Digital Commons Network^™

Interdisciplinary Beef - Forage Management Demonstrations, G D. Lacefield, W R. Burris, E D. Miksch, M Rasnake

IGC Proceedings (1977-2023)

An interdisciplinary project, designated as Comprehensive Cow-Calf Management Demonstrations, was initiated by a term of extension specialists to demonstrate recommended management practices for cow-calf producers. The team consisted of extension specialists in beef cattle, agronomy and veterinary science. Cooperators for comprehensive on-farm demonstrations were nominated by county extension agents for agriculture and local veterinary practitioners. Selected cooperators were using a minimum of recommended practices but exhibited a willingness to make suggested changes. Farms were inventoried initially for cattle number and condition, soil capability and fertility, and existing facilities. Farm plans were then developed that would permit utilization of available resources …

Evaluating Correlates Of Healthy Eating And Dietary Quality Among Older Adults: A Mixed Methods Approach To Development And Application Of A New Survey Instrument, Sara K. Rosenkranz, Donya Shamati, Anna Biggins, Alissa Mick, Christopher Acosta, Richard R. Rosenkranz

Kinesiology and Nutrition Sciences Faculty Research

Objectives: Community-dwelling older adults face unique challenges related to nutrition and health, but little is known about their barriers and facilitators for healthy eating behaviors. This study sought to develop and evaluate a new instrument to measure the capability, opportunity, and motivation for healthy eating behaviors (COM-HE) among community-dwelling older adults.

Design: A mixed methods approach was used to obtain qualitative and quantitative data. Participants were aged 65 years or older, community-dwelling, and English-speaking. Participants engaged in focus groups (n = 12) and pilot-testing (n = 81) to evaluate the COM-HE instrument. The Rapid Eating Assessment for Participants – Shortened …

10.20.2025 Ored Connect, Liz Williamson

ORED Newsletter

• OTC Director Candidate Forum
• ORED Small Grants Program RFP

Genome Editing Approaches To Harness Cytokinin–Salicylic Acid Crosstalk For Plant Protection, Muhammad Naseem, Khalid Muhammad

All Works

No abstract provided.

Goals Reconsidered: From Brute Prelusory States Of Affairs To The Lusory Complexity Of Sport, Francisco Javier López Frías

Kinesiology and Health Science Faculty Publications

In this paper, I examine Bernard Suits’ conceptualization of goals in his influential definition of gameplay, focusing on how his account falls short of capturing the complexity of sporting practices. Suits famously describes games as voluntary attempts to overcome unnecessary obstacles in achieving an identifiable state of affairs. By referring to this state of affairs as the prelusory goal of games, he frames gameplay as structured teleologically around this goal, converting it into the most fundamental element of his notion of gameplay. Although elegant and very intuitive, this conception of games raises tensions when applied to the complex, multilayered activities …

Insights, Opportunities, And Challenges Provided By Large Cell Atlases, Martin Hemberg, Federico Marini, Shila Ghazanfar, Ahmad Al Ajami, Najla Abassi, Benedict Anchang, Bérénice A Benayoun, Yue Cao, Ken Chen, Yesid Cuesta-Astroz, Zachary Debruine, Calliope A Dendrou, Iwijn De Vlaminck, Katharina Imkeller, Ilya Korsunsky, Alex R Lederer, Jessica Jingyi Li, Pieter Meysman, Clint L Miller, Kerry A Mullan, Uwe Ohler, Pratibha Panwar, Nikolaos Patikas, Jonas Schuck, Jacqueline H Y Siu, Timothy J Triche, Alex Tsankov, Sander W Van Der Laan, Masanao Yajima, Jean Yang, Fabio Zanini, Ivana Jelic

Faculty, Staff and Student Publications

The field of single-cell biology is growing rapidly, generating large amounts of data from a variety of species, disease conditions, tissues, and organs. Coordinated efforts such as CZI CELLxGENE, HuBMAP, Broad Institute Single Cell Portal, and DISCO allow researchers to access large volumes of curated datasets, including more than just scRNA-seq data. These resources have created an opportunity to build and expand the computational biology ecosystem to develop tools necessary for data reuse and for extracting novel biological insights. We highlight achievements made so far, areas where further development is needed, and specific challenges that need to be overcome.

Alphasperm Data, Seth J. Parks, Timothy G. Jenkins

ScholarsArchive Data

Data from a prospective cohort study of sperm DNA methylation, semen analysis, and DNA fragmentation in fertile and infertile men before and after treatment with a male fertility antioxidant supplement.

Correlates Of Professional Breastfeeding Perceived Role, Perceived Influence, And Confidence In Providing Lactation Support Among Registered Dietitians And Registered Nurses, Lauren M. Dinour, Melanie Shefchik, Andrea Uguna

Department of Nutrition and Food Studies Scholarship and Creative Works

Lactation support from health professionals is a crucial social determinant of health. Registered dietitians (RDs) and registered nurses (RNs) can play an important role in supporting breastfeeding, yet most have minimal formal lactation education and training. This study seeks to explore the correlates of professional breastfeeding perceived role, perceived influence, and confidence in providing lactation support among RDs and RNs. Using a cross-sectional design, a convenience sample of 111 credentialed providers completed an online survey assessing their breastfeeding-related training, experiences, knowledge, attitudes, and practices. We also examined perceptions of social support, intentions for future breastfeeding, professional roles, and confidence in …

Quantifying Single, Compound And Cascading Climate Extremes: Implications For Agricultural Resilience In California, Shahryar Fazli, Wenzhao Li, Rejoice Thomas, Surendra Maharjan, Mohammad Sina Jahangiri, Andre Daccache, Hesham Morgan, Mohamed Allali, Hesham El-Askary

Mathematics, Physics, and Computer Science Faculty Articles and Research

As climate change intensifies, extreme weather increasingly threatens California’s Central Valley (CV), a vital agricultural region exposed to rising risks from heatwaves (HW), droughts (DR), and compound extremes. These events disrupt crop productivity and broader processes like water demand, pest dynamics, and soil stability, posing systemic risks. This study examines the spatiotemporal dynamics of HW, coldwaves (CW), DR, excessive rainfall (ER), and their compound (e.g., HWDR) and cascading forms from 1951 to 2025, using NOAA nClimGrid-Daily data. We assessed trends in frequency, intensity, and duration over long-term (1951–2025) and mid-term (1981–2025) periods. Results show increasing HW and DR in the …

Milk Yield Performance Of Local Cows, Handling Practices, Physicochemical Properties, And Milk Production Constraints In Kucha District, Southern Ethiopia, Demissie Jorge, Seifu Birhanu, Yilkal Tadele

The Thai Journal of Veterinary Medicine

The study assessed milk production performance of local cows, milk handling practices, physicochemical properties, and milk production constraints in Kucha District, Southern Ethiopia. A purposive and random sampling technique was employed to select 138 respondents for the interview. For physicochemical property assessment, milk samples were collected from 90 interviewed households. Majorities (76.7%) of the respondents were males, and the average family size was 6.82±0.25. Mean daily milk yield (DMY) of 1.80±0.07, 1.94±0.06, and 1.52±0.09 liters was recorded for local cows, respectively, in early, mid, and late lactation stages. All respondents in the area use hand milking and wash their hands …

Extraction And Optimization Of Flavonoids From Elaeagnus Pungens Thunb And Its Potential Impact On Blood Plasma Cholesterol In A Mouse Model, Qunqing Lu, Haozhe Liu, Xinxin Zhang, Nourhan Nassar, Ibrar Khan, Haji Gul, Zaigui Wang

The Thai Journal of Veterinary Medicine

Elaeagnus pungens Thunb is a significant source of flavonoids with biological function. This study aims to develop orthogonal tests and alkali alcohol extraction methods for extracting flavonoids from leaves of E. pungens. The levels of factors in the orthogonal test were determined by pre-experiment, and the physiological efficacy was verified by the in vivo test. The results indicated that the optimal extraction yield was 4.07% when the solid-to-liquid ratio was 1:40, the concentration of ethanol was 60%, the extraction temperature was 80℃, and the extraction time was five h. The alkalialcohol extraction results demonstrated that the extraction yield of …

Mesenchymal Stem Cells As Cornerstones Of Regenerative Medicine: Mechanisms, Applications, And Future Perspectives, Hasan M. Agha, Mohammed A. Abdalqader, Jantson Hoh, Yacoub Wassouf, Khudhur Abbas Ahmed Al-Saadawi, Amjad Abdulhadi Mohammed, Muneer Mohammed Saleh Alsayadi

AUIQ Complementary Biological System

Regenerative medicine is a multidisciplinary domain focused on restoring, repairing, or replacing damaged tissues and organs by harnessing the body’s inherent healing abilities with sophisticated biological and technical techniques. Mesenchymal stem cells (MSCs) have become fundamental among many stem cell types because to their self-renewal, multipotency, paracrine signaling, and immunomodulatory capabilities. Their therapeutic adaptability has been shown in orthopedic, cardiovascular, neurological, dermatological, and immune-mediated illnesses, underscoring their clinical significance. Nonetheless, obstacles like donor heterogeneity, senescence during in vitro growth, restricted engraftment, and regulatory impediments persist in limiting repeatability and broad clinical use. Recent advancements are enhancing the capabilities of MSCs …

Evaluating The Pros And Cons Of Common Dental Polymers In Dentistry: Review, Ali Kadhim Wadday, Sukaina Tuama Ghafel, Lina M. Shaker

AUIQ Complementary Biological System

Dental polymers play a central role in modern dentistry, offering versatility in applications ranging from removable dentures and prosthetic frameworks to implant abutments and bioactive scaffolds. Common materials like PMMA, PEEK, and nylon are valued for their formability, cost-effectiveness, and aesthetic properties, while emerging biopolymers including chitosan and alginate offer promising biocompatible alternatives. However, both conventional and bio-based polymers exhibit limitations in terms of chemical stability, mechanical wear, and microbial adhesion. Recent advancements in polymer science have expanded the material palette through innovative biopolymer formulations and advanced surface engineering techniques such as antimicrobial coatings and nanocomposites, introducing novel solutions for …

Creating A Food Business Model: Case Study Of Traditional Drinks Made From Roselle, Millet, And Corn, Josephine Nyame

College of Agriculture, Environment and Nutrition Sciences Graduate Theses and Dissertations

This study aimed to develop a comprehensive process flow for establishing a small food business, using traditional drinks made from millet, roselle, and corn as a case study. The specific objectives were to (1) identify the steps involved in developing a food product, (2) perform a sensory evaluation of the developed drinks, and (3) develop a prototype business plan to commercialize the most preferred drink. This study adopted a case study approach of a food business in producing traditional beverages. Consumer preferences were evaluated through sensory evaluation of the formulated roselle, millet, and corn drink recipes. Consumer evaluation data were …

Intracellular Lactate Dynamics In Drosophila Neurons, Matthew S Price, Elham Rastegari, Richa Gupta, Katie Vo, Travis I Moore, Kartik Venkatachalam

Faculty, Staff and Student Publications

Rates of lactate production and consumption reflect the metabolic state of many cell types, including neurons. Here, we investigated the effects of nutrient deprivation on lactate dynamics in Drosophila neurons by leveraging the limiting effects of the unstirred layer, a diffusion barrier that forms around cells in culture. We found that neurons constitutively consume lactate when the availability of trehalose, a glucose disaccharide preferred by insects, is limited by the unstirred layer. Acute mechanical disruption of the unstirred layer reduced this reliance on lactate. Through kinetic modeling and experimental validation, we demonstrate that neuronal lactate consumption rates under unstirred layer …

Nucleotide Motif-Guided Selection Of Plasma Microrna Biomarkers In Trauma, Boyang Ren, Ruoxing Li, Chien-Yu Lin, Chanhee Park, Sheng Wang, Andrew O Suen, John Kessler, Shiming Yang, Rosemary Kozar, Lin Zou, Brittney Williams, Ziyi Li, Peter Hu, Wei Chao

Faculty, Staff and Student Publications

Trauma remains a leading cause of morbidity and mortality in part due to complex pathophysiological responses. Yet our abilities to predict these changes are limited. Plasma miRNAs have been proposed as DAMPs that drive immune response and organ injury. Here, we test a panel of plasma miRNAs-selected based on next-generation sequencing and nucleotide motifs identified via a machine-learning algorithm-for their abilities to predict subclinical pathophysiological injuries. We find marked and severity-dependent increases in the miRNA biomarkers following trauma, which are closely associated with various injury markers. AUROC indicates that these biomarkers possess strong diagnostic and predictive abilities in overall trauma …

Visualization Of Lysosomal Membrane Proteins By Cryo Electron Tomography, Bridget M Mcveigh, José J De Jesús-Pérez, Dirk H Siepe, Prerana Gogoi, Shrawan Kumar Mageswaran, Marian Kalocsay, Elaine M Mihelc, Vera Y Moiseenkova-Bell

Faculty, Staff and Student Publications

Lysosomes are essential organelles for cellular homeostasis and signaling, with dysfunction linked to neurological disorders, lysosomal storage diseases, and cancer. While proteomics has advanced our understanding of lysosomal composition, the structural characterization of lysosomal membrane proteins in their native environment remains a significant challenge. Here, we developed a cryo electron tomography workflow to visualize lysosomal membrane proteins within intact, native lysosomal membranes. We isolated endolysosomes by independently targeting two lysosomal membrane proteins, transient receptor potential mucolipin 1 and transmembrane protein 192, enriching organelles that exhibited the expected morphology and proteomic composition of the endolysosomal system. Sub-tomogram averaging enabled the structural …

Polysaccharide From Schisandra Chinensis Alleviates Ulcerative Colitis By Improving Intestinal Microbiota Abundance And Inhibiting The Tlr4/P65/Iκbα Pathway In Dextran Sodium Sulfate-Induced Mice, Caihong Wu, Yang Yang, Peng Liu, Bin Zhang, Hui Lu

The Thai Journal of Veterinary Medicine

Schisandra chinensis polysaccharides (SCP) are isolated from Schisandra chinensis with a mean molecular weight of 32.401 kDa. Previous studies have demonstrated that Schisandra chinensis extract effectively prevented colitis and modulated gut microbiota in dextran sulfate sodium (DSS)-induced mice. In this study, 3% DSS was used to induce acute ulcerative colitis in mice, and then the SCP with different doses (50 mg/kg, 100 mg/kg, 200 mg/kg) and 5- aminosalicylic acid (5-ASA) were used to treat mice by oral gavage for one week. Here, we report for the first time that SCP presents outstanding protective effects on colon damage induced by DSS …

Design Of A Snp Panel For Highly Accurate Paternity Identification In Eşme Sheep, Onur Yilmaz, İbrahi̇m Cemal, Orhan Karaca, Cengi̇z Elmaci, Nezi̇h Ata

Turkish Journal of Veterinary & Animal Sciences

SNP paternity testing panels offer high-resolution capabilities for determining livestock paternity, thereby enhancing the reliability of genetic verification and ensuring pedigree record accuracy. In this study, we aimed to develop a SNP-based paternity test panel utilizing a 50K SNP chip for paternity testing and pedigree verification in the Eşme sheep breed. Additionally, the study sought to validate the paternity test panel. Based on data from 26 rams and 524 lambs of the Eşme sheep breed, the results indicated that the 50K SNP chip used demonstrated a very high genotyping rate, with the majority of its 53,738 SNPs exhibiting significant polymorphism. …

Optimizing Conditions For Bacillus Subtilis Ectopic Gene Expression And Delivery Via Seed Treatment, Abeer Alnasrawi, Jiamel Li, Payal Sanadhya, J. Alejandro Rojas, Fiona L. Goggins

Entomology and Plant Pathology Faculty Publications and Presentations

Bacillus subtilis is applied as a biofertilizer, biocontrol agent, and probiotic in agriculture, and is also used for industrial synthesis of proteins and peptides. These applications can be combined by using B. subtilis to synthesize plant health-promoting peptides in culture or to deliver them to roots via seed treatments. To facilitate the use of B. subtilis as a cell factory, we tested different media, temperatures, and growth phases to optimize ectopic expression of a Plant Elicitor Peptide from soybean (GmPEP3) that enhances seedling growth. Our results indicate that temperature, culture media, and growth phase have interactive effects, and that 30 …

Investigation Of The Usability Of The Difference In Body Structure As A Breeding Method In Unselected Sheep Flocks (I-Fattening Performance- Slaughter And Carcass Characteristics), Mehmet Naci̇ Oktay, Bora Özarslan

Turkish Journal of Veterinary & Animal Sciences

This study investigated the development of a meat-type sheep strain by using the differences in body structure in Kangal Akkaraman an indigenous sheep breed of Türkiye. The control group consisted of 15 male lambs born to ewes with dairy-type (DT) body structure and the experimental group consisted of 15 male lambs born to ewes with meat-type (MT) body structure. The sheep in the DT group were selected for their elegant, clear bony lines; long, thin neck; triangular body structure that widens from front to rear; and the leg length longer than the depth of the chest. In selecting sheep for …

Per- And Polyfluoroalkyl Substances (Pfas) In Urban Stormwater Runoff: Insights From A Roadside Rain Garden, Hadeer Saleh, Dibyendu Sarkar, Zhiming Zhang, Michel Boufadel, Rupali Datta

Michigan Tech Publications

Urban stormwater runoff is increasingly recognized as a critical but underexplored pathway for per- and polyfluoroalkyl substances (PFAS) to enter aquatic environments. This work investigated the occurrence and behavior of 40 PFAS compounds in stormwater runoff entering a roadside rain garden in Secaucus, New Jersey, during six storm events between August 2023 and July 2024. Total PFAS concentrations (Σ40 PFAS) ranged from 1437 to 1615 ng/L, with perfluorobutane sulfonate (PFBS, 239–303 ng/L) and perfluorohexanoic acid (PFHxA, 115–137 ng/L) consistently emerging as dominant species. Perfluorocarboxylic acids (PFCAs) and perfluorosulfonic acids (PFSAs) together accounted for over 70% of the total PFAS mass. …

Bacterial Identification, Antibiotic Susceptibility Testing (Ast), And Epstein-Barr Virus (Ebv) Testing To Increase Organ Transplantation Within A Western Regional Community, Vedarrius D. Williams

Doctoral Dissertations and Projects

Clinical transplantation demands have increased immensely in recent years and, along with increasing supply shortages, many barriers to successful organ donation arise from a lack of dedicated testing to rule out microbial or bloodborne infections. There are currently minimal, federal regulatory requirements for transplantation specimen testing. The purpose of this study is to explore how to increase the viability and success of transplantations in the Western regions of the United States using kidney, pancreas, and kidney/pancreas transplant data from University Medical Center Southern Nevada (UMCSN) transplant center. UMCSN transplant center and Donor Services Laboratory (DSL) in Las Vegas perform bacterial …

Protocol For Isolating Patient-Derived Ascites Cells And Extracellular Vesicles From Gastric Cancer Peritoneal Metastases, Yibo Fan, Jody V Vykoukal, Shumei Song, Melissa Pool Pizzi, Gengyi Zou, Katsuhiro Yoshimura, Jiankang Jin, Hiroyuki Katayama, George A Calin, Rebecca E Waters, Qiong Gan, Linghua Wang, Samir Hanash, Shilpa S Dhar, Jaffer A Ajani

Faculty, Staff and Student Publications

Peritoneal carcinomatosis (PC) cells and extracellular vesicles (EVs) from gastric cancer ascites are valuable for studying tumor-stroma interactions. Here, we present a protocol for isolating PC cells and EVs from patient ascites. We describe steps for PC cell culture, iodixanol-based EV purification, electron microscopy, nanoparticle tracking analysis, flow cytometry, and proteomic profiling. This protocol also includes TD-139 loading into exosomes for functional assays to evaluate their role in modulating the tumor microenvironment. For complete details on the use and execution of this protocol, please refer to Fan et al.

Equal Survival For Black Americans With Multiple Myeloma When Appropriately Matched To White Americans, David E Mery, Guido Tricot, Samer Al Hadidi, Yihao Zhan, Cody Ashby, Clyde Bailey, Eric R Siegel, Daisy V Alapat, Hongwei Xu, Sandra Mattox, Caroline Schinke, Maurizio Zangari, Sharmilan Thanendrarajan, Qing Yi, Robert Z Orlowski, Frits Van Rhee, John D Shaughnessy, Fenghuang Zhan

Faculty, Staff and Student Publications

No abstract provided.

Effect Of Water At Different Ph Levels Adjusted With Organic And Inorganic Acidifiers On The Growth Performance, Blood Biochemistry, Carcass Yield, And Meat Physicochemical Properties Of Broilers, Asad Ali Shah, Shahid Mehmood, Fazal Raziq, Muhammad Tahir Khan, Assad Ullah, Yasir Abbas, Syed Kaleem Ul Hassan, Sohail Ahmad, Smile Manzoor, Kashif Khan

Turkish Journal of Veterinary & Animal Sciences

This study evaluated the effects of varying water pH levels, adjusted with organic (formic acid) and inorganic (sodium hydrogen sulfate) acidifiers, on the growth performance, blood biochemistry, carcass yield, and meat quality of broiler chickens. The objective was to determine how these factors affect broiler health and production efficiency. In a 35-day feeding trial, 420 day-old chicks were randomly assigned to a 2 × 3 factorial design that tested two types of acidifiers (organic versus inorganic) at three pH levels (5.8, 6.8, and 7.8), with six replicates of 10 birds per treatment. Birds were provided with acidified water ad libitum …

Evaluating The Impact Of Starch Intake On Fecal Consistency Scores In Equines, Gülşah Kaya Karasu, Hidir Gümüş

Turkish Journal of Veterinary & Animal Sciences

Horses, as monogastric hindgut fermenters, primarily engage in microbial activity in the large intestine. Although starch is not inherently harmful, excessive intake can lead to digestive disturbances and health issues due to insufficient digestion in the small intestine, resulting in overflow into the large intestine. The purpose of the study was to evaluate the use of rapid fecal analysis, including fecal score, pH, and dry matter (DM) analysis, to understand the effects of high starch intake. The study was conducted in May 2023 at a private sport horse farm in İzmir, Türkiye and involved 24 sport horses aged 4 to …

Development And Validation Of A High-Performance Liquid Chromatography Method For The Simultaneous Determination Of Selected Varroacidal Active Substances In Bee Health Products, Onur Demi̇r

Turkish Journal of Veterinary & Animal Sciences

The presence of substandard and counterfeit veterinary medicinal products represents a significant concern from a One Health perspective, given the potential consequences for human and animal health, as well as the environment. In this study, an isocratic flow liquid chromatography method was developed for the simultaneous determination of thymol, coumaphos, amitraz, tau-fluvalinate, and flumethrin in bee health products used for varroosis control. The chromatographic separation was conducted at a wavelength of 276 nm, utilizing an RP18e column and an acetonitrile:water mobile phase ratio of 75:25 (v/v), with a flow rate of 0.75 mL/min. The validation of the method was conducted …

Pilot Study: Integrating The Epic Stroke Narrator Into Inpatient Code Stroke Protocols To Improve Symptom Onset Identification And Reduce Intervention Times, Stacy L. Carter

Doctoral Dissertations and Projects

In-hospital strokes are often underrecognized, leading to treatment delays and poorer outcomes compared to community-onset strokes. This scholarly project evaluated whether integrating the Epic Stroke Narrator into inpatient code stroke protocols could improve documentation of last known well time (LKWT) and reduce time to intervention. A 6-week quasi-experimental pilot study guided by the Iowa Model of Evidence-Based Practice and Rogers’ diffusion of innovations theory was conducted on a neuroscience telemetry step-down unit at a comprehensive stroke center in Richmond, Virginia. Education on the Stroke Narrator was provided to bedside nurses and was followed by implementation and evaluation of narrator use …

Dietary Patterns For Weight And Glycemic Management In Persons With Type 1 Diabetes: A Meta-Analysis Of Clinical Trials, Daria Igudesman, Laura M Nally, Alyssa A Grimshaw, Craig G Gunderson, Elizabeth G Considine, Laura M Jacobsen, Mustafa Tosur, Peter A Gottlieb, Irl B Hirsch, Lori M Laffel, Jennifer L Sherr, Chantal Mathieu, Richard E Pratley

Children’s Nutrition Research Center Staff Publications

Background: Medical nutrition therapy is fundamental for managing glycemia and weight in type 1 diabetes, yet dietary guidance specific to this population and relevant subgroups is lacking.

Purpose: We synthesized the interventional literature investigating diet patterns for glycemic and weight management in youth and adults with type 1 diabetes, with attention to interindividual variation that suggests the need for precision approaches. The protocol was prospectively registered (CRD42024519941).

Data sources: AMED, CINAHL, Cochrane Library, Ovid MEDLINE, Ovid Embase, Google Scholar, and Web of Science Core Collection were searched from January 2011 to June 2024.

Study selection: Clinical trials ≥4 weeks with …