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Tannerella Forsythia, A Periodontal Pathogen Entering The Genomic Era, Anne C. R. Tanner, Jacques Izard Jan 2006

Tannerella Forsythia, A Periodontal Pathogen Entering The Genomic Era, Anne C. R. Tanner, Jacques Izard

Department of Food Science and Technology: Faculty Publications

Several questions need to be addressed to evaluate whether Tannerella forsythia is to be considered a periodontal pathogen. T. forsythia has been detected in periodontal health and disease, so could it be a pathogen? The species was not detected in many studies despite finding other putative pathogens, so could it be important in pathogenicity? The challenges of working with T. forsythia include its fastidious and anaerobic growth requirements for cultural detection. Thus, studies associating T. forsythia with periodontal and other oral infections have used noncultural approaches (immunoassays and DNA-based assays) in addition to cultural approaches. We feel …

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Expression Of Apoe In The Mouse Olfactory System During Development And Post Injury, Sreenivas Nannapaneni Jan 2006

Expression Of Apoe In The Mouse Olfactory System During Development And Post Injury, Sreenivas Nannapaneni

Masters Theses

Apolipoprotein E (apoE), a prominent player in transporting cholesterol and metabolizing plasma lipoproteins, has emerged as a major risk factor in causing Alzheimer's disease (AD). ApoE, extensively expressed in the primary olfactory pathway, binds to its low-density lipoprotein receptor and related receptors to play a role both in development and in control of the immune system. Previous studies have shown that apoE is expressed at high concentrations in the olfactory nerve (ON) and around the glomeruli of the olfactory bulb (OB) and that the apoE levels in these regions increase substantially following lesioning of the olfactory epithelium (OE). Studies from …

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Gamete And Cell Technologies For Use In Biological Resource Banking, Liesl Nel-Themaat Jan 2006

Gamete And Cell Technologies For Use In Biological Resource Banking, Liesl Nel-Themaat

LSU Doctoral Dissertations

Biological resource banking is becoming important for endangered species conservation. A series of experiments were conducted to address issues concerning collection and utilization of biomaterials from Gulf Coast Native (GCN) sheep (model species) (Ovis aries) and Eland antelope (Taurotragus oryx). In the first experiment, two ejaculates were collected 10 minutes apart from each of five rams three times a week for three weeks to maximize output and minimize handling time. Semen volume, concentration and total number of spermatozoa were significantly greater in first ejaculates, whereas pre-cooled, cooled and post-thaw motility, as well as sperm survival, were greater in second ejaculates. …

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College Student's Motivation For Physical Activity, Lori Lynn Delong Jan 2006

College Student's Motivation For Physical Activity, Lori Lynn Delong

LSU Doctoral Dissertations

The purpose of this study was to examine college students' motivations to be physically active by merging the perspectives of self-determination theory and the transtheoretical model. The secondary purpose was to examine the effects of a required physical activity course on college students' levels of physical activity. The premise is that both the theory and model can be used to help predict physical activity outcomes. Participants were 277 male and female students at a small private college. Motivation, self-determination, stage of change, self-efficacy, decisional balance, and leisure time activity levels were assessed using an online survey. Results revealed that activity …

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Nitrogen Fixation And Release Of Fixed Nitrogen By Trichodesmium Spp. In The Gulf Of Mexico, Margaret R. Mulholland, Peter W. Bernhardt, Cynthia A. Heil, Deborah A. Bronk, Judith M. O'Neil Jan 2006

Nitrogen Fixation And Release Of Fixed Nitrogen By Trichodesmium Spp. In The Gulf Of Mexico, Margaret R. Mulholland, Peter W. Bernhardt, Cynthia A. Heil, Deborah A. Bronk, Judith M. O'Neil

OES Faculty Publications

During a 3-yr study in the Gulf of Mexico, we measured dinitrogen (N2) fixation and nitrogen (N) release by Trichodesmium and compared these rates with water column N demand and the estimated N necessary to support blooms of Karenia brevis, a toxic dinoflagellate that severely affects the West Florida shelf. Net and gross N2 fixation rates were compared in simultaneous incubations using 15N2 uptake and acetylene reduction, respectively. The difference between net and gross N2 fixation is assumed to be an approximation of the rate of N release. Results demonstrate that Trichodesmium in …

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Effects Of Commercial Diazinon And Imidacloprid On Microbial Urease Activity In Soil And Sod, C. W. Ingram, Mark S. Coyne, David W. Williams Sep 2005

Effects Of Commercial Diazinon And Imidacloprid On Microbial Urease Activity In Soil And Sod, C. W. Ingram, Mark S. Coyne, David W. Williams

Plant and Soil Sciences Faculty Publications

Diazinon [O,O-diethyl O-2-isopropyl-6-methyl(pyrimidine-4-yl) phosphorothioate] and imidacloprid [1-(1-[6-chloro-3-pyridinyl]methyl)-N-nitro-2-imidazolidinimine] are applied to lawns for insect control simultaneously with nitrogenous fertilizers such as urea, but their potential effect on urease activity and nitrogen availability in turfgrass management has not been evaluated. Urease activity in enzyme assays, washed cell assays, and soil slurries was examined as a function of insecticide concentration. Intact cores from field sites were used to assess the effect of insecticide application on urease activity in creeping bentgrass (Agrostis palustris Huds.) and bluegrass (Poa pratensis L.) sod. Bacterial urease fromBacillus pasteurii and plant …

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Interpretive Signs Designed To Trigger Naturalist Intelligence At Two American Zoos, Martha Bryant Aug 2005

Interpretive Signs Designed To Trigger Naturalist Intelligence At Two American Zoos, Martha Bryant

Graduate Student Dissertations, Theses, Capstones, and Portfolios

An investigation of interpretive graphics was conducted in 2005 at two mid-sized AZA-accredited zoos, Lowry Park Zoo, Tampa, Florida and Knoxville Zoo, Knoxville, Tennessee. The Lowry Park Zoo study investigated signs at a red-tailed hawk and sandhill crane exhibit. Combination signs and wordless signs were more effective helping visitors see animals, increasing holding time, and number of engagements than treatments of no signs, or signs with words only. A second study, at Knoxde Zoo, tested combination and wordless signs in a children's zoo, investigating 31 signs at a 3.5-acre exhibit. Comparisons of visitors seeing the animals/using interactive exhibit elements, holding …

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Sheep Updates 2005 - Part 3, Rob Davidson, David Pethick, C. F. Engelke, B. D. Siebert, K. Gregg, A-D G. Wright, P. E. Vercoe, Robin Jacob, Russell Barnett, Joanne Sneddon, Sandra Brown, G. Rose, C. Kabore, J Dart, Stuart Adams Jul 2005

Sheep Updates 2005 - Part 3, Rob Davidson, David Pethick, C. F. Engelke, B. D. Siebert, K. Gregg, A-D G. Wright, P. E. Vercoe, Robin Jacob, Russell Barnett, Joanne Sneddon, Sandra Brown, G. Rose, C. Kabore, J Dart, Stuart Adams

Sheep Updates

This session covers seven papers from different authors: CUSTOMER 1. Benefits VIAscanR to producers and WAMMCO, Rob Davidson, Supply Development Manager, David Pethick, School of Veterinary and Biomedical Studies, Murdock University. 2. Healthy fats in lamb: how WA lambs compare with others, C. F. Engelke Animal Biology, University of Western Australia, bCSIRO Livestock Industries, Western Australia B.D. Siebert, Department of Animal Science, University of Adelaide, South Australia, K. Gregg, Centre for High-Throughput Agricultural Genetic Analysis, Murdoch University, Western Australia. A-D.G. Wright CSIRO Livestock Industries, Western Australia, P.E Vercoe Animal Biology, University of Western Australia 3. Shelf life of fresh lamb …

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Time And Frequency Of Applications Of Entomopathogenic Nematodes And Their Persistence For Control Of Western Flower Thrips Frankliniella Occidentalis, Difabachew Belay, Lemma Ebssa, Christian Borgemeister Jul 2005

Time And Frequency Of Applications Of Entomopathogenic Nematodes And Their Persistence For Control Of Western Flower Thrips Frankliniella Occidentalis, Difabachew Belay, Lemma Ebssa, Christian Borgemeister

All Peer-Reviewed Publications

Post application persistence of two entomopathogenic nematode (EPN) species, Heterorhabditis bacteriophora strain HK3 and Steinernema carpocapsae strain DD136, was studied against soil-dwelling late second instar larvae (L2) of western flower thrips (WFT) Frankliniella occidentalis (Thysanoptera: Thripidae). The nematodes were applied at 200 and 400 infective juveniles (IJ) cm-2, and L2 WFT were introduced at 0 (same day), 3, 6, 9 or 12 days after nematode application (DANA). Heterorhabditis bacteriophora caused higher thrips mortality than S. carpocapsae in most of the tests and both species persisted for at least 6 days, causing WFT mortality of up to 76 and 37.8%, respectively. …

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Rhabditis (Oscheius) Colombiana N. Sp. (Nematoda: Rhabditidae), A Necromenic Associate Of The Subterranean Burrower Bug Cyrtomenus Bergi (Hemiptera: Cydnidae) From The Cauca Valley, Colombia, S. Patricia Stock, Ana M. Caicedo, Paul A. Calatayud May 2005

Rhabditis (Oscheius) Colombiana N. Sp. (Nematoda: Rhabditidae), A Necromenic Associate Of The Subterranean Burrower Bug Cyrtomenus Bergi (Hemiptera: Cydnidae) From The Cauca Valley, Colombia, S. Patricia Stock, Ana M. Caicedo, Paul A. Calatayud

All Peer-Reviewed Publications

Rhabditis (Oscheius) colombiana n. sp. is described as a necromenic associate of the burrower bug Cyrtomenus bergi (Hemiptera: Cydnidae) collected in the Cauca Valley, Colombia. The new species resembles others of the insectivora-group, viz, Rhabditis (O.) insectivora Körner, 1954 and Rhabditis (O.) lucianii Maupas, 1919, in a number of morphological and life history traits, but can be separated by a combination of morphological and morphometric characters. The most distinctive morphological features that separate R. (O.) colombiana n. sp. from these two Rhabditis species are the total size of both males and females, which are much shorter and slimmer in the …

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Aging Affects Stretch-Induced P70s6k And 4e-Bp1 Phosphorylation In Fast- And Slow-Twitch Muscle, Sreevani Uddemarri Jan 2005

Aging Affects Stretch-Induced P70s6k And 4e-Bp1 Phosphorylation In Fast- And Slow-Twitch Muscle, Sreevani Uddemarri

Theses, Dissertations and Capstones

In the present investigation we compare the expression, basal activation and the ability of muscle stretch to activate the p70S6k pathway in the fast-twitch extensor digitorum longus (EDL) and slow-twitch soleus of adult (6 mo. old), aged (30 mo. old) and very aged (36 mo. old) Fischer 344 x Brown Norway rats. Immunoblotting demonstrated that the tissue content of mTOR, p70S6k, 4E-BP1 and GSK-3β decreased in the EDL and soleus in aged rats, while SHP-2 increased in the EDL and decreased in the soleus when compared to adult rats. Basal phosphorylation of 4E-BP1 increased in both the …

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Selenotrisulfide Derivative Of Alpha-Lipoic Acid: Evaluation In A Cell Culture Model For Potential Use As A Topical Antioxidant, Melenie Lee Alonis Jan 2005

Selenotrisulfide Derivative Of Alpha-Lipoic Acid: Evaluation In A Cell Culture Model For Potential Use As A Topical Antioxidant, Melenie Lee Alonis

Electronic Theses and Dissertations

Selenium is a required micronutrient in mammalian cells. It is incorporated in the form of selenocysteine into selenoenzymes such as glutathione peroxidase and thioredoxin reductase, and is absolutely required for activity. Thioredoxin reductase is necessary for reduction of oxidized thioredoxin and therefore plays a major role in maintaining the redox status of the cell. Glutathione peroxidase is responsible for reducing peroxides into their corresponding alcohols and water. Together, these selenoenzymes constitute a significant part of the cell's arsenal to defend itself against oxidative stress. Exogenous sources of oxidative stress, such as UV radiation, are capable of generating reactive oxygen species …

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Fecycle: Attempting An Iron Biogeochemical Budget From A Mesoscale Sf6 Tracer Experiment In Unperturbed Low Iron Waters, Robert Michael Mckay, P. W. Boyd, C. S. Law, D. A. Hutchins, E. R. Abraham, P. L. Croot, M. Ellwood, R. D. Frew, M. Hadfield, J. Hall, S. Handy, C. Hare, J. Higgins, P. Hill, K. A. Hunter, K. Leblanc, M. T. Maldonado, C. Mioni, M. Oliver, S. Pickmere, M. Pinkerton, K. Safi, S. Sander, S. A. Sanudo-Wilhelmy, M. Smith, R. Strzepek, A. Tovar-Sanchez, S. W. Wilhelm Jan 2005

Fecycle: Attempting An Iron Biogeochemical Budget From A Mesoscale Sf6 Tracer Experiment In Unperturbed Low Iron Waters, Robert Michael Mckay, P. W. Boyd, C. S. Law, D. A. Hutchins, E. R. Abraham, P. L. Croot, M. Ellwood, R. D. Frew, M. Hadfield, J. Hall, S. Handy, C. Hare, J. Higgins, P. Hill, K. A. Hunter, K. Leblanc, M. T. Maldonado, C. Mioni, M. Oliver, S. Pickmere, M. Pinkerton, K. Safi, S. Sander, S. A. Sanudo-Wilhelmy, M. Smith, R. Strzepek, A. Tovar-Sanchez, S. W. Wilhelm

Biological Sciences Faculty Publications

An improved knowledge of iron biogeochemistry is needed to better understand key controls on the functioning of high-nitrate low-chlorophyll (HNLC) oceanic regions. Iron budgets for HNLC waters have been constructed using data from disparate sources ranging from laboratory algal cultures to ocean physics. In summer 2003 we conducted FeCycle, a 10-day mesoscale tracer release in HNLC waters SE of New Zealand, and measured concurrently all sources (with the exception of aerosol deposition) to, sinks of iron from, and rates of iron recycling within, the surface mixed layer. A pelagic iron budget (timescale of days) indicated that oceanic supply terms (lateral …

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Locatization Of Human Herpesvirus 8 In Human Sperms By In Situ Pcr, Omar Bagasra, Deepa Patel, Lisa Bobroski, Jamil A. Abbasi, Alex U. Bagasra, Hasna Baidouri, Twaina Harris, Albert El-Roeiy, Zsolt Lengvarszky, Homayoon Farzadegan, Charles Wood Jan 2005

Locatization Of Human Herpesvirus 8 In Human Sperms By In Situ Pcr, Omar Bagasra, Deepa Patel, Lisa Bobroski, Jamil A. Abbasi, Alex U. Bagasra, Hasna Baidouri, Twaina Harris, Albert El-Roeiy, Zsolt Lengvarszky, Homayoon Farzadegan, Charles Wood

Nebraska Center for Virology: Faculty Publications

Objectives: Defining the mechanism of infection with human herpesvirus-8 (HHV-8) or Kaposi’s sarcoma associated herpesvirus (KSHV) is an important clinical issue. HHV-8 has been linked to Kaposi’s sarcoma (KS) development in HIV-1-infected individuals, and KS develops in 40% of those infected with both viruses. A series of epidemiological data suggest that sexual transmission is one of the routes of transmission for HHV-8. In our studies, we sought to assess the cellular reservoirs of HHV-8 DNA in the semen of HIV-1-infected men and the potential role of HHV-8 infected spermatozoa in horizontal transmission. Design and methods: A nested polymerase chain reaction …

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Analytical And Synthetic Methods For Anatoxins., Janet Crowley Jan 2005

Analytical And Synthetic Methods For Anatoxins., Janet Crowley

Theses

This thesis is focused on the potent cyanobacterial neurotoxins anatoxin-a (AN), its methyl analogue homoanatoxin-a (HMAN) and their non-toxic dihydro- and epoxydegradation products. In commencing the investigation into this area a literature review was performed in order to identify areas where more research and method development was required (Chapter 1). The review highlighted the fact that although a number of analytical methods had been developed for the analysis of AN, there were few methods for the determination of its potent homologue HMAN and their non-toxic degradation products. As these neurotoxins degrade quickly it is important to monitor their degradation products, …

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Etiology Of Oral Disease In View Of Microbial Complexity, Anne C. R. Tanner, Jacques Izard Jan 2005

Etiology Of Oral Disease In View Of Microbial Complexity, Anne C. R. Tanner, Jacques Izard

Department of Food Science and Technology: Faculty Publications

The number of different microorganisms recognized in the oral cavity using molecular methods has more than doubled compared with the number isolated using cultural techniques. This finding necessitates a reevaluation of which species may be pathogens in dental infections. Molecular methods used to determine microbial diversity include broad range target Polymerase Chain Reaction with ‘universal primers,’ and cloning amplicons or denaturing gradient gel electrophoresis to separate DNA fragments before sequencing. These molecular methods have clarified and expanded the taxonomy of oral microbial species. Discrepancies between comprehensive molecular and cultural methods suggest that neither method alone can adequately evaluate associations of …

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The Effect Of Growth Rate, Phosphorus Concentration, And Temperature On N-2 Fixation, Carbon Fixation, And Nitrogen Release In Continuous Cultures Of Trichodesmium Ims101, Margaret R. Mulholland, Peter W. Bernhardt Jan 2005

The Effect Of Growth Rate, Phosphorus Concentration, And Temperature On N-2 Fixation, Carbon Fixation, And Nitrogen Release In Continuous Cultures Of Trichodesmium Ims101, Margaret R. Mulholland, Peter W. Bernhardt

OES Faculty Publications

With the use of continuous culture systems, rates of dinitrogen (N2) and carbon (C) fixation and nitrogen (N)- and C-based doubling times were assessed in Trichodesmium IMS101 growing exponentially at steady state dilution rates of 0.10, 0.20, and 0.33 d-1 (doubling times of 10, 5, and 3 d - within the range reported for natural populations). Rates of C fixation, N2 fixation, and N release were examined in replicate culture systems with several techniques. Biomass-specific C uptake varied little with population doubling time, but N2 fixation and N release varied markedly among treatments. Total daily …

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Immunity In Medicine, M Saeed Sep 2004

Immunity In Medicine, M Saeed

Department of Biological & Biomedical Sciences

No abstract provided.

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The Effects Of Selenium On Estrogen-Regulated Gene Expression In Lncap Prostate Cancer Cells, Tory L. Parker Aug 2004

The Effects Of Selenium On Estrogen-Regulated Gene Expression In Lncap Prostate Cancer Cells, Tory L. Parker

Theses and Dissertations

Prostate cancer is the most frequently diagnosed cancer in American men and the second leading cause of cancer deaths. Supplementation with Se has reduced the incidence of prostate cancer and Se status is inversely correlated with prostate cancer risk. One molecular mechanism by which high Se concentrations may affect cancer risk is by catalyzing disulfide bond formation or otherwise complexing with reactive sulfhydryl groups in cellular proteins. The estrogen receptor (ER) contains cysteines in zinc (Zn) fingers that are susceptible to oxidation and internal disulfide formation, which can prevent DNA binding. We examined ER binding to its DNA response element …

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A Physiochemically Constrained Seawater Culturing System For Production Of Benthic Foraminifera, Christopher J. Hintz, G. Thomas Chandler, Jay M. Bernhardt, Daniel C. Mccorkle, Suzanne M. Havach, Jessica K. Blanks, Timothy J. Shaw Jun 2004

A Physiochemically Constrained Seawater Culturing System For Production Of Benthic Foraminifera, Christopher J. Hintz, G. Thomas Chandler, Jay M. Bernhardt, Daniel C. Mccorkle, Suzanne M. Havach, Jessica K. Blanks, Timothy J. Shaw

Faculty Publications

No abstract provided.

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Individual Cell Growth Rates Of Marine Bacteria, Measured By Bromodeoxyuridine Incorporation, Koji Hamasaki, Richard A. Long, Farooq Azam May 2004

Individual Cell Growth Rates Of Marine Bacteria, Measured By Bromodeoxyuridine Incorporation, Koji Hamasaki, Richard A. Long, Farooq Azam

Faculty Publications

We tested the application of 5-bromo-2’-deoxyuridine (BrdU), a thymidine analogue that becomes incorporated into DNA during growth, to measure growth rates of individual marine bacteria cells. Immunocytochemical detection of BrdU incorporation into bacterial DNA has the potential for single-cell-based growth measurement. Optimized procedure for immunocytochemistry was applicable to 14 marine heterotrophic bacterial isolates belonging to g-proteobacteria, α-proteobacteria, Cytophaga-Flavobacterium-Bacteroides (CFB) group and Gram-positive bacteria. The relationship between cell-specific fluorescence intensity and specific growth rate was linearly correlated among CFB group isolates, which indicated a potential of the method for quantitative measurement. Analysis of the detection limit indicated …

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From Colorblindness To Intercultural Sensitivity: Infusing Diversity Training In Pete Programs, Joe W. Burden Jr., Samuel R. Hodge, Camille P. O'Bryant, Louis Harrison Jr. May 2004

From Colorblindness To Intercultural Sensitivity: Infusing Diversity Training In Pete Programs, Joe W. Burden Jr., Samuel R. Hodge, Camille P. O'Bryant, Louis Harrison Jr.

Kinesiology and Public Health

In this paper, we advocate infusing diversity training across physical education teacher education (PETE) programs and curricula (DeSensi, 1995). Specifically, we call for PETE programs to provide curriculum content and professional socialization experiences that enhance intercultural sensitivity to better prepare novice teachers for working effectively with students of various cultures and ethnicities (DeSensi, 1995; Hodge, 2003). We discuss (a) changing demographics in society and schools with implications for preparing teachers, (b) moving from ethnocentricism to ethnorelativism of intercultural sensitivity, (c) implementing NCATE diversity initiatives, (d) infusing diversity training in PETE programs, and (e) understanding physical activity and sport participation patterns …

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Site And Mechanism Of Leptin's Antisteatotic And Insulin Sensitizing Actions In Lipodystrophic Liver, Esra Asilmaz Jan 2004

Site And Mechanism Of Leptin's Antisteatotic And Insulin Sensitizing Actions In Lipodystrophic Liver, Esra Asilmaz

Student Theses and Dissertations

An adipocyte hormone which functions as the afferent signal in a negative feedback loop regulating energy balance, has profound effects on glucose and lipid metabolisms. Lipodystrophic humans and mice have a complete or partial absence of adipose tissue and a secondary reduction of leptin. This is associated with insulin resistance and hepatic steatosis, which are corrected by leptin administration. This thesis aimed to define the site of leptin action and understand the molecular basis of leptin's insulin sensitizing and antisteatotic effects in lipodystrophy. To determine the site of leptin action, we treated lipodystrophic aP2-nSREBP-1c mice with a low dose of …

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Culture-Independent Analysis Of Fecal Enterobacteria In Environmental Samples By Single-Cell Mrna Profiling, Han Chen, Gomathinayagam Ponniah, Nancy Salonen, Paul H. Blum Jan 2004

Culture-Independent Analysis Of Fecal Enterobacteria In Environmental Samples By Single-Cell Mrna Profiling, Han Chen, Gomathinayagam Ponniah, Nancy Salonen, Paul H. Blum

School of Biological Sciences: Faculty Publications

A culture-independent method called mRNA profiling has been developed for the analysis of fecal enterobacteria and their physiological status in environmental samples. This taxon-specific approach determines the single-cell content of selected gene transcripts whose abundance is either directly or inversely proportional to growth state. Fluorescence in situ hybridization using fluorochrome-labeled oligonucleotide probes was used to measure the cellular concentration of fis and dps mRNA. Relative levels of these transcripts provided a measure of cell growth state and the ability to enumerate fecal enterobacterial cell number. Orthologs were cloned by inverse PCR from several major enterobacterial genera, and probes specific for …

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Inhibition Of Severe Acute Respiratory Syndrome-Associated Coronavirus (Sarscov) By Calpain Inhibitors And Beta-D-N4-Hydroxycytidine, Dale L. Barnard, V. D. Hubbard, J. Burton, Donald F. Smee, John D. Morrey, M. Otto, R. W. Sidwell Jan 2004

Inhibition Of Severe Acute Respiratory Syndrome-Associated Coronavirus (Sarscov) By Calpain Inhibitors And Beta-D-N4-Hydroxycytidine, Dale L. Barnard, V. D. Hubbard, J. Burton, Donald F. Smee, John D. Morrey, M. Otto, R. W. Sidwell

Animal, Dairy, and Veterinary Science Faculty Publications

We evaluated two types of compounds for efficacy in inhibiting SARSCoV replication in vitro: calpain inhibitors (a class of cellular cysteine proteinases) and a number of nucleoside analogues. Cytopathic effect reduction assays visually determined with spectrophotometric verification by neutral red (NR) uptake assay were used to evaluate cytotoxicity and antiviral potency of the compounds. Significantly inhibitory compounds were then evaluated in virus yield reduction assays. Two calpain inhibitors, Val-Leu-CHO (calpain inhibitor VI) and Z-Val-Phe-Ala-CHO (calpain inhibitor III), were the most potent inhibitors of SARSCoV. By virus yield reduction assay, calpain inhibitor VI had a 90% effective concentration (EC90) of 3 …

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Dinitrogen Fixation And Release Of Ammonium And Dissolved Organic Nitrogen By Trichodesmium Ims101, Margaret R. Mulholland, Deborah A. Bronk, Douglas G. Capone Jan 2004

Dinitrogen Fixation And Release Of Ammonium And Dissolved Organic Nitrogen By Trichodesmium Ims101, Margaret R. Mulholland, Deborah A. Bronk, Douglas G. Capone

OES Faculty Publications

Two methods used to measure dinitrogen (N2) fixation (acetylene reduction and 15N2 uptake) often result in different N2 fixation rates. Part of the discrepancy may arise from the observation that Trichodesmium can release a fraction of their recently fixed N2 as dissolved organic nitrogen (DON) and/or ammonium (NH4 +). To resolve outstanding issues regarding N2 fixation and the production of dissolved combined nitrogen (N) by Trichodesmium, we conducted a comprehensive analysis of N2 fixation and the production of DON and NH4+ in cultures of Trichodesmium IMS101. We performed …

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Interactive Regulation Of Dissolved Copper Toxicity By An Estuarine Microbial Community, Christina L. Dryden, Andrew S. Gordon, John R. Donat Jan 2004

Interactive Regulation Of Dissolved Copper Toxicity By An Estuarine Microbial Community, Christina L. Dryden, Andrew S. Gordon, John R. Donat

OES Faculty Publications

Cultured marine microorganisms under copper stress produce extracellular compounds having a high affinity for copper (copper-complexing ligands). These ligands are similar in binding strength to those found in natural waters, but few studies have examined the relationship between copper, copper-complexing ligand concentrations, and natural microbial populations. A series of in situ experiments in the Elizabeth River, Virginia, revealed that an intact estuarine microbial community responded to copper stress by production of extracellular, high-affinity copper-complexing ligands. The rate of ligand production was dependent on copper concentration and resulted in a reduction of the concentration of free cupric ions, Cu2+, …

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An Investigation Of Dissolved Organic Matter In A Shallow Coastal Bay Subject To Aureococcus Anophagefferens Blooms, Jean-Paul Simjouw Jan 2004

An Investigation Of Dissolved Organic Matter In A Shallow Coastal Bay Subject To Aureococcus Anophagefferens Blooms, Jean-Paul Simjouw

OES Theses and Dissertations

Aureococcus anophagefferens, the pelagophyte responsible for brown tide blooms, was identified in Chincoteague Bay in 1997 and has “bloomed” there since at least 1998. Aureococcus anophagefferens is capable of using dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) substrates to support growth, and this utilization is hypothesized to give the organism a competitive advantage relative to other phytoplankton when inorganic nutrient concentrations are low or depleted. Because previous studies suggest dissolved organic matter (DOM) is important in initiating and sustaining brown tide blooms, a field study of the variations in DOC concentration and DOM composition was performed at …

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Temporal And Spatial Expression Of Tissue Inhibitors Of Metalloproteinases 1 And 2 (Timp-1 And -2) In The Bovine Corpus Luteum, Bo Zhang, Marsha A. Moses, Paul C.W. Tsang Nov 2003

Temporal And Spatial Expression Of Tissue Inhibitors Of Metalloproteinases 1 And 2 (Timp-1 And -2) In The Bovine Corpus Luteum, Bo Zhang, Marsha A. Moses, Paul C.W. Tsang

Molecular, Cellular & Biomedical Sciences

The matrix metalloproteinases (MMPs) and their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs), may mediate the dramatic structural and functional changes in the corpus luteum (CL) over the course of its life span. In addition to regulating MMP activity, TIMPs are also involved in a variety of cellular processes, including cell proliferation and steroidogenesis. In a series of initial studies, we determined that matrix metalloproteinase inhibitory activity was present in protein extracts from early (4 days old, estrus = day 0), mid (10–12 days old) and late (16 days old) CL (n = 3 for each stage). Reverse zymography revealed …

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Spring 2003, Nsu Oceanographic Center Apr 2003

Spring 2003, Nsu Oceanographic Center

Currents

No abstract provided.

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