Digital Commons Network^™

A212: Experimental Study On The Kdl Physical Education Curriculum Promoting Preschoolers’ Gross Motor Development, Zhen Li, Xiao Niu, Normah Binti Jusoh, Yong Liu, Zhongji Hao

International Journal of Physical Activity and Health

Early childhood is a critical stage for the development of fundamental motor skills, with gross motor skills being the primary focus. Effective development of gross motor skills lays a foundation for learning complex movements during adolescence. Research confirmed that gross motor skills also directly affect Preschoolers’ perceptual, cognitive, and social adaptability development. This study explores the effectiveness of the "KDL Physical Education and Health Curriculum" in promoting gross motor skills development in preschoolers, aiming to enhance their overall health. From September to December 2024, 167 Preschoolers from primary, middle, and senior classes at Yijing Road Kindergarten in Chongqing Liangjiang New …

A213: Somatosensory Interactive Technology On Postural Balance In Children With Developmental Coordination Disorder: A Systematic Review, Changhui Peng, Lei Zhang, Xueping Wu, Xingyu Shi

International Journal of Physical Activity and Health

Developmental coordination disorder (DCD) is a neurodevelopmental disorder characterized by motor coordination impairments and motor learning difficulties. Compared with typically developing children, children with DCD often exhibit deficits in postural control, strength, and cardiorespiratory fitness. This study used a systematic review method to review the relevant evidence of video-game interventions in children with DCD, aiming to explore the mechanisms of somatosensory interaction technology on balance ability of children with DCD, and provide evidence-based recommendations for parents, teachers, and rehabilitation therapists. A PICO framework was constructed to systematically search Chinese and English literature on the intervention of video games on balance …

A208: Application Of Artificial Intelligence In Posture Correction, Jiaqi Wu

International Journal of Physical Activity and Health

In the modern fast-paced life, poor postures are extremely common, leading to many health problems such as neck pain and back pain. Posture correction is of great significance for maintaining physical health, and the rise of artificial intelligence (AI) technology has opened a new path for posture correction. Posture correction means using specific means to restore and maintain the correct body posture. This study aims to systematically review the application of AI in the field of posture correction, clarify its advantages, limitations, and future development trends. A comprehensive search was carried out in domestic and foreign authoritative databases for research …

A220: Work Memory Of Adhd Children: A Network Meta-Analysis Of The Effects Of Different Exercise Modalities, Bingjian Dai, Xuewen Wang, Shuxin Chen, Dongfei Liu

International Journal of Physical Activity and Health

Physical exercise, spanning from early childhood to adulthood, exerts positive influences on cognitive function. This study employed systematic review, network meta-analysis, and traditional meta-analysis to investigate the interventional effects of exercise on working memory in children with Attention Deficit Hyperactivity Disorder (ADHD). Relevant literature was retrieved from multiple databases. After screening and data extraction, study quality was assessed using the Cochrane Risk of Bias Tool. Meta-analysis, sensitivity analysis, and publication bias analysis were conducted using Review Manager 5.4 and Stata 18.0 to evaluate the impact of exercise on working memory in children with ADHD Among 1,296 screened studies, 49 met …

A218: Efficacy Of Kinesio Tape For Plantar Fasciitis: A Systematic Review And Meta-Analysis, Tingting Mo, Xinlong Zhang, Shilun Hou

International Journal of Physical Activity and Health

Plantar fasciitis is one of the most common causes of heel pain in adults and is a very common musculoskeletal system disorder. The incidence of plantar fasciitis is relatively high among sports enthusiasts, especially those involved in high-intensity, high-impact sports. Kinesio tape has the functions of relieving pain, correcting muscle function, and increasing body sensation. Currently, kinesio tape has been gradually applied to the treatment of the disease, but its effectiveness is unclear. The aim of this review is to assess how kinesio tape interventions affect pain and ankle and foot function in patients with plantar fasciitis. PubMed, Scopus, Web …

A226: The Application And Exploration Of An Ai Agent In Physical Education Teaching, Yali Si, Sumei Yu

International Journal of Physical Activity and Health

With the rapid development of artificial intelligence technology, AI-Agent, as a new type of educational technology tool, has gradually attracted widespread attention. As a system based on generative large-scale intelligent models, AI-Agent possesses core capabilities such as environmental perception, autonomous decision-making, and complex task execution. In the field of physical education, this technology holds significant application potential, particularly in personalized teaching, multimodal data processing, and enhancing teaching efficiency. However, current research on the application of AI agents in physical education is relatively scarce. This study explores the application of AI-Agent in physical education, analyzes its advantages, and investigates methods for …

A227: Eliminate Or Evolve: Digital Transformation Of Paris 2024 Olympics Under Olympic Ai Agenda, Tong Zheng

International Journal of Physical Activity and Health

The International Olympic Committee (IOC) has been promoting sports digitalization since the early 2000s, with the Olympic Agenda 2020 and the Olympic AI Agenda in 2024 providing strategic frameworks for this transformation. This paper examines how digital and AI technologies were applied at the 2024 Paris Olympics, the first Games guided by the exploration of impact on the Olympic movement and global sports landscape. Method: Using literature reviews and case studies from academic platforms like CNKI, WanFang, and Web of Science, this study analyzes digital transformation examples at the Paris 2024 Olympic Games. AI empowering the Olympics under the "Agenda" …

A228: Preservation And Dissemination Of Taijiquan Culture In Digital Perspective, Songqi Wang, Bin Yan

International Journal of Physical Activity and Health

One of China’s most iconic cultural traditions has increasingly gained: Taijiquned global recognition: Taijiquned. However, rapid digitalization has significantly changed young people's cultural preferences and participation habits, posing challenges to traditional methods of cultural preservation, which often fail to resonate with youth, creating a noticeable disconnect. This study examines the potential of digital communication—especially short videos, livestreaming, and social media—to preserve and disseminate Taijiquan culture through interactive, youth-oriented methods. Specifically, it empirically explores whether digital media strategies effectively improve younger generations’ cultural awareness, participation motivation, and cultural identity, aiming to provide practical insights for digital heritage protection. A randomized controlled …

A224: 15–17-Year-Old Student Swimmers Undergoing High-Altitude Training On Water Load Characteristics, Jiayi Lyu, Yuqi Wang, Xinyi Li

International Journal of Physical Activity and Health

With the continuous improvement of swimming competition level, high-altitude training, as a scientific and effective training method, has received attention from swimmers. Both professional teams and amateur sports schools give widespread attention to high-altitude training. The purpose of this study is to explore the characteristics of the three-week high-altitude training load of 15-17 male student swimmers and analyze whether it is effective for competition results. Ten male student swimmers (national level one or above) aged 15-17 years old were selected as the subjects and underwent a three-week high-altitude training at an altitude of 1900 meters. Method: The research method uses …

A207: Ai Era And Sports Equity: A Sociological Perspective, Jieqi Shi

International Journal of Physical Activity and Health

This study explores the impact of artificial intelligence (AI) on sports equity and inclusion, focusing on its use in training, officiating, and talent selection. AI includes technologies like AI-driven training tools and systems, such as VAR. While AI can enhance access to sports resources, it may also worsen existing inequalities. The goal is to examine how AI influences fairness in sports and promotes inclusion, particularly in university sports, while considering its effects on athlete development, decision-making, and fan engagement across social groups. A qualitative approach was used to examine AI in university sports. Participants include sports students, faculty, coaches, and …

A225: Applications Of Digitalization, Ai, And Big Data In Sports Science Research And Practice, Yixiao Zhang, Yue Xu, Zhihua Yin

International Journal of Physical Activity and Health

The rapid advancement of digitization, artificial intelligence (AI), and big data has revolutionized various industries. In the field of sports and exercise science, these technologies provide comprehensive insights into athlete performance, injury prevention, and personalized training (Smith & Johnson, 2022; Lee, 2021). However, the benefits and applications of these technologies in sports and exercise science remain understudied. Therefore, this study provides a systematic literature review of the impact and applications of digitization, AI, and big data in sports and exercise science. The search strategy followed the PEO framework and the PRESS checklist. Five databases were used for searching: ACM Digital …

A232: The Voice-Inclusive Physical Education Class: Primary School Physical Education Teaching Mode Under New Curriculum Standards, Qing Luan

International Journal of Physical Activity and Health

Current physical education classes in primary schools face a lot of challenges, such as the low student participation, a dull class environment, and a lack of students’ voice, which has a negative impact on teaching effect and students’ physical and mental health. This study employed the action research method to organize the physical education classes in primary school based on the New Curriculum Standards. The study encouraged primary school students to actively express their understanding and feelings about physical education activities and guided them to enthusiastically participate in the design of teaching content and activities to construct the “voice-inclusive” physical …

A234: The Challenge Of Physical Education Teachers In Primary And Secondary Schools, Ning Li, Jialin Liu

International Journal of Physical Activity and Health

Physical health in primary and secondary schools is the foundation for students' comprehensive development, and school sports are an important way to promote students' physical health. The implementation effect of school physical education largely depends on the professional competence and educational ability of physical education teachers. The problems faced by physical education teachers in the new era: the number and structure of physical education teachers; Challenges in teaching philosophy and abilities; The conflict between teaching pressure and the system; the disconnect between professional competence and training needs. Countermeasure: Optimize the construction of the physical education teacher team; Promote the reform …

A021: Analysis Of The Impact Of Ski Diagonal Stride Technique On Rehabilitation Treatment Effects, Xinming Tang

International Journal of Physical Activity and Health

The ski diagonal stride technique is a crucial component in cross-country skiing, engaging complex muscle coordination, enhancing cardiopulmonary function, and improving balance. In the realm of rehabilitation, traditional methods often face limitations. This study is dedicated to exploring how the physiological factors associated with this technique affect the rehabilitation treatment outcomes. By doing so, it aims to optimize rehabilitation strategies and ultimately enhance patients' physical capabilities and quality of life. Method: Eighty patients between the ages of 30 and 60, all in need of lower-limb rehabilitation, were evenly distributed into an experimental group and a control group. The experiment was …

A036: “The Champion Gene” Actn3 And Cold Adaptation: An Environmental Regression Model And Systematic Review, Mingrui Wang, Mi Zhou, Jianchun Weng, Longtianjiao Liu, Xiaoyu Jiang, Lijun Shi

International Journal of Physical Activity and Health

The ACTN3 R577X (rs1815739) genetic polymorphism is a significant research topic in sports genetics, with the XX genotype exhibiting a marked geographical gradient in different populations worldwide. The frequency of the XX genotype is notably higher in cold, high-latitude regions compared to tropical areas (e.g., Sweden 25%, Russia 14.2%, while Kenya 1%). These phenomena suggest that the ACTN3 R577X polymorphism may be closely related to cold adaptation. This study aims to analyze the evolutionary mechanisms, molecular regulation, and potential application of the ACTN3 R577X polymorphism in winter sports talent selection. Using the genomic database Ensemble and the WWF terrestrial ecoregion …

A053: Research On Adolescent Obesity Experience, Zhenfei Zhu

International Journal of Physical Activity and Health

The alarming prevalence of overweight and obesity among adolescents has emerged as a significant public health challenge. Since the World Health Organization (WHO) identified obesity as a global health priority in 2018, it has evolved from an individual concern to a medical condition requiring intervention, with profound implications for both individuals and society. Adolescent obesity is intricately linked to various aspects of health, including social, emotional, behavioral, and cultural dimensions. There is a notable lack of qualitative research on obesity experiences in China. Conducting qualitative studies to explore the life experiences, motivations, and physical activity patterns of students can deepen …

Understanding The Compatibility Of Fluoride-Based Radiopharmaceutical Reaction Solutions And Pdms, Mark Mc Veigh, Charles Frech, Mai Lin, Robert Ta, H Charles Manning, Leon M Bellan

Faculty, Staff and Student Publications

Microfluidic devices offer unique and exciting benefits when applied to radiopharmaceutical manufacturing, and these platforms are now starting to be integrated into commercial products. The field has strayed away from the use of polydimethylsiloxane (PDMS), the most common microfluidic device material, due to its suspected incompatibility with 18F, the most commonly used radionuclide. However, existing literature provides conflicting conclusions as to the existence and extent of this incompatibility. In this study, we use several analytical instruments to uncover the underlying interaction between fluoride and PDMS. SEM imaging and profilometry confirm the reactive relationship between the two materials and suggest that …

Autograft Enlargement After The Ross Procedure In Pediatric Patients: Somatic Growth Or Pathologic Dilatation?, Ioannis Zoupas, Alexander C Mills, Scott D Olson, Damien J Lapar

Faculty, Staff and Student Publications

Purpose of ReviewPulmonary autograft autotransplantation represents a popular surgical approach for pediatric patients requiring aortic valve replacement due to the potential for autograft enlargement to accommodate somatic growth. Nevertheless, autograft dilatation and the subsequent need for reintervention are quite common. Published data suggest that autograft enlargement may result from pathological passive remodeling rather than active somatic growth and vice versa. The present review serves to comprehensively evaluate available evidence related to the fate of the pulmonary autograft after the Ross procedure as it relates to the etiology, risk factors and patterns of autograft failure.ResultsStudies present conflicting results supporting both pathological …

Prevalence And Outcomes Of Her2-Low Versus Her2-0 Status In Patients With Metastatic Breast Cancer, Akshara Singareeka Raghavendra, Diane D Liu, Senthil Damodaran, Sarah Pasyar, Yu Shen, Jason A Mouabbi, Carlos H Barcenas, Kelly K Hunt, Debu Tripathy

Faculty, Staff and Student Publications

Background: HER2-low breast cancer (HER2 immunohistochemical [IHC] score 1+, or IHC 2+ without HER2 gene amplification) is distinct from HER2-positive and HER2-0 breast cancer (IHC 0), with a differing prognosis and specific therapeutic options. The DESTINY-Breast04 trial demonstrated notable efficacy of the HER2 antibody-drug conjugate trastuzumab deruxtecan over standard chemotherapy in patients with metastatic breast cancer (MBC) defined as HER2-low. More recently, the DESTINY-Breast06 trial confirmed this benefit in hormone receptor-positive and HER2-ultralow (less than 1+, but with ≤10% of infiltrating cancer cells showing incomplete and faint/weak membrane staining) cases, prompting re-evaluation of HER2 diagnostic thresholds and treatment strategies.

Methods …

Diet-Responsive Genetic Determinants Of Intestinal Colonization In The Yeast Candida Albicans, Musfirat Shubaita, Mazen Oneissi, Elena Lindemann-Pérez, Cecilia Fadhel Alvarez, Anne-Marie Krachler, Diana M Proctor, J Christian Pérez

Faculty, Staff and Student Publications

Dietary components influence microbial composition in the digestive tract. Although often viewed as energy sources, dietary components are likely to shape microbial determinants of intestinal colonization beyond metabolism. Here, we report that a dietary long-chain fatty acid enhances the yeast Candida albicans colonization of the murine gut partly by eliciting modifications to the fungal cell surface. Mice fed an oleic acid-rich diet were readily colonized by C. albicans and exhibited higher fungal load in feces compared with rodents fed an isocaloric control diet. Surprisingly, β-oxidation, a catabolic process to break down fatty acids for energy production, was dispensable for C. …

Feeding Performance And Survival Of Louisiana Poeciliid Fishes Exposed To Residential Wastewater Effluent, Jonathan Chapman

Masters Theses

Mosquitoes are a primary transmitter of vector-borne disease, and their larvae can thrive within shallow pools of stagnant water. Rainwater drainage ditches are a common sight in many communities, and in Louisiana, they are dually used as outlets for onsite residential wastewater discharge in neighborhoods disassociated from municipal sewage treatment. However, when discharge systems malfunction, the water becomes uninhabitable to mosquito predators, allowing mosquitoes to thrive. This research examines the predation performance of mosquitofish (Gambusia affinis), sailfin mollies (Poecilia latipinna), and least killifish (Heterandria formosa), the primary biological control agents utilized to combat mosquitoes within ditch communities. Individuals were captured …

The Food Ethics, Sustainability And Alternatives Course: A Mixed Assessment Of University Students’ Readiness For Change, Charles Feldman, Stephanie A. Navarro Silvera

Department of Nutrition and Food Studies Scholarship and Creative Works

Growing interest in food sustainability education aims to increase awareness of food distribution systems, environmental degradation, and the connectivity of sustainable and ethical food practices. However, recent scholarship has questioned whether such pedagogical efforts are meaningfully internalized by students or lead to sustained behavioral change. Prior studies document persistent gaps in students’ understanding of sustainability impacts and the limited effectiveness of existing instructional approaches in promoting transformative engagement. To address these concerns, the Food Ethics, Sustainability and Alternatives (FESA) course was implemented with 21 undergraduate and graduate students at Montclair State University (Montclair, NJ, USA). Course outcomes were evaluated using …

Direct Effects Of Capsaicin On Voltage-Dependent Calcium Channels Of Mammalian Skeletal Muscle, Dmytro Isaev, Tatiana Prytkova, Badarunnisa Mohamed, Mohamed Omar Mahgoub, Keun Hang Susan Yang, Murat Oz

All Works

Capsaicin, a naturally occurring polyphenol, is known to affect energy expenditure and muscle fatigue and modulate contractions in skeletal muscle. The L-type Ca²⁺ channels are known to be an important ion channel involved in the various muscle functions and the effect of capsaicin on the skeletal L-type Ca²⁺ channels is currently unknown. In this study, the effects of capsaicin and capsaicin analogs on depolarization-induced Ca²⁺ effluxes through L-type Ca²⁺ channels in transverse tubule membranes from rabbit skeletal muscle and L-type Ca²⁺ currents recorded using the whole-cell patch clamp technique in rat myotubes were examined. Capsaicin, …

Investigating Host Factors In Coronavirus Assembly And Egress, Surovi Binte Sharmin Mohona

Dissertations

Coronavirus (CoV) structural proteins congregate at the ER-Golgi intermediate compartments (ERGIC) prior to virus budding into the organelle lumen. Subsequent trafficking and fusion of virus-containing, ERGIC-derived vesicles then release nascent viruses from infected cells. Our findings in this dissertation illuminate mechanisms by which CoV structural proteins coalesce at the virus assembly and budding sites. The cytoplasmic tails of all CoV spike (S) glycoproteins contain variants of a consensus KxKxx coatomer-binding motif. Host coatomer complexes facilitate retrograde trafficking of client cargo proteins via recognition of such motifs. Utilizing reverse genetics methodologies, we introduced variations in the S coatomer motif in a …

Genome-Wide Gene By Sleepiness Interaction Analysis For Sleep Apnea, Pavithra Nagarajan, Nuzulul Kurniansyah, Jiwon Lee, Sina A Gharib, Yushan Xu, Yiyan Zhang, Brian Spitzer, Tariq Faquih, Hufeng Zhou, Eric Boerwinkle, Han Chen, Daniel J Gottlieb, Xiuqing Guo, Nancy L Heard-Costa, Bertha A Hidalgo, Daniel Levy, Peter Y Liu, Hao Mei, Rebecca Montalvan, Sutapa Mukherjee, Kari E North, George T O'Connor, Lyle J Palmer, Sanjay R Patel, Bruce M Psaty, Shaun M Purcell, Laura M Raffield, Stephen S Rich, Jerome I Rotter, Richa Saxena, Albert V Smith, Katie L Stone, Xiaofeng Zhu, Topmed Sleep Trait Working Group, Brian E Cade, Tamar Sofer, Susan Redline, Heming Wang

Faculty, Staff and Student Publications

Study objectives: Excessive daytime sleepiness (EDS), influenced by environmental and social-behavioral factors, is reported by a subset of patients with sleep apnea-a group that may be at elevated cardiovascular risk. However, it is unclear whether sleep apnea with and without EDS have distinct genetic underpinnings. In this study, we perform gene-by-EDS interaction analyses for apnea hypopnea index, a diagnostic marker of sleep apnea severity, to understand EDS's influence on its underlying genetic risk.

Methods: Discovery interaction analyses for common variants and gene-based rare variants were conducted respectively using multi-ethnic Trans-Omics for Precision Medicine (N = 11 619) data, followed by …

Workflow Improvements From Automated Large Vessel Occlusion Detection Algorithms Are Dependent On Care Team Engagement, Emmanuel C Ebirim, Ngoc Mai Le, Joseph N Samaha, Hussain Azeem, Ananya Iyyangar, Anjan N Ballekere, Saagar Dhanjani, Luca Giancardo, Eunyoung Lee, Sunil A Sheth

Faculty, Staff and Student Publications

Background: Automated machine learning (ML)-based large vessel occlusion (LVO) detection algorithms have been shown to improve in-hospital workflow metrics including door-to-groin time (DTG). The degree to which care team engagement and interaction are required for these benefits remains incompletely characterized.

Methods: This analysis was conducted as a pre-planned post-hoc analysis of a randomized prospective clinical trial. ML-based LVO detection software was implemented at four comprehensive stroke centers (CSCs) from January 1, 2021, to February 27, 2022. Patients were included if they underwent endovascular thrombectomy for LVO acute ischemic stroke. ML software utilization was quantified as the total number of active …

Mini-Hyper-Cvd Plus Venetoclax And Navitoclax For Relapsed/Refractory Acute Lymphoblastic Leukemia, Nicholas J Short, Hagop Kantarjian, Nitin Jain, Tapan M Kadia, Jayastu Senapati, Fadi G Haddad, Manuel M Maroun, Koji Sasaki, Lianchun Xiao, Xuelin Huang, Omer Karrar, Regina Abramova, Min Zhao, Rebecca Garris, Marina Konopleva, Farhad Ravandi, Elias Jabbour

Faculty, Staff and Student Publications

No abstract provided.

Therapeutic Responses In Patients With Advanced Nsclc Enrolled In Early-Phase Clinical Trials At Md Anderson, Jeong Uk Lim, Heather Y Lin, Lei Kang, Hung Le, Tin-Yun Tang, Stephane Champiat, Ecaterina Elena Dumbrava, Xiuning Le, Aung Naing, Sarina A Piha-Paul, Jordi Rodon Ahnert, Apostolia Maria Tsimberidou, Timothy A Yap, Siqing Fu, Funda Meric-Bernstam, David S Hong

Faculty, Staff and Student Publications

We evaluated clinical outcomes and safety profiles in patients with non-small cell lung cancer (NSCLC) treated in early-phase trials. A retrospective review of 546 NSCLC cases treated from January 2016 to December 2024 at The University of Texas MD Anderson Cancer Center was performed using the MD Anderson CHIMERA database. Patients were categorized into seven groups based on treatment regimen. The overall objective response rate (ORR) was 19.9%, and the highest ORRs were in the targeted combination (30.8%) and targeted monotherapy (29.7%) groups. Gene alteration–matched therapy, serum albumin level, metastatic burden, treatment group, and liver metastasis were independently associated with …

Structural Brain Differences Associated With Panic Disorder: An Enigma-Anxiety Working Group Mega-Analysis Of 4924 Individuals Worldwide, Laura K. M. Han, Willem B. Bruin, Janna Marie Bas-Hoogendam, Nynke A. Groenewold, Kevin Hilbert, Anderson M. Winkler, André Zugman, Takeshi Asami, Jacques P. Barber, Francesco Benedetti

Human Genetics Publications

Neuroanatomical findings on panic disorder (PD) are typically difficult to replicate, with inconsistent effects. These concerns prompted a paradigm shift towards large-scale collaborations, focused on harmonized data extraction and processing for robust examination of PD brain correlates. Hence, leveraging the largest-ever multi-site neuroimaging database on PD (Age: 10–66 years; global sites: 28), compiled by the ENIGMA-Anxiety Working Group, we report on cortical and subcortical differences in individuals with PD (N = 1146) versus healthy controls (HC: N = 3778). The analyses revealed lower thickness and smaller cortical surface area within fronto-temporo-parietal regions in PD (Cohen’s ds: −0.08–0.13), along with lower …

Contribution To The Chemotherapy Of Human Trypanosomiasis: Design, Synthesis, And Biological Evaluation Of Dimeric 2-Nitroimidazoles Against Trypanosoma Cruzi Amastigotes And Bloodstream Trypanosoma Brucei, Afonso Santine M.M. Velez, Otavio Augusto Chaves, Carlos Serpa, Fatma M. Salem, Bibo Li Ph.D., Bin Su Ph.D., Celio Geraldo Freire-De-Lima, Debra Decote-Ricardo, Marco Edilson Freire De Lima

Biological, Geological, and Environmental Faculty Publications

Effective and safe treatments for neglected tropical diseases caused by parasites, such as Chagas disease and sleeping sickness, remain lacking, posing a significant challenge for researchers worldwide. The rational design of dimeric compounds inspired solely by the pharmacophoric core of benznidazole (2-nitroimidazole) has proven to be a promising strategy for antiparasitic development. Thus, in the present work, it was increased the linker between the active units (2-nitroimidazole) to improve the interaction with TcNTR, facilitating the bioactivation of the longest dimers. Biological assays confirmed this, demonstrating that all compounds were active against replicative intracellular amastigotes of Trypanosoma cruzi (Tulahuen C2C4-LacZ). Notably, …