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Dairy & Food Science News, March 2025, Department Of Dairy And Food Science

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Cd147 Mediates The Metabolic Reprogramming Of Cancer Associated Fibroblasts Induced By Evs Released By Differentiating Cancer Stem Cells, Filomena Colella, Federica Calapà, Giulia Artemi, Erica Pazzaglia, Rita Colonna, Sara Vitale, Giacomo Lazzarino, Federica Vincenzoni, Micol Eleonora Fiori, Ruggero De Maria, Sara Lucchisani, Giannicola Genovese, Luigi Perelli, Barbara Tavazzi, Alessandro Sgambato, Donatella Lucchetti

Faculty, Staff and Student Publications

Several reports have demonstrated that CD147, an N-glycosylated protein that is exchanged by cells in soluble form or through small extracellular vesicles (sEVs), can promote cancer progression. However, its activity related to EVs in colorectal cancer (CRC) is still not fully understood. Previously, we showed that sEV secretion during CRC stem cell (CR-CSCs) differentiation is partially controlled by CD147, and that CD147-expressing sEVs (sEVs-CD147) activate a signalling cascade in recipient cells, inducing molecular invasive features in CR-CSCs. In the present study, we demonstrated that sEVs-CD147 increase the expression of myofibroblast and activation markers in cancer-associated fibroblasts (CAF). In sEVs-CD147-activated CAF, …

Reducing Igg Accumulation Via Neonatal Fc Receptor (Fcrn) Blockade Relieves Neuropathic Pain, Nathan T Fiore, Kendal F Willcox, Dorsa Dayani, Younus A Zuberi, Cobi J Heijnen, Peter M Grace

Faculty, Staff and Student Publications

Preclinical and clinical studies have established that autoreactive immunoglobulin G (IgG) can drive neuropathic pain. We recently demonstrated that sciatic nerve chronic constriction injury (CCI) in male and female mice results in the production of pronociceptive IgG, which accumulates around the lumbar region, including within the dorsal root ganglia (DRG) and spinal cord, facilitating the development of neuropathic pain. These data raise the intriguing possibility that neuropathic pain may be alleviated by reducing the accumulation of IgG. To this end, we tested whether biologic inhibition or genetic deletion of the neonatal Fc receptor (FcRn) would attenuate mechanical hypersensitivity (allodynia) and …

Robust Automated Method Of Spatial Resolution Measurement In Radiotherapy Ct Simulation Images, Pavel Govyadinov, Rick R Layman, Tucker Netherton, Raymond Mumme, Aaron K Jones, Laurence E Court, Moiz Ahmad

Faculty, Staff and Student Publications

Background: Variation in imaging protocol, patient positioning, and the presence of artifacts can vary image quality in CT images used for radiotherapy planning. Automated methods for spatial resolution (SR) estimation exist but require further investigation and validation for wider adoption.

Purpose: To validated previously existing algorithm for SR estimation and introduce improvements that make it robust to patient positioning, CT protocol, site, and artifacts.

Method: A reference algorithm based on the previous gold standard was recreated and modified to improve robustness. The algorithms were tested on three different datasets: (1) a cylindrical ACR CT QC phantom scanned using a Siemens …

Detection Of Alteration In Carotid Artery Volumetry Using Standard-Of-Care Computed Tomography Surveillance Scans Following Unilateral Radiation Therapy For Early-Stage Tonsillar Squamous Cell Carcinoma Survivors: A Cross-Sectional Internally-Matched Carotid Isodose Analysis, Efstratios Koutroumpakis, Mohamed A Naser, Abdallah Sherif Radwan Mohamed, Salman A Eraj, Andrea Jarre, Jay C Shiao, Mona Kamal, Subha Perni, Jack P Phan, William H Morrison, Steven J Frank, G Brandon Gunn, Adam S Garden, Anita Deswal, Jun-Ichi Abe, David I Rosenthal, Elie Mouhayar, Clifton D Fuller

Faculty, Staff and Student Publications

Aim: This study leveraged standard-of-care CT scans of patients receiving unilateral radiotherapy (RT) for early tonsillar cancer to detect volumetric changes in the carotid arteries, and determine whether there is a dose-response relationship.

Methods: Disease-free cancer survivors (>3 months since therapy and age > 18 years) treated with intensity modulated RT for early (T1-2, N0-2b) tonsillar cancer with pre- and post-therapy contrast-enhanced CT scans available were included. Patients treated with definitive surgery, bilateral RT, or additional RT before the post-RT CT scan were excluded. Isodose lines from treatment plans were projected onto both scans, facilitating the delineation of carotid artery …

Imgn853 Induces Autophagic Cell Death In Combination Therapy For Ovarian Cancer, Anca Chelariu-Raicu, Thanh Chung Vu, Sujanitha Umamaheswaran, Elaine Stur, Pahul Hanjra, Yunah Han, Min Hu, Jerome Lin, Barrett C Lawson, Jinsong Liu, Anil K Sood, Yunfei Wen

Faculty, Staff and Student Publications

FOLR1 is heterogeneously overexpressed in epithelial ovarian cancer. We examined the combined effects of the anti-FOLR1 antibody-drug conjugate (IMGN853) with other drugs, including topotecan, anti-VEGF-A antibody, and olaparib. These findings could contribute to the continued development of IMGN853 in the treatment of ovarian cancer.

Treating Ground Water For Agricultural And Individual Consumption, Gabby Miller, Bronson Scott, Michael Clawson

Biomedical Engineering

This project, conducted in collaboration with Cal Poly seniors and Da Vinci High School students, aims to develop a solar-powered groundwater purification system. This system is designed to provide clean water for agricultural use and individual consumption, addressing the growing concerns of groundwater contamination and accessibility needs. The final prototype integrates a gravity filtration system combined with chemical purification, utilizing aluminum sulfate and chlorine to remove particulates, bacteria, and organic compounds.

The research phase involved an extensive review of groundwater contamination issues, existing filtration technologies, and market analysis. Key design constraints included cost-effectiveness, ease of maintenance, and minimal energy consumption, …

Assessment Of Physiological & Biochemical Changes In Tomato Plants Treated With Plant Growth Promoting Rhizobacteria (Pgpr) Under Salinity Stress Conditions, Mohammed Sharif Alhosani

Theses

Plant growth-promoting rhizobacteria (PGPRs) can effectively mitigate the negative impacts of salt stress on plants. The present study reports the isolation of PGPRs from a high-saline mangrove ecosystem in Abu Dhabi, with isolates exhibiting ACC deaminase (ACCD) activity to reduce stress-induced ethylene, along with additional plant growth-promoting (PGP) characteristics, including phosphate solubilization (P+). A total of 57 salt-tolerant bacterial isolates from the mangrove rhizosphere were initially screened for ACCD and P+ activities. Of these, 24 isolates tested positive for PGP characteristics and were further evaluated for their ability to enhance salt stress tolerance in tomato seedlings. In addition, seedlings inoculated …

Elder Mistreatment Within Stroke Family Caregiving, Carina Katigbak, Wesley R Browning, Sean Savitz, Carolyn E Z Pickering

Faculty, Staff and Student Publications

This secondary data analysis sought to identify characteristics associated with mistreatment among chronic stroke survivors who transition to dementia. We examined baseline data from a multi-time series survey study (n = 453; where caregivers of those with stroke n = 107, and those without stroke, n = 346) on caregiving experiences influencing dementia family caregivers’ abusive or neglectful behaviors. Inferential statistical analysis indicated that baseline mistreatment rates were similar across stroke and non-stroke subgroups, though this finding was not significant. Caregiver depression was significantly associated with mistreatment. Multi-morbidity, prescription medication use, and limited mobility were more common among stroke …

Wmh Contributions To Cognitive Impairment: Rationale And Design Of The Diverse Vcid Study, Charles Decarli, Kumar B Rajan, Lee-Way Jin, Jason Hinman, David K Johnson, Danielle Harvey, Myriam Fornage

Faculty, Staff and Student Publications

As awareness of dementia increases, more individuals with minor cognitive complaints are requesting clinical assessment. Neuroimaging studies frequently identify incidental white matter hyperintensities, raising patient concerns about their brain health and future risk for dementia. Moreover, current US demographics indicate that ≈50% of these individuals will be from diverse backgrounds by 2060. Racial and ethnic minority populations bear a disproportionate burden of vascular risk factors magnifying dementia risk. Despite established associations between white matter hyperintensities and cognitive impairment, including dementia, no study has comprehensively and prospectively examined the impact of individual and combined magnetic resonance imaging measures of white matter …

Qsp Modeling Shows Pathological Synergism Between Insulin Resistance And Amyloid-Beta Exposure In Upregulating Vcam1 Expression At The Bbb Endothelium, Zengtao Wang, Vaishnavi Veerareddy, Xiaojiao Tang, Kevin J Thompson, Sunil Krishnan, Krishna R Kalari, Karunya K Kandimalla

Faculty, Staff and Student Publications

Type 2 diabetes mellitus (T2DM), characterized by insulin resistance, is closely associated with Alzheimer's disease (AD). Cerebrovascular dysfunction is manifested in both T2DM and AD, and is often considered as a pathological link between the two diseases. Insulin signaling regulates critical functions of the blood-brain barrier (BBB), and endothelial insulin resistance could lead to BBB dysfunction, aggravating AD pathology. However, insulin signaling is intrinsically dynamic and involves interactions among numerous molecular mediators. Hence, a mechanistic systems biology model is needed to understand how insulin regulates BBB physiology and the consequences of its impairment in T2DM and AD. In this study, …

The Cure-Comm Framework: A Guide For Infusing Cures With Science Communication, Rebekka Darner, Ebenezer Korkor

Faculty Publications – Biological Sciences

Researchers have employed diverse instructional strategies to impart science communication (SciComm) skills, a cherished 21st-century skill into undergraduate students. We reviewed the pedagogical strategies used in developing SciComm skills in undergraduates. This review revealed that analyzing data and scientific literature, increasing exposure to multiple writing contexts, providing multiple opportunities to present findings orally, and providing feedback are used to teach SciComm skills. Many instructors who implement course-based undergraduate research experiences (CURE) report improved SciComm skills as one of the benefits of CUREs. To decipher points of intersection among CUREs and SciComm pedagogical strategies, we also reviewed the literature on pedagogical …

A High-Lipid Diet Leads To Greater Pathology And Lower Tolerance During Infection, Weston Gerald Perrine, Erin Sauer, Ashley C. Love, Ashley Morris, Johnathan Novotny, Sarah Durant

Biological Sciences Faculty Publications and Presentations

Altered food landscapes contribute importantly to wildlife disease dynamics and may play a critical role in host heterogeneity in disease outcomes through changes in host diet composition. We explored the effects of dietary macronutrient composition on disease pathology and feeding behavior of canaries (Serinus canaria domestica) infected with Mycoplasma gallisepticum (MG). In the first experiment, we provided canaries with isocaloric diets composed of identical ingredients that varied in macronutrient content (high protein or high lipid) then MG- or sham-inoculated birds. In the second experiment, we offered both diets to canaries before and after MG or sham inoculation. In experiment one, …

Current Challenges And Preliminary Morphological Reassessment Of The Genus Chaerilus Simon, 1877 In China (Scorpiones: Chaerilidae), Victoria Tang

Euscorpius

The genus Chaerilus Simon, 1877 in China is preliminarily revised on a morphological basis, derived from the available literature and newly examined specimens. The robustness of several commonly applied species-level diagnostic criteria are evaluated. Ten species are provisionally recognized for China, including one new morphospecies, Chaerilus herta sp. n., described from 9 females, 40 males, and 2 juvenile females collected in Mêdog County. The current study only reveals and resolves several most elementary issues in the taxonomy of Chinese Chaerilus. Further molecular investigations are warranted until topotypes of certain species become available.

Estimated Levels Of Glycated Albumin And C-Reactive Protein/Total Albumin Ratio Might Distinguish Prediabetics Among The Apparently Healthy People And Predict The Vulnerability For Development Of Steatohepatitis And Cardiac Risk., Rizk S. Sarhan, Raafat R Mohammed

BioMedicine

Background: Prediabetes precedes Type-2 diabetes development, is characterized by impaired glucose tolerance (IGT) as judged by the 75-gram oral glucose tolerance test (75-OGTT) and is associated with higher cardiac risk (CR), dyslipidemia, hepatosteatosis (HS) and cancer

Aims: The study aimed to determine the distinguishing ability of estimated levels of glycated albumin (GA) and hemoglobin A1c (HbA1c), and serum C-reactive protein (CRP) and serum total albumin (TA) levels for prediabetic out of apparently healthy subjects.

Methods: IGT was diagnosed if fasting blood glucose (FBG) was 100-125 mg/dl and if /dl subject is normal glucose tolerant (NGT). CR, HS and insulin resistance …

Leveraging Arginine Catabolism To Treat Metabolic Complications, Yiming Zhang

Arts & Sciences Graduate Student Theses and Dissertations

Fasting and caloric restriction are effective to treat patients with pre-diabetes or metabolic dysfunction-associated steatotic liver disease (MASLD), previously known as non-alcoholic fatty liver disease (NAFLD), but intensive lifestyle changes are difficult for many to implement and sustain. Therefore, by identifying and studying fasting-mediated signals, we hope to elicit and convey the therapeutic effects of fasting against MASLD without actual physiological fasting. We discovered a key pathway in the liver through unbiased transcriptomic screening in fasted mice, a novel glucose fasting-activated effector: the amino acid hydrolase, arginase 2 (ARG2). Our previously published data demonstrates that forced hepatocyte-specific Arg2 overexpression is …

Flow Dynamics Of Agricultural Waste Nanofibers: Shear, Temperature, And Oscillatory Insights, Bilge N. Altay, Burak Aksoy, James Atkinson, Christopher Lewis, Carlos Diaz-Acosta, Raymond Francis

Articles

The rheology and fiber size of corn stover (CS) and cleaned cotton gin trash (CGT) cellulose nanofibers (CNFs) were studied including behaviors at both moderate and extremely high shear rates, to simulate industrial processes ranging from mixing and pumping to high-speed coating, printing, and extrusion. Particle size analyzer showed that 99% of CS fibers measured around 226 nm, while 85% of CGT fibers were approximately 143 nm. Both CS and CGT CNFs formed gel-like suspensions, and shear flow tests revealed that all samples exhibited shear-thinning behavior, allowing easy flow under shear forces. Gels with higher solid content (1%) demonstrated extended …

Associations Of Parental Perceived Health With Child Movement Behaviors Within Two-Parent Households, You Fu, Hayley Almes, Nora Constantino, Doria Schmidt, Ryan D. Burns

International Journal of Physical Activity and Health

This study aimed to examine how perceived parental mental and physical health status was associated with child physical activity, screen time, and sleep. Participants were 6- to 17-year-old minors (N = 20,156; 47.9% female) from the 2022 US National Survey of Children’s Health. Dependent variables were children’s physical activity frequency, screen time, and sleep. Independent variables were parents’ physical and mental health status. Using the Actor-Partner Interdependence Model, two adjusted path models were constructed. One for parent mental health associating with parental physical health, another for parent physical health associating with parental mental health–both further associating with child movement behaviors. …

Pskh1 Kinase Activity Is Differentially Modulated Via Allosteric Binding Of Ca2+ Sensor Proteins, Christopher R Horne, Toby A Dite, Samuel N Young, Lucy J Mather, Laura F Dagley, Jared L Johnson, Tomer M Yaron-Barir, Emily M Huntsman, Leonard A Daly, Dominic P Byrne, Antonia L Cadell, Boaz H Ng, Jumana Yousef, Dylan H Multari, Lianju Shen, Luke M Mcaloon, Gerard Manning, Mark A Febbraio, Anthony R Means, Lewis C Cantley, Maria C Tanzer, David R Croucher, Claire E Eyers, Patrick A Eyers, John W Scott, James M Murphy

Children’s Nutrition Research Center Staff Publications

Protein Serine Kinase H1 (PSKH1) was recently identified as a crucial factor in kidney development and is overexpressed in prostate, lung, and kidney cancers. However, little is known about PSKH1 regulatory mechanisms, leading to its classification as a “dark” kinase. Here, we used biochemistry and mass spectrometry to define PSKH1’s consensus substrate motif, protein interactors, and how interactors, including Ca2+ sensor proteins, promote or suppress activity. Intriguingly, despite the absence of a canonical Calmodulin binding motif, Ca2+-Calmodulin activated PSKH1 while, in contrast, the ER-resident Ca2+ sensor of the Cab45, Reticulocalbin, Erc55, Calumenin (CREC) family, Reticulocalbin-3, suppressed PSKH1 catalytic activity. In …

Reviews And Syntheses: Variable Inundation Across Earth’S Terrestrial Ecosystems, James Stegen, Amy J. Burgin, Michelle H. Busch, Joshua B. Fisher, Joshua Ladau, Jenna Abrahamson, Lauren Kinsman-Costello, Li Li, Xingyuan Chen, Thibault Datry, Nate Mcdowell, Corianne Tatariw, Anna Braswell, Jillian M. Deines, Julia A. Guimond, Peter Regler, Kenton Rod, Edward K. P. Bam, Etienne Fluet-Chouinard, Inke Forbrich, Kristin L. Jaeger, Teri O'Meara, Tim Scheibe, Erin Seybold, Jon N. Sweetman, Jianqiu Zheng, Daniel C. Allen, Elizabeth Herndon, Beth A. Middleton, Scott Painter, Kevin Roche, Julianne Scamardo, Ross Vander Vorste, Kristin Boye, Ellen Wohl, Margaret Zimmer, Kelly Hondula, Maggi Laan, Anna Marshall, Kaizad F. Patel

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The structure, function, and dynamics of Earth's terrestrial ecosystems are profoundly influenced by how often (frequency) and how long (duration) they are inundated with water. A diverse array of natural and human-engineered systems experience temporally variable inundation whereby they fluctuate between inundated and non-inundated states. Variable inundation spans extreme events to predictable sub-daily cycles. Variably inundated ecosystems (VIEs) include hillslopes, non-perennial streams, wetlands, floodplains, temporary ponds, tidal systems, storm-impacted coastal zones, and human-engineered systems. VIEs are diverse in terms of inundation regimes, water chemistry and flow velocity, soil and sediment properties, vegetation, and many other properties. The spatial and temporal …

Regulating Chemoresistance And Cancer Stemness: The Cdh17-Yap Pathway In Distinct Cellular States Of Lung Cancer Ctc Clusters, Zujun Que, Dan Qi, Yun Yang, Wang Yao, Jiajun Liu, Yan Li, Yuanyuan Yu, Luyao Wang, Fangfei Li, Ge Zhang, Erxi Wu, Jianhui Tian

Children’s Nutrition Research Center Staff Publications

Background: Drug resistance in metastatic lung cancer significantly contributes to patient mortality. This study explores the role of circulating tumor cells (CTCs), the precursors to metastasis, in driving this resistance. We aim to delineate the unique biological traits of CTC clusters in lung cancer and elucidate the mechanisms underlying their resistance to chemotherapy.

Methods: We used an ultralow adsorption plate to establish a CTC suspension culture system. Comparisons between adherent and suspension cultures of CTC-TJH-01 cells were made via Cell Counting Kit-8 (CCK-8), western blot, immunofluorescence, and flow cytometry assays to evaluate cell proliferation, drug resistance, and cancer stemness. The …

Evaluating The Multifaceted Bioactivity Of Syzygium Aromaticum Essential Oil:The Central Role Of Eugenol, Noureddine Rahim, Chamseddine Derabli, Amina Bramki, Sara Mahdjoub, Sandrine Rup-Jacques, Ghozlane Barboucha, Stephani Hesse, Houssem Boulebd

Turkish Journal of Biology

Background/aim: Syzygium aromaticum L. is a versatile plant traditionally used to treat digestive and respiratory issues, improve oral health, and relieve pain, particularly in regions such as Southeast Asia, South Asia, and parts of the Middle East. Its essential oil (EO), predominantly composed of eugenol, is rich in bioactive compounds. This study aims to clarify the specific contribution of eugenol to the antioxidant, antibacterial, antifungal, and insecticidal activities of S. aromaticum EO by comparing their individual effects. Additionally, density functional theory (DFT) calculations were employed to examine the antioxidant mechanism of eugenol and its role in enhancing the overall activity …

Phytochemical Analysis And Antioxidant, Antimicrobial, And Cytotoxic Activities Ofxanthium Strumarium L. (Asteraceae), Ahmet Beyatli

Turkish Journal of Biology

Background/aim: Medicinal plants are considered an important source of novel antioxidant, antimicrobial, and anticancer agents. The main goal of this study was to define the beneficial properties of various extracts obtained from Xanthium strumarium. Materials and methods: Evaluations were conducted on the total phenolic and flavonoid contents of the extracts. High-performance liquid chromatography and diode-array detection (HPLC-DAD) was used to determine the phenolic profiles of the extracts. DPPH, ABTS, and FRAP assays were used to evaluate the free radical scavenging properties of the X. strumarium extracts. The broth dilution method was used for antimicrobial activity assessments, the MTT assay was …

Aberrant Choroid Plexus Formation Drives The Development Of Treatment-Related Brain Toxicity, Tamara Bender, Esther Schickel, Celine Schielke, Jürgen Debus, David R Grosshans, Marco Durante, Insa S Schroeder

Faculty, Staff and Student Publications

Brain tumors are commonly treated with radiotherapy, but the efficacy of the treatment is limited by its toxicity to the normal tissue including post-irradiation contrast enhanced lesions often linked to necrosis. The poorly understood mechanisms behind such brain lesions were studied using cerebral organoids. Here we show that irradiation of such organoids leads to dose-dependent growth retardation and formation of liquid-filled cavities but is not correlated with necrosis. Instead, the radiation-induced changes comprise of an enhancement of cortical hem markers, altered neuroepithelial stem cell differentiation, and an increase of ZO1+/AQP1+/CLDN3+-choroid plexus (CP)-like structures accompanied by an upregulation of IGF2 mRNA, …

Cover Pages For Tgle Vol. 57 Nos 3 & 4

The Great Lakes Entomologist

Cover Pages for TGLE Vol. 57 Nos 3 & 4

Winter Fungi Of The North Woods, Abraham F. Stone, Lane Cozort, Isabelle Holmstrom, Tara Bal

Open Access Books

A simple field guide to assist in identifying winter fungi in northern hardwood forests. This guide is focused on woody species easily found persisting in winter above snow level and with few look-a-likes found in northeastern and north-central North America. Photos and descriptions are from the Upper Peninsula, Michigan.

Molecular Mechanisms Of Niemann-Pick Type C: Investigating Gene Expression, Lipid Metabolism, And Mitochondrial Dysfunction In Fibroblasts, Sarah Kim

Theses and Dissertations

Niemann-Pick Disease Type C (NPC) is a progressive lysosomal storage disorder characterized by the accumulation of lipids, particularly cholesterol, within cells, leading to severe neurological manifestations and developmental issues. This study employs RNA-seq to compare gene expression profiles between fibroblasts derived from a healthy individual and a patient with the NPC1 mutation. A total of 12,978 genes were analyzed, revealing 191 differentially expressed genes. Functional enrichment analysis disclosed significant associations with biological processes related to development, lipid metabolism, embryonic organ development, vascularization, and neuroinflammation. Additionally, the connection between mitochondrial function and lysosomal impairment was explored, revealing that NPC1 mutant fibroblasts …

The Bacterial Microbiome Modulates The Initiation Of Brain Metastasis By Impacting The Gut-To-Brain Axis, Matteo Massara, Michelle Ballabio, Bastien Dolfi, Golnaz Morad, Vladimir Wischnewski, Eleni Lamprou, Joao Lourenco, Stéphanie Claudinot, Hector Gallart-Ayala, Rui Santalla Méndez, Annamaria Kauzlaric, Nadine Fournier, Ashish V Damania, Matthew C Wong, Julijana Ivanisevic, Nadim J Ajami, Jennifer A Wargo, Johanna A Joyce

Faculty, Staff and Student Publications

Brain metastases (BrMs) are the most common brain tumors in patients and are associated with poor prognosis. Investigating the systemic and environmental factors regulating BrM biology represents an important strategy to develop effective treatments. Toward this goal, we explored the contribution of the gut microbiome to BrM development by using in vivo breast-BrM models under germ-free conditions or antibiotic treatment. This revealed a detrimental role of gut microbiota in fostering BrM initiation. We thus evaluated the impact of antibiotics and BrM outgrowth on the gut-brain axis. We found the bacterial genus Alistipes was differentially present under antibiotic treatment and BrM …

Immune Checkpoint Inhibitors Plus Debulking Surgery For Patients With Metastatic Renal Cell Carcinoma: Clinical Outcomes And Immunological Correlates Of A Prospective Pilot Trial, Sangeeta Goswami, Jianjun Gao, Sreyashi Basu, Daniel D Shapiro, Jose A Karam, Rebecca Slack Tidwell, Kamran Ahrar, Matthew T Campbell, Yu Shen, Alexandro E Trevino, Aaron T Mayer, Alexsandra B Espejo, Christian Seua, Marc D Macaluso, Yulong Chen, Wenbin Liu, Zhong He, Shalini S Yadav, Ying Wang, Priya Rao, Li Zhao, Jianhua Zhang, Sonali Jindal, Nizar M Tannir, Andrew Futreal, Linghua Wang, Padmanee Sharma

Faculty, Staff and Student Publications

Surgical removal of primary tumors reverses tumor-mediated immune suppression in pre-clinical models with metastatic disease. However, how cytoreductive surgery in the metastatic setting modulates the immune responses in patients, especially in the context of immune checkpoint therapy (ICT), is not understood. We report the first prospective, pilot, non-comparative clinical trial (NCT02210117) to evaluate the feasibility, clinical benefits, and immunologic changes of combining three different ICT-containing strategies with cytoreductive surgery or biopsy for patients with metastatic clear cell renal cell carcinoma. Primary safety endpoint of this trial has been met, with 43 patients completing cytoreductive surgery, 36 patients undergoing …

Development Of A Conditional Plasmid For Gene Deletion In Non-Model Fusobacterium Nucleatum Strains, Peng Zhou, Bibek G C, Chenggang Wu

Faculty, Staff and Student Publications

Fusobacterium nucleatum is an opportunistic pathogen with four subspecies: nucleatum (FNN), vincentii (FNV), polymorphum (FNP), and animalis (FNA), each with distinct disease potentials. Research on fusobacterial pathogenesis has mainly focused on the model strain ATCC 23726 from FNN. However, this narrow focus may overlook significant behaviors of other FNN strains and those from other subspecies, given the genetic and phenotypic diversity within F. nucleatum. While ATCC 23726 is highly transformable, most other Fusobacterium strains exhibit low transformation efficiency, complicating traditional gene deletion methods that rely on non-replicating plasmids. To address this, we developed a conditional plasmid system in which …