Digital Commons Network^™

Quantitative Profiling Of Intratumor Immune Heterogeneity Identifies Loss Of Immune Diversity As A Hallmark Of Cancer Progression, Qiqi Lu, Jiangti Luo, Chia-Hao Tung, Xiaosheng Wang, Zhongming Zhao

Faculty, Staff and Student Publications

Immunological intratumor heterogeneity (ImTH) describes the variability in the types, spatial distribution, and functional states of immune cells within tumors. While evidence suggests that ImTH influences tumor progression and therapeutic response, few studies have provided a quantitative characterization of ImTH. Here, we present Scoring Immunological Intratumor Heterogeneity (ScImTH), a novel algorithm that quantifies ImTH by calculating the Shannon entropy of immune cell type proportions within the tumor microenvironment. Using bulk, single-cell, and spatial transcriptomic datasets, we show that reduced ScImTH scores are associated with unfavorable survival outcomes, tumor progression-related molecular and phenotypic features, immunosuppressive states, and resistance to immunotherapy across …

Target Consumers For Beef Jerky, Kynda R. Curtis, Makaylie Langford, Karin E. Allen, Ruby Ward

All Current Publications

This fact sheet describes the target market (consumer group) for packaged beef jerky, based on survey data collected from a Utah State University study in the fall of 2024. It includes details of the study and recommendations for beef jerky producers and retailers.

Exploring Biostimulant Efficacy For Onion Cultivation Under Limited Water And Nitrogen In Utah, Prakriti Nepal, Dan Drost, Youping Sun, Milena Oliveira

All Current Publications

This fact sheet provides information on using biostimulants to address the challenges of growing onions in Utah's semi-arid climate. It covers the role of biostimulants, Utah State University Extension case studies in Utah, harvest, and provides suggestions to mitigate nitrogen and water stress in onion production.

Preliminary Survey Of Subtidal Crustaceans On Southeast Sapelo Island, Georgia, Elise C. Mirabella, Kali Holierhoek, Kristine N. White Ph.D.

Georgia Journal of Science

The subtidal zone is the area below the low tide line that supports a wide variety of organisms, including subtidal crustaceans, which are understudied on Sapelo Island, GA. To investigate the impact of wave action on biodiversity and develop baseline data, the biodiversity of subtidal crustaceans from two beaches was compared. It was hypothesized that the beach protected from wave action would have higher biodiversity than the beach exposed to wave action. To collect the crustaceans, three different sampling methods were used on each beach: D-net, beam trawl, and Yabbi pump. The beach exposed to wave action yielded higher biodiversity …

Corrigendum To “Yi-Qi-Xuan-Fei Formula Ameliorate Chronic Obstructive Pulmonary Disease By Remodeling Lung And Intestinal Florase In Rat Models” [J Food Drug Anal 33(3) (2025) 252-276]

Journal of Food and Drug Analysis

No abstract provided.

Economic Evaluation Of Ai-Assisted Technologies In Healthcare: A Systematic Review, Wen-Tung Wu, Yi-Wen Chao, Tsung-Kun Lin, Ching-Kuo Huang, Ping-Hsuan Hsieh

Journal of Food and Drug Analysis

Artificial intelligence (AI) technologies are increasingly integrated into healthcare, yet their economic value remains uncertain. Traditional economic evaluation methods may not adequately capture the unique features of AI, including dynamic model evolution, scalability, and broader societal impacts. This systematic review synthesized existing evidence on the cost-effectiveness of AI-based healthcare interventions and assessed the methodological rigor of published studies. A comprehensive search identified health economic evaluations of AI applications published between September 2019 and March 2025, following PRISMA and SWiM guidelines and registered in PROSPERO (CRD42025641230). Eligible studies were full economic evaluations comparing AI-based interventions with non-AI alternatives, and data were …

Combination Treatment Of Cordycepin And Radiation Induces Apoptosis Accompanied By Protective Autophagy In Tm3 Mouse Leydig Progenitor Cells, Yu-Yan Lan, Yi-Ping Lee, Wei-Ru Huang, Chun-Ying Yu, Lyh-Jyh Hao, Chun-Hung Lin, Bu-Miin Huang

Journal of Food and Drug Analysis

Leydig cells are anatomically located in the testicular interstitial tissue, and their main function is to produce and secrete testosterone and indirectly support spermatogenesis. We previously reported that the combination treatment of cordycepin and radiation can effectively induce Leydig tumor cell apoptosis through cell cycle arrest, caspase activation, endoplasmic reticulum (ER) stress, reactive oxygen species (ROS) accumulation, and DNA damage. However, there is still a lack of scientific evidence for the susceptibility of normal Leydig cells to the combination treatment. In the present study, mouse TM3 Leydig progenitor cells were used as a model to evaluate the effects and mechanisms …

Development Of An Untargeted Metabolomics Analytical Protocol For Fecal Samples By Liquid Chromatography–Mass Spectrometry, Tsai-Wei Ting, Chih-Ning Cheng, Chieh-Chang Chen, Ching-Hua Kuo

Journal of Food and Drug Analysis

Gut microbiota has recently gained attention for its role in regulating multiple host pathways and contributing to disease developments. Fecal metabolomics using liquid chromatography—mass spectrometry (LC—MS) offers a promising approach to study gut microbial metabolites; however, it remains technically challenging due to the complex, heterogeneous nature of fecal samples and the lack of standardized protocols. This study aimed to establish a robust and reproducible untargeted fecal metabolomics workflow. We systematically evaluated sample preparation parameters―including sample amount, extraction solvent, numbers of extraction, and sample-to-solvent ratio―and assessed method reproducibility. Additionally, we compared three LC—MS data acquisition workflows using 10 samples from inflammatory …

Dna Tetrahedron-Au Nps-Go For Enhanced Fluorescence Detection Of Serotonin, Zhang Yun, Wu Qiong, Gao Hui, Zhang Hua, Miao Xiang-Min

Journal of Food and Drug Analysis

Evidence had accumulated that serotonin (5-HT), a hormone and neurotransmitter, was not only involved in a variety of different physiological and central nervous system functions but also in the development and manifestation of psychiatric diseases. Here, DNA tetrahedron-Au NPs-GO for enhanced fluorescence sensor was designed for detecting serotonin. Au NPs-GO was used as a fluorescence quencher, while fluorophore-labeled DNA tetrahedron was used as a donor, resulting in fluorescence resonance energy transfer (FRET) from the fluorophore to Au NPs-GO (signal off). With the strong binding ability of aptamer to serotonin, S5 was released from Aptamer: S5 duplex, then reacted with the …

Carotenoids Identified From Slipper-Shaped Oyster Magallana Bilineata Powder Residue Exhibit Potent Biological Activities, Rhoda Mae C. Simora, Raymund B. Parcon, Andrea Roxanne J. Anas, Tatsufumi Okino

Journal of Food and Drug Analysis

Carotenoids are a diverse class of biologically active compounds that contribute significantly to human health, serving vital functions in nutrition and overall well-being. Magallana bilineata, a commercially important oyster species, yields a shelf-stable powder residue possessing bioactivities with unknown specific compounds. Carotenoids are key marine bioactive compounds, but their presence in oysters remains underexplored. The present study aimed to identify the bioactive compounds from oyster powder residue through mass spectrometry for optimum utilization and value creation as a biomedical resource. The study employed solvent extraction of oyster powder residue, followed by fractionation using octadecylsilyl (ODS) column chromatography, liquid chromatography-mass …

Selective Sodium-Glucose Cotransporter Two Inhibitor Empagliflozin Ameliorates Diabetic Cardiomyopathy By Activating The Ampk/Tfeb Signaling Pathway., Man-Chen Hsu, Ru-Wen Chang, Mu-Chun Wang, Chia-Hui Chen, Wen-Hua Chen, Tzong-Shyuan Lee, Chih-Hsien Wang

Journal of Food and Drug Analysis

The highly selective SGLT2 inhibitor (SGLT2i) is reported to have beneficial effects on diabetic cardiac hypertrophy; however, the molecular mechanisms underlying the cardioprotection of SGLT2i are not fully understood. In this study, we investigated the impact of the SGLT2 Inhibitor empagliflozin (EMPA) on diabetic hearts and its regulatory mechanisms in high-fat-diet (HFD)- and streptozotocin (STZ)-treated rats. Male rats orally administered HFD/STZ treatment for eight weeks, with or without EMPA (10 mg/kg), were used as our in vivo model. Hematoxylin and eosin (H&E) staining was used for histological examination. Western blot analysis and immunohistochemistry were used to analyze the expression of …

Targeting Chemical Carcinogenesis: Α-Pinene And D-Limonene As Chemopreventive Agents Against Nnk/ Tcdd- And Urethane-Induced Lung Tumorigenesis, Kuo-Ching Huang, Vui-Hyen Chew, Yu-Ying Chen, Huey-Jen Su, Hao-Ting Chang, Rong‑Jane Chen, Ying‑Jan Wang

Journal of Food and Drug Analysis

Lung cancer is the leading cause of cancer-related deaths worldwide, driven by carcinogens such as tobacco-derived nitrosamines, dioxins, and urethane. This study evaluated the chemopreventive effects of two monoterpenes, α-pinene and D-limonene, using NNK/TCDD- and urethane-induced lung tumor models and A549 lung cancer cells. Both compounds significantly reduce tumor number and size, with enhanced effects when combined. Mechanistic studies revealed suppression of PRC1, β-catenin, and c-Myc, alongside activation of p53, Bax, and caspase-3, indicating inhibition of the PRC1—Wnt/β-catenin pathway and induction of apoptosis. These findings suggest α-pinene and D-limonene as safe, promising agents for lung cancer chemoprevention.

Fish Oil Rich In Ω-3 Fatty Acid Alleviates Long-Term High Fructose Consumption-Induced Fatty Liver And Kidney Dysfunction In Rats, Shing-Hwa Liu, Ruoh-Wenn Hung, Ting-Yu Chang, Meng-Tsan Chiang

Journal of Food and Drug Analysis

Excessive fructose intake leads to fatty liver and kidney dysfunction, with associated inflammation and metabolic disturbances. This study evaluated the effects of fish oil rich in ω-3 fatty acid on these conditions in rats fed a high- fructose diet. Male Sprague Dawley rats were divided into three groups: normal diet, high-fructose (HF) diet, and high- fructose diet with 5% fish oil supplementation. The experiment lasted for 21 weeks. Fish oil supplementation significantly reduced blood levels of total cholesterol, triglycerides, and advanced glycation end products (AGEs) in HF-fed rat. It also improved liver function markers and kidney function indicators. Mechanistically, fish …

Effects Of Chamomile And L-Theanine Beverage On Menstrual Pain, Menstrual Symptoms, Mood, And Sleep Quality In Young Women Experiencing Primary Dysmenorrhea: A Randomized, Double-Blind, Placebo-Controlled Study, Ziqing Soh, Soo Cing Tan, Tak Hiong Wong, Seok Tyug Tan, Seok Shin Tan, Chin Xuan Tan

Journal of Food and Drug Analysis

Primary dysmenorrhea is one of the most common complaints among young women. This study aimed to evaluate the effects of chamomile and L-theanine beverage intake on menstrual symptoms, pain intensity, mood, and sleep quality in young adult females with primary dysmenorrhea, using a two-phase continuous study design. In the first phase, a cross-sectional study was conducted to assess the prevalence of primary dysmenorrhea. In the second phase, participants were randomly assigned to either the intervention group (n =15), which consumed chamomile and L- theanine beverage (CTT), or the control group (n =15), which consumed chamomile-flavored beverage (non-CTT). Beverages …

Cordycepin In Cancer Therapy: A Bibliometric Analysis And Review Of Mechanisms, Zhiwei Ouyang, Yufei Zhang, Jianghan Ning, Yayi Tu, Bin He

Journal of Food and Drug Analysis

Cordycepin (3’-deoxyadenosine), a major bioactive component derived from fungi of the genus Cordyceps, has garnered significant attention in recent years for its potent antitumor properties. Drawing on literature indexed in the Web of Science Core Collection from 2004 to 2025, this study employs bibliometric tools―specifically CiteSpace and VOSviewer―to systematically examine developmental trends, research hotspots, and emerging frontiers in the field of cordycepin-related cancer research. The analysis maps a shift in focus from early-stage pharmacological validation to more advanced investigations into molecular mechanisms, with particular emphasis on cell cycle regulation. Keyword burst analysis highlights bursts in terms such as “apoptosis,” “cell …

Distinguishing Avocado Oil Quality Based On Fatty Acid Profile Using Pca: A Review Of Influencing Factors And Research Gaps, David Fernando, Ali Ridho Arif Madja, Nur Azizah, Agustina Ari Murti Budi Hastuti, Abdul Rohman

Journal of Food and Drug Analysis

The quality of avocado oil is influenced by multiple factors, including cultivar, growing region, drying method, harvest season, fruit maturity, extraction technique, and storage conditions. This review aims to show how fatty acid profiles (FAP), combined with principal component analysis (PCA), can be used to characterize avocado oil based on various established factors. A total of 23 peer-reviewed articles were included, encompassing 143 data points. PCA was applied as an exploratory tool to reduce dimensionality and visualize patterns in the data. Among the evaluated variables, the fruit part emerged as the most influential determinant, allowing clear categorization of avocado oils …

Cv: Sarah Powers (Biology), Sarah Powers

Biology Department Faculty Curricula Vitae

No abstract provided.

Clerodendrum Kelli (Lamiaceae), A New Species From Carranglan, Nueva Ecija, Philippines, And Lectotypification Of Clerodendrum Mindorense, David Justin R. Ples, Jacek Patykowski, John Charles A. Altomonte, Adriene B. Tobias, Rene Alfred Anton Bustamante

Biology Faculty Publications

Clerodendrum kelli (Lamiaceae), a new species from the montane forests of Carranglan, Nueva Ecija, Philippines, is described and illustrated. The species shares morphological features with Clerodendrum quadriloculare and C. mindorense, but differs in inflorescence structure, corolla tube length, and calyx shape and color. A lectotype for C. mindorense is also designated. Preliminary conservation assessment places C. kelli in the Critically Endangered category (CR B1ab(iii)+2ab(iii)). This study contributes to Philippine plant biodiversity knowledge and informs conservation strategies.

Rapid Detection Of Aflatoxin B 1 Contamination Levels In Peanuts Based On Dual-Band Hyperspectral Imaging And Machine Learning, Lu Ren, Shuyue Shen, Wanrong Shi, Chuang Rui, Maofeng Dong, Tingting Li

Journal of Food Science and Biotechnology

[Objective ] This study aims to achieve rapid detection of AFB 1 contamination levels in peanuts through dual-band hyperspectral imaging and machine learning.[Method ] A peanut AFB 1 contamination level warning model was constructed via a dual-band hyperspectral imaging system consisting of visible near-infrared spectroscopy at 400~1 000 nm and short-wave near-infrared spectroscopy at 900~1 700 nm,combined with machine learning.Peanut samples with four contamination levels were collected,in which the mass fraction of AFB 1 was determined by ultra-high performance liquid chromatography-tandem mass spectrometry.The effects of four pretreatment methods [first derivative,second derivative,multiplicative scatter correction,and standard normal variate (SNV )],two characteristic wavelength …

Research Progress In The Regulation Of Intestinal Stem Cell Homeostasis By Dietary Patterns, Haichao Zhang, Qi Sun, Yumei Qin, Yanpei Gu, Jianzhong Han

Journal of Food Science and Biotechnology

Intestinal stem cells (ISCs ) located at the bottom of intestinal crypts are the key factors driving the repair and rapid renewal of the intestinal epithelium.Their proliferation and differentiation are finely regulated by the microenvironment of the niche.ISCs,as dietary nutrient sensors,can respond to different dietary signals to regulate intestinal homeostasis.The author systematically summarizes the main signaling pathways involved in the regulation of ISCs and reviews the effects and functioning mechanisms of dietary patterns such as high-fat diets,intermittent fasting,calorie restriction,and ketogenic diets on the homeostasis of ISCs,aiming to provide a theoretical basis for the development and application of dietary intervention strategies …

Therapeutic Mechanisms Of A Lilium Brownii -Plantago Asiatica L.Compound Solid Beverage Against Hyperuricemia, Feng Li, Jiawei Huang, Xiaowen Guo, Lulu Jiang, Hongyan Zhao, Qiang Li, Chenxiang Sun, Shuying Feng, Qianqian Wang

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the ameliorative effects of a Lilium brownii -Plantago asiatica L.compound solid beverage on hyperuricemia and to elucidate its potential mechanisms.[Method ] Network pharmacology was employed to screen the active constituents,potential targets,and related pathways.Key targets were identified based on a protein-protein interaction (PPI) network.Molecular docking was conducted between the key proteins and active constituents.An animal experiment was performed to validate the effects of the compound solid beverage on serum uric acid levels,renal function indices,activities of uric acid-producing enzymes,and the levels of renal urate transporters.[Result ] Network pharmacology analysis indicated that Lilium brownii and Plantago …

First_Page Settings Order Article Reprints Open Accessarticle Quantitative Detection Of Salmonella Typhimurium In Ground Chicken Using A Surface Plasmon Resonance (Spr) Biosensor, Sandhya Thapa, Fur-Chi Chen

Food and Animal Sciences Faculty Research

Regulatory agencies worldwide have implemented stringent measures to monitor and reduce Salmonella contamination in poultry products. Rapid quantitative detection methods enable producers to identify contamination early, implement corrective actions, and enhance food safety. This study aimed to develop and optimize a surface plasmon resonance (SPR) biosensor for the quantitative detection of Salmonella Typhimurium in ground chicken. The sensor surface was functionalized with a well-characterized monoclonal antibody specific to Salmonella flagellin, and an SPR workflow was established for quantitative analysis. Ground chicken samples were inoculated with four S. Typhimurium strains at contamination levels ranging from −0.5 to 3.5 Log CFU/g and …

Research Progress In Enrichment Of Trace Elements Zinc And Selenium In Pleurotus Spp., Jing Zhang, Mengmeng Xu, Liting Zhao, Cui Li, Lei Chen, Song Ming, Zhongyang Ding

Journal of Food Science and Biotechnology

Zinc and selenium are essential trace elements for maintaining human normal physiological function.Deficiency of zinc or selenium can result in immune dysfunction,chronic diseases such as cardiovascular diseases and hyperlipidemia,and even increase the risk of cancer.The human body cannot independently synthesize these trace elements,which can only be obtained from the daily diet.However,natural food sources have low content of zinc and selenium,which is insufficient to meet the bod y’s needs.Therefore,developing fortified foods with trace nutrients is an important approach to meeting residents ’ requirements.Edible fungi serve as natural carriers for enriching trace elements and ideal dietary supplements.Pleurotus spp.,as a typical example,not only …

Construction And Optimization Of An Efficient Tyrosol Biosynthesis System In Escherichia Coli, Yizhe Xiong, Shougang Wang, Zhiming Rao, Meijuan Xu

Journal of Food Science and Biotechnology

[Objective ] An efficient biosynthetic system was constructed in Escherichia coli to achieve high-yield production of tyramine and its key derivative tyrosol.[Method ] Through the analysis of protein expression,enzymatic properties,and whole-cell catalysis results,tyrosine decarboxylase (TDC ) derived from Enterococcus faecalis was selected as the key enzyme for tyramine synthesis.Subsequently,tyramine oxidase (TYO ) was introduced to construct the TDC-TYO pathway for tyrosol production.Meanwhile,the LAAD-PDC pathway for tyrosol synthesis was established.A dual-enzyme co-expression system was established,and the effects of tandem enzyme order,production pathways,and key enzyme sources on tyrosol yield were analyzed.The recombinant strain B 1,carrying the plasmid pET 28a-EfTDC-TYO,was obtained through whole-cell …

Effects Of Single And Mixed Fermentation On Flavor , Composition , And Antioxidant Activity Of Soybean Whey, Taotao Wei, Qian Chen, Xiangjie Tao, Bin Tang, Fusheng Ren, Ping Hu

Journal of Food Science and Biotechnology

[Objective ] This study aims to explore the impacts of single strain fermentation and mixed fermentation on the flavor,composition,and antioxidant activity of soybean whey.[Method ] Gas chromatography-ion mobility spectrometry (GC-IMS ) was coupled with E-nose and high-performance liquid chromatography (HPLC ) to a nalyze the flavor and function of soybean whey fermented by the single and mixed strains.[Result ] Compared with single-strain fermentation by Limosilactobacillus fermentum HJS- 1 or Kluyveromyces marxianus HJS- 2,mixed fermentation (Limosilactobacillus fermentum HJS- 1 + Kluyveromyces marxianus HJS- 2) effectively improved the levels of functional substances and antioxidant activity of soybean whey.Compared with non-fermented soybean whey,mixed …

Goat Milk Formula Powder Ameliorates Lipopolysaccharide-Induced Intestinal Inflammation, Yi Shan, Xiaoling Liu, Shumei Wang

Journal of Food Science and Biotechnology

[Objective ] This study aimed to investigate the effects of goat milk formula powder on lipopolysaccharide (LPS)-induced intestinal inflammation and barrier impairment in mice.[Method ] Histopathological observation,real-time quantitative PCR,and enzyme-linked immunosorbent assay were used to assess changes in intestinal inflammation levels and barrier function-related indicators in mice following intervention with goat milk formula powder.Additionally,the composition of the gut microbiota was analyzed to elucidate its potential mechanisms of action.[Result ] Goat milk formula powder alleviated intestinal histopathology and increased the number of goblet cells.Compared with the model group,the goat milk formula powder intervention reduced the mass fractions of pro-inflammatory cytokines TNF- …

Effectiveness Of Acupressure At Different Ear Acupoints On Insomnia: A Secondary Analysis Of A Randomized Controlled Trial, Jingyu Zhang, Giulia Castelli, Aomei Shen, Junxin Li, Jennifer Kawi, Hulin Wu, Paul Christo, Claudia M Campbell, Johannes Thrul, Constance M Johnson, Nada Lukkahatai

Faculty, Staff and Student Publications

Introduction: Insomnia is highly prevalent among older adults with chronic low back pain (cLBP), contributing to a cycle of heightened pain sensitivity, impaired physical function, and reduced quality of life. Auricular Point Acupressure (APA), a nonpharmacologic intervention for pain, has shown emerging evidence of improving sleep. We compared the effectiveness of targeted APA (T-APA) versus non-targeted APA (NT-APA) on insomnia in older adults with cLBP.

Methods: This secondary analysis used data from a parent RCT, including 99 adults aged 60 or older with cLBP and clinical insomnia (Insomnia Severity Index [ISI]>7). Participants received four weeks of T-APA or NT-APA. …

Mapping Climatic And Environmental Determinants Of Parasitic Helminth Prevalence In Southeast Asia, Angelica Avisado

UNLV Theses, Dissertations, Professional Papers, and Capstones

Climate change is reshaping environmental conditions across Southeast Asia (SEA) through rising temperatures, altered precipitation patterns, and increased droughts. These changes alter transmission dynamics of neglected tropical diseases (NTDs) by shifting spatial distribution, increasing larval development rates, faster pathogen growth development, and increasing transmission rates. NTDs are a group of preventable and treatable infections that disproportionately affect more than 1 billion people globally, specifically in tropical and subtropical regions. A subgroup of NTDs, parasitic helminth infections (PHIs) - particularly schistosomiasis, soil-transmitted helminths, and lymphatic filariasis remains a public health concern in the Southeast Asian region. PHIs and their ecological responses …

Acute And Chronic Effects Of Neuroinflammation On Hippocampus And Anterior Cingulate Cortex Network Dynamics, Lauren A. Crew

UNLV Theses, Dissertations, Professional Papers, and Capstones

Neuroinflammation has emerged as a critical modulator of cognitive function and neural circuit dynamics, yet the distinct contributions of acute and chronic neuroinflammation to brain function remain poorly defined. This dissertation examines how inflammation independently alters large-scale neural activity and behavior, focusing on interactions between the hippocampus (HPC) and anterior cingulate cortex (ACC), regions essential for memory and executive processes. Using in vivo electrophysiological techniques in a rodent model, this study compares the effects of short-term (acute) and prolonged (chronic) neuroinflammatory states, induced via Poly I:C, on neural oscillations, regional coherence, and spatial coding. Findings reveal that even transient inflammation …

Functional And Microbial Consequences Of Experimental Evolution Under Starvation In Drosophila Melanogaster, Kristal Marie Aguilar-Conger

UNLV Theses, Dissertations, Professional Papers, and Capstones

Previous studies in the Gibbs lab have shown that starvation resistant Drosophila melanogaster flies have increased lipid content, lower metabolic rates, and poor physiological health. Historically, many evolutionary studies end at the phenotypic level, leaving the genomic basis largely unresolved. Using Evolve and Resequence (E&R) study, we can map genome-wide allele-frequency trajectories under selection, linking phenotypic shifts to their genomic underpinnings. This study utilized the E&R approach to identify enriched biological processes and pathways underlying starvation adaptation in Drosophila melanogaster. Five replicate restart populations (RS A–E) selected for starvation resistance, along with a control (RC), were resequenced at Generations 1, …