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Studies Of Teratogenic Viruses: Minute Virus Of Mice And Colorado Tick Fever Virus, Robert Eldred Harris

Theses and Dissertations

Studies were conducted in DUB/ICR mice with minute virus of mice (MVM), a single stranded DNA virus, and Colorado tick fever (CTF) virus, a double stranded RNA Virus, to determine if CTF virus had teratogenic potential and to characterize further MVM and its teratogenic effects. MVM is a known teratogen, whereas CTF virus had never been investigated previously for teratogenic potential.

In characterization studies of MVM infectivity, the following were determined: (a) Rat embryo cells proved to be superior to six cell lines in assaying for MVM infectivity; (b) MVM infectivity could be inactivated with common laboratory disinfectants, ultraviolet radiation, …

A Medicated Dry Foam For Local Therapy In Infected Burns, Patrick Nicholas Catania

University of the Pacific Theses and Dissertations

Local burn therapy usually includes the application of medicated ointments and dressings in order to minimize and eradicate infection. As this approach to local therapy may present problems regarding application and removal techniques, it was the purpose of this study to formulate a topical dosage form that may be applied to denuded areas of the skin without inunction. Using the concept of topical films, a dosage form was formulated that is highly water soluble, flexible , and adherent to both denuded and intact areas of the skin.

Some Aspects Of Mercury Accumulation By The Purple Shore Crab Hemigrapsus Nudus Dana (Crustacea: Decapoda), Ralph Gene Swanson

University of the Pacific Theses and Dissertations

The present study has attempted to investigate some of the more basic questions concerning the toxicity and accumulation of mercury in the purple shore crab, Hemigrapsus nudus. This rocky intertidal denizen is found in abundance along the entire Pacific coast of the United States from Sitka, Alaska, to the Gulf of California where it dominates the mid-tide pool region. Local specimens were collected just north of the Pacific Marine Stations, Dillon Beach, Marin County, California. The animal is hearty and can be kept in a state of good health for long periods of time in the laboratory with minimum care …

A Study Of The Effects Of Changing Environmental Salinity Upon The Prolactin Cells In A Freshwater Teleost, Xiphophorus Maculatus (The Platyfish), Seymour Holtzman

Dissertations, Theses, and Capstone Projects

No abstract provided.

Propylene Oxide Sterilization Of Microbiological Media In The High School Classroom, Marvin A. Andersen

Dissertations and Theses @ UNI

The effective use of propylene oxide as a sterilizing agent has been demonstrated by many workers. Its use in the high school has been suggested but not studied. The intent of this work was to ascertain the feasibility of such an application. Preliminary experiments were performed to determine the most effective concentration of propylene oxide exposure in a closed system to achieve sterilization. For heavily contaminated plates a concentration of 2.0 ml/L of propylene oxide for twenty-four hours was found to be most effective. Comparison tests were made of both propylene oxide and pressure cooker sterilized nutrient agar. Sources of …

N-Methyl-N’-Nitro-N-Nitrosoguanidine Induced Dominant And Recessive Mutations In Zea Mays, Frank Charles Janca

Masters Theses

No abstract provided.

An Investigation Of The Froth Flotation Process As A Means Of Concentrating Pulp And Paper Mill Sludge, Merwin Lee Miller

Masters Theses

No abstract provided.

The Recovery Of Portage Creek In Vicksburg, Michigan, Michael Henry Herbert

Masters Theses

No abstract provided.

Effects Of The Exogenous Hormone Cortisone Acetate On Drosophila Virilis Larvae, Margarita Midelfort

Bachelors’ Theses

Recent experiments published in the literature have questioned the importance of the appearance of chromosomal puffs during larval development in Dipterans since Sciara larvae fed the vertebrate hormone cortisone apparently show no puffs without impairment of normal development. We have used Drosophila virilis larvae to test these results because distinct morphological criteria can be used to assess the developmental age of third instar larvae and predict the appearance of a specific puff. We have found that even though ingestion of cortisone acetate by D. virilis larvae causes a marked delay in their development the chromosome puffs appear at the expected …

Herpetomonas Megaseliae Sp. N. (Protozoa: Trypanosomatidae) From Megaselia Scalaris (Loew, 1866) Schmitz, 1929 (Diptera: Phoridae), Pierre M. Daggett, John J. Janovy Jr., Norman R. Dollahon

John J. Janovy, Jr. Publications

A new intestinal trypanosomatid was found parasitizing Megaselia scalaris. Forms with posterior kinetoplasts occurred in both natural infections and cloned cultures, but not in experimental infections. Average size and morphological variation were reduced in experimental infections with cloned stocks, compared to natural infections. A growth comparison was made between Herpetomonas muscarum and H. megaseliae. Differences in day of peak population, maximum numbers, and total time of survival were observed in culture. Attempts to infect Drosophila melanogaster with H. megaseliae were unsuccessful.

Measurement Of Haversian Bone Remodelling By Means Of Tetracycline Labelling In Rib Of Rhesus Monkeys, Charles C. Schock, Frank R. Noyes, A. R. Villanueva

Henry Ford Hospital Medical Journal

Quantitative histologic measurements of haversian bone remodelling were made of mineralized rib cross sections in 13 healthy rhesus monkeys. With the aid of previous tetracycline labelling, coupled with the Villanueva Osteochrome stain, Frost's quantitative bone analysis revealed that: a) the mean haversian bone formation rate is 0.359 z 0.186 mm²/mm²/yr; b) the mean number of osteoid seams/mm² is 4.03 ± 1.64; c) the mean appositional rale is 1 33 ± 0 23 microns/day while the radial closure rate when all the labelled systems are corrected is 0.42 r 0.080 mm/year; d) the mean circumference of …

Mites Of The Family Laelapidae In Venezuela (Acarina: Laelapidae), Deane P. Furman

Brigham Young University Science Bulletin, Biological Series

This publication presents the results of an extensive survey of symbiotic laelapid mites of vertebrates from Venezuela. Over 40,000 vertebrates were examined from a wide variety of life zones and localities. The laelapid fauna represented includes 1 new genus, Hymenolaelpas, and 7 genera previously described: Laelaps, Mysolaelaps, Tur, Steptolaelaps, Neoparalaelaps, Gigantolaelaps, and Androlaelaps. The genera are represented by 51 species and subspecies, of which 8 are described as new. Previously unknown males and immatures of several species are described. Gigantolaelaps versteegi ( Oudemans ) is redescribed from type material; G. wolffsohni (Oudemans) is redescribed from Oudemans’ original material; G. peruviana …

Current Tests For Tubal Patency: Their Study And Comparison, Asghar Afsari, Robert J. Thompson

Henry Ford Hospital Medical Journal

Two hundred patients have been reviewed in whom culdoscopy or laparoscopy was performed for infertility study. During these two procedures, methylene blue was injected through an endocervical cannula for detection of tubal patency under direct visualization. When compared with 89 patients tested by utero-tubal insufflation and hystero-salpingography, results indicated that direct visualization of the tubes and dye hydrotubation appears to be the most reliable method. HSG proved to be the second most reliable method with a failure rate of 19.2%. The least accurate procedure in this study was UTI with CO₂ gas, where the failure rate was 24.7%. The …

The Effects Of Different Gases On Excystation Of Coccidian Oocysts, James B. Jensen

Theses and Dissertations

Excystation of coccidian oocysts is a diphasic process where CO2 and suitable reducing agents cause changes in oocyst wall permeability (phase I) allowing the entrance of trypsin and bile which activate the sporozoites (phase II). This project was an indirect study of the mechanism of CO2 action by the substitution of NO, NO2, N2O, H2S, SO2 , CH4, NH3 and 8M urea in place of CO2. Changes in oocyst wall permeability of Eimeria stiedae, E. bovis and E. tenella were determined by incubation with the reagents and cysteine HCl followed by treatment with trypsin and bile to initiate activation of …

Bionomics And The Influence Of Diet Upon The Maturation Of Orchopeas Leucopus (Baker), Lynden Phillip Baum

Theses and Dissertations

Knowledge of the bionomics of plague vectors, es-pecially factors which influence life history, host pre-ference, and fluctuations of flea populations, are basic to an understanding of plague ecology. Orchopeas leucopus (Baker), an ectoparasite of cricetid rodents such as Peromyscus maniculatus (Wagner), and Peromyscus truei (Baird), is a vector of plague in the western United States (Holdenried and Morlan, 1955). In a study of nine biotic corrnnunities in the Utah portion of the Great Basin, P. maniculatus was the most abundant rodent in seven of the biotic corrnnunities (Olsen, 1966). Because of the ubiquitous nature of P. maniculatus an interchange of …

Lipids Of Fungal Spores, Identification And Metabolism, Jim L. Bushnell

Theses and Dissertations

The lipids of the spores of the Basidiomycetes: Ustilago bullata, Lycoperdon perlatum and Calvatia gigantea were investigated. The spores of U. bullata were found to have hydrocarbons ranging from C_9 to C_24, methyl esters of fatty acids ranging from C_14 to C_24, and free fatty acids from C_14 to C_24. The spores of L. perlatum were found to have few hydrocarbons (C_22 being the predominent one); no methyl esters were detected and the fatty acids were from C_15 to C_18. Long chain aldehydes (C_30) were also detected in low concentration. At the ultrastructural level the Lycoperdon spores were found to …

The Development And Testing Of A Fire Simulator Exercise, Don A. Clymer

Electronic Theses and Dissertations

A forest fire simulation exercise was developed for the purpose of supplementing forest fire instruction, presenting students with decision-making situations encountered on forest fires, and introducing them to the fire simulator at Stephen F. Austin State University. The final exercise was developed after preliminary work and development of a more simple exercise. The problems with equipment, recruitment of simulator personnel, and the development and execution of the simulator exercises are discussed. Scheduling adequate time for practice, and providing adequate personnel training were found to be the major obstacles in simulator use. Future fire simulator use at SFA will be dependent …

Rna Metabolism In Escherichia Coli During Bacteriophage T4 Infection, Amino Acid Starvation And Complete Inhibition Of Protein Synthesis, Elizabeth Anne Craig

Arts & Sciences Graduate Student Theses and Dissertations

Three classes of RNA are made in exponentially growing E. coli cells: messenger RNA (mRNA), ribosomal RNA (rRNA) and transfer RNA (tRNA). mRNA possesses the characteristics which were first proposed by Jacob and Monod (1961) as necessary for an intermediary in the transfer of information from DNA to protein.

The Interaction Of Promazine With Selected Macromolecules And A Membrane Utilizing Absorption And Emission Spectroscopy, Jess D. Keeler

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The association of promazine-HCl with bovine serum albumin, ammonium detergent micelles, and hemoglobin-free-rabbit-erythrocyte membranes were measured using the changes in the absorption and the emission of the interactants plus the fluorescent probe 1-anilino-8-naphthalene sulfonate. The binding of a promazine to serum protein and membrane did not alter the emission of the drug. However, the binding did produce a measurable quenching of the membrane and protein fluorescence. The quenching reaction was pH sensitive for both the protein and the membrane. Promazine binding to serum protein produced a hyperchromic change in the drugs absorption spectrum which paralleled the quenching reaction. The binding …

The Effects Of Temperatures And Chemicals On The Oocysts Of Eimeria Stiedae, Charles Larry Keehner

Theses and Dissertations

A study was made of the effects of temperatures and chemicals on the unsporulated and sporulated oocysts of Eimeria stiedae. In general, oocysts were affected by temperatures lower than -10 C and above +39 C, while normal development occurred between these temperatures. Unsporulated oocysts were more tolerant to temperature extremes, as they survived up to 8 days at 39 C and 12 days at -28 C. Sporulated oocysts were killed after 24 hours storage at 39 C and 4 days at -28 C. Oocyst resistance to low temperatures was not improved if unsporulated and sporulated oocysts were conditioned before and …

Louisiana Agriculture Magazine, Summer 1972, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Quantitative Growth Relationships Of Microcoleus Vaginatus (Gom.) Ex Drouet And Alcaligenes Sp. In A Sucrose-Salts Medium, James Rexford Brownell

Master's Theses

The blue-green alga, Microcoleus vaginatus (Gom.) ex Drouet 1968, and a bacterium, Alcaligenes sp., collected from the same habitat, were cultured together and separately in mineral salts media with and without sucrose. Growth of axenic M. vaginatus was stimulated by sucrose. In association with the bacterium in sucrose-containing media algal growth was suppressed, while in inorganic media it was stimulated. Factors involved in this algal-bacterial system probably included competition for the carbon source, changes in pH, and accumulation of cellular waste and lytic products.

Annual Report And Membership List 1971-72, Iowa Academy Of Science

Iowa Academy of Science Documents

Contents:

Minutes of the Annual Business Meeting --- 4
Report of the Executive Secretary --- 8
Report of the 84th Session --- 14
Report of the Social Implications Committee --- 16
Report of the Finance Committee --- 18
Treasurer's Report --- 20
Report of the Librarian --- 22
Report of the Recognition and Awards Committee --- 23
Report of the Student Programs Committee --- 26
Report of the Articulation Committee --- 29
Report of the Resolutions Committee --- 31
Report of the Membership Committee --- 32
Constitution, Bylaws, and Articles of Incorporation --- 33
Constitution --- 34
Bylaws --- 38 …

Phyletic Trends In Certain Lineages Of Quaternary Mammals, C. Bertrand Schultz, Lloyd G. Tanner, Lary D. Martin

Bulletin of the University of Nebraska State Museum (1924–2023)

Evolutionary trends in certain carnivores, rodents, bovids, and proboscideans are examined. Chronoclines are demonstrated for muskrats, beavers, mammoths, and bison. The Pliocene-Pleistocene boundary and Quaternary climatic fluctuations are also discussed.

The University of Nebraska State Museum contains a large stratigraphically controlled collection of Pleistocene animals. This collection provides a unique opportunity for the study of phyletic trends in certain lineages of mammals. Although a few phylogenetic lineages have been proposed (Schultz and Frankforter, 1946; Hibbard, et al., 1965), this has not been done for the vast majority of Pleistocene mammals. However, there is much more data available than this would …

The Effect Of Various Nutrients On The Sporulation Of Trichophyton Rubrum, P. Jerry Austin

Loma Linda University Electronic Theses, Dissertations & Projects

Trichophyton rubrum was grown on a chemically defined media in an effort to find a substance that would give the maximum production of macroconidia. Twenty-one amino acids were tested and about one half of them were active in stimulating macroconidial formation. Eight purines and pyrimidines were also tested but none of them gave more than scanty sporulation. The base media used was Czapek solution agar (modified): the saccharose was replaced with an equal amount of glucose. The various nutrients were tested at two concentrations, 1.25 mg/ml and 0.25 mg/ml. Yeast extract and peptone were also included in the study since …

The Germfree Culture Of Three Species Of Triatominae: Triatoma Protracta (Uhler), Triatoma Rubida (Uhler), And Rhodnius Prolixus Stål, Stephen A. Nyirady

Loma Linda University Electronic Theses, Dissertations & Projects

Triatoma protracta protracta, Triatoma riibida rubIda, and two strains of Rhodnius prolixus were successfully reared germfree into the fourth generation. Triatominae eggs were disinfected externally with iodine and the nymphs reared in vinyl plastic germfree modules. Germfree mice were used as the host. This is the first report of Triatominae having been reared in a germfree environment. These bugs were never exposed to bacteria internally or externally to the fourth generation.

Comparisons of conventional control bugs with replicate germfree groups in the first two generations revealed but slight differences in developmental periods. The mean adult longevity of all …

Spiny Lobster Mariculture In The State Of Florida, W. Richard Boehmer

Marine Affairs Theses and Major Papers

The purpose of this paper is to introduce the reader to the Florida spiny lobster, Panuluris argus; to show the economic potential for the mariculture of this species by exposing value and size of the U. S. spiny lobster market; to present Florida’s legal provisions for mariculture and how spiny lobster farming would be acceptable; to indicate the biological considerations for the rearing of spiny lobster larva and postlarva; and to introduce a general plan for the mariculture of spiny lobster, that considers the legal provisions of leasing wetlands in Florida and incorporates the biological considerations of spiny lobster.

A Survey Of The Incidence Of Salmonella Among Small Mammals In Central New Mexico, Robert S. Stinson

Biology ETDs

A twelve-month survey of the incidence of Salmonella among small mammals was conducted in central New Mexico, utilizing a direct fecal plating on both selective and enrichment media. Collections were made at the following locations: Albuquerque, Bernardo, Cuba, Isleta Indian Pueblo, Juan Tabo Park, and Moriarty. Locations selected characterize three major types of terrain: mountain, mesa, and river valley. The predominant genera caught in the mountains, in order of their frequency, were Peromyscus, Dipodomys, and Neotoma. The predominant genera caught on the mesas, in order of their frequency, were Peromyscus, Spermophilus, and Dipodomys. The predominant genera caught long the Rio …

Purification And Characterization Of A Protease From A Lamb Gastric Extract Used For Cheese Flavor Improvement, Ramjibhai V. Chaudhari

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An assay for catheptic activity of lamb gastric tissue extract has been proposed which involves the use of a pH 3.5 hemoglobin substrate following activation of zymogens at pH 2.0, 25C for 30 min.; and inactivation at pH 8.0, 40C for 30 min thereby eliminating the effects of pepsin and rennin. Cathepsin was isolated and purified by ammonium sulfate fractionation, acetone precipitation and gel filtration. The purified cathepsin represented approximately 50 fold increase in specific activity over the original extract and a recovery of 15% of the original activity. Degree of purity was monitored by isoelectric focusing in polyacrylamide gels. …

Detection And Quantitation Of Staphylococcus Aureus Deoxyribonuclease In Cheese, Cyril Newell Maughan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A specific method has been developed for the extraction and measurement of staphylococcal nuclease in cheese in which Staphylococcus aureus has grown. Ten grams of cheese sample were homogenized with ninety milliliters of pH ten buffer for three minutes. Ammonium sulfate fractionation was used and a forty to eighty percent fraction was collected and concentrated using ultrafilters. The nuclease activity was determined using a toluidine blue deoxyribonucleic acid agar slide method and a spectophotometric method. The DNA agar slide method was used to compare staphylococcal growth with nuclease production in cheese under varying conditions. When Staphylococcus aureus plate counts indicated …