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Interpretation Of Structural Patterns Appearing On Corrosion Casts Of Small Blood And Initial Lymphatic Vessels, A. Castenholz

Scanning Microscopy

Structures imprinted on the corrosion casts of blood and lymphatic microvessels are examined by correlative scanning electron microscopy of corroded casts and fixed tissue. Replicas of the endothelial nuclear portions and cell boundaries and the plastic bands surrounding the casts of terminal blood vessels are demonstrated. In small lymphatics the replication of valve structures, interendothelial open junctions and direct prelymphatic connections is shown. The different imprint patterns are examined in regard to their morphological significance and under methodical aspects.

Effect Of Milk On Fibronectin And Collagen Type I Binding To Staphylococcus Aureus And Coagulase-Negative Staphylococci Isolated From Bovine Mastitis, J. Miedzobrodzki, A. S. Naidu, J. L. Watts, Pawel Ciborowski, K. Palm

Nebraska Center for Virology: Faculty Publications

Tryptic soy broth (TSB)-grown cells of Staphylococcus aureus isolated from acute and chronic bovine mastitis bound mainly ¹²⁵I-fibronectin (¹²⁵I-Fn), whereas strains of nine species of coagulase-negative staphylococci showed a predominant interaction with ¹²⁵I-collagen (¹²⁵I-Cn) type I. A particle agglutination assay (PAA) was used to examine the interaction of coagulase-negative staphylococci with ¹²⁵1-Fn and ¹²⁵I-Cn immobilized on latex. All 368 coagulase-negative staphylococci demonstrated high ¹²⁵I-Cn and moderate to low ¹²⁵I-Fn interactions in the PAA. Cn-PAA reactivity was high among strains of Staphylococcus xylosus (84.2%), Staphylococcus simulans (77.8%), Staphylococcus epidermidis (76.7%), and …

Advances In The Diagnosis Of Urothelial Neoplasia, John D. Crissman, Richard J. Zarbo, Terri Johnson, V. Ramesh Babu, Fazlul H. Sarkar, Brian J. Miles, Joseph C. Cerny

Henry Ford Hospital Medical Journal

Urothelial neoplasia is a unique cancer in that it consists of a spectrum of tumors with different biologic behaviors. The most common urothelial neoplasm is the low grade superficial papillary carcinoma or papilloma which may recur numerous times but does not result in significant morbidity or mortality. A variant of the superficial papillary carcinoma, which represents approximately 10% of the tumors, is the noninvasive papillary neoplasm which progresses to a less differentiated invasive transitional cell carcinoma (TCC). Considerable effort has been directed at identifying which of the superficial well differentiated papillary tumors will persist, recur and progress to invasive cancer …

Bradyrhizobium Japonicum Survival In And Soybean Inoculation With Fluid Gels, Michael D. Jawson, Alan J. Franzluebbers, Robert K. Berg

Department of Agronomy and Horticulture: Faculty Publications

The utilization of gels, which are used for fluid drilling of seeds, as carriers of Bradyrhizobium japonicum for soybean (Glycine max (L.) Merr.) inoculation was studied. Gels of various chemical composition (magnesium silicate, potassium acrylate-acrylamide, grafted starch, and hydroxyethyl cellulose) were used, although the hydroxyethyl cellulose gels were more extensively investigated. Gel inocula were prepared by mixing gel powder with liquid cultures of B. japonicum (2% [wt/vol]). The population of B. japonicum USDA 110 did not change in each gel type during 8 days of incubation at 28°C. These fluid gels were prepared with late-exponential-growth-phase cells that were washed …

Architecture Of The Medial Smooth Muscle Of The Arterial Vessels In The Normal Human Brain: A Scanning Electron-Microscopic Study, Toshitaka Shiraishi, Saburo Sakaki, Yasuo Uehara

Scanning Microscopy

The architecture of the medial smooth muscle of arterial vessels in normal human brains was investigated using scanning electron microscopy. We could divide the arterial vessels into four subdivisions according to the number of the circular muscle cells. The arteries ( > 100 μm in diameter) had 4-20 layers of circular smooth muscle cells; individual circular muscle cells were spindle-shaped and occasionally had branches at their ends. Multidirectional muscle cells were observed in the medio-adventitial border only at the branching sites in large arteries (>300 μm), but at both branching and non-branching sites in the small arteries (100-300 μm). The …

X-Ray Microanalysis Of Calcium Containing Organelles In Resin Embedded Tissue, G. Nicaise, I. Gillot, A. K. Julliard, E. Keicher, S. Blaineau, J. Amsellem, J. C. Meyran, M. L. Hernandez-Nicaise, B. Ciapa, C. Gleyzal

Scanning Microscopy

The localization of calcium in cell organelles at the electron microscope level is often achieved through cytochemical techniques, and verified by X-ray microanalysis. Various methods have been used to cytochemically detect calcium or calcium-binding sites : calcium loading, calcium substitution by strontium, barium, or even lead, and calcium precipitation by oxalate, phosphate, fluoride, or pyroantimonate. Their results may have heuristic value, particularly in preliminary studies of poorly known cell types. A complementary and more physiological approach is offered by quantitative measurement of the total calcium content of organelles after cryofixation.

Resin embedding is less demanding than cryomicrotomy and gives better …

Feeding Practices In South Dakota Cattle Feedlots, D C. Taylor, J. J. Wagner, R. D. Kappes

South Dakota Beef Report, 1989

During March 1989, a mail survey of South Dakota cattle feedlot managers was undertaken. The purposes of the survey were to characterize the nature of the cattle feeding industry in South Dakota and to determine the relationships between (i) each of size-of-feedlot and geographic location within the state and (ii) management practices followed by cattle feeders. Direct relationships exist between size-of-feedlot and the following: (1) rate of feedlot utilization in each quarter of the year (P<.10); (2) percentage grain relative to roughage in both growing and finishing diets (P<.10); (3) percentage of feedlots feeding high moisture grain, cracked grain, and ground hay (P<.01); (4) percentage of feedlots using rumen stimulants and growth implants (P<.01); and (5) percentages of managers testing feeds for nutrient composition, using feed scales to control feeding rates, maintaining feed records for separate pens of cattle, and hiring consultants to formulate rations (P<.01). On the other hand, inverse relationships exist between size-of-feedlot and the following: (6) days on feed for heifer calves, yearling steers, and yearling heifers (P<.10); (7) slaughter weight of steers (Pc.10); (8) percentage of home-raised hay and dry grain (P<.10); (9) percentage of feedlots feeding ground grain and unprocessed hay (P<.01); and (10) percentage of feedlots not using feed additives (P<.10). Average days on feed for steer and heifer calves are lower (P<.05) in the West than in other areas of the state. More milo is fed in the West; more barley is fed in the North Central region; and less home-raised corn silage and haylage are fed in the West than in other regions (P<.05).

Quantitative Microanalysis Of Nitrogen Distribution In Carpospores Of Gracilaria Secundata With Scanning Auger Microscopy, Åke Lignell, Rein Kalm, Godfried M. Roomans, Angelica Von Hofsten, Olof Vingsbo

Scanning Microscopy

Quantitative microanalysis has been performed with a JAMP 10 Scanning Auger Microscope at 0.5 μm spot size, on carpospores of Gracilaria secundata Harv.. Samples were fixed in glutaraldehyde and osmium tetroxide and embedded in plastic material. Thin sections were placed on solid copper discs. the surfaces of which had been smoothed by electropolishing.

Quantification of the nitrogen content was performed using a standard with known content of nitrogen.

Nitrogen was localized in the nucleus, chloroplasts and osmiophilic inclusions of the carpospore. Trace amounts of nitrogen were also found in cell walls, starch grains and in the embedding material.

The use …

Surface Studies Of Calcium Oxalate Dihydrate Single Crystals During Dissolution In The Presence Of Stone-Formers' Urine, M. Akbarieh, R. Tawashi

Scanning Microscopy

Dissolution of calcium oxalate dihydrate (COD) single crystals was studied at different pH levels and in different dilutions of stone formers' (SF) urine. The Fourier descriptors of the contour were determined during dissolution of COD using a quantitative morphological technique. The surface ruggedness of COD single crystals was determined from fractal analysis. The results obtained were compared with COD single crystals behavior in different dilutions of normal urine previously reported. The shape parameters and surface geometry of the dissolving COD crystals were significantly different in normal and SF urine. The data suggest that the shape descriptors and fractal geometry is …

Synergistic Antifungal Action Of 8-Quinoinol And Its Bischelate With Copper(Ii) And Mixed Ligand Chelates Composed Of Coper(Ii), 8-Quinolinol, And Aromatic Hydroxy Acids / Herman Gershon, Donald D., Clarke, And Muriel Gershon, Herman Gershon, Donald Dudley Clarke Phd, Muriel Gershon

Chemistry Faculty Publications

Antifungal studies were made of mixtures of minimal inhibitory concentrations (MICs) of 8-quinolinol and its bischelates with copper(II), zinc(II), and manganese(II) and with mixed ligand chelates composed of 8-quinolinol, copper(II) and a second ligand including salicylic acid, 3-hydroxy-2-naphthoic acid, 3,5-diiodosalicylic acid, and 4-bromo-3-hydroxy-2-naphthoic acid. Mixtures of the MICs of the bischelates of 8-quinolinol with copper(II) and zinc(II) and copper(II) and manganese(II), as well as 7-iodo-8-quinolinol and its bischelate with copper(II), and 8-quinolinol and 5-iodo-8-quinolinol were also studied against six fungi: Aspergillus niger, Aspergillus oryzae, Trichoderma viride, Myrothecium verrucaria, Mucor cirinelloides, and Trichophyton mentagrophytes. With the exceptions of the mixtures of …

Program Abstracts, 101st Session, Iowa Academy Of Science, April 21-22, 1989, Buena Vista College

Journal of the Iowa Academy of Science: JIAS

No abstract provided.

Santa Clara Magazine, Volume 31 Number 2, Winter 1989, Santa Clara University

Santa Clara Magazine

10 - MANUFACTURED MOTHERHOOD A Catholic theologian looks at reproductive technology.

16 - ELECTION '88 Will Bush put a new face on Uncle Sam?

20 - IN THE FACE OF AIDS What alumna Sharon Kugler '81 learned hel ping AIDS sufferers.

23 - ON A ROLL New York's hottest director is SCU's Ron Lagomarsino '73.

25 - DO JOBS AND COLLEGE MIX? Alumni and students tell how they fit part-time jobs into their schedules/

Furniture Manufacturing And Marketing In The ‘American Economic Transition, Steven H. Bullard

Faculty Publications

The Office of Technology Assessment recently prepared a 500 page report titled Technology and the American Economic Transition, Choices for the Future. OTA sees four major factors that are resulting in "economic transition" factors that will continue to transform the U.S. economy during the next 20 years: new technologies, new challenges from abroad, new resource constraints, and new values and tastes in U.S. consumer and labor markets.

New technologies, particularly in collecting and handling information, are expected to have long-term, pervasive economic and social impacts comparable to past U.S. economic transformations from major technologies such as automobiles and railroads. New …

Death, Suffering, Predation, Animal Rights And Interpretation, Denis Hahn, Paul Risk

Faculty Publications

No abstract provided.

The U.S. Household Furniture Industry: Adapting To Changing Times., Brian A. Doherty, Steven H. Bullard

Faculty Publications

The U.S. wood household furniture industry has adapted to change in many ways in recent years. The industry has grown steadily since the 1982 recession, and has been shifting to the U.S . South and Southeast. Although California has also become prominent in furniture production, many recent factors indicate geographic shifts away from the state, particularly in wood furniture production. The household furniture industry has also adapted and continues to adapt to factors which have increased the globalization of trade in all products. Foreign competition has resulted in mergers and consolidation in the U.S. household furniture industry, and has also …

Desert Explorations - A Videodisc Exhibit Designed For Flexibility, Judy Diamond, Alan B. Bond, Atsusi Hirumi

Alan Bond Publications

Using an interactive exhibit does not provide the same experience for everyone. How visitors understand an exhibit, what associations it evokes, and even how it is physically utilized may differ widely. This article describes an exhibit that was designed to let visitors tailor their explorations to their own interests and motivation. The exhibit, called Desert Explorations, gives visitors choices of what to see and how to learn about what they see. This exhibit also is an example of a way of developing interactive videodisc exhibits that is relatively inexpensive yet may be tailored to a particular topic or hall. This …

On The Repair Of Dna Breaks And The Specificity Of The Ecori Restriction Enzyme, Joseph Heitman

Student Theses and Dissertations

We would like to understand how proteins and enzymes interact with DNA. We describe here our studies of the Mrr methylation dependent restriction system, DNA single- and double-strand break repair in E. coli, and substrate recognition by the EcoRI endonuclease. Many species of bacteria make restriction-modification systems to destroy foreign DNA that enters the cell. These systems usually consist of an endonuclease that cleaves a specific DNA sequence and a methylase which modifies the DNA to protect the host chromosome. We observed that when foreign site-specific methylases are expressed in E. coli, the SOS DNA repair response is induced. This …

Molecular Cloning Of Avian Sarcoma Virus Ct10 And Characterization Of Its Protein Product, Bruce J. Mayer

Student Theses and Dissertations

Four previously uncharacterized avian sarcoma viruses were screened and two of these, RPL30 and CT10, were found to encode apparently novel oncogenes. Biologically active CT10 DNA was molecularly cloned and the nucleotide sequence was determined. The CT10 genome encodes a gag-fusion polypeptide of 47 kilodaltons, termed p47^gag-crk. This protein contains blocks of sequence similarity to a noncatalytic, potentially regulatory region found in the nonreceptor tyrosine kinases, a phosphoinositide-specific phospholipase C, and the ras GTPase activator protein; no homology was found to any known catalytic domain. Potential roles for the homologous domains, termed SH2 and SH3, in normal signal …

Dna-Protein Interactions In F1 Dna Replication, David I. Greenstein

Student Theses and Dissertations

This thesis analyzes the interaction of two DNA-binding proteins with the plus strand replication origin of bacteriophage f1. The origin has a bipartite structure consisting of a required core origin region and an adjacent A+T- rich enhancer sequence that potentiates replication approximately 100-fold. The core origin binds the initiator protein, and the enhancer contains three binding sites for the E. coli integration host factor (IHF). Both activator proteins bend the DNA sequence to which they bind, implying that together they wrap the origin DNA into a higher order structure that is active in initiation. The replication initiator protein of bacteriophage …

Studies On The Activity Of The Long Terminal Repeat Of Rous Sarcoma Virus In Animal Cells And In Yeast, Anindya Dutta

Student Theses and Dissertations

Transcription from the Rous sarcoma virus (RSV) Long terminal repeat (LTR) in untransformed rat 3Y1 fibroblasts is dependent on the presence of serum. Within an hour of addition of serum to a serum-deprived culture there is a 5 fold stimulation in the level of transcripts initiated at the LTR. This stimulation does not require synthesis of new proteins. Mutations in the RSV LTR revealed that serum-stimulated transcription was mostly dependent on two CCAAT boxes in the LTR, though other upstream sequences may play a secondary role. Serum caused the rapid appearance of a nuclear protein that binds to the two …

Vacuole Formation In Wheat Starchy Endosperm, D. B. Bechtel, A. Frend, L. A. Kaleikau, J. D. Wilson, P. R. Shewry

Food Structure

The formation of vacuoles in wheat (Triticum aestivum cv. Highbury) starchy endosperm cells was studied using electron microscopy. Some vacuoles were always present, even in the coenocytic cytoplasm. The first formed endosperm cells were highly vacuolated, but became filled with cytoplasm as they grew older. Various-sized pieces of cytoplasm were found in vacuoles of developing endosperm cells, probably as a result of autophagic sequestration. The membranes of the autographic vacuoles appeared to originate from the rough endoplasmic reticulum and from extensions of already-formed vacuoles. Autographic activity was confirmed by localizing the hydrolytic enzyme acid phosphatase within the vacuoles. The rough …

An Electron Microscopic Study Of The Adherence Of Lactobacillus Acidophilus To Human Intestinal Cells In Vitro, S. K. Hood, E. A. Zottola

Food Structure

In this study, transmission electron microscopy was used to visualize the adherence of Lactobacillus acidophilus to human intestinal tissue cells (HITC) in vitro. There appeared to be a layer of electron dense material on the bacterial cell and on the intestinal cell which may mediate adherence. When L. acidophilus attached to intestinal tissue cells after a short contact period, it did not appear to disrupt the integrity of the intestinal cell. Treatment of the bacterium with sodium periodate and concanavalin A reduced the adherence to HITC, suggesting that a carbohydrate was involved. Electron micrographs of periodate-treated cells revealed that the …

Trichome Development In Arabidopsis Thaliana. 1. T-Dna Tagging Of The Glabrous1 Gene, M. David Marks, Kenneth A. Feldmann

School of Biological Sciences: Faculty Publications

Progeny from a transformed Arabidopsis plant (produced by the Agrobacterium-mediated seed transformation procedure) were found to be segregating for an altered trichome phenotype. The mutant plants have normal leaf trichomes but completely lack trichomes usually found on the stem. The mutation is tightly linked to a T-DNA insert. Complementation analysis with genetically characterized trichome mutants revealed that the new mutation is an allele of the GL1 locus. The new trichome mutant has been designated gl1-43. DNA gel blot analysis indicated that the insert site contains a complex array of at least four tandemly linked T-DNA units oriented as both …

Role Of Metabolites In The Reversible Light Activation Of Pyruvate,Orthophosphate Dikinase In Zea Mays Mesophyll Cells In Vivo, Chrissi A. Roeske, Raymond Chollet

Department of Biochemistry: Faculty Publications

Whole leaf and mesophyll cell concentrations of pyruvate, phosphoenolpyruvate (PEP), ATP, and ADP were determined in Zea mays during the reversible light activation of pyruvate, orthophosphate dikinase in vivo. Mesophyll cell levels of the four metabolites were estimated by extrapolation from values in freeze-quenched leaf samples that were fractionated by differential filtration through nylon mesh nets (adapted from M Stitt, HW Heldt [1985] Planta 164: 179-188). During the 3 minutes required for complete light activation of dikinase, pyruvate levels in the mesophyll cell decreased (from 166 ± 15 to 64 ± 10 nanomoles per milligram of chlorophyll [nmol/mg Chi]) …

Anticholesteremic Property Of Lactobacillus Acidophilus Yogurt Fed To Mature Boars, A. D. Danielson, E. R. Peo, Jr., K. M. Shahani, A. J. Lewis, P. J. Whalen, M. A. Amer, Win Butler

Department of Animal Science: Faculty Publications

Three strains of Lactobacilus acidophilus (LA) were isolated from the feces of mature boars that were not being fed antibiotics from the Nebraska Gene Pool (NGP). All three LA isolates were screened in vitro for anticholesteremic and antimicrobial activities. One strain, LA16, caused the greatest reduction in cholesterol and inhibited both Bacillus subtilis and Escherichia coli the most. ♥

Trichome Development In Arabidopsis Thaliana. Ii. Lsolation And Complementation Of The Glabrous1 Gene, Patricia L. Herman, M. David Marks

School of Biological Sciences: Faculty Publications

We are using the formation of trichomes in Arabidopsis thaliana as a model system to study gene expression during cellular differentiation. To initiate the molecular characterization of this system, we tagged and isolated a gene that is specifically required for the development of the specialized trichome cell. We confirmed the identity of this gene, GLABROUS1 (GL1), by complementation. These results demonstrate that a crucial gene in a plant developmental pathway can be successfully identified by complementation.

Public Policy On The Introduction Of Genetically Engineered Microorganisms, Anne K. Vidaver

Journal of the Iowa Academy of Science: JIAS

This presentation raises questions of research needs and issues. Underlying assumptions are that only beneficial or useful microorganisms will be "released"; that extensive laboratory and contained experiments will have been done prior to introduction and live microorganisms can be confined within the areas of introduction. Evidence to support these assertions will be presented. Critical needs for progress in this area include: 1) Recognition that the nature of the product introduced into the environment is of primary significance, not how the organism was genetically altered or modified. 2) Recognition that microorganisms are introduced into the environment as part of our daily …

Tissue Culture In Rice (Oryza Sativa L. Subsp. Japonica Var. Lemont), Young M. Woo

Masters Theses

The purpose of this study was to obtain callus induction and subsequent plantlet regeneration from various explants of rice (Oryza sativa L. subsp. japonica var. Lemont). Callus induction occurred from the somatic cells of ovary wall, immature and mature embryos, immature endosperm, stem nodes, and seedlings. Plantlets were regenerated from embryo-, endosperm-, and stem node-derived calli on differentiation media. Stem nodes were the most responsive to callus induction. Requirements for optimal callus formation and plantlet regeneration differed from each culture. The study investigated the effects of cold treatment of explants, initial culture in darkness, media, and subculture for individual explant …

Louisiana Agriculture Magazine, Winter 1989-1990, Lsu Agricultural Experiment Station

Louisiana Agriculture

No abstract provided.

Isolation And Immunological Characterization Of A Group Of New B Lymphomas From Cba Mice, Venkatachalam Udhayakumar, Peter H. Brodeur, M. S. Rajagopalan, Stephen Zimmer, Karen E. Pollok, Subbarao Bondada

Microbiology, Immunology, and Molecular Genetics Faculty Publications

We have isolated and characterized a new series of B lymphomas which occurred spontaneously in a group of CBA N mice that were transferred with spleen or lymph node cells from 24-month-old CBA Ca mice. Tumor cell lines from six CBA N mice that received spleen cells were rescued and designated as BKS-2, BKS-3, BKS-4, BKS-5, BKS-6, and BKS-7. Also, tumor cells from a recipient of lymph node cells were rescued and the resulting cell line was designated BKL. These tumor cells expressed membrane immunoglobulin (μ,κ), major histocompatibility complex Class I and Class II molecules, B220, Lyb8, Fc receptors, J11d, …