Digital Commons Network^™

Effect Of The Ahr Inhibitor Ch223191 As An Adjunct Treatment For Mammarenavirus Infections, Miguel Angel Pelaez, Jonna B. Westover, Dionna Scharton, Cybele Carina García, Brian B. Gowen

Animal, Dairy, and Veterinary Science Faculty Publications

The family Arenaviridae encompasses zoonotic, rodent-borne pathogens (e.g., Lassa, Machupo, and Junín viruses) that cause severe viral hemorrhagic fevers with high case fatality rates. The current therapeutic landscape is severely limited, underscoring the urgent need for novel antiviral strategies. A promising approach involves combining directly acting antivirals with host-targeted antivirals. A compelling host-targeted antiviral target is the aryl hydrocarbon receptor (AHR). This ubiquitous ligand-activated transcription factor is a recognized pro-viral host factor across multiple viral families. Building on prior work with Junín and Tacaribe viruses, we investigated whether the AHR inhibitor CH223191 could enhance the virus-directed antiviral activity of favipiravir …

Biostimulant Applications In Watermelon Production, Evan A. Christensen, Milena Oliveira, Youping Sun, Grant Cardon, Dan Drost

All Current Publications

Watermelon is a water-intensive vegetable crop that is sensitive to drought and requires adequate nutrient supply for good yield and fruit quality. It is an important vegetable crop grown on 142,000 acres in the United States and 586 acres throughout Utah. Utah experienced its driest year on record in 2020 during a five-year drought (2019 to 2023). This affected local ecosystems, including the Great Salt Lake, which experienced record-low water levels. The drought also negatively affected the agricultural sector, including watermelon growers. Higher-than-average fertilizer prices in recent years have also threatened watermelon production. Growing awareness of agriculture's water use and …

The Effects Of Magnesium Glycinate Vs. Magnesium Citrate On The Growth Of Lactobacillus Acidophilus In Simulated Gastrointestinal Conditions, Inaya Siddique

Journal of the South Carolina Academy of Science

No abstract provided.

Isolasi Yeasts Dari Daging Dan Potensinya Sebagai Agen Biopreservasi Dan Pewarna Makanan (Isolation Of Yeasts From Meat And Its Potential As Bio Preservation Agent And Food Coloring), Wendry Setiyadi Putranto, Roostita L. Balia, Obin Rachmawan, Eka Wulandari

Jurnal Ilmu Ternak Universitas Padjadjaran

Yeasts merupakan mikroba potensial untuk dikembangkan sebagai penghasil komponen bioaktif seperti enzim, antimicrobial agent sebagai biopreservasi, serta pengahasil pewarna makanan (pigment).  Penelitian merupakan studi ekploratif potensi yeasts yang diisolasi dari daging ayam dan sapi. Ditemukan isolate yeasts yang memiliki karakteristik spesifik dengan warna merah.  Jumlah total yeasts pada daging ayam 2.105 cfu/gram sedangkan pada daging sapi 1.105 cfu/gram. Hasil pemurnian isolate  diperoleh dua isolate yang memiliki kemampuan daya hambat yang terhadap Pseudomonas aerugenes dan Staphylococus aureus serta tidak meiliki aktivitas penghambatan terhadap Escherichia coli dan Candida albicans. pH akhir media selama fermentasi 25 0C, 48 jam adalah 5,5 dan 5,6 …

Pemanfaatan Gelatin Tepung Sagu (Metroxylon Sago) Sebagai Bahan Pakan Ternak Ruminansia (Utilization Of Sago (Metroxylon Sago) Gelatin As Feed Ruminant), Harry Triely Uhi

Jurnal Ilmu Ternak Universitas Padjadjaran

Telah dilakukan penelitian pemanfaatan gelatin sagu sebagai bahan pakan ternak ruminansia. Tujuan penelitian untuk mengetahui suhu, waktu dan ratio volume air yang tepat selama pemanasan sampai terbentuknya gelatinisasi. Justifikasi terjadinya proses gelatinisasi didasarkan atas rusaknya bentuk granula yang dilihat dengan  mikroskop elektron dan mikroskop polarisasi. Perlakuan yang digunakan R1 (Tepung sagu 100 gram + suhu   80 ⁰C); R2 (Tepung sagu 100 gram + suhu   85 ⁰C); R3 (Tepung sagu 100 gram + suhu   90 ⁰C); R4 (Tepung sagu 100 gram + suhu   95 ⁰C); dan R5 (Tepung sagu 100 gram + suhu 100 ⁰C). Parameter …

Pengaruh Lama Penyimpanan Semen Cair Ayam Buras Pada Suhu 5 0c Terhadap Periode Fertil Dan Fertilitas Sperma (The Storage Time Effect Of The Local Chicken Chilled Semen At 5 0c On Fertility And Fertile Period Of Sperm), Nurcholidah Solihati, Ruhijat Idi, Rangga Setiawan, I.Y. Asmara

Jurnal Ilmu Ternak Universitas Padjadjaran

Tujuan penelitian ini untuk mengetahui sejauh mana pengaruh lama penyimpanan semen cair ayam buras pada suhu 5⁰C terhadap periode fertil dan fertilitas sperma.  Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan setiap perlakuan diulang 6 kali.  Lama penyimpanan yang digunakan dalam penelitian ini adalah penyimpanan 1 jam (0 hari), 24 jam (1 hari) dan 48 jam (2 hari).  Peubah yang diukur adalah periode fertil dan fertilitas sperma. Data yang diperoleh dianalisis dengan analisis sidik ragam, dan untuk mengetahui perbedaan antar perlakuan diuji dengan menggunakan uji jarak berganda Duncan. Hasil penelitian menunjukan bahwa lama penyimpanan semen …

Evaluation Of Mcf-7 Breast Cancer Cell Cytotoxic And Antioxidant Activities Of Peptide Fractions From Symbiotic Bacteria Of Jellyfish Catostylus Sp., Eka Sry Wahyuni, Ahyar Ahmad, Muhammad Nasrum Massi, Sofa Fajriah, Randi Rimpung, Muh. Akbar Ardiputra, Harningsih Karim, Irda Handayani

Karbala International Journal of Modern Science

Marine-derived symbiotic microorganisms are recognized as a promising source of bioactive compounds with potential therapeutic applications, yet research on jellyfish-associated bacteria remains limited. This study examines the bioactivity of peptide fractions derived from symbiotic bacteria isolated from the jellyfish Catostylus sp., collected from the coastal waters of South Sulawesi, Indonesia, with a focus on their anticancer and antioxidant properties. Following sample collection, the symbiont bacteria were isolated, enzymatically hydrolyzed, and purified before their biological activity was evaluated. Preliminary cytotoxicity screening using the brine shrimp lethality assay revealed that the extracellular peptide fraction (5–10 kDa) and intracellular peptide fraction (3–5 kDa) …

Global Deep-Sea Hydrothermal Deposit Metagenomes And Metagenome-Assembled Genomes Over Time And Space, Emily St John, Anna-Louise Reysenbach

Center for Life in Extreme Environments Publications

Actively venting high temperature deep-sea hydrothermal vent deposits along tectonic spreading centers and in backarc basins harbor a rich diversity of thermophilic Bacteria and Archaea, many of which have no representatives in cultivation nor any genomic representation in databases. Here, in order to produce a global-scale time series metagenomic resource for studying the microbial functional and genomic diversity in these high temperature ecosystems, we obtained 70 metagenomes from collections across spatial and temporal gradients from 21 different vent fields spanning 16 years (1993-2009). The dataset (Deep-Sea Hydrothermal Vent dataset (DSV70)) includes 3.56 Tbp of raw DNA sequence reads, that have …

Role Of The Etv5/P38 Signaling Axis In Aggressive Thyroid Cancer Cells, Jerry H Houl, Rozita Bagheri-Yarmand, Muthusamy Kunnimalaiyaan, Paola Miranda Mendez, Joseph L Kidd, Ali Dadbin, Andrea Ruiz-Jurado, Parag A Parekh, Ying C Henderson, Nikhil S Chari, Aatish Thennavan, Reid T Powell, Clifford C Stephan, Xiao Zhao, Anastasios Maniakas, Roza Nurieva, Naifa L Busaidy, Maria E Cabanillas, Ramona Dadu, Mark Zafereo, Jennifer R Wang, Stephen Y Lai, Marie-Claude Hofmann

Faculty, Staff and Student Publications

Patients with poorly differentiated thyroid cancer (PDTC) and anaplastic thyroid cancer (ATC) face a much poorer prognosis than those with differentiated thyroid cancers. Around 25% of PDTCs and 35% of ATCs carry the BRAFV600E mutation, which constitutively activates the MAPK pathway, a key driver of cell growth. Although combining BRAF and MEK inhibitors can shrink tumors, resistance often develops. The exact cause of this resistance remains unclear. We previously found that in PDTC and ATC cells, the BRAFV600E mutation is strongly linked to the expression of ETV5, a transcription factor downstream of the MAPK pathway. In the current study, we …

Grow Your Own Microgreens, Stephen Beck

All Current Publications

This fact sheet reviews the steps and materials needed to grow microgreens, such as radish, broccoli, sunflower, or pea shoots. Microgreens are fast and easy to grow and a healthy addition to your diet.

Why Agtech Startups Fail? Evidence From Global Shutdown Patterns In 2025 And The Cost-Adoption Mismatch Effect, Ankit Chandra, Ishani Lal

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Agricultural technologies are widely seen as a driver for sustainability, resilience, and food-system transformation. Yet in 2025, agtech startup across North America, Europe, Asia, and Africa experienced multiple shutdowns. Although each company closed for its own reasons, the failures highlight consistent underlying patterns. We analyze 18 publicly reported shutdowns across controlled-environment agriculture (CEA), robotics, insect protein, digital platforms, sensors, and ag-biotechnology. We find that most ventures struggled not with scientific feasibility but with economic and adoption dynamics at the farm level. We identify a Cost-Adoption Mismatch Effect, in which the capital and operational burden of a technology exceeds farmers’ capacity …

A233: Digital Empowerment And Governance Innovation For Smart Sports Community Development In Urban China, Li Liu, Xuekai Qi, Jie Wu, Wei Shen

International Journal of Physical Activity and Health

Smart sports communities are emerging as a crucial component of China’s National Fitness Strategy, driven by digital empowerment and governance innovation. This study examines the historical evolution of urban community sports and investigates how technological innovations enhance governance, management, service efficiency, and user experience in the development of smart sports communities. Method: This research employs an empirical case study in the Yangtze River Delta region, encompassing 25 grassroots communities across cities such as Nanjing, Jiaxing, Shanghai, Hefei, and Wuhu. A historical analysis of policy documents, academic research, and media reports provides longitudinal insights into the transformation of urban community sports …

A270: Exerciser Adoption Of Personalized Workout Plan In Ai-Powered Fitness Apps: The Technology Affordance Perspective, Yike Jiao, Zhao Du, Ziyan Deng

International Journal of Physical Activity and Health

With the proliferation of artificial intelligence in the sports industry, AI-powered fitness apps that offer personalized fitness services are becoming increasingly prevalent. The personalized workout plan, a popular feature of AI-powered fitness apps, is being added to a growing number of fitness apps and embraced by a rapidly expanding user base. Personalized workout plans are tailored to exercisers’ fitness goals, demographics, body features, preferences, and skill levels. Although this feature is expected to enhance exerciser engagement and improve their satisfaction, scholarly works investigating the factors that drive and hinder exercisers from adopting it remain scarce. Based on the technology affordance …

A276: A Grounded Theory Study: Could Outdoor Hiking Foster Meaningfulness In Life For Urban Gen-Z?, Weikang Liao, Li Li

International Journal of Physical Activity and Health

Urban Generation Z (youth born between 1995 and 2009 in cities) has grown up in highly digitized and urbanized environments, facing contemporary challenges such as social alienation and a lack of meaning in life. While outdoor hiking is recognized as a stress-relief activity, its specific mechanisms for fostering meaning in life among Generation Z remain unclear. Existing studies predominantly focus on the physical and mental health benefits of outdoor activities, neglecting the dynamic construction of meaning in life and particularly overlooking the unique characteristics of this generational cohort. This study aims to uncover the distinct pathways through which urban Generation …

A305: The International Communication Path Of Table Tennis Sports Presentation, Mingchen Xu, Xiyu Zhao

International Journal of Physical Activity and Health

Table tennis carries a unique mission of cross-cultural communication. In 1971, the "Ping-Pong Diplomacy" with the strategic wisdom of "a small ball turning a big ball" set a classic model for sports public diplomacy. As a carrier of national cultural symbols, the international communication of table tennis should rely on major events to tell the table tennis story to achieve presentation and Presentation. As a key for event communication, in table tennis events, is driven by the synergy of three-dimensional elements: "competitive core + technological extension + commercial drive". Specifically, through the organic combination of visual and auditory elements and …

A041: “Sports Yuan”: The Body Expression Of Young Women's Digital Participation In Sports, Zongrui Ye

International Journal of Physical Activity and Health

With the rise of social media and the popularity of outdoor sports, more young women are sharing sports-related photos and videos online, giving rise to the “sports goddess” phenomenon. This trend has sparked debate, being seen as a reflection of modern women’s active and healthy lifestyle, yet criticized for excessive image focus, deviating from true sports spirit. This research aims to analyze the bodily expression behind the phenomenon and its impact on sports culture, using self-presentation theory and power-disciplinary theory to explore how social media shapes women’s self-expression. This study uses theoretical analysis based on Erving Goffman’s self-presentation theory and …

A051: Spatial Distribution Pattern And Influencing Factors Of Ice And Snow Tourism Resources In Shandong Province, Yu Zhang, Xinlong Zhao, Yan Wang

International Journal of Physical Activity and Health

At present, most studies focus on the three provinces in Northeast China, the Beijing-Tianjin-Hebei region, and Inner Mongolia, where the basic conditions of ice and snow tourism are relatively superior, while the spatial distribution characteristics of ice and snow tourism in Shandong Province are relatively scarce. In view of this, this study adopts ArcGIS spatial analysis technology to deeply explore the spatial distribution characteristics of ice and snow tourism resources in Shandong Province, and comprehensively analyze its influencing factors, aiming to provide theoretical suggestions and evidence-based policy references for optimizing the spatial layout of ice and snow tourism resources in …

A107: Multidimensional Influencing Factors And Psychosocial Mechanisms Of Eating Disorders In Female Athletes, Yajing Zhao, Peizhen Zhang, Lumeng Yang

International Journal of Physical Activity and Health

Eating disorders (ED) are prevalent mental health conditions that significantly threaten the physical and psychological well-being of female athletes. The risk of ED is driven by the interplay of multiple factors, including sport-specific training environments, psychological vulnerabilities, and sociocultural pressures. Although existing research has identified several independent risk factors, a systematic understanding of the sport-related psychosocial mechanisms remains insufficient. This study aims to synthesize empirical evidence from the past five years to elucidate the multidimensional pathways of ED in female athletes, with a particular focus on intervention targets from a sport psychology perspective. A systematic literature review was conducted, retrieving …

A133: Self-Aggrandizing In Badminton Teaching Short Videos Affects Learning Willingness, Mingxiang Yi, Yuanjing Huang

International Journal of Physical Activity and Health

Based on the new era of digital sports development, self-media platforms have become an important carrier of sports teaching communication; however, few studies have investigated the relationship between self-aggrandizing behavior during sports teaching on self-media platforms and platform users' learning intention. In this study, we take Jittery self-media platform as an example to explore the chain path that creators' boasting behavior (H1) positively affects users' learning intention (H2) by enhancing users' trust (H3), and to verify the mediating effect of trust in the relationship between boasting behavior and learning intention, as well as the two-way influence between badminton short video …

A135: 6-Week Intermittent Fasting And Aerobic Exercise: Effects On Body, Cardiovascular, And Sleep In Masked Obese Men, Boran Zhang, Meng Li, Runsheng Nie, Yuting Fu

International Journal of Physical Activity and Health

Masked obesity (MO), defined by normal BMI with elevated body fat percentage, poses elevated health risks. This randomized trial compares combined intermittent fasting (IF) and aerobic exercise (AE) with single-modality interventions in young males, assessing body composition, cardiovascular function, and sleep quality to establish optimal evidence-based interventions. A total of 36 participants were randomized into three groups (n=12 each): (1) intermittent fasting combined with aerobic exercise (IF+AE), (2) IF-only, and (3) AE-only. Baseline assessments included body composition (weight, fat/lean mass, waist-to-hip ratio), cardiovascular markers (carotid intima-media thickness [CIMT], ejection fraction), and Pittsburgh Sleep Quality Index (PSQI). The IF+AE group alternated …

A183: Research On The Path Of The Digital Development Of Sports Culture, Hanxiao Wu

International Journal of Physical Activity and Health

The party's 20th report proposed to build a sports power and promote cultural confidence and self-improvement. Campus sports culture needs to borrow digital technology innovation breakthroughs: through intelligent technology to promote the Chinese sports spirit into virtual events, wisdom teaching scenarios, combined with AI and big data implementation physical training and two-way conduction of core values, build "digital technology can assign movement practice-cultural gene deepening spiritual identity" synergy mechanism, for sports power and cultural confidence provide operational innovation path. To study the path of digital development of sports culture on the comprehensive development of teenagers by means of literature, expert …

A211: Ai-Driven Strategic Frameworks For Sport Event Propagation Under Media Affordance Theory, Wenqian Yao, Jingwang Zhang

International Journal of Physical Activity and Health

The advancement of intelligent technology has transformed interaction models across communication ecosystems, encompassing production, dissemination, and consumption. Exemplified by the Paris Olympics’ 180+ intelligent communication domains, “AI + Sports” has emerged as an industry paradigm. This study employs media affordance theory to analyze AI’s transformative role in sports communication and associated ethical challenges, offering insights for cultivating a robust digital sports ecosystem. Method: This study investigates the application pathways of artificial intelligence in 20 major sports events held between 2000 and 2024. Data on application frequency, audience feedback, and other relevant metrics w collected to analyze the impact patterns of …

A228: Preservation And Dissemination Of Taijiquan Culture In Digital Perspective, Songqi Wang, Bin Yan

International Journal of Physical Activity and Health

One of China’s most iconic cultural traditions has increasingly gained: Taijiquned global recognition: Taijiquned. However, rapid digitalization has significantly changed young people's cultural preferences and participation habits, posing challenges to traditional methods of cultural preservation, which often fail to resonate with youth, creating a noticeable disconnect. This study examines the potential of digital communication—especially short videos, livestreaming, and social media—to preserve and disseminate Taijiquan culture through interactive, youth-oriented methods. Specifically, it empirically explores whether digital media strategies effectively improve younger generations’ cultural awareness, participation motivation, and cultural identity, aiming to provide practical insights for digital heritage protection. A randomized controlled …

Diet-Responsive Genetic Determinants Of Intestinal Colonization In The Yeast Candida Albicans, Musfirat Shubaita, Mazen Oneissi, Elena Lindemann-Pérez, Cecilia Fadhel Alvarez, Anne-Marie Krachler, Diana M Proctor, J Christian Pérez

Faculty, Staff and Student Publications

Dietary components influence microbial composition in the digestive tract. Although often viewed as energy sources, dietary components are likely to shape microbial determinants of intestinal colonization beyond metabolism. Here, we report that a dietary long-chain fatty acid enhances the yeast Candida albicans colonization of the murine gut partly by eliciting modifications to the fungal cell surface. Mice fed an oleic acid-rich diet were readily colonized by C. albicans and exhibited higher fungal load in feces compared with rodents fed an isocaloric control diet. Surprisingly, β-oxidation, a catabolic process to break down fatty acids for energy production, was dispensable for C. …

The Food Ethics, Sustainability And Alternatives Course: A Mixed Assessment Of University Students’ Readiness For Change, Charles Feldman, Stephanie A. Navarro Silvera

Department of Nutrition and Food Studies Scholarship and Creative Works

Growing interest in food sustainability education aims to increase awareness of food distribution systems, environmental degradation, and the connectivity of sustainable and ethical food practices. However, recent scholarship has questioned whether such pedagogical efforts are meaningfully internalized by students or lead to sustained behavioral change. Prior studies document persistent gaps in students’ understanding of sustainability impacts and the limited effectiveness of existing instructional approaches in promoting transformative engagement. To address these concerns, the Food Ethics, Sustainability and Alternatives (FESA) course was implemented with 21 undergraduate and graduate students at Montclair State University (Montclair, NJ, USA). Course outcomes were evaluated using …

Direct Effects Of Capsaicin On Voltage-Dependent Calcium Channels Of Mammalian Skeletal Muscle, Dmytro Isaev, Tatiana Prytkova, Badarunnisa Mohamed, Mohamed Omar Mahgoub, Keun Hang Susan Yang, Murat Oz

All Works

Capsaicin, a naturally occurring polyphenol, is known to affect energy expenditure and muscle fatigue and modulate contractions in skeletal muscle. The L-type Ca²⁺ channels are known to be an important ion channel involved in the various muscle functions and the effect of capsaicin on the skeletal L-type Ca²⁺ channels is currently unknown. In this study, the effects of capsaicin and capsaicin analogs on depolarization-induced Ca²⁺ effluxes through L-type Ca²⁺ channels in transverse tubule membranes from rabbit skeletal muscle and L-type Ca²⁺ currents recorded using the whole-cell patch clamp technique in rat myotubes were examined. Capsaicin, …

Investigating Host Factors In Coronavirus Assembly And Egress, Surovi Binte Sharmin Mohona

Dissertations

Coronavirus (CoV) structural proteins congregate at the ER-Golgi intermediate compartments (ERGIC) prior to virus budding into the organelle lumen. Subsequent trafficking and fusion of virus-containing, ERGIC-derived vesicles then release nascent viruses from infected cells. Our findings in this dissertation illuminate mechanisms by which CoV structural proteins coalesce at the virus assembly and budding sites. The cytoplasmic tails of all CoV spike (S) glycoproteins contain variants of a consensus KxKxx coatomer-binding motif. Host coatomer complexes facilitate retrograde trafficking of client cargo proteins via recognition of such motifs. Utilizing reverse genetics methodologies, we introduced variations in the S coatomer motif in a …

Decoupling Of Reproduction And Lifespan Trade-Off In Caenorhabditis Elegans Through Microbial Programming, Nora M. Villafuerte

LSU Master's Theses

The microbiome is increasingly recognized as a major regulator of host physiology. Using the Caenorhabditis elegans model, we investigated how natural commensal bacteria modulate host reproduction and lifespan through internal egg hatching, a phenotype in which retained embryos hatch within the maternal body. Screening strains from the CeMbio collection identified four focal strains Ochrobactrum BH3, Lelliottia JUb66, Pantoea BIGb0393, and Enterobacter CEent1 that significantly increased internal egg hatching during the late reproductive period. This contrasts with pathogenic bacteria, which induce early-onset internal egg hatching. Across the focal strains, worms exhibited reduced total brood size but an expanded reproductive window relative …

Assessing Localized Recovery And Synchrony Of An Endangered Wetland Species., Aiyana Reissman

Theses

The state and federally endangered light-footed Ridgway’s rail (LFRR; Rallus obsoletus levipes) is central to regional conservation, yet the drivers of its population fluctuations remain poorly understood. The fragmented nature of coastal wetlands in Southern California is threatening the existence of endemic species like the LFRR. Tidal inundation, severe storms, and overpredation threaten the survival of LFRR. This study addresses population fluctuations by analyzing the spatial and temporal drivers of LFRR populations to inform effective, site-specific management.

To do this, a 45-year (1980–2024) LFRR census data set across 16 wetlands throughout its range in California was synthesized. A multi-faceted …

Tumor-Infiltrating Bacteria Disrupt Cancer Epithelial Cell Interactions And Induce Cell-Cycle Arrest, Jorge Luis Galeano Niño, Falk Ponath, Victor A Ajisafe, Clara R Becker, Andrew G Kempchinsky, Martha A Zepeda-Rivera, Javier A Gomez, Hanrui Wu, Jessica G Terrazas, Heather Bouzek, Elizabeth Cromwell, Pritha Chanana, Matthew Wong, Ashish Damania, Michael G White, Y Nancy You, Scott Kopetz, Nadim J Ajami, Jennifer A Wargo, Christopher D Johnston, Susan Bullman

Faculty, Staff and Student Publications

Tumor-infiltrating bacteria are increasingly recognized as modulators of cancer progression and therapy resistance. We describe a mechanism by which extracellular intratumoral bacteria, including Fusobacterium, modulate cancer epithelial cell behavior. Spatial imaging and single-cell spatial transcriptomics show that these bacteria predominantly localize extracellularly within tumor microniches of colorectal and oral cancers, characterized by reduced cell density, transcriptional activity, and proliferation. In vitro, Fusobacterium nucleatum disrupts epithelial contacts, inducing G0-G1 arrest and transcriptional quiescence. This state confers 5-fluorouracil resistance and remodels the tumor microenvironment. Findings were validated by live-cell imaging, spatial profiling, mouse models, and a 52-patient colorectal cancer cohort. Transcriptomics reveals …