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From Genes To Streams: Mitochondrial Approaches To Population Monitoring Of The Santa Ana Sucker Catostomus Santaanae, Mariana Antonio Aug 2026

From Genes To Streams: Mitochondrial Approaches To Population Monitoring Of The Santa Ana Sucker Catostomus Santaanae, Mariana Antonio

Electronic Theses, Projects, and Dissertations

This study was developed to use aquatic environmental DNA (eDNA) as a tool to detect the presence of the Santa Ana sucker (Catostomus santaanae), a federally threatened fish species, across the Santa Ana River in Southern California. Environmental DNA is any genetic material that an organism sheds into the environment. We can get an idea of what aquatic species are present when sampling water at a given site. We sequenced the complete mitochondrial genome (mitogenome) of the Santa Ana sucker and designed custom DNA primers to improve eDNA detection accuracy. These primers were selected based on high genetic …


Integrated Soil-Water Processes And Irrigation Management For Agricultural Water Optimization In Semi-Arid Regions, Tejinder Singh Aug 2026

Integrated Soil-Water Processes And Irrigation Management For Agricultural Water Optimization In Semi-Arid Regions, Tejinder Singh

All Graduate Theses and Dissertations, Fall 2023 to Present

Water is one of the most important resources for agriculture in the western United States. In semi-arid regions, farmers depend on irrigation to grow crops, but water supplies are becoming less reliable because of drought, declining snowpack, groundwater depletion, and increasing competition for limited water. Improving agricultural water use is therefore not simply about applying less water. It also requires understanding where water goes after it enters a field, how long it remains available to crops, and how farmers can use that information to make better irrigation decisions. This dissertation follows water through irrigated agricultural systems to better understand how …


Drought And Diet Breadth: Does Insect Specialization Influence Herbivory When Plants Are Under Stress?, Jakob Palmer Aug 2026

Drought And Diet Breadth: Does Insect Specialization Influence Herbivory When Plants Are Under Stress?, Jakob Palmer

All Graduate Theses and Dissertations, Fall 2023 to Present

Climate change will alter precipitation worldwide, causing more frequent and severe drought events worldwide. Drought affects many ecological processes, including plant-herbivore interactions, which are critical to biodiversity and ecosystem health. A variety of plant characteristics can influence herbivory – such as how hairy, nutritious, or toxic a leaf is – and drought has been shown to alter many of these traits. Unique characteristics of herbivore species are also important: herbivores can either feed on a variety of plants (generalists) or a group of closely related plants (specialists). Specialists can cope with traits of the plants they eat, while generalists are …


Microbes In Pollination Systems: Effects On Pollinator Health, Floral Traits, Nectar Robbing, And Plant Fitness, Valerie Nicole Martin Aug 2026

Microbes In Pollination Systems: Effects On Pollinator Health, Floral Traits, Nectar Robbing, And Plant Fitness, Valerie Nicole Martin

All Graduate Theses and Dissertations, Fall 2023 to Present

Flowers produce nectar to attract pollinators, but nectar is also a habitat for yeasts and bacteria that arrive via pollinators, robbers, and other visitors. These microbes consume nectar sugars and release byproducts that change the smell and taste of nectar. Whether these changes affect how pollinators find and choose flowers, and whether they ultimately affect how many seeds a plant produces, is not well understood. In this dissertation, I studied how a common nectar yeast and a plant-associated bacterium affect the scent of wildflowers, the behavior of bumble bees and hummingbirds, and plant reproduction in montane meadows in central Colorado. …


Seizure Susceptibility And Morphological Analysis Of A Missense Mutation In Hcfc1a Protein, Jose Manuel Fabela Aug 2026

Seizure Susceptibility And Morphological Analysis Of A Missense Mutation In Hcfc1a Protein, Jose Manuel Fabela

Open Access Theses & Dissertations

Missense mutations in the HCFC1 gene cause methymalonic acidemia with homocystinuria cblX type (MIM#309541). cblX is an X-linked disorder that is associated with multiple phenotypes such as metabolic defects, failure to thrive, intractable epilepsy, facial dysmorphia, movement disorders, and intellectual disability. The mechanisms underlying these phenotypes are not completely understood. Here we used a zebrafish germline mutant allele (hcfc1aco64/co64) to study seizure susceptibility and adult brain structural malformations. Previous studies have established neural progenitor cell defects and hypoactive mechanistic target of rapamycin (mTOR) in this allele. Given the known role of mTOR in seizure phenotypes, we hypothesized that missense mutation …


Optimizing Urban Forestry Through Green Infrastructure: Tree Health And Water Retention In Ciudad Juarez Rain Gardens, Tomas Rogelio Lizarraga Ruiz Aug 2026

Optimizing Urban Forestry Through Green Infrastructure: Tree Health And Water Retention In Ciudad Juarez Rain Gardens, Tomas Rogelio Lizarraga Ruiz

Open Access Theses & Dissertations

Urban green spaces (UGS) in arid regions face significant challenges due to water scarcity and extreme thermal demand. This study evaluates the efficacy of Nature Based Solutions (NBS), rain gardens and median strips, in enhancing the physiological resilience of urban trees in Ciudad Juárez, Mexico. Using a comparative framework, the research monitored a native evergreen (Dermatophyllum secundiflorum) and an introduced deciduous species (Morus sp.) across six planting treatments. Statistical analysis reveals that green infrastructure (GI) acts as a critical physiological accelerant. Trees situated within rain gardens and medians significantly outperformed those in restricted pot and sidewalk settings, maintaining higher stomatal …


An Analysis Of Mating Strategies In Rotifers Inhabiting Temporary Habitats, Ariel Bollinger Aug 2026

An Analysis Of Mating Strategies In Rotifers Inhabiting Temporary Habitats, Ariel Bollinger

Open Access Theses & Dissertations

Reproduction is critical for organisms inhabiting ephemeral aquatic habitats where desiccation risk favors rapid and successful mating. Monogonont rotifers rely on sexual reproduction to produce diapausing eggs that withstand harsh conditions. I investigated how mating behaviors vary among populations of five rotifer species based on the hydroperiod of their habitat: Epiphanes brachionus and E. chihuahuaensis were sampled from habitats with short (SH) and long (LH) hydroperiods; Euchlanis dilatata was collected from a short (SH) and a permanent habitat; and Plationus patulus and Sinantherina socialis were collected from a long (LH) and a permanent (Perm) habitat. I hypothesized that SH populations …


Trace Element Concentrations In Tissues And Otoliths Of Fishes From Artificial Reefs In The Northern Gulf Of Mexico: The Role Of Size, Age, And Trophic Position, Blaine Savoie Aug 2026

Trace Element Concentrations In Tissues And Otoliths Of Fishes From Artificial Reefs In The Northern Gulf Of Mexico: The Role Of Size, Age, And Trophic Position, Blaine Savoie

LSU New Orleans Theses and Dissertations

Nearshore artificial reefs and fishes relying on them to forage and refuge can be susceptible to trace element (TE) pollutants from anthropogenic sources. Although some TE are essential, higher concentrations can cause detrimental effects and concern regarding consumption. This study assesses TE accumulation in nearshore reef fishes in the northern Gulf of Mexico, a region influenced by coastal riverine discharge, where fishing has socioeconomic importance. Five fish species from six eco-reefs were collected and analyzed for TE. An inductively coupled plasma mass spectrometer was used to analyze the TE concentrations of muscle, liver, and otoliths. TE concentrations of tissues and …


Menopausal Symptoms, Psychosocial Factors, And Physical Activity Among Physically Active Midlife Females, Ella Onsoriasl Aug 2026

Menopausal Symptoms, Psychosocial Factors, And Physical Activity Among Physically Active Midlife Females, Ella Onsoriasl

Exercise Science Theses & Dissertations

Menopausal symptoms are common during midlife and may be associated with females' physical activity (PA) participation, yet how these symptoms relate to PA levels, perceived confidence and enjoyment, and psychosocial resources such as social support (SS), exercise identity, and quality of life (QOL) remains unclear. The purpose of this study was to examine how menopausal symptom severity relates to PA levels, perceived confidence and enjoyment during PA, and psychosocial factors, including SS, exercise identity, and QOL. This cross-sectional study surveyed 151 midlife females aged 40 to 60 who reported engaging in intentional PA at least once per week. Participants completed …


Effects Of Beverage Color During Mouth Rinsing On Measures Of Muscular Endurance, Muscular Strength, And Exercise Perceptions During Isometric Handgrip Testing, Sydney Andrews Aug 2026

Effects Of Beverage Color During Mouth Rinsing On Measures Of Muscular Endurance, Muscular Strength, And Exercise Perceptions During Isometric Handgrip Testing, Sydney Andrews

Exercise Science Theses & Dissertations

Previous research has shown that color can influence psychological responses, physiological responses, and exercise performance. These effects have been previously attributed to learned associations or expectations which may influence exercise-related outcomes. This study aimed to investigate whether beverage color influences muscular performance during intermittent isometric handgrip exercise. This randomized, single-blind, deceptive crossover study consisted of 34 physically active adults (21.9±3.1 years) who completed a handgrip endurance test under three conditions: red beverage rinse, clear beverage rinse, and no-rinse. Participants were informed that the study would examine the effects of mouth rinsing two commercially available carbohydrate containing sport drinks on muscular …


Evaluating Fish Assemblages At An Early-Stage Living Shoreline Project In Lake Pontchartrain, Collin Glaser Aug 2026

Evaluating Fish Assemblages At An Early-Stage Living Shoreline Project In Lake Pontchartrain, Collin Glaser

LSU New Orleans Theses and Dissertations

Living shorelines are an increasingly popular restoration approach for reducing erosion while improving habitat quality. The Bucktown Living Shoreline Project is a large-scale restoration effort along the southern shore of Lake Pontchartrain aimed at enhancing coastal resilience and restoring historic marsh habitat. This thesis evaluated early fish assemblage response to the Bucktown Living Shoreline Project over one year and placed those assemblages within the broader historical context of Lake Pontchartrain. Monthly sampling was conducted at the living shoreline and an adjacent riprap control site using seines, cast nets, and minnow traps. Fish assemblage structure was not significantly different between sites. …


Forest Cover Effects On Canopy Interception Loss, Evapotranspiration, And Soil Infiltration On Stormwater Detention Basins, Alexandria N. C. Perez Aug 2026

Forest Cover Effects On Canopy Interception Loss, Evapotranspiration, And Soil Infiltration On Stormwater Detention Basins, Alexandria N. C. Perez

Electronic Theses and Dissertations

Developing cities and increased impervious surfaces has resulted in more surface runoff and severe flooding. Harris County, Texas has experienced major flooding events, such as Hurricane Harvey in 2017 which was estimated to be a 9,000-year flood event. The Harris County Flood Control District (HCFCD) is dedicated to creating, maintaining, and finding new ways to reduce flooding damage such as the use of detention basins as green infrastructure. These basins are either populated by grass or are planted with various tree species. This study will identify the planted trees’ canopy interception loss, reference evapotranspiration, and enhanced soil infiltration effects within …


Harnessing Kenyan Aquaculture Through Social Media: Impacts, Challenges And Strategic Directions, Domitila Kyule, Jonathan Munguti, Mavindu Muthoka, Esther Magondu, Menaga Meenakshisundaram, Chrysantus M. Tanga, Kevin Obiero, Cecilia Githukia Aug 2026

Harnessing Kenyan Aquaculture Through Social Media: Impacts, Challenges And Strategic Directions, Domitila Kyule, Jonathan Munguti, Mavindu Muthoka, Esther Magondu, Menaga Meenakshisundaram, Chrysantus M. Tanga, Kevin Obiero, Cecilia Githukia

All Peer-Reviewed Publications

Aquaculture is increasingly important for food, nutrition and livelihoods in Kenya, but the sector still faces persistent constraints in extension delivery, farmer learning, input access, market coordination and information reliability. This review examines how social media and related digital communication tools are shaping aquaculture development in Kenya, with attention to impacts, challenges, governance needs and strategic directions. A systematic literature review of studies and institutional sources published between 2015 and 2025 was conducted. The search identified 388 records, of which 45 sources were included in the final synthesis after screening, eligibility assessment and quality appraisal. Because the evidence was heterogeneous, …


Experimental Results With Various Herbicides In Established Alfalfa, Taib Saric, Refik Numic Jul 2026

Experimental Results With Various Herbicides In Established Alfalfa, Taib Saric, Refik Numic

IGC Proceedings (1977-2023)

5-year experiments with herbicides were run in the mixture of alfalfa, red clover, timothy grass, and Italian ryegrass. On untreated plots, in the 5-year average 72 weed plants were found per 1 m2 , belonging to 66 weed species. Among them most numerous were: Stellaria media, Veronica agrestis, Poa annua, Lamium purpureum,Taraxacum officinale, and Capsella bursa pastoris. The most efficient herbicide was Etazin, killing 88 % of weeds. None of the applied herbicides controlled perennial weeds. Etazin was tolerated by alfalfa, but was toxic to the other three crops. Kerb, Kerb-Mix, and Multi­tok were toxic to sown grasses, while …


Recommended Values For Dimensioning And Designing Sprinkler Irrigation In Industrialized Forage Plant Produc­Tion In The Gdr, Klaus Schwarz, Dieter Roth Jul 2026

Recommended Values For Dimensioning And Designing Sprinkler Irrigation In Industrialized Forage Plant Produc­Tion In The Gdr, Klaus Schwarz, Dieter Roth

IGC Proceedings (1977-2023)

With the transition to industrialized crop and animal production in the GDR, sprinkler irrigation of field forage plants and grassland, above all with sewage water and clean water +slurry mixtures, is becoming more and more important. It gets increasingly concentrated on field grass and grass/clover stands. The supple­mental water requirement is 210 to 270 mm/ year in case of clean-water sprinkling and up to 400 mm/year if sewage water is used. The hydro-modulus of the irrigation plants is be­tween 1.2 and 1.6 m3 /h/ha, depending on the simultaneity factor. In order to attain the required water quantity per unit of …


Thermoclock:A Novel Automated Temperature Regulation Device That Can Model Circadian Entrainment And Disruption In 2d And 3d In Vitro Models, Kaiyin Kelly Zhang, Cathryn A. Cutia, Chanelle A. Moise, Bhavna Kalyanaraman, Kaitlyn Chhe, Jiexin Wang, Michelle E. Farkas, Ilia N. Karatsoreos, Mary Harrington, Meghan E. Huber, Cathal J. Kearney Jul 2026

Thermoclock:A Novel Automated Temperature Regulation Device That Can Model Circadian Entrainment And Disruption In 2d And 3d In Vitro Models, Kaiyin Kelly Zhang, Cathryn A. Cutia, Chanelle A. Moise, Bhavna Kalyanaraman, Kaitlyn Chhe, Jiexin Wang, Michelle E. Farkas, Ilia N. Karatsoreos, Mary Harrington, Meghan E. Huber, Cathal J. Kearney

Biological Sciences: Faculty Publications

Circadian rhythms are critical for maintaining homeostasis and regulating physiological functions, and consequentially impact disease progression; yet, they remain largely overlooked in in vitro models used in preclinical research. One major barrier to rigorously testing the role of circadian rhythms in these models is the lack of accessible tools that seamlessly integrate into standard culture setups and are capable of sustainably delivering time cues to cells and tissues in long term experiments. Here, we present the ThermoClock, a low-cost, Arduino-based automated temperature control system capable of delivering independent temperature programs to multiple cultures simultaneously. Using circadian reporter U2OS cell lines …


Garambullo (Myrtillocactus Geometrizans) Reduces Cell Viability Via Apoptosis And Inhibits Colony Formation In Ht-29 And Sw480 Colon Cancer Cell Lines., López-Alvarez Guadalupe Soledad, Gutierrez-Silerio Gloria Yareli, García-Solís Pablo, Morales-Bárcenas Rocio, Sánchez-Pérez Yesennia, Hernández-Puga Ana Gabriela, Vergara-Castañeda Haydé Azeneth Jul 2026

Garambullo (Myrtillocactus Geometrizans) Reduces Cell Viability Via Apoptosis And Inhibits Colony Formation In Ht-29 And Sw480 Colon Cancer Cell Lines., López-Alvarez Guadalupe Soledad, Gutierrez-Silerio Gloria Yareli, García-Solís Pablo, Morales-Bárcenas Rocio, Sánchez-Pérez Yesennia, Hernández-Puga Ana Gabriela, Vergara-Castañeda Haydé Azeneth

Journal of Food and Drug Analysis

Garambullo (Myrtillocactus geometrizans) is a Mexican fruit rich in bioactive phytochemicals. Its fermented extract (FEG), obtained after in vitro gastrointestinal digestion and fermentation, was evaluated in HT-29 and SW480 colorectal cancer cells. FEG reduced cell viability (IC50: 2985 and 3200 μg/mL, respectively) and induced apoptosis and necrosis. In 3D assays, FEG inhibited colony formation, with complete suppression in HT-29 cells. Molecular docking further suggested that butyric acid identified in FEG interacts with Caspase-3 and RIPK1. These findings support the antiproliferative and pro-death effects of FEG in colorectal cancer models.


Gut Microbiota-Mediated Modulation Of Circadian Rhythms: Physiological And Molecular Impacts Of Dietary Composition And Feeding Time, Yen-Chun Koh, Chun-Che Chang, Min-Hsiung Pan Jul 2026

Gut Microbiota-Mediated Modulation Of Circadian Rhythms: Physiological And Molecular Impacts Of Dietary Composition And Feeding Time, Yen-Chun Koh, Chun-Che Chang, Min-Hsiung Pan

Journal of Food and Drug Analysis

Circadian rhythms regulate fundamental aspects of physiology and behavior. The disruption of circadian rhythms has been associated with metabolic disorders, cardiovascular disease, obesity, and impaired immune function. Feeding behavior and gut microbiota represent two essential nodes within this regulatory network, as both are highly responsive to environmental cues such as diet composition and sleep-wake cycles. Research has shown that high-fat diets, excessive fructose, and chronic alcohol consumption perturb peripheral circadian rhythms and impair metabolic pathways, contributing to obesity and insulin resistance. Conversely, dietary interventions such as time-restricted feeding and macronutrient-specific entrainment can restore rhythmic gene expression and prevent metabolic pathologies. …


Governance, Culture, And Law: The Expanding Scope Of Environmental Science And Sustainable Development Research, Ahyaudin Sodri, Herdis Herdiansyah Jul 2026

Governance, Culture, And Law: The Expanding Scope Of Environmental Science And Sustainable Development Research, Ahyaudin Sodri, Herdis Herdiansyah

Journal of Environmental Science and Sustainable Development

Environmental science emerged as a discipline based on physical measurements, such as soil pollutant levels (Ailijiang et al., 2022), ecological restoration (Yue et al., 2026), city surface temperature (Shafizadeh-Moghadam et al., 2025), and microbial density in air samples (Hayleeyesus & Manaye, 2014). However, its objects of study increasingly extend beyond the confines of the laboratory. Questions that were once sufficiently answered with measurement data now require answers through draft laws, Constitutional Court decisions, financial reports of mining companies, and even traditional ritual discourses governing community access to forests. In other words, environmental …


Oncogenic E-Cadherin-Dependent Metastasis And Potential Therapeutic Interventions, Madison K. Duenas, Anjela Anand, Paige Cano, Ralph Boccia, Mary Alpaugh Jul 2026

Oncogenic E-Cadherin-Dependent Metastasis And Potential Therapeutic Interventions, Madison K. Duenas, Anjela Anand, Paige Cano, Ralph Boccia, Mary Alpaugh

STEM Student Research Symposium Posters

Oncogenic E-cadherin-dependent metastasis is a paradoxical mechanism of cancer progression whereby E-cadherin, a cell-to-cell adhesion protein that normally suppresses tumor invasion, instead promotes the formation of cohesive cell tumor clusters that collectively invade surrounding tissues and metastasize. This phenomenon has been extensively studied using inflammatory breast cancer (IBC) models, including the MARY-X xenograft and SUM149 cell line, while comparisons with other breast cancer models such as MDA-MB-231 continue to provide insight into E-cadherin-independent metastatic mechanisms. This project reviews recent literature investigating the molecular mechanisms behind it and evaluates emerging therapeutic strategies targeting E-cadherin-associated cellular pathways. Laboratory techniques, including spheroid generation …


From Anaerobic Digesters To The Human Gut: Ecological Responses Of Microbiomes To Environmental And Biological Perturbations, Maliea Annholden Nipko Jul 2026

From Anaerobic Digesters To The Human Gut: Ecological Responses Of Microbiomes To Environmental And Biological Perturbations, Maliea Annholden Nipko

Theses and Dissertations

Microbial communities regulate essential ecological and metabolic processes across both engineered and host-associated ecosystems. Although these systems differ substantially in composition and function, they are governed by common ecological principles including community succession, resilience, species interactions, and responses to environmental and biological perturbations. This dissertation investigates how perturbations influence microbiome structure, function, and stability through three complementary studies spanning anaerobic digestion and the human gut microbiome. First, the effects of an extremophilic biological pretreatment using Caldicellulosiruptor bescii were evaluated in industrial-scale anaerobic digesters treating dairy manure. High- temperature pretreatment substantially altered microbial community composition, with bacterial populations remaining metabolically active …


Bridging The Gap In Science Research: Preparing Students For College- Level Research In The Sciences, Victoria Helwig, Yaoguang Li Jul 2026

Bridging The Gap In Science Research: Preparing Students For College- Level Research In The Sciences, Victoria Helwig, Yaoguang Li

UConn Library Presentations

Many first-year students enter college unprepared to fully engage with scientific literature and academic library resources. Drawing on experiences from academic librarians working with STEM students, this session explores common gaps in science research skills, including navigating databases, identifying scholarly sources, and understanding the structure of scientific literature. Participants will be introduced to practical strategies and classroom-ready activities to better prepare students for college-level research. Discussion questions: What science research skills do students struggle with most when entering college? How can high school instruction better support these skills? Where can librarians and educators collaborate to support student success?


Analysis Of The Atypical Temperature Dependence And Conformational Changes During Turnover Of A Lactobacillus Chlorogenic Acid Esterase, Nathaniel Carl, Yianni Tsigaris, Dan Ji, Nora K. Anpree, Kellie K. Omori, Cedric P. Owens Jul 2026

Analysis Of The Atypical Temperature Dependence And Conformational Changes During Turnover Of A Lactobacillus Chlorogenic Acid Esterase, Nathaniel Carl, Yianni Tsigaris, Dan Ji, Nora K. Anpree, Kellie K. Omori, Cedric P. Owens

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Many bacterial chlorogenic acid esterases (ChlEs) exhibit atypical temperature behavior, featuring activities that barely change with temperature and activity maxima that fall below the thermal denaturation point. This work focuses on a ChlE from Lactobacillus helveticus (Lh–ChlE), which has a flat temperature dependence. First, it was determined that conformational changes during Lh–ChlE turnover are not rate-limiting and that the overall rate depends on the chemical step at all temperatures. Next, Lh–ChlE’s temperature dependence was investigated using a conformational equilibrium model that assumes the existence of a temperature-dependent equilibrium between an active and an inactive conformation and an activation heat capacity …


Stability Of The Apogee Galvanic Cell Sensors In The Utah State University Biological Oxygen Demand System, Tia Lawrence, Carson Richards, Bruce Bugbee Jul 2026

Stability Of The Apogee Galvanic Cell Sensors In The Utah State University Biological Oxygen Demand System, Tia Lawrence, Carson Richards, Bruce Bugbee

Techniques and Instruments

The Utah State University Crop Physiology Laboratory has developed a six-chamber biological oxygen demand (BOD) system to quantify respiration (Kusuma & Bugbee, 2020). Like all instruments, accuracy depends on the ability to get a zero in empty containers. Here we report the signal output and zero stability in empty containers over a five-day interval.


Uv Damage Mapping Reveals Different Impacts Of Xpd Mutations On Global Genomic And Transcription-Coupled Dna Repair, Allyson Hoag Jul 2026

Uv Damage Mapping Reveals Different Impacts Of Xpd Mutations On Global Genomic And Transcription-Coupled Dna Repair, Allyson Hoag

Biomedical Sciences ETDs

Nucleotide Excision Repair (NER) is a fundamental cellular process that removes bulky and helical distorting lesions from the genome through the separate responses of its two subpathways – global genomic (GG-NER) and transcription coupled (TC-NER) DNA repair. The pathways converge with the recruitment of the TFIIH complex, a key step that is mediated by the translocase and helicase activities of  its XPD subunit. Germline mutations in XPD result in the clinical disorders, Xeroderma Pigmentosum (XP) and XP combined with Cockayne Syndrome (XP/CS). The goal of this thesis was to understand if XPD XP and XP/CS mutations differentially impact GG-NER and …


Mapping And Modeling Threat-Evoked Brain States After Early Life Adversity, Taylor W. Uselman Jul 2026

Mapping And Modeling Threat-Evoked Brain States After Early Life Adversity, Taylor W. Uselman

Biomedical Sciences ETDs

Early life adversity (ELA) increases lifelong neuropsychiatric vulnerability. Yet how ELA reorganizes brain-wide activity and circuit coordination across later experience remains unclear. This dissertation tests the hypothesis that ELA alters adult brain-wide activity and responses to threat through disrupted coordination among neural systems that regulate emotional experience, including prefrontal-limbic and monoaminergic systems. Longitudinal manganese-enhanced MRI of adult mice exposed to standard or fragmented early care, combined with computational processing and statistical modeling, quantified brain states before, during, and long after innate predator threat. These studies established that acute threat evokes large-scale, distributed brain activity that evolves over time. ELA potentiates …


Toxic Relationships: Characterization Of A Putative Virally Encoded Toxin In The Thermophilic Archaeal Fusellovirus Ssv1, Jonathan C. Abshier, Patrizia L. Alpapara, Guasåli Tomokane, Kenneth M. Stedman Jul 2026

Toxic Relationships: Characterization Of A Putative Virally Encoded Toxin In The Thermophilic Archaeal Fusellovirus Ssv1, Jonathan C. Abshier, Patrizia L. Alpapara, Guasåli Tomokane, Kenneth M. Stedman

Center for Life in Extreme Environments Publications

Mechanisms for the maintenance of chronic viruses are poorly understood, particularly for archaeal viruses. Here, we identify the product of Sulfolobus spindle-shaped virus 1 (SSV1) ORF a291 as a putative virally encoded toxin required for growth inhibition but dispensable for viral replication and virion production. Viruses lacking ORF a291 replicated their genomes and formed morphologically normal spindle-shaped particles, yet failed to inhibit the growth of uninfected Saccharolobus solfataricus. Substitution of residues at a predicted N-terminal signal peptide cleavage site abolished growth suppression without affecting replication, suggesting that secretion is essential for toxin function. Despite primary sequence divergence among fusellovirus …


Effect Of Cultivation Methods On Volatile Components In Morchella Esculenta, Liu Ni, Jiang Bo, Li Lang Jul 2026

Effect Of Cultivation Methods On Volatile Components In Morchella Esculenta, Liu Ni, Jiang Bo, Li Lang

Food and Machinery

[Objective] To comprehensively reveal the flavor and quality characteristics, and differences of Morchella esculenta under different cultivation methods, and systematically evaluate their volatile aroma components. [Methods] Qualitative and quantitative analyses are conducted on volatile aroma components in M. esculenta cultivated in greenhouses, under forests, and in caves in the vicinity of FAST by headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) techniques. The contribution of different components to the overall flavor is assessed by the relative odor activity value (ROAV) method. Based on this, principal component analysis and cluster analysis are conducted, achieving a comprehensive evaluation of the flavor …


Biomechanical And Talar Cartilage Responses To Running In Individuals With Chronic Ankle Instability, Minsub Oh Jul 2026

Biomechanical And Talar Cartilage Responses To Running In Individuals With Chronic Ankle Instability, Minsub Oh

Theses and Dissertations

Context: Chronic ankle instability (CAI) has shown altered movement patterns and joint loading, which increase the risk of posttraumatic ankle osteoarthritis. Abnormal vertical ground reaction force (vGRF) during running may contribute to talar cartilage degeneration; however, the acute biomechanical and cartilage responses to running in individuals with CAI remain unclear. Purpose: The purpose of this study was to compare running biomechanics and changes in talar cartilage thickness and T2 relaxation times before and after running between individuals with CAI and healthy controls. Methods: Twenty individuals with CAI and 20 healthy controls completed running biomechanics and magnetic resonance imaging (MRI) sessions. …


Irrigation Water Conservation And Accelerated Composting: An Exploration Of Environmentally Conscious Practices For Ornamental Horticulture, William James Carson Dunford Jul 2026

Irrigation Water Conservation And Accelerated Composting: An Exploration Of Environmentally Conscious Practices For Ornamental Horticulture, William James Carson Dunford

LSU Master's Theses

Best management practices for ornamental horticulture include proper management of irrigation water resources and substrate components. The first study investigated the effects of wind speed, wind direction, water pressure, and spray stake height on irrigation efficiency in nursery containers. Results indicated that lower spray stake height positions and lower wind speeds significantly improved the irrigation water volume captured within nursery containers by as much as 18% to 39%. Orienting spray stakes against prevailing winds further enhanced water retention, particularly at 0 and 1 inch heights. In contrast, higher stake elevations led to increased irrigation water loss by as much as …