Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

Life Sciences

Institution
Keyword
Publication Year
Publication
Publication Type
File Type

Articles 5041 - 5070 of 44747

Full-Text Articles in Entire DC Network

Water Quality For Livestock, Department Of Primary Industries And Regional Development, Western Australia May 2024

Water Quality For Livestock, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Measure water quality and quantity to effectively plan and monitor water supplies for livestock. If water quality is poor, livestock may drink less than they need, or rarely, may stop drinking altogether. When animals drink less, they will eat less and lose condition, and if they are lactating, their milk production will reduce or cease.

Water quality for livestock in Western Australia is most affected by water salinity, and the presence of water contaminants such as blue-green algae, organic material, heavy metals and chemicals.


One Year Follow Up Of A Cross-Sectional Study Of Hookworm Infection And Response To Deworming In Beposo, Ghana, Taylor Clarkson May 2024

One Year Follow Up Of A Cross-Sectional Study Of Hookworm Infection And Response To Deworming In Beposo, Ghana, Taylor Clarkson

Capstone Experience: Master of Public Health

Hookworms are soil-transmitted helminths that infect over 500 million people worldwide, primarily those in low-income countries. Infection with hookworm can cause anemia, malnutrition, and stunted physical and cognitive development. Deworming campaigns have attempted to combat hookworm infections in endemic areas for decades. However, there is growing variability in the effectiveness of deworming drugs, and post-treatment reinfections are abundant. There are significant gaps in our understanding of critical host-parasite factors that mediate hookworm infection, reinfection, and response to deworming treatment in endemic communities. A five-year prospective study was undertaken to better understand drivers of hookworm infection in rural Ghana and thus …


Magnetic Three-Dimensional Graphene: A Superior Absorbent For Selective And Sensitive Determination Of Nitrite In Water Samples By Ion-Pair Based-Surfactant-Assisted Solid-Phase Extraction Combined With Spectrophotometry, Mina Vasheghani Farahani, Sajad Karami, Hassan Sereshti, Shokouh Mahpishanian, Somayeh Koupaei Malek, Shahabaldin Rezania May 2024

Magnetic Three-Dimensional Graphene: A Superior Absorbent For Selective And Sensitive Determination Of Nitrite In Water Samples By Ion-Pair Based-Surfactant-Assisted Solid-Phase Extraction Combined With Spectrophotometry, Mina Vasheghani Farahani, Sajad Karami, Hassan Sereshti, Shokouh Mahpishanian, Somayeh Koupaei Malek, Shahabaldin Rezania

Nutrition, Dietetics, and Food Science Student Research

A straightforward, fast and efficient analytical method was developed which utilizes a magnetic composite called three-dimensional graphene (3D-G@Fe3O4) as an adsorbent to recover nitrite ions (NO2–) from environmental water samples. The investigation into the synthesized adsorbent contained an examination of its morphology, chemical composition, structural attributes, and magnetic properties. This comprehensive analysis was conducted using various instrumental techniques, including Fourier transform-infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), Raman spectroscopy, X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Barrett-Joyner-Halenda (BJH), and vibrating sample magnetometry (VSM). The adsorbent surface was activated by adding cetyltrimethylammonium bromide (CTAB) to the …


Effect Of Short-Term Exposure To Pesticide Mixture On Tissue Architecture, Body Fluid Conditions, Nitrative Stress And Osmoregulation Of American Oyster, Crassostrea Virginica, Asif Ahmed May 2024

Effect Of Short-Term Exposure To Pesticide Mixture On Tissue Architecture, Body Fluid Conditions, Nitrative Stress And Osmoregulation Of American Oyster, Crassostrea Virginica, Asif Ahmed

Theses and Dissertations

Pesticides are used widely to control weeds and pests in agricultural settings but harm non-target aquatic organisms. We examined the effect of short-term exposure (one week) to pesticide mixtures (low dose: 0.5 μg/L Roundup®, 0.5 μg/L 2,4-D, and 0.4 μg/L atrazine; high dose: 1 μg/L Roundup®, 1 μg/L 2,4-D, and 0.8 μg/L atrazine) on American oyster (Crassostrea virginica, an important shellfish and seafood species) under controlled laboratory conditions. Histological analyses demonstrated the atrophy in oysters' gills, digestive glands, and connective tissue. The protein concentrations and glucose levels dropped significantly in the high-dose treatment. The number of hemocytes in connective tissue …


Effects Of Maternal Infant-Directed Sensory Cues On Early Weaned Piglet Behavior, Mattie Still May 2024

Effects Of Maternal Infant-Directed Sensory Cues On Early Weaned Piglet Behavior, Mattie Still

Animal Science Undergraduate Honors Theses

In the commercial pig production industry, weaning is a stressful time. Stress is related to loss in production due to increased incidence of illness, increased aggression, and reduced rate of gain, which results in economic losses for the producer. The purpose of this continuous behavior study was to explore the effects of provisional own-mother olfactory and auditory stimuli on early weaned piglet behavior and growth. During days 7-14 postpartum, the grunting of 12 individual sows was recorded while their piglets nursed. The audio files were put into a single loop with grunting occurring for 20 minutes followed by 60 minutes …


Forage News [2024-05], University Of Kentucky May 2024

Forage News [2024-05], University Of Kentucky

Forage News

No abstract provided.


Searching For Regulators Of Catalase Activity Using Enzymatic Screens, Aram Joseph Alaxanian May 2024

Searching For Regulators Of Catalase Activity Using Enzymatic Screens, Aram Joseph Alaxanian

Legacy Theses & Dissertations (2009 - 2024)

Catalase is found in a plethora of organisms and plays a crucial role in safeguarding cells against oxidative stress-induced damage to proteins, RNA, and DNA. Catalase catalyzed the decomposition of hydrogen peroxide (H2O2) into oxygen and water. Deficiencies in catalase have been linked to pathological conditions such as Diabetes, Alzheimer's, and Cancer, underscoring its significance in cellular health. In bacteria like Escherichia coli, catalase is encoded by two genes, KatG and KatE, both subject to regulation through transcription, translation, and post-translational modification. Our study focused on assessing the catalase activity of mutants lacking stress response genes (e.g., lexA, soxR, soxS, …


Interplay Between Cellular Stress Mechanisms And Immune Responses In Restricting Zika Virus Infection, Pheonah Badu May 2024

Interplay Between Cellular Stress Mechanisms And Immune Responses In Restricting Zika Virus Infection, Pheonah Badu

Legacy Theses & Dissertations (2009 - 2024)

Flavivirus infections pose significant global health risks, often leading to serious neurological and congenital complications such as microcephaly. These viruses induce complex cellular stress responses pathways encompassing the Unfolded Protein Response (UPR), Integrated Stress Response (ISR), autophagy, apoptosis, and the antiviral immune response, which dictate cell fate by either promoting survival to combat virus-induced damage or initiating cell death under prolonged stress. Despite the antagonizing functions, flaviviruses employ various subversion strategies, facilitated by viral proteins, to ensure successful infections even in the presence of activated cellular responses. Recent studies highlight dynamic interactions among stress response pathways during infection, emphasizing the …


The Associations Between Maternal Perinatal Depression And Pre-Pregnancy Obesity With Child Neurodevelopment: A Systematic Review, Brooke Connolly May 2024

The Associations Between Maternal Perinatal Depression And Pre-Pregnancy Obesity With Child Neurodevelopment: A Systematic Review, Brooke Connolly

ALL - Honors Theses

This systematic review examined the association between maternal pre-pregnancy obesity and perinatal depression, and the joint effect of these conditions with child neurodevelopment. PubMed was systematically searched for studies published between January 2018-December 2023. Studies were screened and included if they were cohort studies and human studies in English. Of the 41 studies identified examining maternal perinatal depression and 45 studies identified examining maternal pre-pregnancy obesity, 12 and 9 studies were included in this systematic review, respectively. No studies were identified examining the joint association of these factors with child neurodevelopment. Overall, the studies showed an inverse association between maternal …


Effects Of Cogongrass Invasion On Soil Biota And Nutrient Loads In A Longleaf Pine Savanna, Jordan Werneth May 2024

Effects Of Cogongrass Invasion On Soil Biota And Nutrient Loads In A Longleaf Pine Savanna, Jordan Werneth

Honors Theses

The longleaf pine (Pinus palustris) ecosystem, characterized by its namesake pine species and diverse community of understory vegetation, is a hotspot of biodiversity in the southeastern United States. The longleaf pine savanna is differentiated from other longleaf systems through the combination its unique assemblage of herbaceous understory vegetation, wide-open canopy, and distinct hydrogeological composition. Longleaf pine ecosystems, including savannas, are facing significant decline from their historic range due to anthropogenic interference including fire suppression, habitat fragmentation, deforestation, and the introduction of invasive species such as cogongrass (Imperita cylindrica).

Cogongrass, a nonnative grass species introduced to the …


Bilateral Asymmetry In The First Metacarpal: The Influence Of Handedness In Humans And Non-Human Primates, Lucyna A. Bowland May 2024

Bilateral Asymmetry In The First Metacarpal: The Influence Of Handedness In Humans And Non-Human Primates, Lucyna A. Bowland

Graduate Theses and Dissertations

Muscle attachment sites (entheses) are often used to infer soft tissue anatomy and reconstruct behaviors within skeletal populations, but there remains a significant debate about whether and to what degree muscle use is accurately reflected in bony morphology. One of the most difficult problems in establishing the relationship between variation in muscle use and entheseal development is the inability to exclude a variety of external factors thought to influence enthesis development, including age, sex, body mass, and activity patterns. One way around this is to take advantage of the fact that humans are handed (preferentially using one hand over another, …


Nebline, May 2024 May 2024

Nebline, May 2024

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Weed Control in Pastures and Hayfields by John Nelson

Navigating Nutrition Information: Separating Fact from Fiction by Alyssa Havlovic

Recipe of the Month: Pepper Steak Sandwich by Alyssa Havlovic

Care for New Bedding Plants by Sarah Browning

Garden Guide: Things to Do This Month

Cash Rent Survey

No Matter Where You Live, Submit a Request Before You Dig! by Becky Schuerman

4-H Achievement Celebration

8 Lancaster County 4-H’ers Modeled Their Sewn Garments at Omaha Fashion Week

4-H Events Open to All Youth

Support 4-H by Eating Out, May 1 & 2

Support 4-H During “Give to Lincoln Day,” May 30 …


Determining The Sap Yield Of Three Maple Species And The Viability Of A Maple Syrup Industry In Northern Utah, Jesse Mathews May 2024

Determining The Sap Yield Of Three Maple Species And The Viability Of A Maple Syrup Industry In Northern Utah, Jesse Mathews

All Graduate Theses and Dissertations, Fall 2023 to Present

Maple syrup is an agricultural product produced from the sap exuded by maple trees. All maples can produce sap, and in Utah, the maples include the two native species bigtooth maple (Acer grandidentatum) and boxelder (Acer negundo), and the introduced species Norway maple (Acer platanoides). Little is known about the sap production of these species, and this research aims to examine the volume of sap yield and sugar content of these maples, evaluate the performance of bucket and tubing collection systems, and explore the potential economics of a maple industry in the state. Research …


Design Of Small-Scale Milk Processing Facility, Luke Bond, Robert Stenzel, Ry Steffen, John Van Nieuwenhuyse, Rossana Villa-Rojas, Terry Howell Jr., Tami M. Brown-Brandl, Forrest Kievit May 2024

Design Of Small-Scale Milk Processing Facility, Luke Bond, Robert Stenzel, Ry Steffen, John Van Nieuwenhuyse, Rossana Villa-Rojas, Terry Howell Jr., Tami M. Brown-Brandl, Forrest Kievit

Department of Agricultural and Biological Systems Engineering: Masters Project Reports

This project was intended to investigate the feasibility of a proposed modular dairy processing facility collocated with a small dairy on the University of Nebraska–Lincoln’s Innovation Campus. Priority was placed upon cost savings for equipment to be used in processing. Process flow diagrams, floor plans, production schedules, risk assessments, and plans for wastewater treatment were all developed to address the needs of the plant should it be built. A Monte Carlo simulation was conducted to evaluate the timeline for breaking even financially, resulting in the potential for a payback period of 4.5 years. The 4.5-year payback period was based on …


Moisture Sorption And Quality Characteristics Of Instant Rice, Anna Mays, Griffiths Atungulu May 2024

Moisture Sorption And Quality Characteristics Of Instant Rice, Anna Mays, Griffiths Atungulu

Food Science Undergraduate Honors Theses

Market demand for instant rice--a processed form of rice that is cooked and dehydrated before it is sold--has risen tremendously and become a significant component of the rice industry. Compared to freshly cooked rice, the quality of instant rice (in terms of texture, color, aroma, etc.) is much lower. There exists little information regarding how instant rice’s storage conditions affect its quality. Such information may be elucidated by studying sorption isotherms which describe the storage temperature, relative humidity, and instant rice moisture relationships.

The purpose of this study was to generate moisture sorption isotherms of instant rice for temperatures and …


On-Farm Nitrogen And Irrigation Management Strategies To Protect Groundwater Quality In The Bazile Groundwater Management Area, Arshdeep Singh May 2024

On-Farm Nitrogen And Irrigation Management Strategies To Protect Groundwater Quality In The Bazile Groundwater Management Area, Arshdeep Singh

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Increasing groundwater nitrate (NO3-N) contamination has raised significant environmental and health concerns in the irrigated sandy soils of Nebraska. Four studies were conducted to evaluate the impact the impact of 1) static (NE Yield Goal) vs. dynamic nitrogen (N) recommendations tools (Maize-N, Canopy Reflectance Sensing, Granular, and Adapt-N), 2) three N (optimum, suboptimum, and low) and irrigation rates (farmer’s full irrigation (FIT), 80% FIT, and 60% FIT), 3) conventional N sources vs. enhanced efficiency fertilizers (EEFs), and 4) multiple N splits (2, 3, 4, and 5-N splits) on agronomic (maize yield), environmental (NO3-N leaching), and economic returns (return …


The Impact Of One Night Of Sleep Restriction On Perception Of Subsequent Aerobic Exercise Performance, Erin G. Meier May 2024

The Impact Of One Night Of Sleep Restriction On Perception Of Subsequent Aerobic Exercise Performance, Erin G. Meier

Senior Honors Projects, 2020-current

Introduction: Previous research has established that poor sleep quality negatively impacts aerobic exercise performance. A majority of that research has examined extreme sleep disruption like complete sleep deprivation or multiple nights of reduced sleep. Little research exists examining the effect of a singular night of sleep restriction (SR) on performance, and there is even less research on the associated perceptual feelings and outcomes from SR that may influence performance. Previous research from our laboratory has suggested that a single night of SR may impair performance, but did not account for sleep inertia or examine any perceptual factors. Therefore, the …


Yield And Nutritive Quality Of Fresh And Ensiled Reduced Lignin Alfalfa In Monoculture And Diculture With Novel Tall Fescue, Morgan Boss May 2024

Yield And Nutritive Quality Of Fresh And Ensiled Reduced Lignin Alfalfa In Monoculture And Diculture With Novel Tall Fescue, Morgan Boss

All Theses

The objectives of this research were to: (1) evaluate the yield, nutritional value, and digestibility of fresh and ensiled reduced lignin alfalfa (RLA; Medicago sativa L.) in monoculture and when mixed with novel endophyte-infected tall fescue (F; Festuca arundinacea) in comparison to a conventional alfalfa (CA) cultivar; and (2) evaluate ensiled samples under varying DM concentrations; with or without inoculant. A trial was prepared as a randomized complete block design with 4 replicates per treatment. Treatments included reduced lignin alfalfa (RLA), conventional alfalfa (CA) and tall fescue (F) plots as well as RLA/F and CA/F plots. Plots were harvested on …


Effect Of Source Of Trace Minerals On Nutrient Digestibility And Rumen Fermentation Of Dairy Cows, Cesar Velasquez Rios May 2024

Effect Of Source Of Trace Minerals On Nutrient Digestibility And Rumen Fermentation Of Dairy Cows, Cesar Velasquez Rios

All Theses

none


Transcriptomic Profile Of Armillaria Root Rot Fungi Affecting The U.S. Stone Fruit Industry, Jared Weaver May 2024

Transcriptomic Profile Of Armillaria Root Rot Fungi Affecting The U.S. Stone Fruit Industry, Jared Weaver

All Theses

Armillaria Root Rot (ARR) is a significant replant disease threatening the future of the United States stone fruit industry. Economic loss in South Carolina peach orchards is estimated to be $8M annually due to approximately 3-4% of annual tree deaths caused by ARR on replant sites. Management solutions are insufficient and can at best extend the life of the orchards on replant sites. The only solution to solving the ARR problem, apart from abandoning the replant site for peach production, is planting orchards on ARR-resistant rootstock. Significant progress has been made towards providing growers with genetically resistant rootstock, however, for …


The Effects Of Fermented Vegetables On The Gut Microbiota For Prevention Of Cardiovascular Disease, Melissa Baron, Bin Zuo, Jianmin Chai, Jiangchao Zhao, Alireza Jahan-Mihan, Judy Ochrietor, Andrea Y. Arikawa May 2024

The Effects Of Fermented Vegetables On The Gut Microbiota For Prevention Of Cardiovascular Disease, Melissa Baron, Bin Zuo, Jianmin Chai, Jiangchao Zhao, Alireza Jahan-Mihan, Judy Ochrietor, Andrea Y. Arikawa

Animal Science Faculty Publications and Presentations

This study investigated the impact of regular consumption of fermented vegetables (FVs) on inflammation and the composition of the gut microbiota in adults at increased risk for cardiovascular disease. Eighty-seven adults ages 35–64 were randomized into an FV group, who consumed 100 g FVs daily at least five times per week for eight weeks, or a usual diet (UD) group. Blood and stool samples were obtained before and after the intervention. Dependent samples t tests and adjusted linear models were used for within- and between-group comparisons. The mean age and body mass index of participants were 45 years and 30 …


Influence Of Finishing Systems On Carcass Characteristics, Composition, And Fatty Acid Profile Of Bison Bulls, Clay J. Newton, Lydia M. O'Sullivan, Keith R. Underwood, Judson K. Grubbs, Christina E. Bakker, Kristi M. Cammack, Thu Dinh, Carter Kruse, Amanda D. Blair May 2024

Influence Of Finishing Systems On Carcass Characteristics, Composition, And Fatty Acid Profile Of Bison Bulls, Clay J. Newton, Lydia M. O'Sullivan, Keith R. Underwood, Judson K. Grubbs, Christina E. Bakker, Kristi M. Cammack, Thu Dinh, Carter Kruse, Amanda D. Blair

Animal Science Research Report 2024

No abstract provided.


Exploring The Effects Of N,N-Dimethylbiguanide And Ω-3 Pufas On Muscle Stem Cell Differentiation, Enayah Alhshim May 2024

Exploring The Effects Of N,N-Dimethylbiguanide And Ω-3 Pufas On Muscle Stem Cell Differentiation, Enayah Alhshim

Graduate Theses and Dissertations

The impacts of omega-3 (ω-3) fatty acid and metformin hydrochloride (Met-HCl) on muscle stem cells differentiation, mimicking the fetal muscle development under gestational diabetes mellitus (GDM), were investigated. ω-3 fatty acids have been known for their ability to regulate adipogenic transcription factors such as Sterol Regulatory Element-Binding Protien (SREBPs) and Peroxisome Proliferator-Activated Receptors (PPARs). This regulation resulted in gene expression modification for lipid metabolism and adipocyte differentiation, thus preventing excessive fat accumulation. Additionally, emerging evidence suggested that ω-3 fatty acids may also exert direct effects on myogenic processes that influence muscle stem cells proliferation, differentiation, and maturation. Meanwhile, Met-HCl, an …


5-Year Strategic Direction, 2024–2028: Institute Of Agriculture And Natural Resources, University Of Nebraska–Lincoln, Institute Of Agriculture And Natural Resources University Of Nebraska-Lincoln May 2024

5-Year Strategic Direction, 2024–2028: Institute Of Agriculture And Natural Resources, University Of Nebraska–Lincoln, Institute Of Agriculture And Natural Resources University Of Nebraska-Lincoln

Institute of Agriculture and Natural Resources: Institute Publications

IANR: Integrating Learning, Discovery, and Engagement for an Even Better Nebraska

Includes an introduction, description of the components of the Institute of Agriculture and Natural Resources at the University of Nebraska–Lincoln, the IANR pillars, stratgeic aims for 2024–2028, and a section on implementation.


Dispersal Movement And Overwintering Ecology Of A Cryptic Migratory Forest Owl, Mitchell Lee Pruitt May 2024

Dispersal Movement And Overwintering Ecology Of A Cryptic Migratory Forest Owl, Mitchell Lee Pruitt

Graduate Theses and Dissertations

Understanding species’ distributions and how a species uses its environment is a vital aspect of ecology and important for the development and implementation of conservation planning. In migratory species, with distinct breeding and nonbreeding ranges, understanding distributions is a complex endeavor. Nevertheless, evaluating movement behavior by linking seasonal distributions and identifying source areas is vital for understanding a species’ full annual cycle ecology. Understanding distributions is particularly important for predators, like birds of prey, that can exert top-down effects on community structure and ecosystem function through their influence on prey populations. Among raptors, owls are severely understudied in large part …


Assessing Health Implications Of Monarch Butterfly Populations And Behaviors, Kira Goldman, Kelsey E. Fisher Dr. May 2024

Assessing Health Implications Of Monarch Butterfly Populations And Behaviors, Kira Goldman, Kelsey E. Fisher Dr.

Honors Scholar Theses

Pollinator populations have seen massive declines over the past decade due to anthropogenic activity and climate change posing serious threats to the health of humans and ecosystems (Ulyshen & Horn, 2023). As agents responsible for the production of about 35% of micronutrient rich food crops necessary for adequate nutrition to lead healthful lives and prevent nutrition associated diseases, pollinator decline is a public health concern (Aizen et al., 2009). Monarch butterflies (Danaus plexippus) serve as bio indicators for the health of pollinator populations because of their analogous habitat and food resources shared with many other pollinator species, including bees and …


Understanding The Role Of Minor Intron Splicing In Spermatogenesis, Jade Rosado, Kazumasa Takemoto, Rahul Kanadia May 2024

Understanding The Role Of Minor Intron Splicing In Spermatogenesis, Jade Rosado, Kazumasa Takemoto, Rahul Kanadia

Honors Scholar Theses

The minor spliceosome is composed of the unique small nuclear RNAs (snRNA), U11, U12, U4atac, and U6atac, and is necessary for the splicing of less than 0.5% of introns, termed minor introns. Minor intron containing genes (MIGs) regulate diverse processes, one of which is spermatogenesis. Specifically, 111 testis-specific MIGs have been identified, suggesting that minor splicing is necessary for spermatogenesis. To interrogate the role of minor splicing in spermatogenesis, we conditionally ablated the Rnu11 gene, which encodes for the U11 snRNA, in the developing testes via Stra8-iCre, creating a Rnu11Flx/Flx::Stra8-Cre+ mutant. We found, at postnatal …


The Association Between Aerobic Exercise And Changes In Hdl Quality Induced By Egg Consumption – A Post-Hoc Analysis Of A Randomized, Controlled, Crossover Dietary Intervention Study, Rachael Woodruff, Catherine J. Andersen May 2024

The Association Between Aerobic Exercise And Changes In Hdl Quality Induced By Egg Consumption – A Post-Hoc Analysis Of A Randomized, Controlled, Crossover Dietary Intervention Study, Rachael Woodruff, Catherine J. Andersen

Honors Scholar Theses

Abstract

Objective: Daily intake of whole eggs and aerobic exercise have independently been shown to increase high-density lipoprotein cholesterol (HDL-C) and measures of HDL function, suggesting cardioprotective effects of these lifestyle interventions. However, it is unknown whether exercise volume can synergistically or differentially impact diet responses and egg effects on HDL. Therefore, we evaluated whether exercise volume was associated with distinct effects of consuming different egg-based diets on measures of HDL size, function, and composition.

Methods: Twenty-six healthy men and women completed a 16-week randomized, controlled, crossover intervention study which aimed to investigate the effects of egg-free, egg-white, and whole-egg …


Archaeal Diversity In The Anna's Hummingbird Microbiome, Lauren E. Chance May 2024

Archaeal Diversity In The Anna's Hummingbird Microbiome, Lauren E. Chance

Honors Scholar Theses

The microbial communities that are present in and on vertebrates are collectively called the microbiome. The composition of a microbiome is dependent upon the host, the environment, and evolution. There has been extensive research on the bacterial composition of host-associated microbiomes, however, there has been much less work on the archaeal composition of host-associated microbiomes. Archaea have previously been assumed to primarily exist in extreme environments, but this may not be true and has been influenced by their generally low abundance and methodological difficulties in detection. It is possible they are consistent members of diverse host-associated microbiomes.

Archaea-specific PCR primers …


Investigating Effects Of Co-Culturing With Vibrio Fischeri On Secondary Metabolite Production Of Hawaiian Bobtail Squid Symbionts, Mariam A. Zedan May 2024

Investigating Effects Of Co-Culturing With Vibrio Fischeri On Secondary Metabolite Production Of Hawaiian Bobtail Squid Symbionts, Mariam A. Zedan

Honors Scholar Theses

Microbes are a fruitful source of natural product drug discovery since many of their secondary metabolites show defensive activity and have unique structures. However, extracting and elucidating the structure of those compounds can be challenging in vitro due to particular environmental and nutritional requirements of some bacteria. Those requirements may include proximity to other organisms that the microbes have symbiotic relationships with. Under those conditions, the organism may activate silent biosynthetic gene clusters (BGCs) responsible for production of certain secondary metabolites not typically expressed otherwise. One method of BGC induction is co-culturing with other microbes since this mimics the natural …