Digital Commons Network^™

Exposure To Pm2.5 And Aging., Daniel Chris Gomes

Electronic Theses and Dissertations

Inhalation of fine airborne particulate matter (PM_2.5) is an environmental risk factor for numerous health disorders, including cardiovascular diseases (CVD). While several studies have shown that PM_2.5 exposure can induce oxidative stress, DNA damage, and inflammation, the underlying mechanisms that translate these effects into systemic pathology remain poorly understood. Some recent studies have shown that PM_2.5 exposure can lead to telomere shortening in leukocytes, but the generality of this outcome and contribution to endothelial dysfunction has not yet been fully explored. We hypothesized that PM_2.5inhalation accelerates cellular aging and senescence in both circulating peripheral …

Further Characterization Of The Fungal Effector Mvlg-01732 In Planta And Heterologous Expression Of Fungal Effectors In Different Smut Fungi., Joseph Paul Ham

Electronic Theses and Dissertations

Host-pathogen interactions in smut fungi are characterized by effector proteins and their role in infection. The goal of this work is to better understand these interactions by further characterizing an effector from Microbotryum lychnidis-dioicae and heterologously expressing this and other effectors in heterologous species to see if the range of these effectors can play a role in pathogenicity when expressed in distantly and closely related fungal species. These effectors have been attuned over time to their host plant but have homologous interactors in other plant species. In this study, Microbotryum lychnidis-dioicae, Sporisorium reilianum, and Ustilago maydis, were all used …

Tumor Infiltrating Lymphocytes: From Discovery To Clinical Application., Lewis Clayton Chew

Electronic Theses and Dissertations

This thesis explores the evolution of TIL therapy from its discovery to clinical application, focusing on its use in treating metastatic cancer. Adoptive cell therapy (ACT), particularly TIL therapy, has emerged as a promising alternative to traditional treatments like chemotherapy. This review article traces the historical development of TILs, highlighting key experiments and clinical trials that have shaped its understanding and application as a therapeutic strategy for patients with metastatic and difficult to treat cancers including melanoma. Importantly, this article highlights recent advancements, including the FDA approval of Lifileucel, and explores emerging strategies to enhance therapeutic efficacy of TILs through …

Evaluation Of Food-Safe Silicone Rubber Containment For Seedling Emergence, Yield, And Reusability, Claudia Garrido-Ruiz, Juan D. González-Teruel, Bruce Bugbee, Scott B. Jones

NASA

Flexible containment materials are essential for repeated planting cycles in the Utah Reusable Root Module (URRM), a zero-discharge system developed for space-based crop production. In previous trials, a vinyl top cover provided effective evaporative control and media retention but could not meet flight certification. To identify a suitable alternative, this study evaluated food-safe, flight-qualifiable silicone rubber sheets as an impermeable top cover for a wedge-based seeding system. The silicone rubber was tested at three thicknesses (1/32", 2/32", and 3/32") in combination with three linear fringe lengths (1.0", 1.5", and 2.0") and two fringe widths (0.25" and 0.5") and compared with …

Ground-Based Evaluation Of The Utah Reusable Root Module: Summary Of Three Crop Cycles With Mizuna, Tomato, And Lettuce, Claudia Garrido-Ruiz, Juan D. González-Teruel, Chihiro Dixon, Sarah Schreck, Bruce Bugbee, Scott B. Jones

NASA

The Utah Reusable Root Module (URRM) is a zero-discharge plant-growth system designed for microgravity. We evaluated its performance across four consecutive ground-based crop cycles with mizuna (Brassica rapa var. nipposinica), dwarf ‘Rejina Red’ tomato (Solanum lycopersicum), and ‘Outredgeous’ lettuce (Lactuca sativa). Five root modules (RMs) used distinct top-cover/containment designs with peatmoss medium. Growth conditions were 23–28 ^°C, 16/8 h photoperiod, PPFD ≈ 800 µmol m^⁻² s^⁻¹, RH ~ 50– 60%, and air velocity ~ 0.8 m s^⁻¹. Redundant moisture sensing (upper CS650, lower TEROS ONE) enabled accurate water-use accounting; slopes …

Abiotic Stress Impacts On Macrophomina Root Rot And Nano-Encapsulated Citrus Oils To Control Fruit And Foliar Pathogens Of Strawberry, Marina J. Gutierrez

Master's Theses

Disease remains one of the major constraints to the productivity and economic success of the California strawberry industry. The most important soilborne pathogen is Macrophomina phaseolina, causing Macrophomina root rot (MRR). The most economically significant airborne pathogens include Botrytis cinerea, causing Botrytis fruit rot (BFR), and Podosphaera aphanis, causing powdery mildew (PM).

Disease severity of MRR is highly dependent on environmental factors. Therefore, understanding which abiotic stressors contribute most to disease development is crucial for effective management. A two-year field experiment at Cal Poly, SLO (2024 and 2025) evaluated the effects of drought stress, two elevated chloride …

Synthesis Of Antibody Functionalized Polyaniline/Polystyrene/N-Gqds Composite Fibermats For Sweat Based Cortisol Biosensing Applications, Ashwin James

Honors Theses

Noninvasive, wearable biosensors capable of detecting stress biomarkers in sweat require electrodes that are flexible, conductive, insoluble in water, and highly sensitive at low concentrations of analyte. In this work, bovine serum albumin blocked and anti-cmab immobilized nitrogen doped graphene quantum dots integrated polyaniline/polystyrene composite fibermat electrodes (BSA/Anti-Cmab/N-GQDs/PANI/PS electrodes) were synthesized and evaluated as a potential platform for an electrochemical cortisol biosensor in sweat based systems. Polyaniline was utilized in order to provide electrical conductivity, while polystyrene served as a carrying polymer for mechanical support and its hydrophobicity. Composite fibermats were manufactured through Forcespinning™, followed by a secondary polyaniline graft …

Let’S Talk Mental Health: An Assessment Of Oregon Agricultural Stakeholders Discussing Mental Health, Whitney Stone, Lauren Chase, Taylor Foerster

Journal of Human Sciences and Extension

Working in agriculture presents some unique mental health stressors, including financial difficulties, climate variability, and isolation. Currently, Extension offers mental health literacy programming addressing mental health and suicide in agricultural communities; however, some of the programming is underattended, and resources are underutilized. We sought to find out how communicating about mental health resources was perceived by agricultural stakeholders who work closely with farmers and ranchers, including agribusiness employees, leaders in the community, and pre-service agriculture teachers. Through this mixed-method study using focus groups and a survey, we wanted to find out the perceptions of mental health resources to help inform …

Rural Mental Health Challenges: Addressing Community-Specific Needs Of Rural Mississippians, Mary Marshall Waller, Mary Nelson Robertson, Holli H. Seitz, Michael R. Nadorff, David R. Buys

Journal of Human Sciences and Extension

America’s mental health crisis uniquely impacts rural areas. Challenges surrounding intention and accessibility to engage in mental health-fostering behaviors exacerbate disparities in rural mental healthcare. In this study, Cooperative Extension personnel assess rural Mississippians’ intention to engage in mental health-fostering behaviors, accessibility to mental health-fostering behaviors, and helpfulness of increased access to resources for mental health challenges to determine if relationships exist between these variables. Rural Mississippi adults were recruited via an online survey panel to complete a 147-item web-based questionnaire (n = 274). Findings suggest rural adults’ intention to engage in mental health-fostering behaviors favored connecting with family and …

The Effects Of The Cooking For One Curriculum On Nutrition, Food Safety, Food Resource Management, And Physical Activity Behaviors: A Pilot Study, Tyler B. Becker Dr., Ronald L. Gibbs Jr, Becky Henne, Erika Garner

Journal of Human Sciences and Extension

The number of single adult households has increased over the previous decades, and few health education interventions address these unique living situations, which come with various economic challenges. The Cooking for One curriculum is a cooking-based intervention designed to improve nutrition, food safety, food resource management, and physical activity (PA) behaviors among single adults. This curriculum includes six lessons, each with an educational and hands-on cooking component. Participants included 1,546 adults recruited through Supplemental Nutrition Assistance Education programs in the state of Michigan. Each of the participants underwent the six-lesson Cooking for One curriculum. Prior to and following the intervention, …

Foundational Phases For Community Development: A Model For Enhancing Cooperative Extension’S Role Within The Community, George Grant, Kristina Hains

Journal of Human Sciences and Extension

The Cooperative Extension System (CES) plays a vital role in community education and development across the United States. The objective of this article is to demonstrate the historical and present-day significance of the CES as a catalyst for community development practice, while overlaying a community development model applicable to CES. A literature review is provided on the historical origins, organizational structure, and leadership responsibilities of agents and specialists relevant to community development research and practice. The researchers suggest a novel conceptual model, the Foundational Phases for Community Development, to assist agents and specialists across all areas of programming for engaging …

Bridging The Communication Gap In Natural Disaster Information: An Exploratory Study Of Extension Service Websites, Emmanuel Chisom Okolo, Rafael Landaverde, Laxmi Prasanna Kandi, Kim E. Dooley, Sarah Mccord, Kelsi Opat, Abdul Saboor Rahmany

Journal of Human Sciences and Extension

Natural disasters are expected to become more frequent and severe, increasing the need for effective disaster management. Governments are emphasizing community awareness and local self-reliance, with the Cooperative Extension Services (CES) playing a vital role in distributing science-based knowledge and supporting preparedness efforts. This qualitative content analysis (QCA), guided by Framing Theory, examined how CES disseminated weather-related disaster preparedness and response information from 2003 to 2023 on Extension Service websites. We explored: (1) the frequency of information on natural disaster preparedness and response published on Extension Service websites, (2) dissemination methods, and (3) the quality of disaster preparedness and response …

Full Issue, Volume 13, Number 3, Donna J. Peterson Dr., Scott Cummings, Elizabeth Gregory North

Journal of Human Sciences and Extension

No abstract provided.

Optimizing Endothelial Cell Specific Polymersome-Based Atheroprotective Therapies, Ally C. Brawner

All Theses

Atherosclerotic cardiovascular disease is the number one cause of death in the United States and the world. Every year, billions of dollars are spent in the US alone for direct treatments of this disease, especially on prescription medicines like statins. However, these medications have been unable to curtail global mortality rates, partially because they do not target the entirety of atherosclerotic pathophysiology. A key area of this pathophysiology is that atheroprone arteries typically include pro-inflammatory endothelial cells that exhibit decreased levels of apoAI-mediated cholesterol efflux and an increased release of adhesion molecules, such as VCAM-1. The objective of my project …

Exploring The Growth Dynamics Of Streptococcus Mutans In Diverse Environmental Conditions, Vanessa Escudero

Theses and Dissertations

Dental caries, a prevalent oral health issue, is primarily associated with the growth of Streptococcus mutans, a Gram-positive bacterium that grows under various oral environments. The primary objective of this study was to evaluate the impact of different beverages and mechanical cleaning on the growth of S. mutans, which plays a crucial role in tooth decay. Using a nano-spectrophotometer we analyzed bacterial growth by constructing growth curves under different conditions, including exposure to cranberry juice, Hawaiian juice, and mechanical brushing.

The study was designed to mimic real-world oral conditions by testing S. mutans in four distinct environments. First, we established …

Stocking Density Effects On Growth Performance Of Glass Eels (Anguilla Bicolor Pacifica) In Outdoor Tank Conditions, Frolan A. Aya

The Philippine Agricultural Scientist

Optimizing stocking density is necessary to maximize the production efficiency of farmed fish. This study examined the effect of stocking density on the growth performance of the Pacific shortfin eel Anguilla bicolor pacifica reared in outdoor tank conditions. Glass eels (0.72 ± 0.03 g mean body weight [BW]) were stocked at 50 and 100 individuals m⁻² in 6 4-m² outdoor tanks with a flow-through system set up in triplicate. Eels were individually measured for total length and BW, followed by blood sampling at the end of the 286-d trial. Glass eels stocked at a density of 50 m …

Anti-Inflammatory Effects In Apio Tuber, Jeehye Seo, Oluwatobi Amusat, Yu-Yun Wang, Annegret Jannasch, Sun-Ok Lee

Food Science Undergraduate Honors Theses

Inflammation is a crucial component of the body’s defense system but can lead to chronic diseases when uncontrolled. Diets rich in anti-inflammatory compounds are recognized as an effective strategy for mitigating chronic inflammation. This study investigated the anti-inflammatory potential of Apio (Arracacia xanthorrhiza), an underutilized Andean tuber. The goal of this study was to explore the health-promoting properties of Apio tuber, which could expand its commercial value to support local farmers and contribute to more sustainable agricultural practices.

Phenolic compounds were extracted from white (WAT) and yellow Apio tuber (YAT), and Total Phenolic Content (TPC) and antioxidant capacity …

Patch Mow Grazing: Investigating The Effects Of Mowing On Forage Nutritive Value, The Biomass–Nutritive Value Relationship, And Herbivore Selection, Trace R. Stauble

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Herbivore foraging behavior plays a critical role in shaping grassland structural heterogeneity, with selection often driven by forage biomass and nutritive value. Currently, it is thought that herbivores primarily select areas that are greater in nutritive value, and low in biomass. More specifically, disturbances like fire and grazing have been thoroughly studied showing that recently burned and grazed areas are not only higher in nutritive value but disproportionately selected by herbivores in comparison to undisturbed areas. However, most studies address only narrow ranges of defoliation, comparing intensely defoliated sites (< 10 cm) to those that are either not defoliated or have experienced a much longer time since defoliation, thereby decreasing grazing pressure as biomass increases. However, one of the most widely used methods of defoliation in grasslands, mowing, hasn’t been thoroughly studied for its effects on nutritive value, and how it impacts herbivore selection. Our study aimed to (1) evaluate how mowing treatments—i.e., short (5 cm), medium (18 cm), and tall (>30 cm, unmown)—affect nutritive value and herbivore selection, (2) assess …

Post-Fire Soil Health Assessment Of The Grasslands And Woodlands In The Semi-Arid Nebraska Sandhills, Stephanie M. Lugo

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Grasslands in the Nebraska Sandhills are shaped by natural wildfires, which help maintain the structure and function of native grasslands. In recent decades, afforestation with ponderosa pine and eastern redcedar has introduced new woodlands that altered the region’s fire dynamics and raised important questions about the effects of wildfire on different vegetation types. As wildfires become more frequent, understanding their impacts on soil health in grasslands and woodlands becomes increasingly important. This study examines the short-term effects of the 2022 Bovee Fire on physical, chemical, and biological soil properties in native grasslands and ponderosa pine and eastern redcedar afforested woodlands. …

Functional Characterization Of The Hssb1 K94q Mutation, Isaiah Singleton

All Theses

Genome stability depends on precise DNA repair pathways such as homologous recombination (HR), where human single-stranded DNA-binding protein 1 (hSSB1) stabilizes single-stranded DNA (ssDNA) intermediates and recruits repair complexes. Post-translational modifications, including acetylation, are known to regulate hSSB1 activity, but their molecular effects remain poorly defined. This study examined the role of lysine 94 (K94) acetylation using a glutamine substitution mutant (K94Q) that mimics the acetylated state. Recombinant hSSB1 wild type (WT) and hSSB1_K94Q were expressed in Escherichia coli and purified using Ni–NTA affinity chromatography. Biochemical analyses, including electrophoretic mobility shift assays (EMSA), single-strand annealing assays, and displacement loop …

Establishment Of Native Warm-Season Forages Using Organic Practices, Kolten Ray Wright

Graduate Theses and Dissertations

Abstract Grassland agriculture is seen as a particularly sustainable form of agriculture. Many opportunities are known to use organic production methods or incorporate native warm-season grasses (NWSG) to accomplish both production goals and environmental or social outcomes. Environmental concerns have led some practitioners to question the use of organic methods to establish NWSG. As limited work has been accomplished in the northern bermudagrass belt, this project sought to compare organic establishment strategies for common NWSG species. A field trial conducted at the USDA ARS Dale Bumpers Small Farms Research Center in Booneville, AR found that NWSG establishment success in the …

Using Color And Fluorescent Spectrometry As A New Rapid Cleaning Verification Method In Poultry Processing Plants, Clark Griscom

Graduate Theses and Dissertations

The poultry industry is expected to be growing at 3% in the total global market with the U.S. being the largest producer of poultry meat. Poultry processing plants are expected to produce 140 birds per minute with the potential of up to 175 birds per minute to meet the market demand. With the growing market and need for faster production, maintaining food safety needs to be a priority to ensure the safety of consumers. Over 9.9 million foodborne illness cases are reported each year in the United States. A change in food safety is needed, and one area lacking is …

Micro-Greens, Not Micro-Yellows: Evaluating Dehydration-Based Preservation Techniques For Reducing Foodborne Pathogens And Quality Preservation On Microgreens, Travis S. Sananikone

Graduate Theses and Dissertations

Microgreens are increasingly popular among consumers due to their high nutritional value. However, their limited shelf-life remains a significant challenge. To extend their usability, small-scale growers increasingly apply home preservation methods like warm air drying or freeze-drying. However, the effects of these methods on common foodborne pathogens are not well understood. Radish and alfalfa microgreens were inoculated with cocktails of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes 4b (ca. 8.0 log CFU/g). Treatments included dehydration in a commercial dehydrator, freeze-drying (-20°C, 24 h), and convective drying in an oven (60°C for 4 h), and convective drying in an oven …

Expression, Purification And Characterization Of Full Length Tdp-43, Danya Abouzaid

Honors Theses

TAR DNA-binding protein 43 (TDP-43) is a 43 kDa RNA-binding protein linked to several neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and Alzheimer’s disease. 5-7,28,30,33 Studying full-length TDP-43 has been difficult because of its intrinsic instability and tendency to aggregate.19,25,28,33,36 In this study, we focused on overcoming the difficulties to express full-length TDP-43 in E. coli recombinantly. We focused on expressing full-length TDP-43 as a fusion construct with a maltose-binding protein (MBP) to improve solubility and stability. Our strategy was to purify TDP-43-MBP fusion and make biomolecular condensates with RNA before cleaving the tag with thrombin protease. …

Effectiveness Of Education On Increasing Training Likelihood: An Analysis In Relation To Physique Inflation, Reid J. Venable

Honors College Theses

Resistance training is a widely recommended form of exercise for enhancing muscular strength, improving metabolic health, and promoting overall quality of life. In addition to these benefits, perhaps the most popular reason for participating in resistance training is to achieve muscle hypertrophy and attain a desired or aesthetic physique. However, various factors such as the use of performance-enhancing drugs and social media have led to “physique inflation,” a cultural phenomenon in which exposure to highly enhanced or unrealistic body types shifts perceptions of what is considered a “normal” or attainable physique. As a result, many individuals avoid or delay resistance …

Fusarium Equiseti Pathogen Of Potato In Kenya And Its Potential In Vitro Biocontrol Using Fungal Endophytes, Marie Cecile Muhorakeye, Fathiya M. Khamis, Everlyne Samita Namikoye, Komivi S. Akutse

All Peer-Reviewed Publications

Accurate pathogen diagnosis is fundamental for effective crop disease management. In Kenya, a pathogen causing significant damage in potato farms was initially misidentified as Phytophthora infestans. This study aimed to correctly identify this pathogen and explore initial control measures using a polyphasic approach. The methodology integrated morphological observation, pathogenicity testing, and molecular analysis using ITS and EF1-α gene sequencing. The results confirmed the pathogen’s identity as Fusarium equiseti, with morphological features consistent with this species and molecular sequencing showing 99.6% identity to reference strains. This is the first official report of Fusarium equiseti as a potato pathogen in Kenya. Furthermore, …

Impact Of Thermodynamic Factors And Intrinsic Properties On Crystallization Behavior And Physical Properties Of Edible Fats, Nabila Anjum

All Graduate Theses and Dissertations, Fall 2023 to Present

Crystallization behavior of three edible fats: interesterified soybean oil (IESBO), interesterified palm olein (IEPO), and fully hydrogenated palm kernel oil (FHPKO) were explored to understand how this process is affected by different factors. The study focused on how the chemical composition of these fats affects their behavior like how much time they require to be crystallized at a certain temperature. By using different scientific methods, how quickly each fat began to crystallize and how much energy it took to start the process was measured. Among the methods used, Gompertz equation, a mathematical model, gave the most consistent and reliable results …

Development Of Novel Small Molecule Therapeutics For Myotonic Dystrophy, Xhesika Azis

Biological Sciences

Myotonic Dystrophy type 1 (DM1) results from expanded CUG repeat RNA that sequesters MBNL proteins and disrupts alternative splicing. Small molecules that bind toxic repeat RNA and release MBNL proteins represent a promising therapeutic strategy. This thesis examines newly synthesized modified polycyclic compounds (MPCs) engineered to target CUG RNA with enhanced specificity and efficacy. Through Suzuki–Miyaura coupling and benzoquinone-mediated cyclization, MPC03 was synthesized and characterized by NMR and LC–MS.

The biological activity of these compounds was assessed in DM1 patient-derived myotubes and fibroblasts. MPC03 consistently produced dose-dependent rescue of multiple MBNL-regulated exons, restoring splicing patterns toward those observed in healthy …

The Genetic And Environmental Determinants Of Stress Resilience Across Model Systems, Carson Len Stacy

Graduate Theses and Dissertations

Survival in fluctuating environments demands that organisms effectively anticipate and respond to future challenges. This capacity is shaped by both genetic background and environmental context. In this dissertation, I apply a population aware lens to explore how genetic diversity and environmental conditions intersect to shape stress survival. Integrating functional genomics and transcriptomics in Saccharomyces cerevisiae (brewer’s yeast) and a vertebrate (common canary) system, I dissect gene-by-environment (GE) interactions that underpin resilience. First, I move beyond a single reference genome by using a pan-genomic CRISPR knockout screen to assess osmotic stress survival across diverse S. cerevisiae isolates. This analysis reveals a …

An Update On Novel Taxa And Revised Taxonomic Status Of Bacteria Isolated From Human Clinical Specimens Described In 2024, Arianna Carella, Karen C. Carroll, Erik Munson

Clinical Lab Sciences Faculty Research and Publications

Knowledge of novel and revised bacterial taxonomy facilitates routine operations in the medical microbiology laboratory and communication with important stakeholders. Notable new gram-positive taxa accepted in 2024 include the clinically significant Staphylococcus brunensis sp. nov., as well as Streptococcus suis subsp. hashimotonensis subsp. nov., which can be delineated from other Streptococcus suis subsp. by possession of the Lancefield group A antigen. Four novel Enterobacterales species are discussed, plus a novel eighth family Gallaecimonadaceae. One of these, Providencia huashanensis sp. nov. is clinically significant and harbors multiple genetic determinants that encode antimicrobial resistance mechanisms. Stenotrophomonas forensis sp. nov., derived from …