Digital Commons Network^™

Dairy And Food Science Student Newsletter, August 8, 2025, Department Of Dairy And Food Science

Dairy and Food Science Student Newsletter

No abstract provided.

Northern Arizona Ecological Conservation: Assessing Quaking Aspen Health In Northern Arizona Using Earth Observations, Ikram Morso, Luke Collins, Margaret Cox, Melissa Schwan

Aspen Bibliography

Quaking aspen (Populus tremuloides) forests in Northern Arizona provide critical habitat for endangered bird species and support regional tourism and recreation. However, aspen populations are declining due to a combination of abiotic and biotic stressors. In response, land managers implemented strategies, such as ungulate exclusion fencing, prescribed burns, and stand thinning, but efforts are constrained by limited data on where and when to intervene. This project worked in collaboration with the U.S. Forest Service, the National Park Service, the Arizona Department of Forestry and Fire Management, and Northern Arizona University School of Forestry. The team utilized Earth observations …

Thionine Modulates Tau Phosphorylation In An Alzheimer’S Disease Cell Culture Model, Seda Önder, Kevser Bi̇beroğlu, Özden Tacal

Turkish Journal of Biology

Background/aim: Tau protein, which is crucial for sustaining the cytoskeletal network by assisting microtubule construction, contributes significantly to the pathophysiology of Alzheimer’s disease (AD). The hyperphosphorylation of tau causes it to detach from microtubules (MTs), leading to the formation of neurofibrillary tangles (NFTs) in neurons, which ultimately results in cell death. Thionine (TH), a cationic phenothiazine-structured compound, has been the topic of extensive research due to its interesting physicochemical properties. It is a common biological dye, especially useful in histology due to its strong affinity for biological membranes. Furthermore, TH serves as a photosensitizer in phototherapy. It has a phenothiazine …

Chelidonium Majus L.-Containing Gel May Improve Diabetic Wound Healing By Modulating Mmp-2, Mmp-9, And Collagen Levels, Kaan Kaltalioğlu, Eli̇f Naz Gürsoy, Barbaros Balabanli, Zeki̇ Aytaç, Şule Coşkun Cevher

Turkish Journal of Biology

Background/aim: Delayed wound healing in diabetic patients is a significant complication that reduces quality of life, prompting the continuous investigation of new therapeutic agents. This study designed to explore the dose-dependent effects of different parts of Chelidonium majus L. (CM), a medicinal plant traditionally used for skin disorders, on diabetic skin wounds.

Materials and methods: In diabetic rats, full-thickness excisional wounds were formed. CM-containing gels (aerial parts at 3%, 6%, 9% and root at 0.5%, 1%, 1.5%) were developed and applied to the wounds. After the treatment period, the rats were sacrificed, and wound healing activity was assessed macroscopically, histopathologically, …

Investigating Food Web Dynamics Of Great Lakes Coastal Wetlands Using A Novel Stable Isotope Approach, Alyssa M. Smith

Masters Theses

Coastal wetlands of the Great Lakes support abundant populations of fish, invertebrates, and vegetation, though the trophic linkages connecting primary production and lower consumers is not well understood in these systems. We implemented a multiple-tracer approach, pairing traditional food web isotope tracers like carbon (δ13C) and nitrogen (δ15N) with total mercury concentrations (THg). We predicted that filamentous algae would be the dominant energy resource in the diet of lower trophic-level invertebrates in the Grand River Estuary, a network of riverine coastal wetlands adjacent to lake Michigan. In addition, we predicted that adding THg as a tracer would improve the resolution …

Kabasura Kudineer Choornam, A Medicinal Polyherbal Formulation, Modulates Human Macrophage Polarization And Phagocytic Function, Asli Korkmaz, Duygu Ünüvar, Si̇nem Günalp, Derya Göksu Helvaci, Duygu Sağ

Turkish Journal of Biology

Background/aim: Kabasura kudineer choornam (KKC) is a polyherbal formulation of 15 ingredients. It has antiinflammatory and antimicrobial properties and are effective in managing the symptoms of H1N1 swine flu and COVID-19. However, its mechanism of action is not fully understood. In this study, we examined the effect of KKC on the polarization and function of primary human macrophages.

Materials and methods: Human monocyte-derived macrophages (M0 macrophages) pretreated with KKC extract were polarized into M1, M2a, or M2c subtypes. The expression of the M1/M2 polarization markers was analyzed using qPCR, flow cytometry, and ELISA, and the phagocytosis capacity of …

Different Hypoxic Response Of Human Cavii And Caenorhabditis Elegans Homologous Genes Cah-3, Cah-4, Cah-5, Sümeyye Aydoğan Türkoğlu, Aysu Bozkurt, Fatma Poyrazli, Derya Okuyan

Turkish Journal of Biology

Background/aim: A number of carbonic anhydrase (CA) family proteins have been implicated in cancer. They contribute to the hypoxic microenvironment. CAVII is often downregulated in colorectal carcinoma and it has been associated with increased tumor size, node metastasis, and adverse clinical outcomes. In this study, we aimed to investigate the effect of hypoxia on CAVII protein in human colon cancer and prostate cancer cells. In addition, the regulation of CAH genes in Caenorhabditis elegans was examined. These are homologous to CAVII in humans.

Materials and methods: CAVII expression was analyzed in different cell lines such as human colon cancer (SW480 …

Lactic Acid Bacteria Alleviate Neuronal Excitotoxicity And Extend Caenorhabditis Elegans Lifespan, Bao Le, Thi Nhat Hong Nguyen, Seung-Hwan Yang

Turkish Journal of Biology

Aim: Neuronal cell death plays a critical role in the development of neurological disorders associated with aging. This study aimed to evaluate the beneficial effects of heat-killed lactic acid bacteria (hkLAB) on neuroblastoma cells and Caenorhabditis elegans.

Materials and methods: We pretreated heat-killed Lactobacillus fermentum CNU384 (hkCNU384), L. brevis CNU386 (hkCNU386), L. helveticus CNU395 (hkCNU395), and L. paracasei CNU396 (hkCNU396) at a concentration of 10⁹ CFU/mL to investigate their neuroprotective effects on glutamate-induced SH-SY5Y cells and their impact on the lifespan of 6-hydroxydopamine (6-OHDA)-induced C. elegans.

Results: Our findings indicate that hkCNU395 and hkCNU396 protected against glutamate-induced cell death …

Homologous And Heterologous Vaccination Regimens With Mrna And Rvsv Platforms Induce Potent Immune Responses Against Sftsv Glycoprotein, Tomaz B. Manzoni, Jonna B. Westover, Kendall A. Lundgreen, Philip D. Hicks, Raegan J. Petch, Jordan T. Ort, Drew Weissman, Steven H. Y. Fan, Scott E. Hensley, Norbert Pardi, Brian B. Gowen, Paul Bates

Animal, Dairy, and Veterinary Science Faculty Publications

Background: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a highly pathogenic bunyavirus with a high case-fatality ratio for which there is no approved vaccine. Studies have assessed different vaccine technologies. However, few studies have yet assessed the immunogenicity of heterologous prime-boost regimens. Methods: Here, we compare a lipid nanoparticle (LNP)-encapsulated nucleoside-modified mRNA-based vaccine encoding the SFTSV glycoproteins, Gn and Gc, to our recently described recombinant VSV SFTSV (rVSV-SFTSV) vaccine in single dose, homologous, and heterologous prime-boost regimens in mice. Results: We show that all regimens protect from pathogenic SFTSV challenge and elicit strong long-lasting antibody responses. Furthermore, strong cellular …

Effect Of Histidine Tag On The Enzymatic Activity Of Pseudolysin And Its Interaction With The Streptomyces Metalloprotease Inhibitor (Smpi) Protein, Adewale Adeboye Adewole

Electronic Theses and Dissertations

Pseudolysin (PLN) protein is a major virulent factor in Pseudomonas aeruginosa infection. PLN belongs to the family of zinc-dependent metalloproteases. It is naturally inhibited by a smaller protein called streptomyces metalloprotease inhibitor (SMPI), secreted by Streptomyces nigrescens. Previous and current studies demonstrate that the interaction between PLN and SMPI offers unique mechanistic insights for drug development against infections caused by P. aeruginosa. This study investigated the effect of N-terminal Histidine (His) tag on the enzymatic activity of PLN and its interaction with SMPI. His-tagged PLN and SMPI proteins were expressed in bacteria and purified by affinity chromatography …

Post-Drought Growth Recovery Of Tea (Camellia Sinensis) Under Different Techniques And Doses Of Biofertilizer Applications, Nabila Ragil Wiharti, Intan Ratna Dewi Anjarsari, Yudithia Maxiselly

Jurnal Kultivasi

Drought stress is a major limiting factor affecting the growth, yield, and quality of tea (Camellia sinensis). The present study aimed to analyze the success of post-drought growth recovery of tea in response to different techniques and doses of biofertilizer applications. Field experiments were conducted from January to August 2024 in the experimental garden of the Research Institute for Tea and Cinchona, Gambung Blok A8, Bandung. This work was arranged in a split-plot design, consisting of a main plot with two biofertilizer application techniques (foliar feeding and soil drenching) and subplots with four levels of biofertilizer dosage (control (B1), 15 …

Efficacy Of Lactobacillus Rhamnosus And Its Metabolites To Mitigate The Risk Of Foodborne Pathogens In Hydroponic Nutrient Solution, Esther Oginni, Robin A. Choudhury, Veerachandra Yemmireddy

School of Earth, Environmental, & Marine Sciences Faculty Publications

Hydroponic nutrient solution (HNS) has been established as an ideal conduit for pathogen contamination and proliferation. This study evaluated the efficacy of lactic acid bacteria and their metabolites in mitigating the risk of foodborne pathogens in HNS when compared to conventional chemical treatments. Hoagland’s HNS were prepared according to the manufacturer’s instructions and inoculated with Salmonella Typhimurium, Escherichia coli 0157:H7, and Listeria innocua at 105 CFU/mL cell concentration. These nutrient solutions were subjected to treatment with various concentrations of Lactobacillus rhamnosus live cells, a cell-free extract (CFE) of L. rhamnosus metabolites, sodium hypochlorite and peroxyacetic acid at 22 ± 1 …

Genetic And Environmental Factors Contributing To The Pathogenesis Of Vertebral Osteomyelitis Caused By Enterococcus Cecorum In Broiler Chicken, Ummu Syauqah Al Musyahadah, Andi Asnayanti, Anh Dang Trieu Do, Adnan Alrubaye

Poultry Science Faculty Publications and Presentations

Enterococcus cecorum (EC) is the primary cause of Vertebral Osteomyelitis (VO) in poultry, characterized by a hock-sitting posture with legs extended forward. The disease begins with intestinal colonization, where pathogenic strains of E. cecorum establish themselves in the gut within the first week of a bird's lifecycle and translocate to the bloodstream later, reaching the organs, which finally infect the birds' Free Thoracic Vertebrae (FTV). Several factors contribute to the initiation and progression of the disease. Environmental factors such as heat stress, high blood sugar levels, prior infections, overcrowding, and rapid growth conditions weaken immunity and stimulate the emergence of …

The Effect Of Feed And Water Provision Strategies On Broiler Breeder Pullet Performance And Welfare, Allison D. Weaver, Lisa R. Bielke, Ramon D. Malheiros, Sara K. Orlowski, Allison N. Pullin

Poultry Science Faculty Publications and Presentations

Feed restriction is common in the broiler breeder industry to optimize health and reproduction. However, this practice has been associated with increased drinking behavior, leading to water spillage, higher litter moisture, and footpad lesions. Consequently, parts of the industry have adopted water restriction protocols. This study aimed to evaluate how different combinations of feed and water restriction affected drinking behavior, welfare, and performance indicators in broiler breeder pullets. At 1 day of age, 960 Cobb 500 FF pullets (Gallus gallus domesticus) were randomly allocated to one of four treatments: skip-a-day feeding with ad libitum water (SAD + ADLIB), every-day feeding …

Effects Of Oil Spill Response Agents On Scleractinan Corals Acropora Cervicornis And Porites Divaricata, Austin Blakeslee

All HCAS Student Capstones, Theses, and Dissertations

Coral reefs are biodiverse coastal ecosystems at risk of exposure to spilled oil; spills reaching shorelines can damage corals, mangroves, and many other species in nearshore shallow-water tropical environments. Efforts to remediate such spills have evolved over the years through the introduction of various chemical response agents that efficiently and effectively control the spread of oil. Chemical herding agents and dispersants, which can contain and control the oil spread, are promising additions to the spill response toolbox as potential alternatives to other response methods. These response agents are designed to be biodegradable and minimize potential harm to aquatic ecosystems. However, …

Temporomandibular Joint Osteoarthritis And Systemic Inflammation: A Cadaveric Study For Dental Insights, Nathan Kent Bs, David Patterson Bs, Alyssa Luu Bs, Samantha Pipkin Bs, Jason Ciccotelli Dpt, Phd, Jeremy James Dmd, Ms, Jessica Immonen Phd, Ms

The Journal of Integrative Physiology

The objective of this study was to determine if gross signs of cadaveric osteoarthritis (OA) have

a tendency to present systemically and if this presentation includes pathology found at the

temporomandibular joint (TMJ). Signs of OA were then correlated to gross alterations of the aorta and common carotid arteries seen with atherosclerotic cardiovascular disease. Ipsilateral synovial joints including the TMJ, knee joint, radiocarpal joint and trapeziometacarpal (TMC) joint were all assessed for signs of OA in 4 cadaveric donors (3 males; 72, 82 and 92 years-old / 1 female; 89 years-old) using a validated scale of disease severity. A Grade …

The Legal System For Influencers On Social Media Platforms: A Comparative Study, Ahmad Ibrahim Ballal, Najem Riyad Al-Rabadi, Ma’Moun Rashed Al-Huneiti

Middle East Journal of Communication Studies

This study explores the legal regulation of social media influencers through a comparative analysis between French and Jordanian legislation. The research problem lies in the absence of a clear and comprehensive legal framework regulating influencers' growing role in shaping public opinion. Using the comparative analytical method, the study examines relevant legal texts and regulatory approaches in both jurisdictions. The findings highlight significant differences, with France enacting a specific law to govern influencer activities, while Jordan still relies on general legal provisions. The study recommends drafting a dedicated Jordanian law that outlines influencers' legal responsibilities and balances freedom of expression with …

Representations Of The Other In Hamas's Media Discourse During The "Al-Aqsa Flood" Operation: A Rhetorical Analytical Study, Enad Mamdouh Al-Zaben, Sabah Al Harahsheh

Middle East Journal of Communication Studies

This study aimed to determine how the Hamas movement represented the "other" in its media discourse during the "Al-Aqsa Flood" operation. To achieve this goal, the study relied on the qualitative analytical approach, specifically rhetorical discourse analysis. The study sample included all Hamas's media spokesman speeches, between October 7, 2023, and January 1, 2024, numbering 11 speeches. The study results showed that Hamas's media discourse focused on portraying two main groups: "us" and "them." The "us" group included the Palestinian people in all their spectra. They were referred to using terms with positive connotations such as: "the steadfast," "the one …

Addressing Anxiety In The Pediatric Population: An Ot Perspective, Joshua D. Johnson, Mary Jan Greer, Wendy Tremaine, Justine Faghihifar

Summer 2025 OTD Capstone Symposium

This capstone develops and evaluates a trauma-informed training for RBTs and caregivers in outpatient ABA clinics serving children with ASD and anxiety. Grounded in MOHO, the seven-module program teaches trigger→support mapping, sensory strategies, visual tools, and brief co-regulation routines. Findings show improved knowledge and confidence, with recommendations to embed a fidelity rubric, booster sessions, and ready-to-use templates.

Examining Associations Between Street Vegetation And Child Firearm Violence At Microgeographic Locations In 3 Us Cities: A Case-Control Study, Zihan Lin, Gregory A. Wellenius, Jonathan Jay

Biological, Geological, and Environmental Faculty Publications

Background: Firearm violence is a leading cause of death among U.S. children under age 18. Urban vegetation, a vital component of the living environment, has been found to influence firearm violence, yet the relationship between different types of street vegetation and firearm violence with children as the victim at the microgeographic scale remains unclear.

Methods: We analyzed 2316 child firearm assault incidents from Los Angeles, New York City, and Philadelphia from 2015 to 2021. Using a spatial case-control approach, we matched assault locations with control locations within 1-km. For each location, we applied deep learning algorithms to Google Street View …

Preventing Injuries In Cdl Drivers: An Occupational Therapy Approach, Jerika Eumecha Arnsworth, Cassandra Nelson, Lee Ann Hoffman

Summer 2025 OTD Capstone Symposium

Commercial drivers are at heightened risk for musculoskeletal disorders (MSDs) due to prolonged sitting, inadequate ergonomic environments, and limited access to health education. This doctoral capstone project aimed to address these challenges through the development, implementation, and evaluation of a five-week educational program focused on improving ergonomic awareness and occupational health among CDL drivers. Grounded in the Person-Environment-Occupation (PEO) model, the program was implemented in collaboration with a small commercial logging company in Texas and featured weekly modules on hydration, preparatory movements, assistive technology, seating posture, and mental health.

A needs assessment revealed limited ergonomic knowledge among participants and a …

First Report Of Fibropapillomatosis And Critical Habitat Use In Green Sea Turtles In Curaçao, M. Tripepi, I. J. R. Van Veghel, A. D. Vreugdenhil, E. Brunelli

College of Life Sciences Faculty Papers

Fibropapillomatosis, a disease affecting green sea turtles (Chelonia mydas), has been documented in many regions of the world, including the Caribbean, but has not been scientifically reported in Curaçao until now. The present study utilized VHF telemetry in Caracas Bay, Curaçao, to track green sea turtles, both healthy and affected by fibropapillomatosis, with the objective of ascertaining their foraging locations. VHF telemetry proved to be a pivotal method for assessing habitat use in areas characterized by limited visibility and high boat traffic, conditions that present significant challenges for divers and snorkellers attempting to access the area. The results …

From Gametes To Genes: Genetic Quality Assessment Of Anuran Cryopreserved Spermatozoa And Derived Embryos, Trinity Isabella Kassuhn

Theses and Dissertations

Sperm cryopreservation is an important tool within the suite of assisted reproductive technologies (ART) as it enables long-term storage of genetically valuable gametes. However, knowledge of the diagnostic criteria for assessing sperm quality and the genetic consequences of using cryopreserved sperm in amphibians is limited. This gap has raised concerns in captive breeding and reintroduction programs regarding the potential impact that cryopreserved sperm and the derived embryos may have on a population. The thesis aims to investigate how sperm cryopreservation affects the genetic material in both sperm and resulting embryos, with implications for in situ and ex situ amphibian populations. …

Enumerating Escherichia Coli (E. Coli) Concentrations And Evaluating Optical Brighteners As A Potential Indicator Of Human Wastewater Contamination In La Nana Bayou, Banita Creek, And Their Tributaries, Nacogdoches, Texas, Swastika Acharya, Matthew Mcbroom, Yuhui Weng, Yanli Zhang

Electronic Theses and Dissertations

La Nana Bayou was added to the Texas 303(d) list in 2000 after it failed to meet water quality standards set by the Texas Commission on Environmental Quality (TCEQ) for primary contact recreation due to elevated Escherichia coli (E. coli) levels. E. coli is a bacterial species commonly found in the intestines of humans and warm-blooded animals. It serves as an indicator of fecal contamination in freshwater, though its presence alone cannot identify the contamination source. Optical brighteners (OBs), chemicals found in laundry detergents, cleaning agents, toilet papers, and clothing, are unique to human wastewater. This study aimed …

Transcription Fidelity In The Face Of Oxidative Damage, Eastilan Gyiwah Appiah

Theses and Dissertations

It is very important that the process of transcription is correctly carried out in the cell for the faithful expression of the genetic code. However, should mistakes happen, they can result in transcription errors which interfere with the protein-making machinery of the cell. This will result in accumulation of proteins and dysfunctional proteins that have the potential of causing diseases like Alzheimer’s. Oxidative damage caused by hydrogen peroxide is known to cause transcription errors in organisms like yeast. It is therefore important to investigate the extent to which hydrogen peroxide can damage cells.

Integrated Analysis Of Untargeted Liver, Serum Metabolome And Gut Microbiome Following Selenium Deficiency Diet In Selenoh Knockout And Wildtype Mice, Richard Eugene Baird

Theses and Dissertations

Selenoprotein H (SELENOH) is an oxidoreductase located in the nucleolus, and its biochemical levels decrease with selenium (Se) deficiency, as reported in mammalian research. This current investigation aimed to understand the impact of nutritional Se deficiency on gut microbial composition and metabolomic profiles. The Firmicutes/Bacteroidota (F/B) ratio, linked to various health conditions, was balanced for wildtype (WT) mice (1.06). In contrast, selenoh knockout (KO) mice associated with dietary Se deprivation had a significantly (p < 0.002) lower ratio (0.56), suggesting dysbiosis. It is believed that SELENOH levels decreased due to the Se deficient diet, and the selenoh KO mice exhibited alterations in microbial community composition and metabolite profiles, as supported by correlative analyses. With respect to the bacterial communities, significant differences between diets occurred for three taxa, including Akkemansia mucinphila, Bacteroides caecimuris, and Parabacteroides goldsteinii. Candida tropicalis, Malassezia restricta, and Cyberlindnera jadinii were three abundant fungal yeasts. Only Cyberlindnera jadinii exhibited significantly greater relative abundance between genotypes and was more abundant in the selenoh KO versus WT mice. Correlation analyses between bacterial and fungal taxa revealed both positive and negative relationships, but no specific trends were observed. The liver and serum metabolomic data contained similar amino acids, lipids, proteins, and carbohydrates. Using NMR spectroscopy, 112 liver metabolites were detected, while GC-TOF-MS analysis of the serum revealed the presence of 172, comprising both polar and nonpolar compounds. The metabolite levels varied significantly when analyzed using multivariate models such as PCA and PLS-DA (VIP scores), particularly for glucose, taurine, lactate, and UDP-glucose, which are associated with energy production and cellular health. Overall, the Se deficient diet had the most significant impact on the microbial community structure and the concentration of key metabolites within liver tissues and serum. Correlation analyses conducted across studies revealed significant linear relationships between metabolites and the microbiome. However, these differences were associated with bacterial and fungal taxa containing low relative abundances. Based on these findings, further investigation into the impact of SELENOH homeostasis on microbial and metabolite profiles should be conducted using selenoh KO mice under various nutritional Se dietary levels.

Influence Of Diverse Forage Environments On Honey Bee Colony Health, Angus Catchot Iii

Theses and Dissertations

Honey bees are important pollinators that face a multitude of stressors in their environment such as pathogens, parasite pressure, forage availability and pesticide exposure. This study examined how the makeup of different environments and the stressors related to those environments are affecting honey bee health in Mississippi. A field experiment was conducted during the first year of the study across four distinct landscapes ranging from intensively cultivated cropland to predominantly natural forage habitats to assess the impact of landscape availability on varying honey bee colony health parameters. Locations that had cultivated crops within foraging range produced more honey and brood …

Strategic Use Of Exogenous Feed Enzymes And Sodium Bisulfate To Optimize Broiler Production, Spencer Thomas Karn

Theses and Dissertations

Poultry producers must continually evaluate nutritional strategies to enhance broiler performance and sustainability. Chapter II assessed sodium bisulfate (SB) applied to litter, feed, and water across a 42-day grow-out. Applying SB to litter reduced pH, while 0.3% and 0.4% dietary SB improved feed conversion ratio (FCR). Water acidification with SB during the final week did not impair performance, supporting its use as a preharvest intervention. Chapter III evaluated phytase at 500, 1500, and 2000 FTU/kg, with or without protease, in reduced amino acid diets over 56 days. Birds fed a nutrient-adequate diet with 1500 FTU/kg phytase outperformed all reduced-amino acid …

Understanding Pollinator-Forb Interactions And Evaluating Trapping Methods For Pollinator Monitoring In Lawns Of The Southeastern United States, Sara Rose Kirby

Theses and Dissertations

Urban turfgrass landscapes are widespread across the southeastern United States but offer limited ecological value under conventional management. This thesis evaluates the pollinator support potential of ten forb species commonly found in low-maintenance lawns and assesses the effectiveness of two insect sampling methods, sweep netting and pan trapping. Over two years of field surveys, native forbs such as Symphyotrichum divaricatum and Claytonia virginica supported significantly greater insect abundance and diversity than introduced species. Network and modularity analyses revealed that native plants facilitated more specialized pollinator interactions, including those involving oligolectic bee species. In a complementary study, pan traps effectively sampled …

Impact Of Buffered Dry Vinegar And Fermented Onion Juice On The Quality And Shelf-Life Of Fresh Pork Breakfast Sausage, Emily M. Little

Theses and Dissertations

The demand for clean-label meat products has increased interest in natural alternatives to synthetic antioxidants and antimicrobials. This study evaluated the effects of buffered dry vinegar (BDV), fermented onion juice (FOJ), and their combination (CMB) on microbial stability and sensory quality of pork sausage during 9 days of refrigerated storage. Sausages were assigned to control, FOJ (0.5%), BDV (0.5%), or CMB (0.25% each) treatments. BDV and CMB significantly reduced aerobic and psychrotrophic counts by days 7-9, while yeast increased across all treatments over time, reaching ~7 Log CFU/g by day 9. Sensory analysis revealed lower oxidation and off-note intensity in …