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Degradation And Metabolic Analysis Of Synthesized Quorum-Sensing Signals By The Model Eukaryote Chlamydomonas Reinhardtii, Adam Josef Bach

Theses and Dissertations

Bacterial organisms can “talk” with one another on a colony-wide scale using a communication system known as quorum-sensing (QS) via chemical signals. These signals are produced at a basal rate throughout the life of the bacteria and are classified as autoinducers, which diffuse across the cell membrane. Once the critical concentration is reached, they begin to diffuse back into the cell and initiate a phenotypic change. As these signals exist outside the membrane of their producer, they are also accessible to other organisms. It has already been noted that some plant species uptake these compounds through their roots and break …

2nd Place Research Paper: Shaping The Ape: Storytelling, Cultural Reflection, And Boundary Negotiation In Human-Ape Zoo Encounters, Abigail Bond Halling

Kevin and Tam Ross Undergraduate Research Prize

The typical Western zoo functions as a unique environment in which animals are frequently positioned as subjects of education and entertainment through the medium of storytelling. In this sense, the zoological institution can also be read as an extension of popular Western constructions surrounding nonhuman animals, particularly those who are symbolically charged characters in our collective imaginary– the great apes. Given the morphological, behavioral, and psychological similarities between humans and other great apes (chimpanzees, gorillas, and orangutans), nonhuman great ape enclosures at the Western zoo become spaces where humans are most radically confronted with navigating the boundaries of the “human-animal …

Volume 17, Christian O’Neill, Kyara Greene, Savva Sidorov, Laura Bisaillon, Luke Clemmer, Hannah Gordon, Kitt Benson, Taylor Blount, Rachel Danzitz, Nicholas Duellman, Chase Gionis, Hima Fernando, Seth Franzyshen, Onyx Gonzalez, Bryan Lin, Samantha Start, Ysabel Wells, Maggie Duncan

Incite: The Journal of Undergraduate Scholarship

Introduction Dr. Amorette Barber, Director, Office of Student Research

From the Editor Dr. Hannah Dudley-Shotwell

Cover Artist’s Statement Maggie Duncan

On Mentoring Dr. Yulia Uryadova

Ukrainian Resistance in the Face of Russification: Nestor Makhno and Anarchism

by Christian O’Neill

Life Vest by Kyara Greene

Isolation and 16S rRNA Identification of Bacteria from Fire Department Connection Pipe by Savva Sidorov

The Effectiveness of Planned Exercise in Reducing ADHD Symptoms in Children by Laura Bisaillon & Luke Clemmer

Linguistic Analysis on Confidence and Communication Strategies with Disparities Between Sign Fluency and Hearing Impairment by Hannah Gordon

Freedmen in Indian Territory by Kitt …

Effects Of M6a Dna Methylation By Bacterial Methyltransferase In Colorectal Cancer, Fabian Alejandro Mendoza Galvan

Dissertations and Theses (Open Access)

Effects of m6A DNA methylation by bacterial methyltransferase in colorectal cancer Fabian Alejandro Mendoza Galvan Advisory Professor: Angela H. Ting, Ph.D. Fusobacterium nucleatum animalis (Fna) is found in the human oral cavity and gut. A distinct clade of Fna is primarily enriched in the tumor microenvironment (TME) and within colorectal cancer (CRC) cells. This clade DNA methylation pattern is primarily catalyzed by a cell-cycle regulated methyltransferase (CcrM) ortholog, M.FnI, that targets the GANTC sequence motif through methyl-6-Adenine (m6A) DNA methylation. We hypothesized that M.FnI enzyme can induce m6A methylation abnormalities in CRC cells to promote cancer progression. Evidence for endogenous …

A Tractable Nematode Model For The Emerging Fungal Pathogen, Candida Auris, Melissa Martinez

Dissertations and Theses (Open Access)

Candida auris is an emerging multidrug-resistant opportunistic fungal pathogen. It

poses a significant threat to immunocompromised patients and those with prolonged stays in

healthcare facilities. C. auris spreads rapidly in health care facilities through enhanced

colonization of skin and abiotic surfaces. The genetic factors contributing to this ability and to

the virulence, drug resistance, and stress-tolerant nature of C. auris are largely unknown.

Current animal models of C. auris infections are not well standardized and report conflicting

results. Additional animal models of virulence are needed, especially those amenable to high-

throughput analysis. The nematode Caenorhabditis elegans has been validated as …

Characterization Of Staphylococcus Aureus Clinical Isolates From Deep Musculoskeletal Infections For An In Vitro Biofilm Model For Phage Therapy, Raquel C. Luna

Dissertations and Theses (Open Access)

The rise of multidrug-resistant (MDR) infections is a growing concern in the treatment of deep musculoskeletal infections (MSKIs) and orthopaedic device-related infections (ODRIs). Staphylococcus aureus is responsible for about one third of these infections due to its strong biofilm forming capacity, presence on human skin, and the large number of antibiotic-resistant variants. Bacteriophage (phage) therapy is now being considered as a treatment for MDR MSKIs/ODRIs. We are investigating the use of phage encapsulated into or adsorbed onto biodegradable microspheres for local delivery to the site of these MDR infections. We determined that the anti-staphylococcal phage K effectively lyses the S. …

Membrane Organization Of Rheb And Rhoa: Roles In Function And Allosteric Druggability, Chase M. Hutchins

Dissertations and Theses (Open Access)

Small GTPases of the Ras superfamily are membrane-bound molecular switches that regulate nearly all major cellular processes, and their dysregulation drives cancers, neurological disorders, and metabolic diseases. While anchored to cellular membranes, the catalytic domains of small GTPases undergo orientation dynamics, adopting distinct configurations that can occlude or expose effector-binding surfaces and modulate signaling output. These dynamics have been characterized in the Ras oncoproteins, but whether they extend across the superfamily and influence allosteric druggability has remained unknown.

In this dissertation, I address these questions through Rheb and RhoA, two small GTPases with complementary differences in subfamily lineage, membrane localization, …

Developing An Independent Shallow-Water Survey For The Western Rock Lobster Fishery: Tracking Pre-Recruitment Abundance And Habitat Change, Lucy Rudd, Simon De Lestang, Owen Young, Benjamin Hebiton

Fisheries Research Reports

Background

Puerulus settlement has proven to be a good forecaster of legal recruitment of Western Rock Lobster entering into the commercial and recreational fisheries. However, in 2011, this relationship broke down, with average puerulus settlement levels not being reflected in commercial catch rates four years later. Some northern areas showed unexpectedly low catch rates, while other southern areas showed unexpectedly high catch rates. Since this change followed the 2011 marine heatwave, it was assumed that this event changed some component(s) which were important to the growth and survival of juvenile lobsters. It was determined that a better understanding of this …

A Novel Method Of Protein Purification Using Hedgehog Proteins As An Inducible, Self-Cleaving Tag, Antonia Kladias

Undergraduate Honors Theses

Because removing extraneous purification tag sequences from native proteins can be challenging and costly, there is demand for simple all-in-one techniques that directly furnish a tagless, functional protein-of-interest (POI). Current tagless purification systems are based on intein biochemistry; however, intein-based purification requires expensive, specialized resins and the POI often must be fused to the intein’s C-terminus. In this thesis, I present a novel approach for tagless POI purification in which the Drosophila melanogaster hedgehog protein catalytic domain (HhC) is repurposed as a small-molecule inducible, self-cleaving element dubbed Gtag. With Gtag, the POI is fused to the N-terminus of HhC/Gtag with …

Enrichment Of The Thermophilic Archaea Gearchaeales And Calditenuis In Lab Culture, Walter Saldivar

Electronic Theses, Projects, and Dissertations

Numerous microbes on Earth have yet to be grown in pure cultures and studied in the laboratory. Cultivation-independent genomic methods, such as metagenomics, can help us predict their metabolic capabilities, but cultivation remains valuable for testing these predictions. These uncultivated microbes are very diverse, especially within hot springs. Examples of these uncultivated microbes include the genus Calditenuis (formerly Aigarchaeota Group 1) and the order Gearchaeales; they are often abundant in terrestrial hot springs such as Great Boiling Springs (GBS) in Nevada. Previous work allowed for the establishment of laboratory enrichment cultures inoculated from sediments of GBS containing Calditenuis and …

Understanding The Role Of Toxs As A Bile Sensing Component Of The Toxrs Virulence Regulatory System In Vibrio Spp., Minje Kim

Dartmouth College Ph.D Dissertations

Pathogenic Vibrio bacteria, such as V. cholerae and V. parahaemolyticus, colonize in the human small intestine to cause severe gastrointestinal disease. During the infection, these bacteria encounter bile salts, antimicrobial cholesterol metabolites secreted into the intestine. Pathogenic Vibrio species have evolved to utilize bile salts as signals to regulate virulence. The signaling depends in part on ToxRS, a conserved co-component transmembrane transcription regulator. ToxRS consists of the transcription factor ToxR and its membrane-tethered binding partner ToxS. ToxS is required for ToxR stability and full transcriptional activity. Although bile salts are known to influence ToxRS-dependent virulence gene expression, the molecular …

Clawing Our Way To Inclusion: Identifying Minority Students’ Barriers To Entering The Veterinary Medicine Profession, Danielle Reeves

Animal Science Undergraduate Honors Theses

Veterinary services are being utilized more each year as pet owners are increasingly viewing and treating their pets like family members. Due to this changing perspective, the veterinary profession has grown significantly. Yet, despite this change, there is a lack of ethnic/racial diversity within the veterinary field. The purpose of this study was to determine the factors and/or barriers associated with minority students' desire or lack of desire to pursue a career in veterinary medicine. This study used an online survey tool to collect qualitative and quantitative data from 423 undergraduate students at the University of Arkansas during the spring …

Analyzing The Effects Of Message Testing On The Public's Opinion About Gene Editing In Agriculture And Medicine, Luke Sanford Wimpy

Graduate Theses and Dissertations

This paper will present results from the final survey of a multi-stage project examining factors that influence opinions about gene editing in agriculture and medicine. In this final survey, 3,200 U.S. respondents were recruited and randomized to view one of eight videos that communicated the safety of gene editing to determine which factors are most influential in changing opinions. The eight videos were designed based on data collected from focus groups and three previous surveys in this multi-stage project. The eight videos varied by three attributes: messenger, message, and application. The messenger in the videos was either a researcher or …

Understanding Vaccine Hesitancy In Veterinary Medicine: A Review Of Client Attitudes, Grace Moore

Undergraduate Honors Capstone Projects

Uncertainty toward vaccination, long recognized as a public health concern in human medicine, is increasingly recognized within veterinary contexts, posing risks to both animal and community health. This literature review examines the development, causes, and implications of reduced vaccine confidence in veterinary medicine, with emphasis on small animal and some large animal practices. The review combines historical and current research to explore how vaccination perceptions have evolved among veterinarians and clients, and how factors such as the COVID-19 pandemic have shaped current attitudes.

The literature highlights recurring themes surrounding vaccine reluctance in veterinary settings. Client perceptions of safety, efficacy, and …

Behind The Smile: A Qualitative Study On Dentist Burnout And Workplace Satisfaction In Cache Valley, Luke Thomas Hatch

Undergraduate Honors Capstone Projects

This qualitative research study examined satisfaction, stressors, and burnout among dental professionals in Cache Valley, Utah. Eight practitioners at various points in their careers were interviewed with a range of specialties including general dentistry (n = 3), pedodontics (n = 3), oral and facial surgery (n = 1), and orthodontics (n = 1). Analysis of the interviews showed that level of clinical autonomy was a primary factor influencing burnout levels. Stress levels in more experienced practitioners and owners tended to be lower than those who worked in multiple practices (multi-doctor) or with corporations. Individuals in these environments reported higher levels …

Application Of Path Analysis To Determine The Impact Of Age And Sex On The Neural Circuits That Promote Nicotine Withdrawal Severity, Veronika Espinoza

Open Access Theses & Dissertations

The rising prevalence of electronic cigarette (e-cigarette) use presents a major public health concern, particularly in adolescent females who are most susceptible to nicotine use and its long-term negative health consequences. The goal of this dissertation was to utilize preclinical rodent models to understand how group differences in age and sex influence neural activation patterns in brain circuits believed to promote nicotine withdrawal. Prior pre-clinical work, conducted largely in male rodents, has established that there are two major brain pathways that modulate the behavioral effects of nicotine withdrawal. These include the mesolimbic and medial habenula-interpeduncular nucleus (MHb-IPN) pathways. To assess …

Determining Zap-70 Signaling Pathways Mediated By Ccr7 In Tcr T Cell Activation, Vivian Torres

Open Access Theses & Dissertations

Activation of T lymphocytes is the main event that occurs in the adaptive immune response after engagement of the T cell receptor (TCR) with the peptide of the major histocompatibility complex (pMHC) molecules that are demonstrated by antigen presenting cells [1]. A key step in this process is the recruitment and activation of the zeta chain associated protein of 70 kDa (ZAP-70). ZAP-70 couples TCR engagement to downstream intracellular signaling pathways through phosphorylation of tyrosine residues within intracellular tyrosine activation motifs (ITAMs) [2]. Although there have been extensive studies on the signaling response through TCR activation, the regulatory influence of …

Molecular Mechanisms Of Pfas Induced Protein Dysfunction: Implications For Neurodegenerative Risk, Daisy Lee Wilson

Open Access Theses & Dissertations

Per- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants with widespread human exposure and well-established links to adverse health outcomes. However, despite increasing epidemiological and experimental evidence, the molecular mechanisms underlying PFAS-induced neurotoxicity remain incompletely defined. This dissertation investigates PFAS toxicity across biological scales, integrating protein biophysics with neuronal cell models to elucidate pathways relevant to neurodegeneration. At the molecular level, we demonstrate that PFAS disrupts the structure and function of globular proteins, including the impairment of α-lactalbumin's calcium-binding capacity and of β-lactoglobulin's retinol binding, through concentration-dependent conformational destabilization. By applying these findings to a neuronal model, we show that …

Forage Responses To Biological Inoculant And Seed Rates In The Intermountain West, Roy Reid

All Graduate Theses and Dissertations, Fall 2023 to Present

Improving the efficiency of fertilizer and seed use is increasingly important for forage producers in the Intermountain West, where alfalfa and silage corn form the foundation of dairy and livestock feed. This thesis evaluates two strategies designed to boost crop productivity: microbial fertilizer coatings and variable-rate seeding.

The first study examined whether Powercoat®, a fertilizer-applied microbial inoculant, could enhance yield, forage quality, or soil phosphorus availability in alfalfa grown on four commercial fields in northern Utah. Across all locations, fertilizer increased yield at only two sites, and Powercoat did not provide additional improvements in yield, forage quality, or soil nutrient …

Alfalfa Yield, Quality, And Nutrient Use Response To Pivot Sprinkler Technology, Deficit Irrigation, And Genetics, Dakota Boren

All Graduate Theses and Dissertations, Fall 2023 to Present

In water limited environments, alfalfa is often criticized for its high water use, prompting implementation of new irrigation technologies, deficit irrigation, and adapted crop genetics. Cumulative potential benefits from combining, or stacking, these water-optimizing practices have not been identified. The objective of this on-farm research study was to determine how irrigation technologies, deficit irrigation, genetics, and combinations of these practices impact alfalfa yield and forage quality. Trials were conducted at three sites in Utah from 2020 to 2022 on young alfalfa stands (establishment and first one to three production years). Treatments included five irrigation technologies, four irrigation rates, and two …

Understanding Impact Of Redox Potential On Microbial Community Of Cheddar Cheese During Aging, Abrianna Y. Paez

All Graduate Theses and Dissertations, Fall 2023 to Present

Cheese ripening is a complex process that shapes the flavor, texture, and overall quality of cheese, yet many of the factors that control how these changes occur are still not fully understood. One factor that may play an important role is redox potential, which describes the balance between oxidation and reduction reactions happening inside the cheese. These reactions can influence both the activity of bacteria and the chemical processes that create flavor. Past efforts to control redox using chemical additives often harmed beneficial bacteria, limiting their usefulness.

In this study, we tested the use of mechanical disruption and gas exposure …

Food-Form Delivery Of Spore-Forming Probiotics As An Alternative To Gavage In Animal Models, Aubreyona Faith Migliori Skalka

All Graduate Theses and Dissertations, Fall 2023 to Present

Western dietary patterns are characterized by nutrient-poor, calorie-rich diets and have been linked to poor health outcomes and gut inflammation phenotypes. Probiotics are often taken to support gut health, but many beneficial bacteria do not survive transport through the digestive system to produce any effect. Spore-forming bacteria, such as Bacillus coagulans, are more resilient due to their hardy outer coat which aids in survival through many obstacles encountered in the gastrointestinal tract, including acidic stomach conditions. In research studies, probiotics are usually given to mice through a procedure called oral gavage, where a tube is used to deliver the …

Effects Of Serum And Commercial Serum-Free Media On Development Of In Vitro Produced (Ivp) Embryos In Cattle, Kyla Miller

All Graduate Theses and Dissertations, Fall 2023 to Present

Serum-containing media has been shown to increase incidence of large offspring syndrome and to alter gene expression, protein synthesis, and cell signaling. These factors together can result in increased pregnancy loss when combined with assisted reproduction technologies. However, there are very few direct comparisons of commercial serum-free media (IVF Bioscience™) and serum-containing media on both IVF and cloned embryo development in cattle. The aim of this study was to determine the role of serum in oocyte maturation and subsequent embryo development. Three culture systems, including in vitro maturation (IVM) and in vitro culture (IVC), were used in this study. Both …

Validation Of Rice Tissue Sampling For In-Season Nutrient Management Of Nitrogen, Phosphorus, And Potassium, Tanner Smith

Graduate Theses and Dissertations

Nitrogen (N), phosphorus (P), and potassium (K) are the 3 most important nutrients involved in rice (Oryza sativa L.) growth and development. Tissue sampling has been proven to be a potential tool to provide inseason information about plant nutrient concentrations at specified timings and growth stages. With increased interest in tissue sampling and improving nutrient management effectiveness, more information is needed to validate plant tissue concentrations at which additional fertilizer inputs may be warranted. Experiments were conducted in 2024 and 2025 at the Rice Research and Extension Center (RREC) near Stuttgart, Ark.; Pine Tree Research Station (PTRS) near Colt, Ark.; …

Lumbopelvic Kinematics And Muscular Co-Contractions In Walking Prior To And Immediately Following A Bout Of Prolonged Standing., Stephanie Cotariu

Graduate Theses and Dissertations

Background: Prolonged standing is a common daily activity in a wide array of occupations, and approximately 44% of sample populations develop low back pain (LBP) during prolonged standing. Prolonged standing-induced LBP developers (“Pain Developers”) are three times more likely to develop clinical LBP and can therefore be considered a pre-clinical LBP population. Changes in typical gait have been observed in LBP patients, and walking has been hypothesized as being protective or preventive against LBP development. However, it is unknown whether Pain Developers exhibit similar kinematic adaptations to chronic LBP patients during common daily activities, such as walking. The exact muscular …

Optimized Ultrasound Imaging Of Phase-Change Nanodroplets, Charles R Dyall, Dmitry Nevozhay, Andy Liu, Trevor M Mitcham, George J Lu, Konstantin V Sokolov, Richard R Bouchard

Faculty, Staff and Student Publications

Phase-change nanodroplets (PCNDs) continue to generate significant research interest due to their potential to extravasate into tissue, to be targeted for molecular imaging and drug delivery, and to undergo an induced phase-change to "activated" microbubbles (MBs) for ultrasound (US) imaging. To accurately quantify molecular markers, however, one assumes a consistent proportion of PCNDs in a region of interest (ROI) are stably activated and imaged. Herein we present a framework for developing a diagnostic sequence that is optimized for PCND activation uniformity, contrast, and acquisition time. To develop this framework, activation was examined at three scales of increasing complexity: single, adjacent, …

Potential Impact Of Parasites In The Transmission Of Chronic Wasting Disease, Paulina Soto, Rodrigo Morales

Faculty, Staff and Student Publications

No abstract provided.

Exploring The Biocultural Profile Of The Undocumented Populations Of The Sonoran Corridor: A Skeletal Assessment Of The Prevention Through Deterrence Strategy, Mary A. Vaughn

Department of Anthropology: Theses and Student Research

Over the last thirty years the mortality rate of the undocumented population at the US-Mexico border has risen, requiring methods distinguishing US-born individuals from immigrants to improve the likelihood of identification and repatriation through foreign consulates. Forensic anthropologists with the Pima County Office of the Medical Examiner (PCOME) have advocated for the use of the biocultural profile, combining the biological profile with factors including oral health, osteological stress, and personal effects. The biocultural profile serves two purposes, functioning both as a tool for identification and with which to document structural inequality within communities of origin.

This study examines changes in …

A Series Of Investigations On The L. Cornutus Host System And Bivesiculid Sp. Cercariae, Briana K. Zaffiro

All HCAS Student Capstones, Theses, and Dissertations

Rising global temperatures are likely modulating parasite-host interactions, yet the direction and magnitude of these changes remain uncertain. Rarely are these interactions viewed from both a host and parasite perspective. Using both CT_max, or Critical Thermal Maximum, and Clark-type polarographic oxygen microelectrodes, the physiological metabolic effects are investigated. CT_max is examined in Common Shiners, Luxilis cornutus, and is shown to be modulated by parasitism: uninfected fish had the highest CT_max average of 37.0°C, whereas heavily infected fish had decreased CT_max averaging 35°C. Prior to the parasite metabolic investigation, a suitable medium was determined, as …

Propagation And Cultivation Of Sarracenia Species, Kathryn M. Huether

Electronic Theses and Dissertations

This study evaluates the effectiveness of seed treatments on Sarracenina species to overcome seed dormancy; as well as evaluating fertilizer concentration and substrate mixture impacts on the growth of Sarracenia × wrigleyana, ‘Scarlet Belle’. The first portion of this study evaluated the effects of cold stratification and chemical treatments (smoke water and gibberellic acid) on the germination rates of Sarracenia alata and leucophylla. The primary objective was to determine whether increasing concentrations of these treatments paired with or without stratification could significantly enhance germination and serve as viable seed treatments for horticultural use. Germination did occur within all the …