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Articles 1651 - 1680 of 39817
Full-Text Articles in Entire DC Network
Proteomic Approaches To Investigate Pp2a-B55 Substrates And Cellular Function, Brennan C. Mcewan
Proteomic Approaches To Investigate Pp2a-B55 Substrates And Cellular Function, Brennan C. Mcewan
Dartmouth College Ph.D Dissertations
Cell division is controlled in large part by reversible protein phosphorylation. The phosphorylation state of proteins during mitosis and other phases of the cell cycle represents a careful balance of activity between kinases and opposing phosphatases. The phosphoprotein phosphatase (PPP) family is responsible for the majority of serine and threonine dephosphorylation. Members of the PPP family are composed of a catalytic subunit paired with a regulatory subunit that targets them towards specific substrates.
The most abundant PPP, PPP2A, paired with its regulatory B55 subunit, is thought to be the phosphatase responsible for the dephosphorylation of substrates during mitotic exit. Despite …
Hcmv Infection Of Terminally Differentiated Neurons Disrupts Microtubule Organization, Resulting In Neurite Retraction, Jacob W. Adelman, Andrew T. Sukowaty, Kaitlyn J. Partridge, Jessica E. Gawrys, Allison Akins, Scott S. Terhune, Allison D. Ebert
Hcmv Infection Of Terminally Differentiated Neurons Disrupts Microtubule Organization, Resulting In Neurite Retraction, Jacob W. Adelman, Andrew T. Sukowaty, Kaitlyn J. Partridge, Jessica E. Gawrys, Allison Akins, Scott S. Terhune, Allison D. Ebert
Biological Sciences Faculty Research and Publications
Human cytomegalovirus (HCMV) is a prolific human herpesvirus that infects most individuals by adulthood. While typically asymptomatic in adults, congenital infection can induce serious neurological symptoms, including hearing loss, visual deficits, cognitive impairment, and microcephaly in 10%–15% of cases. HCMV has been shown to infect most neural cells, with our group recently demonstrating this capacity in stem cell-derived forebrain neurons. Infection of neurons induces deleterious effects on calcium dynamics and electrophysiological function paired with gross restructuring of neuronal morphology. Here, we utilize an induced pluripotent stem cell-derived model of the human forebrain to demonstrate how HCMV infection induces syncytia, drives …
Functional Analysis Of R144 In The Flavin Oxidation Mechanism Of A Flavin-Dependent N-Monooxygenase, Reeder Robinson, Korliss Britt, Pablo Sobrado
Functional Analysis Of R144 In The Flavin Oxidation Mechanism Of A Flavin-Dependent N-Monooxygenase, Reeder Robinson, Korliss Britt, Pablo Sobrado
Chemistry Faculty Research & Creative Works
Flavin-dependent N-monooxygenases (NMOs) are key enzymes in the biosynthesis of hydroxamate-containing siderophores, which are critical virulence factors in pathogenic microorganisms. SidA, an NMO from Aspergillus fumigatus, catalyzes the NADPH- and O2-dependent hydroxylation of L-ornithine (Orn) via a stable C4a-hydroperoxyflavin (FADOOH) intermediate. This study investigates the role of Arg144 in the catalytic cycle of SidA. Site-directed mutagenesis of Arg144 to alanine (R144A) significantly impaired both oxygen consumption and Orn hydroxylation, resulting in an approximately 60-fold reduction in kcat and a ~300-fold decrease in kcat/KM. The pH dependence, solvent kinetic isotope effects, and rapid-reaction kinetics reveal that R144 influences protonation events crucial …
Antibiotic Resistance Trends In Coliform Bacteria Of Kittitas County Waters, Kaitlyn Holbrook, Bella Blasingim, Cruz Veronica Guillen Mejia, Holly Pinkart
Antibiotic Resistance Trends In Coliform Bacteria Of Kittitas County Waters, Kaitlyn Holbrook, Bella Blasingim, Cruz Veronica Guillen Mejia, Holly Pinkart
Journal of the Symposium of University Research and Creative Expression
Project Mentor(s): Holly Pinkart, PhD
Since the mid-1900s, antibiotics have been developed to help fight against bacterial pathogens. However, bacteria can evolve rapidly and have evolved resistance to many types of antibiotics. Using coliform bacteria collected from waters around Kittitas County, BIOL 323 students at Central Washington University have identified a variety of antibiotic-resistant bacteria in Kittitas County streams and canals. Antibiotic resistance was measured from 2014-2019 and compared to data collected in 2024. This study focuses on three antibiotics, ampicillin, kanamycin, and trimethoprim-sulfamethoxazole. In the past (2014-2019) we saw no change in the trend of antibiotic resistance in collected …
Characteristics Contributing To The Risk Of Diabulimia In Adults Living In The United States, Danielle Van Hout, Nicole Stendell-Hollis, Tafere Gebreegziabher Belay, Kelly Pritchett
Characteristics Contributing To The Risk Of Diabulimia In Adults Living In The United States, Danielle Van Hout, Nicole Stendell-Hollis, Tafere Gebreegziabher Belay, Kelly Pritchett
Journal of the Symposium of University Research and Creative Expression
Project Mentor(s): Nicole Stendell-Hollis, PhD, RDN; Tafere G Belay, PhD; Kelly Pritchett, PhD, RDN, CSSD
The purpose of this study was to determine the prevalence and factors that contribute to the risk of diabulimia by using the validated Diabetes Eating Problem Survey-Revised (DEPS-R)1 screening tool in adults living in the U.S., as well as to examine differences in individuals at-risk and not-at-risk for diabulimia. This observational study included a 30-question Qualtrics survey containing the DEPS-R distributed via various social media outlets and flyers on the CWU Ellensburg campus. Participants were required to be 18 years or older, diagnosed with …
Public Perceptions Of Universities As Public Entities During The Covid-19 Pandemic Era, Jinho Jung, Laura Barrett, Nicole Olynk Widmar, Mario Ortez
Public Perceptions Of Universities As Public Entities During The Covid-19 Pandemic Era, Jinho Jung, Laura Barrett, Nicole Olynk Widmar, Mario Ortez
Department of Agricultural Economics Faculty Publications
Higher education institutions are facing challenges in navigating social, cultural, and political issues. The online presence of higher education presents a new set of opportunities and challenges; knowledge, news, and continuing education are increasingly disseminated online. Additionally, the public, student bodies and stakeholders are increasingly looking at reviews or seeking opinions via online and social media to form opinions on institutions. This analysis uses online and social media to analyze and compare perceptions of two public universities in Indiana, namely Purdue University and Indiana University. These two Indiana institutions are located in the same state, allowing for comparisons of public …
Decorin Evokes A Pro-Lysosomal Pathway In Lymphatic Endothelial Cells, Gabriel J. Pascal, Dipon K. Mondal, Sadie Kim, Christopher Xie, Devanarayanan Siva Sankar, Jörn Dengjel, Renato V. Iozzo
Decorin Evokes A Pro-Lysosomal Pathway In Lymphatic Endothelial Cells, Gabriel J. Pascal, Dipon K. Mondal, Sadie Kim, Christopher Xie, Devanarayanan Siva Sankar, Jörn Dengjel, Renato V. Iozzo
Department of Pathology, Anatomy, and Cell Biology Faculty Papers
The lymphatic system is critical to the body's immune and circulatory system, and lymphangiogenesis, the development of new lymphatic vessels from pre-existing ones, is a significant process capitalized upon by cancer during tumorigenesis. Decorin is a small leucine-rich proteoglycan which we have previously shown to be anti-tumorigenic and a suppressor of lymphangiogenesis. We have also shown that decorin exercises its anticancer properties through its ability to evoke autophagy. Through a comprehensive and unbiased proteomic analysis, we explored the implications of decorin exposure on protein expression within mouse lymphatic endothelial cells. We discovered that decorin enriches several protein pathways, notably proteasomal …
Clinical And Genetic Studies Of Hypotrichosis In District Pakpattan, Punjab, Pakistan, Amir Anees, Muhammad Abdullah, Khawar Hayyat, Muhammad Irshad, Muhammad Iqbal Usama, Muhammad Saleem Khan, Muhammad Rizwan, Muhammad Wajid
Clinical And Genetic Studies Of Hypotrichosis In District Pakpattan, Punjab, Pakistan, Amir Anees, Muhammad Abdullah, Khawar Hayyat, Muhammad Irshad, Muhammad Iqbal Usama, Muhammad Saleem Khan, Muhammad Rizwan, Muhammad Wajid
Journal of Bioresource Management
Hypotrichosis is a hereditary hair development condition that results in thin or little hair on the head and other regions of the body due to diminished or non-existent hair growth. To investigate the prevalence rate, the mechanism of inheritance, and the genetic counselling of people affected by these conditions. To determine the number of cases of familial hypotrichosis, a survey was done at various schools, colleges, hospitals, and communities to identify those affected by this ailment. A study of eleven families with consanguineous marriages found that 10.52 % of the population had hypotrichosis. The affected individuals had limited hair growth …
Dynamics And Optimal Intervention Strategies In A Shigellosis Transmission Model, Mehmet Gümüs, Shewafera Wondimagegnhu Teklu, Kemal Türk
Dynamics And Optimal Intervention Strategies In A Shigellosis Transmission Model, Mehmet Gümüs, Shewafera Wondimagegnhu Teklu, Kemal Türk
Mathematical Modelling and Numerical Simulation with Applications
This research explores how water treatment contributes to limiting the transmission of Shigellosis, an infection caused by bacteria from the Shigella genus. A mathematical framework is formulated to evaluate the influence of protective strategies and water purification on the spread of the disease. To confirm the model's biological relevance, its well-posedness is investigated. The basic reproduction number $(\mathfrak{R}_0)$, a critical indicator of disease behavior, is derived using the matrix operator method. Findings indicate that if $\mathfrak{R}_01$, the infection persists, with the endemic equilibrium exhibiting local asymptotic stability. A comprehensive cost-effectiveness analysis reveals that combining environmental protection with water treatment represents …
Seroprevalence, Risk Factors, And Diagnostic Accuracy Of Brucellosis In Upper Dir District, Khyber Pakhtunkhwa, Pakistan, Sidra Sanam, Kamran Khan
Seroprevalence, Risk Factors, And Diagnostic Accuracy Of Brucellosis In Upper Dir District, Khyber Pakhtunkhwa, Pakistan, Sidra Sanam, Kamran Khan
Journal of Bioresource Management
Brucellosis is a zoonotic disease caused by the gram-negative, intracellular bacterium Brucella. Although it is endemic in Pakistan, comprehensive data on its prevalence, risk factors, and diagnostic accuracy remain limited. Therefore, this study aimed to assess these parameters in local communities of District Dir Upper, Khyber Pakhtunkhwa, Pakistan. A total of 345 individuals suspected of brucellosis were investigated. Blood samples and structured questionnaires were collected to determine prevalence and identify associated risk factors. Additionally, 50 culture-confirmed positive cases and 50 negative controls were used to evaluate the diagnostic performance of Enzyme-Linked Immunosorbent Assay (ELISA) and Serum Plate Agglutination Test …
Statice Cut Flower Production In Utah, Kellie Webb, Melanie Stock, Benjamin Scow, Cheyenne Reid, Victoria Xiong, Ali Harrison, Lorin Harrison, Amanda Pratt, Claudia Nischwitz, Nick Volesky
Statice Cut Flower Production In Utah, Kellie Webb, Melanie Stock, Benjamin Scow, Cheyenne Reid, Victoria Xiong, Ali Harrison, Lorin Harrison, Amanda Pratt, Claudia Nischwitz, Nick Volesky
All Current Publications
Statice is a cut-and-come-again, full-sun annual with clustered, papery blooms. Plants have lower water needs and a moderate tolerance to salinity, making this crop a sustainable choice for cut flower production in Utah and other states with water and soil limitations. This fact sheet provides information on statice cultivars, site preparation, germination, transplanting and spacing, trellising, nutrient management, harvest, economics for producers, and pest and disease management.
Structural Variation Of The Right Coronary Ostium And Its Implications To Cardiac Medicine, Jessica L. Hensley, Joseph X. Anders, Timothy Robinson, William Srinivasan, Donald G. Matz, Ethan L. Snow
Structural Variation Of The Right Coronary Ostium And Its Implications To Cardiac Medicine, Jessica L. Hensley, Joseph X. Anders, Timothy Robinson, William Srinivasan, Donald G. Matz, Ethan L. Snow
The Journal of Integrative Physiology
Introduction: The high oxygen demand of the heart’s myocardium is met via perfusion through the coronary arterial system. During embryological development, the right (RCA) and left (LMCA) coronary arteries fuse onto the aortic root, creating right (RCO) and left (LCO) ostial openings respectively to join the closed circulatory system. Regardless of their widely variable branching patterns, the ability of the RCA and LMCA to perfuse the myocardium initially relies on the functional patency of the coronary ostia. The objective of this study is to examine a unique extra-ostial tissue structure found overlying an RCO and provide a literature-based discussion of …
Synthesis And Characterization Of Fe-Zn Bimetallic Nanoparticles Via Two-Step Laser Ablation And Their Antibacterial Activity, Hudhaifa M. Mohammed, Sahar Naji Rashid
Synthesis And Characterization Of Fe-Zn Bimetallic Nanoparticles Via Two-Step Laser Ablation And Their Antibacterial Activity, Hudhaifa M. Mohammed, Sahar Naji Rashid
Karbala International Journal of Modern Science
In this study, Nd: YAG laser at a wavelength of (1064 nm), energies of (300 and 400 mJ), and a pulse repetition rate of (3 Hz) was used to synthesize metallic nanoparticles from iron and zinc individually using a one-step pulsed laser ablation approach, followed by the two-step synthesis of bimetallic nanoparticles. The physical properties of the synthesized nanoparticles were then investigated using UV-visible (UV-Vis), X-ray diffraction (XRD), field-emission scanning electron microscopy (FESEM), and energy-dispersive X-ray (EDX) techniques. The results of characterization of the obtained NPs confirmed the formation of core-shell nanocomposites, as evidenced by the increased absorbance intensity of …
The Path To A Green Transition: Just And Inclusive, Nour Elden Tarek Anwr Abd Elshafy
The Path To A Green Transition: Just And Inclusive, Nour Elden Tarek Anwr Abd Elshafy
COP30
This paper explores the opportunities and challenges in achieving Just Transition in the agricultural sector, focusing on the critical areas of Climate investments in the field of training and public awareness, policy formulation, and promoting Inclusive sustainable livelihoods for the most affected groups, namely people with disabilities. It highlights the current gaps in Climate investments, the need for inclusive and effective policies, and the importance of ways to achieve just integration to reduce Poverty and unemployment rates. The paper proposes actionable solutions to align financial investments, policy frameworks, and Comprehensive practices with the Paris Agreement goals and Glasgow talks (COP26), …
Operationalising Blue Carbon: Australia's Framework As A Model For Global Nature-Based Climate Mitigation, Chelsea Golding
Operationalising Blue Carbon: Australia's Framework As A Model For Global Nature-Based Climate Mitigation, Chelsea Golding
COP30
Blue carbon ecosystems offer a critical solution to nature-based climate change mitigation. These habitats not only sequester significant amounts of carbon per hectare but also support biodiversity and increase climate resilience for coastal communities. Yet, despite the widespread recognition of these benefits in global climate discussions, most existing carbon sequestration and crediting frameworks remain focused on terrestrial ecosystems and fail to account for the greater variability in marine and costal habitats. This policy gap creates a significant barrier in the capacity for costal carbon sequestration projects to be scaled globally. Australia has addressed this implementation gap at a national level …
Design And Validation Of The Telemedicine Comfort Tool, Stephanie Amador
Design And Validation Of The Telemedicine Comfort Tool, Stephanie Amador
Doctoral Dissertations and Projects
In recent years, telemedicine has become a valuable resource to improve communication between patients and health care providers. There are many benefits to telemedicine, but they are only realized if patients and healthcare providers can establish a comforting and trusting relationship between them. Although Telemedicine has positively impacted health care, it is only successful if patients and health care providers can communicate effectively in the virtual environment. This quantitative study developed and validated the Telemedicine Comfort Tool among members of Veterans on Deck. The Telemedicine Comfort Tool is a survey instrument with the goal of measuring the comfort level of …
Characteristics And Potential Indigenous Bacteria Isolated From Shallot Farming In Brebes Regency As Diazinon Pesticide Remediation Agents, Wahyu Purbalisa, Dian Hendrayanti, Wahida Annisa Yusuf
Characteristics And Potential Indigenous Bacteria Isolated From Shallot Farming In Brebes Regency As Diazinon Pesticide Remediation Agents, Wahyu Purbalisa, Dian Hendrayanti, Wahida Annisa Yusuf
Makara Journal of Science
Diazinon is an organophosphate pesticide that is widely used by shallot farmers in Brebes Regency. Due to its toxicity, diazinon residue in agricultural lands as well as plants’ cultivation is harmful to humans and the environment. Using indigenous soil bacteria as a remediation agent is an alternative solution to overcome diazinon contamination. Firstly, this study aimed to characterize isolates from diazinon-contaminated shallot farming. The second objective was to test the diazinon tolerance of the isolates in various diazinon concentrations. Isolation was carried out using nutrient agar (NA) media containing diazinon concentrations of 5, 25, 50, and 100 ppm. The characterization …
Detection Method For Escherichia Coli Using Real-Time Polymerase Chain Reaction Targeting The Yhav Gene, Muktiningsih Nurjayadi, Anisa Fitriyanti, Royna Rahma Musie, Gusti Angieta Putri, Puan Aqila Azizah, Helzi Angelina, Grace Grace, Ananda Indah Putri Sihombing, Agus Setiawan, Jefferson Lynford Declan, Gladys Indira Putri, Dandy Akbar Juliansyah, Siti Fatimah, Ayu Berkahingrum, Irma Ratna Kartika, Fera Kurniadewi, Vira Saamia, Shyi-Tien Chen, Bassam Aboemolak, Hesham Ali El Enshasy
Detection Method For Escherichia Coli Using Real-Time Polymerase Chain Reaction Targeting The Yhav Gene, Muktiningsih Nurjayadi, Anisa Fitriyanti, Royna Rahma Musie, Gusti Angieta Putri, Puan Aqila Azizah, Helzi Angelina, Grace Grace, Ananda Indah Putri Sihombing, Agus Setiawan, Jefferson Lynford Declan, Gladys Indira Putri, Dandy Akbar Juliansyah, Siti Fatimah, Ayu Berkahingrum, Irma Ratna Kartika, Fera Kurniadewi, Vira Saamia, Shyi-Tien Chen, Bassam Aboemolak, Hesham Ali El Enshasy
Makara Journal of Science
Escherichia coli is a foodborne pathogenic bacterium that can cause diarrhea, while yhaV is a virulence-associated gene linked to the toxin–antitoxin system in E. coli. This study was aimed at evaluating the confirmation, specificity, and sensitivity of a yhaV gene primer using real-time polymerase chain reaction. The yhaV-targeting PCR successfully amplified a DNA fragment with an amplicon length of 207 bp (base pairs) under an annealing temperature optimized to a range of 54 °C to 62 °C via gradient PCR. The PCR using the primer pair produced a consistent Ct (cycle threshold) of 14.14 ± 0.05 and showed …
Employing Seca Recombinant Protein To Generate Polyclonal Antibodies For The Rapid Identification Of Phytoplasma, Arik Arubil Fatinah, Muhaimin Rifa'i, Estri Laras Arumingtyas, Sri Widyarti
Employing Seca Recombinant Protein To Generate Polyclonal Antibodies For The Rapid Identification Of Phytoplasma, Arik Arubil Fatinah, Muhaimin Rifa'i, Estri Laras Arumingtyas, Sri Widyarti
Makara Journal of Science
Phytoplasma, a plant pathogen that threatens plant health, can induce diseases such as yellow wilt, virescence, phyllody, and witches’ broom. Although early detection methods using polyclonal and monoclonal antibodies have been developed, synthetic peptides offer advantages such as higher titers, predictable antigenic characteristics and superior sensitivity and specificity. Epitope mapping plays a key role in designing peptide antigens and includes gene synthesis, transformation, expression assessment, large-scale production, and purification. A study using New Zealand male White rabbits was conducted to generate antibodies against phytoplasma. The SecA (395–470) sequence, obtained from the preprotein translocase meets the criteria of being non-homologous, nonallergic, …
Gama Melon Parfum, A Potential Melon Genotype Tolerant Of Salinity Stress, Giri Nugroho, Tatik Chikmawati, Dian Sartika, Adib Fakhruddin Yusuf, Karso Suryo Putro, Vida Rahma Latifah, Budi Setiadi Daryono, Anjar Tri Wibowo, Awik Puji Dyah Nurhayati, Karlia Meitha, Miftahudin Miftahudin
Gama Melon Parfum, A Potential Melon Genotype Tolerant Of Salinity Stress, Giri Nugroho, Tatik Chikmawati, Dian Sartika, Adib Fakhruddin Yusuf, Karso Suryo Putro, Vida Rahma Latifah, Budi Setiadi Daryono, Anjar Tri Wibowo, Awik Puji Dyah Nurhayati, Karlia Meitha, Miftahudin Miftahudin
Makara Journal of Science
Melona, Tacapa Gold, and Gama Melon Parfum are novel Melon genotypes that have been introduced for cultivation in the saline-prone coastal region of Bocor Beach, Kebumen, Central Jawa. Elevated salt concentrations in the area pose challenges, impacting yield. The potential of these new genotypes for saline land remains unexplored. This study aimed to assess the morphophysiological and molecular responses of these genotypes to salinity stress. Seeds were germinated in cocopeat and ABmix 3000 solution and treated with 220 mM NaCl until harvest. The analysis included morphology, chlorophyll content, proline accumulation, malondialdehyde levels, and gene expression were analyzed. After 1 week …
Tv Versus Video Games: Their Distinctive Effects On Adolescent Behavior And Academic Achievement, Faiaz Rahman, Kathy L. Schuh
Tv Versus Video Games: Their Distinctive Effects On Adolescent Behavior And Academic Achievement, Faiaz Rahman, Kathy L. Schuh
Mid-Western Educational Researcher
The current paper uses data collected from a publicly available, nationally representative data set to examine the effects of TV viewing and video gaming on adolescent behavioral problems and cognitive achievement. Results revealed that extended hours of TV viewing were associated with greater anxiety/depression and antisocial problems. On the other hand, extended hours of video gaming were associated with higher reading comprehension scores. Differing effects of TV viewing and gameplay by an individual’s sex was also found during the analysis. It is also important to note that the opposite effects that the two media has on outcomes, indicate that, although …
Enhancing Science Learning Outcomes With Sitaya Miniature Media: A Quantitative Study Of Elementary Education, Gilang Kripsiyadi Praramdana, Hamdan Hamdan, Agus Gunawan, Fauziah Dwi Hijriani
Enhancing Science Learning Outcomes With Sitaya Miniature Media: A Quantitative Study Of Elementary Education, Gilang Kripsiyadi Praramdana, Hamdan Hamdan, Agus Gunawan, Fauziah Dwi Hijriani
Jurnal Pendidikan Sains
This study investigates the impact of SITAYA Miniature media on the learning outcomes of grade VI students in science subjects at SDN 2 Darma. Conventional teaching methods, often less engaging for students, were identified as a significant factor contributing to low academic performance. The study employed a quantitative research approach, with a randomized sample of 43 students divided into an experimental group (24 students) and a control group (19 students). Pretest and posttest data were collected using a structured test instrument, and statistical analyses, including normality, homogeneity, t-tests, and N-Gain tests, were conducted to assess the effectiveness of SITAYA Miniature …
Chacahoula 2025, Volume 92, Alayna Pellegrin
Chacahoula 2025, Volume 92, Alayna Pellegrin
Chacahoula
The 2025 issue of Chacahoula includes December 2024 and May 2025 graduating classes, as well as stunning photography student life on the banks of Bayou Desiard. With features covering students, faculty, and staff, this year's time capsule preserves an academic year in the life of the University of Louisiana at Monroe.
Evaluation Of Robotic Swabbing And Fluorescent Sensing To Monitor The Hygiene Of Food Contact Surfaces, Siavash Mahmoudi, Clark Griscom, Pouya Sohrabipour, Yang Tian, Chaitanya Palleria, Philip G. Crandall, Dongyi Wang
Evaluation Of Robotic Swabbing And Fluorescent Sensing To Monitor The Hygiene Of Food Contact Surfaces, Siavash Mahmoudi, Clark Griscom, Pouya Sohrabipour, Yang Tian, Chaitanya Palleria, Philip G. Crandall, Dongyi Wang
Biological and Agricultural Engineering Faculty Publications and Presentations
Effective environmental monitoring is critical for preventing microbial and allergenic cross-contamination. However, manual swabbing methods, commonly used to verify hygienic conditions, are prone to inconsistent results because of variability in pressure, coverage, and techniques. Two novel solutions will be explored to address these challenges: a robotic swabbing system with tactile sensing control, and a fluorescence/absorbance spectrometer for non-contact, protein-based residue detection. The robotic system was evaluated against trained and untrained humans, measuring water pickup, surface coverage, and pressure consistency. Concurrently, the fluorescence system analyzed model poultry protein soil to correlate spectral patterns with contamination levels. The robotic system demonstrated statistically …
Nontarget Effects Of Abamectin On Cysts And Three Larval Stages Of Marine Microcrustacean Artemia Franciscana, Hashem Khandan Barani, Sahel Pakzad Toochaei, Narjes Sanchooli
Nontarget Effects Of Abamectin On Cysts And Three Larval Stages Of Marine Microcrustacean Artemia Franciscana, Hashem Khandan Barani, Sahel Pakzad Toochaei, Narjes Sanchooli
Turkish Journal of Zoology
Abamectin (ABM) is a widely used pesticide in agriculture and aquaculture worldwide. However, limited information is available on the toxic impacts of ABM on nontarget marine organisms, necessitating further research to investigate the toxic impacts of ABM on marine life. Hence, this study examined the acute (0.5–2.5 μg/L for 24 and 48 h) and chronic (0.125–0.5 μg/L for 7 days) toxicity of ABM on the marine microcrustacean Artemia franciscana under different incubation conditions and at different stages of development. To simulate a more realistic environmental scenario, some of the exposure tests were conducted in the presence of food or with …
Cytotoxic And Genotoxic Effects Of Minor Temperature Changes And Zinc Oxide Nanoparticles Synthesized Using Plantago Major On Oncorhynchus Mykiss Gonadal Cells, Semra Çi̇çek, Sevda Işik
Cytotoxic And Genotoxic Effects Of Minor Temperature Changes And Zinc Oxide Nanoparticles Synthesized Using Plantago Major On Oncorhynchus Mykiss Gonadal Cells, Semra Çi̇çek, Sevda Işik
Turkish Journal of Zoology
Temperature changes and nanoparticle exposure have emerged as critical environmental stressors with potential cytotoxic and genotoxic effects on aquatic species. This study investigates the impact of zinc oxide nanoparticles (ZnO NPs), synthesized using Plantago major leaves, on Oncorhynchus mykiss gonadal (RTG-2) cells under different temperature conditions (23 °C, 24 °C, and 25 °C). RTG-2 cells were exposed to hexagonal ZnO NPs (≤100 nm) at concentrations of 6.25, 12.5, 25, 50, 75, and 100 μg/mL for 24 and 48 h. Cytotoxicity was assessed using the sulforhodamine B assay, and genotoxicity was evaluated via the comet assay. Results demonstrated that both temperature …
Rna/Dna-Binding Protein Tdp43 Regulates Dna Mismatch Repair Genes With Implications For Genome Stability, Vincent E Provasek, Albino Bacolla, Suganya Rangaswamy, Manohar Kodavati, Joy Mitra, Issa O Yusuf, Vikas H Malojirao, Velmarini Vasquez, Gavin W Britz, Guo-Min Li, Zuoshang Xu, Sankar Mitra, Ralph M Garruto, John A Tainer, Muralidhar L Hegde
Rna/Dna-Binding Protein Tdp43 Regulates Dna Mismatch Repair Genes With Implications For Genome Stability, Vincent E Provasek, Albino Bacolla, Suganya Rangaswamy, Manohar Kodavati, Joy Mitra, Issa O Yusuf, Vikas H Malojirao, Velmarini Vasquez, Gavin W Britz, Guo-Min Li, Zuoshang Xu, Sankar Mitra, Ralph M Garruto, John A Tainer, Muralidhar L Hegde
Faculty, Staff and Student Publications
TDP43 is an RNA/DNA-binding protein increasingly recognized for its role in neurodegenerative conditions, including amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). As characterized by its aberrant nuclear export and cytoplasmic aggregation, TDP43 proteinopathy is a hallmark feature in over 95% of ALS/FTD cases, leading to detrimental cytosolic aggregates and a reduction in nuclear functionality in neurons. Building on our prior work linking TDP43 proteinopathy to the accumulation of DNA double-strand breaks (DSBs) in neurons, the present investigation uncovers a novel regulatory relationship between TDP43 and DNA mismatch repair (MMR) gene expression. Here, we show that TDP43 depletion or overexpression …
Functional Role Of Cysteine Residues In Vtlr Of Agrobacterium Tumefaciens, Suravi Islam
Functional Role Of Cysteine Residues In Vtlr Of Agrobacterium Tumefaciens, Suravi Islam
Theses and Dissertations
Agrobacterium tumefaciens is a soil-dwelling plant pathogen known for transferring DNA to host plants through a type IV secretion system. Central to its virulence and cellular regulation is the LysR-type transcriptional regulator, VtlR, which controls processes including biofilm formation, motility, tumorigensis, and small RNA expression. Although VtlR’s regulatory role is established, the specific functions of its conserved cysteine residues have only recently begun to be explored. This study investigates VtlR’s four cysteines (C144, C236, C244, and C273) through site-directed mutagenesis and a combination of phenotypic assays and transcriptional profiling. Phenotypic assays confirmed that C144 and C273 are important for proper …
Interleukin-6 (Il-6) Mediated Pathways In Nonalcoholic Fatty Liver Disease (Nafld) And Its Implications On Magnesium And Zinc Mineral Deficiencies: A Scoping Review, Marianne Koleng, Leann Nguyen
Interleukin-6 (Il-6) Mediated Pathways In Nonalcoholic Fatty Liver Disease (Nafld) And Its Implications On Magnesium And Zinc Mineral Deficiencies: A Scoping Review, Marianne Koleng, Leann Nguyen
Department of Nutrition Student Projects
The prevalence and incidence of Non-Alcoholic Fatty Liver Disease (NAFLD) is rising. In the United States alone, the prevalence of NAFLD is currently 38% and there has been a 50% increase over the last three decades. The present study investigated the role of ultratrace mineral deficiencies, specifically magnesium (Mg) and zinc (Zn), and if so, what nutritional interventions could be made to ameliorate or protect against the development of NAFLD. The role of trace and ultratrace minerals in pro-inflammatory cytokine pathways contributing to NAFLD, specifically Interleukin-6 (IL-6), were investigated. IL-6 is produced by hepatocytes and plays a crucial role in …
Symbiotic Aspergillus Terreus Alleviates Drought And Waterlogged Stress In Zea Mays L., Hazrat Ali, Fayaz Asad, Naveen Dilawar, Vasila Sharipova, Imtiaz Ahmad, Abida Zeb
Symbiotic Aspergillus Terreus Alleviates Drought And Waterlogged Stress In Zea Mays L., Hazrat Ali, Fayaz Asad, Naveen Dilawar, Vasila Sharipova, Imtiaz Ahmad, Abida Zeb
Turkish Journal of Botany
The interaction concerning rhizospheric fungi and plants is essential for enhancing plant resilience in the face of abiotic stress conditions. This study investigated the effects of the rhizospheric fungus Aspergillus terreus on maize (Zea mays L.) growth under both drought and waterlogging conditions. Our findings confirmed that inoculation with A. terreus mitigated the adverse effects of drought and waterlogging conditions on maize growth. Plants treated with Aspergillus terreus (A. terreus) exhibited enhanced root and shoot length, fresh and dry weight as well as enhanced chlorophyll content in plants compared to control, under stress-related conditions. Compared to the control group, A. …