Digital Commons Network^™

Exploring Soft Polymeric Nanoparticles For Targeted Biomolecule Delivery In Plant Cells, Mohammad Rashid

Theses and Dissertations

Safe and efficient agricultural practices require innovative technologies. Nanotechnology offers promising solutions for advancing plant agriculture by enabling the targeted delivery of biomolecules such as agrochemicals and nucleic acids. This study focuses on developing a safe nanotechnological approach for delivering molecules of interest into plant cells. We investigated the ability of four types of polymeric nanoparticles such as Polyethylene glycol N, Nʹ-dibutylethylenediamine (PEG-DB), Polyethylene glycol 2-pyrrolidin-1-yl-ethyl-amine (PEG-PY), Polyethyleneimine (PEI), Arabinoxylan (AX)+, and Zein+ to form conjugates with plasmid DNA (pDNA). Our findings confirm that plant-derived cationic nanopolymers, AX+ and Zein+, effectively bind negatively charged pDNA (pGFPGUSPlus) carrying a GFP reporter …

Mutation Of Smarca4 Induces Cancer Cell-Intrinsic Defects In The Enhancer Landscape And Resistance To Immunotherapy, Yawen Wang, Ismail M Meraz, Md Qudratullah, Sasikumar Kotagiri, Yanyan Han, Yuanxin Xi, Jing Wang, Kadir C Akdemir, Jack A Roth, Yonathan Lissanu

Faculty, Staff and Student Publications

Cancer genomic studies have identified frequent alterations in genes encoding components of the SWI/SNF chromatin remodeling complex, including SMARCA4 and ARID1A. Importantly, clinical reports indicate that SMARCA4-mutant lung cancers respond poorly to immunotherapy and have dismal prognosis. In this study, we corroborated the clinical findings by using immune-humanized, syngeneic, and genetically engineered mouse models of lung cancer harboring SMARCA4 deficiency. Specifically, models with SMARCA4 loss showed decreased response to anti-PD-1 immunotherapy associated with significantly reduced infiltration of dendritic cells and CD4+ T cells into the tumor microenvironment. SMARCA4 loss in tumor cells led to profound downregulation of STING1, IL1β, and …

Isolating Novel Enzymes And Microorganisms For Use In Food Waste Fermentation, Shannon Marie Jeffries

Tennessee State University Alumni Theses and Dissertations

In the United States, upwards of 2 billion pounds of food waste are discarded yearly, an amount equivalent to $161 billion or 30-40% of all food production, making up the largest portion of landfill waste (Buzby, 2022). A small amount of food scraps are recycled through compost and re-purposed into animal feed, but these processes are costly, have minimal benefits, and have limitations (EPA, 2024). One of the most promising routes is converting food waste into value added products through microbial fermentation. Food waste is rich in carbohydrates, proteins, lipids, vitamins, and other trace minerals, making it the ideal environment …

Covid-19 Pandemic And Recovery Experiences: A Case Study With Montana Family And Consumer Science Cooperative Extension Agents, Hayley M. Somerfeld, J. Mitchell Vaterlaus, Nicole C. Wanago, Brianna Routh, Alison Brennan

Journal of Human Sciences and Extension

The present study examined the impact of COVID-19 and recovery experiences among Family Consumer Science (FCS) Extension Agents in Montana. All participants (n = 10) completed a survey with fixed-item and open-ended responses, and six also participated in in-depth interviews. A deductive qualitative case study approach was used. Agent experiences were analyzed using The Sendai Framework for Disaster Risk Reduction (SFDRR) four action priorities: (1) understanding risk, (2) strengthening disaster risk governance, (3) investing in disaster risk reduction for resilience, and (4) enhancing disaster preparedness for effective response and to “build back better” in recovery, rehabilitation, and reconstruction. Pandemic risks …

Empowering Community Through Extension: Identifying Community Health And Wellness Strengths And Needs, Allyson A. Spears, Alexis Zickafoose, Emily Wintermute, Peng Lu, Matthew Baker, Scott Cummings

Journal of Human Sciences and Extension

Health and wellness are foundational to thriving communities, yet many Americans face barriers that impact their access to essential health education and services. This study used a community-based participatory research (CBPR) approach to assess the perceived strengths and needs of health and wellness resources of rural and urban Texas communities. Data were collected through a statewide survey (n = 9,418), targeting key aspects of health and wellness. The results identified critical strengths, including school lunch programs and grocery stores accepting electronic benefit transfers (EBT), while reducing obesity and addressing mental health emerged as top community needs in both urban and …

Discovery Of Alcohol Dehydrogenase (Adh) As A Potential Vaccine Target From Mycolicibacterium Smegmatis Extracellular Vesicles Via Immunoproteomics, M. Jayaram, N. Othman, Khayriyyah Mohd Hanafiah, M. Azlan, N. Arifin

Biology Faculty/Staff Publications

Mycobacterium tuberculosis (MTB), the causative agent of tuberculosis, releases extracellular vesicles (EVs) that impair macrophage functions and circulate bacterial components to modulate the host immune response. While EVs are increasingly investigated as new vaccines and biomarkers, studying MTB EVs is challenging due to the slow growth rate and pathogenic properties of MTB. Mycolicibacterium smegmatis (MSMEG), a non-pathogenic surrogate with a faster growth rate, offers a safer and more convenient option for laboratory studies due to its similarities to MTB. In this study, we explore the antigenic properties of MSMEG EVs to assess their potential use in developing safer tuberculosis vaccine …

Subalpine And Alpine Plant Community Turnover In Yosemite National Park Following 30 Years Of Climate Warming, Rachel Friesen

Master's Theses

Subalpine and alpine regions in California are warming more rapidly than lower elevations. In response, high-elevation plants may acclimate or adapt to the new climate, move to higher elevations or latitudes, persist in refugia, or go extinct. While models predict substantial declines in subalpine and alpine plant communities due to discontinuous population distributions and lack of upslope area, actual responses may vary due to lags in dispersal, establishment, and extinction. Although shifts in high-elevation plant communities have been documented in other regions of the world, such changes have not yet been studied in Yosemite National Park. To assess high-elevation plant …

Antimicrobial Activity In Auxin-Induced Formation Of In Vitro Culture- Derived Aloe Vera Roots, Eufrocinio Marfori, Bernadette Rapiz

The Philippine Agricultural Scientist

Aloe vera is a perennial, succulent plant which has been used in folk medicine for wound-healing, as its roots have been reported to exhibit remarkable antimicrobial properties. However, collecting the roots is detrimental to plant growth and development. Hence, an alternative system of producing A. vera roots must be developed. Tissue culture is a potential tool to obtain A. vera root biomass, which can serve as raw materials for the large-scale production of antimicrobial compounds. In this study, individual shoots taken from the multiple shoot culture of A. vera were induced to form roots in MS medium supplemented with 1 …

Isolation And Enumeration Of Salmonella Enterica From Fresh Lettuce (Lactuca Sativa L.) Leaves And Its Susceptibility To Different Washing Protocols, Meinard Talplacido, Angella Melissa Carlos, Bryan Rey Oliveros, Ida Dalmacio

The Philippine Agricultural Scientist

Lettuce is one of the most consumed fresh produce items in the country, but it also serves as a vehicle for human pathogens, posing a risk of foodborne illness. This study used conventional microbiological and molecular analyses to obtain the concentration and prevalence of Salmonella enterica in fresh Green Ice and Iceberg lettuce leaves from selected supermarkets in Los Baños, Laguna, Philippines. The efficacy of different household washing procedures in reducing S. enterica on lettuce leaves was also investigated. Of the 19 composite Green Ice lettuce and 21 composite Iceberg lettuce samples tested, 5.26% and 4.76%, respectively, were contaminated with …

Growth And Survival Of Silver Therapon (Leiopotherapon Plumbeus [Kner, 1864]) Larvae Fed With Artemia Franciscana Enriched With Isolated Endogenous Probiotic Bacteria, John Carlo Unido, Precious Dee Tolentino, Christian Paul Dela Cruz, Carmela Jhoy Dy, Honey Grace Samonte

The Philippine Agricultural Scientist

This study aimed to isolate, characterize, and identify endogenous lactic acid bacteria (LAB) from the gut of silver therapon (Leiopotherapon plumbeus) and to evaluate their potential as probiotics by enriching live food organisms, specifically brine shrimp (Artemia franciscana), and feeding them to L. plumbeus larvae. Three unknown bacterial isolates were screened and characterized as catalase-negative, Gram-positive, rod-shaped bacteria that showed tolerance to pH and bile, growth at temperatures of 30 and 40 °C, and inhibitory activity against pathogenic bacteria in vitro. The selected isolate was then confirmed as Lactobacillus sp. through partial sequencing of its 16S …

Nebline, June/July 2025

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

4-H embryology will crack 50 years of chicks hatching in local classrooms, Vicki Jedlicka

Simple ways to eat more fruits and veggies every day, Alyssa Havlovic

Recipe of the month: Crunchy veggie wraps

Annual flowers for sunny locations, Mary Jane Frogge

Garden guide: Things to do this month, Mary Jane Frogge

2025 Gro Big Red

It’s time for private well maintenance, Becky Schuerman

Monitoring pastures for improving management and productivity, John Nelson

Why play is your child's most important work, Hayley Jackson Perez

Keep an eye out for some common caterpillars, Kait Chapman

Lancaster County Super Fair

Upcoming babysitting trainings …

A Vacuolar Proton Pump Controls The Post-Germinative Growth Of Rice (Oryza Sativa Ssp. Japonica), Peter James Icalia Gann, Soumen Nandy, Rodrigo Botelho, Kari Vinzant, Mariya Khodakovskaya, Vibha Srivastava

Crop, Soil and Environmental Sciences Faculty Publications and Presentations

Post-germinative growth in plants is controlled by sucrose mobilization from the endosperm. Regulation of this process, however, is not fully understood. Transcriptional suppression of the rice vacuolar H+ translocating pyrophosphatase (V-PPase) referred to as VPP5 led to enhanced starch content in mature grains. However, vpp5 suppressed lines displayed a slower post-germinative growth rescuable by sucrose, indicating inefficient sucrose mobilization from the endosperm. Biochemical and gene expression analyses showed a slower rate of starch to sucrose conversion and altered expression of the key starch utilization pathway genes. We hypothesized that the modulation of metabolic activities in the vpp5 lines was based …

Exploring Natural Competence Of Escherichia Coli Through Biofilm Formation, Thulni Liyanaarachchi

University Honors Theses

Escherichia coli is a species not known for natural competence; however, specific growth conditions can induce the process in this bacterium due to the presence of homologous competence genes. These conditions could be met with biofilm formation through an active growth phase in the two-layer architecture. Therefore, my thesis examined whether natural competence is exhibited in E. coli during biofilm formation as determined by the genetic transfer of ampicillin and chloramphenicol-resistance genes between two separate strains. The experiments were broken into three aims to determine the morphology of the biofilm in inter-strain co-cultures and cell-DNA mixes, the effect of …

A Review On The Cytogenetic Integrity Of Micropropagated Plants [Review Paper], Eliaza Alumbro Peniton Jr

The Philippine Agricultural Scientist

Cytogenetic stability is essential for the survival of micropropagated plants. While micropropagation allows rapid propagation of plants with desirable phenotypes, it may also produce genetic variations that are, at times, cytogenetically observable. Factors affecting these variations are not fully understood, and micropropagation protocols are optimized to produce genetically stable regenerants. The genetic stability of micropropagated plants is traditionally assessed by comparing the phenomorphological traits of the regenerants to the mother plant. However, genetic variation does not often result in obvious morphological changes. Therefore, molecular and cytogenetic markers are typically used to evaluate the genetic variations of regenerants. Moreover, analyzing plant …

West Coast Demersal Scalefish Resource Recovery Support Package: 2023-24 Report, Department Of Primary Industries And Regional Development, Western Australia

Fisheries Published Reports

The West Coast Demersal Scalefish Resource (WCDSR) includes over 100 species in inshore (20–250 m deep) and offshore ( > 250 m) demersal habitats of the West Coast Bioregion. These species are caught by charter, commercial and recreational fishers.

The WCDSR is in recovery and managed under a 20-year recovery plan (2010–2030). DPIRD tracks recovery progress through scientific stock assessments of the indicator species (snapper, WA dhufish, and baldchin groper) every 3 years.

The 2021 WCDSR stock assessment showed limited recovery and additional management action was required.

In December 2022, the Minister for Fisheries announced commercial and recreational (including charter) sector …

Correlations Between Social Media, Body Image Perceptions, And Disordered Eating Prevalence In Postpartum Women: A Pilot Cross-Sectional Study, Hannah Smith

Master's Theses

Postpartum women are a high-risk population for body dissatisfaction and disordered eating behavior due in part to the plethora of hormonal and physiological changes. Despite this, research examining this issue among postpartum women remains limited.

This pilot cross-sectional study investigated the relationship between social media use, body dissatisfaction, and disordered eating behavior among women up to 13 months postpartum. For this study, the following research questions were asked: Is high social media usage associated with higher levels of disordered eating among women up to 13 months postpartum? Is the association between social media usage and disordered eating mediated by a …

Understanding How Genetic Mutations Induce Oligodendrocyte Progenitors To Become Cancer Cells, Dennis Huang

Dissertations, Theses, and Capstone Projects

Gliomas are the most devastating adult brain tumors characterized by poor survival rate and limited options for treatment. Previous studies have shown that they are very heterogeneous and can be further sub-classified based on their transcriptional signature and the presence of specific mutations. One such subtype, is the “proneural glioma”, which is characterized by the enrichment in oligodendrocyte progenitor cell (OPC) transcripts and mutations in genes encoding for the tumor suppressor P53 (Trp53) and for Platelet Derived Grow Factor (PDGF) signaling. Since OPCs are the most abundant proliferative population in the adult brain, in this …

The Burden Of Burnout: Understanding Its Prevalence And Organizational Drivers In Medical Physics, Deborah Schofield, C Lynn Chevalier, Laurence Court, William Harmsen, Akiva Turner

Faculty, Staff and Student Publications

Background: Burnout is a work-related syndrome characterized by increased levels of emotional exhaustion (EE) and depersonalization (DP) along with decreased levels of personal achievement. In the healthcare setting, higher burnout levels have been associated with negative impacts on personnel, an increased risk of errors, and a decrease in the quality of delivered care.

Purpose: The purpose of this study was to assess the prevalence of burnout among medical physicists working in the United States. Additionally, the impact of personal and organizational features on burnout risk was examined.

Methods: The anonymous survey was distributed to 1962 full members of the American …

Impact Of Screening For Human Papillomavirus-Positive Oropharyngeal Cancers: A Microsimulation-Based Modeling Study, Rebecca Landy, Gregory W Haber, Barry I Graubard, Carole Fakhry, Nicole G Campos, Emily A Burger, Li C Cheung, Hormuzd A Katki, Maura L Gillison, Anil K Chaturvedi

Faculty, Staff and Student Publications

Background: We estimated the impact of screening on morbidity and mortality of human papillomavirus (HPV)16-positive oropharyngeal cancer among US men aged 45-79 years.

Methods: We developed an individual-level, state-transition natural history microsimulation model to estimate the impact of screening using oral HPV16 detection, HPV16-E6 antibody detection, and transcervical ultrasound of neck/oropharynx. We compared clinical detection to counterfactual screen detection for cancer stage, single- vs multiple-modality treatment, and survival. Screening scenarios encompassed 4 progression speeds across cancer stages (very slow, slow, fast, and very fast) and 4 screening frequencies.

Results: Among US men aged 45-79 years in 2021 (n = 54 …

Building Community Through Data: The Value Of A Researcher Driven Open Science Ecosystem, Meredith C B Adams, Carla M Bann, Emine Ozgur Bayman, Maria Chao, Georgene W Hergenroeder, Charles Knott, Martin A Lindquist, Z David Luo, Rosemarie Martin, Maryann E Martone, John Mccarthy, Micah Mccumber, Sharon B Meropol, Ty A Ridenour, Lissette M Saavedra, Abeed Sarker, Kevin J Anstrom, Wesley K Thompson

Faculty, Staff and Student Publications

No abstract provided.

Multicancer Early Detection Tests At A Crossroads: Commercial Availability Ahead Of Definitive Evidence, Carmen E Guerra, Jennifer Keating Litton, Carolina E Viswanath, A Mark Fendrick

Faculty, Staff and Student Publications

Multicancer early detection tests (MCEDs), sometimes referred to as liquid biopsies, are tests that can screen for multiple cancers by analyzing blood, urine, and other bodily fluids for biomarkers released by cancer cells. These tests have the potential to change the cancer screening paradigm if they are shown to reduce cancer mortality. However, it is not yet known whether MCEDs reduce mortality. Randomized controlled trials, the gold standard for evaluating cancer screening programs, are currently evaluating the effectiveness of MCEDs. However, because cancer-specific and all-cause mortality are end points that can take years to reach, trials are being designed with …

A Gut-On-A-Chip Incorporating Human Faecal Samples And Peristalsis Predicts Responses To Immune Checkpoint Inhibitors For Melanoma, Mattia Ballerini, Serena Galiè, Punit Tyagi, Carlotta Catozzi, Hariam Raji, Amir Nabinejad, Angeli D G Macandog, Alessandro Cordiale, Bianca Ionela Slivinschi, Karol K Kugiejko, Martina Freisa, Paola Occhetta, Jennifer A Wargo, Pier F Ferrucci, Emilia Cocorocchio, Nicola Segata, Andrea Vignati, Andrey Morgun, Michela Deleidi, Teresa Manzo, Marco Rasponi, Luigi Nezi

Faculty, Staff and Student Publications

Patient responses to immune checkpoint inhibitors can be influenced by the gastrointestinal microbiome. Mouse models can be used to study microbiome-host crosstalk, yet their utility is constrained by substantial anatomical, functional, immunological and microbial differences between mice and humans. Here we show that a gut-on-a-chip system mimicking the architecture and functionality of the human intestine by including faecal microbiome and peristaltic-like movements recapitulates microbiome-host interactions and predicts responses to immune checkpoint inhibitors in patients with melanoma. The system is composed of a vascular channel seeded with human microvascular endothelial cells and an intestinal channel with intestinal organoids derived from human …

Nlrp3 Inflammasome Activation Expands The Immunosuppressive Myeloid Stroma And Antagonizes The Therapeutic Benefit Of Sting Activation In Glioblastoma, Spencer T Lea, Chao-Hsien Chen, Jun Wei, Ivana William, Inés Lopez Del Castillo, Michael A Curran

Faculty, Staff and Student Publications

Glioblastoma (GBM) is the most common and deadly primary brain malignancy and is clinically refractory to immunotherapy. Active NLRP3 inflammasome signaling and IL-1β secretion have been observed in GBM, and NLRP3-driven myeloid-derived suppressor cell (MDSC) recruitment can mediate cancer immune evasion. Agonists of the cytosolic double-stranded DNA-sensing stimulator of IFN gene (STING) pathway can mediate proinflammatory conversion of cancer MDSCs; however, secretion of the NLRP3 products IL-1β and IL-18 has also been observed in certain myeloid populations following STING activation. In this study, we aimed to determine both the potential mechanistic synergy between STING and NLRP3 agonists, and the effects …

Targeted Sting Activation Using Modified Ultrasound-Responsive Microbubbles Enhances Immune Checkpoint Blockade Against Melanoma, Sina Khorsandi, Kristin Huntoon, Yifan Wang, Adam Woodward, Abin Antony, Connor Endsley, Nazia Hafeez, Jared L Edwards, Nicole Mccuen, Prasanna G Alluri, Betty Y S Kim, Wen Jiang, Jacques Lux

Faculty, Staff and Student Publications

Despite the recent successes of immune checkpoint inhibitors (ICIs) in treating advanced melanoma, durable clinical responses still remain limited. To boost immune responses, agents that target immune regulators, such as the Stimulator of Interferon Genes (STING) agonist cyclic GMP-AMP (cGAMP), are being investigated. However, their clinical translation is impeded by poor serum stability, rapid tissue clearance, and T-cell death due to off-target activation. Recently, a novel strategy termed Microbubble-assisted UltraSound-guided Immunotherapy of Cancer (MUSIC) has been reported to selectively deliver cGAMP directly into the cytosol of antigen-presenting cells with spatiotemporal control. The resulting activation of STING and downstream proinflammatory pathways …

Comparing Stroke Risk Factors Among Sexual Minority Groups In Texas, Brittany Krenek, Samuel Tundealao, Jennifer E S Beauchamp, Sean I Savitz, Irene Tamí-Maury

Faculty, Staff and Student Publications

Background: Knowledge gaps remain on stroke risk and disparities between sexual minority (SM) subgroups. In this study, stroke risk between SM subgroups, specifically gay/bisexual men and lesbian/bisexual women (G/BM and L/BW), was assessed.

Method: Data were collected in June 2022 using a bilingual (English and Spanish) cross-sectional paper-and-pen survey distributed among 183 SM individuals attending the 2022 Houston Pride Parade and Festival, as well as across Texas via phone call or online format. Relevant sociodemographic and stroke risk factors were compared between G/BM and L/BW using chi-square (or Fisher's exact, when appropriate) and two-sample t-tests. Sexual orientation was used to …

Electrospun Membranes From Bio-Renewable Poly (Ethylene Furanoate)/Poly (Ethylene Teraphthalate) (Pef/Pet) Blend For Emulsification Application, Aya Ismat Sayed

Theses

This thesis focuses on manufacturing electrospun fibrous membranes from a blend of poly (ethylene terephthalate) and poly (ethylene furanoate) and using in premix emulsification. Poly (ethylene furanoate), which is a 100% bio-renewable polymer being blended with synthetic poly (ethylene terephthalate) for membrane fabrication contributes towards sustainability in the production of food and pharmaceutical emulsions. The primary objective of this research is to optimize the fabrication conditions of poly (ethylene terephthalate)/poly (ethylene furanoate) porous membranes, characterize their properties, and evaluate their performance in emulsification. This study compares poly (ethylene terephthalate) / poly (ethylene furanoate) blend membranes with individual poly (ethylene terephthalate) …

Cohabitation At Threshold: Carpenter House Reimagined For Multispecies Living, Zhixiao Yang

Masters Theses

With the rapid growth of the human population, we are encroaching further into wildlife habitats, particularly those surrounding urban environments. This accelerating urbanization leads to biodiversity loss, the degradation of ecological services essential to human health and well-being, and a diminishing connection between city dwellers and the natural world.

Today, urban planners, architects, economists, and other advocates for the future of humanity and the health of the planet are actively questioning how we might build greener, more resilient cities. While much of the focus has been on large-scale interventions — such as the use of sustainable materials, the creation of …

Covered In Snow, Katelynn M. Rogers

Masters Theses

Covered in Snow is a photographic exploration into the ripple effect of maternal loss and environmental decay in my hometown of Canton, Ohio. In the wake of a miscarriage, the woods I had grown up within began calling to me, urging me to return. Unbeknownst to me, a series of accidental deaths of women had been happening throughout these trees my entire life, their stories only coming to the surface as I looked to the trees for solace. This series of inexplicable deaths in the woods I so desperately loved began to alter my relationship to the trees, a distrust …

Field Journal: The Excluded Middle, Carrie E. Kouts

Masters Theses

What does it look like to engage with organisms and landscapes at the periphery of anthropocentric value structures? How does one break the internalized myth that the “built” environment is excluded from the natural world? When does a hyper-mobile and hyper-commodified society confront the exponential crisis of animal death? Within this series of journal entries, field notes, collection observations, weird prose, and sensory musings, one will find questions on the nature of being human and the complex narratives of care we encounter in a world shared with more-than-humans. Each handwritten vignette and photograph from daily life weaves a non-linear and …

Genetic Factors Of Campylobacter Jejuni Required For Its Interactions With Free-Living Amoeba, Deepti Pranay Samarth, Asim Z. Abbasi, Young Min Kwon

Poultry Science Faculty Publications and Presentations

Acanthamoeba, a free-living amoeba ubiquitous in environmental water, has been considered as the environmental reservoir of certain bacterial pathogens, including Campylobacter jejuni, an intracellular human pathogen causing self-limiting gastroenteritis. Acanthamoeba-C. jejuni interaction mechanisms may help clarify how the otherwise fastidious bacterium C. jejuni survives in environmental waters. In this study, we constructed single deletion mutants of C. jejuni strain 81-176 for the 10 selected genes (motAB, ciaB, kpsE, virB11, cheY, flaAB, cstII, docB, sodB, and cadF) previously shown to be important for the interaction (invasion and intracellular survival) of C. jejuni with mammalian hosts. We used a modified gentamicin protection …