Digital Commons Network^™

Spatial Ecology Of Translocated Raccoons, Jacob E. Hill, James L. Helton, Richard B. Chipman, Amy Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes

United States Department of Agriculture Wildlife Services: Staff Publications

Raccoons (Procyon lotor) are routinely translocated both legally and illegally to mitigate conflicts with humans, which has contributed to the spread of rabies virus across eastern North America. The movement behavior of translocated raccoons has important ramifications for disease transmission yet remains understudied and poorly quantified. To examine the spatial ecology of raccoons following experimental translocation, we performed reciprocal 16 km-distance translocations of 30 raccoons between habitats of high and low raccoon density (bottomland hardwood and upland pine, respectively) across the Savannah River Site (SRS) in Aiken, South Carolina, USA (2018–2019). Translocation influenced patterns of raccoon space use, …

Evaluation Of Oral Baits And Distribution Methods For Tasmanian Devils (Sarcophilus Harrisii), Sean Dempsey, Ruth J. Pyer, Amy Gilbert, Nicholas M. Fountain-Jones, Jennifer M. Moffat, Sarah Benson-Amram, Timothy J. Smyser, Andrew S. Flies

United States Department of Agriculture Wildlife Services: Staff Publications

Context

Diseases are increasingly contributing to wildlife population declines. Tasmanian devil (Sarcophilus harrisii) populations have locally declined by 82%, largely owing to the morbidity and mortality associated with two independent transmissible devil facial tumours (DFT1 and DFT2). Toxic baits are often used as a management tool for controlling vertebrate pest populations in Australia, but in other areas of the world, oral baits are also used to deliver vaccines or pharmaceuticals to wildlife.

Aim

Our goal was to evaluate the potential use of edible baits as vehicles for vaccine delivery to Tasmanian devils.

Method

We first tested bait palatability with …

Comparison Of Daily Activity Patterns Across Seasons Using Gps Telemetry And Camera Trap Data For A Widespread Mammal, David W. Wolfson, Peter E. Schlichting, Raoul K. Boughton, Ryan S. Miller, Kurt C. Vercauteren, Jesse S. Lewis

United States Department of Agriculture Wildlife Services: Staff Publications

Daily activity patterns of animals can be dynamic across seasons in response to changing environmental conditions. Daily activity, though, has rarely been evaluated in relation to multiple factors (for example, seasons, demographics, and data collection methods), which could be important for understanding what drives activity patterns. Here, we evaluated the daily activity patterns of a widespread invasive species, wild pigs (Sus scrofa), across two ecologically different study areas at Buck Island Ranch, Florida, and Tejon Ranch, California (United States), from 2015 to 2018. Using GPS telemetry data (62 individuals in Florida, 21 individuals in California) and detections from …

Are Rabid Raccoons (Procyon Lotor) Ready For The Rapture? Determining The Geographic Origin Of Rabies Virus-Infected Raccoons Using Radcapture And Microhaplotypes, Matthew W. Hopken, Antoinette J. Piaggio, Zaid Abdo, Richard B. Chipman, Clara P. Mankowski, Kathleen M. Nelson, Mikaela Samsel Hilton, Christine Thurber, Mirian T. N. Tsuchiya, Jesús E. Maldonado, Amy T. Gilbert

United States Department of Agriculture Wildlife Services: Staff Publications

North America is recognized for the exceptional richness of rabies virus (RV) wildlife reservoir species. Management of RV is accomplished through vaccination targeting mesocarnivore reservoir populations, such as the raccoon (Procyon lotor) in eastern North America. Raccoons are a common generalist species, and populations may reach high densities in developed areas, which can result in contact with humans and pets with potential exposures to the raccoon variant of RV throughout the eastern United States. Understanding the spatial movement of RV by raccoon populations is important for monitoring and refining strategies supporting the landscape-level control and local elimination of …

Promiscuous Feeding Across Multiple Honey Bee Hosts Amplifies The Vectorial Capacity Of Varroa Destructor, Zachary S. Lamas, Serhat Solmaz, Eugene V. Ryabov, Joseph Mowery, Matthew Heermann, Daniel Sonenshine, Jay D. Evans, David J. Hawthorne

Biological Sciences Faculty Publications

Varroa destructor is a cosmopolitan pest and leading cause of colony loss of the European honey bee. Historically described as a competent vector of honey bee viruses, this arthropod vector is the cause of a global pandemic of Deformed wing virus, now endemic in honeybee populations in all Varroa-infested regions. Our work shows that viral spread is driven by Varroa actively switching from one adult bee to another as they feed. Assays using fluorescent microspheres were used to indicate the movement of fluids in both directions between host and vector when Varroa feed. Therefore, Varroa could be in either …

Frankenbacteriosis Targeting Interactions Between Pathogen And Symbiont To Control Infection In The Tick Vector, Lorena Mazuecos, Pilar Alberdi, Angélica Hernández-Jarguín, Marinela Contreras, Margarita Villar, Alejandro Cabezas-Cruz, Ladislav Simo, Almudena González-García, Sandra Díaz-Sánchez, Girish Neelakanta, Sarah I. Bonnet, Erol Fikrig, José De La Fuente

Biological Sciences Faculty Publications

(Summary) Tick microbiota can be targeted for the control of tick-borne diseases such as human granulocytic anaplasmosis (HGA) caused by model pathogen, Anaplasma phagocytophilum. Frankenbacteriosis is inspired by Frankenstein and defined here as paratransgenesis of tick symbiotic/commensal bacteria to mimic and compete with tick-borne pathogens. Interactions between A. phagocytophilum and symbiotic Sphingomonas identified by metaproteomics analysis in Ixodes scapularis midgut showed competition between both bacteria. Consequently, Sphingomonas was selected for frankenbacteriosis for the control of A. phagocytophilum infection and transmission. The results showed that Franken Sphingomonas producing A. phagocytophilum major surface protein 4 (MSP4) mimic pathogen and reduce infection …

Dna Methylation And The Response To Infection In Introduced House Sparrows, Melanie Gibson

College of Graduate Studies: Theses & Dissertations

Epigenetics is the study of molecular modification of a genome without changing its base pairs. The most studied type of epigenetic mechanism is DNA methylation, which is capable of turning a gene “on” or “off.” Epigenetic potential is the capacity to which an individual can have methylation on its genome. The more CpGs available, the greater the epigenetic potential. In invasive species, genetic variation has been observed to be paradoxical: not much of it exists on a genomic level, but epigenetically, phenotypic variation can occur. The focus on shift in gene expression in this study is on Toll-Like Receptor 4 …

Effects Of Occupational Tasks And Personal Protective Equipment On Static And Functional Balance Among Career Firefighters, Petra Kis

College of Graduate Studies: Theses & Dissertations

BACKGROUND: Musculoskeletal injuries are commonly incurred on the fireground among firefighters. Most injuries are attributable to overexertion, fall, jump, slip, and trip mechanisms. These injuries may be caused by many factors, including the independent and collective effects of wearing personal protective equipment (PPE) and the fatigue induced by performing occupational tasks. Therefore, the purpose of this study was to investigate the effect of PPE and fire suppression tasks on static and functional balance among firefighters. METHODS: 32 healthy male career structural firefighters (Age: 31.8±9.5 yr) volunteered for this study. Static and Functional Balance Tests (FBT) were performed in …

The Hepatic Immunological Pattern Shaped By Dominant-Subdominant Cellular Interactions Creates A Collective Function Beyond The Function Of Each Cellular Constituent To Orchestrate Progression Of Hepatocellular Carcinoma, Nicholas J. Koelsch

Theses and Dissertations

Abundance of data on the role of inflammatory immune responses in the progression or inhibition of hepatocellular carcinoma (HCC) has failed to offer a curative immunotherapy for HCC. This is largely because of taking reductionist approaches and missing the collective function of the hepatic immune system by focusing on specific immune cell types. To this end, we propose that focusing on the dominant-subdominant patterns of the immune cells would allow understanding of the mechanism by which a collective immune function emerges. To identify the collective immune function through a systems immunology perspective, we performed high-throughput analysis of snRNAseq data collected …

Studying Gene Function In Streptococcus Sanguinis Using Rna-Sequencing Analysis, Ahmed Ismail

Theses and Dissertations

The oral cavity hosts hundreds of bacterial species, amongst them are the Streptococci family. More specifically, S. sanguinis is a benign bacteria in the oral cavity to protect the tooth surface from S. mutans. which is a pathogen causing dental caries. While S. sanguinis is harmless in the oral cavity, it becomes pathogenic once entering the bloodstream and causing Infective Endocarditis (IE). To further study S. sanguinis and eventually develop an effective drug to treat IE caused by S. sanguinis, a gene expression study was conducted. In order to study gene expression, RNA-sequencing was conducted, and appropriate methods to remove …

Cryogenic X-Ray Crystallographic Studies Of Biomacromolecules At Turkish Light Source "Turkish Delight", Necati̇ Atalay, Enver Kami̇l Akcan, Mehmet Gül, Esra Ayan, Ebru Destan, Fatma Betül Ertem Kuzucu, Nuretti̇n Tokay, Bariş Çakilkaya, Zeli̇ş Nergi̇z, Gözde Karakadioğlu Usta, Abdullah Kepceoğlu, İlki̇n Yapici, Bi̇lge Tosun, Ni̇lüfer Baldir, Günseli̇ Yildirim, J Austin Johnson, Ömür Güven, Alaleh Shafiei, Nazli Eylül Arslan, Merve Yilmaz, Cahi̇ne Kulakman, Seyi̇de Seda Paydos, Seynep Sena Çi̇nal, Kardelen Şabanoğlu, Ayşegül Pazarçevi̇ren, Ayşenur Yilmaz, Başak Canbay, Bengi̇su Aşçi, Esra Kartal, Serra Tavli, Mehmet Çaliseki̇, Günce Göç, Ari̇f Mermer, Gamze Yeşi̇lay, Sevde Altuntaş, Hiroshi Tateishi, Masami Otsuka, Mikako Fujita, Şaban Teki̇n, Hali̇li̇brahi̇m Çi̇ftçi̇, Serdar Durdaği, Gi̇zem Di̇nler Doğanay, Ezgi̇ Karaca, Burcu Kaplan Türköz, Burak Veli̇ Kabasakal, Ahmet Kati, Hasan Demi̇rci̇

Turkish Journal of Biology

X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g., proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryocrystallography has never been performed in Türkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, İstanbul, Türkiye will enable Turkish and international researchers to easily perform high-resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and …

Interactions Between Hiv And Opioids On Antiretroviral Accumulation, The Blood Brain Barrier, And The Inflammatory Response In The Brain., Kara Rademeyer

Theses and Dissertations

The complex mechanisms related to HIV infection, neurodegeneration, and chronic neuroinflammation collectively describe neuroHIV (Hauser et al. 2007; Chang et al. 2014; Smith et al. 2014). Specifically, opioid abuse, poor penetration of antiretroviral (ARV) drugs, chronic inflammation and neuronal injury/degeneration are all implicated in neuroHIV (Fantuzzi et al. 2003; Letendre et al. 2004; Verani et al. 2005; Duncan and Sattentau 2011; Hong and Banks 2015; Simoes and Justino 2015; Olivier et al. 2018; Murphy et al. 2019; Osborne et al. 2020). For the first time, we demonstrate that morphine, fentanyl, and methadone in vivo alter the brain accumulation of ARVs, …

The Impact Of Hiv-1 Tat And Morphine On Spatial Distribution Of Drugs In The Brain., Austin M. Jones

Theses and Dissertations

Despite combination antiretroviral therapy effectively suppressing HIV within the periphery, the central nervous system (CNS) remains affected by the virus. Approximately half of people living with HIV will experience HIV-associated neurocognitive disorders (HAND) or neuroHIV. Concurrent opioid use exacerbates neuroHIV by promoting neuroinflammation, viral replication, and potentially altering the antiretroviral concentrations within the brain.

Using a transgenic mouse that expresses the HIV-1 Tat protein, we examined the effects of Tat and morphine on antiretroviral accumulation and distribution and the effects of Tat on morphine accumulation within the brain using infrared matrix-assisted laser desorption electrospray ionization with mass spectrometry imaging (IR-MALDESI-MSI). …

Molecular Phylogenetics Of Aedes Aegypti (L., 1762) (Diptera: Culicidae) In Eastern Black Sea Area Of Turkey And Possible Relations With The Caucasian Invasion, Murat Öztürk, Muhammet Mustafa Akiner

Turkish Journal of Zoology

Aedes aegypti (L., 1762) (Diptera: Culicidae) is an important pest for human health. It vectors many diseases, including yellow fever (YF), dengue (DENV), and chikungunya (CHIKV). This species has invaded numerous countries including Balkan and Caucasian countries few past decades. The established populations of species were found in Turkey in 2015. We investigated the genetic variation, molecular phylogenetics, and differences between samples from Turkey and Georgia using four mitochondrial and one genomic DNA markers. The possible origin of the species was determined separately for each gene region using maximum likelihood trees. The ML analysis showed a close relation with the …

Evaluating The Contribution Of Adjacent Charges On The Observed Ph-Dependent Binding Profile For A Vhh/Antigen Interaction With A Linked Histidine Protonation Equilibria, Kiruthika Arulmoly

Graduate Research Theses & Dissertations

pH dependent protein-protein interactions are of great importance for various biological processes. In addition, the generation of engineered pH-dependent therapeutic antibodies opens opportunities for the extension of therapeutic half-life, thereby reducing drug dosage or frequency of administration, as well as providing pH-dependent targeting, such as the tumor microenvironment. Engineering pH-dependent antibody-antigen interaction is mostly achieved through the incorporation of ionizable residues within the antigen binding interface. These ionizable residues must undergo a change in environment upon forming a protein-protein complex, which causes a change in the ionizable group’s pKa value. This coupled protonation equilibria is responsible for the observed pH-dependent …

Lactobacillus Metabolite Nc As An Inflammation Inhibitor, Drew Hahnnah Landrowski

Master's Theses

The human body contains a diverse ecosystem of microbes. Commensal bacteria have numerous mutually-beneficial relationships with their host. Not only do they assist in digestion and gut homeostasis, they are critical for development and function of the immune system. As such, hosts and microbes alike have evolved mechanisms to foster symbiosis. Gut barrier function, toll-like receptor signaling, and metabolites are all examples of how hosts interact with microbes to promote this mutually-beneficial relationship. Metabolites from commensal bacteria promote the generation of regulatory T-cells, capable of ameliorating inflammation. A number of inflammatory, allergic, and autoimmune conditions are associated with dysbiosis. Therefore, …

Identification And Creation Of Novel Bacteriocins With Potential Food And Clinical Applications, David Lynch

Theses

Bacteriocins represent a heterogeneous group of small, ribosomal-synthesized, potent antibacterial peptides produced by bacteria, capable of inhibiting bacteria both closely related or indeed those from other genera than the producer. In addition to their role as food preservatives, bacteriocins have potent activity against medically significant pathogens and are considered attractive alternatives to antibiotics, due to their inherent heat stability, potency at nanomolar scale, resistance to proteases and low levels of acquired resistance in commercial applications. The aim of this thesis was to identify, purify and bioengineer novel bacteriocins from Staphylococcus and Lactic Acid Bacteria (LAB), with a view to expanding …

A Year At The Forefront Of Bacterial Defense Systems Against Neutrophilic Oxidants, Sadia Sultana, Jan-Ulrik Dahl

Faculty Publications – Biological Sciences

One challenge for invading pathogens represents the exposure to highly microbicidal hypohalous acids (HOX), such as hypochlorous acid (HOCl) and hypothiocyanous acid (HOSCN). Generated at high concentrations by innate immune cells during phagocytosis, HOX kills the engulfed microbes through extensive macromolecular damage. However, microorganisms have evolved strategies to detoxify the oxidants and/or alleviate HOX-mediated damage, which improves their survival during HOX exposure. Many of these defense systems are bacteria-specific and therefore considered potential drug targets. Our minireview highlights recent (July 2021 to November 2022) advances in the field of microbial HOX defense systems and how these systems are regulated. We …

Documenting Range Expansion Of Two Invasive Tick (Acari:Ixodidae) Species In Eastern South Dakota, Braden Wojahn

Dissertations and Theses

This project documents the expanded zoogeographic ranges of the blacklegged tick (Ixodes scapularis) and lone star tick (Amblyomma americanum) within eastern South Dakota. Ticks are important when considering a “One Health” approach, as many species are competent vectors of zoonotic pathogens causing human diseases and conditions such as Lyme disease, tularemia, rickettsia, and alpha gal syndrome. The research involved constitutes two steps. First, tick surveillance was conducted in eastern counties of South Dakota, spanning March 2021 through August 2022. Surveillance took place along edge habitat at recreation areas, state and public parks, wildlife management areas and refuges, and other natural …

Prevalence Of Batrachochytrium And Ranavirus In Southern Torrent Salamanders (Rhyacotriton Variegatus) In Northern California, Marilyn Violeta Sandoval

Cal Poly Humboldt theses and projects

Amphibian population declines around the world have been attributed in part to the emergence of deadly pathogens. However, the severity of the effects of pathogens on amphibian communities can vary by species and location. This study focuses on assessing the prevalence of Batrachochytrium dendrobatidis (Bd), Batrachochytrium salamandrivorans (Bsal), and ranaviruses in populations of southern torrent salamanders (Rhyacotriton variegatus). I also tested for association between environmental factors and the prevalence and viral load of the three pathogens in Northern California. A total of 309 skin swab samples were collected from salamanders in nine sites in …

Masks, Misinformation, And Making Do: Appalachian Health-Care Workers And The Covid-19 Pandemic, Wendy Welch

Ohio University Press Open Access Books

The firsthand pandemic experiences of rural health-care providers—who were already burdened when COVID-19 hit—raise questions about the future of public health and health-care delivery.

This volume comprises the COVID-19 pandemic experiences of Appalachian health-care workers, including frontline providers, administrators, and educators. The combined narrative reveals how governmental and corporate policies exacerbated the region’s injustices, stymied response efforts, and increased the death toll.

Beginning with an overview of the SARS-CoV-2 virus and its impact on the body, the essays in the book’s first section provide background material and contextualize the subsequent explosion of telemedicine, the pandemic’s impact on medical education, and …

Swine Research Industry, Alyssa Abendroth '24

Distinguished Student Work

My business research revolved around biosecurity and disease. My inquiry was led by the question How common are diseases in commercial swine units and how can they be prevented from a staff personnel standpoint?. I completed my research by gaining knowledge through experience in my internship, as well as a literature review. I could not find an explicit response to how common diseases are, but I did learn about biosecurity and disease prevention.

The Use Of A Habitat Quality Stress Index To Evaluate Stress As An Analog For Proximate Fitness In The American Crow Within A Matrix Of Landcover Characteristics To Assess Its Potential Contribution To Disease Etiologies, Theodore Lee Grabarz

Antioch University Dissertations & Theses

All organisms occur within spatial and temporal environments to maximize proximate fitness (health) and thus life history outcomes. Previous work has examined the temporal and behavioral aspects of proximate fitness on life history outcomes particularly regarding highly perturbed environments (i.e., climate and land use change, resource extraction, agricultural erosion, etc.). My work focuses on the less examined spatial aspect of these perturbed environments. More specifically, this dissertation examines habitat selection and quality as the basis for understanding stress response (negative and positive feedback mechanisms) to environmental stressors within the larger context of regional or gamma (ɣ) biodiversity. Through the lens …

Connecting Antibiotic Resistance To The Environment (Care): Introducing A Novel Framework Integrating Chemical Cross-Resistance And Place-Based Engagement To The Blue Marsh Watershed In Reading, Pennsylvania, Jill Felker

Antioch University Dissertations & Theses

Antibiotic resistance is a serious health threat around the world. Millions of individuals are infected with antibiotic-resistant bacteria yearly, and thousands die from previously curable illnesses. Although antibiotic resistance occurs naturally, misuse of antibiotics accelerates the loss of their effectiveness. Public health campaigns focusing on antibiotic awareness have not effectively communicated and educated the public on this health crisis. New efforts to combat antibiotic resistance are urgently needed. This dissertation focuses on the ecological and public health components of antibiotic resistance research that must be addressed to decelerate antibiotic resistance. A new interdisciplinary theoretical framework was developed to Connect Antibiotic …

In-Vivo Assessment Of A Direct-Fed Microbial On Lactation Performances, Blood Biomarkers, Ruminal Fermentation And Microbial Abundance In Transition To Mid-Lactation Holstein Cows, Marcela Bulnes Lopez

Electronic Theses and Dissertations

The transition period is a crucial stage in the lactation cycle and can lead to significant metabolic changes in cows. The use of nutritional interventions, such as direct-fed microbials, may assist cows during the transition from pregnancy to early lactation, reducing the occurrence of metabolic disorders and improving overall health. The primary goal of this study was to assess the effects of a commercial rumen-derived directfed microbial (DFM) product (Galaxis™ Frontier (GF) Native Microbials Inc., CA, USA) on various factors such as performance, blood biomarkers, rumen fermentation, and bacterial population in dairy cows during the transition period up to 100 …

Age-Induced Changes In Anti-Tumor Immunity Alter The Tumor Immune Infiltrate And Impact Response To Immuno-Oncology Treatments, Viia E Valge-Archer, Simon J Dovedi

Faculty, Staff and Student Publications

INTRODUCTION: Immuno-oncology (IO) research relies heavily on murine syngeneic tumor models. However, whilst the average age for a cancer diagnosis is 60 years or older, for practical purposes the majority of preclinical studies are conducted in young mice, despite the fact that ageing has been shown to have a significant impact on the immune response.

METHODS: Using aged (60-72 weeks old) mice bearing CT26 tumors, we investigated the impact of ageing on tumor growth as well as the immune composition of the tumor and peripheral lymphoid organs.

RESULTS: We found many differences in the immune cell composition of both the …

Wastewater Pandemic Preparedness: Toward An End-To-End Pathogen Monitoring Program, Juwan Cormier, Kristi Louise Hoffman, Catherine Troisi, Blake Hanson, Joseph Petrosino, John Balliew, Pedro A Piedra, Janelle Rios, Jennifer Deegan, Cici Bauer, Fuqing Wu, Kristina D Mena, Eric Boerwinkle, Anthony W Maresso

Faculty, Staff and Student Publications

Molecular analysis of public wastewater has great potential as a harbinger for community health and health threats. Long-used to monitor the presence of enteric viruses, in particular polio, recent successes of wastewater as a reliable lead indicator for trends in SARS-CoV-2 levels and hospital admissions has generated optimism and emerging evidence that similar science can be applied to other pathogens of pandemic potential (PPPs), especially respiratory viruses and their variants of concern (VOC). However, there are substantial challenges associated with implementation of this ideal, namely that multiple and distinct fields of inquiry must be bridged and coordinated. These include engineering, …

Survey Of West Nile And Banzi Viruses In Mosquitoes, South Africa, 2011-2018, Leo Braack, Maarten Schrama, Erin Gorsich, Antonio Paulo Gouveia Almeida, Marietjie Venter

Faculty, Staff and Student Publications

We collected >40,000 mosquitoes from 5 provinces in South Africa during 2011-2018 and screened for zoonotic flaviviruses. We detected West Nile virus in mosquitoes from conservation and periurban sites and potential new mosquito vectors; Banzi virus was rare. Our results suggest flavivirus transmission risks are increasing in South Africa.

Odd Bedfellows & Marriage Of Necessity: Public Health And Politics In American Federalism, Gabriella Victoria Chianese

Honors Theses and Capstones

In the wake of the coronavirus disease 2019 (COVID-19) pandemic, while the public disagrees over mask mandates, required vaccinations, and social distancing, it seems like one common sentiment exists – a distaste for the odd bedfellows of politics and public health. There are those who cry for the compartmentalization of the science of public health and the art of politics to rectify this situation. In the wake of so much confusion and chaos, it is not unjustified to demand the depoliticization of public health; however, this response is unrealistic given the modern political climate, demonstrates a narrow understanding of the …

Investigating Optimal Lipid:Protein Profiles For Infant Milk Formula, Valentina Ivanova

Theses

From the early twentieth century onward, much research has focused on improving infant milk formula (IMF). During the development of IMF, breast milk is commonly considered as the reference and IMF manufacturers are thriving to mimic the composition and/or to match the functionality of breast milk as closely as possible. A variety of bioactive ingredients, found in human breast milk, have been isolated and investigated for their potential functionality and utilised in the production of IMF. This study evaluated the current knowledge on compositional differences between human breast milk and IMF, and recent advances and developments in IMF production. Following …