Digital Commons Network^™

Teaching Beyond The Textbook, Jada Fritzemeier

Celebrating Scholarship and Creativity Day (2018-)

As part of the Community Engaged Learning component in our Community Psychology class, we worked closely with St. Joseph Catholic School and Saint John’s Preparatory School to support students’ learning and understanding of their education and its values. Both schools have shared values and work to promote the integration of faith and the Benedictine values into their daily lives. While there, we provided one-on-one support to students with various academic and well-being needs as well as classroom assistance. These include after-school tutoring, mindfulness walks with students, interactive activities to promote community, and helping teachers and students in the classroom. By …

Unveiling The Biochemical Pathway Responsible For Ankyloglossia (Tongue-Tie) By Tracking Enzymatic Activity Of Apoptosis Pathways, Becca Ringstad

Celebrating Scholarship and Creativity Day (2018-)

Ankyloglossia (Tongue-tie) is a condition that is caused by failed cellular degeneration leading to a short lingual frenulum. This short lingual frenulum tethers the tongue to the bottom palate of the mouth and leads to problems, including breastfeeding for infants, depriving them of important proteins that build the gut microbiome, strengthen the immune system, and fight off antigens. If left untreated, it leads to problems with speech articulation, airway development, and obstructive sleep apnea. Treatment involves a frenectomy, which is the release of the short lingual frenulum using surgical scissors or a laser. Ankyloglossia can occur with conditions such as …

Lipid Deposition In Beef Cattle, Kyra L. Elliott

Department of Animal Science: Dissertations, Theses, and Student Research

Both the amount and types of lipid deposition in beef cattle are key determinants of carcass value and meat quality. Amounts of intramuscular, subcutaneous, and visceral fat influence carcass grading outcomes, palatability, and composition traits that are important to beef producers as well as consumers. The objectives of this thesis were to: 1) compare beef grading camera estimates of intramuscular fat to amounts of chemically extractable lipid, 2) characterize the lipidomes of beef intramuscular fat, subcutaneous fat, and lean muscle using shotgun lipidomics, and 3) identify and characterize a novel genetic variant in cattle that may have important implications for …

Characterization Of Activity Changes In Gilts During Estrus Using The NuTrack Livestock Monitoring System, Emily P. Aquino

Department of Animal Science: Dissertations, Theses, and Student Research

Early and accurate detection of estrus in replacement gilts is essential for reproductive success and to ensure efficient production. The objective of this study was to evaluate activity changes occurring relative to first estrus of gilts and subsequent cycles. At ~ 110 days of age, 112 gilts were randomly assigned to 14 pens and placed under continuous video monitoring until the end of the trial at ~ 200 days of age. Daily boar exposure (~ 10 minutes per day) began at ~ 122 days of age. During this period, recorded observations included vulva scores (based on swelling and redness), mounting …

Advancing Targeted Food Allergen Detection: Extraction Optimization And Sample Preparation Method Comparison Using Lc-Ms/Ms, Zhenhao Wang

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Accurate quantification of food allergens in processed foods by liquid chromatography-tandem mass spectrometry (LC-MS/MS) requires effective protein extraction and sample cleanup. However, no single sample preparation workflow has shown consistent performance across diverse food matrices. This study evaluated extraction buffer formulations and post-extraction cleanup strategies for quantification of milk, soy, and peanut allergens in multiple processed food matrices.

Six extraction buffers with varying combinations of SDS, urea, and thiourea alongside a common Tris-HCl/DTT/PVPP base were evaluated across five food matrices. No significant differences in extraction efficiency were observed among buffer formulations for nonfat dry milk (NFDM), non-roasted soy flour, peanut …

Cell-Type Specific Control Of Visual Fixation In Drosophila Melanogaster, Meagan Leung

Biological Sciences

Navigation in complex environments requires precise visual processing. To move effectively through their surroundings, animals must orient toward and maintain fixation on objects of interest to preserve a stable heading. However, the neural mechanisms underlying this behavior remain poorly understood. We use Drosophila melanogaster as our model organism because its visual behavior can be precisely measured and the availability of genetic tools which enables us to perturb the underlying neural circuits. Using a closed-loop virtual reality environment combined with locomotion tracking, we developed a stimulus protocol that reliably elicits fixation responses. To determine whether specific visual neurons drive fixation responses, …

Population Structure And Connectivity Of Atlantic Nurse Sharks (Ginglymostoma Cirratum) In The Bahamas And Outlying Areas, Baylie A. Fadool

School of Natural Resources: Dissertations, Theses, and Student Research

Understanding animal ecosystem dynamics and behavior at the individual and population levels is essential for identifying life-history patterns and important areas for management and conservation. Marine fish species often face minimal geographic barriers to movement, but environmental (temperature, salinity, current) or intrinsic barriers (philopatry, site fidelity) can dictate gene flow. Atlantic nurse sharks (Ginglymostoma cirratum) are an International Union for Conservation of Nature (IUCN) listed ‘vulnerable’ species and present a unique opportunity to investigate patterns of population structure and connectivity in large-bodied sharks. They are benthic and display reproductive philopatry, with recent work suggesting they have the capacity …

Exploring Student Sensemaking During A Collaborative Biology Lab, Rebecca Kollipara

School of Natural Resources: Dissertations, Theses, and Student Research

Collaborative learning environments offer valuable opportunities for students to construct scientific understanding through dialogue, yet the conditions that support sustained conceptual reasoning in group settings remain incompletely understood. While prior research has identified discourse moves associated with productive collaboration, less attention has been paid to how those moves function together across turns and conceptual boundaries, or how individual student characteristics shape their emergence. This thesis examines collaborative sensemaking in an undergraduate General Biology II course, drawing on two complementary analyses of student discourse during a bryophyte life-cycle modeling activity.

Chapter 1 investigates how student groups participated in sensemaking and how …

Gaining Mussel: Evaluating Reintroduction Success Of Native Freshwater Mussels In Nebraska And Eastern Wyoming, Travis A. Moore

School of Natural Resources: Dissertations, Theses, and Student Research

Freshwater mussels are one of the most imperiled groups of organisms in North America, with over two-thirds of species considered threatened, endangered, or of special concern. These long-lived bivalves are essential to stream ecosystems, acting as filter feeders that improve water quality and as indicators of aquatic health. In Nebraska and Wyoming, native mussel populations have declined sharply due to habitat modification, pollution, and reduced connectivity. Two species of particular concern are the Plain Pocketbook Lampsilis cardium, listed as Tier I at-risk in Nebraska, and the Fatmucket Lampsilis siliquoidea, a Tier II at-risk species. In response, state agencies …

The Impact Of Glucose Exposure On Organic Cation Transporters (Oct) Function In A Corneal Epithelial Cell Line, David A. Garza

Theses & Dissertations

Organic cation transporters (OCT) facilitate the movement of neurochemicals and medications, contribute to the removal of cellular waste, and facilitate neuronal communication. Three subtypes of these high-capacity, low-affinity transporters (OCT1, OCT2, and OCT3) are differently expressed in peripheral tissues such as the liver, kidneys, as well as the central nervous system. OCTs are also expressed in ocular tissues. Chronic hyperglycemia is a complication commonly associated with diabetes that can lead to ocular diseases such as diabetic keratopathy, which affects the cornea. In this project, we expose immortalized human corneal epithelial cells (HCE-S) to various glucose concentrations to study their impact …

Validation Of A Real-Time Pcr Laboratory Developed Test For The Detection Of Pneumocystis Jirovecii In Bronchoalveolar Lavage Samples, Caitlyn Moon

Honors Scholar Theses

Pneumocystis jirovecii is an opportunistic, host-obligate fungal pathogen responsible for causing P. jirovecii pneumonia (PJP) in humans. PJP is treated with trimethoprim-sulfamethoxazole (TMP-SMX), a strong broad-spectrum antibiotic that carries risk of renal and hepatotoxicity. Therefore, rapid diagnosis or exclusion of PJP is crucial to ensure PJP-positive patients receive effective treatment and PJP-negative patients avoid unnecessary antibiotics. Unlike most microorganisms, P. jirovecii cannot be cultured, presenting complications for the typical methods of diagnosing fungal pathogens. Historically, the gold standard for diagnosis was direct microscopic examination with stains such as methenamine silver and calcofluor white, but this diagnostic technique requires highly skilled …

The Development Of A Crispr-Based Calibrated Functional Assay For Classifying Pathogenicity Of Msh2 And Msh6 Variants In Lynch Syndrome, Olivia N. Amodeo

Honors Scholar Theses

Lynch syndrome is a hereditary disease caused by the inheritance of a mismatch repair gene variant. Individuals with this condition are predisposed to cancer development, most commonly colorectal cancer. Current guidelines for variant classification are based on numerous evidence categories, including functional evidence. However, many novel clinical variants are not well characterized, and evidence is difficult to obtain if functional assays are not calibrated.

To address this, our lab created a calibrated functional assay that calculates an odds of pathogenicity score for MSH2 and MSH6 gene variants that can be used as evidence for classifying variants of uncertain significance. This …

Phenotypic Contribution And Regulation Of Vibrio Parahaemolyticus Cold-Shock Protein Homologue Vp1889, Oleg Borisovich Ermolinsky

Theses and Dissertations

Vibrio parahaemolyticus is the primary causative agent of a form of gastroenteritis called vibriosis commonly acquired from ingestion of raw and undercooked seafood. The primary mitigation of seafood-borne illnesses is immediate storage on ice following catch, which inhibits bacterial metabolism and replication. Mesophilic bacteria such as V. parahaemolyticus induce expression of cold-shock proteins in a process named the cold shock response, which facilitates survival at low temperatures. V. parahaemolyticus bears 3 homologues of the major Escherichia coli cold-shock protein CspA, an RNA chaperone. Induction of E. coli CspA at low temperatures depends on autogenous modulation by its mRNA structure, which …

Ethanol State-Dependent Learning In The Honey Bee (Apis Mellifera), Ethan Neil Govea

Theses and Dissertations

Ethanol disrupts neural circuits involved in addiction and withdrawal, yet the molecular mechanisms underlying these effects remain poorly understood. One possibility is that ethanol alters an animal’s perceptual or internal state, causing a stimulus learned while intoxicated to be processed differently when the animal is sober. This study examined how ethanol exposure affects odor learning, recall, and discrimination in honey bees, a useful model because of their robust olfactory learning and experimentally accessible neural systems. Bees were differentially conditioned with appetitive reward (sucrose) during hexanol exposure and aversive stimulus (quinine) during decanol exposure. To assess olfactory processing and state dependency, …

Developing A Qpcr Method For Evaluating Parasitoid Wasps As A Potential Means Of Control Of The Southern Cattle Fever Tick Rhipicephalus (Boophilus) Microplus In South Texas, Abbey Abalos

Theses and Dissertations

Ixodiphagus spp. wasps are natural enemies of ticks that have been reported to cause mortality in multiple tick species. Parasitoid wasps may represent a potential biocontrol alternative to traditional chemical tick control methods, which are challenged by non-target toxicity and the development of pesticide resistance in species of concern such as Rhipicephalus microplus, a pest of livestock that causes billions of dollars in loss to the livestock industry worldwide. Rhipicephalus sanguineus s.l. is a congeneric species of R. microplus, and parasitism of R. sanguineus by Ixodiphagus sp. in areas of co-occurrence has been observed. It is unknown if R. microplus …

Investigating The Ability Of Foxo1 To Maintain Stem Cells, Stella Aime Rios

Theses and Dissertations

Forkhead box O (Foxo) transcription factors are key regulators of stem cell identity and tumor aggressiveness. Prior work from our group and others demonstrates that Foxo factors maintain stem-like states across diverse biological systems; however, the mechanisms by which Foxo1 suppresses differentiation remain poorly defined.

Using C2C12 myoblasts as a model, we investigated whether Foxo1 preserves stem-like programs through regulation of the nuclear pore protein Nup210. RNA interference was used to generate Foxo1 knockdown cells, followed by qRT-PCR, RNA-sequencing, and fluorescence microscopy. Transcriptomic analysis revealed widespread gene expression changes involving metabolic, mitochondrial, and transcriptional pathways. Notably, Nup210 expression was significantly …

Effects Of Weight Loss Medications In Combination With Psychotropic Medications, Emily A. Hoffman

Electronic Theses, Projects, and Dissertations

Background and aims: The obesity epidemic has been well-documented and weight loss medications are becoming increasingly popular. Patients with psychiatric conditions often suffer metabolic side effects from psychotropic medications and are often prescribed weight loss medications to mitigate these side effects. When treating patients on these medications concurrently, understanding their potential side effects is essential for developing an effective care plan. Registered dietitian nutritionists (RDNs), pharmacists, and mental health professionals working in mental health facilities should be aware of these potential side effects to better care for their patients.

Methods: After completing a thorough literature review, a handbook …

Can Carpobrotus Edulis Cause Significant Changes To The Soil Microbiome?, Samantha J. Cowan

Electronic Theses, Projects, and Dissertations

Invasive species cause significant damage to ecosystem biodiversity and can cost billions of dollars to restore native species (Van der Wal et al, 2008; Diagne et al., 2021).  In certain instances, even if the invasive species are removed, they can leave legacy effects on the environment that make restoration more challenging (Conser and Connor, 2008; Elgersma et al., 2011). Carpobrotus edulis is an invasive species of plant that is known to cause changes to its environment such as affecting the soil nitrogen cycle and the pH, potentially leading to persistent changes in the soil microbiome and causing native species to …

Mining The Usda Snap Bean Association Panel For Resilience: Genetic Dissection Of Heat And Disease Tolerance In Snap Bean, Atiya F. Khan

All Theses

Snap bean (Phaseolus vulgaris L.) is an economically and nutritionally important vegetable crop whose production is increasingly threatened by rising temperatures and emerging bacterial diseases, particularly in the southeastern United States. Heat stress during reproductive development can significantly reduce yield, while newly emerging pathogens such as Pseudomonas capsici present additional risks to crop productivity. This study leveraged the USDA Snap Bean Association Panel (SnAP) to investigate the genetic basis of heat tolerance and disease resistance and to identify germplasm and genomic regions useful for breeding resilient cultivars.

To evaluate heat tolerance, a subset of the SnAP panel was …

Micro-Solar Kiln Design: A Cost-Effective Approach For Small Landowners To Produce Higher Quality Lumber, Bart Swecker

All Theses

Small-scale and hobby sawmill operators increasingly seek low-cost methods for drying lumber to moisture contents suitable for interior use, yet conventional kiln systems are often impractical due to capital cost, power requirements, and operational complexity. While air drying remains common at small scales, many operators require a controlled finishing step to improve dimensional stability and product value. Solar kilns offer an intermediate solution between air drying and conventional kilns; however, most published designs and performance data focus on systems with capacities far larger than those typically used by small-scale operators. Consequently, limited guidance exists for micro-scale solar kilns, particularly under …

Biology, Ecology And Control Of Eye Gnats In An Open Pine Ecosystem, Leela Hospach

All Theses

Eye gnats of the genus Liohippelates are bothersome flies of the family Chloropidae. They breed in moist, disturbed, sandy soils that contain organic matter, and have been implicated as vectors of conjunctivitis, bovine mastitis, and yaws. We conducted research into some basic biological and ecological characteristics. Using wild-caught gnats in a temperature-controlled incubator, we found that their activity peaks between 27°C and 41°C. Trapping at different heights above the forest floor in an open-pine savannah, we captured most gnats at 7.5 m off the ground. This may have to do with the attractiveness of the rotten egg lures we used, …

Characterizing The Molecular Response Of Embryonic Zebra Finch To Acute Sound Playback, Saidat O. Adeniran-Obey Miss

All Theses

Auditory stimuli are important cues that influence developmental plasticity in animals and are important for development and adaptation to an ever-changing environment. In the zebra finch (Taeniopygia guttata), there is evidence of adaptive developmental reprogramming in response to a prenatal ‘heat call’. Embryos exposed to this heat call exhibit changes in growth rate post-hatch, increased heat resistance, and enhanced reproductive success at maturity. However, it has long been assumed that embryonic zebra finches cannot respond to sound due to the immaturity of their auditory system. These findings raise the critical question of whether late-stage zebra finch embryos can, …

Comparative Biomechanics: Blattodea Antennal Morphology And Physiology, Lucas W. Marsh

All Theses

Equipped with muscle-free antennae, many insects can twist and bend their antennae on demand. We hypothesized that hemolymph circulating through the antennae provides pressure that facilitates twisting and bending and provides a feedback control channel for tactile sensing in insects. To test this hypothesis, we conducted a thorough morphological and physiological analysis of antennae in three Clades of Blattodea. We studied morphology by evaluating the distinguishable features of the body and antenna, as well as the internal structure of the antennal lumen. Then, in a series of experiments on live cockroaches, we measured blood pressure along the antenna. This was …

Ontogeny And The Effects Of Perinatal Stress In The First Immature Skeleton Of Teleoceras Aepysoma (Perissodactyla, Rhinocerotidae), Joe Gajewski

Electronic Theses and Dissertations

Recovery of an immature fossil rhinoceros (Teleoceras aepysoma) skeleton (ETMNH 32999) from the Gray Fossil Site in Tennessee offers the first insights into the ontogeny of the species. ETMNH 32999 is interpreted to be a subadult male in an age class equivalent to ~17-20% of potential lifespan. Comparative analyses support the hypothesis that subadults of related taxa are typically ~91% of average adult size in most dimensions. However, certain cranial dimensions are substantially lower than expected in ETMNH 32999, averaging only ~74% of the average conspecific adult. Such departure from typical growth rates is suggested to be attributable …

Changing Dynamics Of Microbial Communities At A Hydrocarbon Contaminated Site And Methanogenic Interactions With Iron Minerals, Jakob Sell

Masters Theses

Microbial life is not only ubiquitous but also plays a critical role in the geochemical makeup of our planet. While researchers have long studied easily culturable aerobic microbes, the influence of anaerobic life on soils and mineral cycling is less understood. Notably, knowledge about interactions between anaerobic microbes and inorganic elements such as iron has progressed with the discovery that magnetite influences how anaerobic archaea carry out methanogenesis. To better understand anaerobic bacterial metabolisms and how they interact with minerals and subsurface hydrocarbons, we extracted 16S rRNA genes from two adjacent subsurface petroleum plumes, sequenced the DNA, and analyzed it …

Redeveloping Mississippi: A Case Study On Brownfields In Central & Southern Mississippi, Sahrah R. Yeroozedek

Master's Theses

Brownfields, or properties that are or possibly are contaminated with dangerous materials, represent a common obstacle for communities in Mississippi. The real or potential presence of contaminants at these sites often deters investors due to costly cleanup requirements (EPA, n.d. -b). The Environmental Protection Agency (EPA), through its Brownfield Revitalization Program, implemented guidelines for covering the costs and labor associated with the remediation of these properties. The EPA is not, however, responsible for redevelopment that supports long-term environmental and economic sustainability. Although the EPA maintains inventories of all Brownfields, there is also no comprehensive database of Brownfields and their current …

The Impact Of High-Cadence Cycling On Overground Running Performance In Recreational Runners, Matthew Ott

Master's Theses

Cycling is a commonly used exercise because it provides a controllable cardiovascular and musculoskeletal demand while imposing relatively low impact on the rider. High-cadence cycling (HCC) has been shown to influence subsequent running performance and gait performance by increasing cadence and reducing work rate when cycling prior to running. However, most of this work has focused on triathletes and older adults. Therefore, the purpose of this study was to examine the acute effects of HCC on subsequent 800 m running performance and spatiotemporal parameters in adult recreational runners. Thirty recreational runners completed two testing sessions. On Day 1, participants performed …

Degradation And Metabolic Analysis Of Synthesized Quorum-Sensing Signals By The Model Eukaryote Chlamydomonas Reinhardtii, Adam Josef Bach

Theses and Dissertations

Bacterial organisms can “talk” with one another on a colony-wide scale using a communication system known as quorum-sensing (QS) via chemical signals. These signals are produced at a basal rate throughout the life of the bacteria and are classified as autoinducers, which diffuse across the cell membrane. Once the critical concentration is reached, they begin to diffuse back into the cell and initiate a phenotypic change. As these signals exist outside the membrane of their producer, they are also accessible to other organisms. It has already been noted that some plant species uptake these compounds through their roots and break …

A Tractable Nematode Model For The Emerging Fungal Pathogen, Candida Auris, Melissa Martinez

Dissertations and Theses (Open Access)

Candida auris is an emerging multidrug-resistant opportunistic fungal pathogen. It

poses a significant threat to immunocompromised patients and those with prolonged stays in

healthcare facilities. C. auris spreads rapidly in health care facilities through enhanced

colonization of skin and abiotic surfaces. The genetic factors contributing to this ability and to

the virulence, drug resistance, and stress-tolerant nature of C. auris are largely unknown.

Current animal models of C. auris infections are not well standardized and report conflicting

results. Additional animal models of virulence are needed, especially those amenable to high-

throughput analysis. The nematode Caenorhabditis elegans has been validated as …

Pattern Dynamics And Stochasticity Of Brain Rhythms And Spike Trains In A Tauopathy Mouse Model Of Alzheimer’S Disease, Clarissa M. Hoffman

Dissertations and Theses (Open Access)

Systems neuroscience posits that every aspect of perceived physical reality, every aspect of animal and human behavior, and every cognitive phenomenon emerges from patterns of neuronal activity. While most researchers embrace this idea, there are major difficulties in describing and analyzing these complex neuronal dynamics—spike flows produced by cells ensembles, synchronized extracellular field oscillations, and other patterns—which limits our understanding of how the activity of individual neurons and the whole-animal cognition and behavior might be connected. In particular, we lack the approaches and even the semantics for connecting the individual cell outputs and the integrated results of their activity. Current …