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Full-Text Articles in Entire DC Network
The Perceived Effect Of The Menstrual Cycle On Sleep, Mood, And Training In Ncaa Di Collegiate Athletes, Rachel Conhoff
The Perceived Effect Of The Menstrual Cycle On Sleep, Mood, And Training In Ncaa Di Collegiate Athletes, Rachel Conhoff
All-Inclusive List of Electronic Theses and Dissertations
Purpose: This study aimed to understand whether collegiate female athletes perceived that their menstrual cycle influenced sleep, mood, and training. Additionally, it looked to identify which phases of the menstrual cycle (pre-menstruation, menstruation, and post-menstruation) were associated with a positive perception of these three variables. Methods: An online questionnaire was designed and sent out to all NCAA Division I (DI) female athletes whose schools had permitted them to participate in the study. The questionnaire was a novel instrument that assessed the perception of the menstrual cycle on the three variables across the three phases. Inclusion criteria included NCAA DI female …
The Great Animal Rights Debate: From The Past Into The Present, Dayna Wiley
The Great Animal Rights Debate: From The Past Into The Present, Dayna Wiley
Celebrating Scholarship and Creativity Day (2018-)
Animal rights are not a novel concept, and everyone has their own beliefs about where animals should fit into the hierarchy of life. Not so different from today, a debate occurred in the ancient world between the Stoics, represented by Aristotle, and Plutarch, with each questioning whether animals are capable of rational thought or feelings, and therefore whether they are deserving of justice. There is much to learn from the ancient world about the modern debate on animal rights, and there is a striking comparison between this ancient debate and the modern questions of animal rights. But how similar are …
A Comprehensive Literature Review Of Barriers To Hormone Therapy Utilization: Strategies For Improving Menopause Management In Women Aged 40-60, Suhan Lee
Capstone Experience: Master of Public Health
Menopause is a natural transition marked by the cessation of ovarian activity, often accompanied by vasomotor symptoms (VMS), genitourinary syndrome of menopause (GSM), and psychological changes that significantly impact women’s quality of life. Hormone therapy (HT) has been the primary treatment for managing menopausal symptoms, particularly VMS, providing substantial relief when initiated within 10 years of menopause. Despite its effectiveness, HT usage has declined dramatically over the past two decades primarily due to barriers such as stigma, safety, misinformation, provider hesitancy, financial barriers, and cultural beliefs. This comprehensive literature review explores key factors influencing HT decision-making, including the media’s role …
Attention Deficits & The Biological Foundations Of Effective Learning Strategies, Matthew Scribner
Attention Deficits & The Biological Foundations Of Effective Learning Strategies, Matthew Scribner
Celebrating Scholarship and Creativity Day (2018-)
This capstone project will conduct a focused literature review on learning strategies for individuals with Attention Deficits Disorders. The project will explore various study strategies and modifications that have been found effective in improving learning outcomes for individuals with ADHD. Specifically, the project will delve into the neurobiological mechanisms underlying ADHD, focusing on how impairments in attention, executive function, and working memory might affect learning. It will also explore how specific modifications to study techniques can help mitigate these challenges and enhance learning outcomes. My interest in this topic stems from both my academic background as a teaching assistant in …
Mechanisms Of Behavioral Change In Hosts Of Toxoplasma Gondii: A Review, Olivia Grimsley
Mechanisms Of Behavioral Change In Hosts Of Toxoplasma Gondii: A Review, Olivia Grimsley
Senior Honors Theses
The parasite Toxoplasma gondii is a highly ubiquitous parasite that latently infects an estimated 33% percent of the global population. Toxoplasmosis is of obvious clinical significance due to the extremely high risks to the unborn who contract it congenitally. Less attention has been given to a more subtle aspect of latent infection, which is the behavioral alterations that are commonly experienced by those who contract the parasite. Infected individuals commonly display a variety of altered behaviors, such as increased aggression and risk-seeking behaviors, depression, higher tendency towards suicide and self-directed violence, a loss of fear response, and a greatly increased …
Men In Family Science Courses: Perspectives Of Family Science Faculty And Administrators, Michael Langlais, J. Mitchell Vaterlaus, Sophia Rotella
Men In Family Science Courses: Perspectives Of Family Science Faculty And Administrators, Michael Langlais, J. Mitchell Vaterlaus, Sophia Rotella
Journal of Human Sciences and Extension
The goal of this qualitative study is to describe the perspectives of higher education family science faculty and administrators regarding men’s enrollment in family science courses. University family science courses educate students on how to promote developmental potential for individuals, couples, and families. Information from these classes can assist students’ personal and professional development regardless of gender, yet there are far fewer men enrolled in these courses compared to women. Data come from 78 faculty members and 12 administrators from randomly selected universities using the National Council on Family Relations’ Degree Programs in Family Science directory. Using coding reliability thematic …
Growing Confidence: Women Forest Landowner Workshops In Georgia, Neil R. Dingley Jr., Nicholas E. Fuhrman, Kris Irwin, Elizabeth Mccarty
Growing Confidence: Women Forest Landowner Workshops In Georgia, Neil R. Dingley Jr., Nicholas E. Fuhrman, Kris Irwin, Elizabeth Mccarty
Journal of Human Sciences and Extension
The number of female forest landowners (FFLs) has been growing throughout the United States. This growth demands educational outreach for a demographic that is often behind their male counterparts in experience and education. The purpose of this exploratory study was to design and host educational workshops geared towards FFLs and to answer the following questions using quantitative and qualitative methods: (1) Are the workshops changing FFLs’ confidence in land management? (2) What are FFLs’ intentions after attending workshops? (3) Does confidence or participants’ intentions with their land vary with demographics? (4) Are workshops providing a platform to start FFL social …
Poor Sleep Quality Is Associated With Decreased Structural Coherence Of Mesolimbic Dopamine Projections In Children, William A. Walker
Poor Sleep Quality Is Associated With Decreased Structural Coherence Of Mesolimbic Dopamine Projections In Children, William A. Walker
Psychological Science Undergraduate Honors Theses
Insomnia is the second most common mental disorder that affects approximately 10% of the population. An additional one-third of the population experiences occasional symptoms of insomnia like difficulty falling asleep or staying asleep. Sleep quality is also a key indicator of overall mental and physical health; however, researchers have only begun to study the effects of the structure of the mesolimbic pathway on sleep cycle regulation and quality. Emerging research suggests that mesolimbic dopamine projections from the Ventral Tegmental Area (VTA) to the Nucleus Accumbens (NAcc) play a role in sleep-wake regulation. In this study, I analyzed data from the …
Hotter Days, Sleepless Nights: The Effects Of Climate Change On Sleep, Arianne Johnson
Hotter Days, Sleepless Nights: The Effects Of Climate Change On Sleep, Arianne Johnson
Celebrating Scholarship and Creativity Day (2018-)
This literature review explores the intersection between environmental changes and human health. It has been found that climate change through rising temperatures, extreme weather events, and poor air quality, disrupts human sleep. This lack of sleep is linked to many physical and mental health concerns in humans. By examining previous research on the topic, this review emphasizes the need to incorporate sleep health in climate adaptation and public health strategies
Predictors Of Disordered Eating Among College Students: A Multimodal Approach, Michelle Grazioli
Predictors Of Disordered Eating Among College Students: A Multimodal Approach, Michelle Grazioli
Theses, Dissertations and Culminating Projects
This study investigated potential predictors and risk factors of disordered eating behaviors and attitudes among undergraduate college students, addressing the high prevalence of disordered eating on college campuses. The sample consisted of 85 female undergraduate students who completed self-report measures of impulsivity, emotion regulation difficulties, desire for a sense of control, eating disorder behaviors and attitudes, as well as behavioral (Go/No-go task) and physiological (antisaccade task) measures of impulsivity, using food-related stimuli. Correlational, linear regression, and discriminant function analyses revealed significant associations among the self-report independent variables with eating disorder risk. Detailed findings for specific subscales of eating disorder risk, …
Involvement Of The Mpfc In Narcissism And Celebrity: A Transcranial Magnetic Stimulation Study, Sydney Ash
Involvement Of The Mpfc In Narcissism And Celebrity: A Transcranial Magnetic Stimulation Study, Sydney Ash
Theses, Dissertations and Culminating Projects
Narcissism is typically characterized by extreme self-focus, lack of empathy for others, and emotional regulation deficits. Grandiose narcissism has been linked to an increased desire for fame, while vulnerable narcissism has been linked to an increase in celebrity worship. While there is a lack of research concerning the neural correlates of desire for fame and celebrity worship, narcissism has been correlated with the medial prefrontal cortex (mPFC) in various studies. The current study aimed to identify relationships between grandiose and vulnerable narcissism, fame appeal, celebrity attitudes, need to belong, and self-esteem using six scales. We also targeted the mPFC with …
Improving Brain Health: Exercise As A Tool For Enhancing Executive Function And Reducing Stress In Older African Americans, Destiny Ellise Ramos
Improving Brain Health: Exercise As A Tool For Enhancing Executive Function And Reducing Stress In Older African Americans, Destiny Ellise Ramos
Theses, Dissertations and Culminating Projects
Older African American (AA) adults face disproportionately higher risk of stress and Alzheimer's Disease and Related Dementias (ADRD) compared to other racial and ethnic groups, yet they remain underrepresented in intervention-based research. Previous research has suggested aerobic exercise may reduce stress and benefit executive functioning, which is among the first cognitive domains to be affected in ADRD; however, no studies to date have directly tested the impact of aerobic vs. non-aerobic exercise in older AAs at high risk for developing ADRD. The current study evaluated the effects of a group-based cardio dance fitness (CDF) exercise intervention versus a strength, flexibility, …
Effects Of The Non-Stimulant Norepinephrine Reuptake Inhibitor, Atomoxetine, On Cognitive Flexibility In Healthy Adult Long-Evans Rats, Noah A. Ivak, Samantha Walkow, Christopher Knapp, Eleni Papadopoulos, Barry D Waterhouse, Rachel L Navarra
Effects Of The Non-Stimulant Norepinephrine Reuptake Inhibitor, Atomoxetine, On Cognitive Flexibility In Healthy Adult Long-Evans Rats, Noah A. Ivak, Samantha Walkow, Christopher Knapp, Eleni Papadopoulos, Barry D Waterhouse, Rachel L Navarra
Rowan-Virtua Research Day
Executive function is the control of multiple higher order cognitive processes that include attention, working memory, and cognitive flexibility. Cognitive flexibility is the ability to shift behavioral strategies
in response to changing environmental demands and task complexity. This cognitive modality is often impaired in many neuropsychiatric disorders like attention deficit hyperactivity disorder (ADHD). Deficits in cognitive flexibility are associated with dysregulation of the catecholamines, norepinephrine (NE) and dopamine (DA) within the prefrontal cortex (PFC). Atomoxetine (ATX), a non-stimulant NE reuptake inhibitor, elevates levels of NE and DA in the PFC by inhibiting the norepinephrine transporter (NET). ATX is used to …
Effects Of Social Isolation On Cecum Opioid Receptor Expression And Affective Behavior, Jordan Riser, John Tkaczynski, Nicole Shellenbarger, Maya Patel, Daniel Chandler, Daniel Manvich
Effects Of Social Isolation On Cecum Opioid Receptor Expression And Affective Behavior, Jordan Riser, John Tkaczynski, Nicole Shellenbarger, Maya Patel, Daniel Chandler, Daniel Manvich
Rowan-Virtua Research Day
BACKGROUND
Psychological stress can induce gastrointestinal symptoms and exacerbate existing gastrointestinal disorders, indicating an interaction between the central and enteric nervous systems. Central nervous system activation of opioid receptors stimulates sympathetic outflow to the enteric nervous system, delaying motility and reducing gastrointestinal secretions, producing a similar clinical presentation to IBS.
HYPOTHESIS
We hypothesize that social isolation induces anxiety states, disrupting homeostatic noradrenergic activity in the brain and gut, leading to neurophysiological changes in the cecum characterized by altered expression of opioid receptors.
METHODS
Sprague-Dawley rats were housed in either group (control) or socially isolated conditions. Affective behavior was assessed using …
Nutrition, Sleep, And Training Habits Enhance Endurance Race Performance, Anna F. Breed
Nutrition, Sleep, And Training Habits Enhance Endurance Race Performance, Anna F. Breed
Honors Theses
In recent years, marathon and ultra-marathon participation has grown substantially. Past research has shown that a range of variables influence the performance of endurance athletes. This study included participants of the Forgotten Florida 50-mile and 100-mile race and the Chattanooga Erlanger Half-Marathon and Marathon. These athletes were questioned about their sleep, nutrition, and training habits implemented for the races they completed. The results show the significance of carbohydrate consumption throughout the race for energy maintenance and shortened race times. Also, it was found that hill repeats and tempo runs brought about more successful race times for the runners who implemented …
Arthroscopy And The Future Of Surgery: Personal And Public Health Benefits, Preston T. Koski
Arthroscopy And The Future Of Surgery: Personal And Public Health Benefits, Preston T. Koski
Honors Thesis
Arthroscopy focuses on operating on the body using probes and working through keyhole incisions on the skin. This rapidly evolving field has seen many innovations in its technology, aided by advances in optical and surgical capabilities. Moreover, it presents many advantages over traditional surgery that directly impact the patient. Shorter recovery time and lessened scarring, reduced postoperative pain and opioid dependence, and less risk of infection are key benefits over open surgery. These benefits can also be applied to areas of public health. Minimizing the burden on hospital resources, reducing the environmental impact of surgery, and improving cost-effectiveness in healthcare …
The Effects Of Prenatal Cigarette Smoke Exposure On Developmental Milestones, Megan Jacobs
The Effects Of Prenatal Cigarette Smoke Exposure On Developmental Milestones, Megan Jacobs
College of Arts & Sciences Senior Theses
In Kentucky, a reported 12.7% of mothers smoke cigarettes during pregnancy. Nicotine readily crosses the placental and blood-brain barriers, with fetal blood concentrations reaching 15% higher than maternal levels. By stimulating and desensitizing nicotinic acetylcholine receptors in the autonomic nervous system, nicotine disrupts uteroplacental blood flow, potentially altering fetal growth and development.
To investigate the effects of prenatal cigarette smoke exposure (CSE) on developmental milestones, C57Bl6 mice were exposed to either cigarette smoke (CSE, n = 30) or filtered room air (Sham, n = 20) for 3 hours daily from two days before mating until the day before birth. Offspring …
Firefighters' Perceived Effectiveness Of Peer Support For Job-Related Stressors, Camryn M. Travis
Firefighters' Perceived Effectiveness Of Peer Support For Job-Related Stressors, Camryn M. Travis
Senior Honors Projects
Firefighters experience immense job-related stress. Firefighters can respond to hundreds of traumatic calls or critical incidents throughout their careers. Witnessing a critical incident can be highly stressful and mentally taxing. Therefore, some fire departments offer mental health resources such as Critical Incident Stress Management/Debriefing (CISM/D) and Peer-group support to help firefighters cope with stressful incidents. Additionally, firefighters may seek professional mental health treatment. To investigate firefighters’ perceptions regarding the effectiveness of these resources, an anonymous survey of Rhode Island firefighters was conducted. The sample included 51 firefighters (n = 51), was mostly male (90.2%), and self-identified as predominantly white (94.1%) …
Simulating Chill: Exploring The Cognitive And Therapeutic Potential Of Cold Vr Environments, Jessica Turner, Piper Hutson, James Hutson
Simulating Chill: Exploring The Cognitive And Therapeutic Potential Of Cold Vr Environments, Jessica Turner, Piper Hutson, James Hutson
Faculty Scholarship
This study investigates the cognitive and therapeutic potential of immersive virtual reality (VR) environments designed to simulate cold conditions. Through the engagement of participants through multisensory stimuli—including vivid visual representations of the Athabasca Glacier, auditory effects of icy winds, and corresponding haptic feedback—the research evaluates neurological and physiological responses associated with attention, emotional regulation, and stress modulation. Participants experienced virtual scenarios featuring icy winds and snow, activating specific neurological pathways involving the occipital lobe, primary visual cortex, superior colliculus, and insula, thus reinforcing sensory integration. Through predictive coding, the anterior insula and hypothalamus were engaged, prompting thermoregulatory simulations and subconscious …
Identifying Cognitive, Affective, And Developmental Mechanisms Linking Threat And Deprivation With Adolescent Psychopathology, Ekaterina Sadikova, David G. Weissman, Maya L. Rosen, Elise Robinson, Liliana J. Lengua, Margaret A. Sheridan, Henning Tiemeier, Katie A. Mclaughlin
Identifying Cognitive, Affective, And Developmental Mechanisms Linking Threat And Deprivation With Adolescent Psychopathology, Ekaterina Sadikova, David G. Weissman, Maya L. Rosen, Elise Robinson, Liliana J. Lengua, Margaret A. Sheridan, Henning Tiemeier, Katie A. Mclaughlin
Neuroscience: Faculty Publications
Background: The mechanisms linking early-life adversity with psychopathology over the life-course are complex. In this prospective study, we collectively examined cognitive, affective, and developmental mediators previously found to individually link childhood threat and deprivation experiences to adolescent psychopathology to identify the most potent mechanisms. Methods: Data came from a community sample of 227 children (mean child age 11.5 ± 0.5 years, 48.5% female) from the Seattle metro area with recruitment designed to reflect diversity in family income. Candidate mechanisms included self-rated pubertal development and task-measured attention bias to threat, emotion regulation, theory of mind, fear learning, inhibitory control, language ability, …
Supplementation Of Magnesium Glycinate: Testing The Acute Effects Of Magnesium Glycinate On The Stress Levels Of College Students, Meagan Bubeck
Supplementation Of Magnesium Glycinate: Testing The Acute Effects Of Magnesium Glycinate On The Stress Levels Of College Students, Meagan Bubeck
Honors Theses
he usage of supplements and vitamins to increase health has become a common practice in the United States. However, the true effects are not always well-known and can differ across brands, population, and other factors. In college, many students are functioning in high stress conditions, and some may result to going on anti-anxiety or depression medications. To see if Magnesium supplementation could be a potential first step in improving the stress levels present in the student population, the effects of Mg were studied in a population of 10 participants. Participant data was studied in both an Mg and placebo condition …
Movement Ecology Of Sandhills Chub, A Carolina Endemic, Zachary Adam Ramsey
Movement Ecology Of Sandhills Chub, A Carolina Endemic, Zachary Adam Ramsey
Electronic Theses and Dissertations
Fish movement studies have traditionally focused on species that support commercial and recreational fisheries, but recently more focus has been given to non-game, stream fishes because of their recognized importance in maintaining biodiversity within these ecosystems. Stream fish movement is described as being “restricted” and following a leptokurtic distribution via diffusive spread related to environmental and biological factors. Many studies of stream fish movement ecology in North America have occurred in high-gradient streams with coarse substrate in mountains or in streams of the interior plains. Sandhills streams of the southeastern USA are characterized as warm-water, low gradient, and dominated by …
Heterotopias Of Confinement: Zoo Architecture And The Loss Of Elephant Agency, Clayton Gill Beasley
Heterotopias Of Confinement: Zoo Architecture And The Loss Of Elephant Agency, Clayton Gill Beasley
Electronic Theses and Dissertations
Zoos functions as a site where human-animal relationships are structured through built environments that mediate control and representation. This study examines zoos as heterotopias—spaces where power and surveillance are negotiated between human observers and captive nonhuman animals. While zoos are often framed as spaces of conservation and education, their architectural design reinforces hierarchies of observation and confinement, shaping both human perceptions of nature and the lived experiences of nonhuman animals. This research focuses on Asian elephants as a critical species within this framework, exploring how their captivity illuminates tensions between human-imposed structures and nonhuman autonomy. Drawing from theories of heterotopia, …
Assessing Sleep Quality In A Preclinical Model Of Low Back Pain Through Novel Noninvasive Methods, Lydia C. Saltz
Assessing Sleep Quality In A Preclinical Model Of Low Back Pain Through Novel Noninvasive Methods, Lydia C. Saltz
Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research
Chronic low back pain (LBP) has widespread negative impacts on quality of life. Current treatments for chronic LBP fail to provide long-term efficacy and motivate the need to understand key drivers of poor patient outcomes. Mounting evidence indicates that sleep disturbances and chronic LBP have a strong reciprocal relationship. Women may be particularly vulnerable to the negative impacts of the sleep-pain axis, exhibiting greater prevalence in both conditions. However, the relationship between sleep, pain, and sex remains understudied. Preclinical models can be used to parse out characteristic sleep features that degrade with pain, which can be further broken out by …
Acetylcholine Release In The Dorsal Lateral Straitum During Social Approach Task, Taylor Good, Oyku Dinckol, Noah Wenger, Jake Caselli, Munir Gunes Kutlu
Acetylcholine Release In The Dorsal Lateral Straitum During Social Approach Task, Taylor Good, Oyku Dinckol, Noah Wenger, Jake Caselli, Munir Gunes Kutlu
Rowan-Virtua Research Day
Nicotine Decreases ACh Release in the DS leading to a Serenic Effect during Social Approach Task
Acetylcholine (ACh) regulates attention, learning, and stress responses (Benarroch E. E., 2012). The dorsal striatum (DS), essential for stimulus-response learning, is also linked to anxiety and compulsivity (Casado-Sainz et al., 2021). We used behavioral pharmacology and in vivo fiber photometry to study ACh responses during social approach tasks in the dorsal striatum. Male and female C57BL/6J mice underwent stereotaxic surgeries to inject a fluorescent ACh sensor (ACh.Sn.Fr) and implant fiber optic cannulas in the DS. Using in vivo fiber photometry, we tracked ACh release …
Modulation Of Opioid Receptor Expression In The Ventral Tegmental Area Following Stress Exposure In Rats, Maya Patel, John Tkaczynski, Jordan Riser, Nicole Shellenbarger, Jin Park, Daniel Manvich, Daniel J. Chandler
Modulation Of Opioid Receptor Expression In The Ventral Tegmental Area Following Stress Exposure In Rats, Maya Patel, John Tkaczynski, Jordan Riser, Nicole Shellenbarger, Jin Park, Daniel Manvich, Daniel J. Chandler
Rowan-Virtua Research Day
Background: The ventral tegmental area (VTA) contains dopamine-producing neurons projecting to various brain regions. VTA dysfunction is linked to addiction and depression. Delta opioid receptor upregulation relates to chronic pain, inflammation, cancer, and early adversity. Kappa receptors in the VTA influence stress, drug-seeking, mood, and reward. Notably, chronic substance abuse upregulates the κ-receptor/dynorphin system, and the opioid system regulates social interaction needs.
Hypothesis: Stress will upregulate opioid receptors.
Methods: We compared group-housed and individually-housed rats using stress tests, analyzing behavior and quantifying VTA opioid receptor expression.
Results: Social isolation upregulated delta and kappa opioid receptors in …
Protein Intake And The Wellbeing Of Postmenopausal Women: Investigating Sleep, Mood, And Quality Of Life, Aleesha Imran
Protein Intake And The Wellbeing Of Postmenopausal Women: Investigating Sleep, Mood, And Quality Of Life, Aleesha Imran
Biological Sciences Undergraduate Honors Theses
Postmenopausal women commonly experience declines in sleep quality, mood, and overall wellbeing, with limited non-pharmacological options available to address these concerns. This randomized controlled trial investigated whether daily beef consumption as part of a higher-protein diet could improve sleep, mood, and quality of life over four weeks. Participants were randomized to either a standard protein intake control group or a treatment group consuming a high-protein diet with daily lean beef.
The treatment group showed modest improvements in subjective sleep quality and perceived health compared to controls, although these changes were not statistically significant. Fatigue, however, significantly decreased in the treatment …
The Relationship Between Oxidative Stress And Partner Opposition In Conflict Resolution, Jenna Thomas
The Relationship Between Oxidative Stress And Partner Opposition In Conflict Resolution, Jenna Thomas
Biological Sciences Undergraduate Honors Theses
Given the inevitability of conflict in romantic relationships, understanding its biological impact is crucial for developing conflict resolution strategies that promote physical health. While many studies highlight relationship conflict’s negative effects on bodily systems, few examine how specific facets of conflict, such as opposition, impact the body. Partner opposition may contribute to oxidative stress, a biological marker associated with poor physical health, by activating the amygdala. This study investigated the association between partner opposition and oxidative stress in 126 couples expecting their first child. Participants attended a lab session where problem-solving discussions were recorded to assess oppositional behavior and urine …
A Nano-Scale Approach To Controlled Apoptosis In Brain Cancer Through Magneto-Mechanical Actuation, Sakura Rieck
A Nano-Scale Approach To Controlled Apoptosis In Brain Cancer Through Magneto-Mechanical Actuation, Sakura Rieck
Biological Sciences Undergraduate Honors Theses
In this study, we investigate the effects of cell therapy and magneto-mechanical actuation (MMA) to treat glioblastoma (U87). MMA approach employs super low-frequency alternating magnetic fields (AMF) to actuate intracellular PEGylated superparamagnetic iron oxide nanoparticles (PEG-SPIONs). Magnetic forces are translated into mechanical agitation on PEG-SPIONs, which can disrupt key cellular components. We explored two ways in which the MMA approach can be used to combat cancer growth. First, we investigated MMA directly in U87 cells. Preliminary observations indicate that PEG-SPIONs are intracellularly taken up by the U87 cells via micropinocytosis. Cell-counting kit-8 toxicity assays revealed a cytotoxic effect of MMA …
Investigating The Effects Of Sod1 Knockout On Oxidative Stress And Stress Coping Styles In Zebrafish Using Crispr Technology, Tai Prauner, Ryan Wong
Investigating The Effects Of Sod1 Knockout On Oxidative Stress And Stress Coping Styles In Zebrafish Using Crispr Technology, Tai Prauner, Ryan Wong
Theses/Capstones/Creative Projects
Reactive oxygen species are byproducts of cellular metabolism that, when not efficiently neutralized, can damage DNA, proteins, and lipids. Superoxide dismutase 1 (SOD1) is an antioxidant enzyme that converts superoxide radicals into less harmful molecules. While its molecular function is well documented, the role of SOD1 in shaping stress-related behavior remains underexplored. This project aimed to establish foundational tools for studying the behavioral impact of SOD1 knockout across proactive and reactive stress-coping styles in zebrafish.
To support future gene-editing experiments, microinjection and breeding protocols were refined across wildtype AB, proactive, and reactive zebrafish lines. No embryos were injected with CRISPR-Cas9 …