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Articles 1621 - 1650 of 59010
Full-Text Articles in Entire DC Network
Why The Long Face? The Distribution And Niche Of The Imperiled Narrow-Faced Kangaroo Rat (Dipodomys Venustus), Garrett L. Gimbel
Why The Long Face? The Distribution And Niche Of The Imperiled Narrow-Faced Kangaroo Rat (Dipodomys Venustus), Garrett L. Gimbel
Master's Theses
Distribution models provide a practical use case of connecting ecological niche theory with species’ occurrence and assemblages. By explicitly linking Hutchinson’s “niche space” with geographic space researchers can gain valuable information on a species’ niche characteristics and interactions, thereby informing conservation and management practices. The narrow-faced kangaroo rat (Dipodomys venustus) is a highly understudied kangaroo rat species endemic to the central coast of California and is an ideal species to explore niche theory in distribution modeling. Three subspecies are currently recognized: The Santa Cruz kangaroo rat (D. v. venustus), the elephant-eared kangaroo rat (D. v. …
An Update On Novel Taxa And Revised Taxonomic Status Of Bacteria Isolated From Human Clinical Specimens Described In 2024, Arianna Carella, Karen C. Carroll, Erik Munson
An Update On Novel Taxa And Revised Taxonomic Status Of Bacteria Isolated From Human Clinical Specimens Described In 2024, Arianna Carella, Karen C. Carroll, Erik Munson
Clinical Lab Sciences Faculty Research and Publications
Knowledge of novel and revised bacterial taxonomy facilitates routine operations in the medical microbiology laboratory and communication with important stakeholders. Notable new gram-positive taxa accepted in 2024 include the clinically significant Staphylococcus brunensis sp. nov., as well as Streptococcus suis subsp. hashimotonensis subsp. nov., which can be delineated from other Streptococcus suis subsp. by possession of the Lancefield group A antigen. Four novel Enterobacterales species are discussed, plus a novel eighth family Gallaecimonadaceae. One of these, Providencia huashanensis sp. nov. is clinically significant and harbors multiple genetic determinants that encode antimicrobial resistance mechanisms. Stenotrophomonas forensis sp. nov., derived from …
Effects Of Injectable Trace Mineral Timing On Calf Performance And Health Indicators, Marie E. Goulais
Effects Of Injectable Trace Mineral Timing On Calf Performance And Health Indicators, Marie E. Goulais
Graduate Theses and Dissertations
One hundred and fourteen calves (average BW= 224kg ± 89kg) were utilized to evaluate the effects of injectable trace mineral (ITM) administration on calf performance and health when administered pre-weaning and at weaning. Calves were blocked by sex, stratified by age, and randomly assigned to one of the following treatment groups: 1) no ITM injection (CON), 2) ITM administered 28 d before weaning (PW), or 3) ITM administration at weaning (WEAN). Calves were vaccinated against respiratory pathogens on D-28 and D0. At weaning, cattle were transported to a local auction market, held overnight and returned the following morning. Body weight …
Validation Of Diagnostic Markers And Linkage Mapping In Vitis × Muscadinia Hybrids, Isabella Vaughn
Validation Of Diagnostic Markers And Linkage Mapping In Vitis × Muscadinia Hybrids, Isabella Vaughn
Graduate Theses and Dissertations
The Vitis genus comprises two subgenera, Vitis (2n = 38) and Muscadinia (2n = 40), both cultivated for wine, juice, and fresh-market fruit. Vitis vinifera cultivars produce high-quality fruit but are highly susceptible to diseases endemic to the southeastern United States, while muscadines (Muscadinia rotundifolia) provide exceptional disease resistance, heat tolerance, and environmental adaptability. Hybridizing these subgenera offers a path to combine fruit quality with disease resistance; however, wide Vitis × Muscadinia (V × M) crosses remain difficult due to differences in chromosome number, genetic divergence, and reproductive barriers that reduce fertility and recombination. Recent breeding efforts have introgressed stenospermocarpic …
Characterizing The Ethanol Sensitivity Of The Planar Cell Polarity Pathway In Endoderm Morphogenesis And Jaw Development., Raéden Omneil C. C. Rolle Gray
Characterizing The Ethanol Sensitivity Of The Planar Cell Polarity Pathway In Endoderm Morphogenesis And Jaw Development., Raéden Omneil C. C. Rolle Gray
Electronic Theses and Dissertations
Fetal Alcohol Spectrum Disorders (FASD) describes a large range of developmental defects due to prenatal alcohol exposure. It is characterized by neural and facial defects, including jaw hypoplasia. Many factors contribute to the susceptibility of ethanol-induced facial defects, including genetics, yet the genes involved, and the cell events they regulate, are poorly understood. Zebrafish have a large number of tools for genetic manipulation available, share 70% of genes with humans, are fertilized externally and are translucent during embryogenesis, making them great models to study FASD. Previous studies show that two members of the Planar Cell Polarity (PCP) pathway, zebrafish vangl2 …
Structure And Function Studies On Trna Splicing Endonuclease, Justin G. Underwood
Structure And Function Studies On Trna Splicing Endonuclease, Justin G. Underwood
Dissertations and Theses (Open Access)
A subset of eukaryotic pre-tRNAs have introns that must be spliced out to become mature molecules capable of functioning in translation. This essential processing step is performed by tRNA splicing endonuclease (TSEN), a highly conserved heterotetramer with two catalytic and two structural subunits. In addition to this conserved function, archaeal TSEN also processes pre-rRNA and yeast TSEN cleaves some mRNAs that encode mitochondrial proteins to initiate mRNA degradation. Interestingly, single amino acid mutations in TSEN cause the human Mendelian disease pontocerebellar hypoplasia (PCH) by an unknown mechanism. Recently, several groups have determined the structure of human TSEN, revealing similarities and …
The Distribution And Reproductive Success Of The Western Snowy Plover Along The Central And Southern Oregon Coast - 2025, David J. Lauten, Kathleen A. Castelein, Mary Lee, Jessica Burr, Marty R. Mccamant, Eleanor P. Gaines
The Distribution And Reproductive Success Of The Western Snowy Plover Along The Central And Southern Oregon Coast - 2025, David J. Lauten, Kathleen A. Castelein, Mary Lee, Jessica Burr, Marty R. Mccamant, Eleanor P. Gaines
Institute for Natural Resources Publications
We monitored the distribution, abundance and productivity of the federally threatened Western Snowy Plover (Anarhynchus nivosus nivosus) along the central and south coast of Oregon from 3 April – 10 September 2025. We surveyed and monitored plover activity in a project area that included, from north to south, Sutton Beach, Siltcoos River estuary, the Dunes Overlook, North Tahkenitch Creek, Tenmile Creek, Coos Bay North Spit (CBNS), Bandon Snowy Plover Management Area (SPMA), New River Habitat Restoration Area (HRA) and adjacent lands, and Floras Lake. Our objectives for the project area in 2025 were to: 1) estimate the size of the …
Farm Efficiency And Precision Agriculture Technology, Chad Fiechter, Brady Brewer, Jennifer Ifft, Michael Boehlje
Farm Efficiency And Precision Agriculture Technology, Chad Fiechter, Brady Brewer, Jennifer Ifft, Michael Boehlje
Department of Agricultural Economics Faculty Publications
Precision agriculture technology (PAT) is often viewed as a potential driver of future efficiency gains in farming. Using within-farm variation from an unbalanced panel of Kansas farms, this study examines the impact of PAT bundles on efficiency in generating gross revenue. On average, we find little evidence that these technologies improve efficiency. However, among less efficient farms, several bundles are linked to notable efficiency gains, underscoring the importance of accounting for farm heterogeneity.
An Open Letter To Leslie, Anne G. Kinne Phd
An Open Letter To Leslie, Anne G. Kinne Phd
Journal of Conscious Evolution
This article celebrates the life and work of dear friend and mentor, Allan Leslie Combs, acknowledging his continued presence in my life. Interweaving stories of our relationship with his insights on integral consciousness, Bateson’s concept of aphanipoiesis, Varela et al.’s notion of autopoiesis, and using narrative, storytelling, and autoethnographic methodologies, I tell a tale of becoming that troubles and haunts my past. The sticky tendrils of Ancestral trauma and its transhistorical epigenetic inheritance, once acutely troublesome, are shifting due in large part to the contents of this letter. Woven together, these conscious, intersecting, and interactive lenses show how Leslie has …
Comparative Analysis Of Leading Artificial Intelligence Chatbots In The Context Of Entrepreneurship, Firuz Kamalov, David Santandreu Calonge, Patrik T. Hultberg, Linda Smail, Dima Jamali
Comparative Analysis Of Leading Artificial Intelligence Chatbots In The Context Of Entrepreneurship, Firuz Kamalov, David Santandreu Calonge, Patrik T. Hultberg, Linda Smail, Dima Jamali
All Works
Artificial intelligence (AI) chatbots show remarkable abilities across applications. Despite a growing literature, their capability in the field of entrepreneurship is not fully understood. The aim of this study is to empirically evaluate and compare capabilities of five major AI chatbots—GPT-3.5, GPT-4, Gemini 1.0, Llama 2, and Claude—in the context of entrepreneurship theory, using a benchmark entrepreneurship test. In particular, the performance of the chatbots on a set of multiple-choice questions, short-answer questions, and essay questions related to entrepreneurship is assessed. The results indicate that GPT-4 delivers the strongest overall performance. Meanwhile, Llama 2 offers precise responses with a significantly …
Lstm Network-Based Scheme For Automatic Characterization Of Power Quality Disturbances, Akram Elmitwally, Mohamed Nader
Lstm Network-Based Scheme For Automatic Characterization Of Power Quality Disturbances, Akram Elmitwally, Mohamed Nader
Mansoura Engineering Journal
Recognition of power quality (PQ) troubles is a critical task in the electrical power industry. Most previous works solve the classification problem using separate feature extraction phase and classification phase. Each phase has its own techniques, and consumes a computation time. This study proposes to utilize the long short-term memory (LSTM) network as a deep learning model to classify the PQ events in one shot. The LSTM network uses its particular processing to classify a PQ event signal directly by reading its time-sequence data. Then, a dedicated post-classification algorithm (PCA) extracts start time, end time, duration, amplitude, and total harmonic …
All Rivers Reach The Sea: Can We Improve Ocean Conservation Through Improving Public Understanding Of The Connections Between Marine And Terrestrial Systems?, Lauren Hind
The Plymouth Student Scientist
With marine environments facing growing threats and their connections to terrestrial environments often disregarded, understanding public perceptions of these connections is crucial for effective ocean conservation. This study aimed to assess public understanding of the interconnectedness of marine and terrestrial environments and its influence on ocean conservation efforts. To achieve this, two questionnaires (N=560) were distributed among over 18’s (N=301) and under 18’s (N=259) evaluating their knowledge on the connections between marine and terrestrial environments, engagement with conservation and views on education and responsibility. The results indicated a limited understanding and awareness of marine-terrestrial connections, particularly among under 18’s. Current …
What Environmental Circumstances Generate Hazardous Wave Overtopping Conditions For Infrastructure Disruption: Dawlish (United Kingdom) Case Study, Zbigniew Tomasz Zielonka
What Environmental Circumstances Generate Hazardous Wave Overtopping Conditions For Infrastructure Disruption: Dawlish (United Kingdom) Case Study, Zbigniew Tomasz Zielonka
The Plymouth Student Scientist
Coastal infrastructure faces increasing threats from wave overtopping due to climate change, rising sea levels, and intensifying storms. This research investigates the environmental conditions that drive hazardous wave overtopping using Dawlish, a vulnerable section of the UK’s railway, as a case study. Understanding these conditions is essential for improving knowledge, developing effective mitigation strategies, and enhancing the resilience of shoreline defences against future challenges. Using one-year observations from WireWall instruments, offshore wave buoy, and meteorological data, the research applies a Random Forest model to assess the influence of metocean conditions on overtopping. The results indicate that wave height, tidal level, …
Assessing Overtopping Hazard To The Dawlish Railway Line: Integrating Wave Propagation Models For Coastal Metocean Condition Analysis, Matthew R. Owen
Assessing Overtopping Hazard To The Dawlish Railway Line: Integrating Wave Propagation Models For Coastal Metocean Condition Analysis, Matthew R. Owen
The Plymouth Student Scientist
With rising sea levels and increasingly frequent and intense storms due to climate change, coastal communities and structures are at an increasing risk of overtopping occurring. This study investigates the coastal metocean conditions responsible for overtopping at coastal defences, using Delft3D to model these processes with Dawlish as the case study site. Wave propagation models were employed to simulate metocean conditions from December 2021 to March 2022 to compare inshore wave conditions, alongside water level and wind parameters, with observed overtopping data from the WireWall system installed on top of the seawall, next to the railway line. The results indicate …
Seagrass Preference Among Pinfish In The Lower Laguna Madre, Nathaniel Garza
Seagrass Preference Among Pinfish In The Lower Laguna Madre, Nathaniel Garza
Theses and Dissertations
Pinfish (Lagodon rhomboides) is a common estuarine fish species found from Massachusetts to the Yucatan, Mexico. As juveniles, pinfish spend a significant portion of their life in coastal seagrasses which provide both shelter from predators and food. Along the Texas coast, there are three major species of seagrass- turtle grass (Thalassia testudinum), manatee grass (Syringodium filiforme) and shoal grass (Halodule wrightii) that differ in structural complexity. The goal of this study was to determine if pinfish associate more with one seagrass species than the others. A year-long field study was completed using twelve sample sites in the Lower Laguna Madre, …
Probing Prebiotic Rna Oligonucleotide Polymerization On A Mg-Fes Catalyst Using Microfluidics, Gabriella Garza
Probing Prebiotic Rna Oligonucleotide Polymerization On A Mg-Fes Catalyst Using Microfluidics, Gabriella Garza
Theses and Dissertations
Non-enzymatic polymerization under aqueous conditions has long been viewed as unfavorable, due to product (H2O) inhibition in abiotic settings (Damer & Deamer, 2015); despite its efficiency in biology through ATP monomer activation. Geochemically sustained pH gradients may provide free energy for condensation of organic monomers (Martin & Russell, 2003). Here, we investigated a biologically analogous non-enzymatic mechanism for RNA oligomerization, by probing the catalytic properties of a Mg-rich FeS mineral under aqueous conditions. FeS minerals promote a diverse prebiotic reaction (Wächtershäuser, 1988) and would have precipitated at Hadean submarine hydrothermal systems (Sojo et al., 2016). Natural pH and redox gradients …
Applicability Of Cementochronology To Estimate Season-Of-Death In Humans: Insights From Modern Documented Collections, Olga Ibarra-Sanchez
Applicability Of Cementochronology To Estimate Season-Of-Death In Humans: Insights From Modern Documented Collections, Olga Ibarra-Sanchez
Theses and Dissertations
Dental cementum is characterized by lifelong deposition of alternating opaque annuli in fall/winter and translucent bands in spring/summer. This study aims to test the applicability of cementochronology to estimate season-of-death, by observing the properties of the last layer, with direct implications for forensic anthropology and bioarchaeology. The sample includes canine teeth from 146 adults (84 males, 62 females), age-at-death ranging from 20-97 years (mean=61.7 years, SD=18.6 years), from two contemporary skeletal collections. Of these individuals, 78 (53.4%) died in spring/summer and 68 (46.6%) died in fall/winter. Overall accuracy was low at 64.4% (94/146). Accuracy was higher for spring/summer deaths 100.0% …
Investigating Mariculture-Associated Ecosystem Services Across The Northern Gulf Of Alaska, Josianne Haag
Investigating Mariculture-Associated Ecosystem Services Across The Northern Gulf Of Alaska, Josianne Haag
Oceanography
The rapid expansion of mariculture in Alaska can drive physical and chemical alterations to the marine environment by introducing non-native organisms or artificially increasing the biomass of a native species. Currently, the main mariculture products grown in Alaska include the Pacific oyster (Crassostrea gigas), the Pacific blue mussel (Mytilus trosuslus), and three macroalgae species: ribbon kelp (Alaria marginata), sugar kelp (Saccharina latissima), and bull kelp (Nereocystis luetkeana). This dissertation examines how these species can impact their surrounding environment to better inform management practices for this growing industry. This research characterized the resource use of C. gigas and M. trossulus seasonally …
Reliability Analysis Of In-Person And Virtual Goniometric Measurements For Select Shoulder And Forearm Motions, Autumn Whitson, Tracy Cook, Lisa Middleton, Casey E. Humphrey, Aaron D. Sciascia
Reliability Analysis Of In-Person And Virtual Goniometric Measurements For Select Shoulder And Forearm Motions, Autumn Whitson, Tracy Cook, Lisa Middleton, Casey E. Humphrey, Aaron D. Sciascia
EKU Faculty and Staff Scholarship
Background Previous research on upper extremity range of motion has compared in-person to virtual measures for sagittal plane motions (flexion/extension) showing good-excellent reliability. Since upper extremity evaluation includes motion in all planes, it is important to assess whether transverse plane motion (rotation, supination, pronation) can be reliably measured during a virtual assessment. Purpose To evaluate the reliability (test/re-test inter-rater and intra-rater) of goniometric measurements of shoulder internal rotation and forearm pronation/supination obtained in-person and virtually. Study Design Observational cohort, Reliability study Methods Subjects 18-60 years of age with no upper extremity injuries were recruited for range of motion (ROM) testing …
Functional Analysis Of Ftsa-Mediated Recruitment Of Ftsk In Escherichia Coli, Abbigale Perkins
Functional Analysis Of Ftsa-Mediated Recruitment Of Ftsk In Escherichia Coli, Abbigale Perkins
Dissertations and Theses (Open Access)
Bacterial cytokinesis requires precise coordination between cytoplasmic and periplasmic components of the divisome to ensure that membrane constriction and chromosome segregation occur in synchrony. The actin-like protein FtsA plays a central role in this process by tethering FtsZ to the membrane and recruiting downstream division proteins. Despite its essential function, the mechanisms by which FtsA coordinates divisome activation and the recruitment of late-acting proteins remain incompletely defined. To address this, the role of FtsA in activating septal synthesis and recruiting the DNA translocase FtsK was examined using mutational analysis in Escherichia coli.
The FtsAM96E R153D variant, which has …
Breeding For Disease Resistance In Melon And Watermelon, Venkata Rao Ganaparthi
Breeding For Disease Resistance In Melon And Watermelon, Venkata Rao Ganaparthi
All Dissertations
Watermelon and cantaloupe are two widely consumed cucurbits, but their acreage in the United States reduced by nearly 50% in last two decades. Fusarium wilt caused by a soil borne pathogen Fusarium oxysporum f. sp. niveum(Fon) race 2 is a major limiting factor in watermelon production as it reduces yields by 80%. Grafting watermelon plants onto the root stock of wild, disease-resistant varieties like ‘Carolina Strongback’ is the best strategy to manage Fon race 2. Though effective, this approach increases production costs.
Part of this dissertation focused on finding more sustainable, cost-effective solutions to control Fusarium wilt …
Testing Microscopic Approaches For Cementochronology In The 21st Century Identified Skeletal Collection, Portugal, Amando Luis Montalvo Jr.
Testing Microscopic Approaches For Cementochronology In The 21st Century Identified Skeletal Collection, Portugal, Amando Luis Montalvo Jr.
Theses and Dissertations
Age-at-death estimation is a key component to the creation of a biological profile in forensic anthropology. Acellular extrinsic fiber cementum annulations have been shown to correlate with chronological age. Cementochronology examines this correlation through the sum of the alternating translucent bands and opaque annuli, and the age of eruption of a tooth. While several microscopy techniques have been used, there is no consensus on the most optimal. This study will assess the reliability, precision and accuracy of three microscopy techniques: Brightfield (BF), Phase Contrast (PC) and Differential Interference Contrast (DIC). PC was the most reliable (rTEM= 10.4%), the most precise …
Taxonomy Of Endophytic Fungi Associated With Vallisneria Neotropicalis, Md. Arafat Rashid
Taxonomy Of Endophytic Fungi Associated With Vallisneria Neotropicalis, Md. Arafat Rashid
Graduate Theses and Dissertations (2019 - present)
This study presents the first comprehensive taxonomic and ecological investigation of endophytic fungi (EF) associated with the submerged aquatic macrophyte Vallisneria neotropicalis in the southern United States. Over a 12 month period, from April 2023 to March 2024, leaf samples were collected from two distinct sites in Mobile Bay, Alabama a disturbed, brackish Causeway location and a cleaner, less impacted site at Meaher State Park. Using culture dependent methods, a total of 257 fungal endophytes were isolated from 1,200 leaf segments. All isolates belonged to the phylum Ascomycota, distributed across 3 classes, 6 orders, 10 families, and 19 taxa. The …
Improved Model For Neurodegeneration In C. Elegans, Jean-Pierre Arditi
Improved Model For Neurodegeneration In C. Elegans, Jean-Pierre Arditi
Graduate Theses and Dissertations (2019 - present)
During Alzheimer's disease (AD), insoluble amyloid beta (AP) peptides accumulate to form extracellular aggregates (plaques). The direct cause of neuronal dysfunction observed in AD has been broadly investigated. The amyloid hypothesis states that AP plaques are neurotoxic, but recent studies support the AP oligomer hypothesis, which states that intracellular AP (iAP) is neurotoxic. To test this hypothesis, I used CRISPR-Cas9 gene editing to generate two transgenic Caenorhabditis elegans (C. elegans) strains. I generated a strain (RSLl 11) using the rab-3 promoter to co-express GFP in neurons, which showed no gross behavioral changes but had a 50% reduced egglaying rate. RSLl …
Twim #222: Biosensors In Bacteria, Madison Hayes, Alexis Austin, Dillon Nguyen, Angela Wilson, Rebecca Seipelt-Thiemann
Twim #222: Biosensors In Bacteria, Madison Hayes, Alexis Austin, Dillon Nguyen, Angela Wilson, Rebecca Seipelt-Thiemann
School of Medical Diagnostics & Translational Sciences Publications
Podcast annotation TWiM #222: Biosensors in Bacteria from the weekly podcast series "This Week in Microbiology" (TWiM), a podcast where experts in microbiology discuss academic papers in their field in an informal way.
Twim #286: Integrons And Invasion, Andrea Ayala-Lopez, Brittney Heist,, Thomas Salazar, Rebecca Seipelt-Thiemann, Mel Melendrez-Vallard, Angela Wilson
Twim #286: Integrons And Invasion, Andrea Ayala-Lopez, Brittney Heist,, Thomas Salazar, Rebecca Seipelt-Thiemann, Mel Melendrez-Vallard, Angela Wilson
School of Medical Diagnostics & Translational Sciences Publications
Podcast annotation TWiM #286: Integrons and Invasion from the weekly podcast series "This Week in Microbiology" (TWiM), a podcast where experts in microbiology discuss academic papers in their field in an informal way.
From Morphology To Machine Learning And Genomics: Understanding Phenotypic Variation In Wild Ducks, Sara Gonzalez
From Morphology To Machine Learning And Genomics: Understanding Phenotypic Variation In Wild Ducks, Sara Gonzalez
Open Access Theses & Dissertations
Understanding the genetic underpinning and distribution of phenotypic variation within and between divergent groups is core towards shedding light into how populations diverge and adapt, as well as how hybridization breaks or builds on these scenarios; and thus, central to evolutionary biology. In wild organisms, however, quantifying and linking phenotypic traits to underlying genetic processes, like mutation, gene expression, epigenetics and allele interactions, remains challenging. This difficulty arises from the complex interplay between morphology, environment, and gene regulation, as well as the logistical barriers of collecting and standardizing large-scale data across individuals and populations. As a result, researchers are increasingly …
Analyzing The Impact Of Diet On Dusky Gopher Frog (Lithobates Sevosus) Development In Head Start Programs, Mckalyn Nelson
Analyzing The Impact Of Diet On Dusky Gopher Frog (Lithobates Sevosus) Development In Head Start Programs, Mckalyn Nelson
Honors Theses
Species are constantly impacted by human activity, directly or indirectly. One critically endangered species under investigation is the dusky gopher frog (Lithobates sevosus), which is an endemic species of frog found in the southeastern United States. Historically found from Louisiana to Alabama, a drought in 2002 narrowed the dusky gopher frog range even further. Head start programs have been shown to be effective in increasing population numbers; however, there is not much published data on the effectiveness of captive rearing diets for these programs. The dusky gopher frog head start program at the University of Southern Mississippi’s Lake …
Advancing Trypanosoma Cruzi N-Myristoyltransferase As A Drug Target For Chagas Disease Through In Silico Discovery And Biochemical Evaluation, Diana Citlali González García
Advancing Trypanosoma Cruzi N-Myristoyltransferase As A Drug Target For Chagas Disease Through In Silico Discovery And Biochemical Evaluation, Diana Citlali González García
Open Access Theses & Dissertations
Chagas disease remains one of the most persistent and neglected parasitic infections in the Americas, advancing silently through the intracellular replication of Trypanosoma cruzi amastigotes and the invasive spread of trypomastigotes. The survival of the parasite is critically reliant on protein N-myristoylation, a lipid modification catalyzed by N-myristoyltransferase (NMT). This dissertation aims to advance beyond prior studies by systematically investigating NMT inhibition across multiple biological levels, spanning from the characterization of the purified enzyme, through cellular infection models, to the global proteomic remodeling induced within the parasite, thereby evaluating TcNMT as a potential therapeutic vulnerability in T.cruzi.Recombinant TcNMT was expressed, …
Expression And Purification Of Prohibitin Proteins And Investigation Of Their Interaction With Mycobacterium Tuberculosis’S Virulence Factors, Zeina Rhaim
Open Access Theses & Dissertations
Prohibitin (PHB) is a highly conserved mitochondrial protein involved in cell cycle regulation, apoptosis, mitochondrial integrity, macrophage activation, cytokine production and many other critical cell functions. The prohibitin complex, formed by Prohibitin-1 (PHB1) and Prohibitin-2 (PHB2), functions as a mitochondrial membrane-associated receptor. Cancer, neurodegenerative disorders, and renal conditions were found to be linked to an irregular PHB expression. Emerging evidence has also highlighted a significant role for PHB in tuberculosis (TB), particularly through its interactions with Mycobacterium tuberculosis (Mtb) virulence determinants. The molecular mechanisms by which PHB influences TB pathogenesis remain poorly understood. Our preliminary studies showed that PHB1 was …