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Cross-Modal Recognition Of Familiar Conspecifics In Goats, Benjamin J. Pitcher, Elodie F. Briefer, Luigi Baciadonna, Alan G. Mcelligott Jan 2017

Cross-Modal Recognition Of Familiar Conspecifics In Goats, Benjamin J. Pitcher, Elodie F. Briefer, Luigi Baciadonna, Alan G. Mcelligott

Recognition Collection

When identifying other individuals, animals may match current cues with stored information about that individual from the same sensory modality. Animals may also be able to combine current information with previously acquired information from other sensory modalities, indicating that they possess complex cognitive templates of individuals that are independent of modality. We investigated whether goats (Capra hircus) possess cross-modal representations (auditory–visual) of conspecifics. We presented subjects with recorded conspecific calls broadcast equidistant between two individuals, one of which was the caller. We found that, when presented with a stablemate and another herd member, goats looked towards the caller sooner and …


Hosts, Nesting Behavior, And Ecology Of Some North American Spider Wasps (Hymenoptera: Pompilidae), Ii, Frank E. Kurczewski, Glavis B. Edwards, James P. Pitts Jan 2017

Hosts, Nesting Behavior, And Ecology Of Some North American Spider Wasps (Hymenoptera: Pompilidae), Ii, Frank E. Kurczewski, Glavis B. Edwards, James P. Pitts

Biology Faculty Publications

This monograph is a continuation of a multiyear study of the genera and species of North American spider wasps (Pompilidae) and their spider host families, genera and species, nesting behavior, ecology, and natural communities. The study enlarges and enhances host preference, nesting behavior, and ecological information for 77 North American pompilid taxa. The first North American Pompilidae records for the families Tengellidae and Segestriidae, both from central coastal California, are reported herein. New host spider genera and species are listed for many of the pompilid species, including the first North American host record for Dipogon (Dipogon) g. graenicheri …


Chapter 2 - Protozoa, Janice M. Glime Jan 2017

Chapter 2 - Protozoa, Janice M. Glime

Bryophyte Ecology Volume 2: Bryological Interaction

Explore the contents of Bryophyte Ecology, Volume 2, Chapter 2 - Protozoa by clicking the links above. For the Contents section of this ebook, as well as Volumes 1 through 5, please visit the Bryophyte Ecology Main Page.

Use CTRL+F to easily search within PDF files.

Ebook sponsored by Michigan Technological University and the International Association of Bryologists.


Chapter 13 - Fish, Janice M. Glime Jan 2017

Chapter 13 - Fish, Janice M. Glime

Bryophyte Ecology Volume 2: Bryological Interaction

Explore the contents of Bryophyte Ecology, Volume 2, Chapter 13 - Fish by clicking the link above. For the Contents section of this ebook, as well as Volumes 1 through 5, please visit the Bryophyte Ecology Main Page.

Use CTRL+F to easily search within PDF files.

Ebook sponsored by Michigan Technological University and the International Association of Bryologists.


Chapter 1 - Introduction, Janice M. Glime Jan 2017

Chapter 1 - Introduction, Janice M. Glime

Bryophyte Ecology Volume 1: Physiological Ecology

Explore the contents of Bryophyte Ecology, Volume 1, Chapter 1 - Introduction by clicking the link above. For the Contents section of this ebook, as well as Volumes 1 through 5, please visit the Bryophyte Ecology Main Page.

Use CTRL+F to easily search within PDF files.

Ebook sponsored by Michigan Technological University and the International Association of Bryologists.


Volume 1, Chapter 4-5: Adaptive Strategies: Growth And Life Forms, Janice M. Glime Jan 2017

Volume 1, Chapter 4-5: Adaptive Strategies: Growth And Life Forms, Janice M. Glime

Bryophyte Ecology Subchapters

No abstract provided.


Responses To Prescribed Fire At Big Thicket National Preserve, Texas, Usa, B. P. Oswald, D. M. Boensch, H. M. Williams, I-Kuai Hung Jan 2017

Responses To Prescribed Fire At Big Thicket National Preserve, Texas, Usa, B. P. Oswald, D. M. Boensch, H. M. Williams, I-Kuai Hung

Faculty Publications

US Federal land managers have utilized hand ignited prescribed fire at Big Thicket National Preserve in efforts to restore the structure and diversity of the longleaf pine (Pinus palustris) forest. A fire ecology study was initiated by Rice University in the early 1990’s and the National Park Service has continued monitoring the plots. Ordination was applied to species abundance data to examine changes in vegetation communities from a variety of prescribed fire treatments and controls. The vegetation data was separated by size class to include overstory, small tree, large sapling and seedling data. Across the size classes and treatments, the …


Feeding Selectivity Of An Algivore (Tropheus Brichardi) In Lake Tanganyika, Robin Richardson-Coy Jan 2017

Feeding Selectivity Of An Algivore (Tropheus Brichardi) In Lake Tanganyika, Robin Richardson-Coy

Browse all Theses and Dissertations

Algivorous fish remove attached algae (periphyton) from the benthos in near shore areas of lakes. Periphyton has a complex three-dimensional structure dominated by Bacillariophyta (diatoms), Chlorophyta (green algae), and Cyanophyta (cyanobacteria). These three phyla vary in nutritional quality with diatoms providing essential fatty acids that consumers need for growth and reproduction. Selection of specific phyla may be driven by nutritional quality or it may be a function accessibility due to both mouth morphology of the fish and location of the algae in the periphyton community. I investigated whether Tropheus brichardi, an algivorous cichlid of Lake Tanganyika, selectivity feeds on …


Functional Traits Of Expanding, Thicket-Forming Shrubs: Contrasting Strategies Between Exotic And Native Species, Sheri A. Shiflett, Julie C. Zinnert, Donald R. Young Jan 2017

Functional Traits Of Expanding, Thicket-Forming Shrubs: Contrasting Strategies Between Exotic And Native Species, Sheri A. Shiflett, Julie C. Zinnert, Donald R. Young

Biology Publications

Woody expansion has been documented for decades in many different systems globally, often yielding vast changes in ecosystem functioning. While causes and consequences of woody expansion have been well documented, few studies have addressed plant functional traits that promote dramatic and rapid expansion in range. Our objectives were to investigate plant functional traits that contribute to the colonization, rapid expansion, and thicket formation of an invasive, N-fixing shrub, Elaeagnus umbellata Thunb. (Elaeagnaceae), and a native, N-fixing shrub Morella cerifera (L.) Small (Myricaceae) and compare to native, sympatric, non-expanding shrub species. Quantified functional traits included morphological (e.g., specific leaf area, leaf …


Nuclear Genome Size Diversity Of Marine Invertebrate Taxa Using Flow Cytometric Analysis, Kyle Roebuck Jan 2017

Nuclear Genome Size Diversity Of Marine Invertebrate Taxa Using Flow Cytometric Analysis, Kyle Roebuck

Lopez Laboratory: Genomics & Microbial Data

No abstract provided.


The Effect Of Site Characteristics On The Reproductive Output Of Lesser Celandine (Ranunculus Ficaria), Justin P. Kermack Jan 2017

The Effect Of Site Characteristics On The Reproductive Output Of Lesser Celandine (Ranunculus Ficaria), Justin P. Kermack

ETD Archive

Ranunculus ficaria L., an ephemeral perennial invasive plant brought over from Europe, is becoming widespread throughout the Northeastern United States. This herbaceous buttercup is able to create extensive dense mats that limit native species growth. Taking advantage of an early growing season and rapid reproduction rates, this species can create dense monocultures, which threatens native communities and ecosystems. Elimination of native spring ephemerals results in decreased biodiversity. A better understanding of how R. ficaria responds to site characteristics is needed to prioritize management efforts toward high-risk sites.

Ranunculus ficaria abundance and reproductive output (seed, bulbil and tuber production rates) were …


The Feasibility Of Counting Songbirds Using Unmanned Aerial Vehicles, Andrew M. Wilson, Janine M. Barr, Megan E. Zagorski Jan 2017

The Feasibility Of Counting Songbirds Using Unmanned Aerial Vehicles, Andrew M. Wilson, Janine M. Barr, Megan E. Zagorski

Environmental Studies Faculty Publications

Obtaining unbiased survey data for vocal bird species is inherently challenging due to observer biases, habitat coverage biases, and logistical constraints. We propose that combining bioacoustic monitoring with unmanned aerial vehicle (UAV) technology could reduce some of these biases and allow bird surveys to be conducted in less accessible areas. We tested the feasibility of the UAV approach to songbird surveys using a low-cost quadcopter with a simple, lightweight recorder suspended 8 m below the vehicle. In a field experiment using playback of bird recordings, we found that small variations in UAV altitude (it hovered at 28, 48, and 68 …


Climate Dynamics, Invader Fitness, And Ecosystem Resistance In An Invasion-Factor Framework, Stephen L. Young, David R. Clements, Antonio Ditommaso Jan 2017

Climate Dynamics, Invader Fitness, And Ecosystem Resistance In An Invasion-Factor Framework, Stephen L. Young, David R. Clements, Antonio Ditommaso

West Central Research and Extension Center, North Platte

As researchers and land managers increasingly seek to understand plant invasions and the external (climate) and internal (plant genetics) conditions that govern the process, new insight is helping to answer the elusive question of what makes some invasions successful and others not. Plant invasion success or failure is based on a combination of evolutionary and ecological processes. Abiotic (e.g., climate) and biotic (e.g., plant competition) conditions in the environment and plant genetics (e.g., fitness) combine in either decreasing or increasing invasion, yet it has proven challenging to know exactly which of these conditions leads to success for a given species, …


Wild Stimulant Production Methods For American Ginseng Farms In Tennessee, Shannon Smith, John Dubois, Nate Phillips, Arvazena Clardy Jan 2017

Wild Stimulant Production Methods For American Ginseng Farms In Tennessee, Shannon Smith, John Dubois, Nate Phillips, Arvazena Clardy

Extension Publications

No abstract provided.


Floating Algae Blooms In The East China Sea, Lin Qi, Chuanmin Hu, Mengqiu Wang, Shaoling Shang, Cara Wilson Jan 2017

Floating Algae Blooms In The East China Sea, Lin Qi, Chuanmin Hu, Mengqiu Wang, Shaoling Shang, Cara Wilson

Marine Science Faculty Publications

A floating algae bloom in the East China Sea was observed in Moderate Resolution Imaging Spectroradiometer (MODIS) imagery in May 2017. Using satellite imagery from MODIS, Visible Infrared Imaging Radiometer Suite, Geostationary Ocean Color Imager, and Ocean Land Imager, and combined with numerical particle tracing experiments and laboratory experiments, we examined the history of this bloom as well as similar blooms in previous years and attempted to trace the bloom source and identify the algae type. Results suggest that one bloom origin is offshore Zhejiang coast where algae slicks have appeared in satellite imagery almost every February–March since 2012. Following …


Population Structure And Genetic Diversity Of Bromus Tectorum Within The Small Grain Production Region Of The Pacific Northwest, Nevin C. Lawrence, Amber L. Hauvermale, Amit Dhingra, Ian C. Burke Jan 2017

Population Structure And Genetic Diversity Of Bromus Tectorum Within The Small Grain Production Region Of The Pacific Northwest, Nevin C. Lawrence, Amber L. Hauvermale, Amit Dhingra, Ian C. Burke

Department of Agronomy and Horticulture: Faculty Publications

Bromus tectorum L. is an invasive winter annual grass naturalized across the United States. Numerous studies have investigated B. tectorum population structure and genetics in the context of B. tectorum as an ecological invader of natural areas and rangeland. Despite the wealth of information regarding B. tectorum, previous studies have not focused on, or made comparisons to, B. tectorum as it persists in individual agroecosystems. The objectives of this study were to assess the genetic diversity and structure, the occurrence of generalist and specialist genotypes, and the influence of climate on distribution of B. tectorum sourced exclusively from within …


A New Tachinid Genus And Species Record For North America: Iceliopsis Borgmeieri Guimarães, John O. Stireman Iii, Jane E. Dell Jan 2017

A New Tachinid Genus And Species Record For North America: Iceliopsis Borgmeieri Guimarães, John O. Stireman Iii, Jane E. Dell

Biological Sciences Faculty Publications

The Iceliini are a small, enigmatic New World tribe of Tachininae consisting of three genera, Icelia RobineauDesvoidy, Iceliopsis Guimarães, and Erviopsis Townsend, and five recognized species (Guimarães 1976). All known species are exclusively Neotropical in distribution with the exception of Icelia triquetra (Olivier), which ranges from Brazil, through Central America, and as far north as New York state (O’Hara & Wood 2004). Members of the tribe are generally medium-sized (ca. 7–12 mm), elongate, yellowish or grayish in color, and resemble Dexiini or Leskiini in general appearance. Species of Iceliini are relatively rarely collected and there is but a single host …


Remote Estimation Of Biomass Of Ulva Prolifera Macroalgae In The Yellow Sea, Lianbo Hu, Chuanmin Hu, He Ming-Xia Jan 2017

Remote Estimation Of Biomass Of Ulva Prolifera Macroalgae In The Yellow Sea, Lianbo Hu, Chuanmin Hu, He Ming-Xia

Marine Science Faculty Publications

Since 2008, macroalgal blooms of Ulva prolifera (also called green tides) occurred every summer in the Yellow Sea (YS), causing environmental and economic problems. A number of studies have used satellite observations to estimate the severity of the blooms through estimating the bloom size and duration. However, a critical bloom parameter, namely biomass, has never been objectively determined due to lack of measurements. In this study, laboratory experiments were conducted to measure U. prolifera biomass (wet weight) per unit area and the corresponding spectral reflectance, through which a robust relationship has been established to link biomass per area to …


Identification Of Candidate Cellulose Utilizing Bacteria From The Rumen Of Beef Cattle, Using Bacterial Community Profiling And Metagenomics, Lee Opdahl Jan 2017

Identification Of Candidate Cellulose Utilizing Bacteria From The Rumen Of Beef Cattle, Using Bacterial Community Profiling And Metagenomics, Lee Opdahl

Electronic Theses and Dissertations

The ruminal microbiome allows ruminant animals to convert cellulosic biomass into food products. A majority of ruminal microorganisms remain uncharacterized due, in part, to the complexity of ruminal microbial communities. In order to gain further insight, selection-based batch culturing from bovine rumen fluid, in combination with metagenomics, was used to identify and characterize previously uncharacterized rumen bacteria capable of metabolizing cellulose, which was supplemented as a purified substrate. 16S rRNA-based population analysis was used to identify rumen bacteria enriched within 14 days of culturing. As a result of 4 independent experiments, seven different candidate cellulose-utilizing species-level operational taxonomic units (OTUs) …


Measuring Aquatic Organism Responses To Grassland Restoration: Does The Field Of Dreams Really Exist?, David A. Schumann Jan 2017

Measuring Aquatic Organism Responses To Grassland Restoration: Does The Field Of Dreams Really Exist?, David A. Schumann

Electronic Theses and Dissertations

Landscape homogenization and the degradation of riparian areas has greatly impaired stream ecosystems throughout North America. Conservation programs may repair riparian ecosystems to indirectly improve water quality and instream habitat heterogeneity in hopes to elicit biological responses. However, focused manipulations on isolated stream fragments have rarely achieved biological goals. Prairie streams with restored riparian areas were appraised (chemical, physical, and biological variables) to quantify the indirect effects of prevalent grassland conservation practices on aquatic resources. Riparian rehabilitation, via passive methods, promoted bank-stabilizing vegetation along all conservation stream reaches. Riparian vegetation and function quickly recovered from previous agricultural disturbances at conservation …


Ecology Of Blanchard Springs Caverns, Ozark National Forest, Arkansas: 42 Years Later, C. J. Midden, S. K. Sasser, J. L. Grove Jan 2017

Ecology Of Blanchard Springs Caverns, Ozark National Forest, Arkansas: 42 Years Later, C. J. Midden, S. K. Sasser, J. L. Grove

Journal of the Arkansas Academy of Science

Interrelationships between subterranean and epigean environments affect dispersion and distribution of cave organisms among the macro and microhabitats. This study examined the environmental impact of 42 years of tourism and development in the two lower sections of Blanchard Springs Caverns found in Stone County, Arkansas; and contributes to a better understanding of the seasonal fluctuations of the abiotic and biotic parameters.

Temperature, water quality, and fauna data were collected. A new entrance, lighting, and approximately 12,500 visitors during the 12-month study had no observable effect on cavern temperatures. Stream water quality measurements were comparable to Grove’s 1974 study. Gray bat, …


Species Diversity And Community Structure Of Zooplankton In Threedifferent Types Of Water Body Within The Sakarya River Basin, Turkey, Pinar Gürbüzer, Özge Buyurgan, Çağri Tekatli, Ahmet Altindağ Jan 2017

Species Diversity And Community Structure Of Zooplankton In Threedifferent Types Of Water Body Within The Sakarya River Basin, Turkey, Pinar Gürbüzer, Özge Buyurgan, Çağri Tekatli, Ahmet Altindağ

Turkish Journal of Zoology

The aim of this study was to identify differences between different types of water body within the same river basin by comparing the composition of the zooplankton community. The study was conducted in three types of water body in the Sakarya Basin in northwestern Turkey: an alluvial barrier lake (Lake Poyrazlar), a pond (Sorgun Pond), and a reservoir (Çubuk II Reservoir). The spatiotemporal changes of these three types of aquatic habitats were investigated in terms of the zooplankton organisms by examining their diversity, evenness, and differences in the abundance of species. A total of 81 species were identified. Thirty-eight species …


Balancing Stability And Flexibility In Adaptive Governance: An Analysis Of Tools Available In U.S. Environmental Law, Robin Kundis Craig, Ahjond S. Garmestani, Craig R. Allen, Craig Anthony (Tony) Arnold, Hannah E. Birge, Daniel A. Decaro, Alexander K. Fremier, Hannah Gosnell, Edella Schlager Jan 2017

Balancing Stability And Flexibility In Adaptive Governance: An Analysis Of Tools Available In U.S. Environmental Law, Robin Kundis Craig, Ahjond S. Garmestani, Craig R. Allen, Craig Anthony (Tony) Arnold, Hannah E. Birge, Daniel A. Decaro, Alexander K. Fremier, Hannah Gosnell, Edella Schlager

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Adaptive governance must work “on the ground,” that is, it must operate through structures and procedures that the people it governs perceive to be legitimate and fair, as well as incorporating processes and substantive goals that are effective in allowing social-ecological systems (SESs) to adapt to climate change and other impacts. To address the continuing and accelerating alterations that climate change is bringing to SESs, adaptive governance generally will require more flexibility than prior governance institutions have often allowed. However, to function as good governance, adaptive governance must pay real attention to the problem of how to balance this increased …


A Bayesian Method For Assessing Multi-Scale Species-Habitat Relationships, Erica F. Stuber, Lutz F. Gruber, Joseph J. Fontaine Jan 2017

A Bayesian Method For Assessing Multi-Scale Species-Habitat Relationships, Erica F. Stuber, Lutz F. Gruber, Joseph J. Fontaine

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Context Scientists face several theoretical and methodological challenges in appropriately describing fundamental wildlife-habitat relationships in models. The spatial scales of habitat relationships are often unknown, and are expected to follow a multi-scale hierarchy. Typical frequentist or information theoretic approaches often suffer under collinearity in multiscale studies, fail to converge when models are complex or represent an intractable computational burden when candidate model sets are large.

Objectives Our objective was to implement an automated, Bayesian method for inference on the spatial scales of habitat variables that best predict animal abundance.

Methods We introduce Bayesian latent indicator scale selection (BLISS), a Bayesian …


Taxonomic Position Of Abies Equi-Trojani On The Basis Of Needlecharacters By Comparison With Different Fir Species, Anna Katarzyna Jasinska, Katarzyna Sekiewicz, Tolga Ok, Angel Romo, Adam Boratynski, Krystyna Boratynska Jan 2017

Taxonomic Position Of Abies Equi-Trojani On The Basis Of Needlecharacters By Comparison With Different Fir Species, Anna Katarzyna Jasinska, Katarzyna Sekiewicz, Tolga Ok, Angel Romo, Adam Boratynski, Krystyna Boratynska

Turkish Journal of Botany

The needles from 25 trees of Abies equi-trojani from Kazdağı (Mount Ida) were compared with those from four populations of A. alba, two of A. cephalonica, and three of each of A. xborisii-regis, A. bornmuelleriana, and A. nordmanniana, each represented by 29-31 individuals. Thirty-nine morphological and anatomical needle characters (26 main and 13 estimated ones) were examined biometrically to verify the systematic position of A. equi-trojani. To evaluate the relationships among taxa, Student's t-test for independent samples, the Mann-Whitney U test, and discrimination and clustering analyses were applied. The differences between taxa were statistically significant for several characters, but the …


Exploring Predator-Prey Interactions In Agroecosystems Through Molecular Gut-Content Analysis, Kacie J. Athey Jan 2017

Exploring Predator-Prey Interactions In Agroecosystems Through Molecular Gut-Content Analysis, Kacie J. Athey

Theses and Dissertations--Entomology

Generalist predators can contribute to vital ecosystem services by potentially inducing trophic cascades as natural enemies of pests in agroecosystems. As the human population of the world gets larger, we need to produce more food on ever-smaller swaths of available land relying on ecosystem services, in the form of pest control, that may contribute to agricultural sustainability. Teasing apart the exact trophic linkages between predators and prey is a vital first step and essential to uncovering which predators are inducing trophic cascades and should be enhanced through conservation biological control.

Combined with ecological experimentation, the main tool used throughout my …


Swift Foxes In Southwestern South Dakota: Assessing The Current Status Of A Reintroduced Population, Sarah Ann Nevison Jan 2017

Swift Foxes In Southwestern South Dakota: Assessing The Current Status Of A Reintroduced Population, Sarah Ann Nevison

Electronic Theses and Dissertations

Swift foxes (Vulpes velox) were reintroduced into Badlands National Park between 2003 and 2006 after being nearly extirpated from South Dakota in the early 1900’s. Genetic analysis provided strong evidence that the reintroduction was successful, but viability analysis indicated the population may be in jeopardy with a high probability of extinction. Recently, the population has declined due to various biotic and abiotic factors (e.g., recent weather patterns, effects of plague [Yersinia pestis], and increased coyote [Canis latrans] numbers). No information on the status of swift foxes has been collected since 2009. Between 2014 and 2016, the objectives of this study …


Evaluating The Influence Of Water Developments On The Demography And Spatial Ecology Of A Rare, Desert-Adapted Carnivore: The Kit Fox (Vulpes Macrotis), Bryan M. Kluever, Eric M. Gese Jan 2017

Evaluating The Influence Of Water Developments On The Demography And Spatial Ecology Of A Rare, Desert-Adapted Carnivore: The Kit Fox (Vulpes Macrotis), Bryan M. Kluever, Eric M. Gese

United States Department of Agriculture Wildlife Services: Staff Publications

Constructing water developments to support anthropogenic activities and particular fauna is pervasive across many arid regions of the globe. Despite their prevalence and a predicted increase as a management and conservation tool, water developments may have complex and unanticipated impacts on wildlife. For example, the addition of water developments to the Great Basin Desert in the western United States may have indirectly contributed to a decrease in distribution and abundance of kit foxes (Vulpes macrotis). From 2010 to 2013, we examined survival, relative abundance, and habitat characteristics of kit foxes in relation to water developments on the U.S. …


The Digestive Composition And Physiology Of Water Mites, Adrian Amelio Vasquez Jan 2017

The Digestive Composition And Physiology Of Water Mites, Adrian Amelio Vasquez

Wayne State University Dissertations

Water mites are a diverse group of arachnids that inhabit aquatic habitats and have been studied in the past for their biodiversity, unique lifecycle, bioindicator species use and for their impact as parasites on insects of human pathological significance such as the mosquito. Water mites are critical in their environment as possible apex predators however, their life cycle and morphological complexity has made taxonomy and description of water mites difficult. Although water mite species richness is estimated at over 6000 species described to date, descriptions of extant North American water mite species are estimated to be only 50% of the …


Are Mussel Shells Environmental Dna Time Capsules? A Comparison Of Extraction Methods For Obtaining Dna From Shell Material., Kelsey Elizabeth Rogers Jan 2017

Are Mussel Shells Environmental Dna Time Capsules? A Comparison Of Extraction Methods For Obtaining Dna From Shell Material., Kelsey Elizabeth Rogers

Online Theses and Dissertations

Freshwater mussels have become some of the most imperiled species in North America and widespread populations have succumbed to pollution and many other anthropogenic-related factors. With molecular techniques evolving, a recent interest in ancient DNA and museum specimens has emerged and prompted a study to test the ability of several extraction methods to isolate DNA from museum mussel specimens. The purpose of this study was to determine if four DNA extraction methods had influence on total DNA yield (ng/mg) from mussel tissue. The hinge ligaments of freshwater mussels ranging in collection date (1984-2015) were used as the source of genetic …