EM imaging showed that both exhibit the podovirus morphology. Genome sequencing and annotation showed similarity with many EK1 phage genomes. Our phages contain a putative PAP gene, also expressed in plants.

Contributions Of Arginine And Nitrosative Stress To Huntington’S Disease Progression, Ian D. Gaudet

Electronic Theses and Dissertations 2020 - Present

Neuroinflammation is a well-established component of Huntington's disease (HD) pathogenesis, yet the intricate molecular mechanisms that govern this process remain a topic of ongoing research and debate. This dissertation elucidates a novel pathway linking arginine metabolism and nitrosative stress to core HD pathology. We found that the arginine transporter SLC7A2 is selectively upregulated in various HD cellular models and patient samples. Our findings reveal that this upregulation drives an overactive inflammatory response, leading to abnormally high inducible nitric oxide synthase (iNOS) induction and subsequent nitric oxide (NO) production, which causes excessive protein nitrosylation and an increase in mitochondrial fragmentation.

To …

Influence Of Transboundary Wildfire Smoke On Fine Particulate Matter Concentrations At South Texas Border Schools, Sai Deepak Pinakana, Kabir Bahadur Shah, Owen Temby, Dawid K. Wladyka, Juan L. Gonzalez, Md Saydur Rahman, Katarzyna Sepielak, Amit U. Raysoni

School of Earth, Environmental, & Marine Sciences Faculty Publications

Exposure to air pollutants in indoor and outdoor school environments poses significant health risks to children due to their heightened vulnerability. Increasing wildfire frequency has intensified transboundary smoke transport, with border communities among the first to experience associated air quality impacts. While the impacts of wildfires on air quality are increasingly recognized through satellite observations and modeling, ground-based observations remain scarce in under-monitored regions. Low-cost air sensors were deployed across schools in the Roma and Rio Grande City areas of Starr County, along the U.S.–Mexico border, for 146-days from November 2023 to April 2024. The raw sensor measurements were corrected …

A Poetic Call For And A Reflection And Commentary On Clarity, Understanding, Hope, Courage, And Compassion, Carroy U. Ferguson

Psychology Faculty Publication Series

Finding a published poem I had written as a teenager, titled “The Heavens Weep.” this article uses that poem as the basis for a commentary that reflects on the state of the world today (2026),  As a commentary, the article uses the poetic and metaphoric words in the poem as a relevant "reality check" for what is still going on today.  In essence, the commentary is about a call for Clarity, Understanding, Hope, Courage, and Compassion, or what I call Archetypal Energies, Higher Vibrational Energies that operate deep within our individual and collective psyches to creatively urge us to "see" …

Formalization Of Nomenclatural Acts Proposed In Tang (2026) (Scorpiones: Buthidae, Chaerilidae, Scorpiopidae), Victoria Tang

Euscorpius

Several nomenclatural, taxonomic, and faunistic decisions put forth in Tang (2026) are formalized herein: (1) four nomina dubia, Chaerilus tessellatus Qi et al., 2005, Scorpiops langxian Qi et al., 2005, S. pococki Qi et al., 2005, and Isometrus (Reddyanus) wenjieae Huang et al., 2025; (2) two junior subjective synonyms, Scorpiops langxian = S. pococki = S. tibetanus Hirst, 1911 syn. n.; (3) one new “aspidonym”, Scorpiops obluda Tang, 2026 sp. indet. nomen nudum; and (4) removal of Scorpiops hardwickii (Gervais, 1843) and S. petersii Pocock, 1893 from the Chinese scorpiofauna. Reddyanus tibetanus (Lourenço & Zhu, 2008) is maintained …

Fisheries Research Report No. 365: Recreational Fishing For Marron In South-West Western Australia In 2026, Claire B. Smallwood, Karina L. Ryan, Eva Lai

Fisheries Research Reports

The smooth marron (Cherax cainii) (‘marron’) is endemic to freshwater dams and rivers of south-west Western Australia. Marron are managed as a single stock within the South-West Recreational Freshwater Resource and are highly vulnerable to overexploitation due to the biological characteristics of the species (e.g., low fecundity, localised populations) as well as environmental (e.g., habitat loss, reduction in rainfall) and human (e.g., fishing, land clearing) pressures on freshwater ecosystems.

Due to this vulnerability, this species has been the focus of research, monitoring and management since the 1970’s. Since 2000, annual phone-recall (2000 – 2021) and online-recall (2022 – 2026) surveys …

Evaluating The Responses Of Organic Corn Systems To Interseeded Cover Crops, Seeding Rate, And Tillage, Luvina B. Madrid

All Dissertations

Cover crop interseeding offers a potential strategy to increase plant diversity and make better use of above- and belowground resource niches within organic corn production systems. Interseeded cover crops can support agroecosystem sustainability through aboveground biomass production as well as belowground processes mediated by roots, including soil structural improvement, nutrient cycling, biological activity, and carbon storage. However, the success of interseeding depends on selecting cover crop species and management practices that provide these benefits without competing with the crop for water, nutrients, and light. Research studies were conducted to evaluate the effects of interseeded cover crop species, seeding rate, and …

Analyses Of Atheroprotective Effects From Mir-33a Deletion In Primary Vascular Smooth Muscle Cells, Achala Pokhrel

All Theses

Atherosclerosis is one of the major causes of cardiovascular disease, characterized by the accumulation of cholesterol and foam cells within the arterial walls. Foam cells were traditionally believed to originate from macrophages; however, recent studies have shown vascular smooth muscle cells are also capable of accumulating cholesterol and developing foam-cell-like characteristics. ATP-binding cassette transporter A1 (ABCA1) plays an important role in removing excess cholesterol from cells by transporting intracellular cholesterol to circulatory apolipoprotein A-I (apoAI). MicroRNA-33a (miR-33a) is known to suppress ABCA1 expression and limit cholesterol efflux. However, its role in primary aortic smooth muscle cells is unknown and not …