Digital Commons Network^™

Traction Force Microscopy Using An Epifluorescence Microscope: Experimental Considerations And Caveats, Zaria Booth, Mazen Mezher, Rudra Patel, Venkat Maruthamuthu

Mechanical & Aerospace Engineering Faculty Publications

Forces exerted by cells due to their internal contractility play fundamental roles in a host of processes, including adhesion, migration, survival and differentiation. Traction force microscopy (TFM) enables the determination of forces exerted by cells or cell collectives on their environment, which is typically taken to be an extra-cellular matrix (ECM)-coated substrate. Sample preparation for TFM involves the plating of cells onto an environment embedded with fiducial markers. The imaging of these fiducial markers in the presence and absence of the cells then enables calculation of the displacement of localized regions of the environment, and, consequently, the spatial distribution of …

Assessing The Role Of Daily Thermal Fluctuations In The Performance Of Mitochondrial Energy Transduction Of The Centrarchid, Lepomis Cyanellus, Grayson Hofmann

Masters Theses

Freshwater systems are affected by current climate extremes, forcing species found in these systems to withstand drastic changes in temperature. Temperature changes affect these organisms by altering their metabolic rate and energy balance. Increases in temperature can lead to an increase in oxygen consumption, which ultimately increases the need for more energy production. Mitochondrial energy transduction of green sunfish (Lepomis cyanellus) and many other fish species after a thermal insult, has hardly been explored. This study complements the limited research available on mitochondrial function in similar species, as they ignore the possibility of consecutive thermal events, which are …

Habitat Use And Selection Of Silver Carp In The Lower Wabash River System, Coy B. Blair

Masters Theses

The spatial ecology of non-native species in invaded systems remains relatively poorly understood despite the importance of suitable habitat in the success of invasion. Silver Carp Hypophthalmichthys molitrix are a highly invasive non-native species with established populations throughout the Midwestern United States. Much research has focused on habitat use of bigheaded carp in large river systems, though invasion risk remains high in smaller systems, such as tributaries of the Great Lakes and the Ohio River. The Wabash River, a unique system in the Midwest, remains largely un-impounded and is comparable to many of the smaller riverine systems at risk of …

Examining Group Components On Exercise Adherence In Older Adults, Brody Polk

Masters Theses

Older adults remain the least active population despite the well-known benefits of regular exercise (Beauchamp et al., 2007; Rúa-Alonso et al., 2023). Researchers examining exercise adherence in older adults have determined two modalities of training—individual and group—with individual training being the preferred mode of exercise for older adults (Beauchamp et al., 2007). However, previous research has failed to evaluate the various components of group exercise before making a comparison with individual exercise to determine which form best predicts older adults’ adherence to exercise (Hawley-Hague et al., 2014). As such, the purpose of the current study was to examine the effects …

Functional Characterization Of The Hdt5 Gene In Aspergillus Flavus Morphogenesis, Mycotoxin Production, And Fungal Phytopathogenicity, Brenda Omburo

Graduate Research Theses & Dissertations

The present study aimed at elucidating the role of hdt5 in fungal morphology, mycotoxin production and in plant virulence in Aspergillus flavus. Currently, methodologies to prevent the negative impact of A. flavus are insufficient. It is known that expression of the hdt5 gene is dependent on hbx1, a master regulatory gene that affects expression of hundreds of genes in the A. flavus genome, controlling both development and secondary metabolism. However, while the aflatoxin gene cluster has been elucidated, the genetic regulatory mechanisms controlled by hdt5 are not fully understood. For this study, therefore, a complete strain set composed of a …

An Exploration Of Social Isolation’S Effects On The Gastrointestinal And Metabolic Health Of A Social Mammal, Daniel Adam Nuccio

Graduate Research Theses & Dissertations

Human health can be impacted considerably by subjective feelings of loneliness, resulting in mental illness and the dysregulation of normal cardiovascular, immune and endocrine functioning. This occurs because social isolation serves as a form of chronic stress for social mammals, spurring physiological responses akin to those associated with more standard forms of stress. On account of their highly social nature, prairie voles (Microtus ochrogaster) subsequently have become one of the model organisms of choice for investigating the effects of social isolation on the development of disease in social mammals.

This dissertation seeks to expand the scientific community’s understanding of the …

Molecular Interactions Between The Shellfish Pathogen Vibrio Coralliilyticus Re22 And The Probiont Phaeobacter Inhibens S4sm, Jessica Coppersmith

Open Access Dissertations

Oyster larvae aquaculture is an important industry, both economically and environmentally, helping to create sustainable food systems by supplying seed oysters to farms, and also to restoration projects aimed at building coastal resilience and improving water quality by rejuvenating wild populations. However, oyster larvae are highly susceptible to rapid mortality events, crashes, which can seriously impact the industry. One source of these crashes in hatchery systems is vibriosis, caused by pathogenic species of Vibrio bacteria that are ubiquitous in temperate coastal waters, and with rising surface water temperatures their prevalence and virulence are increasing. To prevent these larval crashes from …

Effects Of Added Vestibular Noise On Blindfolded Locomotor Veering, Russell J. Carmichael, Allison Sam, Philip K. Schot Phd

International Journal of Undergraduate Research and Creative Activities

BACKGROUND: Nearly every fundamentally healthy human, regardless of fitness, age, or gender is incapable of walking in a perfectly straight line when visual information is removed. This is known as veering. Many different potential causes have been examined, but the most persuasive explanation is that without visual input, sensory interference (or noise) accumulates and causes random motor output errors. OBJECTIVE: The aim of this study was to examine veering behavior during blindfolded walking when added vestibular noise (vibration) was applied to the skull at select locations. METHODS: A handheld massager attached at the top of the head or over one …

Integrating Invasion Risk And Habitat Suitability To Guide Invasive Species Management In Wetlands, Lakes, And Rivers, Brenden M. Elwer

Electronic Theses and Dissertations

Invasive species disrupt ecosystems by altering habitats, competing with native species, and disrupting food webs. The northern Great Plains is an ecologically important region with many wetlands, lakes, and rivers that are under threat from two invasive species, Bighead (Hypophthalmichthys nobilis) and Silver Carp (H. molitrix; bigheaded carp). These large planktivores change the abundance and composition of phytoplankton and zooplankton communities which alters prey for native mussels, fishes, and, potentially, waterfowl. Bigheaded carps have invaded a portion of the Northern Great Plains, and preventing further spread is a primary management goal. My work assists these efforts by providing tools to …

Louisiana Agriculture Magazine, Winter 2025, Lsu Agcenter

Louisiana Agriculture

LSU AgCenter nutrition education programs have always aimed to support the health and well-being of Louisianans. Helping residents eat healthier, be more physically active and maintain a healthy weight are important parts of disease prevention. Building healthy, thriving communities across Louisiana can support reduced healthcare costs, improved workforce productivity and an enhanced quality of life for everyone in our state.

Adaptation For Whom? Understanding The Impacts Of Dyke Policies On Small-Scale Farmers In Ca Mau Province Of Vietnam, Nguyen Minh Quang, Nguyen Van Minh, Nguyen Vo Chau Ngan, Martha B. Lerski

Publications and Research

Low-lying deltas worldwide are extremely vulnerable to sea-level rise. As the risks of climate-induced flooding become increasingly clear, the thirst for infrastructure-driven adaptation increases. This highlights a patent need to understand the impacts of engineering adaptation solutions, especially those targeting built structures such as dykes and sluices, on farmers from a bottom-up, farmer-centred perspective. The study addresses this call by examining cases where dyke policies, while successful in some dimensions, have undermined small-scale farmer livelihoods in Ca Mau ― Vietnam’s southernmost rural province. It identifies areas in need of attention and adds empirical data related to this group of farmers. …

Evaluating The Success Of The Rev It Up! Curriculum Training On The Self-Efficacy Of Sdsu Extension Expanded Food And Nutrition Education Program (Efnep) & Supplemental Nutrition Assistance Program - Education (Snap-Ed) Paraprofessional Educators In Teaching Adolescents, Oluwatemilorun Akinsanya

Electronic Theses and Dissertations

Objective: Evaluate self-efficacy changes among Extension paraprofessional nutrition educators after receiving an adolescent curriculum (Rev It Up!) training. Methods: Paired t-tests on three adapted scales measured changes at pre- (T1), post- (T2), and 1 yr post-intervention (T3): the Teacher Interpersonal Self-Efficacy Scale (TISS), Nutrition Teaching Self-Efficacy Scale (NTSES), and Self-Efficacy and Nutrition Teaching Methods scale (SNTMS). Results: Six educators completed the TISS and NTSES; 5 completed the SNTMS. The TISS showed significant improvement from T1 to T2 only (p= 0.03); the NTSES showed improvement between T1 and T2 (p= 0.01) and T1 and T3 (0.01); and there were no significant …

Ovarian Response, Endocrine Environment, And Pregnancy-Associated Glycoproteins In Beef Cows Using The 7 & 7 Synchronization Protocol, Hunter Haberman

Electronic Theses and Dissertations

This Review aims to provide an overview of the bovine estrous cycle and pregnancy establishment, beginning with follicular development, luteolysis, maternal recognition of pregnancy and early embryonic development. Manipulating the estrus cycle with synchronization protocols is a critical aspect of the beef industry and has greatly improved the efficiency of artificial insemination. This review will evaluate various strategies to synchronize estrus from the very basic to more advanced synchronization protocols. There are many factors that affect the success of the protocol, and many studies have been done to evaluate the estrus cycle to reach the highest level of success from …

Identification Of Host-Pathogen Protein Interactions: Yeast Two-Hybrid Analysis Of Sars-Cov-2 Nsp10 And Human Cellular Proteins, Antonios Argyriou

Selected Full-Text Master Theses 2021-

The ongoing COVID-19 pandemic, caused by SARS-CoV-2, has resulted in over 774 million cases and 7 million deaths worldwide, establishing it as one of the most devastating pandemics in recorded history. While current therapeutic approaches primarily target viral entry mechanisms and specific enzymes, the limited understanding of non-structural protein functions hinders the development of comprehensive antiviral strategies. This research focuses on Nsp10 (non-structural protein 10), a highly conserved 139-amino acid protein essential for viral mRNA capping and replication. Nsp10 serves as a critical cofactor in the formation of methyltransferase complexes with Nsp14 and Nsp16, playing a vital role in viral …

Periconceptual Nutrition And The Preimplantation Embryo: Approaches For Intervention And Generational Health, Abigail Klein

Dissertations and Theses

The typical American diet exceeds dietary fat recommendations, contributing to the rising rates of obesity. This negatively impacts fertility, pregnancy and fetal growth, and lifelong offspring health. During pregnancy, this also increases risk of co-morbidities like gestational diabetes. Despite this, lipids are not screened during pregnancy and limited research exists evaluating these co-exposures on offspring health. Our lab used a rat model of maternal high fat (HF) diet with late-gestation diabetes (DM) to demonstrate that mitochondrial dysfunction and oxidative stress incite lifelong cardiometabolic disease risk in offspring. To determine the timing of damage, we hypothesized that maternal overnutrition incites mitochondrial …

The Design Of A Bioinspired Impact Mitigation System Towards The Prevention Of Brain Injuries, Bryce H. Reimer

Master’s Theses

Traumatic brain injuries (TBIs), including concussions and chronic traumatic encephalopathy (CTE), remain a major concern across contact sports, military operations, and transportation. Despite advancements in protective equipment, current strategies often fall short in preventing the mechanical conditions that contribute to long-term neurological damage. This thesis presents the design and validation of a bioinspired impact mitigation system modeled after bighorn sheep horn structures, which have evolved to endure high-energy collisions without apparent brain injury.

Using biomimetic principles, the research integrates experimental and computational methods to evaluate how horn-like geometries reduce impact-induced accelerations. A custom drop tower was built to test dynamic …

Collaborative Wildfire Governance Within The Southwest Idaho Wildfire Crisis Strategy Landscape: Survey Results 2025, Mahmood Muttaqee, Cody R. Evers, Lindsay K. Campbell, Max Nielsen-Pincus, Olivia Awbrey, Kathryn Hixson, Michelle Johnson, Erika Svendsen

Environmental Science and Management Faculty Publications and Presentations

Collaboration plays a critical role in wildfire risk mitigation efforts. This study examines collaborative wildfire governance networks within Southwest Idaho, a priority landscape designated in 2022 under the USDA Forest Service's Wildfire Crisis Strategy (WCS). Through survey data from 64 respondents, the Fueling Adaptation study aimed to identify the structure, capacity, and gaps in governance networks addressing wildfire risk in Southwest Idaho and understand how WCS investments have influenced wildfire management in the area. The findings reveal a growing governance network centered on the USDA Forest Service and the Idaho Department of Lands and supported by numerous non-governmental organizations and …

Insights Into Female Urinary Microbiota Through Long-Read 16s Rrna Sequencing, Jerrin John

Dissertations

The human urinary microbiome—once thought to be sterile—is now recognized as a dynamic, low-biomass microbial community with potential implications for urinary tract health. Conventional short-read 16S rRNA gene sequencing targeting individual variable regions often lacks the taxonomic resolution needed to distinguish closely related species, while culture-based methods may miss fastidious or low-abundance taxa. We applied full-length 16S rRNA gene sequencing across all nine variable regions to daily urine samples collected from asymptomatic premenopausal female participants. Sequence data were processed with a modified DADA2 pipeline optimized for long reads to generate exact sequence variants. We compared species-level community profiles against both …

Jbs’S Initial Public Offering: Threats To Environmental And Human Rights, Kayla Thompson

Indiana Journal of Law and Social Equality

This paper centers around the decision to be made regarding JBS, the world’s largest meat company, wanting to list shares on the New York Stock Exchange (NYSE), and the environmental and social impacts that could result from the Security and Exchange Commission’s (SEC) approval. JBS has been at the center of various controversies over the last several years, including sourcing cattle from ranches acquired through illegal deforestation, engaging in greenwashing practices, incorrectly reporting greenhouse gas emissions, and engaging in human rights abuses. Many environmental groups have filed complaints with the SEC concerning JBS’s bid to list on the NYSE. The …

The Role Of Horizontal Gene Transfer In Antibiotic Resistance Amidst Prevalent Community And Healthcare-Associated Mrsa Rates, Gabrielle Nascimento

Honors College Theses

In recent decades, cases of community-acquired MRSA (CA-MRSA) and healthcare-associated MRSA (HA-MRSA) have been on the rise. The ability of antibiotic resistance to transfer between microorganisms greatly expands its threat to the effective treatment and containment of bacterial infections. The mecA gene is found on a mobile cassette within the Staphylococcal cell and encodes for PBP2a, an alternate version of penicillin-binding protein (PBP) with less binding affinity for β-lactam antibiotics, increasing resistance to the effects of antibiotics. The goal of this research is to investigate the horizontal transfer of the mecA gene between various Staphylococcus species as well as to …

Methyl-Cpg-Binding Domain As A Protein Interaction Partner In Promoter Regulation And Neurodevelopment Through Evolutionary Expanded Entanglement, Stephanie M. Bilinovich, Surya B. Chhetri, Jackson T. Mitchell, Gage O. Leighton, Vladislav Jdanov, Jacob K. Zieba, Taylor W. Cook, Krysta L. Engel, Robert M. Vaughan, Humza Bhatti, Sadie L. Heeringa, Amy M. Wilstermann, Sophie Vanderweele, Akansha S. Das, Emily C. Sherry, Stephen A. Duncan, Katie L. Uhl, David C. Hinds, Mark Mackiewicz, Sarah K. Meadows, Kimberly M. Newberry, E. Christopher Partridge, Sara G. Cline, C. Joy Shepard, D. Casey Smith, Antonio Bradley, Daniel Vogt, Daniel B. Campbell, Lucas Pozzo-Miller, Caleb P. Bupp, Austin J. Goodyke, Surender Rajasekaran, Rebecca C. Knickmeyer, Benjie Blair

Research, Publications & Creative Work

There is increasing evidence that the methyl-binding domain (MBD) is a protein-protein interaction motif that can function independently of methylated DNA binding. The MBD proteins found throughout plants and invertebrates duplicated into multiple vertebrate DNA and non-DNA-binding members (MBD1, MBD2, MBD3, MBD4, MBD5, MBD6, MECP2, BAZ2A, BAZ2B, SETDB1, andSETDB2). Although many invertebrate species possess MBD proteins that can bind and recognize DNA methylation, the DNA-binding function has been independently lost multiple times, with only minor alterations to the protein interaction residues. The nucleosome remodeling and deacetylase (NuRD) complex, which interacts with MBD2/3 and is colocalized with MBD1/4 ChIP-Seq, is maintained …

Global Patterns And Drivers Of Seagrass Biomass, Net Primary Production And Meadow Structure, Nicole R. Foster, Enric Gomis, Diana I. Montemayor, Simone Strydom, Miguel Angel Mateo, Eduard Serrano, Aurora M. Ricart, Martin Dahl, Ines Mazarrasa, Paulina Cisternas, Harry Whiteley, Elliott J. Bates, Viena Puigcorbé, Uxue Moreda, Mar Truc, Karina Inostroza, Rafael Huertas, Roisin Mccallum, Anna Lafratta, Chanelle L. Webster, Caitlyn M. O'Dea, Nicole E. Said, Carlos M. Duarte, Paul S. Lavery, Oscar Serrano

Research outputs 2022 to 2026

Aim: We decipher global patterns in seagrass growth and structure to advance seagrass science, facilitate multidisciplinary research, and promote effective management strategies and policy development for seagrass habitats. Location: Global. Time period: 1975 to 2024. Major taxa studied: Seagrasses—marine angiosperms. Methods: A global data set on seagrass total biomass, total net primary productivity (NPP), shoot density, and percent cover was compiled across 11 genera. We tested how these variables change across seagrass genera, life-history strategy, and geographic location; and then modelled the environmental drivers (temperature, nutrients, light availability) of the patterns observed. Results: The mean (±SE) total biomass of seagrasses …

Decreasing Food Waste In Home Kitchens By Increasing Food Literacy, Thao Rich

Student Publications

In a world that produces enough food for all of its occupants, a staggering amount of it bypasses empty stomachs and goes into landfills every day. There it sits in a plastic bag, which delays decomposition and increases the harmful greenhouse gas emissions. There are numerous reasons for the inequitable distribution of food ranging from politics to logistics, but the fact is that each person can make a dent in reducing their carbon footprint by simply reducing the amount of food they place in the trash each week. Food waste reduction is a small goal that requires no additional spending, …

Exploring The Role Of Cinema In Architectural Perception Of Biophilic Design., Walaa Abdou Abd Elrazik, Mona Awad Abu El-Enein, Mahmoud M. Saafan

Mansoura Engineering Journal

With rapid urbanization transforming the built environment, biophilic architecture offers a sustainable approach that integrates natural elements to promote well-being and ecological balance. As a powerful storytelling medium, cinema shapes architectural perceptions by depicting both the presence and absence of biophilic principles in future urban landscapes. This study investigates how cinematic depictions of biophilic architecture influence audience understanding and appreciation. Using film content analysis, case studies, and surveys of architectural professionals, this research examines selected film scenes, evaluating the emotional and perceptual impact of biophilic versus non-biophilic environments. Findings indicate that films effectively highlight key biophilic features such as natural …

Adaptive Watermarking Scheme For Enhanced Image Authentication And Restoration, Merna E. Ibrahim, Mohamed G. Abdelfattah, Mohamed A. Mohamed

Mansoura Engineering Journal

Traditional fragile watermarking schemes often struggle to balance tamper detection accuracy, image quality, and processing speed. This paper presents a novel adaptive fragile watermarking scheme that leverages Canny-edge detection to dynamically adjust watermark embedding based on image features. The proposed approach classifies image regions into smooth and dense areas. Larger 4×4 blocks are employed in smooth regions, prioritizing visual quality and processing speed, while smaller 2×2 blocks are used in edge and dense regions to enhance tamper detection accuracy. This adaptive strategy effectively balances competing factors, resulting in superior recovered image quality compared to fixed-block schemes. Experimental results demonstrate the …

Development Of An Examination Hall Malpractice Detection System Using Pose Estimation And Machine Learning, Etinosa Noma-Osaghae, Udoumoh John David, Joshua Sokowonci Mommoh, Olusogo Julius Adetunji, Oyedeji Ayo Isaac

Mansoura Engineering Journal

Examination malpractice remains a critical concern, driving the need for robust and efficient detection systems to uphold academic integrity. This research introduces a novel approach to examination malpractice detection by leveraging pose estimation and machine learning to address key gaps in existing solutions. Current systems often rely exclusively on either pose estimation or object detection, failing to exploit the synergistic potential of combining these techniques. This study bridges this gap by integrating deep learning models with complementary capabilities to enhance detection accuracy, robustness, and coverage. The proposed methodology utilises OpenPose for precise human pose estimation, enabling the detection of body …

Optimization Of Persulfate Activation System Using Ferrous Sulfate For Toc Removal From Real Domestic Wastewater, Mohamed Fouad, Mohamed Mossad, Mohamed Gar Alalm, Mahmoud Samy

Mansoura Engineering Journal

The reuse of treated wastewater is increasingly recognized as a sustainable solution to address global water shortage. This study investigates the effectiveness of a persulfate-based advanced oxidation process (AOP) using sodium persulfate (Na2S2O8) activated by ferrous sulfate (FeSO4) as a tertiary treatment for secondary effluents. Real municipal wastewater was collected from Agouzain Wastewater Treatment Plant (WWTP) in Kafr El-Sheikh, Egypt. A Box Benkhen design (BBD) was employed to optimize three key variables: FeSO4 dose (0.5–2.0 g/L), Na2S2O8 dose (0.5–2.0 g/L), and pH (3.0–9.0), with total organic carbon (TOC) removal as the main response. Under optimized conditions (0.50 g/L FeSO4, 2.00 …

Dr. Eric Angel Ramos - A Shooting Star That Inspired Marine Mammalogists And Beyond (1987 – 2024), Laura J. May-Collado, Jeremy J. Kiszka, Nataly Castelblanco-Martínez, Beth Brady, G. Rieucau, Daniel Gonzalez-Socoloske, Renata Sousa-Lima, Diana Reiss, Macelo Magnasco, Roderic Mast, Wayne Sentman, Joy S. Reidenberg

Faculty Publications

No abstract provided.

Igc Oral Presentation Summary: Xxv Igc Congress, S. Ray Smith, Echo Elizabeth Gotsick, J. R. Weinert-Nelson, Alayna Jacobs, Christopher D. Teutsch, Will Fleming, D. R. Woodfield, James L. Klotz, Jeff Lehmkuhler, Greg S. Halich, Brittany Davis, Brittany Hendrix, Krista Lea

IGC Proceedings (1977-2023)

This paper will give an overview of the XXV International Grassland Congress thematic, keynote, and plenary sessions. The theme of the XXV IGC Congress meeting was “Grasslands for Soil, Animal, and Human Health.” We had many people attending, presenting, and participating in all these sessions. Within a month after the conclusion of the Congress, the proceeding’s papers presented at the meeting will be available on the IGC app. By the end of 2023 all papers will be available for download through any search engine at internationalgrasslands.org.

Characterization Of Cancer Cell Migration Through Brain Vascular Endothelium Model, Malak Taysser Al-Noubani

2025 Fall Honors Capstones Projects - Archive

One of the hallmarks of cancer is increased vessel growth to feed the tumor’s rapid expansion. The newly formed blood vessels are immature and often leaky due to structural and functional abnormalities. This leads to ineffective anti-cancer drug delivery and provides tumor cells with additional opportunities to metastasize. We propose to develop an in vitro vascular endothelium model and introduce aggressive triple-negative breast cancer (TNBC) cells to mimic the tumor environment, utilizing a Transwell migration assay. The impact of TNBC cells on vessel permeability will be assessed by staining for tight junction proteins, and the migratory capabilities of TNBC cells …