Digital Commons Network^™

Blue-Green Algal (Cyanophyta) Toxins, Charles P. Mason

Journal of the Minnesota Academy of Science

In the past toxin production by blue-green algae has been demonstrated by feeding large quantities of algae to vertebrates and observing symptoms leading to animal death. It was the purpose of this investigation to develop a more convenient way to test for the presence of an alga toxin using instead of vertebrates, other algae and those organisms with which it lives and competes in fresh water habitats. Using axenic algae cultures and three different agar media MBL, DAA and NA, the latter to check for bacterial contamination, algae were streaked on the agar surface in petri dishes to form a …

Characterization And Identification Of An Isolate Of Halobacterium From Soda Springs Mojave Desert, Abayomi Odubela

Theses Digitization Project

No abstract provided.

Comparison Of Tolerance To High Moisture Conditions Of The Soil Among Crop Plants: Studies On The Comparative Plant Nutrition [English Translation], Toshiaki Tadano, Kiyokazu Kirimoto, Isao Aoyama, Arika Tanaka

Publications

Tolerance to high moisture conditions in the soil was compared for eighteen crop species, and factors causing differences among species were studied: 1) When the moisture content of a soil was kept high, the soil air contained 10.5 - 17.5% of O2 and 5.0 - 9.8% of CO2, and the soil solution was not very high in Fe, Mn or NO2-N. 2) Cruciferae, Solanaceae, sugar beet, field bean, pea, cucumber and Chrysanthemum coronarium were very susceptible to this condition, but maize, soybean, and onion were tolerant. Rice grew better under this condition than under ordinary upland conditions. 3) Tolerance to …

Soil Science Research Report - 1979

Soil Science Research Reports

Corn Experiments

Nitrogen Rates on Corn ................ Section 1

Anhydrous Ammonia and N-Serve for Irrigated Corn ................ Section 2

Placing Ammonia and APP Together for Irrigated Corn Production ................ Section 3

Nitrogen Rate and Source Studies for Corn Grown in an Ecofallow Rotation ................ Section 4

Comparison of Standard Nitrogen Fertilizers with Delayed Release and Nitrification Inhibiting Materials for Irrigated Corn on Sharpsburg/Bu1ter ................ Section 5

Nitrogen Balance Under Irrigated Corn Using ¹⁵N-Depleted Fertilizer ........... Section 6

Improving the Efficiency of Fertilizer N Use by Irrigated Corn ................ Section 7

Effect of Fertilizer Phosphorus Placement on Corn Yields in …

Probing Mitotic Chromosome Structure And Arrangement Using Micromanipulation, Bruce R. Korf

Student Theses and Dissertations

The Indian deer, (Muntiacus muntjak), has the lowest known diploid chromosome number (seven in the male) for a mammal. Mitotic chromosomes can be removed from cells either by extraction with microneedles or by inducing chromosome expulsion with a drop of silicone oil applied to the cell. The entire diploid complement is removed by virtue of interconnecting fibers among the chromosomes. The chromosomes are brought to the surface of the cover slip for analysis, where each can be identified morphologically with phase contrast optics. The chromosomes are sticky and extensible, and parts of chromosomes can be dissected by cutting with microneedles. …

Studies On Chloroplast Protein Synthesis In Vivo And In Vitro, Karl Stanley Matlin

Student Theses and Dissertations

Protein synthesis in the Chlamydomonas reinhardtii chloroplast was investigated by in vivo and in vitro approaches. The site of synthesis of chloroplast proteins was examined to determine how many proteins were synthesized inside the chloroplast. Cells were labeled in vivo with [³⁵S]-sulfate in the presence of either. chloroplast or cytoplasmic protein synthesis inhibitors, and fractionated into soluble proteins, thylakoid membranes, and chloroplast ribosomal subunits. The use of high specific activity sulfate and improved gel electrophoretic techniques were key features of this study because they permitted minor chloroplast products to be detected for the first time. It was determined …

Chromosomal Replicative Forms Of P1 And P1dlac During The Lytic Cycle, John W. Bornhoeft

Dissertations

No abstract provided.

Fire-Decay: Interactive Roles Regulating Wood Accumulation And Soil Development In The Northern Rocky Mountains, A. E. Harvey, M. J. Larsen, M. F. Jurgensen

Aspen Bibliography

Decay and fire play interactive roles in recycling wood and other organic materials in forest ecosystems, and contribute to the development of high quality soils in the Northern Rocky Mountains. Decayed wood, charcoal, and other decomposed organic matter are the principal media for ectomycorrhizal and nonsymbiotic nitrogen fixing microbes. The activities of these microbes are critical to the growth of forest trees. The balance between decay and fire, as it affects the amount, distribution, and type of organic matter, controls the ability of forest soils to support the growth of trees.

A Comparison Of Cheddar Cheese Yields And Composition Using Conventional Bulk Set Cultures And Superstart Concentrated Cultures, William J. Kipp

Electronic Theses and Dissertations

The cheese industry has become by far the largest user of milk for manufacturing. In 1978, cheese production utilized a fourth of the total market supply of milk, up from less than an eighth in 1960 (4). Rising consumer incomes and changing lifestyles and eating habits have increased cheese consumption and sales by 60% since 1960 (21). In the last ten years, annual per capita consumption of cured cheese has increased from 11.6 lb to 17.1 lb (45). Economists state that the cheese market will continue to expand through the 1980's (7, 21, 68). Manufacturing cultured dairy products can be …

Electrophoretic Analysis Of Blood Serum Proteins In Three Species Of Water Snakes (Genus Nerodia), Phyllis J. Garnett

Journal of the Arkansas Academy of Science

Serum from three species of water snakes (Nerodia rhombifera, N. erythrogaster and N. fasciata) from one geographic region was analyzed electrophoretically on cellulose acetate, and anodic mobility and relative concentration of the fractions were determined by a recording densitometer with an automatic integrator. Classification of fractions was based on mobility (Rf, values), and for identification purposes, bands were labeled in order of decreasing mobility (albumin and alpha₁, alpha₂, alpha₃, beta₁, beta₂, gamma₁, and gamma₂ globulins). Seven fractions were identified in each species with alpha₃ being absent from N. rhombifera and N. erythrogaster, and only one gamma fraction was observed in …

Studies Of The Lysogenic Response As A Function Of Multiplicity Of Infection Of Lambdoid Bacteriophages, Mark Floyd Fedyk

Masters Theses

The lambdoid bacteriophages λimm^λ, λimm⁴³⁴ and λcI are ideal subjects for the study of gene product interactions at the molecular level due to their closely related genetic makeup. Previous work in this laboratory has indicated the possibility that phage λimm is sensitive to phage λimm⁴³⁴cI gene product and that phage λimm⁴³⁴ is sensitive to phage λimm^λcI gene product. The purpose of this research was to determine if the frequency of lysogeny for phage λimm^λ is affected by the multiplicity of coinfection with the λimm⁴³⁴ phage. By comparing the graph of …

The Biodegradation Of Crude Oil By Bacteria Isolated From Brine Pits, Larry Williams

Masters Theses

The biodegradation of crude oil by bacteria was studied. Hydrocarbon-utilizing bacteria were isolated from sixteen brine pits from an oil producing region of Clark County, Illinois. The bacteria were capable of growth from the vapors arising from crude oil as a sole carbon source. Some strains of bacteria were capable of emulsifying crude oil, apparently through the production of an emulsifying agent(s) that was found to be heat stable and cell bound but which can be partially stripped from the cell by autoclaving. The bacteria appear to belong to the genus Arthrobacter. The chemical environment of the pits was …

The Effect Of Sodium Hexametaphosphate On Cottage Cheese Yields, Stephen T. Dybing

Electronic Theses and Dissertations

A major concern in the cottage cheese industry is the inefficient use of the available milk proteins. Currently, the methods of cottage cheese manufacture convert an average of only 74.9% of the milk proteins into cottage cheese, while the remainder are removed in the by-product whey (43, 56). As protein is the main determinant of both the yield and nutritional value of cottage cheese, if 25% of the available protein is lost to the whey, then potential cottage cheese yield and nutrition have been lost as well. The proteins lost in the whey are mainly -lactalbumin and β-lactoglobulin, which remain …

Mouthparts Of Orius Insidiosus (Say), Hemiptera-Heteroptera : Anthocoridae, With Emphasis On The Stimulus For Biting Man, Mark Norbert Wisniewski

Master's Theses

No abstract provided.

Microelectrophoresis As A Means Of Comparing Acid Phosphatase Isoenzymes In Aging Campanularia Flexuosa, Paul Joseph Meus

Master's Theses

No abstract provided.

The Effects Of Ph On Chloride Flux In Fundulus Heteroclitus, Timothy Alton Washburn

Master's Theses

The effects of increasing hydrogen ion concentration on rate of chloride influx and efflux were studied in the killifish, Fundulus heteroclitus, adapted to fresh water. Because increasing hydrogen ion concentrations have been shown to inhibit sodium influx and because evidence exists to support coupling of chloride transport with sodium transport, the present study was undertaken to determine what effect pH would have on rate of chloride flux. Chloride efflux rates were studied after the addition of sodium bicarbonate to the medium. No significant pH-dependent changes in efflux rate were observed, except at very low pH. Influx rates were significantly changed, …

Tritium (3h) Radiolabeling Of Protein A And Antibody To High Specific Activity: Application To Cell Surface Antigen Radioimmunoassays, Ronald L. Wilder, Catherine C. Yuen, Subbarao Bondada, Virgil L. Woods, Cornelius B. Alexander, Rose G. Mage

Microbiology, Immunology, and Molecular Genetics Faculty Publications

Staphylococcal protein A and several different immunoglobulins have been radio-labeled to high specific activities (> 106 cpm/pg) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.

Nebraska Rainbow Trout, Nebraska Game And Parks, G. Zeurlein, L. Hesse, J. Seeb, L. Wishard

Nebraska Game and Parks Commission: Publications

Chapter 1 Lethal and Preferred Temperatures of Lake McConaughy Rainbow Trout Versus Domestic Strain Rainbow Trout By R. Vancil, G. Zuerlein and L. Hesse

Chapter 2 A Nitrifying Filter-Cooling Condenser System for Total Water Re-use in Cold-Water Fish Holding or Rearing Applications By L. Hesse, G. Zuerlein and R. Vancil

Chapter 3 Biochemical Genetic Analysis of Two Strains of Nebraska Rainbow Trout By J. Seeb and L. Wishard

The self-sustaining rainbow trout population living in Lake Mcconaughy and the North Platte River tributaries is unique to the Great Plains region. The population spends most of its adult life in the …

The Biodegredation Of Vehicular Waste Petroleum In The Roadside Environment, Jesse W. Johnson

Retrospective Theses and Dissertations

Bacteria from dry and wet roadside environments were examined for the ability to degrade hydrocarbons. The kinds and numbers of bacteria observed were similar to those reported in other petroleum contaminated environments. Surface soils (top 2.5 cm) immediately adjacent to the highway pavement and the sediments of shallow drainage ditches contained the highest concentrations of petroleum degrading bacteria (9.8 x 107 CFU/g). Concentration and species diversity of petroleum degrading bacteria decreased with distance from the highway pavement. Chromatographic analysis of highway stormwater runoff and the soil in close proximity to the highway indicated the presence of complex hydrocarbon mixtures of …

A Filamentous Bacterium On The Brine Shrimp And Its Control, Mobashir A. Solangi, Robin M. Overstreet, Ann L. Gannam

Gulf and Caribbean Research

A strain of a colorless, filamentous bacterium (tentatively identified as Leucothrix mucor) heavily infests the brine shrimp, Artemia salina. Its ultrastructure, unlike that of some other strains, does not reveal a distinct middle layer between its outer cell wall layer and cytoplasmic membrane, irregular blebs extending from the cell layers, or an external sheath. An entire infestation, represented as a mat of the bacterium with associated debris and microorganisms, sloughs from the shrimp when exposed to a variety of treatments. Primarily because most effective treatments are toxic to the shrimp, 100 ppm terramycin provides the treatment of choice.

Root Anatomy Of Fine Species Of Abronia From Southern California And Its Ecological Implications, Jan Webster

Theses Digitization Project

No abstract provided.

Antiviral Activity Of Peritoneal Exudate Cells Of Mice Against Infection With Herpes Simplex Virus Type 2, Margaret Byrd Mcgeorge

Theses and Dissertations

Within the past decade, there has been increasing evidence which suggests that herpes simplex virus type two (HSV-2) is associated with cervical carcinoma. Because cancer of the cervix ranks as the second most common malignant disease of women, with 35 thousand new cases and 10,000 deaths per year, further investigation is warranted to determine if there is a causal relationship (Goldberg, 1976). Currently, most of the evidence which correlates HSV-2 with cancer of the cervix has been, for the most part, indirect: (i) there is an increased incidence of cervical anaplasia in women with cytologically detectable genital herpes infection, (ii) …

Scanning And Transmission Electron Microscopy Of The Oocyst Wall Of Isospora Lacazei, Clarence A. Speer, Alan A. Marchiondo, Bodo Mueller, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The oocyst wall of Isospora lacazei from sparrows was studied with scanning (SEM) and transmission (TEM) electron microscopy. In TEM, t he oocyst wall consisted of four distinct layers (Ll-4). The innermost layer, Ll, was moderately electron-lucent and 240-285 nm thick; L2 was electronJense and 210-240 nm thick; L3 was moderately electron-lucent and 15-150 nm thick; L4, the outer most layer, was discontinuous and consisted of electron-dense discoid bodies which measured 180-220 nm x 320-840 nm. The discoid bodies of L4 as seen by TEM appeared spheroid in shape when observed by SEM. One or two membranes were situated on …

Endoparasites, John E. Ubelaker, Robert D. Specian, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The leaf-nosed bats of the New World family Phyllostomatidae occur from the southwestern United States through tropical Central and South America. Members of this family are also found throughout the Antilles. The ecological associations of the species in this family seem to be rather broad; species are found in humid tropical to semiarid and arid subtropical environments. Diversity in feeding is readily apparent ranging from nectivores (Glossophaga), frugivores (Artibeus), sanguivores (Desmodus), to omnivores (Phyllostomus) (see review by Glass, 1970; Gardner, this volume).

To understand better the biology of phyllostomatid bats, it is …

Fertilizing Capacity And Ultrastructure Of Fowl And Turkey Spermatozoa Before And After Freezing, M. R. Bakst, T. J. Sexton

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The fertilizing capacity, motility and ultrastructure of fowl and turkey spermatozoa were examined at various stages of the freezing process. For both species, fertility and motility were depressed after equilibration with dimethylsulphoxide at 5 °C. After freezing, motility was maintained at 55% for fowl spermatozoa and 40% for turkey spermatozoa; however, fertility was 55% for the fowl and 0% for the turkey. Qualitatively, the damage to the spermatozoa of both species was nearly identical, as revealed by scanning and transmission electron microscopy. The plasmalemma was the primary site of damage. 'Bent' spermatozoa, coiled tails and swollen mitochondria were also present. …

Siderocapsa Major-- Fact Or Artifact?, Ray W. Swanson

Masters Theses

The validity of the genus Siderocapsa, a group of unicellular iron-depositing bacteria, has been a subject of debate for many years. Lack of photomicroscopic and cultural studies has kept this group in taxonomic obscurity. Bacteria were collected from the Campus pond, Eastern Illinois University, Charleston, Illinois, by a submerged slide/coverslip technique. These bacteria were identified as Siderocapsa major according to the description of Molisch (1909). Scanning electron microscopy of Siderocapsa major microcolonies has shown cells (0.5 by 1.15 μm), in a clear central well (2-6 μm), surrounded by an amorphous material which accumulates with the age of the colony. …

Biosystematic Studies Of Impatiens Pallida And Impatiens Biflora (Balsaminaceae), Sandra M. Buening

Masters Theses

Impatiens pallida Nutt. and Impatiens biflora Walt. are two closely related species which grow together in mixed populations but do not hybridize. Flower buds of each species were enclosed with glycine bags to prevent insect pollination. These were left isolated until after anthesis to test for self-pollination and self-compatibility. Neither capsule formation nor seed set were observed.

Chromosomal smears showed that each species has a haploid number of ten chromosomes. Polyploidy was not observed.

Field observations of insect pollinators (primarily bees) showed indiscriminate visitation.

Flower buds were again bagged as before. During anthesis, individuals of the two species were cross-pollinated …

Bacteriophage Populations In Wastewater Effluent, Guy William Lawrence

Masters Theses

Effluent samples were taken at various stages of sewage treatment at a local wastewater treatment plant. Primary, secondary, tertiary and post-chlorinated effluents were sampled. The agar overlay method was used to determine the numbers of plaque-forming bacteriophage particles on thirty-six species of enteric bacteria from the Eastern Illinois University stock culture collection. Twenty-three pure culture phage isolates were prepared from selected plaques and their host range specificity was determined. For every host organism tested, a fifty percent or greater reduction of bacteriophage was evident through the sewage treatment process. Results obtained from investigation of host range specificity indicate that potential …

Studies On The Activation And Inhibition Of Erythrocytic Delta-Aminolevulinic Acid Dehydratase (Alad), Michael James Avram

Dissertations

No abstract provided.

Actions Of L-Methyl-3-Isobutyl Xanthine On The Cat Neuromuscular Junction, Alan C. Roman

Dissertations

No abstract provided.