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Fungi In Flux | Designing Regenerative Materials And Products With Mycelium, Arvind Bhallamudi

Masters Theses

As the world grapples with the escalating crisis of climate threats and environmental degradation, this research delves into the synergistic potential of design and biology, developing safe and sustainable materials for applications in prototyping, furniture and interior design. Harnessing the power of a unique organism - fungi, the study proposes an accessible, efficient, and resilient material resource system. It utilizes local waste streams and mycelium (the vegetative part of fungi) to grow functional structures. An experimental and small-scale protocol is modeled by testing bio-fabrication and bio-printing methods. The composites' performance qualities and characteristics are evaluated through mechanical testing and a …

Long-Term Mammal Herbivory On Arthropod Assemblages At Kruger National Park, South Africa, Ludzula Mukwevho, Tatenda Dalu, Frank Chidawanyika

All Peer-Reviewed Publications

Protected savannas are essential reserves for biological diversity, including endangered arthropod species, however, extreme grazing by mammals has cascading impacts on the communities and disrupts the functioning of these ecosystems globally. The current study assessed the abundance, species richness and composition of arthropods at the long-term grazing exclosures of Kruger National Park, South Africa. Pitfall traps and active searches were used to sample arthropods at the ungrazed, moderately, and heavily grazed exclosures. We found that Hymenoptera, Coleoptera, Diptera and Araneae were the most abundant orders of arthropods sampled. The abundance of multi-taxon, Diptera and Hymenoptera was significantly different between exclosures. …

Biodiversity Of Histerid Beetles (Coleoptera: Histeridae) From Brazil. I. Southern Region, Fábio Luiz De Leão Leonel, Fernando Willyan Trevisan Leivas

Insecta Mundi

This contribution adds data to the conservation and distribution of Histeridae species (Coleoptera) in the Neotropical region through a survey of Brazil’s South region. More specifically, it provides: i) a list of local Histeridae species; ii) a list of species that are inside Conservation Units in southern Brazil; iii) biotic (biome, ecoregion and associations/affinities) and abiotic information (altitude and month of occurrence) for each species. In total, 66 genera and 164 histerid beetle species were recorded, distributed in seven subfami­lies and 11 tribes. Among these, one genus and three species are new records for the region. Fifty percent of the …

Ecomorphology Of A Predatory Deep-Sea Fish Family: Does Trophic Specialization Drive Hyperspeciation?, Ryan P. Mcgonagle, David Kerstetter, Danté Fenolio, Tracey Sutton

Marine & Environmental Sciences Faculty Articles

Two of the main drivers of speciation among aquatic vertebrates are physical isolation (e.g., lakes and streams) and micro-niche availability (e.g., tropical reefs). In both regards, the mesopelagic domain of the open ocean, Earth’s second largest cumulative ecosystem (behind only the bathypelagic domain), would seem retardant. Ocean circulation makes isolation rare on both contemporary and geological time/space scales, and the lack of substrate precludes stable micro-niches. Paradoxically, some pelagic taxa demonstrate much higher-than-expected species richness on regional scales. A prime example is the dragonfish family Stomiidae, the most speciose family of mesopelagic fishes, owing largely to the subfamily Melanostomiinae (scaleless …

Community And Population Dynamics Of Diatoms In Recovering Agricultural Stream And Ubiquitous Taxon In Low-Impact Sites Across Southeastern United States, Sydney Brown

Biology Theses

Streams play vital roles to surrounding communities and provide essential ecosystem services. The protection of streams is important, legally protected under the Clean Water Act (CWA) and can be monitored through the continuous analyses of biological data, like algal or other aquatic communities. Diatoms are a group of algae characterized by rigid glass cell walls and ubiquitous distribution. They are typically identified using the morphological species concept and an ever-varying range of sizes may pose challenges in the identification process. However, when identified and applied correctly, diatoms are robust bioindicators. This research identifies and analyzes low-impact sites across the Southeastern …

Detecting Native Freshwater Mussels In Pennsylvania Waterways: Comparison & Validation Of Environmental Dna Methods, Meredith Bennett

Electronic Theses and Dissertations

North America is home to approximately one third of the world's freshwater mussel species. They are highly imperiled organisms due to habitat destruction and invasive species. Traditional surveys rely on visual identification of mussels, but individuals tend to be rare and difficult to identify. An alternative method is to extract environmental DNA (eDNA) from water samples, which has advantages over traditional sampling, including less sampling effort and fewer hazards to researchers and organisms. We conducted a review of the two main eDNA approaches: single-species detection and metabarcoding. We also developed and validated metabarcoding primers for the detection of native mussels. …

Hibernating Tricolored Bats At A Southeastern Recreation Site: Microsite Use And Public Perceptions, Rebecca Brown

All Theses

High bat mortality from white-nose syndrome (WNS) has increased the need to manage hibernation sites. Management decisions should be based on science, and when sites are accessible to the public, also need to consider human dimensions. We used Stumphouse Tunnel, a recreation site and tricolored bat (Perimyotis subflavus) hibernaculum in northwestern South Carolina, as a case study to understand potential conflicts between conservation and recreation. The population declined by 90% after the arrival of white-nose syndrome (WNS), followed by stabilization and slight increases. Because the increase was associated with more bats roosting in the publicly accessible section, a potential …

Understanding The Impacts Of Global Change: From Students To Microbes., Kylea Garces

Electronic Theses and Dissertations

Global change is a crucial process, affecting every organism, from the billions of humans on earth, to the billions of microbes in the soil belowground. Evaluating the impacts of the many drastic changes associated with global change is essential in advancing our understanding in how to mitigate these consequences. Alongside the production of basic ecological knowledge associated with these environmental changes, improving education practices to promote science literacy surrounding global change is another step towards a more optimistic future. This dissertation investigates global change from two standpoints: ecologically, where increased atmospheric nitrogen (N) deposition alters plant-fungal endophyte symbiosis, and pedagogically, …

Leveraging Population Genomics Across Two Taxonomically Diverse Non-Model Systems To Understand Patterns Of Disease Transmission And Consequences Of Hybridization, Zachary Lamar Nikolakis

Biology Dissertations - Archive

The field of population genomics has been mainly applied to questions centered around understanding molecular variation, impacts of evolutionary processes, and the history of populations. Here I leverage population genomic techniques across two different systems to understand patterns of disease transmission across heavily monitored and controlled parasite populations and consequences of hybridization between divergent rattlesnake lineages. I employed multiple genome sequencing techniques with new methods to infer close-order relatedness from highly inbred human blood fluke populations in order to understand transmission pathways across a region experiencing a resurgence in disease. I then use a comparative population genomic approach by sampling …

Assessing Recreational User Impacts, Motivations, And Knowledge On The Spread Of Invasive Plant Species In A Managed, Multiple-Use Forest Ecosystem, Crystal Strickland

All Theses

The spread of invasive plants is ecologically and economically detrimental to native ecosystems, animals, and humans. Recreation is one factor that may influence plant dispersal. The impacts of recreation on invasive plant spread are multifaceted and include the physical act of spreading and transporting seeds and vegetation to new areas on clothes, pets, and equipment, as well as the social aspect of outreach and education. Therefore, I used a multifaceted approach to assess the impact of human trafficked areas where recreational activities frequently occur on four known invasive plant species in the Clemson Experimental Forest (CEF) in South Carolina: Microstegium …

Extranodal Tumors And Chromosomal Abnormalities Associated With Non-Hodgkin Lymphoma In T(14;18)-Positive Patients, Alexis Finch

Honors Program: Senior Projects (Public)

Nebraska is mostly an agricultural state with a non-Hodgkin lymphoma (NHL) incidence rate of 20.1 per 100,000 individuals, which is greater than the US incidence rate of 19 per 100,000 individuals. It is possible that the higher incidence rate in Nebraska is due to an increase in pesticide usage. Pesticide exposure is linked to t(14;18), a genetic translocation involving the BCL2 gene that regulates apoptosis. The dysregulation of apoptosis could contribute to the proliferation of NHL. This exploratory study utilized the Nebraska Lymphoma Study Group, which consists of patients with non-Hodgkin lymphoma, Hodgkin disease, or some other neoplastic hematologic disorder …

Heat Stress Effects On Culex Pipiens Mosquitoes, Valeria Montoya

Theses, Dissertations and Culminating Projects

Extreme weather events such as heat waves are becoming more frequent. This is a concern for both humans and other organisms like insects. Culex pipiens f. molestus mosquitoes are primary vectors of diseases including equine encephalitis, West Nile virus, Rift valley fever, and St. Louis encephalitis. With Cx. pipiens f. molestus being the primary vector of West Nile virus in North America, it is important to understand how more frequent heat waves affect their population dynamics. In this study, I compared the thermal tolerance of larval Cx. pipiens f. molestus that were exposed to prior heat stress to those that …

Toward Accelerometer Recording Of Pit Viper Foraging Behavior In Nature: Validation And Case Study With Timber Rattlesnakes (Crotalus Horridus), Morgan L. Thompson

Biology Theses

Accelerometer dataloggers are becoming increasingly common in field studies of animal behavior. Among the most difficult study subjects, and consequently, the most ideal candidates for accelerometer applications, are those for which simultaneous observation of key fitness-determining behaviors, such as foraging, across a sample of individuals in the wild is traditionally impossible or restricted to the use of proxy measures. This is the case for many solitary vertebrate predators, such as all pit vipers (Viperidae; Crotalinae). Large-bodied pit vipers are ambush (sit-and-wait) specialists that represent uniquely challenging and intriguing study subjects in predator ecology. Unlike many comparable avian or mammalian terrestrial …

Characterizing Spatial Variability In Soil Co2 Fluxes In The Chihuahuan Desert Using Geostatistical Techniques, Viridiana Orona

Open Access Theses & Dissertations

Spatial variability in soil CO2 efflux across landscapes is an important feature of the â??Critical Zoneâ?? within dryland ecosystems. In dryland critical zones, resources are often distributed in patches or resource islands. Although this is particularly true in natural settings, the significance of spatial variability in CO2 efflux and its patterns also extends to dryland agriculture. In both irrigated and unirrigated systems, human management practices can significantly impact both organic and inorganic carbon cycling processes, highlighting the importance of studying CO2 efflux in these systems. We examined the spatial patterns of soil CO2 efflux and quantified the magnitude and scale …

Fractally Sampling Diversity-Environment Relationships To Understand Plant Assemblage Health Across Spatial Scales, Elizabeth G. Simpson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Humans influence the health of ecosystems and rely on healthy ecosystems to support their livelihoods and well-being. By looking at how the parts of ecosystems interact we can understand and improve ecosystem health. Ecosystem interactions change across spatial scales or different size patches of area. For example, individual organisms interact with each other at small spatial scales, while at large spatial scales, communities of organisms interact with weather conditions. However, many research studies do not look at how ecosystem interactions change across spatial scales. To address this gap in ecological research, I use a fractal sampling design which samples at …

The Effects Of Recent Climate Change On Spring Phenology, With A Special Focus On Patterns Of Bee Foraging, Michael Stemkovski

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The date on which plants flower and on which bees begin to pollinate varies year-to-year depending on differences in weather. This seasonal timing is known as phenology, and it is already clear that climate change has pushed the spring phenology of many species earlier by increasing temperatures. This is particularly clear in flowering plants, but studying how and why the phenology of pollinators is shifting is more difficult. Most flowering plants rely on pollinators such as bees for their reproduction, and most bees rely on flowers for their sustenance, so bee and flower phenology has to overlap for the crucial …

Habitat Selection By Desert Carnivores: How Water, Intraguild Predation, And Prey Impact Animal Space Use, Nadine A. Pershyn

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Across North America large carnivores have lost nearly half of their historic range, creating openings for smaller (< 15 kg) carnivores to take over their ecological roles. Coyotes (Canis latrans) have seen a significant range expansion, including into arid deserts. It was believed that the addition of artificial water sources by humans allowed coyotes to expand into desert ecosystems where they were previously unable to survive. The kit fox (Vulpes macrotis) is a small, desert-adapted carnivore with declining populations that faces threats from coyotes of intraguild predation. This is when carnivores compete over the same prey, and the larger carnivore (intraguild predator) kills the smaller carnivore (intraguild …

Factors That Affect Home Range Of Timber Rattlesnakes (Crotalus Horridus) In Northwest Arkansas, Bannon Gallaher

Graduate Theses and Dissertations

Conservation of animal populations requires knowledge of their habitat and spatial needs. Quantifying spatial requirements involves the analysis of home range. We examined the effects of sex, body size (SVL), body condition (log mass/log SVL), and year on home range in Timber Rattlesnakes (Crotalus horridus) in Northwest Arkansas. Individual locality data from an ongoing, 22+ year radio-telemetry study in Madison Co., Arkansas were analyzed using both minimum convex polygon (MCP) and Kernel Density Estimates (KDE). Plots of the number of sequential observations versus home range (MCP and KDE) determined that a minimum of 25 locations per individual per active season …

Bryophytes Of Goochland County, Virginia, Mikayla Quinn

Honors Theses

Bryophytes are non-vascular land plants that include mosses, liverworts, and hornworts. Although easier to overlook because of their smaller size, bryophytes are a fundamental part of the ecosystem. As such, maintaining record of their biodiversity is important. Yet, records of bryophyte species in Goochland County, VA were low compared to more thoroughly documented counties such as Prince Edward County. This study expands the documentation of bryophyte flora and presents a checklist of bryophyte species found Virginia’s Goochland County from 2020-2023. Fieldwork conducted at public and privately-owned properties throughout the county between January 2020 and March 2023 yielded 702 specimens that …

An Ecological Assessment Of The Deep-Pelagic Fish Genus Cyclothone (Gonostomatidae; Stomiiformes), Possibly The World’S Most Abundant Fishes, In The Gulf Of Mexico, Olivia C. North-Menthonnex

All HCAS Student Capstones, Theses, and Dissertations

The fish genus Cyclothone is considered the most abundant vertebrate taxon on Earth. Despite this assertion, very few detailed ecological studies of this genus exist for any site in the World Ocean, largely due to the lack of expertise (and willingness) to identify specimens from existing sample sets. This study will provide a species-level description of the abundance, vertical distribution, and size structuring of the genus Cyclothone in the Gulf of Mexico, a hyper-diverse, deep-pelagic ecosystem that is increasingly impacted by anthropogenic disturbances. As the putative most-abundant fishes in the ecosystem, this characterization is critically needed for a holistic understanding …

A Tool For Selecting Ecological Survey Locations In The Southeastern United States Using A Matched-Paired Design, David R. Nelsen, Aaron G. Corbit, Harvey Alférez

Campus Research Month

Site selection is a critical step for conducting an ecological survey. Inappropriate site selection may lead to confounding variables being included in your data and result in incorrect or inconclusive conclusions ^1,2. The Jorō spider, Trichonephila clavata, is an invasive species ³ that is currently expanding through the Southeaster United States⁴. However, it remains unclear how far the spider will spread and what impact the spider will have on local ecosystems. Early results do suggest that Jorōs are having a negative impact on native orb-weaving spiders⁴, but more systematic research is needed to verify …

Modeling Collective Cell Behavior In Cancer: Perspectives From An Interdisciplinary Conversation, Frederick R Adler, Alexander R A Anderson, Abhinav Bhushan, Paul Bogdan, Jose Javier Bravo-Cordero, Amy Brock, Yun Chen, Edna Cukierman, Kathleen E Delgiorno, Gerald V Denis, Meghan C Ferrall-Fairbanks, Zev Jordan Gartner, Ronald N Germain, Deborah M Gordon, Ginger Hunter, Mohit Kumar Jolly, Loukia Georgiou Karacosta, Karthikeyan Mythreye, Parag Katira, Rajan P Kulkarni, Matthew L Kutys, Arthur D Lander, Ashley M Laughney, Herbert Levine, Emil Lou, Pedro R Lowenstein, Kristyn S Masters, Dana Pe'er, Shelly R Peyton, Manu O Platt, Jeremy E Purvis, Gerald Quon, Jennifer K Richer, Nicole C Riddle, Analiz Rodriguez, Joshua C Snyder, Gregory Lee Szeto, Claire J Tomlin, Itai Yanai, Ioannis K Zervantonakis, Hannah Dueck

Faculty, Staff and Student Publications

Collective cell behavior contributes to all stages of cancer progression. Understanding how collective behavior emerges through cell-cell interactions and decision-making will advance our understanding of cancer biology and provide new therapeutic approaches. Here, we summarize an interdisciplinary discussion on multicellular behavior in cancer, draw lessons from other scientific disciplines, and identify future directions.

Seasonal Dynamics And Glycerol-3-Phosphate Dehydrogenase Variability In Ixodes Scapularis Tick Population In Eastern Texas, Daniela Soto

Graduate Research Conference

Objectives: 1. Document seasonal population dynamics of I. scapularis in eastern Texas. 2. Explore variability of GPDH in I. scapularis ticks in eastern Texas and relate data to its population dynamics.

Detection Of A Diverse Endophyte Assemblage Within Fungal Communities Associated With The Arundo Leaf Miner, Lasioptera Donacis (Diptera: Cecidomyiidae), Marie-Claude Bon, John A. Goolsby, Guy Mercadier, Fatiha Guermache, Javid Kashef, Massimo Cristofaro, Ann T. Vacek, Alan Kirk

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

The larvae of Lasioptera donacis Coutin feed on fungal communities lining galleries within the mesophyll of leaf sheaths of Arundo donax in an aggregative manner. It has been stated that L. donacis could have established a fundamental symbiotic relationship with one fungus, although the fungal composition of these communities remains unsettled. Using a culture-dependent approach and ITS sequencing, the present work characterizes and compares the fungal communities associated with L. donacis in Eurasia with the endophytes of A. donax in Texas where L. donacis is absent. The 65 cultivable isolates obtained from L. donacis fungal communities were sorted into 15 …

Detection Of A Diverse Endophyte Assemblage Within Fungal Communities Associated With The Arundo Leaf Miner, Lasioptera Donacis (Diptera: Cecidomyiidae), Marie-Claude Bon, John A. Goolsby, Guy Mercadier, Fatiha Guermache, Javid Kashef, Massimo Cristofaro, Ann T. Vacek, Alan Kirk

School of Earth, Environmental, & Marine Sciences Faculty Publications

The larvae of Lasioptera donacis Coutin feed on fungal communities lining galleries within the mesophyll of leaf sheaths of Arundo donax in an aggregative manner. It has been stated that L. donacis could have established a fundamental symbiotic relationship with one fungus, although the fungal composition of these communities remains unsettled. Using a culture-dependent approach and ITS sequencing, the present work characterizes and compares the fungal communities associated with L. donacis in Eurasia with the endophytes of A. donax in Texas where L. donacis is absent. The 65 cultivable isolates obtained from L. donacis fungal communities were sorted into 15 …

Assessment Of Queensnakes And Northern Watersnakes Living In A Trashy Creek, Noah Kurtz

Liberty University Research Week

Undergraduate

Basic

Floral Visitors Of A Colorado Endemic Chasmophyte, Telesonix Jamesii (Saxifragaceae), Andrew G. Gaier, Erin Manzitto-Tripp, Julian Resasco

Western North American Naturalist

Telesonix jamesii, a rare and imperiled species of perennial saxifrage, is restricted to rocky habitats at high elevations across 21 isolated, known populations in the southern Rocky Mountains of Colorado and New Mexico. Despite its imperiled conservation status, very little is known about the natural history of T. jamesii. We studied pollination of this species during the summers of 2019–2021 at multiple locations on Pikes Peak, Colorado. We conducted a total of 899 min of pollinator surveys, identifying all floral visitors during this time period. We then examined floral visitors for the presence of T. jamesii pollen to …

Ecological Impacts Of Invasive Crayfish In A Naïve Riparian System, Gregor L. Hamilton

Biology ETDs

Aquatic and riparian food webs are inextricably linked. In deserts, they provide critical energy subsidies to surrounding terrestrial food webs, but these vital subsidies are not guaranteed; streams in arid lands are especially sensitive to perturbations. In this dissertation, I investigated the role of an oft-cited threat to stream communities in the lower Colorado River basin: non-native crayfish. I contributed to methodologies in passive integrated transponder use and length-weight relationships in crayfish to aid future research. I leveraged stable isotope analysis of δ¹⁵N, δ¹³C, and δ²H to understand crayfish role in the food webs. …

Physiological And Transcriptomic Responses Of Two Artemisia Californica Populations To Drought: Implications For Restoring Drought-Resilient Native Communities, Hagop S. Atamian Dr., Jennifer L. Funk

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

As climate change brings drier and more variable rainfall patterns to many arid and semi-arid regions, land managers must re-assemble appropriate plant communities for these conditions. Transcriptome sequencing can elucidate the molecular mechanisms underlying plant responses to changing environmental conditions, potentially enhancing our ability to screen suitable genotypes and species for restoration. We examined physiological and morphological traits and transcriptome sequences of coastal and inland populations of California sagebrush (Artemisia californica), a critical shrub used to restore coastal sage scrub vegetation communities, grown under low and high rainfall environments. The populations are located approximately 36 km apart but …

Scale-Dependent Spatial Ecology Of Paleotropical Leaf Litter Ants (Hymenoptera: Formicidae), Lillian Germeroth, Theodore Sumnicht, Robin M. Verble

Biological Sciences Faculty Research & Creative Works

The Grain for Which an Observer Conducts a Study is an Important Determinant of its Outcome. Studies of Ants Have Considered Spatial Grains Spanning from Single Meters to Entire Forest Ecosystems and Found Patterns Related to Nutrient Availability, Leaf Litter Depth, Disturbance, and Forest Composition. Here, We Examine a Bornean Leaf Litter Ant Community at Small (1–4 M) and Large (50–250 M) Spatial Scales and Consider the Differences in Community Structure using Structured 1 M2 Quadrats Sampled Via Leaf Litter Sifting and Berlese Extraction. We Found that Small-Scale Patterns in Ant Abundance and Richness Did Not Spatially Auto correlate within …