Digital Commons Network^™

Cell Cycle Inhibition: Illudin S And Its Effects On Cdk2 In Ewing Sarcoma, Stephanie Diez, Natalie Flores, Alexandria Hernandez, Viviana Palacios, Terry Jo Shackleford

Cell and Molecular Methods

Ewing Sarcomas are aggressive round cell mesenchymal neoplasmas that have a high occurrence in children and young adults. CDK2 is a protein that is involved in the G1 phase of the cell cycle, which promotes the transition to the S phase. CDK2 kinase activation is mainly observed in the G1/S-phase transition.3 CDK2 binds to Cyclin proteins is responsible for entry and progression, thereby leading to maximal apoptosis activity in the S-phase. Illudin S is a drug that has been known to target cancer-specific cells, such as leukemia. Illudin S, in general are a cytotoxic metabolite that comes from plants, specifically …

Yap1 Knockdown And Panobinostat Treatment Increase Apoptotic Response And Decrease Gene Expression In Ewing Sarcoma Cells, Luna Collazo-Garcia, Alejandra Favela Santos, Madeline Torres-Salazar, Isabella Toscano, Terry Jo Shackleford

Cell and Molecular Methods

Ewing sarcoma (EWS) is considered to be one of the most aggressive pediatric malignancies, often characterized by its dysregulated gene expression driven by oncogenic fusion proteins. The Hippo signaling effector Yes-associated protein 1 (YAP1) has been known in promoting cell proliferation, survival, and therapeutic resistance in multiple cancers. However, its role in Ewing sarcoma response to treatment remains unclear. This study investigated whether YAP1 knockdown enhances the sensitivity of Ewing sarcoma cells to the histone deacetylase inhibitor Panobinostat. Ewing sarcoma, ES8, cells were transfected with a YAP1-targeting siRNA (siYAP1) or a non-targeting control (siControl) and treated with DMSO, 0.1 μM, …

Everolimus Treatment Combined With Akt1 Knockdown Increased Apoptosis And Decreased Cell Proliferation In Ewing Sarcoma Cells, Arisha Arif, Alfie Barcenez, Sophia Ruter, Nicole Vanegas-Riddick, Terry Jo Shackleford

Cell and Molecular Methods

The purpose of this study is to use the gene AKT1, due to the interest in AKT1’s role in cancer cell proliferation, and the drug Everolimus, to determine if combined targeted therapy works as a more efficient therapeutic approach. Ewing Sarcoma has been connected to chromosomal translocations and is most common in pediatric patients. It is most often treated with chemotherapy and local treatments. It may be connected to the gene AKT1 due to how it regulates cell metabolism, growth, and proliferation. The gene mTOR is similarly connected to cell metabolism and proliferation, and is inhibited by the drug Everolimus. …

Sirna Knockdown Of Rptor Reduces Rptor Expression, Increases Brd4 Expression, And Alters Proliferation And Apoptosis In Ewing's Sarcoma Cells, Sergio Cipriano, David Leavitt, Michael Olivia, Liam Valdez, Terry Jo Shackleford

Cell and Molecular Methods

Ewing sarcoma is a highly aggressive cancer that primarily affects children and young adults. Although treatment options exist, many patients do not respond effectively, showing the need for improved targeted therapies. RPTOR is a key in mTORC1 complex which regulates cell growth, proliferation, and survival. LY2874455 is a selective pan-FGFR inhibitor that targets growth factor signaling pathways involved in tumor progression, and FGFR signaling interacts with pathways such as mTOR, making it a potential target for combination therapies. We hypothesized that silencing RPTOR in Ewing sarcoma cells would disrupt mTOR signaling and alter expression of genes linked to cell survival …

Everolimus And Sirna-Mediated Mtor Inhibition Reduces Proliferation And Promotes Apoptosis In Ewing Sarcoma Cells, Dylan Calvert, Malorie Martinez, Alexa Miner, Ellis Quiroga, Terry Jo Shackleford

Cell and Molecular Methods

Ewing Sarcoma (ES) is an aggressive pediatric bone cancer characterized by rapid cell proliferation and poor prognosis, making the identification of therapeutic targets critical. One of  the mechanistic targets is rapamycin (mTOR) signaling pathway, which is critical for regulating cell growth, proliferation, and survival. Abnormal activation of the mTOR signaling pathway has been linked to the progression of ES, as it drives uncontrolled cell growth and apoptosis resistance. Because mTOR represents a promising therapeutic target for ES treatment, we hypothesized that inhibiting mTOR activity through an siRNA-mediated knockdown or through Everolimus drug treatment, would reduce cell proliferation and promote ES …

Re-Evaluating The Safety Of Erythritol: A Critical Review Of Emerging Evidence, Kaitlyn B. Kirchner

Honors Thesis

Erythritol, a commonly used non-nutritive sweetener, has gained popularity as a sugar alternative due to its low caloric value and minimal effect on blood glucose levels; however, emerging research has raised questions about its overall safety. This literature review critically evaluates current peer-reviewed evidence on the effects of erythritol on the cardiovascular and gastrointestinal systems. Findings indicate that erythritol is generally well tolerated in the gastrointestinal tract, with fewer adverse effects than other sugar alcohols due to its rapid absorption and limited fermentation, although symptoms may be dose-dependent and influenced by co-ingestion with other carbohydrates. In contrast, evidence related to …

Role Of Kif4a In The Fxa-Induced Phenotypic Modulation Of Pleural Mesothelial Cells, Sudishna Khanal

Biotechnology Theses

Pleural injuries can disrupt the organization of the pleural surface, leading to pleural remodeling and fibrosis (PF), characterized by the proliferation of myofibroblasts expressing alpha-smooth muscle actin (α-SMA) and the deposition of extracellular matrix proteins, primarily due to the mesenchymal transition of pleural mesothelial cells (PMCs) (MesoMT). It is well established that various external stimuli, such as TGF-β, thrombin, and factor Xa (FXa), induce MesoMT. Our laboratory found that myocardin, a transcriptional co-activator, was dramatically up-regulated during MesoMT, and that gene silencing of myocardin (MyoCD) diminished MesoMT. RNA sequencing data showed that FXa activated a set of genes not upregulated …

Analyzing Sun Bucks Program Experience Among Southwestern Pennsylvania Families With Just Harvest, Olivia Mae Gifford

Public Health Capstone Projects

1. Objectives: This project primarily seeks to identify the common barriers preventing vulnerable families from accessing the SUN Bucks Program. The SUN Bucks program is an optional program through the United States Department of Agriculture that works in conjunction with the state of Pennsylvania in order to provide school-aged children families with supplemental funds to supply greater nutrition during the summer months. This research supports the work of a local non-profit, Just Harvest, in order to better inform their advocacy and outreach efforts as they fight against food insecurity in Western Pennsylvania.
2. Methods: All data was collected electronically via a …

Associations Between Levels Of Physical Activity And Cognitive Health, Gemma Goldenbaum

Honors Theses

The purpose of this study is to examine the relationship between physical activity levels and executive function, specifically focusing on inhibitory control and working memory capacity. Though the benefits of exercise on brain health are well-documented, the extent of cognitive differences between sedentary individuals and elite student-athletes remains misunderstood. Eighty participants (ages 18–35) were categorized via the International Physical Activity Questionnaire (IPAQ) into sedentary, low, and advanced activity tiers. Participants completed computational assessments, including the Operation Span (OSPAN) task for working memory and Go/No-Go tasks to measure response inhibition and implicit associations. Preliminary results indicated significant differences in sedentary behavior, …

Photo Identification On Interactions Between Tursiops Trucatus And Humans To Promote The Importance Of Sustainability, Julia Bronneberg

Undergraduate University Honors Capstones

The Mediterranean Sea is one of the most biodiverse regions, and especially around the Maltese islands, a popular tourist destination where people come to enjoy the beautiful weather and blue waters. Because bottlenose dolphins (Tursiops truncatus) are the most common species people see in coastal areas, they are the primary focus of this research. We care about these bottlenose dolphins because they cannot protect themselves if we are the ones accidentally causing them harm. This study starts from the belief that if we cannot protect the ocean and marine life, then we cannot protect ourselves. We use many resources that …

Ecological Drift And Host Filtering Jointly Structure Foliar Endophytes During Ecosystem Development., Caio César Pires De Paula, Petr Macek, Milan Varsadiya, Jakub Borovec, Mehmet A. Balkan, Brett S. Younginger, Daniel J. Ballhorn, Tomáš Picek, Tomáš Hájek, Jan Frouz, Jiří Bárta, Dagmara Sirová

Biology Faculty Publications and Presentations

Foliar endophytes contribute to plant nutrient acquisition, stress tolerance, and pathogen resistance, yet their responses to ecosystem-level processes remain poorly understood. Using a space-for-time substitution design, we investigated bacterial and fungal community dynamics in the foliar endosphere of four phylogenetically distinct plant hosts across a well-characterized successional chronosequence.

A Survey Of Lichen Native To Three Of Mississippi’S National Forests, William S. Granberry Iii

Honors Theses

Lichens are a major part of the environment for telling the air quality and the impact humans have on the environment. These signs go into how many species of lichen are present in a general area, and there has been a lack of a list of lichens that call Mississippi their home. The goal of this research is to help create this list through a survey of lichen in three of Mississippi’s National Forests: DeSoto, Homochitto, and Holly Springs. These three forests are ideal because they cover differing ecosystems and are in different parts of the state, heightening the chance …

Windows Of Developmental Susceptibility To Tetrahydrocannabivarin (Thcv) Reveal Stage-Dependent Morphological And Biological Effects, Mirielle Clayton

Honors Theses

Tetrahydrocannabivarin (THCV), a minor cannabinoid found in Cannabis sativa, is gaining attention for its potential therapeutic applications, yet its effects on early development remain poorly understood. This study investigated how the timing of THCV exposure influences morphological, behavioral, and bioaccumulation outcomes in zebrafish (Danio rerio) embryos. Embryos were exposed to 2 μM THCV during discrete developmental windows (6–30, 30–54, 54–78, 78–102) and continuous 6–102 hours post-fertilization (hpf), designed to model early stages of human gestation. Behavioral analysis using a larval photomotor response assay revealed significant reductions in locomotor activity during dark phases, with the strongest hypoactivity observed …

Evolution Of Nematode Prey Chemotaxis Behavior Across Different Environments, Hailey R. Smith

Honors Theses

Climatic conditions in the near future are projected to be warmer, with more frequent and intense weather events. This will substantially alter the environments in which organisms must integrate specific information to find essential resources. Many organisms rely upon chemical signals to orient towards attractive resources through a mechanism called chemotaxis. How different environmental conditions, like temperature, impact this process will be important to understand as environments change. I conducted a laboratory evolution experiment with the model nematode Caenorhabditis elegans to see how success in chemotaxis assays changes over time when selected under different temperature regimes (normal laboratory temperature, increased …

Mechanisms Underlying Diversity In Bacterial Biofilms, Jacob David Holt

Dartmouth College Ph.D Dissertations

Bacteria commonly exist in natural environments as communities of multiple strains and species attached to surfaces and encased in an extracellular matrix of DNA, protein, and polysaccharides–termed biofilms. These biofilms contribute to diverse functions, from ocean carbon cycling via chitin degradation to pathogenic processes such as wound or implant colonization. The molecular mechanisms governing biofilm formation and composition have been well characterized in model pathogenic organisms, yet the extent to which aspects of biofilm formation contribute to the assembly and maintenance of diverse communities remains less well understood. Using microfluidics, confocal microscopy, and model biofilm-forming bacteria, this thesis details how …

Challenges And Innovations In Tennis Coach Development: International Insights From Practitioners Across Continents, Miguel Crespo, Rafael Martinez-Gallego, Christine Nash

Journal of Coach Development

Tennis is a global sport with effective coaches being essential to the continued appeal, therefore the work of the coach developers within the International Tennis Federation (ITF) is crucial. This research examined the work and aspirations of coach developers in tennis using in-depth interviews with 10 ITF coach developers. Subsequently, we developed detailed pen portraits of two ITF coach developers at different levels of experience and engagement in order to highlight any differences in work, objectives or ambition. Findings from coach developers revealed three themes: support from ITF; barriers to learning; making learning less traditional. The pen portraits added further …

Biochemical Characterization Of Luciferase In Omphalotus Olearius, James H. Martin

Honors Theses

The overall goal of this thesis was to investigate the genetic basis of bioluminescence in Omphalotus olearius, a bioluminescent mushroom. The project initially focused on growing fungal cultures under controlled laboratory conditions and using molecular techniques such as DNA extraction, PCR, and gel electrophoresis to identify the organism and isolate the gene responsible for light production. The main objective was to insert this gene into a bacterial system to confirm its function through observable bioluminescence. However, repeated difficulties in maintaining viable fungal cultures and errors in molecular procedures resulted in inconclusive results, preventing successful gene identification. Due to these …

Antibiotic Resistance Patterns In Bacterial Isolates From Mississippi Gulf Coastal Environments, Lawler F. Horn

Honors Theses

Coastal environments host diverse microbial communities that play essential ecological roles but may also serve as reservoirs for antibiotic-resistant bacteria. This study investigated patterns of antibiotic resistance in microbial isolates collected from public beaches along the Mississippi Gulf Coast. A total of 143 bacterial isolates exhibited growth and were included in antibiotic susceptibility screening. High levels of antibiotic resistance were observed, with 137 of 143 isolates showing growth on vancomycin, 124 on amoxicillin, and 102 on erythromycin. Resistance to kanamycin (19/143 isolates) and tetracycline (33/143) was less common. Multidrug resistance was widespread, with 101 isolates (70.6%) resistant to three or …

Community Composition Of Antibiotic-Resistant Bacteria On The Mississippi Gulf Coast, Marisa Martin, Colin Jackson

Honors Theses

The Mississippi Gulf Coast is an important resource for transportation, economic development, and recreation. Given its economic and cultural importance, it is crucial to maintain clean and safe conditions along the coast for public health, but other than routine beach monitoring, few studies have examined microbial contamination of coastal waters in this region. This study aimed to determine the quantity, composition, and distribution of bacteria resistant to six different antibiotics (Vancomycin, Kanamycin, Tetracycline, Ciprofloxacin, Erythromycin, or Amoxicillin) in seawater at seven public beaches along the Mississippi Gulf Coast. No bacteria resistant to Ciprofloxacin were detected, and bacteria resistant to Kanamycin …

Isolation And Quantification Of Rna From Extracellular Vesicles Derived From A549 Human Non-Small Cell Lung Cancer Cells Using Ultracentrifugation, Yousef Dimian, Vanessa Pizutelli, Dimitri Pestov

Rowan-Virtua Research Day

Extracellular vesicles (EVs) have gained attention as potential diagnostic tools due to their presence in body fluids and their ability to carry nucleic acids, including RNA. EV-derived RNA, particularly ribosomal RNA (rRNA), may reflect cellular stress and disease states, making it a promising biomarker1, 2. This project focuses on optimizing methods to isolate EVs and quantify their RNA content using human cell culture models. Differential ultracentrifugation was used to separate vesicle populations based on size, followed by RNA extraction and quantification using a high-sensitivity assay. Initial experiments using media from three 150 mm culture dishes yielded no detectable RNA, whereas …

Optimization Of Refolding, Purification, And Analytical Characterization Of Proinsulin(F25d) Soluble Antigen Array Immunotherapy, Abigail Richardson

McKelvey School of Engineering Graduate Student Theses & Dissertations

Type 1 Diabetes (T1D) is characterized by autoreactive immune responses to insulin and its precursor, proinsulin. Soluble antigen arrays (SAgAs) displaying the metabolically inactive proinsulin variant, proinsulin(F25D), represent a promising antigen‑specific immunotherapy candidate for T1D. However, the production of these platforms is hindered by substantial practical challenges, including protein aggregation, inefficient refolding, and the lack of sensitive analytical tools to verify quality and dosage. This work establishes an integrated, scalable pipeline for the purification, conjugation, and quantification of a proinsulin(F25D) SAgA. First, we optimized the refolding of recombinant methionine-proinsulin(F25D) (M-PI(F25D)) from inclusion bodies using controlled buffer systems and created a …

The Longevity Of Dna From Bloodstains And Its Forensic Implications, Nadia Walter

Senior Honors Theses

Human DNA is valuable forensic evidence, helping to solve a wide range of cases. It is often sourced from biological fluids found at a scene such as blood, saliva, semen, and urine. Many different factors can influence the longevity of DNA sourced from bloodstains, including the type of substrate, storage conditions, and weather. The quality of a DNA sample from a bloodstain found at a crime scene may influence its admissibility in court.

Original research was done on a surgical table from the last major battle of the American Civil War, extracting DNA from latent bloodstains. Some full profiles were …

Quantifying Genetic Diversity In Nematode Feeding Rate, Jacob King

Honors Theses

Understanding how populations persist under changing environmental conditions is a central question in ecology and evolutionary biology. Variation in traits related to resource acquisition may play a key role in determining population survival when resources are limited or fluctuate over time. In this study, I investigated how variation in feeding-related traits influences population persistence using a combination of computational modeling and laboratory experiments. I developed a stochastic, individual-based consumer–resource model to simulate population dynamics across varying levels of trait variance and resource regimes. Across 60,000 simulated populations, increased trait variation consistently reduced extinction risk and extended persistence time, although the …

Faith, Science, And Choice: Vaccine Attitudes Among Religious University Students, Jamie Jensen

ScholarsArchive Data

These data comprise a Q-methodology study conducted at Brigham Young University among undergraduate students to determine vaccine attitudes in relation to religious and political ideologies. It includes raw sort data, templates for running Q-methodology, and interview transcripts.

Herbularyo (Folk Healer): An Herbal Medicine Card Game, Maryjane T. Magsino, Inah Marie Q. Rivera, Armando M. Guidote, Genejane M. Adarlo

Chemistry Faculty Publications

Herbularyo, a card game teaching about herbal plants (HPs), was created and evaluated as a viable educational card game based on Tuomisto and Aksela’s criteria. A quasi-experiment was performed on 81 Grade 8 students from a private high school and 50 Grade 11 from a public high school. Independent t-test revealed that the control group (CG) and experimental group (EG) (p=0.386), and EG1 (played ≤5) and EG2 (played >5) (p=0.681) are not comparable with each other. Paired t-test showed EG performed better (p=0.001) than CG (p=0.901) whereas ANCOVA showed that playing Herbularyo improved students’ information retention (p=0.006). EG1 and EG2 …

Optimizing Expression Conditions For The Overexpression Of Iron Regulatory Protein Bol2 From C. Glabrata, Jade Ikahihifo-Bender, Dr. Caryn Outten, Mrs. Evan Talib

Caravel Undergraduate Research Journal

Due to its unique ability to serve as both an electron donor and acceptor, iron is utilized as a co-factor for many biological processes, including electron transfer, oxygen binding, and vitamin synthesis. Iron is also a key factor during fungal infections as the human host and invading pathogens battle over limited iron pools. The primary iron-responsive transcription factor Aft1 in the opportunistic pathogenic yeast Candida glabrata responds to iron deficiency by activating expression of iron acquisition genes. However, the mechanisms for sensing intracellular iron levels and regulating Aft1 activity in response to iron are unknown. The C. glabrata iron regulation …

Injuries In Youth Football: A Two Year Descriptive Study Of Local South Carolina Football Leagues, Marie Morrissette, Dr. Jim Mensch, Dr. Daheia Barr-Anderson

Caravel Undergraduate Research Journal

There has been an increase in the media attention surrounding retired National Football League players and the lasting effects from previous injuries.  With more than 20 million children under the age of 17 participating in competitive sports, the attention in the lasting effects of injuries in professional players has trickled down to the youth level.  Of the approximately 20 million children participating in competitive sports, the largest portion of the participants is playing youth football.  The University of South Carolina Athletic Training Department is partaking in a multisite research study conducted by the Datalys Center to track the injuries of …

A Review Of Video Game Effects And Uses, David Corso

Caravel Undergraduate Research Journal

Video games are versatile in their form and function. Whether they’re training doctors or offsetting Alzheimer’s disease, video games can exercise both mental and physical features, train specific skills for a number of jobs, educate players about events and phenomenon in our world, research and understand natural human processes, and they can be designed for a number of other purposes. Multiple studies have demonstrated the effects of video games on physiological and cognitive levels—positive, negative, and everything in between. This paper identifies various physical and psychological effects that video games produce, and it suggests ways in which video games can …

Teaching Exercise: A Survey On Educational Aids And Self-Efficacy, Joshua I. Eaton, Lucas Morgan, Matthew Hermes

Honors College Theses

HEPs are an important part of therapeutic treatment because they allow for greater exercise stimulus than can be achieved from supervised exercise alone. 94.7% of physical therapists reported prescribing HEPs to their patients. However, patient adherence to these plans can be as low as 35%. Lack of HEP adherence likely leads to sub-optimal clinical outcomes in physical therapy patients. Self-efficacy is improved in patients by supplementing HEPs with instructional aids, especially in the form of videos instead of pictures or descriptions, because they act as a form of vicarious experience. It is possible that improving self-efficacy through vicarious experience could …

I Lost My Number, Can I Have Yours? Body Image Satisfaction With Sexually Dimorphic Traits Is Associated With Men's Dating Anxiety And Confidence, David A. Frederick, Tania A. Reynolds, Nikoline L. Smart, Justin R. Garcia, David A. Puts

Psychology Faculty Articles and Research

Evolutionary scientists posit that sexual selection processes caused males to develop sexually dimorphic traits to aid them during intrasexual competition and increase their likelihood of being chosen as mates. Evolutionary social sciences have extensively examined women's preferences for traits such as muscle mass and voice pitch, reasoning that these traits serve as cues of men's ability to provide direct benefits (e.g., resources) and indirect (genetic) benefits passed on to offspring. At the same time, body image researchers have extensively focused on the social causes and consequences of men's dissatisfaction with their muscularity and body fat. We connected these two research …