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Nitrogen Management Strategies For Specialty Tea Production In Louisiana: Rate, Source Type, And Nutrient Dynamics, Autumn D. Hilderbrand

LSU Master's Theses

Tea (Camellia sinensis L.) is an emerging specialty crop in Louisiana and the southeastern United States; however, region-specific nitrogen (N) management recommendations remain limited. This research evaluated N fertilizer source, application rate, and nutrient leaching to identify management strategies that improve nitrogen use efficiency (NUE) and environmental sustainability during tea establishment.

Three complementary studies evaluated N source replacement, application rate, and fertilizer source effects on nutrient retention and movement. The first study evaluated the replacement of urea ammonium sulfate (UAS) with blood meal (BM) while maintaining a constant annual N application rate. Fertilizer replacement did not significantly affect leaf …

Deciphering The Potential Of Monopterus Albus Protein Hydrolysate As A Therapeutic Candidate Against Cervical Cancer By Integrating Content Analysis, Network Pharmacology, And Molecular Docking, Okid Parama Astirin, Widya Mega Rahmawati, Elisa Herawati, Tetri Widiyani, Lili Pandan Sari, Sherly Octaviana

Karbala International Journal of Modern Science

Cervical cancer has a high incidence rate in women, making the development of cancer drugs increasingly urgent. Asian swamp eel protein hydrolysate shows great promise as an anticancer candidate. This study aimed to reveal the potential of Asian swamp eel protein hydrolysate as an anticancer candidate in the cervix. Asian swamp eel protein hydrolysate was produced by hydrolyzing flesh with the enzyme alcalase. Compounds and peptides were identified using LC-HRMS. These compounds and peptides were analyzed using network pharmacology, gene ontology, and molecular docking. The results identified 153 endogenous compounds, including fatty acids and peptides. The key proteins targeted by …

A Comparative Expression Analysis Of Selected Genes Involved In Secondary Metabolites Biosynthesis Among Three Alfalfa Cultivars Grown In Iraq, Izdehar Yahia Obaid, Bushra M. J. Alwash

Al-Bahir

Because of its abundance in proteins, vital amino acids, vitamins, and minerals that promote balanced nutrition, Medicago sativa L., also known as alfalfa, is regarded as a nutritionally excellent forage crop. In addition to its nutritional value, alfalfa produces a range of bioactive secondary metabolites, including flavonoids, alkaloids, and phenolic acids, further supporting its use in medicinal and nutritional applications. Three alfalfa cultivars grown in Iraq were examined for gene expression levels of the primary genes involved in secondary metabolite biosynthesis (PAL, COMT, CHS, and OMT). Among these cultivars are Victoria (VC), Green Gold (GC), and a local cultivar (LC). …

From Insight To Impact: Segmenting Consumers By Their Regenerative Agriculture Priorities, Lourival Carmo Monaco Neto, Gustavo Stevanin De Souza, Owen Jerome Zakrzewski, Allan Wayne Gray

Department of Agricultural Economics Faculty Publications

Regenerative agriculture is gaining traction in agrifood value chains, yet its definitional ambiguity and the heterogeneity of consumer priorities create strategic challenges for commercialization. Firms commonly assume a homogeneous ‘sustainable consumer’ motivated by environmental outcomes, but research on consumer food values suggests that economic attributes often outweigh environmental concerns when trade-offs are required. This study segments U.S. consumers based on how they prioritize seven core attributes of regenerative agriculture: soil health, biodiversity, greenhouse gas emissions, water use, food prices, crop yield, and farmer profitability. Using forced-ranking data from 1231 nationally representative U.S. consumers surveyed in August 2024, we apply a …

Harmful Algal Blooms: Risks For Food In Utah, Jose Brandao, Sarah Erwin, Stephanie Vaughn, Clare Entwistle

All Current Publications

Harmful algal blooms (HABs) are a problem because they can produce toxins that make water unsafe for people, animals, and the environment. In recent years, HABs have emerged as a growing concern in freshwater systems throughout Utah, including irrigation canals, reservoirs, and livestock ponds. HABs in freshwater are caused by cyanobacteria, also called blue-green algae. These microorganisms can release dangerous toxins that can harm swimmers, pets, and the food supply. Unlike foodborne pathogens, these toxins cannot be removed by boiling, cooking, or washing the food. Therefore, regular monitoring is essential to detect HABs early and reduce exposure risks. This fact …

Determinants Of Home Composting Behavior In Supporting Sustainable Agriculture Among Smallholder Farmers: A Case Study In The Thai Nguyen Province Of Vietnam, Bac Ho Van, Hien Vu Thi, Xinh Ho Luong

Journal of Environmental Science and Sustainable Development

Home composting is a practical and sustainable approach for the management of organic waste generated in the home and agricultural production that contributes to both organic waste management and soil fertility enhancement. In the northern mountainous regions of Vietnam, smallholder farmers face limited adoption of sustainable waste management practices due to behavioral constraints, limited technical knowledge, and resource constraints affecting agricultural productivity.  This study applies and extends the Theory of Planned Behavior (TPB) by integrating behavioral and contextual determinants to examine home composting adoption among smallholder farmers in rural Vietnam. Principal Component Analysis (PCA) was used to reduce and structure …

Sophisticated Song: Exploring The Evolution And Ontogeny Of Large Repertoires And Syntax Usage In The Bachman’S Sparrow (Peucaea Aestivalis), Heather Jean Wolverton

Electronic Theses and Dissertations 2020 - Present

While humans have the most complex vocal communication system, many of the features that make human language so intricate can be found in other taxa. One building block of language commonly studied in animal communication is syntax, or the organization of vocalizations. Birds, wolves, and cetaceans all display complex vocal signaling that utilizes syntax. Understanding how syntax functions in different taxa can help illuminate the evolution and development of human language and the role it plays in social cognition. The Bachman’s sparrow (Peucaea aestivalis) is a species of songbird that utilizes syntax in the form of preferred transitions …

Food Systems Transformation Would Reshape Global Agriculture, Matthew Gibson, Marina Sundiang, Daniel Mason-D’Croz, Thais Diniz Oliveira, Felicitas Beier, Lauren Benavidez-Brouk, Astrid Bos, Maksym Chepeliev, Jonathan Doelman, Shahnila Dunston, Shinichiro Fujimori, Tomoko Hasegawa, Petr Havlik, Jordan Hristov, Jonas Jägermeyr, Marta Kozicka, Marijke Kuiper, Page Kyle, Thijs De Lange, Benjamin Leon Bodirsky, Hermann Lotze-Campen, Hermen Luchtenbelt, David Meng-Chuen Chen, Abhijeet Mishra, Christoph Müller, Gerald Nelson, Amanda Palazzo, Ignacio Perez Dominguez, Alexander Popp, Ronald Sands, Marco Springman, Elke Stehfest, Timothy B. Sulser, Kiyoshi Takahashi, Gianmaria Tassinari, Ferike Thom, Philip Thornton, Kazuaki Tsuchiya, Willem-Jan Van Zeist, Hans Van Meijl, Dominique Van Der Mensbrugghe, Detlef Van Vuuren, Hannah H.E. Van Zanten, Isabelle Weindl, Keith Wiebe, Xin Zhao, Mario Herrero

Department of Agricultural Economics Faculty Publications

Food systems are a major contributor to exceeding planetary boundaries1,2,3 and poor quality diets are a key mortality risk globally4. Projected population and income growth could exacerbate these challenges5. In response, there are calls for transformation towards healthy and sustainable food systems6,7,8. However, the scale and distribution of the impacts of this transformation on agriculture are underexplored. Here we show that, by 2050, the transformation of food systems towards healthy diets (adoption of the EAT–Lancet reference diet), improved productivity and halving of food waste results in a fundamental restructuring of global agriculture, aspects of which break with historical trends. Scenario …

Aquaponics: Farming Fish And Growing Greens For Grades 9–12, Amy M. Shambach,, Rebecca M. Ward, J. Stuart Carlton, Kwamena K. Quagrainie, Tomas O. Höök

Purdue University Press Books

Aquaponics: Farming Fish and Growing Greens for Grades 9–12 teaches STEM-related course material through aquaponics, a water-based agricultural system that combines aquaculture (fish production) and hydroponics (soilless vegetable production) systems. This curriculum is aligned with Next Generation Science Standards and designed to be used with an onsite aquaponics system. Topics include an introduction to aquaculture, fundamental parts of an aquaponics system, the nitrogen cycle, water quality, population dynamics, our modern food system, and more. The ten lessons presented build on each other and are intended to be delivered in sequential order. Alternatively, lessons may also be utilized as stand-alone units …

Healthcare Access And The Persistent Racial Disparities In Low Birth Weight, Zaheda A. Hussain, Yadanar Moe

DePaul Discoveries

Low birth weight (LBW), defined as less than 2,500 grams, is an influential factor contributing to infant mortality in the United States, with significant racial disparities between Black and White mothers. This study analyzed data from 3,054 Black and White mothers collected from the 2018 National Health Interview Survey to explore the association between access to medical care and LBW within different racial groups. The prevalence of LBW was 8.0%. Overall, mothers without a usual place of medical care had, on average, an 18% higher risk of LBW than those with a usual place (OR = 1.18, 95% CI: 0.80–1.74). …

From Landfill To Ladle: Using Open Lca To Calculate Food Diversion Impacts, Samantha Rodriguez

DePaul Discoveries

Food waste contributes to climate change because discarded food results in wasting embodied carbon from producing food. In addition to wasting embodied carbon, food that ends up in landfills emits methane, a powerful greenhouse gas that contributes to climate change. This life cycle assessment quantifies the carbon equivalent footprint of food donations from Costco to the Seton Soup Kitchen using OpenLCA. Two functional units were analyzed in this study. The first functional unit is one meal consisting of 500 grams of donated food that would otherwise be landfilled served to a Seton Soup Kitchen patron during one meal service. The …

Fish Tissue Digestion And Microplastic Polymer Identification Protocol Using Open-Source Ftir Database, Shawn L. Kissinger, Vick-Ariel Privert, Jason Bystriansky, Kyle A. Grice

DePaul Discoveries

Microplastic (MP) contamination in aquatic ecosystems poses significant concerns for environmental and public health, as seafood represents a critical food source worldwide. Despite increasing evidence of MP presence in marine organisms, efficient and accessible detection methods remain essential for characterizing contamination patterns and informing regulatory frameworks. This study extracted MPs from brain, gill, intestine, liver, and muscle tissues of gilt-head bream (Sparus aurata) and European sea bass (Dicentrarchus labrax), two commercially important species from the Gulf of Cádiz, Spain, using a NaOH–HNO₃ chemical digestion protocol. Isolated particles were characterized by Fourier Transform Infrared (FTIR) spectroscopy, with …

Campus Food Recovery: Balancing Sustainability And Student Needs, Scott T. Taylor Jr., Wenhao Zhang, Robin B. Dipietro, Mary Stein, Junehee Kwon

ICHRIE Research Reports

The purpose of this study was to assess the impact of a food recovery program at a large university on food waste reduction and food insecurity mitigation. The primary goal of the food recovery program was two-fold: to help provide ready-to-eat meals for students facing food insecurity and to help the campus food service provider minimize post-production food waste. Thus, food was recovered from multiple dining outlets on a large university campus in the southeast United States. To ensure a valid comparison between 2022-2023 and 2023-2024 academic years, a 3-month period during the spring semesters in 2023 and 2024 was …

Effect Of Extraction Methods On Emulsifying Properties Of Corn Fiber Gum, Wu Chenglong, Ren Ying, Li Xingguo, Tang Leiming, Sun Xiaohong

Food and Machinery

[Objective] To investigate the influence of three extraction methods (alkaline, enzymatic, and acidic) on the emulsification characteristics of corn fiber gum (CFG). [Methods] Corn bran was used as the raw material, and CFG was extracted using sodium hydroxide, xylanase, and formic–acetic acid–water solutions. The changes in particle size, zeta potential, rheological properties, creaming index, appearance, and microstructure of CFG emulsions were measured. [Results] All three CFG emulsions exhibited shear-thinning behavior, with viscosities decreasing as the shear rate increased. The enzymatically extracted CFG emulsion had the highest viscosity, best gel properties, smallest particle size [(751.6 ± 22.5) nm], highest absolute zeta …

Strategy For Linear Range Broadening Of Elisa Based On Antibody Synergistic Combination, Guo Cuili, Peng Juan, Lai Weihua, Xing Keyu, Gan Bei

Food and Machinery

[Objective] To broaden the linear range of ELISA for deoxynivalenol detection. [Methods] This paper obtains three antibodies with different sensitivities via mouse immunization, cell fusion and screening, ascites preparation, and antibody purification. By optimizing the working concentration and composition ratio of the antibody synergistic combination, the optimal antibody synergistic combination ratio is applied to ELISA to determine the linear range. [Results] The optimal reaction conditions for the ic-ELISA based on synergistic antibodies are as follows: the optimal working concentrations of the coating antigen and antibody are 0.3 and 0.1 μg/mL, respectively. The linear range of the synergistic antibody is obviously …

Effect Of Double-Bin Plansifter Motion Parameters On Sieving Efficiency, Li Shuang, Wang Fengcheng, Deng Xiangzhi, Li Xinze, Zhang Baowei, Fu Yunfeng, Xue Chunzhi

Food and Machinery

[Objective] The aim is to explore the effect of double-bin plansifter motion parameters on the material sieving efficiency of different systems after wheat milling. [Methods] The milled materials produced by the break system, tailing roll, and scratch system, obtained from a typical milling production line, were selected as raw materials. The double-bin plansifter was used to sieve different materials to investigate the variations in residence time inside the sieve body and in sieving performance under different motion parameters of milled materials in each system. The motion parameters with the optimal sieving performance for the milled materials from each system were …

Extraction Optimization For Polyphenols From Clausena Anisum-Olens Kernel , As Well As Their Antioxidant And Antibacterial Activities, Liao Jianhong, Su Xiufang, Zheng Guangjin, Li Ganghui

Food and Machinery

[Objective] To optimize the extraction process of polyphenols from Clausena anisum-olens kernel, and to analyze their in vitro antioxidant and antibacterial activities. [Methods] With ultrasonic temperature, power, time, as well as solid–liquid ratio and ethanol volume fraction as experimental factors, the response surface method was employed to optimize the ultrasonic extraction process of polyphenols from C. anisum-olens kernel. Furthermore, the in vitro antioxidant activity of the polyphenols was investigated via free radical scavenging assays, and the antibacterial activity was determined using the well diffusion method. [Results] The optimal extraction process is an ultrasonic power of 94 W, an ultrasonic time …

Ameliorative Effects And Mechanisms Of Crocetin On Insulin Resistance In High-Fat Diet-Induced Diabetic Mice, Wen Jing, Qin Si, Lyu Chenghao

Food and Machinery

[Objective] To investigate the hypoglycemic, lipid-lowering, anti-inflammatory, and antioxidant effects of crocetin in high-fat diet (HFD)-induced diabetic mice, and to preliminarily explore its regulatory effects on hepatic insulin signaling pathways and protein S-nitrosylation levels. [Methods] An insulin resistance model was established in C57BL/6J mice by HFD feeding. Mice were administered low (5 mg/kg), medium (10 mg/kg), or high (20 mg/kg) doses of crocetin for 8 weeks, with metformin (200 mg/kg) used as a positive control. Fasting blood glucose, oral glucose tolerance, serum insulin, lipid profiles, hepatic oxidative stress, and inflammatory cytokines were measured. Western blot was used to detect phosphorylation …

Study On Structural Characteristics And Immunomodulatory Activity Of Flammulina Velutipes Crude Polysaccharide, Wang Xinnuo, Peng Chujun, Jin Yumeng, Zhang Molan, You Lijun

Food and Machinery

[Objective] To prepare Flammulina velutipes crude polysaccharide (FVP) and evaluate its structural characteristics and immunomodulatory activity. [Methods] FVP was prepared from Flammulina velutipes using hot-water extraction and ethanol precipitation, and its structural characteristics were characterized by combining Fourier transform infrared spectroscopy (FTIR) and high-performance size-exclusion chromatography–multi-angle laser light scattering–refractive index detection (HPSEC–MALLS–RI). The immunomodulatory activity of FVP was evaluated using RAW 264.7 macrophages and a cyclophosphamide-induced immunosuppressed mouse model. [Results] The total sugar content of FVP is (51.39 ± 1.36) g/100 g. Structural characterization results show that FVP has characteristic features of a β-type pyranose ring and exhibits a multi-component …

Comparative Analysis Of Beef Quality From Bashan Region, Wan Xiaohui, Yao Pingbo, Zhang Duoduo, Ma Xiaowen, Tang Youxue, Liu Yongfeng

Food and Machinery

[Objective] To clarify the unique quality of beef from the Bashan region. [Methods] Xizhen cattle beef from the eastern, southern, south-central, central, and northern regions of Zhenba County, Shaanxi Province, is taken as the material to investigate the differences in nutritional quality (moisture, protein, fat, and minerals) and eating quality (color, texture, and flavor). [Results] Zhenba beef has unique qualities, such as high protein and total mineral content, compared with the nutrient composition of beef in the food nutrient composition database published by the National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention. For nutritional quality, …

Comparison And Optimization Of Glycoprotein Extraction Processes For Six Panax Medicinal Herbs Using Ultra-High Pressure Extraction Technology, Chang Fangyu, Zang Hao, Cheng Liye, Han Lusheng, Du Lianyun, Wang Enpeng

Food and Machinery

[Objective] To comprehensively compare and screen glycoprotein preparation processes for six representative medicinal materials from the genus Panax. [Methods] Glycoprotein yield, DPPH radical scavenging capacity, hydroxyl radical scavenging capacity, and Fe2+ chelation inhibition rate were used as evaluation indicators. Glycoprotein extraction rates and antioxidant activities of six medicinal materials, including P. ginseng, P. notoginseng, P. quinquefolius, P. japonicus (T. Nees) C. A. Mey., P. stipuleanatus H. T. Tsai & K. M. Feng, and P. bipinnatifidus Seem, were compared using traditional thermal extraction and ultra-high pressure processing (UHPP). The Box–Behnken response surface method was employed to optimize UHPP parameters for P. …

Optimization Of Probiotic Fermentation Process And Effects Of Drying Methods On The Quality Of Sea Buckthorn Leaves, Dong Xiaoyue, Yun Xueyan, Zhang Lu, Dong Tongliga, Zhao Chenxi, Te Jinwei, Wang Xiukuan, Hao Qian, Miao Meishu, Su Ke

Food and Machinery

[Objective] To investigate the effects of probiotic fermentation conditions and drying methods on the quality of fermented sea buckthorn leaf products. [Methods] Lactiplantibacillus plantarum is used for fermentation. The fermentation process of sea buckthorn leaves is optimized through single-factor experiments combined with response surface methodology. Fermented sea buckthorn leaf extract is prepared under the optimal fermentation conditions and subsequently processed into freeze-dried powder by direct freeze-drying and spray freeze-drying, with or without low-voltage electrostatic field assistance. The effects of different treatments on viable cell counts, flavonoid and polyphenol retention rates, water activity, and in vitro digestion characteristics are evaluated. [Results] …

Effect Of Modified Hemoglobin As A Substitute For Sodium Nitrite On The Quality Of Ham Sausage, Qu Shulei, Ma Chao, Yao Xianqi, Du Dongxu, Wang Wei, Liu Yunguo

Food and Machinery

[Objective] To improve the utilization rate of porcine blood and the safety of ham sausage. [Methods] Under specific conditions, hemoglobin in porcine blood is modified to produce glycosylated nitrosohemoglobin (GN-Hb). Different amounts of GN-Hb are added to replace sodium nitrite in heat-treated ham sausages. The quality characteristics of the sausages are evaluated through texture analysis, nutritional testing, color difference assessment, color acceptability, elasticity, chewiness, and overall acceptability. [Results] When the sodium nitrite dosage is 100 mg/kg and the GN-Hb dosage is 2.5 g/kg, high-temperature sterilized ham sausage containing the new pigment shows significant improvement in color and cohesion compared with …

Extraction Process Optimization Of Camellia Sinensis Flavonoids And Their Antioxidant Activity, Tu Jianqiu, Xu Danhe, Chen Long, Wang Yaxin, Xu Jiahui, Jing Lixiang, Li Jianfang

Food and Machinery

[Objective] To optimize the extraction process of flavonoids from Xinyang Camellia sinensis and to compare the in vitro antioxidant activities of total flavonoids before and after purification. [Methods] Using Xinyang C. sinensis as raw material, a one-factor test is conducted to investigate the effects of ethanol volume fraction, ultrasonic time, ultrasonic power, and material–liquid ratio on the yield of flavonoids. Subsequently, a three-factor, three-level optimization model is established based on the Box–Behnken experimental design. Additionally, a macroporous adsorption resin is utilized for the purification of flavonoid crude extracts. Finally, the in vitro antioxidant activity of the total flavonoids is evaluated …

Progress On The Nutrient Components, Functional Activities, And Utilization Of Dictyophora Rubrovalvata, Zhang Lunqing, Wang Xianxing, Li Qiaoqiao, Xu Cunbin, Wang Yu

Food and Machinery

Dictyophora rubrovalvata, a valuable edible fungus in China, is rich in active components such as polysaccharides, proteins, amino acids, and vitamins, exhibiting significant efficacy in antioxidant, anti-fatigue, antitumor, immunomodulatory, hepatoprotective, and anti-inflammatory activities. This paper systematically reviews recent research progress on the nutritional components, functional activities, and utilization status of D. rubrovalvata, both in China and elsewhere, with an outlook on its future development directions and application prospects.

Consumer Judgment, Choice, And Meaning-Making In Food Packaging Design From The Perspective Of Consumption Alienation, Guo Jingzhi, Xu Ming

Food and Machinery

In the context of contemporary food consumption, food packaging has gradually shifted from a tool for product protection and information transmission to an important cognitive interface that influences consumer perception, judgment, and choice. Existing studies in this field mainly focus on functional optimization, behavioral guidance, and cultural expression, while insufficient attention has been paid to how packaging design affects consumer cognitive processes. This paper introduces the theoretical perspective of consumption alienation and employs literature analysis and comparative case studies to construct a three-layer analytical framework of “judgment–choice–meaning.” Furthermore, it proposes a regression-based analytical pathway centered on comprehensibility, choice support, and …

Sources And Potential Risks Of Micro- And Nanoplastics In Foods, Zhao Xinlong, Li Wei, Zhang Mengling, Xing Liwen, Tian Huafeng

Food and Machinery

Plastics are widely used in food packaging, storage, processing, and other food-related applications, playing a vital role in extending food shelf life, reducing material loss, and promoting food circulation. However, these plastic products that come into direct or indirect contact with foods can release micro- and nanoplastics through various physical and chemical processes. These micro- and nanoplastics can accumulate in the human body through bioaccumulation and other pathways and have become one of the emerging contaminants threatening food safety and human health. This review provides a comprehensive summary of the research progress on the generation pathways, release mechanisms, potential health …

Effects Of Pretreatment Methods On Volatile Compounds And Active Ingredients In Flaxseed Oil, Zhu Lijun, Chen Fengzhen, Zhang Chengshuang, Zhao Baoyi, Meng Lingjun

Food and Machinery

[Objective] This paper aims to investigate the effects of microwave and roasting pretreatment methods on volatile compounds and active ingredients in flaxseed oil. [Methods] Flaxseeds were pretreated by microwave (720 W, 2–8 min) and roasting (140–200 ℃, 30 min), and then flaxseed oil was prepared by cold pressing. Using untreated cold-pressed flaxseed oil as a control, the differences in volatile compounds were analyzed by headspace gas chromatography–ion mobility spectrometry (HS-GC-IMS) and an electronic nose. Additionally, the contents of tocopherols, phytosterols, and total phenolics were determined. [Results] A total of 72 volatile compounds are identified by HS-GC-IMS analysis, including aldehydes (20 …

Advances In The Application Of Electroencephalography In Food Sensory Science, Li Wenbin, Hou Xiaoyi, Deng Na, Leng Tuo, Fu Haohua, Wang Jianhui

Food and Machinery

Traditional sensory evaluation of food is affected by subjectivity and other factors, resulting in significant limitations. Electroencephalography (EEG), owing to its advantages of high temporal resolution, non-invasiveness, and real-time dynamic monitoring, offers an objective neurophysiological perspective and analytical approach to address this issue, effectively advancing food science research from subjective descriptions to objective neural decoding. This paper systematically reviews the applications of EEG in food flavor recognition and prediction, objective evaluation of food quality, and analysis of food nutrition and consumer behavior. In light of the current state of technological development, it is proposed that multimodal technology integration and machine …

Impact Of Social Media-Driven Feeding Practices On The Gut Microbiome Of Captive Asian Elephants (Elephas Maximus) In Surin, Thailand, Pongpreecha Malaluang, Nuttapon Bangkaew, Jaruwan Chaiyakhot, Patinya Patikae, Nattaya Watwiengkam, Phimphanit Choklikitumnuey, Thanaphat Phupanna, Sudachanok Phowiset, Prim Chuesaard, Tarid Purisotayo

The Thai Journal of Veterinary Medicine

This study investigated the effects of social media-driven live-streaming dietary supplementation on the gastrointestinal microbial community of captive Asian elephants (Elephas maximus) at the Elephant Kingdom, Surin Province, Thailand. A hierarchical two-tier observational design was used: Tier 1 compared a minimal-supplementation control group (Group H, n = 6) with a frequent heterogeneous-supplementation group (Group L, n = 18); Tier 2 compared, within Group L, low-frequency (Group A, ≤ 3 sessions per week, n = 7) and high-frequency (Group B, > 3 sessions per week, n = 11) live-streaming sub-groups. Faecal microbial profiles were obtained by 16S rRNA gene (V3–V4) …