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Program And Abstracts Of The 56th Annual Conference Of The Mississippi Entomological Association, October 27–28, 2009, Mississippi Entomological Association Feb 2010

Program And Abstracts Of The 56th Annual Conference Of The Mississippi Entomological Association, October 27–28, 2009, Mississippi Entomological Association

Midsouth Entomologist

No abstract provided.


Our Places To Tell Stories: Preliminary Program Springs Preserve, Public Lands Institute Feb 2010

Our Places To Tell Stories: Preliminary Program Springs Preserve, Public Lands Institute

Our Places to Tell Stories

Our Places Tell Stories is an innovative conference for educators and others working at public lands, schools, nature centers, museums, zoos, aquaria, and all nature and heritage rich places.

This list of presentations represents a preliminary program of sessions. A final program will be emailed to all registrants prior to the conference. Thank you for your interest, and we look forward to seeing you at the Springs!


Ducks, Geese, And Swans Of The World: Sources Cited, Paul A. Johnsgard Jan 2010

Ducks, Geese, And Swans Of The World: Sources Cited, Paul A. Johnsgard

Ducks, Geese, and Swans of the World by Paul A. Johnsgard

350+ citations


What Is The Taxonomic Identity Of Minnesota Wolves?, L. David Mech Jan 2010

What Is The Taxonomic Identity Of Minnesota Wolves?, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

The taxonomic identity of the historical and current wolf (Canis lupus L., 1758 or Canis lycaon Schreber, 1775 or their hybrids) population in Minnesota (MN) and the Great Lakes region has been, and continues to be, controversial. So too does its legal status under the U.S. Endangered Species Act. This review summarizes the morphological and genetic information about that population and concludes that historically the MN population consisted of a gray wolf (C. lupus) in the west and an eastern type (Canis lupus lycaon or C. lycaon) in the east with intergrades or hybrids between …


Liana Host Preference And Implications For Deciduous Forest Regeneration, Laura M. Ladwig, Scott J. Meiners Jan 2010

Liana Host Preference And Implications For Deciduous Forest Regeneration, Laura M. Ladwig, Scott J. Meiners

Faculty Research & Creative Activity

Lianas have the potential to shape forest communities and alter forest regeneration. However, impacts of lianas on forest regeneration, particularly in temperate forests, are largely unstudied. To understand potential liana impacts on the community we need to first know the location and intensity of liana burdens on host trees. We examined liana-tree host references within a series of young regenerating deciduous forests in the Piedmont region of New Jersey, USA. Established trees ($ 5 cm dbh) and the lianas associated with each tree were surveyed in 2008. The five most abundant liana species were Celastrus orbiculatus, Lonicera japonica, Parthenocissus quinquefolia, …


Post-­Fire Alterations In Mechanical Strength Of Leaves In Heteromeles Arbutifolia, Andrew Hair, Madeline Dilascia, Nick Novella, Taylor Wurdeman Jan 2010

Post-­Fire Alterations In Mechanical Strength Of Leaves In Heteromeles Arbutifolia, Andrew Hair, Madeline Dilascia, Nick Novella, Taylor Wurdeman

Featured Research

Wildfires in the Santa Monica mountains in Southern California burn down a decent percentage of the local vegetation. However, some plants like Heteromeles Arbutifolia, are considered to be resprouters because they return a mere two years after being burnt down. It is hypothesized that the artificially browsed resprout leaves will have a lower tensile strength than both the young and adult leaves because they use a lower amount of carbohydrates per unit-leaf-area. Data showed that although there was a slight different between the young leaves and the adult leaves of the Heteromeles, there was no statistically significant difference between the …


Waterfowl Of North America: Sources, Paul A. Johnsgard Jan 2010

Waterfowl Of North America: Sources, Paul A. Johnsgard

Waterfowl of North America, Revised Edition (2010) by Paul A. Johnsgard

Approximately 500 citations.

See also the updated bibliography in the 2010 supplement: "North America’s Ducks, Geese and Swans in the 21st Century"


Linking Environmental Nutrient Enrichment And Disease Emergence In Humans And Wildlife, Pieter T. J. Johnson, Alan R. Townsend, Cory C. Cleveland, Patricia M. Glibert, Robert W. Howarth, Valerie J. Mckenzie, Eliska Rejmankova, Mary H. Ward Jan 2010

Linking Environmental Nutrient Enrichment And Disease Emergence In Humans And Wildlife, Pieter T. J. Johnson, Alan R. Townsend, Cory C. Cleveland, Patricia M. Glibert, Robert W. Howarth, Valerie J. Mckenzie, Eliska Rejmankova, Mary H. Ward

Ecosystem and Conservation Sciences Faculty Publications

Worldwide increases in human and wildlife diseases have challenged ecologists to understand how large-scale environmental changes affect host–parasite interactions. One of the most profound changes to Earth's ecosystems is the alteration of global nutrient cycles, including those of phosphorus (P) and especially nitrogen (N). Along with the obvious direct benefits of nutrient application for food production, anthropogenic inputs of N and P can indirectly affect the abundance of infectious and noninfectious pathogens. The mechanisms underpinning observed correlations, however, and how such patterns vary with disease type, have long remained conjectural. Here, we highlight recent experimental advances to critically evaluate the …


Are Forested Buffers An Effective Conservation Strategy For Riparian Fauna? An Assessment Using Meta-Analysis, Laurie B. Marczak, Takashi Sakamaki, Shannon L. Turvey, Isabelle Deguise, Sylvia L. R. Wood, John S. Richardson Jan 2010

Are Forested Buffers An Effective Conservation Strategy For Riparian Fauna? An Assessment Using Meta-Analysis, Laurie B. Marczak, Takashi Sakamaki, Shannon L. Turvey, Isabelle Deguise, Sylvia L. R. Wood, John S. Richardson

Biological Sciences Faculty Publications

Historically, forested riparian buffers have been created to provide protection for aquatic organisms and aquatic ecosystem functions. Increasingly, new and existing riparian buffers are being used also to meet terrestrial conservation requirements. To test the effectiveness of riparian buffers for conserving terrestrial fauna, we conducted a meta-analysis using published data from 397 comparisons of species abundance in riparian buffers and unharvested (reference) riparian sites. The response of terrestrial species to riparian buffers was not consistent between taxonomic groups; bird and arthropod abundances were significantly greater in buffers relative to unharvested areas, whereas amphibian abundance decreased. Edge-preferring species were more abundant …


Latitudinal Variation In Seasonal Activity And Mortality In Ratsnakes (Elaphe Obsoleta), Jinelle H. Sperry, Gabriel Blouin-Demers, Gerardo L.F. Carfagno, Patrick J. Weatherhead Jan 2010

Latitudinal Variation In Seasonal Activity And Mortality In Ratsnakes (Elaphe Obsoleta), Jinelle H. Sperry, Gabriel Blouin-Demers, Gerardo L.F. Carfagno, Patrick J. Weatherhead

Biology Faculty Publications

The ecology of ectotherms should be particularly affected by latitude because so much of their biology is temperature dependent. Current latitudinal patterns should also be informative about how ectotherms will have to modify their behavior in response to climate change. We used data from a total of 175 adult black ratsnakes (Elaphe obsoleta) radio tracked in Ontario, Illinois, and Texas, a latitudinal distance of > 1500 km, to test predictions about how seasonal patterns of activity and mortality should vary with latitude. Despite pronounced differences in temperatures among study locations, and despite ratsnakes in Texas not hibernating and switching from diurnal …


Fall 2010, Nsu Oceanographic Center Jan 2010

Fall 2010, Nsu Oceanographic Center

Currents

No abstract provided.


Variation In Leaf Stomatal Traits Of 28 Tree Species In Relation To Gas Exchange Along An Edaphic Gradient In A Bornean Rain Forest, Sabrina E. Russo, Whitney Logan Cannon, Christian Elowsky, Sylvester Tan, Stuart J. Davies Jan 2010

Variation In Leaf Stomatal Traits Of 28 Tree Species In Relation To Gas Exchange Along An Edaphic Gradient In A Bornean Rain Forest, Sabrina E. Russo, Whitney Logan Cannon, Christian Elowsky, Sylvester Tan, Stuart J. Davies

School of Biological Sciences: Faculty Publications

Premise of the study: Quantifying variation in functional traits associated with shifts in the species composition of plant communities along resource gradients helps identify environmental attributes important for community assembly. Stomates regulate the balance between carbon assimilation and water status in plants. If environmental attributes affecting photosynthetic water-use efficiency govern species distribution along an edaphic gradient, then adaptive variation in stomatal traits of plant species specializing on different soils should reflect belowground resource availability.

Methods: We tested this hypothesis by quantifying stomatal trait variation in understory saplings of 28 Bornean tree species in relation to gas exchange and …


Comparative Morphology Of Two Sympatric Species Of Hedgehog In Ikh Nart Nature Reserve, Mongolia, Richard P. Reading, David Kenny, Sodnomphil Batdorj, James Murdoch Jan 2010

Comparative Morphology Of Two Sympatric Species Of Hedgehog In Ikh Nart Nature Reserve, Mongolia, Richard P. Reading, David Kenny, Sodnomphil Batdorj, James Murdoch

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

Daurian hedgehog (Mesechinus dauuricus) and long-eared hedgehog (Hemiechinus auritus) inhabit Ikh Nart Nature Reserve, Mongolia and both species appear to occupy similar niches. We gathered morphological measurements to test for differences between species and sexes. We hoped to gain insight into the mechanisms that allow the species to coexist. We collected morphometric data from 10 long-eared hedgehogs (6 male, 4 female) and 18 Daurian hedgehogs (10 male, 7 female, and 1 unknown sex). Only total body length in Daurian hedgehogs differed significantly between males and females. Otherwise, we found no significant differences between morphometric measures of …


Natural History Of Belonuchus Nordmann Spp. And Allies (Coleoptera: Staphylinidae) In Heliconia L. (Zingiberales: Heliconiaceae) Flower Bracts, J. H. Frank, R. Barrera Jan 2010

Natural History Of Belonuchus Nordmann Spp. And Allies (Coleoptera: Staphylinidae) In Heliconia L. (Zingiberales: Heliconiaceae) Flower Bracts, J. H. Frank, R. Barrera

Insecta Mundi

Adults, and in some species larvae, of several members of Belonuchus Nordmann (Coleoptera: Staphylinidae: Staphylininae) and a few related genera seem to be to various degrees consistently associated with flower bracts of the genus Heliconia (Zingiberales: Heliconiaceae). They are predators and eat various dipterous and lepidopterous larvae in that habitat. Adults of at least Belonuchus cephalotes (Sharp) and Odontolinus fasciatus Sharp are able to immerse completely in water to capture larvae and/or pupae of mosquitoes (Culicidae).

Adults and larvae of Belonuchus satyrus Erichson, and adults of B. cacao Blackwelder and B. rufipennis (F.) were found in water-filled flower bracts of …


Diversity Of Interactions: A Metric For Studies Of Biodiversity, Lee A. Dyer, Thomas R. Walla, Harold F. Greeney, John O. Stireman Iii, Rebecca F. Hazen Jan 2010

Diversity Of Interactions: A Metric For Studies Of Biodiversity, Lee A. Dyer, Thomas R. Walla, Harold F. Greeney, John O. Stireman Iii, Rebecca F. Hazen

Biological Sciences Faculty Publications

Multitrophic interactions play key roles in the origin and maintenance of species diversity, and the study of these interactions has contributed to important theoretical advances in ecology and evolutionary biology. Nevertheless, most biodiversity inventories focus on static species lists, and prominent theories of diversity still ignore trophic interactions. The lack of a simple interaction metric that is analogous to species richness is one reason why diversity of interactions is not examined as a response or predictor variable in diversity studies. Using plant-herbivore-enemy trophic chains as an example, we develop a simple metric of diversity in which richness, diversity indices (e.g., …


Temperature Dependent Development Of The Egg-Larval Parasitoid Chelonus Oculator On The Factitious Host, Ephestia Cautella, Hi̇lal Tunca, Cem Özkan, Ahmet Neşet Kilinçer Jan 2010

Temperature Dependent Development Of The Egg-Larval Parasitoid Chelonus Oculator On The Factitious Host, Ephestia Cautella, Hi̇lal Tunca, Cem Özkan, Ahmet Neşet Kilinçer

Turkish Journal of Agriculture and Forestry

Development, fecundity, and longevity of the egg-larval parasitoid Chelonus oculator Panzer (Hymenoptera: Braconidae) were studied on the factitious host, Ephestia cautella (Walker) (Lepidoptera: Pyralidae) at 4 different temperatures (15 °C, 20 °C, 25 °C, and 30 °C). Developmental time from egg to adult was inversely related to temperature. Egg to adult development time ranged from 188 days at 15 °C to 28 days at 30 °C. Complete development required 489 degree-days with a developmental threshold of 12.5 °C. Adult male and female longevity ranged from 43 days at 20 °C to 17 days at 30 °C. Maximum production of offspring …


Trophic Implications Of Light Reductions For Amphibolis Griffithii Seagrass Fauna, Adam Gartner Jan 2010

Trophic Implications Of Light Reductions For Amphibolis Griffithii Seagrass Fauna, Adam Gartner

Theses: Doctorates and Masters

The ongoing threat of seagrass loss from reduced light availability, coupled with our lack of knowledge of associated trophic responses has motivated this characterization of the flow-on effects of light reductions to Amphibolis griffithii seagrass fauna. Recently, field manipulations of varying light reductions, induced disturbances in a A. griffithii seagrass meadow that have been shown to effect potential food resources and the structural complexity of seagrass habitats for macroinvertebrates. This offered the opportunity to assess the flow-on effects to seagrass for fauna, a topic that has seldom been examined. This study investigated the effects of different light reduction intensity (high: …


Multiple Endpoints Of Endocrine Disruption In Gravid Watersnakes (Colubridae:Nerodia) As A Function Of Ingestion Of A Common Herbicide, Lorin Anne Neuman-Lee Jan 2010

Multiple Endpoints Of Endocrine Disruption In Gravid Watersnakes (Colubridae:Nerodia) As A Function Of Ingestion Of A Common Herbicide, Lorin Anne Neuman-Lee

Masters Theses

Ecotoxicological studies that focus on a single endpoint might not accurately represent the true ecological effects of a contaminant. Exposure to atrazine, a widelyused herbicide, disrupts endocrine function and sexual development in amphibians, but studies involving reptilian species are lacking. This study examines several effects of atrazine ingestion on gravid females and neonates exposed in utero of the Northern Watersnake (Nerodia sipedon). Twenty-five gravid N. sipedon were collected and kept in the lab for the entirety of their gestation period. Each snake received one of four doses of atrazine: control, 2, 20, or 200 ppb. Blood samples were drawn each …


Combining A Dispersal Model With Network Theory To Assess Habitat Connectivity, Todd R. Lookingbill, Robert H. Gardner, Joseph R. Ferrari, Cherry E. Keller Jan 2010

Combining A Dispersal Model With Network Theory To Assess Habitat Connectivity, Todd R. Lookingbill, Robert H. Gardner, Joseph R. Ferrari, Cherry E. Keller

Geography and the Environment Faculty Publications

Assessing the potential for threatened species to persist and spread within fragmented landscapes requires the identification of core areas that can sustain resident populations and dispersal corridors that can link these core areas with isolated patches of remnant habitat. We developed a set of GIS tools, simulation methods, and network analysis procedures to assess potential landscape connectivity for the Delmarva fox squirrel (DFS; Sciurus niger cinereus), an endangered species inhabiting forested areas on the Delmarva Peninsula, USA. Information on the DFS’s life history and dispersal characteristics, together with data on the composition and configuration of land cover on the peninsula, …


Phylogenetic Relationships Of Some Turkish Crocus (Iridaceae) Taxa Based On Morphological And Anatomical Characters, Fati̇h Coşkun, Selami̇ Selvi̇, Fati̇h Satil Jan 2010

Phylogenetic Relationships Of Some Turkish Crocus (Iridaceae) Taxa Based On Morphological And Anatomical Characters, Fati̇h Coşkun, Selami̇ Selvi̇, Fati̇h Satil

Turkish Journal of Botany

In this study, relationships among the 15 taxa of the genus Crocus L. distributed in Turkey were analysed using 29 morphological and 4 anatomical characters. Analysis of the data set utilising maximum parsimony criterion with Branch-and-Bound search algorithm yielded 32 most parsimonious trees. Bootstrap analysis with the majority rule consensus algorithm generated a consensus tree supporting some branches. Our data mostly did not agree with the previous sectional and serial treatments. The most interesting result was the condition of Crocus pallasii, which was previously included in the section Crocus. C. pallasii showed a sister group relationship with C. cancellatus from …


Morphological Analyses Of Some Palaearctic Stenopogon Loew, 1847 (Diptera: Asilidae: Stenopogoninae) Based On The Spermatheca Structure, Abdullah Hasbenli̇, Fatma Bayrakdar, Selami̇ Candan, Üzeyi̇r Çağlar Jan 2010

Morphological Analyses Of Some Palaearctic Stenopogon Loew, 1847 (Diptera: Asilidae: Stenopogoninae) Based On The Spermatheca Structure, Abdullah Hasbenli̇, Fatma Bayrakdar, Selami̇ Candan, Üzeyi̇r Çağlar

Turkish Journal of Zoology

Females of some Stenopogon species were compared both systematically and phylogenetically utilizing spermatheca morphology. The spermathecae of 8 species and 2 subspecies of Stenopogon (S. coracinus, S. elongatus S. flavibarbis, S. junceus, S. laevigatus, S. nigriventris, S. schisticolor, S. strateagus, S. sabaudus harpax, and S. xanthotrichus xanthomelas) were examined using a scanning electron microscope and subsequently analyzed with cluster analysis. Taxa of evaluated Stenopogon were found to be separated into 2 primary groups, A and B, based on the number of spirals and the diameter of the reservoir. Group A further separated into the sub-groups A1, A2, and A3, while …


Behavior And Habitat Use Of Greater Sandhill Cranes Wintering In East Tennessee, David A. Aborn Jan 2010

Behavior And Habitat Use Of Greater Sandhill Cranes Wintering In East Tennessee, David A. Aborn

Proceedings of the North American Crane Workshop

An increase of eastern greater sandhill cranes (Grus canadensis tabida) stopping over and wintering at the Hiwassee Wildlife Refuge in east Tennessee has led to overcrowding at the refuge, resulting in crane use of private property. Proper management is needed to prevent the cranes from becoming a nuisance, increase the suitability of the refuge for waterfowl, and reduce the likelihood of a disease outbreak. Observations of cranes revealed that cranes spent 83% of their time foraging, alert, or preening. Sandhill cranes foraged primarily in agricultural and grassy fields, whereas mudflats and shallow water were used for preening and …


Population Status And Geographic Distribution Of Greater Sandhill Cranes In The Mid-Continent Population, Gary L. Krapu, David A. Brandt Jan 2010

Population Status And Geographic Distribution Of Greater Sandhill Cranes In The Mid-Continent Population, Gary L. Krapu, David A. Brandt

Proceedings of the North American Crane Workshop

Number and geographic distribution of greater sandhill cranes (Grus canadensis tabida) in the mid-continent population (MCP) of sandhill cranes were evaluated. G. c. tabida comprised 7 of 133 (5.3%) individuals of 3 subspecies which projects to 31,579 ± 11,661 (SE) individuals in an estimated spring MCP of 600,000 cranes. From a platform transmitting terminal (PTT)-marked sample representative of the geographic distribution of G. c. tabida, 10 of 13 (77%) settled during the breeding season in east-central Canada/Minnesota, including 4 in northwestern Minnesota, 4 in Manitoba (2 at sites near the Minnesota border), and 2 in Ontario. Three …


Countability Of Sandhill Cranes In Aerial Surveys, Douglas H. Johnson, John W. Solberg, Courtney L. Amundson Jan 2010

Countability Of Sandhill Cranes In Aerial Surveys, Douglas H. Johnson, John W. Solberg, Courtney L. Amundson

Proceedings of the North American Crane Workshop

Aerial surveys are used to monitor populations of many wildlife species, including sandhill cranes (Grus canadensis). In addition to the usual problems of detectability (involving both availability and perceptibility), aerial surveys of concentrated animals are subject to countability issues; from a rapidly moving aircraft, observers cannot count or accurately estimate the number of animals in a large group. Calibration is sometimes performed in an effort to adjust aerial counts for incomplete detectability and countability by calculating the ratio of animals actually in a group to the number in the group estimated from the aircraft. Here we explore alternative, …


Carbon Dynamics In A Phragmites Australis Invaded Riparian Wetland, Steven Walters Jan 2010

Carbon Dynamics In A Phragmites Australis Invaded Riparian Wetland, Steven Walters

School of Natural Resources: Dissertations, Theses, and Student Research

Invasive plant species are widely recognized as a major threat to biodiversity and ecosystem stability. Wetland ecosystems tend to be much more susceptible to invasions because of their location on the landscape where water, nutrients, and the impacts of disturbances accumulate. Invasive plants have the ability to alter ecosystem processes and community/population dynamics. The ability of invasive plants to alter these processes can have profound economic consequences. In the United States, control of invasive wetland species alone costs approximately 155 million dollars annually. The state of Nebraska spends 2 million dollars annually controlling invasive plant species in the Platte River …


Yield Potential And Resource-Use Efficiency Of Maize Systems In The Western U. S. Corn Belt, Patricio Grassini Jan 2010

Yield Potential And Resource-Use Efficiency Of Maize Systems In The Western U. S. Corn Belt, Patricio Grassini

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Maize demand for food, livestock feed, and biofuel is expected to increase substantially. The Western U.S. Corn Belt accounts for 23% of U.S. maize production, and irrigated maize accounts for 43 and 58% of maize land area and total production, respectively, in this region. The most sensitive parameters (yield potential [YP], water-limited yield potential [YP-W], yield gap between actual yield and YP, and resource-use efficiency) governing performance of maize systems in the region are lacking. A simulation model was used to quantify YP under irrigated and rainfed conditions based on weather data, soil properties, and crop management at 18 locations. …


Characterizing The Vertical Structure And Structural Diversity Of Florida Oak Scrub Vegetation Using Discrete-Return Lidar, James J. Angelo Jan 2010

Characterizing The Vertical Structure And Structural Diversity Of Florida Oak Scrub Vegetation Using Discrete-Return Lidar, James J. Angelo

Electronic Theses and Dissertations

Vertical structure, the top-to-bottom arrangement of aboveground vegetation, is an important component of forest and shrubland ecosystems. For many decades, ecologists have used foliage height profiles and other measures of vertical structure to identify discrete stages in post-disturbance succession and to quantify the heterogeneity of vegetation. Such studies have, however, required resource-intensive field surveys and have been limited to relatively small spatial extents (e.g.,


Canopy Demographics At The Firestone Reserve, Costa Rica, Gizelle M. Pera Jan 2010

Canopy Demographics At The Firestone Reserve, Costa Rica, Gizelle M. Pera

CMC Senior Theses

Though Costa Rica has suffered numerous bouts of deforestation to its valuable tropical rainforest area, especially in the 1970s, it has become a leader in its efforts to regenerate and restore its rainforest. However, studies and protocols for the assessment of forest regeneration are urgently required. Research has shown that the percentage of light penetrating the canopy floor, or light fraction, is a good indicator of rainforest maturity. In this study, digital rectilinear photography and a global positioning system receiver were used to survey the Firestone Reserve inCosta Rica in order to measure the light fraction differences between primary/riparian …


Effects Of Moist Chilling, Gibberellic Acid, And Scarification On Seed Dormancy In The Rare Endemic Pedicularis Olympica (Scrophulariaceae), Serap Kirmizi, Gürcan Güleryüz, Hülya Arslan, Fatma Selcen Sakar Jan 2010

Effects Of Moist Chilling, Gibberellic Acid, And Scarification On Seed Dormancy In The Rare Endemic Pedicularis Olympica (Scrophulariaceae), Serap Kirmizi, Gürcan Güleryüz, Hülya Arslan, Fatma Selcen Sakar

Turkish Journal of Botany

We investigated the germination requirements of a rare endemic plant species, Pedicularis olympica Boiss. (Scrophulariaceae), which grows in wet habitats on Uludağ Mountain, Turkey. We studied the effects of scarification, moist chilling (+4 °C) for 15 days, different doses of gibberellic acid (GA_{3}; 100, 150, and 250 ppm) and combined hormone and moist chilling treatments under dark (20 °C) and photoperiod (20/10 °C; 12/12 h, respectively) conditions. GA_{3} was able to break dormancy. The highest germination rate was found in the seeds treated with 250 ppm GA_{3}; 64% of these seeds germinated when treated with moist chilling and incubated in …


Aav Serotype Influences Gene Transfer In Corneal Stroma In Vivo, Ajay Sharma, Jonathan C. K. Tovey, Arkasubhra Ghosh, Rajiv R. Mohan Jan 2010

Aav Serotype Influences Gene Transfer In Corneal Stroma In Vivo, Ajay Sharma, Jonathan C. K. Tovey, Arkasubhra Ghosh, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

This study evaluated the cellular tropism and relative transduction efficiency of three AAV serotypes, AAV6, AAV8 and AAV9, for corneal gene delivery using mouse cornea in vivo and donor human cornea ex vivo. The AAV6, AAV8 and AAV9 serotypes having AAV2 plasmid encoding for alkaline phosphatase (AP) gene were generated by transfecting HEK293 cell line with pHelper, pARAP4 and pRep/Cap plasmids. Viral vectors (109 vg/μl) were topically applied onto mouse cornea in vivo and human cornea ex vivo after removing the epithelium. Human corneas were processed for transgene delivery at day 5 after viral vector application. Mouse corneas were harvested …