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Acute Effects Of Blood Flow Restriction During Low-Load Exercise And Recovery On Blood Flow And Performance, Shay B. Richards Jun 2025

Acute Effects Of Blood Flow Restriction During Low-Load Exercise And Recovery On Blood Flow And Performance, Shay B. Richards

Theses and Dissertations

Purpose: Use of blood flow restriction (BFR) during strength training with light loads has been used to induce similar muscular benefits as traditional strength training with heavy loads. Safe and effective recommendations for the use of BFR relies on an understanding of how different methods of BFR alter blood flow during rest and exercise. The purpose of this study was to compare blood flow during exercise and recovery between continuous BFR (CONT), intermittent BFR (INT), and no BFR (NBFR) as a control. Methods: 16 participants (8 male, 8 female) participated in this study on three different days, separated by at …


Common Aav Gene Therapy Vectors Show Nonselective Transduction Of Ex Vivo Human Brain Tissue, J P Mcginnis, Joshua Ortiz-Guzman, Maria Camila Guevara, Sai Mallannagari, Benjamin D W Belfort, Suyang Bao, Snigdha Srivastava, Maria Morkas, Emily Ji, Angela Addison, Evelyne K Tantry, Sarah Chen, Ying Wang, Zihong Chen, Kalman A Katlowitz, Jeffrey J Lange, Melissa M Blessing, Carrie A Mohila, M Cecilia Ljungberg, Guillermo Aldave, Ali Jalali, Akash Patel, Sameer A Sheth, Howard L Weiner, Shankar Gopinath, Ganesh Rao, Akdes Serin Harmanci, Daniel J Curry, Benjamin R Arenkiel Jun 2025

Common Aav Gene Therapy Vectors Show Nonselective Transduction Of Ex Vivo Human Brain Tissue, J P Mcginnis, Joshua Ortiz-Guzman, Maria Camila Guevara, Sai Mallannagari, Benjamin D W Belfort, Suyang Bao, Snigdha Srivastava, Maria Morkas, Emily Ji, Angela Addison, Evelyne K Tantry, Sarah Chen, Ying Wang, Zihong Chen, Kalman A Katlowitz, Jeffrey J Lange, Melissa M Blessing, Carrie A Mohila, M Cecilia Ljungberg, Guillermo Aldave, Ali Jalali, Akash Patel, Sameer A Sheth, Howard L Weiner, Shankar Gopinath, Ganesh Rao, Akdes Serin Harmanci, Daniel J Curry, Benjamin R Arenkiel

Children’s Nutrition Research Center Staff Publications

The ability to deliver a therapeutic sequence to a specific cell type in the human brain would make possible innumerable therapeutic options for some of our most challenging diseases; however, studies on adeno-associated virus (AAV) vector tropism have generally relied on animal models with limited translational utility. For this reason, establishing the tropism of common adeno-associated virus (AAV) vectors in living human brain tissue serves as an important baseline for further optimization, as well as a determination of human brain cell types transduced by clinically approved gene therapy vectors AAV2 and AAV9. We have adapted an


Stepping Into Motherhood: Pregnancy-Induced Changes To Foot Structure And Biomechanics, Lauren R. Williams Jun 2025

Stepping Into Motherhood: Pregnancy-Induced Changes To Foot Structure And Biomechanics, Lauren R. Williams

Theses and Dissertations

INTRODUCTION: Pregnant women have an elevated risk of developing lower limb musculoskeletal disorders, likely due to the rapid increase in body mass and joint laxity. While a few studies have evaluated changes in static foot posture due to pregnancy, it is unclear if body mass or joint laxity is the primary driver. Additionally, how pregnancy affects the mechanics of the foot during dynamic movements is still not understood. PURPOSE: The purpose of this study is to investigate the foot's response to pregnancy. First, we examined foot tissue characteristics across pregnancy, specifically plantar fascia (PF) stiffness, length, and thickness. Additionally, we …


The Effect Of Functional Training On Dynamic Knee Valgus Of Youth Soccer Players. A Pilot Study, Ole Tovar, Dóra Molnár, Badis Soussi, Ádám Uhlár, Tamás Horváth, Mira Ambrus Jun 2025

The Effect Of Functional Training On Dynamic Knee Valgus Of Youth Soccer Players. A Pilot Study, Ole Tovar, Dóra Molnár, Badis Soussi, Ádám Uhlár, Tamás Horváth, Mira Ambrus

International Journal of Exercise Science

International Journal of Exercise Science 18(7): 561-574, 2025. Knee injuries are very common in soccer players. High loads on the knee during landing or changes of direction can lead to a valgus shift of the knee, known as Dynamic Knee Valgus (DKV). Many studies have shown that a large shift in DKV is a predisposing factor for knee injuries and disease, such as anterior cruciate ligament injury (ACL), patellofemoral pain syndrome or osteoarthritis. Functional training could be a great tool to reduce DKV. Thus, in this pilot study, nine male youth soccer players (mean age: 16.4 ± 0.2 years) took …


Anthropogenic Litter In Freshwater Ecosystems: Effect On Ecosystem Processes And Spatial Distribution, Emily Johnson Jun 2025

Anthropogenic Litter In Freshwater Ecosystems: Effect On Ecosystem Processes And Spatial Distribution, Emily Johnson

Master's Theses

The rapid increase of plastic production and disposal into the environment has resulted in anthropogenic litter (i.e., trash; AL) becoming a global problem. In aquatic ecosystems, macro- and microplastics are long-lived, mobile, and interact with the physical and chemical components of aquatic ecosystems. Our objectives were to: (1) assess the impact of microplastic pollution on the ecosystem processes of ecosystem metabolism and N2 flux in an oligotrophic lake and (2) to assess the abundance and assemblage of anthropogenic litter in a large urban river. For objective (1), we added microplastics at a range of concentrations to littoral mesocosms. Ecosystem metabolism …


Arid4b: An Orchestrator From Stem Cell Fate To Carcinogenesis, Rakhee Rathnam Kalari Kandy, Madan Kumar Arumugam, Mukesh Pratap Yadav, Bibhuti Bhusan Mishra, Jyotika Sharma Jun 2025

Arid4b: An Orchestrator From Stem Cell Fate To Carcinogenesis, Rakhee Rathnam Kalari Kandy, Madan Kumar Arumugam, Mukesh Pratap Yadav, Bibhuti Bhusan Mishra, Jyotika Sharma

Faculty, Staff and Student Publications

All biological processes, from embryonic development to cancer, are tightly controlled by the interactions between genetics and epigenetics. An array of epigenetic modifications, such as DNA methylation, histone/chromatin modifications, and noncoding RNA-mediated targeting, are essential to regulate the heritable changes that occur during multiple cellular processes. A failure in proper regulation results in inappropriate gene expression that ultimately leads to pathological states. Groundbreaking advances in genomics and transcriptomics have revealed the potential involvement of epigenetics in various physiological and pathological states. The promising clinical and preclinical results shown by epigenetics drugs further underscore the central role of epigenetics in multiple …


Sickle Cell Disease Induces Chromatin Introversion And Ferroptosis In Cd8+ T Cells To Suppress Anti-Tumor Immunity, Zilong Zhao, Benxia Hu, Yalan Deng, Melinda Soeung, Jun Yao, Lanxin Bei, Yaohua Zhang, Pengju Gong, Lisa A Huang, Zhou Jiang, Jian Gao, Shuang Peng, Tina K Nguyen, Menuka Karki, Bora Lim, Cassian Yee, Jared K Burks, Qing Zhang, Li Ma, Jianjun Gao, Nizar M Tannir, Leng Han, Dihua Yu, Linghua Wang, Michael A Curran, Maria A Gubbiotti, Giannicola Genovese, Boyi Gan, Wenbo Li, Pavlos Msaouel, Liuqing Yang, Chunru Lin Jun 2025

Sickle Cell Disease Induces Chromatin Introversion And Ferroptosis In Cd8+ T Cells To Suppress Anti-Tumor Immunity, Zilong Zhao, Benxia Hu, Yalan Deng, Melinda Soeung, Jun Yao, Lanxin Bei, Yaohua Zhang, Pengju Gong, Lisa A Huang, Zhou Jiang, Jian Gao, Shuang Peng, Tina K Nguyen, Menuka Karki, Bora Lim, Cassian Yee, Jared K Burks, Qing Zhang, Li Ma, Jianjun Gao, Nizar M Tannir, Leng Han, Dihua Yu, Linghua Wang, Michael A Curran, Maria A Gubbiotti, Giannicola Genovese, Boyi Gan, Wenbo Li, Pavlos Msaouel, Liuqing Yang, Chunru Lin

Faculty, Staff and Student Publications

Understanding how genetic disorders affect CD8+ T cells in the tumor microenvironment is key to improving cancer immunotherapy. Individuals with sickle cell disease (SCD), the most prevalent inherited blood disorder, have a higher risk of developing certain cancers than the general population, but the mechanisms driving this increased risk remain unclear. Our study revealed that SCD altered CD8+ T cell 3D genome architecture, triggering ferroptosis and weakening anti-tumor immunity, thereby promoting tumor growth. Using murine and humanized SCD models, we found that disrupted chromosomal interactions in CD8+ T cells reduced the expression of anti-ferroptotic genes, including SLC7A11 and hydrogen sulfide …


Voluntary Contractions Underestimate Peak Muscle Activity In Drop Jumps, David Alan Phillips, Skylar Paletta, Michael R. Perlet Jun 2025

Voluntary Contractions Underestimate Peak Muscle Activity In Drop Jumps, David Alan Phillips, Skylar Paletta, Michael R. Perlet

Department of Kinesiology Scholarship and Creative Works

Maximal voluntary isometric contractions (MVIC) are a common method to normalize electromyographic amplitude into standardized units of %MVIC. However, in 60% of drop jump research using an MVIC in 2018–2023, supramaximal activation or activation greater than 100% MVIC occurred. Therefore, MVICs may not be representative of peak muscle activation, leading to erroneous interpretation of muscle activation. The purpose of this study is to quantify EMG normalization difference in drop jump landings. Sixteen (10 M, 6F) participants were recruited for the study. MVICs were recorded from nine lower extremity muscles and this activation compared to the maximal activation recorded from 10 …


Long-Term Nitrogen Fertilization Inhibits Carbon And Nitrogen Loss During Late-Stage Fungal Necromass Decomposition Depending On Necromass Chemistry, Lang C. Delancey, Craig R. See, Katherine Heckman, Peter G. Kennedy, Sarah E. Hobbie Jun 2025

Long-Term Nitrogen Fertilization Inhibits Carbon And Nitrogen Loss During Late-Stage Fungal Necromass Decomposition Depending On Necromass Chemistry, Lang C. Delancey, Craig R. See, Katherine Heckman, Peter G. Kennedy, Sarah E. Hobbie

Michigan Tech Publications

Fungal necromass is increasingly recognized as a key component of soil carbon (C) and nitrogen (N) cycling. However, how C and N loss from fungal necromass during decomposition is impacted by global change factors such as anthropogenic N addition and changes to soil C supply (e.g. via changing root exudation and rhizosphere priming) remains unclear and understudied relative to plant tissues. To address these gaps, we conducted a year-long decomposition experiment with four species of fungal necromass incubated across four forested sites in plots that had received inorganic N and/or labile C fertilization for two decades in Minnesota, USA. We …


Specialized Insectivores Drive Differences In Avian Community Composition Between Primary And Secondary Forest In Central Africa, Eleanor M. Barrie, Billi A. Krochuk, Crinan Jarrett, Diogo F. Ferreira, Patricia F. Rodrigues, Susana Lin Mufumu, Silvestre Esteban Malanza, Agustin Ebana Nsue Akele, Cayetano Ebana Ebana Alene, Kristin Brzeski, Jacob C. Cooper, Jared Wolfe, Luke L. Powell Jun 2025

Specialized Insectivores Drive Differences In Avian Community Composition Between Primary And Secondary Forest In Central Africa, Eleanor M. Barrie, Billi A. Krochuk, Crinan Jarrett, Diogo F. Ferreira, Patricia F. Rodrigues, Susana Lin Mufumu, Silvestre Esteban Malanza, Agustin Ebana Nsue Akele, Cayetano Ebana Ebana Alene, Kristin Brzeski, Jacob C. Cooper, Jared Wolfe, Luke L. Powell

Michigan Tech Publications

The human population of sub-Saharan Africa is projected to triple by 2100, drastically increasing anthropogenic pressure on biodiversity. When rainforest is disturbed by anthropogenic drivers, species respond heterogeneously; these patterns have rarely been quantified for Congo rainforest fauna. Our objective was to understand how community composition changed with human disturbance—with particular interest in the guilds and species that indicate primary rainforest. At a long-term bird banding site on mainland Equatorial Guinea, we captured over 3200 birds across 6 field seasons in selectively logged secondary forest and in largely undisturbed primary forest. Our multivariate ordination indicated a significant split between primary …


Influence Of Increasing Fires On Mixed Conifer Stand Dynamics In The U.S. Southwest, Simon D. Baker, Kristen M. Waring, David Auty, Nicholas Wilhelmi Jun 2025

Influence Of Increasing Fires On Mixed Conifer Stand Dynamics In The U.S. Southwest, Simon D. Baker, Kristen M. Waring, David Auty, Nicholas Wilhelmi

Aspen Bibliography

(1) Stand-replacing fires may threaten the continued stability of mixed conifer forests in the U.S. Southwest. Increasing fire frequency and severity have made post-fire forest recovery trajectories uncertain for many coniferous species, potentially leading to long-term shifts in forest structure and composition. (2) The purpose of this study was to examine post-fire stand dynamics over a 10-year period, using a network of permanent plots established prior to wildfire events across Arizona and New Mexico. We assessed changes in over story composition, regeneration, and fuel loading across different fire severities. (3) High severity fire caused near-total over story mortality, with little …


Notes On The Biology And Damage Of Two Acrobasis Species (Lepidoptera: Pyralidae) On Black Walnut In Michigan, Peter J. Martinat, William E. Wallner Jun 2025

Notes On The Biology And Damage Of Two Acrobasis Species (Lepidoptera: Pyralidae) On Black Walnut In Michigan, Peter J. Martinat, William E. Wallner

The Great Lakes Entomologist

Acrobasis juglundis (Le Baron), and A. demotella Grote cause extensive damage to black walnut foliage and branchlets, and are potential threats to commercial production of walnut trees in Michigan. Both species are univoltine in Michigan and overwinter in hibernacula. lnstars 1-111 are morphologically indistinguishable between species and have similar feeding habits. In the spring, A. demotella was active about two weeks earlier, caused more damage. and was more abundant than A. juglandis. Five parasitoids were reared from A. juglandis including Trathala plesia (Cushman), a new record. No parasitoids were reared from A. denzotella.


The Etymology Of The Beetle Name Lucanus Linnaeus (Coleoptera: Lucanidae), H. D. Cameron Jun 2025

The Etymology Of The Beetle Name Lucanus Linnaeus (Coleoptera: Lucanidae), H. D. Cameron

The Great Lakes Entomologist

[excerpt]

The handsome European stage beetle Llrcunus cervus Linnaeus is so magnificent in its outlines as to have won the attention of no less a master of drawing than Albrecht Diirer. The spectacular antler-like porrect mandibles of the male assure this creature an important place in any picture book of remarkable insects, and have awakened the linguistic imagination for metaphor. In many of the European languages it is called the flying stag or stag beetle: French cerf vulante. German Hirschkafer, Italian cervo vulante, Dutch uliegende hert, Renaissance Latin scurabelaphus (from Latin scarrcbaeus and Greek elaphus "stag").? This designation can be …


The Distribution Of The Pentatomoidea In The Northeastern Quarter Of The United States (Hemiptera), J. E. Mcpherson Jun 2025

The Distribution Of The Pentatomoidea In The Northeastern Quarter Of The United States (Hemiptera), J. E. Mcpherson

The Great Lakes Entomologist

The distributions of the pentatomoids occurring in the northeastern quarter of the United States as listed by Van Duzee in 1917 are updated.


Lighting Up The Cell: Developing A Luminescent Lanthanide Probe For Detecting Rnas, Jonathan Savell Jun 2025

Lighting Up The Cell: Developing A Luminescent Lanthanide Probe For Detecting Rnas, Jonathan Savell

Honors Projects

An anion detecting luminescent probe, Tb:DO2A-Cs124, was repurposed for use in RNA imaging. Potential RNA aptamers were identified for the molecule using Capture-SELEX and MinION sequencing. Fluorimetry data revealed these aptamers did not allow for the probe to luminesce in the presence of target RNAs due to adenosine monophosphate’s ability to quench the emission signal.


Colonization-Persistence Trade-Offs In The Human Microbiome, Liam F. Nokes Jun 2025

Colonization-Persistence Trade-Offs In The Human Microbiome, Liam F. Nokes

Environmental Studies Senior Theses

Understanding how diverse microbial taxa coexist in the human body without competitively excluding one another remains a key challenge in microbiome ecology. One proposed explanation is the colonization-persistence trade-off, where species with high colonization ability are poor persisters, and vice versa. We test for this trade-off across human associated microbial communities among many individuals and in multiple body sites using a large-scale participant-level meta-analysis of longitudinal microbiome datasets curated from the MGnify database. We applied island biogeography-based models to calculate effective colonization and persistence rates for microbial families and genera across 606 individuals over twenty-six studies. Regression of the log-transformed …


Distribution And Habitat Use Of Sounders Gray Whales From 1990 To 2023, Sara N. Mach Jun 2025

Distribution And Habitat Use Of Sounders Gray Whales From 1990 To 2023, Sara N. Mach

Honors Projects

Abstract: Every spring, a small subgroup of gray whales (Eschrichtius robustus), referred to as “Sounders,” migrate to northern Puget Sound (NPS), Washington State, to forage on small crustaceans, particularly during years of low prey availability in the Arctic, the primary foraging habitat for gray whales. In contrast to gray whales in other locations, Sounders primarily forage on ghost shrimp (Neotrypnea californiensis) while in NPS. These small crustaceans live in the sediment in the intertidal to shallow subtidal zones, so gray whales must wait for higher tides to feed. Gray whale abundance in NPS and time spent …


Patterns Of Late Holocene And Historical Extinctions On Madagascar, Laurie R. Godfrey, Zachary S. Klukkert, Brooke E. Crowley, Robin R. Dawson, Peterson Faina, Benjamin Z. Freed, Evon Hekkala, Cortni Borgerson, Harimanjaka A. M. Rasolonjatovo, Patricia C. Wright, Stephen J. Burns Jun 2025

Patterns Of Late Holocene And Historical Extinctions On Madagascar, Laurie R. Godfrey, Zachary S. Klukkert, Brooke E. Crowley, Robin R. Dawson, Peterson Faina, Benjamin Z. Freed, Evon Hekkala, Cortni Borgerson, Harimanjaka A. M. Rasolonjatovo, Patricia C. Wright, Stephen J. Burns

EKU Faculty and Staff Scholarship

Around 1000 years ago, Madagascar experienced the collapse of populations of large vertebrates that ultimately resulted in many species going extinct. The factors that led to this collapse appear to have differed regionally, but in some ways, key processes were similar across the island. This review evaluates four hypotheses that have been proposed to explain the loss of large vertebrates on Madagascar: Overkill, aridification, synergy, and subsistence shift. We explore regional differences in the paths to extinction and the significance of a prolonged extinction window across the island. The data suggest that people who arrived early and depended on hunting, …


Transition To Organic: Alfalfa Production In Utah, Earl Creech, Ryan Larsen, Marion Murray, Jennifer R. Reeve, Michele Schahczenski Jun 2025

Transition To Organic: Alfalfa Production In Utah, Earl Creech, Ryan Larsen, Marion Murray, Jennifer R. Reeve, Michele Schahczenski

All Current Publications

Transitioning to organic production may not require drastic operational changes on the part of the producer, as alfalfa production lends itself to more organic practices by default. Producers may decide to transition to certified organic production to capture organic price premiums, access niche market opportunities, reduce the use of synthetic inputs, meet growing consumer demand, enact personal values around environmental stewardship, and support soil and biodiversity conservation. Whatever a grower’s motivation, this fact sheet covers transitioning to organic production through understanding organic production systems, the National Organic Program (NOP) regulations, and crop-specific considerations.


Swi/Snf Atpase Silenced Hlf Potentiates Lung Metastasis In Solid Cancers, Jin Zhou, Austin Hepperla, Jeremy M Simon, Kangsan Kim, Qing Hu, Lei Dong, Cheng Zhang, Chengheng Liao, Alice Fang, Yayoi Adachi, Haoyong Fu, Tao Wang, Qian Liang, Fangzhou Zhao, Hongyi Liu, Masashi Takeda, Jun Fang, Hua Zhong, Peter Ly, Lu Wang, Payal Kapur, Lin Xu, Liwei Jia, Srinivas Malladi, James Brugarolas, M Celeste Simon, Bo Li, Qing Zhang Jun 2025

Swi/Snf Atpase Silenced Hlf Potentiates Lung Metastasis In Solid Cancers, Jin Zhou, Austin Hepperla, Jeremy M Simon, Kangsan Kim, Qing Hu, Lei Dong, Cheng Zhang, Chengheng Liao, Alice Fang, Yayoi Adachi, Haoyong Fu, Tao Wang, Qian Liang, Fangzhou Zhao, Hongyi Liu, Masashi Takeda, Jun Fang, Hua Zhong, Peter Ly, Lu Wang, Payal Kapur, Lin Xu, Liwei Jia, Srinivas Malladi, James Brugarolas, M Celeste Simon, Bo Li, Qing Zhang

Faculty, Staff and Student Publications

Metastasis is the main cause of cancer-related deaths, yet the underlying mechanisms remain elusive. Here, using clear cell renal cell carcinoma (ccRCC), a tumor type with frequent lung metastases, we conduct an in vivo genome-wide CRISPR-Cas9 screen and identify HLF as a potent suppressor of lung metastasis. HLF depletion enhances ccRCC cell migration and lung metastasis, whereas HLF overexpression abrogates these effects. In ccRCC patients, HLF expression is reduced at metastatic sites and associates with epigenetic silencing mediated by the SWI/SNF ATPase subunit BRG1. HLF levels negatively correlate with migration potential in collagen. Mechanistically, HLF regulates LPXN expression, modulating the …


Eph Receptors Activate Myeloid Checkpoint Receptor Lilrb5 To Support Tumor Development, Yubo He, Chengcheng Zhang, Lingxiao Tan, Mi Deng, Xiaoye Liu, Xing Yang, Qi Lou, Meng Fang, Caroline Smith, Samuel John, Wei Xiong, Xin Li, Cheryl Lewis, Jade Homsi, Ankit Gupta, Ningyan Zhang, Zhiqiang An, Cheng Cheng Zhang Jun 2025

Eph Receptors Activate Myeloid Checkpoint Receptor Lilrb5 To Support Tumor Development, Yubo He, Chengcheng Zhang, Lingxiao Tan, Mi Deng, Xiaoye Liu, Xing Yang, Qi Lou, Meng Fang, Caroline Smith, Samuel John, Wei Xiong, Xin Li, Cheryl Lewis, Jade Homsi, Ankit Gupta, Ningyan Zhang, Zhiqiang An, Cheng Cheng Zhang

Faculty, Staff and Student Publications

Immunosuppressive myeloid cells are critical obstacles to T cell-centered immune checkpoint blockade therapies, which have been successful in treating a fraction of patients with cancer. How tumor cells interact with myeloid cells to regulate immune responses and tumor development is unclear. In this study, we report that certain membrane tyrosine kinase Eph receptors, including EphA7 and EphB1, specifically bind the immune inhibitory receptors leukocyte Ig-like receptor family B 5 (LILRB5) and LILRB2. These Eph receptors induce LILRB5-mediated signaling activation, and LILRB5 also activates Eph receptor signaling. Activation of LILRB5 promoted immunosuppressive marker expression and inhibited activating marker expression on myeloid …


Mesenchymal Stem Cells And Fibroblasts Contribute To Microvascular Proliferation In Glioblastoma And Are Correlated With Immunosuppression And Poor Outcome, Candice C Poon, Shelley M Herbrich, Yulong Chen, Anwar Hossain, Gregory N Fuller, Sreyashi Basu, Marc Macaluso, Lynnette M Phillips, Joy Gumin, Zhong He, Brittany C Parker Kerrigan, Sanjay K Singh, Pratishtha Singh, Mohammed Fayyad Zaman, Derek Ng Tang, Sangeeta Goswami, Frederick F Lang, Padmanee Sharma Jun 2025

Mesenchymal Stem Cells And Fibroblasts Contribute To Microvascular Proliferation In Glioblastoma And Are Correlated With Immunosuppression And Poor Outcome, Candice C Poon, Shelley M Herbrich, Yulong Chen, Anwar Hossain, Gregory N Fuller, Sreyashi Basu, Marc Macaluso, Lynnette M Phillips, Joy Gumin, Zhong He, Brittany C Parker Kerrigan, Sanjay K Singh, Pratishtha Singh, Mohammed Fayyad Zaman, Derek Ng Tang, Sangeeta Goswami, Frederick F Lang, Padmanee Sharma

Faculty, Staff and Student Publications

Microvascular proliferation (MVP) is a disease-defining hallmark of glioblastoma and other World Health Organization grade 4 gliomas. MVP also serves as a poor prognostic marker in various solid tumors. Despite its clinical significance, the mechanisms and biological consequences of MVP are controversial and remain unclear. In this study, we performed single-cell RNA sequencing on paired CD45-CD105+ vascular/perivascular stromal cells (PVSC) and CD45+CD105± immune cells from 16 primary glioma patient samples, both with and without MVP. This analysis revealed the presence of developmentally related mesenchymal stem cells alongside cancer-associated fibroblasts, pericytes, fibromyocytes, and smooth muscle cells within the CD45-CD105+ compartment. RNA …


The Present And Future Of Precision Oncology And Tumor-Agnostic Therapeutic Approaches, Nakul M Shah, Funda Meric-Bernstam Jun 2025

The Present And Future Of Precision Oncology And Tumor-Agnostic Therapeutic Approaches, Nakul M Shah, Funda Meric-Bernstam

Faculty, Staff and Student Publications

Precision oncology has transformed the treatment landscape for patients with advanced solid tumors. Tumor-agnostic therapies, those that have been approved based on genetic mutations or biomarkers across tumor histology types, are important examples of how the implementation of precision oncology can expand therapeutic options for patients, especially those with rare cancer types and treatment-refractory disease. In this review, we first discuss how advances in next-generation sequencing and molecular profiling have enabled the identification of shared actionable alterations. Subsequently, we explore the current landscape of tumor-agnostic therapies that have received approval from the Food and Drug Administration. We discuss the strengths …


Deep Learning Based Rapid X-Ray Fluorescence Signal Extraction And Image Reconstruction For Preclinical Benchtop X-Ray Fluorescence Computed Tomography Applications, Amrit Kaphle, Sandun Jayarathna, Sang Hyun Cho Jun 2025

Deep Learning Based Rapid X-Ray Fluorescence Signal Extraction And Image Reconstruction For Preclinical Benchtop X-Ray Fluorescence Computed Tomography Applications, Amrit Kaphle, Sandun Jayarathna, Sang Hyun Cho

Faculty, Staff and Student Publications

Recent research advances have resulted in an experimental benchtop X-ray fluorescence computed tomography (XFCT) system that likely meets the imaging dose/scan time constraints for benchtop XFCT imaging of live mice injected with gold nanoparticles (GNPs). For routine in vivo benchtop XFCT imaging, however, additional challenges, most notably the need for rapid/near-real-time handling of X-ray fluorescence (XRF) signal extraction and XFCT image reconstruction, must be successfully addressed. Here we propose a novel end-to-end deep learning (DL) framework that integrates a one-dimensional convolutional neural network (1D CNN) for rapid XRF signal extraction with a U-Net model for XFCT image reconstruction. We trained …


Detecting Physical Activity Using Wearable Sensor Data, Dipok Deb Jun 2025

Detecting Physical Activity Using Wearable Sensor Data, Dipok Deb

Data Science and Data Mining

This study focuses on detecting physical activity using wearable sensor data, specifically distinguishing between walking and running. A dataset comprising accelerometer and gyroscope readings is used to train and evaluate various machine learning models, including logistic regression, random forest, k-nearest neighbors, naïve Bayes, and XGBoost. Extensive preprocessing, such as creating lag features and rolling statistics, is performed to enhance temporal data representation. The models are evaluated using metrics like accuracy, precision, recall, and F1 score. Incorporating lag and rolling features significantly improves model performance, with logistic regression achieving perfect scores across all metrics. These findings demonstrate the effectiveness of enhanced …


Interrogating The Dispersal Process And Relevant Genes In Vibrio Fischeri, Jeremy Esin Jun 2025

Interrogating The Dispersal Process And Relevant Genes In Vibrio Fischeri, Jeremy Esin

Dissertations

Biofilms are aggregates of bacteria encased in a matrix that shields bacteria from environmental hazards. These structures are formed in response to cues from the environment and drive colonization of new niches. Biofilms can be both beneficial and detrimental. Largely, research focuses on biofilms that have negative outcomes in clinical, environmental, and economical settings. However, the value of beneficial biofilms is recognized and studied. The marine bacterium Vibrio fischeri is part of a model system for studying symbiotic biofilms in a eukaryotic host. V. fischeri produces a transient biofilm at the surface of a specialized light organ and then disperses …


Restoration Of The Tumor Suppressor Function Of Y220c-Mutant P53 By Rezatapopt, A Small-Molecule Reactivator, Anna M Puzio-Kuter, Lizhong Xu, Mary Kate Mcbrayer, Romyr Dominique, Hongju H Li, Alyssa M Brown, Brandon M Russo, Chris L Mulligan, Hong Yang, Josh Battaglia, Kimberly A Robell, Dafydd H Thomas, Kuo-Sen Huang, Alexander Solovyov, Benjamin D Greenbaum, Jonathan D Oliner, Thomas W Davis, Melissa L Dumble, Melissa L Johnson, Shunbin Xiong, Peirong Yang, Guillermina Lozano, Marc M Fellous, Binh T Vu, Alison M Schram, Arnold J Levine, Masha V Poyurovsky Jun 2025

Restoration Of The Tumor Suppressor Function Of Y220c-Mutant P53 By Rezatapopt, A Small-Molecule Reactivator, Anna M Puzio-Kuter, Lizhong Xu, Mary Kate Mcbrayer, Romyr Dominique, Hongju H Li, Alyssa M Brown, Brandon M Russo, Chris L Mulligan, Hong Yang, Josh Battaglia, Kimberly A Robell, Dafydd H Thomas, Kuo-Sen Huang, Alexander Solovyov, Benjamin D Greenbaum, Jonathan D Oliner, Thomas W Davis, Melissa L Dumble, Melissa L Johnson, Shunbin Xiong, Peirong Yang, Guillermina Lozano, Marc M Fellous, Binh T Vu, Alison M Schram, Arnold J Levine, Masha V Poyurovsky

Faculty, Staff and Student Publications

Restoration of the tumor suppressor function of tumor-associated p53 mutants, including the Y220C substitution, has posed a significant challenge for therapeutic discovery. In this study, we describe rezatapopt (PC14586), part of a series of compounds designed to reactivate the p53 Y220C mutant. These compounds restore p53 tumor suppressor function by correcting its conformation and enabling it to bind DNA and activate downstream target genes, thus inducing antiproliferative changes in tumor cells. Our findings are supported by biochemical and structural analysis, in vitro and in vivo transcriptomics, and functional data, revealing the recovery of multiple aspects of the wild-type p53 program. …


Posterior Predictive Design For Phase I Clinical Trials, Chenqi Fu, Shouhao Zhou, J Jack Lee Jun 2025

Posterior Predictive Design For Phase I Clinical Trials, Chenqi Fu, Shouhao Zhou, J Jack Lee

Faculty, Staff and Student Publications

Interval-based designs represent cutting-edge adaptive methodologies for phase I clinical trials to identify the maximum tolerated dose (MTD). These designs exhibit robust performance comparable to more intricate, model-based designs, and their pretabulated decision rule enables them to be implemented as simply as the conventional algorithm-based designs. In this paper, we introduce the posterior predictive (PoP) design, a novel interval-based design that leverages advanced Bayesian predictive hypothesis testing techniques for dose escalation and de-escalation. Our work moves beyond the existing model-assisted interval-based designs by achieving global optimality in dose transition. Theoretically, the global optimality ensures that the proposed design can consistently …


Sire Evaluations: Boer, Kiko, And Spanish Kid Weaning Weights In The Southeast, Alicia D. Aldridge Jun 2025

Sire Evaluations: Boer, Kiko, And Spanish Kid Weaning Weights In The Southeast, Alicia D. Aldridge

Tennessee State University Alumni Theses and Dissertations

Sire Evaluations: Boer, Kiko, And Spanish Kid Weaning Weights in the Southeast Research was completed with weaning weight ratio (WWR) and expected progeny difference (EPD) efficiency values compared from pedigree data across six seasons (2003-2009). Four models: WWR, 90- day unadjusted, adjusted, and new adjusted WW models compared sire breed, dam breed, month of birth, dam age, sex, litter size at birth, litter size at 90 day weaning age, and birth year effects. Correction factors were re-adjusted when non-genetic effects (sex, dam age, and litter size at birth and weaning) remained significant (p<0.05) in the original 90-day adjusted WW models. The new correction factors eliminated non-genetic effects (p>0.05). Sire breed effects were not significant …


Allelopathy Of Western Red Cedar, Douglas Fir, And Western Hemlock On Seed Germination, Lainey E. Mendoza Ms. Jun 2025

Allelopathy Of Western Red Cedar, Douglas Fir, And Western Hemlock On Seed Germination, Lainey E. Mendoza Ms.

Honors Projects

Understanding the allelopathic interactions of native Pacific Northwest conifers, Douglas Fir, Western Hemlock, and Western Red Cedar is crucial to understanding the interactions of each species within their ecological niche. As of now there is little research on allelopathy of PNW conifers, specifically how different damaged states of the conifer tissue could impact the germination rates and radicle growth of a model organism. Analyzing these allelopathic interactions may be crucial to help predict forest regeneration of the PNW in the future. Presented here is the analysis of Douglas Fir, Western Hemlock, and Western Red Cedar tissue that is undamaged, damaged, …