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Heat Shock Factor 1 Signaling: A Novel Pathway Implicated In Rett Syndrome Pathophysiology, Sonia Gonzalez

Dissertations

Rett syndrome (RTT) is a severe neurodevelopmental disorder that is associated with loss-of-function mutations in the methyl-CPG binding protein 2 (MECP2) gene. There are limited therapeutic options to address the wide range of social, cognitive, motor, and respiratory symptoms that patients experience throughout their lifetime. To identify novel therapeutic targets, we leveraged a rare form of RTT known as MeCP2-mutation-negative or atypical RTT. Traditionally, RTT is first diagnosed clinically and then confirmed through the identification of mutations in the MECP2 gene. However, ~5% of RTT patients are found to be MeCP2-mutation-negative (atypical), despite sharing overlapping symptomology with typical RTT. We …

Silent Invaders: Stopping The Spread Of The Spotted Lanternfly, Sloane Z. Hall

Masters Theses

The Spotted Lanternfly, an invasive insect originating from China, has rapidly spread across the United States, harming agriculture, forestry, and surrounding ecosystems. It’s destructive feeding behavior severely impacts grapevines, fruit trees, and hardwoods, which directly affects wine production, farm communities, and local environments. The aim of this research was to investigate the biological, ecological, and economic stakes of the infestation, as well as the methods currently used to monitor and control its spread. Specifically, it examines how communication frameworks, design practices, and extension approaches influence producers’ adoption of recommended Integrated Pest Management (IPM) protocols. Through the research, a literature review, …

Chronotropic Response To Intratracheal Versus Intravenous Atropine In Anesthetized Adult Patients: A Randomized Controlled Trial, Ammar H. Mahdi, Majid Alhamaidah, Myasar J. Mohammed, Hussein Ali Hussein, Hussein Alkhfaji

AUIQ Complementary Biological System

Atropine is a commonly used anticholinergic agent in anesthetic practice, administered primarily to counteract bradycardia. Rapid treatment of intraoperative bradycardia is clinically critical, as delays may lead to hemodynamic compromise. This study aimed to compare the onset, magnitude, and duration of the chronotropic response to atropine administered via the intravenous (IV) versus intratracheal (IT) route in anesthetized adult patients. A prospective, single-blind, randomized controlled study was conducted on 60 adult patients classified as American Society of Anesthesiologists (ASA) physical status I-II, scheduled for elective peripheral surgery under general anesthesia. Patients were randomly allocated into three equal groups (n = 20 …

Patterns And Drivers Of Cladocera Community Structure In The Black Sea, Elena Bisinicu, Luminita Lazar

Turkish Journal of Zoology

Cladoceran community dynamics were examined in the Romanian sector of the Black Sea during the warm seasons of 2013– 2020, using Marine Reporting  Units (MRUs) to evaluate spatial heterogeneity across transitional, coastal, and  marine waters. Analyses of density, biomass, mean individual biomass (MIB), and  species composition revealed interannual fluctuations superimposed on strong  nearshore–offshore gradients, with higher values in transitional and coastal MRUs.  The assemblage was consistently dominated by Pleopis polyphemoides and Penilia  avirostris, which together accounted for most of the total abundance and biomass.  Multivariate analysis identified significant differences in taxonomic composition  across MRUs and years, while correlation analyses highlighted …

Endemism Patterns Of Leaf Beetles (Coleoptera: Chrysomelidae) In Türkiye, İsmai̇l Şen, Ali̇ Gök

Turkish Journal of Zoology

In this study, the distribution of endemic leaf beetles in Türkiye was analyzed  holistically at the provincial scale using species richness (ENR), corrected weighted  endemism (CWE), and range-restricted endemism (RRE) indices. A total of 95  endemic taxa were found in Türkiye and these taxa exhibited significant spatial  heterogeneity in terms of richness and extent of distribution. The Western and  Central Taurus Mountains and the Eastern Black Sea Region were found to be the  main centers of endemism, with high densities of narrowly distributed and  geographically restricted species. These patterns clearly demonstrate that key  biogeographic factors, such as steep elevation gradients, …

The Heterogeneous Response Of Astrocytes In Different Brain Regions Under Metabolic Stress, Pawan Kumar Thapaliya

USF Tampa Graduate Theses and Dissertations

Astrocytes play a central role in maintaining ionic homeostasis, neurotransmitter clearance, and brain en-ergy metabolism. Their excitability, reflected in activity-evoked intracellular sodium (Na+, [Na+]i) and calcium (Ca2+,[Ca+]i) dynamics, is increasingly recognized as a critical determinant of neuronal function and vulnerability, particularly under metabolic stress. Despite their importance, the mechanisms underlying astrocytic ionic signaling and its regional heterogeneity remain incompletely understood. In this thesis, I developed detailed computational and biophysical models to investigate astrocytic Na+ and Ca2+ dynamics, their bioenergetic consequences, and the roles of key ion transporters under physiological and metabolically stressed conditions, validating the models against experi- mental data. …

Preserving The Harvest: How To Preserve Chokecherries, Callahan K. Adams, Andrea Schmutz

All Current Publications

A treasured wild fruit in Utah and across the Intermountain West, chokecherries offer both cultural heritage and culinary versatility. With proper handling and research-tested preservation methods, you can safely transform them into delicious jellies, syrups, and more. Whether using a steam juicer or stovetop method, following tested recipes and adjusting for elevation ensures both safety and quality. This fact sheet provides information and recipes to safely preserve chokecherries.

In Vitro Reversal Of Abc Transporter Mediated Multidrug Resistance In Human Ovarian And Non-Small Cell Lung Cancer Models, Alison K. Kellom, Pia D. Vogel

Biological Sciences Theses and Dissertations

One of the major causes of treatment failure in aggressive cancers is multidrug resistance (MDR), which is linked to the overexpression of membrane efflux proteins that export chemotherapeutics from cancer cells. This mechanism prevents chemotherapeutic drugs from reaching cytotoxic concentrations intracellularly, allowing the cancer to survive. Two primary mediators of this mechanism are P-glycoprotein (P-gp) and Breast Cancer Resistance Protein (BCRP). P-gp and BCRP are transmembrane ATP-binding cassette (ABC) transporters that are frequently overexpressed in MDR cancers. They utilize the binding and hydrolysis of ATP to transport a diverse range of amphipathic molecules of varying size (Schinkel and Jonker, 2003), …

The Concept 'Pollination' And Bee Pan-Trap Color And Uv Reflectance Determine The Taxonomic Compositions Of Bee Taxa Captures Across An Elevation Gradient And Seasons In The Jemez Mountains Of New Mexico, Esther A. Burton, Kelly B. Miller, Paul J. Watson, David C. Witherington, Steven Poe, David Lightfoot

Biology ETDs

This thesis examines the pollinator concept and tests how sampling methods shape bee communities. Chapter 1 provides a conceptual and philosophical analysis, arguing that pollination is a process and that pollinators are context-dependent functional roles rather than fixed taxonomic categories. It traces the concept’s development from natural history to ecological theory, introduces a dual-goal model separating plant reproduction from visitor foraging, and proposes a classification framework based on evidentiary certainty and pollination potential to resolve ambiguity in pollination research.

Chapter 2 applies these ideas empirically by evaluating how pan-trap color and ultraviolet (UV) reflectance influence bee assemblages across elevation and …

Investigating Changes In The Stable Isotopic Composition Of Juniperus Osteosperma Over 34,000 Years Of Climatic Shifts, Lisa S. Garcia

Biology ETDs

Isotopic compositions of plants from the past provides empirical evidence for paleoclimatic shifts and plants physiologically response. This study used Neotoma paleomiddens (fossilized debris piles) collected from Death Valley National Park, which contained preserved abundant plant materials. These middens range from modern to >35,000 years and capture different climatic events in Earth history. Employing Juniperus osteosperma (Utah Juniper) leaves found in middens, I investigated variation in carbon and nitrogen stable isotopic composition. To help interpret paleomidden results, we used modern field and herbaria samples of Juniperus from a spatial gradient stretching across their geographic range. Results showed δ ¹³C …

Rhythms Of Hippocampal Function And Memory After Moderate Prenatal Alcohol Exposure, Lilliana May Sanchez

Psychology ETDs

Fetal Alcohol Spectrum Disorders involve morphologic and neurobiological abnormalities in offspring subjected to prenatal alcohol exposure. Although not as well studied as high exposure, moderate prenatal alcohol exposure represents the most common and undetected form in humans. While learning and memory deficits have been well characterized at the behavioral level in humans with Fetal Alcohol Spectrum Disorders and in animal models of moderate prenatal alcohol exposure, few studies have determined the circuit or systems level mechanisms of the behavioral deficits. A complete understanding of the neural bases of memory deficits after moderate prenatal alcohol exposure is needed to ultimately identify …

Characterizing A Potential Cbp/P300 Histone Acetyltransferase Homolog In Tardigrade Germline Specification And Development, Brittney Michelle Hartshorn

Honors Projects

Germline specification is a fundamental process that ensures the proper development of germ cells. Germline specification occurs either through germ cell determinants maternally inherited through the mechanism of preformation or through induction, where cell interaction and epigenetic modifications direct specification. Determining how these mechanisms evolved and diverged remains an important question in developmental biology. Despite research on germline regulation in model organisms such as Caenorhabditis elegans or Drosophila melanogaster, such regulation remains poorly understood in many other animal groups. Tardigrades are an emerging model system for investigating developmental mechanisms due to their phylogenetic placement and experimentally tractable embryos. CBP/P300 is …

Computational Design Of Peptides And Proteins Through Machine Learning Approaches, Emily J. Hendrix

Chemistry and Chemical Biology ETDs

Advancements in machine learning have emerged as a pivotal tool in computational biochemistry, offering new advancements to address challenges in protein structure and function. However, current machine-learning approaches offer limited insight in understanding protein dynamics. The purpose of this work is to combine traditional physics-based computational tools, such as molecular dynamics and coarse-grained simulations, with recently developed AI-driven computational tools to bridge gaps and advance the understanding of proteins in both structural and dynamic aspects. I investigated several approaches such as (i) traditional physics-based methods to study protein conformation and ensembles; (ii) identifying a peptide inhibitor for the PICK1 PDZ …

Urobiome Composition Varies With Vesicoureteral Reflux Grade And History Of Febrile Urinary Tract Infection, Theresa C. Barrett, Kelvin Li, Adam Fitch, Asha Patel, Bhanu Sharma, Jacqueline Holden, Kermina Abdelsayed, Nina N. Kowalewski, Sridhar Narla, Daniel Bushnell, Barbara A. Methé, Catherine S. Forster

Department of Medicine Faculty Papers

Vesicoureteral reflux (VUR) is a risk factor for urinary tract infection (UTI). However, data describing the urobiome in children with VUR are limited. Here, we characterized the urobiome in children with and without VUR to better understand its potential role in VUR pathogenesis. Catheterized urine samples were obtained from children who presented for voiding cystourethrogram (VCUG) between 12/1/2020 and 10/3/2023. Children were excluded if they had any surgical procedures of the genitourinary tract in the 30 days prior to VCUG or were on treatment antibiotics at the time of VCUG. 16S rRNA gene sequencing and taxonomical classification identified the urobiome. …

Influence Of The Discipline Of Authentic Movement Practice On Interpersonal Relationships, Joy Ruben Welts

Educational Studies Dissertations

This mixed methods study explored how practicing the Discipline of Authentic Movement impacts relationships. This research was conducted to fill in the gaps around attunement and use of the Discipline of Authentic Movement as an intervention to support healthy relationships. Five self-selected adults met on Saturday afternoons for eight weeks to practice the Discipline of Authentic Movement. They completed a pre and post Revised Adult Attachment Scale and reflected weekly on their experience through journaling. Participants reported that it was the structure of the Discipline of Authentic Movement that allowed them to feel more comfortable being themselves with others. The …

Genomics And Bioinformatics Student Companion Workbook (First Edition), Na Xu, Tina Hu

Open Educational Resources

This Genomics and Bioinformatics Student Companion Workbook provides an integrated, hands-on introduction to the principles and applications of modern genomics and bioinformatics. Designed for undergraduate students, the workbook guides learners from foundational concepts of genome structure and sequencing technologies to advanced topics including variant analysis, comparative genomics, and multi-omics integration. Emphasizing the interplay between experimental biology and computational analysis, it introduces key analytical tools and biological databases that support genome interpretation and data-driven discovery.

Through a structured progression of chapters, students explore genome organization across diverse biological systems, from prokaryotes and viruses to complex eukaryotic genomes, and examine how genomic …

Patterns Of Molecular Evolution Driving Reproductive And Genomic Processes In Sauropsids, Taylor Green

Theses and Dissertations

Sexual reproduction is an important driver of genetic diversity and speciation through such forces as selection, mutation, and drift. Sauropsids are an appropriate clade to study shared molecular drivers of sexual and genomic evolution due to their genome architecture, sex determination mechanism (SDM) plasticity, and large syntenous genomic regions. Using comparative phylogenetic methods, I identify predictable patterns of selection in meiotic recombination genes and clade-specific drivers of recombination rate evolution. Then, leveraging whole genome sequencing, I demonstrate that American alligator genomes are characterized by an ancient loss of genetic diversity driven by drift and historic inbreeding. I also generate a …

The Extraction And Chemical Characterization Of The Avian Pigments Turacin And Turacoverdin, Sarah R. Bekkali

Honors Scholar Theses

Bird coloration is a trait that extends beyond mere aesthetics as it has an extensive range of biological significance. Plumage patterns and hues can influence camouflage, mate choice, social dominance, and physiological performance. Bird fitness, their ability to survive and reproduce, is greatly dependent on color. Melanins, carotenoids, and pterins are well-studied pigment systems that are commonly found across many avian species’. Alternatively, porphyrin-based pigments are rare and less-studied as they only found in turacos a sub-Saharan African bird belonging to the family Musophagidae. This thesis focuses on two pigments of interest: turacin, the deep crimson-red pigment found in …

Junctional Actin As A Mechanical Hub For Supracellular Actomyosin Force Transmission During Apical Constriction, Jasneet Brar

UNLV Theses, Dissertations, Professional Papers, and Capstones

Apical constriction is a conserved morphogenetic mechanism that drives epithelial folding during development. During ventral furrow formation in Drosophila melanogaster, contractile actomyosin networks generate forces that reduce apical cell area resulting in tissue invagination. For these cell-scale forces to produce coordinated tissue-scale changes, contractile networks must be mechanically integrated across neighboring cells. Although adherens junctions are known to mediate this coupling, it is unclear how junctions are integrated into cortical actin cytoskeleton, which directly determines cell, and consequently tissue, shape. This thesis tests the hypothesis that supracellular actomyosin organization during apical constriction depends on distinct adherens junction based mechanical couplings: …

A Tale Of Two Mammals: Tenrec Ecaudatus And Homo Sapiens, Debra Renee Kerr

UNLV Theses, Dissertations, Professional Papers, and Capstones

Most modern mammals maintain stable body temperatures (Tb) ~37ºC and high metabolic rates. Hibernators, like ground squirrels, are homeothermic during the active summer season, but are heterothermic during the hibernation season. During the hibernation season, ground squirrels follow a predictable pattern of torpor bouts interrupted by interbout arousals. Tenrecs are unusual because they are heterothermic and heterometabolic even during the active season. Previously, ground squirrel bile constituents were shown to be predictive of whether a squirrel is in the summer active or winter hibernation season. We found tenrec bile constituents were highly variable and not predictive of seasonal and metabolic …

Examining Demographic And Contextual Differences In Stress-Related Sport Interference Among U.S. Collegiate Sport Participants, Heather Tran

UNLV Theses, Dissertations, Professional Papers, and Capstones

Collegiate athletes experience a range of stressors that may interfere with sport performance; however less is known about how stress-related sport interference varies across demographic groups and contexts. Guided by the transactional model of stress and coping, this study examined differences in stress-related sport interference by gender, race/ethnicity, and year in school, as well as across situational contexts (training, competition, and life outside of sport). Data were drawn from a secondary dataset of 288 collegiate sport participants who completed the Sport Interference Checklist (SIC). Independent samples t-test and analyses of variance (ANOVA) were used to examined demographic differences, and a …

Contemporary U.S. Anthromes As Defined By Hanpp Regimes, Aishwarya Chandrasekaran, Kat F. Fowler, Christopher Lant

Environment and Society Faculty Publications

The concepts of anthromes and human appropriation of net primary production (HANPP) are both valuable in understanding our human-dominated planet, yet they have never been integrated theoretically or empirically. Here we utilize an extensive county-level dataset on HANPP and its product-level components to derive, through cluster analysis, ten contemporary US anthromes. From highest to lowest density of harvested HANPP, the anthromes are: rainfed corn–soy, dairy fodder, spring wheat–small grain, dryland winter wheat, subtropical soy–cotton, commercial timber, mixed hardwood and pasture, recovered eastern forest, prairie–sagebrush rangeland, and arid and alpine sparse grazing. Expanding to thirteen anthromes maintains these, while bifurcating the …

Accuracy Of Parameter Estimation For A Simple Gene Regulatory Network Model Is Sensitive To Network Motif, Number Of Parameters Estimated, And Magnitude And Direction Of Regulatory Relationships, Nikki C. Chun, Kam Dahlquist

Honors Thesis

A gene regulatory network (GRN) is a set of transcription factors that regulate the expression of genes encoding other transcription factors. The dynamics of a GRN explain how gene expression changes over time. GRNmap is a MATLAB software package that uses ordinary differential equations to model dynamics of small-scale GRNs. We used the program to estimate production rates, expression thresholds, and regulatory weights for each transcription factor in three related literature-derived GRNs based on yeast cold shock microarray data previously collected in the Dahlquist Lab. We noticed large differences in estimated weight values when 1-2% of the expression values were …

From Flight To Hatching: Adult Sexual Dimorphism, Life History, And Egg-Stage Ecology Of Pseudocotalpa Giulianii, Mandylene Mountain

UNLV Theses, Dissertations, Professional Papers, and Capstones

The Nevada endemic dune beetle Pseudocotalpa giulianii is currently known only from two dune systems in Amargosa Valley, Nevada, yet much of its life cycle occurs below the sand surface and remains poorly documented. This thesis addresses key gaps in adult-stage biology and egg-stage development that are relevant to understanding reproduction, vulnerability, and habitat requirements in this dunerestricted species.

To characterize adult sexual dimorphism and life history, I quantified morphological differences between males and females and measured sex-specific adult survival under laboratory conditions relative to the aboveground flight season. Males and females overlapped broadly in external body size, but tarsal …

Determining Agricultural Literacy Proficiency Of Extension Professionals And Primary Volunteers In Mississippi, Molly Campbell

Theses and Dissertations

The development of agricultural literacy is a lifelong process, moving from gaining agricultural knowledge to communicating with others to applying to societal issues, and extends beyond the formal classroom. The Mississippi State University Extension Service has the ability to reach various audiences through educational programming, making them a potential catalyst for change in increasing agricultural literacy across the state. The study aims to determine the agricultural literacy levels of Extension professionals and primary volunteers in Mississippi and the factors potentially influencing these levels. Twenty-nine Extension professionals and eleven primary volunteers completed a survey of demographic questions and the Judd-Murray Agricultural …

From Miocene Origins To Ecological Diversification: Unraveling Evolutionary Dynamics Of North American Asters (Symphyotrichum), Sushil Dahal

Theses and Dissertations

Symphyotrichum, a plant genus traditionally treated within the genus Aster L., comprises >100 species of annual or perennial plants in the Compositae family Asteraceae. One of the most diverse genera in the southeastern US flora, the genus extends across the Americas, the Caribbean as well as eastern Eurasia. Despite its presence across the major American biomes and with distinctive floristic characteristics, several question regarding Symphyotrichum remains obscure. This research project focuses on exploring diverse aspects of Symphyotrichum systematics, biogeography and evolution. I tested three main hypotheses: (1a) Symphyotrichum originated ancestrally across Mexico and eastern North America in the Miocene ( …

Assessment Of Energy And Hydration Demands Of Farm Workers In Mississippi, Mckenzie Lynne Hargrove

Theses and Dissertations

The agricultural community in Mississippi is critical to the state’s economy and the health of its communities. Farm workers meet these needs by performing highly demanding work with long hours of labor, limited resources, and stressful environmental conditions. The purpose of this investigation was to examine and measure indicators of physiological and behavioral adaptations to the heat such as whole body sweat rate (WBSR), sweat sodium (Na+) content, urine specific gravity (USG), and fluid intake, as well as determine total daily energy expenditure (TEE) and water influx (rH2O) via the doubly labeled water technique (DLW) in farm workers during the …

Characterizing The Microbial Structure And Function In Black Soldier Fly Digested Dairy Manure And Subsequent Larvae, Branden N. Pierce

Theses and Dissertations

This work examined the microbial structure and gene expression found in Black Soldier Fly larval guts and their frass when reared on dairy cattle manure that had been amended with either R. rhodococcus 21198, P. polymyxa 8158, or both and compare the results to larvae reared on untreated manure. First an overview of literature surrounding projected global food supplies and the possible use of insects to either increase them or mitigate the environmental effect of producing said supply. A cellulose degradation assay was performed to assist in determining which bacterial species to use as amendments. Characterization of the microbial communities …

Basics Of Forest Certification: A Primer For Forest Owners, Miah Maye Pormon, Joan U. Ureta, Marzieh Motallebi, Barry Graden

Forestry and Natural Resources

Forest certification promotes sustainable forest management, enhances market access for forest products, and improves ecosystem services. This article addresses forest owners’ hesitations in certifying their forest lands by providing information on the importance and requirements of the three biggest certification systems in the Southern U.S. This study can serve as a resource to raise awareness towards forest certification systems and encourage greater participation among forest landowners.

First Recombinant Expression, Purification, And Film Formation Of A Single Solitary Bee Silk Protein From Osmia Lignaria, Oran Wasserman, Kristin K. Durrant, Jackson J. Morley, Paula E. Oliveira, Mallory R. Wootton, Brianne E. Bell, Ethon D. Van Noy, Randolph V. Lewis, Justin A. Jones

Biology Student Research

Solitary bees account for most described bee species worldwide, with many spinning silk fibers to form protective cocoons during development; however, solitary bee silk proteins remain largely unexplored in recombinant systems and biomaterial fabrication. Here, we report the first recombinant expression and biomaterial formation from a solitary bee silk protein. Osmia lignaria silk fibroin 2 (OligF2) was expressed in Escherichia coli BL21(DE3) using an expression and purification scheme adapted from a recombinant hagfish intermediate filament (rHIF) workflow, yielding 0.34 g/L at ~70% purity. The purified OligF2 protein was cast into films at 0.75% and 1% (w/v). …