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Rab32c Mediates Osmoregulation And Calcium Homeoastasis In Dictyostelium Discoideum, Ashley Esparza

Theses and Dissertations

Vesicular trafficking is a fundamental process essential to cell viability and dictates roles in both cellular and tissue function in multicellular organisms. To further understand various vesicular trafficking processes, our lab focuses on the moderators of these processes, Rab GTPases, using the model organism Dictyostelium discoideum. This dissertation focuses explicitly on the uncharacterized protein Rab32C, a homolog of human Rab32. While several Rab family members have been well defined, the functionality of Rab32C remains poorly understood. This project investigated both the subcellular localization and functional role of Rab32C with biochemical, imaging, and molecular assays. Results from this study provide evidence …

Mapping Climatic And Environmental Determinants Of Parasitic Helminth Prevalence In Southeast Asia, Angelica Avisado

UNLV Theses, Dissertations, Professional Papers, and Capstones

Climate change is reshaping environmental conditions across Southeast Asia (SEA) through rising temperatures, altered precipitation patterns, and increased droughts. These changes alter transmission dynamics of neglected tropical diseases (NTDs) by shifting spatial distribution, increasing larval development rates, faster pathogen growth development, and increasing transmission rates. NTDs are a group of preventable and treatable infections that disproportionately affect more than 1 billion people globally, specifically in tropical and subtropical regions. A subgroup of NTDs, parasitic helminth infections (PHIs) - particularly schistosomiasis, soil-transmitted helminths, and lymphatic filariasis remains a public health concern in the Southeast Asian region. PHIs and their ecological responses …

Fabrication Of Three-Dimensional Seaweed-Based Scaffolds And Evaluation Of Cell Attachment And Viability For Cultured Meat Applications, Srishti Tandon

Honors Scholar Theses

Global demand for animal protein is increasing rapidly, underscoring the need for sustainable meat production. Cultured meat is a promising alternative to traditional meat production techniques, but it requires optimization before it can be implemented on a large scale. Scaffolds are an important component for making cultured meat, as they provide structural support for cell attachment and mimic the texture of meat. Scaffolds need to be biocompatible, mechanically supportive, and biochemically active to promote proper cell adhesion, proliferation, and differentiation. This project investigated brown seaweed extract (SWE) hydrogels as a scaffold for cultivated meat applications and compared their mechanical and …

Applied Research For Corn Production In Indiana, 2023, Daniel Quinn

Purdue University Press Books

The 2023 edition of Applied Research for Corn Production in Indiana offers accessible, research-based tips for farmers who want to improve corn production practices by using applied field-research trials. Daniel Quinn and the Purdue University Corn Agronomy team reveal practical ways to boost yields, reduce costs, and improve sustainability on the farm. Their findings are derived from applied research trials across the state and relate to important topics such as planting practices, fertilizer use, crop nutrition, cover crops, and new technologies. Applied Research for Corn Production in Indiana distills complex research into clear insights and supplies corn producers with effective …

Applied Research For Corn Production In Indiana, 2024, Daniel Quinn

Purdue University Press Books

The 2024 edition of Applied Research for Corn Production in Indiana offers accessible, research-based tips for farmers who want to improve corn production practices by using applied field-research trials. Daniel Quinn and the Purdue University Corn Agronomy team reveal practical ways to boost yields, reduce costs, and improve sustainability on the farm. Their findings are derived from applied research trials across the state and relate to important topics such as planting practices, fertilizer use, crop nutrition, cover crops, and new technologies. Applied Research for Corn Production in Indiana distills complex research into clear insights and supplies corn producers with effective …

Knowledge, Attitudes, And Practices Concerning Water, Sanitation, And Hygiene And Related Diseases Among University Students In Malaysia, Renee Ng Jeffrey, Aida Soraya Shamsuddin, Farah Diyana Ariffin, Aziemah Zulkifli

Makara Journal of Health Research

Background: Water, sanitation, and hygiene (WASH) practices are fundamental components of public health, being crucial in preventing disease transmission and promoting healthy living environments. In this context, university students play an essential role as future professionals and community advocates in fostering proper hygiene behaviors and preventing disease outbreaks. Understanding knowledge, attitudes, and practices (KAP) in regard to WASH among individuals, particularly university students, is crucial for promoting proper hygiene behaviors. This study aimed to assess the KAP in regard to WASH and related diseases among undergraduate students from the Faculty of Health Sciences, Universiti Kebangsaan Malaysia (UKM).

Methods: …

Spinach (Spinacia Oleracea L) Flavonoids Are Hydrolyzed During Digestion And Their Bioaccessibility Is Under Stronger Genetic Control Than Raw Material Content, Michael P Dzakovich, Alvin L Tak, Elaine A Le, Rachel P Dang, Benjamin W Redan, Geoffrey A Dubrow

Children’s Nutrition Research Center Staff Publications

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The Effect Of Phosphate Fertilizer And Organic Matter On Phosphorus Status, Ph, And Cabbage Yield In Andisols, Mahfud Arifin, Emma Trinurani Sofyan, Anni Yuniarti, Rina Devnita, Sastrika Anindita

Jurnal Kultivasi

Andisols are characterized by high phosphorus (P) retention, which often limits P availability for crops. This study aimed to analyze the independent and interactive effects of P fertilizer and organic matter (i.e. manure and rice straw compost) on P retention, total P, available P, P uptake, soil pH, and cabbage yield. This research was conducted in pot experiments in plastic house, applying P fertilizer (0, 90, 180, 270 kg ha-1) and organic matter (20 kg ha-1 of chicken, sheep, cow manures and rice straw compost) on Andisols planted with cabbage. The experiment used a factorial randomized design. The results showed …

Fruit Characteristics Of F1 Nil Sletr1-2 Vary With Parental Background, Imas Mintarsih, Anne Nuraini, Farida, Erni Suminar, Rika Meliansyah, Syariful Mubarok

Jurnal Kultivasi

The Sletr1-2 allele, which reduces ethylene sensitivity in tomatoes, offers a promising approach to extending fruit shelf life by slowing ripening-related metabolism. However, the trait’s success depends on compatibility with the recipient cultivar’s genetic background. This study evaluated fruit characteristics of F1 near-isogenic line (NIL) hybrids carrying Sletr1-2 in the commercial backgrounds ‘Costoluto’, ‘Oxheart’, and ‘Sart Roloise’. The field trial was carried out between May and August 2024 at the Experimental Station of Universitas Padjadjaran, Sumedang, Indonesia, following a randomized complete block design with eight biological replicates for each treatment. The results revealed that fruit characteristics of F1 NIL Sletr1-2 …

Effects Of Supplemental Led Lighting And Biofertilizer On Yield And Fruit Quality Of Japanese Strawberry Cultivars In Indonesia, Reginawanti Hindersah, Febby Fitriyani, Kenji Endo, Masakazu Nakayama, Syariful Mubarok, Gita Bina Nugraha

Jurnal Kultivasi

Growing strawberries in soilless substrates in the greenhouse relies on fertilizers and supplemental light to boost yield and fruit quality. The research evaluated the properties of the biofertilizer and its effectiveness on the yield of Japanese strawberry cultivars Nanatsuboshi and KS-75 under controlled greenhouse conditions, with and without light-emitting diodes (LED) as supplemental light. The cell viability and plant-growth-promoting properties of a liquid consortium biofertilizer consisting of nitrogen-fixing bacteria and phosphate-solubilizing microbes, were characterized. Laboratory analysis confirmed the biofertilizer's composition, and a greenhouse experiment using a completely randomized design tested treatments of biofertilizer, LED, and their combination. The biofertilizer contained …

Development Of An Artificial Intelligence Web Based System For Classification Of Tomatoes Disease, Joshua Sokowonci Mommoh, Etinosa Noma-Osaghe, Vera Nimo Timai, Odike Arnold Uchechi, Solomon Obhenbhen Ibharunujele, Blessing Godday

Mansoura Engineering Journal

The development of this platform addresses the challenges associated with traditional tomato disease detection methods as well as the challenges of current machine learning and deep learning models in accurately detecting tomato diseases. Conventional methods of tomato disease diagnosis typically rely on expert evaluation but are time-consuming and susceptible to human mistakes. Incorrect diagnoses of certain diseases could lead to poor control measures, resulting in decreased agricultural productivity. In this research, the PlantVillage dataset, comprising 18,160 tomato leaf images distributed across 10 classes, was used to train two machine learning models, namely, Inception-ResNetV2 and Inception V3. The models were configured, …

The Relationship Between Light Physical Activity And 10-Year Weight Change In 2,868 U.S. Adults: A National Health And Nutrition Examination Survey Investigation, Chloe Diane Cory

Theses and Dissertations

The prevalence of obesity has steadily increased over the past several decades. About 13.4% of Americans aged 20-74 had obesity in 1960-1962 and the prevalence has steadily increased to 40.3% in 2021-2023--more than a 3-fold increase. PURPOSE: The aim of this study was to evaluate the relationship between time spent in light physical activity (LPA) and 10-year weight change in a random sample of 2868 women and men representative of the U.S. adult population. METHODS: Data were used from the National Health and Nutrition Examination Survey (NHANES) from the 2003-2006 data cycles. Participants were limited to non-pregnant U.S. …

Aqueous Geochemistry Drives Microbial Cu, Fe, And Zn Uptake Rates In Yellowstone, A Continental Hydrothermal System, Eva Carolina Andrade-Barahona

Graduate Theses & Non-Theses

Microbial uptake of trace metal cofactors such as copper (Cu), iron (Fe), and zinc (Zn) is governed by biogeochemical processes in hydrothermal systems where metal bioavailability varies widely. In situ microbial metal uptake rates were quantified by applying metal stable isotope probing (MSIP) in the Yellowstone hydrothermal system across diverse geochemical conditions in hydrothermal springs. The findings in these studies revealed that pH exerts a dominant influence on microbial copper uptake rate, also enhanced by increased Cu²⁺ ion availability and dissolved oxygen. Microbial iron uptake, in contrast, displayed a broad tolerance to environmental variability, occurring across a pH range of …

Variation In Nectar Rewards Under Water Limitation Across Flowering Species, Madeline Isabelle Gregor

Undergraduate Honors Theses

Drought is becoming an increasingly dominant force shaping plant–pollinator interactions, yet we still know little about how nectar rewards respond to water limitation across species commonly used to support pollinators. We examined nectar volume and sucrose content in six flowering species relevant to California’s Mediterranean climate and quantified how drought alters these traits for four of those species. Under well-watered conditions, species differed in their nectar production, spanning more than two orders of magnitude. Perennial species such as Epilobium canum and Scrophularia californica produced the highest nectar volumes and sucrose amounts, suggesting that they contribute substantial carbohydrate resources when moisture …

Determinants Of Farmers’ Choices Of Adaptation Strategies In Rural Semi-Arid Agricultural Communities Of Tanzania: A Case Study Of Mwanga And Same Districts In Kilimanjaro Region, Bahati Ally Magesa

Journal of Humanities and Social Sciences

Climate change is a global phenomenon that poses a significant threat to agricultural farming and the livelihoods of farmers, particularly in semi-arid regions of Sub-Saharan Africa. Since agriculture is the primary source of employment in the region, enhancing the sector’s adaptive capacity has become increasingly important. This paper examines the key determinants influencing farmers’ choices of adaptation strategies to climate change impacts in the Mwanga and Same Districts of Tanzania. A total of 200 farmers participated in a household survey conducted using a simple random sampling technique. A binary logistic regression model was employed to analyse the association between selected …

Effects Of Integrated Biological And Chemical Fertilization On Pecco And Banji Shoot Development In Tea Plants, Intan Ratna Dewi Anjarsari, Muhammad Raihan Abdillah Farros, Santi Rosniawaty

Jurnal Kultivasi

Tea productivity and quality are largely determined by shoot structure, particularly the balance between pecco shoots (tender buds and young leaves) and banji shoots (older, less productive shoots). Conventional reliance on inorganic fertilizers often enhances overall growth but does not necessarily improve shoot structure, while biofertilizers alone provide limited nutrient availability. This study aimed to evaluate the effects of combining microbial-based liquid biofertilizers with inorganic fertilizers on the structure of tea shoots. The experiment was carried out on seed-derived tea plants using a randomized block design with several fertilization treatments, including sole biofertilizer, sole inorganic fertilizer, and their combinations. The …

Heat Increment Of Feeding In The Common Bottlenose Dolphin (Tursiops Truncatus) Contributes Moderately To Field Metabolic Rate Estimates, Ioulia Koliopoulou, Stacy L. De Ruiter, Jordi Altimiras, Josefin Larsson, Julietta Arenarez, David Rosen, Andreas Fahlman

University Faculty Publications and Creative Works

Digestion elevates metabolism through the heat increment of feeding (HIF) – the energy expended on mechanical and biochemical processes after eating. Quantifying this cost is essential for bioenergetic models that predict energy flow and prey requirements in populations. Using breath-by-breath respirometry, we measured oxygen consumption (V̇ O2 ) in eight common bottlenose dolphins (Tursiops truncatus) before and after feeding standardized meals (1659–2658 kcal of capelin and herring). Metabolic rate rose by ∼37% above resting levels, peaking 60 min after feeding before returning to baseline within 2 h. When scaled across the day, digestion increased daily metabolic needs by ∼8.2% of …

Application Of Augmented Reality Technology As A Dietary Monitoring And Control Measure Among Adults: A Systematic Review, Gabrielle Victoria Gonzalez, Bingjing Mao, Ruxin Wang, Wen Liu, Chen Wang, Tung Sung Tseng

School of Public Health Faculty Publications

Background/Objectives: Traditional dietary monitoring methods such as 24 h recalls rely on self-report, leading to recall bias and underreporting. Similarly, dietary control approaches, including portion control and calorie restriction, depend on user accuracy and consistency. Augmented reality (AR) offers a promising alternative for improving dietary monitoring and control by enhancing engagement, feedback accuracy, and user learning. This systematic review aimed to examine how AR technologies are implemented to support dietary monitoring and control and to evaluate their usability and effectiveness among adults. Methods: A systematic search of PubMed, CINAHL, and Embase identified studies published between 2000 and 2025 that evaluated …

Sex Disparities In Prescription Opioids Associated Cardiovascular Adverse Events, Li Ma

Theses and Dissertations

The opioid crisis in the United States has evolved from a medical response to pain into a nationwide public health emergency. The prescription opioids (POs) – associated cardiovascular adverse events (CVAEs) remain insufficiently studied, especially in relation to sex disparities. This dissertation investigates sex disparities in PO-associated cardiovascular adverse events (CVAEs) through an analytic framework integrating artificial-intelligence-assisted literature mining and real-world pharmacovigilance analytics. In the analytic framework, first, AI powered topic modeling was employed to uncover POs-associated CVAEs risks with a focus on sex differences. A total of 1,837 PubMed abstracts were analyzed using two methods, Latent Dirichlet Allocation (LDA) …

The Effects Of Nanobubble Fertigation And Soil Conditioners To Improve Nutrient Dynamics, Soil Microbial Activity, And Chili Productivity, Betty Natalie Fitriatin, Euis Ahah Rokayah, Elsa Naila Elmyra, Tualar Simarmata, Nicky Oktav Fauziah, Hanif Fakhrurroja

Jurnal Kultivasi

Drip fertigation is a fertilization method that integrates nutrient delivery with an irrigation system, which is capable of optimizing the direct delivery of nutrients to the root zone. This experiment aims to investigate the effect of drip fertigation with nanobubble technology, and soil conditioners on the population of Phosphate-Solubilizing Bacteria (PSB) and Azotobacter spp., available phosphorus, total nitrogen, growth, and yield of red chili. The study used a Strip Plot Design with two factors and three replications. The first factor was nutrient application (solid NPK fertilizing as control; drip fertigation; and drip fertigation with nanobubble technology), and the second factor …

Effect Of Nano Edible Coating, Storage Temperature, And Packaging On Water Content, Weight Loss, And Color On Oyster Mushrooms (Pleurotus Ostreatus), Inneke Amalia, Warid Ali Qosim, Kusumiyati

Jurnal Kultivasi

Oyster mushrooms are one of the most commonly consumed mushrooms due to their high nutritional content, including protein, fiber, vitamins, and minerals, as well as their antioxidant properties. However, these mushrooms are easily damaged by microbes and poor post-harvest handling, which affects their quality. The combination of nano-edible coating, packaging, and storage temperature offers a solution to these issues. This study aims to evaluate the effects of nano-edible coating, packaging, and storage temperature on water content, weight loss, and the color (L*, a*, and b*) of oyster mushrooms. The study was conducted at the Horticulture Laboratory, Faculty of Agriculture, Universitas …

Study Of Insect Frass-Associated Microbiome On Plant Growth And Their Potential Use As Biofertilizer, Jasmine Sahota

Theses and Dissertations

An increasing global food demand and the urgent need for sustainable agricultural practices have driven interest in insects as alternative feed and food sources. Insect frass, a by-product of insect rearing, represents a rich organic carbon source with diverse microbes that can enhance soil health and promote beneficial plant-microbe interactions. This study characterized the bacterial (16S-V4) and fungal (ITS2) microbiomes of frass from Black Soldier Fly (BSF) and Cricket species and evaluated their effects on maize (Zea mays) growth. Four frass variants were applied at rates of 56-280 kg ha⁻¹ in a pot experiment with untreated soil and mineral fertilizer …

The Biology Of Green Antelopehorn Milkweed (Asclepias Viridis Walter) And Hemp Dogbane (Apocynum Cannabinum L.) And Response To Triclopyr Formulations And Other Auxin Herbicides, Kayla L. Broster

Theses and Dissertations

Studies were conducted in Oktibbeha and Clay Counties in Mississippi to determine the response of green antelopehorn milkweed and hemp dogbane to triclopyr and other auxin herbicides in non-cropland areas. These studies were conducted at four locations with well established populations for each species throughout 2020 to 2023, with applications repeated at each location the following year if populations persisted. Treatments included a high and low rate of each herbicide studied, with all four triclopyr formulations included for both species. Injury response data were collected two and four weeks after treatment and population density data were evaluated using individual stem …

Trace Mineral Supplementation Strategies For Beef Cattle During Preconditioning And Feedlot Receiving, Madeline G. Mcknight

Theses and Dissertations

Beef cattle routinely undergo a variety of stressors within the preconditioning and feedlot receiving phases which may impair animal performance and health. Trace mineral supplementation, either through dietary or injectable sources, is proposed to mitigate these effects through metabolic and immune system support. Two systematic reviews and meta-analyses were conducted to evaluate the efficacy of two different oral supplementation methods, organic (OTM) and inorganic (INR), and injectable trace mineral (ITM) solutions on average daily gain (ADG) and morbidity. Across 20 studies, OTM supplementation increased ADG (P = 0.01) when compared to INR, but did not affect morbidity (P = 0.92). …

Spatiotemporal Ecology Of Mycobacterium Ulcerans And Mycolactone Functions In French Guiana And Southeastern United States Aquatic Environments, Magdalene Dogbe

Theses and Dissertations

Mycobacterium ulcerans, the causative agent of Buruli ulcer, produces mycolactone, a polyketide toxin traditionally studied for its role in human pathogenesis. This dissertation presents a comprehensive spatiotemporal analysis of M. ulcerans and mycolactone producing mycobacteria (MPM) ecology in the Americas and also reveals mycolactone's role in the environment as a microbial community regulator. Through comparative studies in French Guiana (endemic) and the southeastern United States (non-endemic), combined with experimental microcosm investigations, this research contributes to environmental pathogen ecology understanding. Environmental surveillance across 2,226 samples from French Guiana revealed complete MPM restriction to freshwater environments, with zero detections from 1,226 coastal …

Evaluating The Effects Of Biostimulants On Apple Coloration, Patrick G. Owens

Theses and Dissertations

Apple peel color is one determining factor of apple quality and is a highly sought after trait by both growers and consumers. Historically plant growth regulators (PGRs) such as daminozide and auxins have been used to induce a greater amount of red color in the peel, but these products typically have negative production impacts and raise environmental concerns. One new area of interest is in called biostimulants. This study investigated the effects of two experimental biostimulants (MCEN1 and MCEN2) applied at varying rates and timings, as well as a commercially available plant growth regulator (Blush® 2X), on ‘Honeycrisp’ apple color …

How The Intake Of Pulses May Impact Metabolic Disorders And Dementia Risk: A Narrative Review, Lisa Michelle Belzer Salinas, Maricarmen Marroquin, Mariana Mendez, Isabel Omaña-Guzmán, Juan Carlos López-Alvarenga

Health & Biomedical Sciences Faculty Publications

We present a narrative review focusing on pulses’ geographical origin and distribution, their impact on human evolution and history, and their influence on human health. Pulses, including dry peas, beans, and lentils, are renowned for their richness in chemical antioxidants. Despite containing antinutrients, processing techniques preserve their health advantages. Epidemiological research has consistently demonstrated that the consumption of pulses is associated with favorable effects on metabolism. This evidence is further supported by molecular and clinical research, which has elucidated potential nutrigenomic mechanisms and effects on gut microbiota composition underlying their health benefits. However, the literature lacks randomized controlled clinical trials …

Fitness Changes, Dietary Practices And Sleep Quality In Non-Elite Through-Hikers: A Case Series, Cory J. Greever, Blayke Harrison, Holland Gaston, Anna Grexton, Dante Argento

Topics in Exercise Science and Kinesiology

Topics in Exercise Science and Kinesiology Volume 6: Issue 1, Article 13, 2025. Through-hikes on long distance backpacking routes have the potential to both improve physical fitness and pose health risks. PURPOSE: To quantify changes in cardiorespiratory fitness and body composition, while recording dietary intake and sleep habits in a sample of through-hikers. METHODS: Participants completed two lab visits consisting of a maximal graded treadmill test and body composition assessments. Hiking volume, energy expenditure, sleep duration, and sleep staging were estimated during the hike using a smart watch. Participants kept a journal of all food and beverage intake. RESULTS: Over …

Reducing Foodborne Bacteria Public Health Burden By Novel Compounds And Pressure-Based Technologies, Junice Alannah Sibley

Tennessee State University Alumni Theses and Dissertations

Food safety strategies are being developed in the hopes of combating foodborne pathogens and their risks. Previous techniques and technologies are struggling to meet the needs of the public, leading to the demand for new, innovative methods. Mild high-pressure processing (HPP) of 350 MPa was used in a synergetic combination with mild heat of 60°C and the antimicrobial carvacrol for the decontamination of non-typhoidal Salmonella serovars that resulted in a >5 log CFU/mL reduction. Additionally, a secondary study was conducted comparing a single Salmonella Tennessee strain with a 4-strain Salmonella cocktail to examine sensitivity to pressure-based treatments resulting in a …

Acid And Sequential Acid–Alkali Extraction To Mitigate Enzyme Activity And Off-Flavors In Chickpea Protein: Implications On Structural, Functional, And Bioactive Properties, Kavitha Lakshmipathy, Sukanya Poddar, Nikitha Modupalli, Scott Lafontaine, Md Mahfuzur Rahman

Food Science Faculty Publications and Presentations

Chickpea proteins offer significant nutritional and functional benefits in food products; however, their utilization is often restricted by off-flavors, primarily for lipoxygenase (LOX) and lipase activity. This study aims to develop a novel extraction method by combining acid (pH 2.0) extraction with the conventional alkali (pH 9.0) method to inactivate enzymes and enhance protein quality. Acid-extracted proteins exhibit the highest purity (83.4%), albumin content, and structural flexibility (46.65% random coil). Both acid and sequential acid-alkali-extracted proteins significantly reduced LOX (0.75 and 0.88 U/g) and lipase (0.10 and 0.08 U/g) activity. Acid extraction significantly lowers volatile compounds such as hexanal (0.0409 …