Digital Commons Network^™

We Are Our Brains: Disembodied Brain Films In 1950s Cold War America, David P. Pierson

Faculty, Staff, and Alumni Books

Personified Body Parts in Cinema, Literature, and Visual Culture investigates the power of personifying body parts in cinema, television, visual culture, literature, erotica, folklore, and mystique.

Culturally, socially, and poetically exposing hidden aspects and subtleties of human existentialism, this book vigorously questions and problematizes numerous artistic, aesthetic, technological, naïve, and macabre manipulations of body parts for various purposes. A diverse team of authors explore how scribing human traits to limbs, eyes, brains, genitalia, hearts, and other inner organs is grotesque and aesthetic, repealing and appealing, intimidating and intimate, rude and enjoyable, material and spiritual, surprising and mundane. Personified organs are …

Multimeric Transcription Factor Bcl11a Utilizes Two Zinc-Finger Tandem Arrays To Bind Clustered Short Sequence Motifs, John R Horton, Meigen Yu, Jujun Zhou, Melody Tran, Rithvi R Anakal, Yue Lu, Robert M Blumenthal, Xiaotian Zhang, Yun Huang, Xing Zhang, Xiaodong Cheng

Faculty, Staff and Student Publications

BCL11A, a transcription factor, is vital for hematopoiesis, including B and T cell maturation and the fetal-to-adult hemoglobin switch. Mutations in BCL11A are linked to neurodevelopmental disorders. BCL11A contains two DNA-binding zinc-finger arrays, low-affinity ZF2-3 and high-affinity ZF4-6, separated by a 300-amino-acid linker. ZF2-3 and ZF4-5 share 73% identity, including five out of six DNA base-interacting residues. These arrays bind similar short sequence motifs in clusters, with the linker enabling a broader binding span. Crystallographic structures of ZF4-6, in complex with oligonucleotides from the β-globin locus region, reveal DNA sequence recognition by residues Asn756 (ZF4), Lys784 and Arg787 (ZF5). A …

Senescent Cancer-Associated Fibroblasts Mediate Immunosuppression And Promote Breast Cancer Progression, Jiayu Ye

Arts & Sciences Graduate Student Theses and Dissertations

Breast cancer is the most frequently diagnosed cancer among women worldwide, and aging is the largest risk factor for breast cancer development. The process of aging is typically accompanied by a progressive accumulation of senescent cells, which are characterized by a series of markers such as p16INKA4 (p16) positivity and a distinctive profile of secretory factors including mitogens, cytokines, and extracellular matrix (ECM) proteins that are collectively recognized as senescence-associated secretory phenotype (SASP). Numerous data so far have shown that senescent stromal cells play critical roles in modulating tumor microenvironment and progression. For example, senescent fibroblasts induced in vitro could …

The Effects Of Stress On Injury And Sickness Rates Of Middle-Distance And Distance D3 Athletes, Hailey D. Smith Ms.

Student Scholar Showcase

A runner intentionally stresses their body to experience an adaptation, however, issues occur when the body does not have time to recover. Male athletes (n = 14) on the University of Lynchburg’s distance and middle-distance team participated in this study. For 6 weeks, heart rate variability (HRV4training app), resting heart rate, perceived stress, and cortisol levels were assessed as indicators of recovery and stress. It was anticipated that indicators of physical stress such as higher resting heart rates and higher cortisol levels, would correspond with greater perceived stress and higher occurrence of injury and sickness. Cortisol (n = 2, p …

How Childhood Maltreatment Shapes Brain Regions: Considerations For Speech-Language Pathology, Alexa G. Sees

Honors Thesis

Childhood maltreatment is a pervasive issue that affects millions of children worldwide, leading to alterations in key structures such as the limbic system, basal ganglia, corpus callosum, visual cortex, and language areas. These changes result in significant cognitive, emotional, and neurological consequences, such as learning deficits, emotional regulation issues, memory problems, reduced vocabulary size, speech errors, etc. This thesis explores the neurological effects of childhood maltreatment and their implications for speech-language pathology, highlighting how maltreatment-induced brain changes affect areas within the scope of speech-language pathology, such as social communication, language development, and behavior management. These results underscore the importance of …

Zonal Hepatocellular Responses To Acute Ethanol: Impacts On Mitochondrial Function And Metabolism, Matthew T. Savoca

MUSC Theses and Dissertations

Background: Ethanol (EtOH) consumption causes hepatocellular mitochondrial depolarization (mtDepo) and mitophagy. Here, the Aims were to develop techniques to characterize sublobular hepatocellular metabolism and the distribution of mtDepo and mitophagy after acute EtOH. Methods: Wildtype and GFP-LC3 transgenic mice were treated with EtOH or vehicle and administered the covalently labeling mitochondrial membrane potential (ΔΨ) reporter, MitoTracker Red (MTR), and/or the non-covalent ΔΨ reporter, rhodamine 123 (Rh123), prior to intravital imaging, liver fixation, or hepatocyte isolation. After in vivo MTR, hepatocytes were isolated and sorted. Enrichment in E-cadherin (ECAD) and cytochrome P4502E1 in periportal (PP) and pericentral (PC) hepatocytes, respectively, validated …

Development Of A Qualitative Spot Assay For Zinc Detection In Fortified Cereal Grain Matrices, Ashley L. Lamborn

Theses and Dissertations

The present thesis is a compilation of a literature review, journal manuscript, and two standard operating procedures (SOP) on the qualitative determination of zinc in fortified cereal grain products and matrices. The literature review addresses the premise of the research as well as potential solutions for the development of a rapid qualitative spot assay for zinc determination. Quality assurance assays affirm the presence of micronutrients in food fortification initiatives, but no rapid qualitative spot methods for the assessment of zinc currently exist. Chromogenic reagents that may perform a key role in the rapid determination of zinc are identified. The journal …

Leveraging Attention Mechanism To Unlock Gene And Protein Attributes, Ala Jararweh

Computer Science ETDs

Advancing personalized medicine depends on effectively integrating and interpreting the vast, heterogeneous landscape of biological data, from genomic sequences and transcriptomics to the insights embedded in scientific literature. Current machine learning models often focus on single data modalities, limiting their capacity to capture the multifaceted nature of biological systems. We address this gap by developing three attention-based machine-learning models integrating diverse data modalities. Firstly, DeepVul is a multi-task model that leverages cancer transcriptome data to predict genes critical for cancer survival and their corresponding drugs. Subsequently, LitGene refines gene representations by integrating textual information from the scientific literature. Finally, Protein2Text …

How Bats View Their Flowers: Exploring The Use Of Visual Floral Cues By Nectarivorous Neotropical Bats, Lindsey R. Dennison

Theses

Neotropical nectar bats in cloud forest ecosystems must navigate and forage in dark, complex landscapes containing an incredible diversity of flowering plants. Despite these challenges, nectar bats are extremely successful at exploiting nectar resources from plants. Much of the sensory ecology behind these bat-plant interactions has been investigated and we know from previous studies that echolocation and olfaction are critical sensory modalities to nectar bats. Echo-acoustically conspicuous floral and vegetative structures as well as sulfur-containing volatile compounds function as signals, allowing bats to sense plants. However, not all plants exhibit conspicuous echo-acoustic and/or olfactory characteristics. Furthermore, many neotropical bat-pollinated plants …

Analysis Of Nitrogen Accumulation And Gene Expression In Hydroponically-Grown Chenopodium Genotypes, Teal Kalt

Theses and Dissertations

Nitrogen (N) is an essential macronutrient required by all plants to complete their life cycles. Inefficient N uptake contributes to over fertilization and subsequent environmental and sustainability issues resulting in the need for improved nitrogen use efficiency (NUE). Chenopodium quinoa is a resilient pseudocereal crop that is relatively adapted to N-deficient soils. Previous studies have uncovered the unique genetic structure of quinoa and its physiological responses to N availability; however, gaps remain in understanding its molecular mechanisms for NUE. This study investigates four quinoa genotypes (Real-1, 0654, KU-2, and QQ74) hydroponically grown in N-deficient conditions. RNA sequencing (RNA-seq) analysis identified …

Integrated Analysis Of Molecular And Clinical Features Associated With Overall Survival In Melanoma Patients With Brain Metastasis, Swaminathan Kumar, Meredith S Pelster, Merve Hasanov, Renato A Guerrieri, Courtney W Hudgens, Debora A Ledesma, Fuchenchu Wang, Grant M Fischer, Julie M Simon, Lauren E Haydu, Kalman V Katlowitz, Y N Vashisht Gopal, Jennifer L Mcquade, Lawrence N Kwong, Jason T Huse, Alexander J Lazar, Michael T Tetzlaff, Jeffrey E Gershenwald, Aron Y Joon, Ken Chen, Ziyi Li, Prahlad T Ram, Sherise D Ferguson, Michael A Davies

Faculty, Staff and Student Publications

Melanoma brain metastases (MBMs) are diagnosed in up to 60% of metastatic melanoma patients. Previous studies have identified clinical factors that correlate with overall survival (OS) after MBM diagnosis. However, molecular and immune features associated with OS are poorly understood. An improved understanding of the molecular and immune correlates of OS could provide insights into MBM patient outcomes and guide therapeutic development. Thus, we analyzed clinical features and outcomes of 74 melanoma patients who underwent surgical resection (via craniotomy) between 1991 and 2015 at our institution with RNA-seq data generated from their MBMs. The median post-operative OS was 8.6 months …

Determinants Of Neuronal Viability Following Spreading Depolarization, Michael C. Bennett

Biomedical Sciences ETDs

Spreading depolarizations (SDs) are massive, slowly propagating waves that cause near-complete neuroglial depolarization and are energetically demanding for the brain. Past studies have established SDs as key contributors to secondary injury in tissue that lacks the metabolic supply to meet the energetic burden of repolarization after SD. Efforts to identify the mechanisms that cause deleterious consequences following SD have been ongoing, with significant results showing that blocking excitotoxic levels of Ca²⁺ from entering neurons through NMDA receptors prevents detrimental outcomes. While these studies have advanced the field, other mechanisms may also contribute to the metabolic burden of SD. This …

Mapping Mosquito Habitats With Girl Scouts: A Citizen Science Approach Using The “Mosquito Mission: Scout & Report!” Worksheet, Claire A. Klein

Honors Program: Senior Projects (Public)

Mosquitoes, though small, impact millions around the world by being significant vectors of diseases. The search for effective mosquito control efforts is ongoing, however, there is a gap in community involvement with a focus on recognizing and reducing potential breeding areas. Public engagement is a cornerstone of effective mosquito control. This honors thesis investigates the implementation of the Mosquito Mappers program with an original Girl Scout worksheet, “Mosquito Mission: Scout & Report!” This worksheet, which utilizes NASA’s GLOBE Observer Mosquito Habitat Mapper app, enables the Girl Scouts to contribute to real-time environmental monitoring. By guiding Girl Scouts through an inquiry-based …

Biology Department Publications: 2024, Sam Lindgren

Biology Department Bibliographies

This document is a non-comprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2024. It indexes those publications available through PubMed, and serves as an archival record of the Department's robust scholarly contributions.

Heterologous Expression Of Maize-Derived Antimicrobial Peptide Zmes4 In Escherichia Coli For Potential Plant Pathogen Control, Suseno Amien, Dadi Nurdiana, Noor Istifadah, Noladhi Wicaksana, Haifanisa Salsabila

Jurnal Kultivasi

ZmES4 is a plant-derived antimicrobial peptide (AMP) from maize that shows promise as a biocontrol agent against plant pathogenic organisms. In the context of growing challenges in sustainable agriculture, AMPs like ZmES4 represent innovative alternatives to chemical pesticides. This study focuses on the structural characterization and heterologous expression of the ZmES4 peptide in Escherichia coli (E. coli). The gene encoding ZmES4 was obtained from the maize female gametophyte (NCBI Reference Sequence: NM_001112150.3) and cloned into the pET24d(+) expression vector using NcoI and XhoI restriction sites. Transformation into E. coli BL21 (DE3) cells enabled recombinant expression upon induction with isopropyl β-D-1-thiogalactopyranoside …

The Role Of Urbanization And Corticosterone In Auditory Sensory Processing In House Sparrows (Passer Domesticus), Brooke Fox, Ashley Lauraine, Hannah Peterson

24th Annual A. Paul and Carol C. Schaap Celebration of Undergraduate Research and Creative Activity (2025)

Urban habitat expansion will introduce new anthropogenic challenges for wildlife populations, including habitat fragmentation, novel predators, and pollution. These challenges may hinder animal communication and contribute to increased stress levels. This study will investigate the physiological consequences of stressors on the house sparrow (Passer domesticus). Specifically, we will examine how urbanization affects stress hormone (i.e., corticosterone, CORT) levels and its relationship to auditory processing. It is predicted that urbanization may impose novel stressors on animals, leading to differences in this hormone across urban and rural birds. We seek to understand if differences in CORT levels could impact animals' …

Local Nutrient Addition Drives Plant Diversity Losses But Not Biotic Homogenization In Global Grasslands, Qingqing Chen, Shane A. Blowes, W. Stanley Harpole, Emma Ladouceur, Elizabeth T. Borer, Andrew Macdougall, Erika Hersch-Green, Et Al.

Michigan Tech Publications

Nutrient enrichment typically causes local plant diversity declines. A common but untested expectation is that nutrient enrichment also reduces variation in nutrient conditions among localities and selects for a smaller pool of species, causing greater diversity declines at larger than local scales and thus biotic homogenization. Here we apply a framework that links changes in species richness across scales to changes in the numbers of spatially restricted and widespread species for a standardized nutrient addition experiment across 72 grasslands on six continents. Overall, we find proportionally similar species loss at local and larger scales, suggesting similar declines of spatially restricted …

Natural Killer Cells’ Functional Impairment Drives The Immune Escape Of Pre-Malignant Clones In Early-Stage Myelodysplastic Syndromes, Juan Jose Rodriguez-Sevilla, Irene Ganan-Gomez, Bijender Kumar, Natthakan Thongon, Feiyang Ma, Kelly S Chien, Yi J Kim, Hui Yang, Sanam Loghavi, Roselyn Tan, Vera Adema, Zongrui Li, Tomoyuki Tanaka, Hidetaka Uryu, Rashmi Kanagal-Shamanna, Gheath Al-Atrash, Rafael Bejar, Pinaki Prosad Banerjee, Sophia Lynn Cha, Guillermo Montalban-Bravo, Max Dougherty, Maria Claudina Fernandez Laurita, Noelle Wheeler, Baosen Jia, Eirini P Papapetrou, Franco Izzo, Daniela E Dueñas, Salome Mcallen, Yiqian Gu, Gabriele Todisco, Francesca Ficara, Matteo Giovanni Della Porta, Abhinav Jain, Koichi Takahashi, Karen Clise-Dwyer, Stephanie Halene, Maria Teresa Sabrina Bertilaccio, Guillermo Garcia-Manero, May Daher, Simona Colla

Faculty, Staff and Student Publications

Dissecting the preneoplastic disease states' biological mechanisms that precede tumorigenesis can lead to interventions that can slow down disease progression and/or mitigate disease-related comorbidities. Myelodysplastic syndromes (MDS) cannot be cured by currently available pharmacological therapies, which fail to eradicate aberrant hematopoietic stem cells (HSCs), most of which are mutated by the time of diagnosis. Here, we sought to elucidate how MDS HSCs evade immune surveillance and expand in patients with clonal cytopenias of undetermined significance (CCUS), the pre-malignant stage of MDS. We used multi-omic single-cell approaches and functional in vitro studies to show that immune escape at disease initiation is …

Large-Scale Multi-Omics Analyses In Hispanic/Latino Populations Identify Genes For Cardiometabolic Traits, Lauren E. Petty, Hung-Hsin Chen, Elizabeth G. Frankel, Wanying Zhu, Carolina G. Downie, Mariaelisa Graff, Phillip Lin, Priya Sharma, Ravi Duggirala, John Blangero

Human Genetics Publications

Here, we present a multi-omics study of type 2 diabetes and quantitative blood lipid and lipoprotein traits conducted to date in Hispanic/Latino populations (nmax = 63,184). We conduct a meta-analysis of 16 type 2 diabetes and 19 lipid trait GWAS, identifying 20 genome-wide significant loci for type 2 diabetes, including one novel locus and novel signals at two known loci, based on fine-mapping. We also identify sixty-one genome-wide significant loci across the lipid/lipoprotein traits, including nine novel loci, and novel signals at 19 known loci through fine-mapping. Next, we analyze genetically regulated expression, perform Mendelian randomization, and analyze association with …

How Can The Adoption Of Biodegradable Packaging In The Beverage Industry Specifically Contribute To Reducing Single-Use Plastic Waste, And What Regulatory Or Cost-Related Challenges Hinder Its Widespread Implementation In Developing Countries?, Rebecca Aba Gaisie

2025 Awards for Excellence in Student Research and Creative Activity - Documents

This research investigates the potential of biodegradable packaging to reduce single-use plastic waste in the beverage industry and explores the regulatory and cost-related barriers to its adoption in developing countries. The beverage sector is a major contributor to global plastic pollution, and biodegradable alternatives offer a promising path toward sustainability. Using a mixed-methods approach, this study combines qualitative interviews with industry experts and case studies of businesses implementing biodegradable solutions, alongside quantitative survey data from 100 manufacturers and 200 consumers across Africa, Asia, and Latin America. Findings reveal that while biodegradable packaging significantly reduces plastic waste and appeals to environmentally …

The Impact Of Diet-Induced Obesity On Cerebrovasculature Structure And Function, Riley M. Feagle

MUSC Theses and Dissertations

Adequate cerebral perfusion is necessary to help the brain maintain a constant and steady state of nutrient delivery. Since the brain is highly metabolic, changes in the vasculature structure can impact perfusion levels and, therefore, reduce the ability of the brain to receive the support necessary for full functionality. Vascular dysfunction is a common comorbidity among obese individuals. Given the strong association between obesity and cognitive dysfunction, understanding the correlation between reduced cerebral perfusion and altered cerebrovascular structure is crucial. The overall hypothesis of this thesis originated after ample preliminary data, and states that diet-induced obesity reduces cerebral perfusion and …

Allosteric Targeted Drug Delivery For Enhanced Blood-Brain Barrier Penetration Via Mimicking Transmembrane Domain Interactions, Kaicheng Tang, Zhongjie Tang, Miaomiao Niu, Zuyin Kuang, Weiwei Xue, Xinyu Wang, Xinlong Liu, Yang Yu, Seongdong Jeong, Yifan Ma, Annette Wu, Betty Y S Kim, Wen Jiang, Zhaogang Yang, Chong Li

Faculty, Staff and Student Publications

Current strategies for active targeting in the brain are entirely based on the effective interaction of the ligand with the orthosteric sites of specific receptors on the blood-brain barrier (BBB), which is highly susceptible to various pathophysiological factors and limits the efficacy of drug delivery. Here, we propose an allosteric targeted drug delivery strategy that targets classical BBB transmembrane receptors by designing peptide ligands that specifically bind to their transmembrane domains. This strategy prevents competitive interference from endogenous ligands and antibodies by using the insulin receptor and integrin α

Whole Genome Sequencing Analysis Of Body Mass Index Identifies Novel African Ancestry-Specific Risk Allele, Xinruo Zhang, Jennifer A. Brody, Mariaelisa Graff, Heather M. Highland, Nathalie Chami, Hanfei Xu, Zhe Wang, Kendra R. Ferrier, John Blangero, Joanne E. Curran

School of Medicine Publications

Obesity is a major public health crisis associated with high mortality rates. Previous genome-wide association studies (GWAS) investigating body mass index (BMI) have largely relied on imputed data from European individuals. This study leveraged whole-genome sequencing (WGS) data from 88,873 participants from the Trans-Omics for Precision Medicine (TOPMed) Program, of which 51% were of non-European population groups. We discovered 18 BMI-associated signals (P <  5 × 10−9), including two secondary signals. Notably, we identified and replicated a novel low-frequency single nucleotide polymorphism (SNP) in MTMR3 that was common in individuals of African descent. Using a diverse study population, we further identified two novel secondary signals in known BMI loci and pinpointed two likely causal variants in the POC5 and DMD …

Frequency Of Fruit And Vegetable Consumption Among Women Diagnosed With Gestational Diabetes, Hailey Koster

Student Research Symposium

• Consuming fruits and vegetables has been associated with a reduced risk of gestational diabetes and improved pregnancy outcomes. ¹

• However, the majority do not meet the recommendation of five servings per day or 400 g per day according to the World Health Organization.²

Reliability Of Novel Morphological Features Of The Auditory Brainstem Response, Marlana Petersen

Student Research Symposium

Pure-tone audiometry can detect hearing loss but does not determine specific site-of-lesion. It is not sensitive to many disorders, especially neural disorders that impact hearing

Sex Linkage Of Growth And Forage Attributes In Buffalograss (Buchloe Dactyloides), J A. Quinn

IGC Proceedings (1977-2023)

Buchloe dactyloides (Nutt.) Engelm. is a perennial, sod-form­ing shortgrass of central North America (Hitchcock, 1951), thriving under semiarid conditions and increasing under mod­erate to heavy grazing (Quinn, 1987). The species is largely dioecious, and sex ratios are either 1 : 1 or female-biased (Quinn and Engel, 1986). Isolated plants under controlled conditions are strictly male, strictly female, or monoecious (0 to 20 %, depending on the population). Over the last half-century, buffalograss has been widely used in central North America for reseeding depleted rangelands and for erosion control (Quinn, 1985), and recently it has received renewed attention as a turf …

Bryant University Research And Engagement Day Program

Bryant REDay (Research and Engagement Day)

No abstract provided.

Sustainable Poultry Farming: A Concept Of Iot-Based Poultry Management System For Small-Scale Farmers, Ayodeji Akinsoji Okubanjo, Ignatius Kema Okakwu, Olufemi Peter Alao, Nurudeen Samuel. Lawal, Ayoola Abiola Babalola, Abisola Olayiwola

Al-Bahir

Conventional poultry management techniques are failing to meet increased demand for poultry products as the population continues to grow. As a result, this issue has become a major concern for small-scale farmers, particularly those in low-income areas, in terms of food security. One of the main reasons for this is that the farmers rely on intensive farming methods which are inefficient for automating daily poultry operations. However, intensive farming methods pose major environmental concerns to ecosystems and poultry health. Also, the environmental conditions, welfare, and productivity of poultry operations may be harmed by the global climate crisis and poultry waste …

Economic Considerations For The Transition To Organic Farming: Insights From The 2023 Conference, Asa B. Stone, Katja Koehler-Cole, Ronald Christian Stephenson

Cornhusker Economics

Introduction

The Transition to Organic Farming Conference, led by Katja Koehler-Cole and sponsored by the Nebraska Extension and the USDA-TOPP (Transition to Organic Partnership Program), aimed to equip farmers with the essential knowledge for effective agronomic management. Focusing on overcoming challenges related to soil fertility and weed control, the conference served as a platform for both education and community building. It provided crucial insights into the economic considerations faced by farmers transitioning to organic practices, underscoring the economic complexities and benefits associated with this transition. The conference highlighted the need for comprehensive support systems to aid farmers in making informed …

Cluster Analysis To Explore Morphological Variation Of Banana (Musa Spp.): A Case Study In Jember And Lumajang, East Java, Indonesia, Halimatus Sa'diyah, Vega Kartika Sari, Riza Yuli Rusdiana, Achmad Chaqiqotul Khofi

Jurnal Kultivasi

Banana (Musa spp.) is an important horticultural plant with a high variety in Indonesia; yet, knowledge on the morphological characteristics of indigenous bananas in East Java remains inadequate. This research aims to identify the variation and classify banana cultivars in East Java based on morphological character. The exploration was conducted using the accidental sampling method. The observations include 15 qualitative and 9 quantitative characters. Descriptive statistics were used to evaluate the data, followed by cluster analysis using the average linkage method and Euclidean distance. The exploration resulted in 15 banana cultivars with varying morphology. The cluster analysis shows banana cultivars …