Digital Commons Network^™

Differences Associated With Mating Type Alleles In Myxomycetes, Robert M. Queen

Retrospective Theses and Dissertations

Differences associated with the mating type alleles of Didymium iridis and Physarum polycephalum were examined with fluorescent antibody and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Heteroabsorption of each anti-myxamoebal serum followed by testing serum activity using immunofluorescence showed there is no strain-specific activity in any of the anti-myxamoebal sera, but the sera was shown, to be genera specific. Intergeneric differences and similarities were shown in the electrophoretic patterns of the myxamoebal protein extracts from P. polycephalum, D. iridis, and Dictyostelium discoideum when compared. Intraspecific differences were noted in D. iridis.

Louisiana Agriculture Magazine, Spring 1982, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

A Laboratory Study Of Nongenetic Embryonic Adaptation To Salinity And Its Subsequent Effects Upon Larval Development Of The Grass Shrimp Palaemonetes Pugio Holthius, Paul Jay Anninos

OES Theses and Dissertations

Fertilized embryos of the grass shrimp Palamonetes pugio Holthuis (Decapoda; Caridea) were exposed to two levels of salinity [5 ppt (exposed) and 20 ppt (control)] during incubation to determine the extent to which embryonic adaptation influences subsequent larval development. Larval response to embryonic exposure was measured at three salinities (5 ppt, 10 ppt, and 20 ppt) as the fraction of larvae surviving to metamorphosis and the duration (in days) of larval development. The survival rate of larvae hatched from control, or non-adapted, embryos was significantly influenced by rearing salinity. The differences in survival of pre-adapted larvae (exposed group), however, were …

The Immunology Of Inflammatory Bowel Disease, Hugh L. N. Mackechnie

Henry Ford Hospital Medical Journal

Although several measurements of both cellular and humoral immunity are altered in inflammatory bowel disease (IBD), no direct pathogenic role can be confirmed in either ulcerative colitis (UC) or Crohn's disease (CD). No clear role has been shown for allergic, microbiological, or psychogenic factors, and no specific genetic susceptibility has been demonstrated. Several studies have shown some benefit from using immunosuppressive drugs to treat inflammatory bowel disease, such as sulfasalazine to treat ulcerative colitis and corticosteroids to treat Crohn's disease. Whether these benefits derive from the anti-inflammatory activity of these drugs or from their immunological effects is uncertain.

Partial Reconstruction Of The Microvillus Core Bundle: Characterization Of Villin As A Ca(++)-Dependent, Actin-Bundling/Depolymerizing Protein, Paul T. Matsudaira, David Burgess

Dartmouth Scholarship

The brush border, isolated from chicken intestine epithelial cells, contains the 95,000 relative molecular mass (M(r)) polypeptide, villin. This report describes the purification and characterization of villin as a Ca(++)-dependent, actin bundling/depolymerizing protein. Then 100,000 g supernatant from a Ca(++) extract of isolated brush borders is composed of three polypeptides of 95,000 (villin), 68,000 (fimbrin), and 42,000 M(r) (actin). Villin, following purification from this extract by differential ammonium sulfate precipitation and ion-exchange chromatography, was mixed with skeletal muscle F-actin. Electron microscopy of negatively stained preparations of these villin-actin mixtures showed that filament bundles were present. This viscosity, sedimentability, and ultrastructural …

Uv Light-Induced Survival Response In A Highly Radiation-Resistant Isolate Of The Moraxella-Acinetobacter Group, Lewis Keller, Thomas Thompson, R. Burt Maxcy

Department of Food Science and Technology: Faculty Publications

A highly radiation-resistant member of the Moraxella-Acinetobacter group, isolate 4, obtained from meat, was studied to determine the effect of preexposure to UV radiation on subsequent UV light resistance. Cultures that were preexposed to UV light and incubated for a short time in plate count broth exhibited increased survival of a UV light challenge dose. This response was inhibited in the presence of chloramphenicol. Frequencies of mutation to streptomycin, trimethoprim, and sulfanilamide resistance remained the same after the induction of this survival response and were not altered by treatment with mutagens, with the exception of mutation to streptomycin resistance after …

Fifth Biennial Cheese Industry Conference, Various Authors

Cheese Industry Conference

No abstract provided.

Protein Bodies In Dormant, Imbibed And Germinated Sunflower Cotyledons, R. D. Allen, H. J. Arnott

Food Structure

Scanning electron microscopy was used to observe the morphology and structure of protein bodies in dormant and imbibed sunflower cotyledons and to document the morphological changes in protein bodies during germination and seedling growth. In order to clearly visualize dormant seed structure, anhydrous fixation techniques were employed. Definite differences in cellular structure are seen in comparisons of dry and imbibed seed tissues. As germination proceeds, protein bodies lose their smooth spherical shape and become indented and pitted. Protein body coalescence and fusion precedes the formation of a central protein vacuole. As protein is hydrolyzed, protein vacuole density decreases, and its …

Electron Microscopy Of Milk And Milk Products: Problems And Possibilities, D. G. Schmidt

Food Structure

Milk and dairy products have frequently been studied by transmission- and scanning electron microscopy. The specimen preparation procedure may considerably influence the final result, and formation of artefacts is frequently observed. In this respect, formation of ice crystals during cryofixation is a well-known phenomenon. But dehydration, to an extent such as is required for embedding procedures, also appears to be harmful to dairy products. Micrographs of thin sections of plastic- embedded samples of casein submicelles show threadlike material, whereas in freeze-etched specimens only spherical particles are found. Similar observations are made when samples of cheese and of concentrated milk are …

Fluorescence Microscopy Of Cereals, R. G. Fulcher

Food Structure

The fluorescence microscope is one of the most sensitive instruments available for morphological and microchemical analysis of biological material, and especially of cereal grains. Recent innovations in illuminating systems, fluorescence chemistry, and specimen preparation have combined to provide significant improvements over conventional bright-field microscopy in both specificity and sensitivity. A variety of relatively specific fluorescent markers has been devised for routine and high resolution detection of all major cereal components. Several examples of useful fluorescent markers are described, including appropriate methods for specimen preparation, fluorescence analysis, and photography.

Food Microstructure: An Integrative Approach, E. A. Davis, J. Gordon

Food Structure

The integration of information at all levels of organization in a food system - atomic and molecular through macroscopic properties is illustrated with cake batters as an example of a formulated food and bovine muscle as an example of a biologically intact, non-formulated food. Direct examples are given using macroscopic data collected in heat and water transport studies followed by integration of microscopical data for interpretive purposes.

The contribution of starch gelatinization to the characteristics of water loss in batter is related to events such as loss of brief ringence as seen by polarizing microscopy , differential heat input as …

Aspects Of Sample Preparation For Freeze-Fracture/Freeze-Etch Studies Of Proteins And Lipids In Food Systems. A Review, Wolfgang Buchheim

Food Structure

To select optimum specimen preparation methods and to correctly interpret freeze-fracture/freezeetch micrographs of food systems a detailed knowledge of the individual steps of preparation -- i.e. chemical fixation of samples, their cryoprotective pretreatment, cryofixation, freeze-fracturing and -etching, and replication -- and of their influence on the appearance of different constituents, especially proteins and lipids, is necessary. Food systems show great variation in composition, structure and especially in their content of water -- e.g. molecular and colloidal solutions, oil-in-water and water-in-oil emulsions, gels suspensions, semi-solid systems such as cheese, dried systems such as milk powders -- thus requiring a careful variation …

Experiences In The Protection Of The Large Predators In Finland, Erkki Pulliainen

International Journal for the Study of Animal Problems

During the nineteenth century, the large predators of Finland- wolf, bear, lynx, and wolverine- were exterminated in the southern and western regions of the country. There were almost no lynx by late 1950. However, a protection order issued in 1968 resulted in a steady increase in their number, to about 300 by 1980. A breeding wolverine population existed until the late 1960s. In the 1970s, most were killed by snowmobiles. It is now thought only 10-30 inhabit the frontiers between Finland and the USSR and Finland and Norway. Bears, in the 1970s, tended to immigrate to Finland from the east; …

News And Analysis

International Journal for the Study of Animal Problems

The section includes reports on the use of pound animals for research; EEC standards for battery cages for laying hens; a declaration that fish are considered to be animals in Massachusetts, the rescue of dogs from research in Maryland; determining wildlife populations in Virginia; the tuna-dolphin controversy; the protection of laboratory animals; animal experimentation in the UK; the AVMA Animal Welfare Committee; broiler chicken welfare; ignorance regarding wildlife in the USA, the Ames Test as an alternative; the ban on sperm whale hunting; and an analysis of alternatives research supported by the NIH.

Deep Woodchip Litter: Hygiene, Feeding, And Behavioral Enhancement In Eight Primate Species, Arnold S. Chamove, James R. Anderson, Susan C. Morgan-Jones, Susan P. Jones

International Journal for the Study of Animal Problems

Sixty-seven animals from eight primate species were used to assess improved husbandry techniques. The presence of woodchips as a direct-contact litter decreased inactivity and fighting and increased time spent on the ground. Placing food in the deep litter led to further behavioral improvement. Frozen foods improved distribution and reduced fighting in most situations, especially when buried in the litter. With time, the litter became increasingly inhibitory to bacteria. The results suggest that inexpensive ways of increasing environmental complexity are effective in improving housing for primates.

Experiences In The Protection Of The Large Predators In Finland, Erkki Pulliainen

Ecology Collection

During the nineteenth century, the large predators of Finland- wolf, bear, lynx, and wolverine- were exterminated in the southern and western regions of the country. There were almost no lynx by the late 1950s, but a protection order issued in 1968 has resulted in a steady increase in their number, to about 300 by 1980. There was a breeding population of wolverines until the late 1960s, but in the 1970s, most were killed by snowmobiles, and only 10-30 are now thought to inhabit the frontiers between Finland and the USSR, and Finland and Norway. Bears, in the 1970s, tended to …

Deep Woodchip Litter: Hygiene, Feeding, And Behavioral Enhancement In Eight Primate Species, Arnold S. Chamove, James R. Anderson, Susan C. Morgan-Jones, Susan P. Jones

Ethology Collection

Sixty-seven animals from eight primate species were used to assess improved husbandry techniques. The presence of woodchips as a direct-contact litter decreased inactivity and fighting, and increased time spent on the ground. Placing food in the deep litter led to further behavioral improvement. The use of frozen foods improved food distribution and reduced fighting in most situations, especially when it was buried in the litter. With time, the litter became increasingly inhibitory to bacteria. The results suggest that inexpensive ways of increasing environmental complexity are effective in improving housing for primates.

G82-622 Sources Of Grain Market Information, Allen C. Wellman

University of Nebraska-Lincoln Extension: Historical Materials

A list of various market information sources, each followed by a brief summary of distribution schedules and contents.

This NebGuide lists widely used and readily available market information sources that contain information which may be useful to agricultural producers, lenders and agribusiness firms when making grain marketing decisions. Most of the available market information and statistical data comes from the U.S. Department of Agriculture (USDA).

Generally, there are two basic types of market information available to data users. The first type, Market News Service, is provided by the USDA's Agricultural Marketing Service (AMS) and concerns daily prices used to make …

The Microscopic Structure And Chemistry Of Rapeseed And Its Products, S. H. Yiu, H. Poon, R. G. Fulcher, I. Altosaar

Food Structure

The location and distribution of some of the storage constituents in the structures of rapeseed and its products were investigated. Hand-cut or glycol methacrylate-embedded sections were stained with dyes or fluorochromes of known specificities and examined using fluorescence, bright-field and/or polarizing microscopy. Results obtained from the study were based upon observations of characteristics of the various stainings, birefringence, induced fluorescence and autofluorescence. The effects of enzymatic hydrolysis, solvents and processing on the cellular structures and their affinity for certain dyes/ fluorochromes were also investigated. Major and minor storage constituents were tentatively located in the structure of rapeseed. Lipids and proteins …

Freeze-Etch Of Emulsified Cake Batters During Baking, J. D. Cloke, J. Gordon, E. A. Davis

Food Structure

Cryofixation, freeze-etch techniques were used to study the structure of cake batters made from a lean cake formulation before heating and after heating to temperatures up to l00-l02°C. Batters were prepared without added emulsifiers and with saturated and unsaturated monoglycerides replacing 5 and l 0% of the oil. Unsaturated monoglyceri des were more effective than saturated monoglycerides in dispersing oil droplets through the batter. Saturated monoglycerides formed liquid crystals during baking. The temperature at which starch granules began to swell was slightly higher for saturated monoglyceride containing cakes. The batter matrix between starch granules was more clearly defined in unsaturated …

Light Microscopy Preparation Techniques For Starch And Lipid Containing Snack Foods, F. Olga Flint

Food Structure

Many processed foods lack the structural integrity associated with biological tissue so that the conventional methods of preparation and staining used in light microscopy may introduce misleading artifacts.

Taking as examples of starch-based processed foods, potato chips (UK potato crisps) and three distinct potato snack foods, methods for preparing and demonstrating the constituents present in cryosections of whole and masticated products are discussed. To show constituents in their true relative locations vapor staining and polarized light are used. Iodine vapor staining indicates the extent of starch gelatinisation in the dry snack and it is also used to show the structural …

Some Effects Of Lipids On The Structure Of Foods, K. Larsson

Food Structure

The functional properties of different lipids in foods are demonstrated and related to the structure of lipid or lipid-water phases. On the basis of new X-ray data on the crystal structure of the B'-form and the a + B' transition in fats, the polymorphic transitions are considered as different lateral arrangements of triglyceride dimers. The physical properties of fat crystals can be explained from the structures, as well as possibilities to influence the polymorphic transitions.

Molecular interaction between polar lipids and proteins or starch is discussed, and the effect of the amylose-lipid inclusion complex on gelatinization temperature and water penetration …

The Uptake And Release Of Label From Endocytosed 35so4-Labeled Proteins In Cultured Mouse Peritoneal Macrophages, Susan Patterson Buktenica

Dissertations

No abstract provided.

Bk Virus Transformation Of Bhk-21 Cells, Martha A. Lampert

Dissertations

No abstract provided.

G82-621 Sources Of Livestock And Poultry Market Information (Revised July 1994), Allen Wellman

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide provides a list of various market information sources, each followed by a brief summary of issue schedules and contents.

It provides a listing of widely used and readily available market information sources that contain information which may be useful to agricultural producers, lenders and agribusiness firms when making livestock and poultry marketing decisions. Most of the available market information and statistical data comes from the U.S. Department of Agriculture (USDA). Many now require an annual subscription fee.

Dark Repair Of Lethal Damage Induced In A Hybrid Mammalian Tissue Culture Cell Line By Ultraviolet Light, Robin Haetten, Margaret Mcguinness, H. Gaston Griggs

Journal of the Arkansas Academy of Science

No abstract provided.

Enflagellation Of Naegleria Fowleri, Terry Wayne Woodworth

Theses and Dissertations

Organisms of the genus Naegleria differentiate from feeding, dividing amebae into temporary swimming flagellates when deprived of nutrients. Factors critical for enflagellation of the pathogenic species, N. fowleri, were delineated and optimal conditions established for evoking reproducible conversion of N.fowleri populations. N. fowleri enflagellation differed from that of N. gruberi in its dependence upon growth phase, population density during enflagellation and in the timing and extent of the conversion. N. fowleri amebae from stationary phase cultures grown at 37°C, washed free of medium and suspended in nutrient~free ameba saline, acquired flagella and converted to mature flagellates in a synchronous manner …

Effects Of Pulsed Electric Fields On Mammalian Cell Membranes, Guy K. Smith

Theses and Dissertations

The effect of pulsed electric fields on cell membranes were studied to investigate the effects of an electric field, per se, on biological systems, without the densometric and other technical problems associated with other forms of nonionizing radiation. Exposure of mouse splenocytes to a high voltage pulse resulted in an increase in membrane permeability to K⁺ that was dependent on both the electric field strength and the pulse duration. Exposure to a 2 μsec, 3.0 kV/cm pulse elicited a 50% loss of intracellular K⁺ indicating that the critical transmembrane potential (V_m) at breakdown was 1.26 volts …

Louisiana Agriculture Magazine, Winter 1982-1983, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Effect Of Container Size And Harvest Date On The Growth Of Quaking Aspen Populus-Tremuloides Seedlings, L. A. Erhard, J. T. Fisher

Aspen Bibliography

Quaking Aspen (Populus tremuloides Michaux) were
greenhouse grown in four sizes of paraffin-coated containers: 185 cubic centimeters (11 cubic inches), 492 cubic centimeters (30 cubic inches), 1132 cubic centimeters (68 cubic inches), and 1472 cubic centimeters (90cubic inches).