Digital Commons Network^™

Investigating The Ability Of Presequences To Influence Mitochondrial Protein Import, Youmian Yan

WUSM Theses and Dissertations – All Programs

Mitochondria are essential organelles housing over 1000 proteins, 99% of which are nuclear-encoded and synthesized in the cytosol. Most mitochondrial proteins are targeted by a positively charged sequence called the presequence, which lacks a common sequence motif and is poorly conserved across evolution. Accumulating evidence suggests that presequences encode meaningful information that influences mitochondrial protein uptake. For instance, multiple stress response models propose that stress sensing proteins contain “weak” presequences which allows them to monitor mitochondrial health. However, there is a lack of systematic examination about how presequences influence import kinetics and what constitutes presequence strength. These gaps in knowledge …

Evaluating The Pulse Of Exercise Programs: Developing And Validating The Intervention Usability Scale For Exercise (Iuse) To Enhance Implementation And Adherence, Anne Inger Mørtvedt, Eva Ageberg, Steven Elmer, Kevin Trewartha, Erich Petushek

Michigan Tech Publications

Background: Adherence to exercise interventions is often suboptimal, despite numerous studies documenting barriers and facilitators. Usability may be a critical yet underexplored determinant of adherence. This study aimed to develop and assess the psychometric properties of the Intervention Usability Scale for Exercise (IUSE). Methods: Item generation and content validation involved cognitive interviews and feedback from eight exercise intervention stakeholders and ten target users from the general public. Subsequently, 526 target users from University, Qualtrics and Prolific participant panels assessed exercise programs through an online survey. Dimensionality was assessed using Principal Component Analysis (PCA), Exploratory Factor Analysis (EFA), and bifactor models. …

Kinesiology Department Chairs’ Perceptions Of Their Training And Preparation, Kason O’Neil

Electronic Theses and Dissertations

Department chairs are responsible for leading academic departments in higher education.  These individuals regularly make important decisions regarding such things as managing faculty and academic programming, overseeing budgets, and supporting student needs. Despite these responsibilities, many people become department chairs without receiving formal leadership training. This study explored how kinesiology department chairs across the United States viewed their preparation for the role and the importance of leadership training. A total of 111 department chairs completed an online survey about their background, previous training, and the importance of training for different administrative responsibilities. The results showed that most chairs had little …

The Willing And The Compelled: How Power And Place Shape Human-Bison Coexistence In Poland And The United States, Patrick Orville Kelly

All Graduate Theses and Dissertations, Fall 2023 to Present

Wildlife recovery programs depend not just on animals, but on how people feel about living with them. The degree to which people accept the disruptions wildlife brings to their lives, known as social tolerance, can determine whether a recovery program succeeds or fails, yet it remains understudied and underapplied in management plans. This dissertation studies how people in Poland and the western United States feel about living with the two living species of bison, the largest land animals in Europe and North America. In Poland, we surveyed 242 residents living at different distances from European bison habitat. Surprisingly, the most …

Toward Optimizing Fruit Production In Utah Soils: Apple Salt Tolerance Screening And Tart Cherry Fertility Management, Kaitlin Dabbs

All Graduate Theses and Dissertations, Fall 2023 to Present

Fruit production in Utah can be challenging due to local climate and soil conditions. Tart cherries and apples are major fruit crops in Utah, and improvements to these cropping systems would be beneficial for farmers and local consumers. Two experiments were conducted to evaluate desired improvements for these crops. The first study looked for salt tolerance in ten new apple rootstocks. Salty soils can be found throughout the state limiting the land suitable for apple production. When grown in salty soils apple trees often have reduced growth and production potential. A specialized drip irrigation system was constructed in a greenhouse …

Wildfire And Fuel Treatments In Utah: A Practical Guide And Outcomes From An Oak-Maple Ecosystem, Annie E. Prescott

All Graduate Theses and Dissertations, Fall 2023 to Present

Wildfire is a natural part of Utah’s landscapes, but as wildfires become more extreme and communities continue to expand into wildland areas, it is increasingly important to understand how wildfires behave and how their negative effects can be mitigated. One way to reduce wildfire risks and protect life, property, and ecosystem services is through fuel treatments — actions that reduce the amount and arrangement of flammable vegetation through mechanical removal, wildland fire, or other methods.

Information on wildfire behavior and fuel treatments is abundant but often scattered, ecosystem-specific and difficult to access. In addition, most research in the Western U.S. …

A Global High-Resolution Comprehensive Heat Indices Dataset From 1950 To 2024, Abdul Malik, Sateesh Masabathini, Mohsin Ahmed Shaikh, Qinqin Kong, Muhammad Usman, Dasari Hari Prasad, Ibrahim Hoteit

All Works

Heatwaves are becoming more intense and frequent as global temperatures rise, affecting vulnerable populations, particularly in low-income communities. Addressing the impacts of heatwaves requires high-resolution data to assess their influence on labour productivity, public health, and climate risk. We introduce the Comprehensive Heat Indices (CHI) dataset, a high-resolution (0.1° × 0.1°) hourly dataset from 1950 to 2024, derived from the ERA5 and ERA5-Land reanalyses. The CHI dataset encompasses thirteen heat stress indices, including wet-bulb temperature, universal thermal climate index, mean radiant temperature, wind chill, and lethal heat stress index (Ls). Thresholds for Ls are empirically linked to mortality, enabling the …

Infestation By Phorid Flies Disrupts Behavior And Immune Function In Honey Bees Monitored By Radio-Frequency Identification, Gloria Ruiz-Guzmán, Oliverio Delgado-Carrillo, Francisco J. Balvino-Olvera, María De Jesús Aguilar-Aguilar, Violeta Patiño-Conde, Paulo De Souza, Ulises Olivares-Pinto, Mauricio Quesada

Research outputs 2022 to 2026

Honey bees (Apis mellifera Linnaeus) are important pollinators in natural and agricultural ecosystems and play a key role in global food production. However, colonies are increasingly affected by multiple stressors, including emerging parasites, which can compromise their health and performance. Among these, phorid flies (Diptera: Phoridae) have been observed in association with weak or collapsing honey bee colonies in various regions in Mexico, yet their effects on honey bee behavior and immune function under field conditions remain poorly understood. In this study, we investigated the impact of a phorid fly infestation on the behavior, survival, and immune defense of A. …

Exploring Competitive Dynamics Between Pseudomonas Baetica And Salmonella In A Polymicrobial System, Braxton E.M. Stout

Electronic Theses and Dissertations

The global rise in antimicrobial resistance has catalyzed a greater focus on polymicrobial research aimed at characterizing microbial interactions and uncovering new therapeutic strategies. Though it has long been of interest, environmental soil-derived bacteria remain largely under-represented in research and thus uncharacterized in this field. This project aimed to identify soil microbes that display inhibitory mechanisms to potential nosocomial pathogens. Soil sample S1S3-2 displayed inhibitory phenotypes toward numerous bacteria and was identified as Pseudomonas baetica. In both planktonic and biofilm growth, P. baetica was able to inhibit Salmonella arizonae through a contact-dependent and temperature-dependent manner. Screening of a transposon insertion …

In-Hospital Mortality Patterns And Readmissions In Patients With Chronic Obstructive Pulmonary Disease: An Analysis Of The Role Of Pulmonary Hypertension, Saad Afzal Khan, Trishna Parikh, Adishwar Rao, Akriti Agrawal, Aarohi Parikh, Farah Kazzaz, Sarah Shin, Harry Karmouty-Quintana, Maulin Patel, Kha Dinh, Bela Patel, Bindu Akkanti

Faculty, Staff and Student Publications

Chronic obstructive pulmonary disease (COPD) may be complicated by pulmonary hypertension (PH). We aimed to understand the impact of PH on in-hospital mortality and quantify the 30-day readmission rate among patients with COPD. For this cross-sectional study, we used the Nationwide Readmissions Database from 2017-2020 to identify adults ≥18 years with COPD. Patients were stratified according to PH diagnosis. Baseline characteristics between groups were compared using the Pearson chi-square test and two-sample t-test. Predictors of in-hospital mortality were determined using multivariate logistic regression analysis adjusted for demographics and confounders. The 30-day readmission rate and prevalence of PH subgroups by baseline …

Green Waste Biochar And Plant Growth-Promoting Bacteria Enhance Tomato Growth Under Combined Nutrient Deficiency And Salinity Stress, Soumaya Tounsi-Hammami, Munawwar Ali Khan, Mahra Alqemzi, Salama Ali Almehairi, Aneesa Rasheed Anwar

All Works

This study characterized a green-waste-derived biochar from date palms and ghaf trees and investigated its potential as a soil amendment with halotolerant Bacillus spp. to improve tomato seedling quality under dual stress of salinity and nutrient deficiency. Biochar was produced through pyrolysis at 450 °C and then characterized for yield, pH, electrical conductivity, proximate analysis, surface morphology, energy-dispersive X-ray spectroscopy, and heavy-metal content. Its effectiveness was tested both alone and in combination with a Bacillus sp. mix, using a completely randomized design with varying NPK fertilizer levels and saline irrigation. Tomato seedlings were evaluated 45 days after planting for various …

Harnessing Insect-Derived Oils For Enhanced Efficacy Of Plant-Based Repellents Against Disease-Transmitting Mosquitoes, John Bwire Ochola, Cynthia M. Mudalungu, Hosea O. Mokaya, Xavier Cheseto, Gilvian N. Onsomu, Subramanian Sevgan, Segenet Kelemu, Baldwyn Torto, Chrysantus M. Tanga

All Peer-Reviewed Publications

Mosquito-borne diseases such as malaria, dengue, chikungunya, Zika, and yellow fever pose a growing global health threat, worsened by resistance to synthetic pesticides. This study evaluated insect oils from Schistocerca gregaria, Ruspolia differens, and Macrotermes spp. as adjuvants in plant-based mosquito repellents. The formulations were tested for complete protection time (CPT) against Anopheles gambiae, Aedes aegypti, and Culex quinquefasciatus, and volatile organic compounds (VOCs) emitted from treated volunteers were analyzed. Incorporating insect oils doubled CPT compared to essential oils alone. The most effective blend of Macrotermes oil with Cymbopogon nardus (1:1) achieved CPTs of 3.50 h for An. gambiae, 2.56 …

Producer Organizations, Productivity And Sustainable Intensification Practices In Oil Palm Production, Martin Paul Tabe-Ojong, Abebayehu Girma Geffersa, Kibrom T. Sibhatu

All Peer-Reviewed Publications

Oil palm production is expanding in traditional farming and forested areas, especially in West and Central Africa around the Congo Forest Basin and mostly characterized by declining yields and yield gaps. Due to typically non-binding land access, independent producers tend to prioritize extensification over intensification, which has negative environmental consequences. Sustainable intensification (SI) of smallholder production is a guiding paradigm that aims to increase yields while minimizing negative environmental impacts. In this study, we examine the relationship between producer organizations (POs), sustainable intensification practices (SIPs), namely mulching and intercropping, and oil palm yields in Cameroon. We estimate actual and counterfactual …

Harnessing Digital Twin (Dt) Technology For Food Security And Climate Resilience In Sub-Saharan Africa (Ssa), Henri E.Z. Tonnang, Francis Chianu, Siyabusa Mkuhlani, Francis Muthoni, John Michael Humphries Choptiany, Franck B.N. Tonle, Bonoukpoe M. Sokame, Mercy Lung’Aho

All Peer-Reviewed Publications

Sub-Saharan Africa (SSA) faces chronic food insecurity despite possessing approximately 60% of the world’s uncultivated arable land. Field trials generate evidence but are costly and insufficiently scaled to address accelerating climate and demographic pressures. Digital twin (DT) technology, defined as the continuous, bidirectional virtual replication of physical systems using real-time data, supports monitoring, modelling, and optimisation of agrifood systems. To our knowledge, however, no published synthesis has examined DT agriculture research through the lens of SSA food systems or smallholder farming realities. A PRISMA-compliant systematic review was conducted across bibliographic databases using a pre-defined Boolean search and adapted PICOS eligibility …

The Other Visual Acuity, Paul B. Freeman Od

Optometric Clinical Practice

Editorial

A Comprehensive Review Of Current Trends And Future Prospects In Drug Resistance Of Leishmania Species, Sarah Kamal Naser

Maaen Journal for Medical Sciences

Leishmaniasis, which is an infectious tropical disease that is caused by Leishmania protozoa, continues to be a leading global public health problem. This disease is prevalent in about 89 countries, with over one billion individuals being at risk of infection. Current treatments have many limitations, and the efficacy of currently available leishmanial drugs has been undermined by the development of resistant strains of Leishmania. This review highlights the prevailing patterns regarding drug resistance in Leishmania and looks forward to future ways to deal with this problem. The genomic plasticity seen in Leishmania, which includes aneuploidy, gene amplification, chromosome rearrangement, and …

Radiological And Chemical Safety Assessment Of Drinking Water From Treatment Plants And Rivers In Kut City, Iraq, Ahmed A. Alswaty, Hadi D. Alattabi

Karbala International Journal of Modern Science

Climate change and increasing anthropogenic activities, particularly wastewater discharge into rivers, have raised pollution levels in the water sources of Kut City, necessitating an assessment of the radiological and chemical safety of drinking water at the city's treatment plants. Thirteen water samples were collected, comprising ten treated and three raw water samples from the source rivers. An HPGe detector was used to measure the radionuclides ²¹⁴Bi, ²¹⁴Pb, ²¹²Bi, ²¹²Pb, ⁴⁰K, and ¹³⁷Cs. All measured radionuclides were below the minimum detectable activity (MDA). ICP-OES analysis supported these findings, as U concentrations in most samples were …

Investigating The Role Of Gαf In Sperm Storage In The Spermathecae Of Female Drosophila Melanogaster, Aileen Liu

Holster Scholar Projects

Reproductive secretory cells are essential for fertility and have been implicated in diseases such as high-grade serous cancer, yet the molecular mechanisms governing their function remain poorly understood. Using genetic tools available in Drosophila melanogaster, we investigated whether Gαf contributes to reproductive secretory-cell function by examining its role in sperm storage. Gαf is highly expressed in these cells, suggesting a potential role in secretory cell function. Tissue-specific RNAi, trans-heterozygous mutants, and tissue-specific rescue were utilized to examine the role of Gαf in sperm storage. Gαf knockdown consistently reduced sperm storage, supporting a role for Gαf in maintaining normal sperm …

Risks Of Mercury Release From Disturbance Of Blue Carbon Ecosystems, Fan Zhang, Jingrou Hu, Ana Carolina Ruiz-Fernández, Christian J. Sanders, Xinxin Li, Ruotong Chen, Kunshan Bao, Bigyan Neupane, Aidah Baloch, Yanhui Zheng, Oscar Serrano

Research outputs 2022 to 2026

Blue carbon ecosystems (BCEs) function as dual repositories of organic carbon and legacy contaminants. While BCEs are recognized carbon sinks, their role as mercury (Hg) sinks and the risk of their conversion into sources due to human and climatic disturbances remain unexplored. This review synthesizes current knowledge on the drivers and risks of contaminant remobilization from threatened BCEs, with a primary focus on mercury. We explore how natural and human disturbances influence Hg storage, release, and bioavailability, while evaluating the risks posed to ecosystems and human health. Through a three-level meta-analysis, we show that physical disturbance remobilizes approximately 54% (95% …

Growing South Dakota (Fall 2026), College Of Agriculture, Food And, Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Support from donors helping set SDSU dairy graduates above the rest
[Page] 4 A decade of impact: Celebrating 10 years of cow-calf, swine education and research facilitates
[Page] 8 98-year-old donor keeps decades-long scholarship going in honor of late husband
[Page] 11 Looking back at Michele Dudash's tenure in academia
[Page] 12Giving students opportunities, experience to build their futures
[Page] 16 A Jackrabbit's journey in growing agricultural leadership
[Page] 18 Building South Dakota's next generation of rural veterinarians
[Page] 19 Conservation: A family affair
[Page] 20 South Dakota Water Resources Institute finds solutions to South Dakota's water challenges …

Exploring The Significance Of Kenya's Indigenous And Emerging Blue Foods For Sustainable Aquaculture Development. A Review, Jonathan Munguti, Mavindu Muthoka, Domitila Kyule, Esther Magondu, Kevin Obiero, Jimmy Mboya, Menaga Meenakshisundaram, Tanga M. Chrysantus, Gerald Kwikiriza, Francis K. Njonge

All Peer-Reviewed Publications

Kenya's aquaculture sector is at a pivotal stage, transitioning from a historical reliance on Nile tilapia and African catfish toward a more diversified production portfolio that integrates indigenous and emerging freshwater and marine species. This shift is increasingly recognized as essential to enhance climate resilience, food security, and ecosystem health. This systematic review synthesizes more than two decades (2000 to 31st January, 2026) of peer-reviewed literature to evaluate the biological suitability, culture performance, and socio-ecological roles of underutilized native taxa, including Ningu (Labeo victorianus), Jipe tilapia (Oreochromis jipe), freshwater prawns (Macrobrachium spp.), rabbitfish (Siganus sutor), marine-acclimatized Nile tilapia, mud crab …

Beyond Nutrients: Frass Fertilizer Legacy Effects For Sustainable Nematode Management In Potato–African Nightshade Rotation Systems, Emmanuel O. Anedo, Dennis Beesigamukama, Benson Mochoge, Nicholas K. Korir, Solveig Haukeland, Xavier Cheseto, Moses Nyongesa, Patrick Pwaipwai, Sevgan Subramanian, Abdou Tenkouano, Chrysantus M. Tanga

All Peer-Reviewed Publications

The utilization of insect frass fertilizer in crop production systems has recently gained significant global interest, but there has been limited evidence of its residual benefits in rotational cropping systems. The legacy effects of black soldier frass fertilizers (BSFFF), conventional organic fertilizer (SAFI), inorganic fertilizer (NPK), synthetic nematicide, and their combinations on potato cyst nematode (PCN) management under potato-African nightshade rotational farming systems were investigated. Eight treatments were evaluated: BSFFF, SAFI, chitin-fortified BSFFF (BSFFF+5chitin), NPK + nematicide, combination of BSFFF and NPK so that each supplies 50% of the nitrogen required by potato (50BSFFF+50NPK), combination of SAFI and NPK so …

Junior High School Students’ Understanding Of Pathogenic Microbes In The School Environment: A Cross-Grade Descriptive Study At Smp Alkhairaat 1 Palu, Feren Febrianti, I Nengah Kundera, Yulia Windarsih, Mohammad Jamhari, Mursito S. Bialangi, Abdul Ashari

Jurnal Pendidikan Sains

This study aimed to describe students’ understanding of pathogenic microbes and compare descriptive comprehension profiles across Grades VII, VIII, and IX at SMP Alkhairaat 1 Palu. A quantitative descriptive approach was employed involving 49 students selected through purposive sampling. Data were collected using an essay test as the primary instrument, supported by semi-structured interviews and observations of the school environment. The essay test assessed three indicators: conceptual knowledge of pathogenic microbes, understanding of disease transmission mechanisms, and understanding of clean and healthy practices for preventing the transmission of pathogenic microbes. Test data were analyzed descriptively using mean percentage scores, while …

The Impact Of Caffeine On Bumble Bee Hunger, Hallie Chapman, Becky Hansis-O'Neill, Aimee Dunlap Phd

Undergraduate Research Symposium

Bumble bees (Bombus impatiens) are crucial pollinators, responsible for pollinating multiple native plants and agricultural crops. Caffeine, which is classified as a stimulant, is present in some of these plant species at varying levels. Alterations in bumble bee cognition, including motivation, learning, and hunger, have been associated with caffeine intake. This study explored the impacts of caffeine on the bumble bee behavior on a biological level. While previous research has examined caffeine’s effects on cognition and learning, fewer studies have investigated its effects on hunger at the level of individual bees. Bumble bees, identified with numerically labeled tags, …

Enantioselectivity Of The Photochemical Continuous-Flow Isomerization Of Humulones, Gregory M. Giovine, Trevor Smith, Bruce Hamper

Undergraduate Research Symposium

Humulones, the primary natural product present in the historically significant hop flower, represent a largely unexplored candidate for structure-based pharmaceutical design.  Showing promising biological activity as an anti-bacterial and anti-inflammatory agent, humulones are of interest both for their bioactive properties and for their long-established tolerability and natural abundance.  Humulones undergo a characteristic isomerization reaction, where the six-membered substituted phloroglucinol ring contracts to a five-membered ring isohumulone, under a variety of conditions, including those enabled by brewing conditions (thermal, metal-catalyzed) and by exposure to UV light.  Optimization of the photocatalyzed isomerization of n-humulone to n-isohumulone in flow conditions resulted in a …

Sex-Related Differences In Fingerprint Minutiae Counts Among Secondary School Students In Sagamu, Ogun State, Nigeria, Olukayode Olushola Odubela, Osonuga Ifabunmi Oduyemi, Dr Kazeem Adeyinka Adeboyejo, Opeyemi Abel Bamgbose

AUIQ Complementary Biological System

Background: Dermatoglyphic minutiae are permanent epidermal ridge characteristics widely utilized in forensic identification and biometric science. Previous studies have demonstrated sex-related differences in fingerprint ridge morphology and ridge count, with females generally exhibiting higher ridge count than males. However, quantitative minutiae-based studies among Nigerian adolescents remain limited. This study evaluated sex-related and bilateral variations in fingerprint minutiae among secondary school students in Sagamu, Ogun State, Nigeria. Methods: This cross-sectional analytical study involved 300 apparently healthy students aged 14–18 years, recruited from an initial pool of 318 students assessed for eligibility across Batoro Community Grammar School, Remo Secondary School, and Remo …

Bacterial Biomass As A Versatile Platform For Functional Materials: Structural Insights And Sustainable Applications, Hasan M. Agha

AUIQ Complementary Biological System

Bacterial biomass has emerged as a versatile material with strong potential in environmental, material, and biotechnological applications, as well as broader uses such as bioplastics, packaging materials, and agricultural systems, owing to its rapid growth, controllable production, and surface chemistry enriched with carboxyl, hydroxyl, amino, phosphate, sulfate, and sulfhydryl groups. These features enable multiple interaction mechanisms, including electrostatic attraction, ion exchange, surface complexation, hydrogen bonding, and π –π interactions, supporting applications in pollutant removal, wastewater treatment, composite development, and bioenergy systems. Bacterial biomass can be sourced from natural environments, industrial fermentation, and waste streams, particularly activated sludge, making it compatible …

Exploratory Concurrent Associations Between K-Kyphometer And Flexicurve-Derived Kyphosis Measures (Kyphosis Index And Kyphosis Angle): Preliminary Study, Pimonpan Taweekarn, Kunavut Vannajak

Journal of Health Research

Background: Quantifying thoracic kyphosis is essential in musculoskeletal and rehabilitation practice. Although radiographic Cobb angle is commonly treated as a reference standard, repeated imaging is limited by cost and radiation exposure. Therefore, clinicians frequently use non-radiographic tools such as inclinometers and flexicurve rulers, which have shown acceptable reliability and variable levels of validity in different populations. The objective of this study was examine Pearson correlations between a patented K-Kyphometer (Utility Patent No. 23366) and two flexicurve-derived measures—Kyphosis Index (KI) and Kyphosis Angle (K angle).

Methods: In this cross-sectional correlation study (N=28), thoracic kyphosis was assessed using the K-Kyphometer and …

Biosensor Development In East Africa: A Systematic Review Of Technologies, Applications, Challenges, And Future Directions, Ally Mahadhy

Tanzania Journal of Science

Biosensors integrate biological recognition elements with signal transduction systems to generate quantitative or semi-quantitative analytical information and have applications across healthcare, food safety, agriculture, environmental monitoring, and wildlife health. Despite substantial global advances, biosensor development in East Africa remains poorly characterized. This systematic review, conducted according to PRISMA 2020 guidelines, evaluated published evidence from 1995 to 2025 within a predefined East African Community geographical scope. Eligibility was based on IUPAC-aligned operational criteria requiring an integrated biological recognition element, transduction mechanism, and quantitative or semi-quantitative signal output. Three locally developed biosensors met the criteria: an electrochemical nano-biosensor for schistosomiasis diagnosis in …

Scombrotoxin Poisoning In Marine Fish From Unguja Island, Zanzibar: Risks And Public Health Concerns, Khamis O. Haji, Prosper L. Mfilinge, Mwita J. Chacha

Tanzania Journal of Science

Histamine is the primary cause of Scombrotoxin Fish Poisoning (SFP) worldwide and poses health risks to populations dependent on fish and fish products. This study investigated histamine levels, awareness of SFP among health-care personnel and consumers, and fish preservation practices in Zanzibar. Three species, Auxis thazard, Sardinella gibbosa, and Lethrinus mahsena, were sampled on board (at sea during fishing), at landing sites, and in markets in Malindi and Chwaka. Histamine concentrations were measured using an amperometric enzymatic biosensor (BIOFISH-300 HIS, Biolan). Additionally, 270 consumers and 72 health-care personnel were surveyed using structured questionnaires, while post- harvest handling and preservation practices …