Digital Commons Network^™

Preclinical Fluorescence-Guided Imaging Leveraging Surrounding Sentinel Tumor Microenvironment Identifies High-Risk Premalignant Pancreatic Lesions, Shilpa Sharma, Xiaoxia Wen, Jianbo Wang, Beibei Huang, Denise A Hernandez, Cong-Dat Pham, Zhiwen Liu, Susanne Je-Han Lin, Aiko Yamaguchi, Dimitra K Georgiou, Ryan P Coll, H Charles Manning

Faculty, Staff and Student Publications

Purpose: Because surgery is the only potential cure for pancreatic cancer, high-risk premalignant pancreatic lesions often evade detection by palpation or white-light visualization, increasing the risk of recurrence. We asked whether near-infrared fluorescence imaging of tumor-associated inflammation could identify high-risk premalignant lesions, leveraging the tumor microenvironment as a sentinel of local disease and, thus, enhance surgery outcomes.

Experimental design: Fluorescence-guided surgery was performed on genetically engineered mice [Ptf1a-Cre; LSL-KrasG12D/+; Smad4flox/flox (KSC)] at discrete stages of disease progression, histologically confirmed high-risk, premalignant lesions in postnatal mice to locally advanced pancreatic tumors in adults, using the imaging agent V-1520, a translocator protein …

An Annotated Biobank Of Triple-Negative Breast Cancer Patient-Derived Xenografts Features Treatment-Naïve And Longitudinal Samples During Neoadjuvant Chemotherapy, Amanda L Rinkenbaugh, Yuan Qi, Shirong Cai, Jiansu Shao, Faiza Baameur Hancock, Sabrina L Jeter-Jones, Xiaomei Zhang, Emily Powell, Lei Huo, Rosanna Lau, Chunxiao Fu, Rebekah Gould, Petra Den Hollander, Elizabeth E Ravenberg, Jason B White, Gaiane M Rauch, Banu Arun, Clinton Yam, Alastair M Thompson, Gloria V Echeverria, Stacy L Moulder, W Fraser Symmans, Jeffrey T Chang, Helen Piwnica-Worms

Faculty, Staff and Student Publications

Triple-negative breast cancer (TNBC) that fails to respond to neoadjuvant chemotherapy (NACT) can be lethal. Developing effective strategies to eradicate chemoresistant disease requires experimental models that recapitulate the heterogeneity characteristic of TNBC. To that end, we established a biobank of 92 orthotopic patient-derived xenograft (PDX) models of TNBC from the tumors of 75 patients enrolled in the ARTEMIS clinical trial (NCT02276443), including 12 longitudinal sets generated from serial patient biopsies collected throughout NACT treatment and from metastatic disease. Models were established from both chemosensitive and chemoresistant tumors, and nearly 30% of the PDX models were capable of metastasizing …

Integrative Proteogenomics And Forward Genetics Reveal A Novel Mitotic Vulnerability In Triple-Negative Breast Cancer, Nicholas J Neill, Shankha Satpathy, Karsten Krug, Jitendra K Meena, Nivetha Ramesh Babu, Cheyenne Calderon, Desmon Reed, Marcus J Weber, Lacey E Dobrolecki, Alaina Lewis, Christina Sallas, Meenakshi Anurag, Kimberly R Holloway, Chen Huang, Suhas Vasaikar, Maria F Cardenas, Beom-Jun Kim, Doug W Chan, Shayan C Avanessian, Siddhartha Tyagi, Mayra Orellana, Sufeng Mao, Heyuan Li, Fade Gong, Sarah J Kurley, Kristen L Meerbrey, Calla M Olson, Amritha Nair, Tingting Sun, Hsiang-Ching Chung, Elizabeth A Bowling, Jarey H Wang, Pengju Zhang, Peng Xiao, Duxiao Yang, Fabio Stossi, Mei-Yin C Polley, Alexander B Saltzman, Filip Mundt, D R Mani, Michael A Gillette, Susan G Hilsenbeck, George Miles, Carolina Gutierrez, C Kent Osborne, Charles Y Lin, Nathanael S Gray, Jinpeng Sun, David A Wheeler, Charles M Perou, Anna Malovannaya, Michael T Lewis, Bing Zhang, Matthew J Ellis, Steven A Carr, Thomas F Westbrook

Faculty, Staff and Students Publications

Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer with few effective targeted therapies. Taxanes and other microtubule-targeting agents (MTAs) are frontline chemotherapies for TNBC; however, the molecular pathways that cause TNBC taxane sensitivity are largely unknown, preventing selection of taxane-responsive patients and development of more selective therapeutic strategies. In this study, we identified tumor-selective vulnerabilities in TNBC harboring inactivation of the tumor suppressor PTPN12 by integrating proteogenomic characterization and synthetic lethality screening. We discovered that PTPN12 inactivation drives mitotic defects through aberrant hyperactivation of the ubiquitin ligase complex APCFZR1, a critical regulator of the cell cycle. Consistent …

Orphan Nuclear Receptor 4a1 (Nr4a1) And Nr4a2 Are Endogenous Regulators Of Cd71 And Their Ligands Induce Ferroptosis In Breast Cancer, Arafat Rahman Oany, Srijana Upadhyay, Wai Ning Tiffany Tsui, Amanuel Hailemariam, Sarah Latka, John D Landua, Sandra D Scherer, Alana L Welm, Hugo Villanueva, Michael T Lewis, Stephen Safe

Faculty, Staff and Students Publications

Ferroptosis is an iron-dependent cell death pathway that involves multiple genes, including the transferrin receptor (TFRC/CD71), glutathione peroxidase 4 (GPX4) and cystine-glutamate antiporter (SLC7A11). This study is based on the hypothesis that orphan nuclear receptor 4A1 (NR4A1) and NR4A2 maintain low levels of ferroptosis in triple negative breast cancer (TNBC) cells and bis-indole derived (CDIM) compounds act as NR4A1/2 ligands that induce ferroptosis by enhancing CD71 expression. 1,1-Bis(3'-indolyl)-1-(3,5-disubstitutedphenyl)methane (DIM-3,5) analogs were investigated for their cytotoxicity and effects on NR4A1 and NR4A2 regulated genes and induction of ferroptosis. Several assays also determined enhanced lipoperoxidation, reactive oxygen species and malondialdehyde formation in …

Proteomic Perspectives On Kras-Driven Cancers And Emerging Therapeutic Approaches, Ramesh Karki, Ru Chen, Sheng Pan

Faculty, Staff and Student Publications

KRAS mutations are implicated in approximately 23% of all human malignancies, with particularly high prevalence in pancreatic ductal adenocarcinoma (PDAC) (~92%), colorectal cancer (CRC) (~49%), and non-small cell lung cancer (NSCLC) (~35%). The recent approval of the KRASG12C-specific inhibitors for NSCLC represents a pivotal advancement in KRAS-targeted therapy. Nevertheless, the emergence of intrinsic and acquired resistance to KRAS-targeted therapies poses a significant clinical obstacle to targeting KRAS, which necessitates a deeper understanding of the resistance mechanisms. Recent progress in proteomic studies has enabled comprehensive profiling of the proteomic alterations driven by KRAS mutations, offering valuable insights into the disrupted KRAS …

Ireland Blossoms Through Multiculturalism - Designing For Community Connection, Julia Kuczera, Shirley Casey, Hilary Kenna

SURE Journal: Science Undergraduate Research Experience Journal

No abstract provided.

The Impact Of Chronic Pain On Income And Employment, Jordan Vaarsi, Amber Jolly, Karim S. Ladha

SURE Journal: Science Undergraduate Research Experience Journal

No abstract provided.

Variation In Cycling Exercise Mechanical Efficiency In The Heritage Family Study, Angelo Tremblay, Claude Bouchard, Mark A. Sarzynski Facsm, James S. Skinner, Élisa Marin-Couture, Patrick Schrauwen, Guy Thibault, Marie-Eve Mathieu, Louis Pérusse

Faculty Publications

The objectives of the study were to assess the effects of cycling endurance exercise training and sex on cycling exercise mechanical efficiency (ME) and to explore its associations with body composition, glycemic profile, and skeletal muscle characteristics. Subjects were males (n = 371) and females (n = 465) of European (ED) and African descent (AD) of the HERITAGE Family Study. The measurements were repeated after a 20-week controlled endurance exercise program. A substudy involving 78 participants from 19 ED families was used to evaluate associations between skeletal muscle traits and variations in ME. An increase in ME of …

A Comprehensive Overview Of Chronic Wound Infections And Current Treatment Methods, Sarah Fakher, David J. Westenberg

Biological Sciences Faculty Research & Creative Works

Chronic wound infections pose significant healthcare challenges due to persistent biofilms, antibiotic resistance, and impaired healing pathways. These wounds are characterized by prolonged inflammation, microbial colonization, and disrupted tissue regeneration, leading to substantial morbidity and healthcare costs. This article reviews current knowledge on chronic wound types, their pathophysiology, and the mechanisms underlying biofilm formation to examine the latest advancements in antimicrobial-based strategies aimed at addressing these challenges. It highlights how diverse materials and technologies have been engineered to improve infection management, enhance tissue regeneration, and overcome the limitations of traditional treatments as well as advances that leverage innovations such as …

New Frontiers In Stand-Alone Digital Obesity Treatment, Delia West, Rebecca A. Krukowski

Faculty Publications

No abstract provided.

Phylogenetic Comparative Vocal Evolution, Casper W. Buchanan

Master's Theses

Vocal learning is a trait unique to humans, bats, cetaceans, primates, and birds (Jarvis & Nottebohm, 1997; McComb & Semple, 2005; Wilbrecht & Nottebohm, 2003). Although there has been extensive research on vocal learning in songbirds, parrots are an even closer proxy to human learning, as they are social, intelligent, and lifelong learners, with some having repertoires of over 300 sounds (Benedict et al., 2022; Péron et al., 2011; Thomsen et al., 2019; Toft & Wright, 2015). Elevated vocal learning can be attributed to the need for parrots to have strong communication skills through the sharing of information over foraging, …

Living Shoreline Prioritization And Hydrodynamic Habitat Suitability Models For Restoration In The Indian River Lagoon And Lake Worth Lagoon, Florida, Kelly M. Kibler, Melinda J. Donnelly, Vincent Encomio, Madison Giuntoli, Manisha Thenuwara, Brice Bennett, Laura Andrade Barron, Mariam Sonbol, Wynter Raine Seppala, Namritha Ramakrishnan, Jennifer Jordan Báez, Beth Orlando, Irene Arpayoglou, Melissa Meisenburg

Indian River Lagoon Shorelines

The spatial dataset: UCF_shorelinemodel_IRL_LWL.shp, was created with support from the Indian River Lagoon National Estuaries Program and Florida Sea Grant. These projects cumulatively led to creation of a living shoreline restoration prioritization model and hydrodynamic habitat suitability models for 1,550 km (963 miles) of estuarine shorelines in Indian River Lagoon and Lake Worth Lagoon.

The dataset presented herein includes:

1. shoreline data from >15,000 transects collected in the field,
2. shoreline wave climate data created through hydrodynamic modeling and frequency analysis of historic data,
3. categorization of potential risk for shoreline boat wake impact,
4. persistence of seagrass within 210 m of the shoreline, …

Age- And Step-Length-Dependent Alterations In Muscle Synergies And Joint Coordination During Unexpected Gait Termination, Woohyoung Jeon, Ashley Dalby, Xuanliang Neil Dong, Melinda Hermanns

Health & Kinesiology Faculty Publications and Presentations

Background: Falls in older adults often occur during unexpected gait termination, when rapid deceleration is required. Age-related declines in strength, proprioception, and coordination impair efficient distal-to-proximal braking, raising fall risk. Variable step lengths, ranging from “normal” steps to larger “big” steps used when hurrying, better reflect real-world walking demands and pose greater mechanical and neuromuscular challenges. This study investigated how age and step length affect muscle synergy complexity and joint coordination during unexpected gait stops.

Methods: Twelve healthy younger and twelve healthy older adults performed normal- and big-step stops in response to random visual cues during walking. Surface EMG from …

Hand Swelling And Other Non-Raynaud Phenomenon Symptoms As The Initial Presentation Of Systemic Sclerosis: Prevalence And Clinical Associations In Two Us Cohorts, Iqtidar Hanif, Shervin Assassi, Maureen D Mayes, Zsuzsanna H Mcmahan, Meng Zhang, Julio Charles, John M Vanburen, Jessica S Alvey, Kimia Ghaffari, Elana J Bernstein, Flavia V Castelino, Lorinda Chung, Luke Evnin, Tracy M Frech, Jessica K Gordon, Faye N Hant, Laura K Hummers, Dinesh Khanna, Kimberly S Lakin, Dorota Lebiedz-Odrobina, Yiming Luo, Ashima Makol, Jerry A Molitor, Duncan F Moore, Carrie Richardson, Nora Sandorfi, Ami A Shah, Ankoor Shah, Victoria K Shanmugam, Virginia D Steen, Elizabeth R Volkmann, Carleigh Zahn, Brian Skaug

Faculty, Staff and Student Publications

Objective: Raynaud phenomenon (RP) is often the initial clinical manifestation of systemic sclerosis (SSc), but some patients develop other manifestations first. To help elucidate the diversity of SSc presentation in its early stages, we describe the initial clinical manifestations and antinuclear antibody (ANA) profiles of patients in two early SSc cohorts.

Methods: All patient data in the Genetics vs Environment in Scleroderma Outcomes Study (GENISOS) and Collaborative National Quality and Efficacy Registry (CONQUER) cohorts were reviewed. Both studies enrolled patients within five years of the first non-RP symptom.

Results: In GENISOS and CONQUER, respectively, 194 (44.2%) of 439 and 292 …

3d Printed Microscopes As A Low-Cost Alternative To Conventional Light Microscopes, Aidan W. Donelan, Hunter N. Lemer, Sudeshna Roy, Nicholas C. Butzin

iLEARN Teaching Resources

Many K-12 schools function on limited budgets in their science departments, making it difficult to acquire, maintain, and update microscopes. While modern light microscopes are often complex, expensive, and require specialized training, they are based on simple principles of light. Microscopes enable us to observe objects too small for the naked eye and reveal intricate details. Introducing affordable, easy-to-use microscope adaptations early in education helps foster a deeper understanding of science and encourages young students to pursue STEM fields. Using 3D printing, we developed two innovative microscope models and two lesson plans. Lesson 1: Using a smartphone microscope to visualize …

Comparison Of Relative Calcium Bioavailability Based On Bone Ash And Apparent Ileal Ca Digestibility In Broiler Chickens, R. L. Drysdale, B. W. Parsons

Poultry Science Faculty Publications and Presentations

Two experiments were conducted to determine relative Ca bioavailability based on bone ash and apparent ileal digestibility (AID) of Ca in reagent-grade calcium carbonate (RCaCO3) and RCaCO3 + phytic acid in commercial broiler chickens. In Experiment 1, relative Ca bioavailability of RCaCO3 and RCaCO3 + phytic acid was determined in ad libitum-fed broiler chickens using the slope-ratio method. Dietary treatments were corn-soybean meal-based diets. Diet 1 was Ca-deficient (0.30 % Ca; 0.45 % non-phytate P), while diets 2 through 5 were the same as diet 1, except that 2 increasing levels of RCaCO3 or RCaCO3 + phytic acid were added …

Marine Environmental Protection : An Investigation Into Existing Global Laws Addressing Marine Plastic Pollution, Maryland De Castro

World Maritime University Dissertations

No abstract provided.

Assessing The Economic Impacts Of The Illegal, Unreported And Unregulated Fishing On The Namibian Coastal Communities : Challenges And Mitigation Strategies, Susana Kashinashilonga Angula

World Maritime University Dissertations

No abstract provided.

Biosecurity Investment Impacts On Highly Pathogenic Avian Influenza Control In United States Commercial Turkey Operations, Jada M. Thompson, Kelly Patyk, Victoria Fields, Matthew Branan, Amy Delgado, Kylie Roesler

Agricultural Economics and Agribusiness Faculty Publications and Presentations

Highly pathogenic avian influenza (HPAI) is an extremely transmissible virus that poses a significant threat to the U.S. turkey industry through contagion from migratory birds and results in whole-farm depopulation. To combat the disastrous economic impacts of HPAI, this study examined how biosecurity investments may have economic benefits to producers and the industry. Using data collected from a farm-focused survey of commercial turkey producers, the differences in biosecurity practices between farms affected by HPAI and unaffected farms in 2022 were analyzed. This evaluation aims to understand how effective biosecurity investments were towards reducing the susceptibility of a turkey farm to …

Genomic And Metabolic Network Characterization Of Fusarium Species, Hend Hussain Mueenuddin

Theses

Fusarium covers the wide genus of fungi with over 400 species that are key in agricultural and medical practice. The purpose of the current study was to perform a comprehensive genomic profiling of the 19 Fusarium species that were captured in the National Centre of Biotechnology Information (NCBI) database. The study was made to make a comparison of the data, based on the descriptive statistics of every species, with graphical representation images enabling clear demonstration of the numbers. The study indicated that the chromosomal numbers were in the range of 0-15, the gene count was roughly 11000 -19000, while that …

‘Earth System Engineers’ And The Cumulative Impact Of Organisms In Deep Time, Simon A. F. Darroch, Michelle M. Casey, Alison T. Cribb, Amanda E. Bates, Matthew E. Clapham, Dori L. Contreras, Matthew Craffey, Ivo A. P. Duijnstee, William Gearty, Nicholas J. Gotelli, Marcus J. Hamilton, Riley F. Hayes, Pincelli M. Hull, Daniel E. Ibarra, V. A. Korasidis, Jaemin Lee, Cindy V. Looy, Tyler R. Lyson, Benjamin Muddiman, Peter D. Roopnarine, Alex B. Shupinski, Felisa A. Smith, Alycia L. Stigall, Catalina P. Tomé, Katherine A. Turk, Amelia Villaseñor, Peter J. Wagner, Steve C. Wang, S. Kathleen Lyons

College of Life Sciences Faculty Papers

Understanding the role of humans as 'ecosystem engineers' requires a deep-time perspective rooted in evolutionary history and the fossil record. However, no conceptual framework exists for studying the rise of ecosystem engineering in deep time, requiring us to consider effects that fall outside the scope of traditional definitions. Here, we present a new framework applicable to both modern and ancient engineering-type effects. We propose a new term - 'Earth system engineering' - to describe biological processes that alter the structure and function of planetary spheres, and which combines core tenets of ecosystem engineering, niche construction, and legacy effects. We illustrate …

Developing Hydrophobic-Hydrophilic Protein Structures By 3d Food Printing Of Sorghum And Soy Protein Gels, Sorour Barekat, Ali Ubeyitogullari

Food Science Faculty Publications and Presentations

The goal of this study was to introduce the simultaneous use of hydrophobic-hydrophilic proteins bioinks for 3D food printing. Sorghum protein gel (SPG) at 25 % w/w and soy protein isolate gel (SPIG) at 11 % w/w were loaded into separate extruders. The effects of the first printed layer (SPG or SPIG), printing speed (10, 20 mm/s), and nozzle size (0.52, 0.64, 0.72 mm) on printability were evaluated. The textural properties, rheological characteristics, microstructure, crystallinities, and functional groups of the proteins were analyzed. Printing sorghum and soy proteins together helped balance essential amino acids, where lysine (94.7 %) and histidine …

Khsrp-Mediated Decay Of Axonally Localized Prenyl-Cdc42 Mrna Slows Nerve Regeneration, Matthew D. Zdradzinski, Lauren S. Vaughn, Samaneh Matoo, Kayleigh Trumbull, Terika P. Smith, Davis Noblitt, Courtney N. Buchanan, Ashley Loomis, Elizabeth Thames, Seung Joon Lee, Nora Perrone-Bizzozero, Qun Lu, Jessica M. Larsen, Jeffery L. Twiss

Faculty Publications

The small GTPase CDC42 promotes axon growth through actin filament polymerization and this growth is driven by axonal localization of the mRNA encoding the prenylated CDC42 isoform (Prenyl-Cdc42). Here, we show that axonal Prenyl-Cdc42 mRNA levels and the mRNA's translation are decreased by growth-inhibiting stimulation and increased by growth-promoting stimulation. In contrast, axonal RhoA mRNA transport and translation are increased by growth-inhibiting but unaffected by growth-promoting stimuli. Localized increase in KHSRP in response to growth inhibitory stimulation, through elevation of intracellular Ca2+, promotes decrease in axonal levels of Prenyl-Cdc42 mRNA. Distinct 3'UTR motifs regulate transport and axonal levels of Prenyl-Cdc42 …

Expanding Functionalities Of Immune Receptors Through Simultaneous Transfer Of Sensor And Helper Nlrs, Dongjiao Wang, Qibin Wu, Daowen Wang, Youxiong Que, Zheng Qing Fu

Faculty Publications

No abstract provided.

Mortality Among Transgender Persons: A Taiwan Matched-Population And Sibling-Comparison Cohort Study, Chih-Wei Hsu, Yang-Chieh Brian Chen, Liang-Jen Wang, Mu-Hong Chen, Yao-Hsu Yang, Chih-Sung Liang, Po-Yen Chen, Edward Chia-Cheng Lai

Children’s Nutrition Research Center Staff Publications

Background: Transgender persons have been reported to experience excess mortality, but evidence is dominated by Western cohorts and seldom addresses familial confounding.

Methods: We conducted a nationwide, retrospective cohort study using two prespecified comparison designs: matched population controls (1:4, matched on legal sex and birth date) and within-family cisgender sibling comparisons. Transgender persons were identified by ≥2 psychiatrist-recorded gender identity disorder diagnoses during 2001-2021; cohort entry required age ≥6 years. Participants were followed until death or December 31, 2022. We compared all-cause, external-cause (suicide and accidents), and internal-cause mortality using Cox regression adjusted for sociodemographics and medical comorbidity.

Findings: Among …

Concerns Regarding The Standard Deviation Of Individual Responses For Assessing Treatment Response Heterogeneity, Aaron R Caldwell, David B Allison, Andrew W Brown, Gary L Gadbury, Thirupathi Reddy Mokalla, R Drew Sayer, Andrew D Vigotsky

Children’s Nutrition Research Center Staff Publications

The estimation of treatment response heterogeneity (TRH) is increasingly important as medicine moves toward personalized approaches. While various statistical methods have been proposed to quantify TRH in parallel-group trials, the standard deviation of individual responses (SDIR) has gained prominence within physiological research. This method is intended to quantify individual response variation by comparing standard deviations of change scores between intervention and control groups. We acknowledge that SDIR represents an improvement over many other flawed approaches that often involve responder counting. However, SDIR has critical limitations: 1) it cannot overcome the fundamental problem of causal inference because the correlation between potential …

Microbial Signals In Primary And Metastatic Brain Tumors, Golnaz Morad, Ashish V Damania, Brenda Melendez, Bharat B Singh, Fabiana J Veguilla, Rebecca A Soto, Yasmine M Hoballah, Pranoti V Sahasrabhojane, Matthew C Wong, Mona M Ahmed, Rene N Rico, Kaitlyn N Lewis, Khalida Wani, Diana D Shamsutdinova, Rossana N Lazcano Segura, Davis R Ingram, Eric A Goethe, Abderrahman Day, Ivonne I Flores, Lauren K Mcdaniel, Manoj Chelvanambi, Sarah B Johnson, Florentia Dimitriou, Pravesh Gupta, Shivangi Oberai, M Anna Zal, Phoebe Doss, Mohamed A Jamal, Eiko Hayase, Chetna Wathoo, Lisa M Norberg, Stephanie L Jenkins, Sara Nass, Joy Gumin, Lihong Long, Jing Yang, Gina R Bradley, Mahesh Prasad Bekal, Antonio G Dono, Pavel S Pichardo-Rojas, Samuel W Andrewes, Leomar Y Ballester, Jillian S Losh, Jiyong Liang, Longfei Huo, Douglas C Nielsen, Brittany C Parker Kerrigan, Priscilla K Brastianos, Natalie Wall Fowlkes, Chia-Chi Chang, Robert R Jenq, Candelaria Gomez-Manzano, Jason T Huse, Michael A Davies, Alexander J Lazar, Krishna P Bhat, Nitin Tandon, Yoshua Esquenazi, Christine B Peterson, Vinay K Puduvalli, Frederick F Lang, Christopher D Johnston, Susan Bullman, Nadim J Ajami, Sherise D Ferguson, Jennifer A Wargo

Faculty, Staff and Student Publications

Gliomas and brain metastases are associated with poor prognosis, necessitating a deeper understanding of brain tumor biology and the development of effective therapeutic strategies. Although our group and others have demonstrated microbial presence in various tumors, recent controversies regarding cancer-type-specific intratumoral microbiota emphasize the importance of rigorous, orthogonal validation. This prospective, multi-institutional study included a total of 243 samples from 221 patients, comprising 168 glioma and brain metastases samples and 75 non-cancerous or tumor-adjacent tissues. Using stringent fluorescence in situ hybridization, immunohistochemistry and high-resolution spatial imaging, we detected intracellular bacterial 16S rRNA and lipopolysaccharides in both glioma and brain metastases …

Outcomes And Patterns Of Relapse Of Npm1 Mutated Acute Myeloid Leukemia Treated With Venetoclax Based Therapies, Wei-Ying Jen, Sanam Loghavi, Alexandre Bazinet, Alex Bataller, Ian Bouligny, Naval G Daver, Ghayas C Issa, Naszrin Arani, Emmanuel Almanza Huante, Abhishek Maiti, Guillermo Montalban-Bravo, Gautam Borthakur, Nicholas J Short, Sherry Pierce, Elias Jabbour, Guillermo Garcia-Manero, Farhad Ravandi, Hagop M Kantarjian, Courtney D Dinardo, Tapan M Kadia

Faculty, Staff and Student Publications

No abstract provided.

A Tour Of Leukemia Progress In 2025, Viewed Through The Md Anderson Leukemia Research Lens, Hagop M Kantarjian, Gautam Borthakur, Naval Daver, Courtney Dinardo, Guillermo Garcia-Manero, Ghayas Issa, Elias Jabbour, Nitin Jain, Tapan Kadia, Sanam Loghavi, Farhad Ravandi, Guilin Tang, Mary Alma Welch, William Wierda

Faculty, Staff and Student Publications

Advances in the prognostication, monitoring, and treatment of both the acute and chronic leukemias have led to drastically improved outcomes over the past 2 decades. With the advent of targeted therapies, including antibodies such as blinatumomab and inotuzumab and small molecule inhibitors, such as the BCR::ABL1 tyrosine kinase inhibitors, Bruton tyrosine kinase inhibitors, and venetoclax, the treatment landscape of leukemia has drastically changed, improving survival outcomes while relying less on overall chemotherapy intensity in many leukemia types. This progress has allowed the categorization of more leukemia types as favorable (i.e., chronic lymphocytic leukemia, younger acute lymphoblastic leukemia [patients younger than …

Brain-Wide Patterns Of Crf Receptor 1 Expression In The Transgenic Crfr1-Cre-2a-Tdtom Rat, Douglas S Engelke, Lierni Ugartemendia, Jonathan Tao, Shivakumar Rajamanikam, Fabricio H Do Monte, Nicholas J Justice

Faculty, Staff and Student Publications

The ability to manipulate neurons that express corticotropin-releasing factor (CRF) or its receptors has led to many new discoveries about how CRF signaling modulates stress-related behaviors in mice. However, similar advances in rats have been limited by the lack of reporter lines targeting CRF receptors, restricting access to behavioral paradigms that are more suitable for, or specific to, rats. Here, we provide an extensive neuroanatomical characterization of a recently generated CRFR1-Cre rat. We show that Cre and tdTomato are expressed in a pattern that closely aligns with previously reported CRFR1 expression patterns, as determined by in situ hybridization in rats …