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Plasmin In Milk: Activity Measurement, Effect Of Environmental Factors, And Correlation With Milk Coagulation, Eric D. Bastian

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Bovine plasmin activity was measured on H-D-valyl-L-leucyl-L-lysyl-4-nitroanilide by following absorbance changes at 405 nm. Steady-state kinetic parameters V_max, K_m, K_I, and K_I' were estimated. Bovine plasmin is competitively inhibited by casein and has a K_cat of .0158 ΔA₄₀₅/min/nM, K_m of .107 mM substrate, and K_I of .86 mg/ml casein. Bovine plasmin can be measured directly in bovine milk without interference from casein.

A total of 380 milk samples from nineteen Holstein (one herd) and nineteen Jersey (one herd) cows was collected monthly during one lactation period. Samples …

Chemical Evaluation And Biological Vitamin A Activity Of The Major Carotenoids In The Hybrid Carrot Beta Iii, Cynthia M. Schweitzer

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Genetically enhanced high-carotene Beta III hybrid carrot was compared to a commercial carrot cultivar, Nantes Coreless, for carotene content and macronutrient composition. Beta III contained more than twice the total carotene content of Nantes Coreless, with α-carotene/β-carotene ratios higher in Beta III. Total solids from protein, lipids, and carbohydrate were greater in Beta III than in Nantes Coreless. Beta III contained about five times the amount of sucrose and one-fifth the amount of glucose and fructose than Nantes Coreless or reported literature values.

Comparison of the bioavailability of carotenes in Beta III and Nantes Coreless to purified β-carotene and retinyl …

Proteolytic Activity Of Some Milk-Clotting Enzymes On K-Casein And K-Casein Macropeptide, Khalid M. Shammet

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This work reviews studies of bovine K-casein and specifically K-casein macropeptide. Properties of K-casein, its structure and heterogeneity, proteolytic activity of some milk clotting enzymes on K-casein, and K-casein sensitive bonds are discussed. Macropeptides of other species are also presented. The carbohydrate moieties of bovine macropeptide together with their biological and physiological functions are reviewed.

Macropeptides were produced by enzymic hydrolysis from whole casein solution using crystalline chymosin (EC 3.4.23.4). Trichloroacetic acid (final concentrations 2, 8 and 12%) was added after 5, 30 and 60 min of incubation to precipitate protein and inactivate the enzyme. The filtrate was then exhaustively …

A Quantitative Assessment Of Minerals Of Toxicological Importance In Utah Fast Foods, Lisa R. Williams

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

X-ray flourescence (XRF) and atomic absorption spectrophotometry (AAS) measurements for manganese, iron, copper, and zinc were compared for 96 samples of 21 foods from different sources. Correlation coefficients were 0.94 for manganese, 0.99 for iron, 0.93 for copper, and 0.91 for zinc for XRF vs. AAS determinations.

Similiar comparisons were performed on 228 samples of fast foods purchased in Utah retail outlets. Correlation coefficients ranged from 0.91 for copper to 0.97 for iron and zinc. Comparisons of values generated by XRF for manganese, iron, copper, zinc, selenium, arsenic, and aluminum to values certified by the National Bureau of Standards indicated …

Selenium And Iron In The Rat Intestine: Effects On Lipid Peroxidation, Sileny Vega

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Effects of Fe and Se status on GSHPx activity and lipid peroxidation in liver and intestinal mucosa were studied. Rats were Se and Fe supplemented (+Se+Fe), Se-deficient and Fe-supplemented (-Se+Fe), Se supplemented and Fe overloaded (+Se++Fe) by intramuscular injection, or Se deficient and Fe overloaded (-Se++Fe) for 20d. Fe overloaded tissues had more Fe, but hemoglobin was unaffected. Liver and mucosal GSHPx activity was low in Se-deficient rats. Thiobarbituric acid reactive substances (TBARS) were higher in Fe overloaded and -Se++Fe vs +Se++Fe tissues. Mucosal TBARS was higher in -Se++Fe rats gavaged with CBrCl₃. In experiment 2, Fe overload …

A Longitudinal Study Of Dietary Intake And Influence Of Iron Status On Infants' And Toddlers' Mental And Motor Development, Steven Matley Wood

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Thirty-two non-anemic children were assessed clinically, biochemically and anthropometrically at six months, one year and two years of age to investigate the relationship of iron status to mental and motor development. Using dietary history and dietary record instruments, dietary iron was estimated and compared to the National Food Consumption Survey (1985) and the Recommended Dietary Allowances (RDA) for corresponding age groups. The Bayley Scales of Infant Development (which assess mental and motor development) and the Caldwell Home Inventory (which evaluates the environmental stimuli) were performed and correlated with blood iron parameters, anthropometric measurements and dietary nutrient intakes.

Nutrient intakes of …

Production Of Monoclonal Antibodies Specific For The Gamonts Of Eimeria Tenella, Nancy Carol Larsen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cecal coccidiosis, caused by the protozoan Eimeria tenella, may manifest as a devastating disease in young chickens and result in substantial economic loss for producers. The parasite progresses through a complex life cycle, exhibiting both asexual and sexual (gamont) stages of development. The purpose of this study was to produce a panel of monoclonal antibodies (MoAbs) against epitopes contained on surface antigens (Ags) of the gamonts of E. tenella with the intent of blocking the fertilization process. Gamonts were harvested from infected ceca, partially purified by differential centrifugation throught a discontinuous 5050% Percoll density gradient and used as a …

Utilization Of Poultry Waste (Composted Caged-Layer) As A Supplement For Sheep Fed Straw During Late Pregnancy, Raul Meneses R.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The utilization of composed caged-layer waste (CCLW) in diets for pregnant ewes fed cereal straw was evaluated. Five sheep were assigned to five diets containing, 0, 5.72, 11.37, 23.80, and 32.69% CCLW in a 5x5 Latin square design. Water intake, feed intake and feces output were measured. Feed and feces were analyzed for dry matter, crude protein, acid detergent fiber (ADF), neutral detergent fiber (NDF), organic matter, hemicellulose and minerals. Thus, nutrient digestibility and nitrogen balance could be calculated. Forty dry, pregnant ewes in the last third of generation were assigned to four dietary treatments contained 0, 11.25, 22.08 and …

Winter Habitats And Foods Of Blue Grouse In The Sheeprock Mountains, Utah, Peter J. Perkins, Frederick G. Lindzey, Jay A. Roberson, Gregory Mcdaniel, Randy Berger

Great Basin Naturalist

Winter habitat use and food habits of Blue Grouse (Dendragapus obscurus) were studied in an isolated Utah desert mountain range that contained little typical Douglas-fir (Pseudotsuga menziesii) winter habitat. Habitat use was concentrated in the Douglas-fir and pinyon (Pinus edulis)–juniper (Juniperus spp.) habitat. Douglas-fir and pinyon pine were the most consumed foods. Other foods that represented >15% of the composition of an individual fecal sample were limber pine (Pinus flexilis), mahogany (Cercocarpus ledifoliu), juniper, and an Anteunaria–Cirsium type. The breadth in winter diet indicates that Blue Grouse may …

Morphological Effects Of Lonidamine On Two Human-Tumor Cell Culture Lines, Joseph G. Szekely, Anya U. Lobreau, Sabine Delaney, G. Peter Raaphorst, Mark Feeley

Scanning Microscopy

Lonidamine, 1-(2-4-dichlorobenzyl)-1-H-indazol-3-carboxylic acid. is an anticancer drug that has its primary action on cellular metabolism rather than cell division. Since lonidamine is not effective in all tumor cells, we have tested it in two human-tumor cell culture lines: MOLT-4, a T-leukemia and U-87 MG. a glioma. Lonidamine exposure of MOLT-4 cells at 50 μg/mL and pH 6.7 disrupted the mitochondria within 1 h of treatment. The mitochondria were swollen and the cristae were disrupted. When the treated cells were re-incubated in fresh medium at pH 7.4 the mitochondria rapidly returned to their normal morphology. The U-87 MG glioma cells did …

Salinity Tolerance In Plantago Species: An Ultrastructural And X-Ray Microanalytical Investigation Of Differences In Ion Transport In The Roots, Diana M. R. Harvey

Scanning Microscopy

Root ultrastructure and elemental distributions in regions 3-4 mm behind the apex were examined by transmission electron microscopy and x-ray microanalysis in Plantago maritima ( grown ± 200 mM NaCl) and Plantago media (grown± 20 mM NaCl). In Plantago media vacuolation was incomplete in the cortical and xylem parenchyma cells. Epidermal transfer cells were observed in plants grown in the presence of NaCl. In Plantago maritima grown in the presence of NaCl, vacuolation was complete. In the xylem parenchyma cells of Plantago maritima controls the mitochondria were clustered adjacent to the vessels, rather than evenly distributed throughout the cytoplasm. X-ray …

South Dakota Farm And Home Research, Special Edition: Biostress Laboratory, South Dakota State University

South Dakota Farm and Home Research: 1949 -1998

Director"s comments [p] 2
The opportunity: This "land of infinite variety" give us our opportunity. We must seize it. [p] 3
The NPBL idea: We can 'bring a new focus and a new intensity' to ag research [p] 4
When it's winter in the feed lot: Animal scientists plan 'most intensive' work on cold stress in U.S. for NPBL [p] 7
Of mice and the rest of life: To learn about stress, study a mouse. Then extrapolate what you learn to us [p] 9
New strength in the 'basics': Discoveries in biology are the basis of progress in all agricultural …

Bulletin 4152 - Sheep In Western Australia, R J. Suiter

Bulletins 4000 -

Western Australia's sheep industry started in the early 1830s when the first flock-owners grazed their sheep on extensive areas of native vegetation, often by shepherding on unfenced country. Later, farm flocks built up as settlers cleared and fenced land for cultivation, and 'pastoral' or 'station' sheep were confined to fenced areas of native vegetation, leased from the Government.

By 1900, more than 75 per cent of Western Australia's sheep were still classed as 'pastoral', but by 1987/88, the pastoral areas carried only 2 million of the 34.1 million State flock.

Today, many former sheep stations carry cattle, which survive better …

Population Variation In The Mitochondrial-Dna Of Two Marine Organisms: The Hard Shell Clam Mercenaria Spp. And The Killifish, Fundulus Heteroclitus, Bonnie Lynn Brown

OES Theses and Dissertations

The two major topics are covered in this dissertation: the integration of molecular genetic tools with applied aquacultural research and short-term evolutionary dynamics. The first study investigated the extent of geographic differentiation of native clam stocks along the U.S. east and Gulf coasts. Clam mitochondrial DNA (mtDNA) demonstrated size polymorphism (16.5-19.0 kb) and restriction site heteroplasmy. Size heteroplasmic individuals occurred at a frequencies of 0-89% in clam populations. Restriction site heteroplasmy occurred in 12% of the 317 individuals assayed. Results of the study indicated that although harbouring a wealth of genetic variation, clams from northern regions were similar and lacked …

The Effects Of Potassium Cations On The Germination Of Phytolacca Americana, Karen Michelle Smith

Honors Theses

Phytolacca americana, a plant species of eastern North America, is widely known as a plant of multiple uses in traditional medicine, a source of contemporary research compounds, and as a pot herb when correctly prepared for human consumption. Experimental conditions that promote rapid laboratory germination and the role of chemical factors in the germination process are being investigated in P. americana. The role of potassium ions in plant metabolism, especially the process of seed germination, is reviewed. Experiments were designed, the results of which are presented and discussed, to determine (1) the effect of exogenously applied salts of K+ on …

Agricultural Experiment Station News March 1989

Agricultural Research Division: News and Annual Reports

CONTENTS:
DR. JULIE ALBRECHT
DR. LAURIE HODGES
DR. NANCY LEWIS
DR. DALE WEBB
DR. JEFFREY S. ROYER
DR. DONALD WEEKS
THE STATE AGRICULTURAL EXPERIMENT STATION SYSTEM
DR. JOHN E. FOSTER
PREPROPOSALS - LISA 1990 FUNDING
FY 1991 PRESIDENT'S BUDGET REQUEST
ARD OUT-OF-STATE TRAVEL POLICY PROFESSIONAL SOCIETY MEETINGS
TEKTRAN DATA BASE
ORGANIZATIONS THAT SUPPORT AGRICULTURAL RESEARCH IN LAND GRANT UNIVERSITIES
THE NATIONAL RESEARCH INITIATIVE
1990 ESCOP RESEARCH INITIATIVE PRIORITIES
RESEARCH GRANTS AND CONTRACTS RECEIVED DECEMBER-JANUARY-FEBRUARY
NEW OR REVISED PROJECTS

Effect Of Cyclosporine On The Rate Of Renal Function Recovery After Renal Transplantation, Francis Dumler

Henry Ford Hospital Medical Journal

To assess the effect of cyclosporine therapy on the rate of renal function recovery after renal transplantation, patients with no clinical evidence of rejection and who were treated with either cyclosporine or azathioprine in addition to steroid therapy were studied (n = 74). Of the patients with immediate renal function (n = 57), those receiving organs from living, related donors had a faster recovery rate of glomerular filtration than patients with cadaveric grafts (azathioprine, 15 ± 2 versus 7 ± 1 mL/min/day, P = 0.0001; cyclosporine, 14 ± 3 versus 6 ± 1 mL/min/day, P = 0.001). Recipients of cadaveric …

Back Matter

Henry Ford Hospital Medical Journal

No abstract provided.

Diversity Of Coprophagous Scarabaeidae (Coleoptera) In Grazed Versus Ungrazed Sandhills Prairie In Western Nebraska, Mary Liz Jameson

University of Nebraska State Museum: Entomology Papers

Baited pitfall traps were used to compare the dung-feeding Scarabaeidae (Coleoptera) of a grazed versus an ungrazed sandhills prairie in western Nebraska. Traps yielded 14,832 coprophagous Scarabaeidae over a 4-week sampling period in 1987. Canthon ebenus (Say), C. pilurarius (L.), Onthophagous hecate Panzer, and O. pennsylvanicus Harold were the dominant species on both prairies. The Shannon-Wiener diversity index indicated that diversity was slightly higher on the grazed site, whereas the Margalef diversity index showed the diversities to be nearly the same. Activity and foraging by scarabs (as measured by trapping success) were found to be most closely correlated with temperature …

Wpa News 23 (1989), World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Newsletters

WPA News (February 1989), number 23

Published by the World Pheasant Association

Changes In Mule Deer Size In Utah, Dennis D. Austin, Robert A. Riggs, Philip J. Urness, David L. Turner, John F. Kimball

Great Basin Naturalist

Trends in age-specific, eviscerated carcass weights were determined for hunter-harvested yearling and two-year-old buck mule deer. Carcass weights declined over an 11-year period from two areas of similar management, but with independenly collected data sets. Carcass weights also declined between the opening and second weekends of the hunt. Management implications are discussed.

Comparison Of Yearling Steer Gains In Early Summer Under Season-Long Native, Season-Long Crested Wheatgrass And June-Deferred Native Grazing Systems, P.S. Johnson, J. J. Wagner, M. J. Goetz, J. Cantrell

South Dakota Beef Report, 1989

Season-long native, season-long crested wheatgrass and June-deferred native grazing systems were compared with regard to average daily gains, gains per acre and total gains. Due to drought conditions, the typical 4-month spring-summer season was reduced to a season of approximately 2 months beginning in June. Few differences were detected between the systems for ADG in each month or over the season. Gains per acre were greatest (P<.05) on crested wheatgrass pastures (33.75 Ib/acre), but no differences were detected between native pastures (1 0.61 -1 6.61 Ib/acre) regardless of whether they had been grazed for 2 months or one (using similar stocking rates). This study indicates that, using weight gain data from 1989 and a 2-month grazing season, the greatest potential gain for the season is realized with a system where cattle graze crested wheatgrass in June and native pastures in July. If this system was applied on 320 acres crested wheatgrass and 640 acres excellent condition native pasture, it would have the potential to produce approximately 23,196 Ib of gain on yearling steers compared with season-long (June-July) production of 9,408 Ib on @I0 acres and 14,112 Ib on 960 acres native pasture.

Effects Of Restricting Intake On Carcass Traits Of Young Steers, R. H. Pritchard, D. H. Gee, M. R. Robbins

South Dakota Beef Report, 1989

Feedlot performance and carcass characteristics were compared when steer calves were fed energy dense diets ad libitum (AL) or restricted (RI) to achieve constant growth rates. AL steers grew more rapidly (P.e.10) than RI steers, 2.93 vs 2.74 Ib per head per day. For the period from 98 days to slaughter (AL = 89 days, RI = 99 days), AL steers consumed more dry matter daily than Rl steers. Restricting intake did not affect feed conversions or total dry matter consumed while in the feedlot. Steers were slaughtered at similar final weights. Intake level did not affect carcass weight, rib …

Estrous Synchronization Of Heifers Using Mga And Prostaglandin: Ranch Results, T. B. Goehring

South Dakota Beef Report, 1989

total of 315 yearling heifers were exposed to an estrous synchronization program which consisted of feeding MGA for 14 consecutive days (.5 mg per head per day), followed by a prostaglandin injection 17 days after the last day of MGA feeding. Two hundred forty-seven (78%) of the heifers were detected in heat and artifically inseminated within 5 days after the prostagllandin injection. Response rate ranged from 75 to 84% between ranches and years.

Comparison Of Melengestrol Acetate And Prostaglandin With Two Injections Of Prostaglandin For Estrous Synchronization In Beef Heifers, H. L. Miller, J. J. Wagner, R. L. Hanson

South Dakota Beef Report, 1989

Crossbred beef heifers were used to compare conception rate when synchronizing estrus with melengestrol acetate (MGA) and prostaglandin or two injections of prostaglandin. Melengestrol acetate was fed to one group of heifers for 16 days and prostaglandin injected 16 days after MGA removal or two injections of prostaglandin given 11 days apart in the other group. The MGA group had a higher conception rate (Pc.05) to timed Al, but there was no difference between the two groups for the breeding season. It appears MGA with prostaglandin results in increased conception to a timed insemination.

Evaluation Of Controlled Release Chromic Oxide Boluses And Alkaline Peroxide Lignin As Marker Methods To Determine Forage Intake Of Grazing Ruminants, P.A. Momont, R. J. Pruitt, R. H. Pritchard

South Dakota Beef Report, 1989

Twenty Hampshire ram lambs used in a digestibility trial were administered controlled release chromic oxide intraruminal boluses to evaluate chromic oxide and alkaline peroxide lignin in combination as potential markers for determining intake of ruminants grazing dormant winter range. Lambs were fed ad libitum mature prairie grass hay and provided .1 Ib crude protein from one of four supplements. The ability to predict fecal output using chromic oxide boluses and diet digestibility using alkaline peroxide lignin were not affected by supplemental treatments. Chromic oxide concentration in the feces was not affected by the time of sampling. The amount of chromium …

Moderate Versus High Protein Diets For Finishing Yearling Steers, J.J. Wagner, R. Hansen

South Dakota Beef Report, 1989

Sixty-four yearling crossbred steers (864 Ib) were utilized to study moderate versus high protein finishing diets. Diets were formulated to contain 11.25 or 12.25% crude protein on a dry matter basis. Differences observed for all performance and carcass traits were not significant. Steers on the 11.25 and 12.25% crude protein diets consumed 22.29 and 22.26 Ib dry matter per head daily, gained 2.83 and 2.90 Ib per head daily and required 7.91 and 7.69 Ib dry matter per pound of gain, respectively. Diets formulated to contain 11.25% crude protein appear adequate for finishing heavy yearlings.

Soluble And Insoluble Dietary Fiber In Cooked Common Bean (Phaseolus Vulgaris) Seeds, Joe S. Hughes, Barry G. Swanson

Food Structure

The common bean (Phaseolus vulgaris) requires cooking for extended periods of time prior to consumption. In this investigation both quantitative and microstructural changes in common bean dietary fiber as a result of cooking were examined. Cooking resulted in a slight decrease in soluble dietary fiber and a marked increase in insoluble dietary fiber. The increase in insoluble dietary fiber was responsible for a 15 -30 percent increase in total dietary fiber.

Scanning electron microscopy was used to examine the microstructure of uncooked and cooked bean flours and the insoluble and soluble dietary fiber fractions of these two fl ours. In …

Increasing Profit On Dairy Farms, G R. Olney, Warren Standing

Journal of the Department of Agriculture, Western Australia, Series 4

Farmers continually make decisions on all aspects of their farms that influence profit. For dairy farmers these include the number of cows, calving pattern, level and type of supplementary feeding, area of each pasture type and fodder crop, and the areas for hay or silage, or both. The extent and type of the beef sideline operations also influence the overall profitability of the farm.

The WesternAustralian Dairy Farm Model (WADFM) is a whole farm model developed by the Western Australian Department of Agriculture to help farmers maximise profits by considering all effects of such decisions on the farm.

Potential For Biological Control Of Dendroctonus And Ips Bark Beetles, David Kulhavy, Mitchel C. Miller

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